AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1191520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191520 MtrunA17_Chr8g0343881 95.192 312 9 3 1 306 1 312 0.0 604
Msa1191520 MtrunA17_Chr4g0070591 44.340 318 138 13 1 294 1 303 1.73e-68 217
Msa1191520 MtrunA17_Chr2g0300511 51.417 247 107 8 7 247 3 242 8.66e-65 207
Msa1191520 MtrunA17_Chr4g0021261 45.106 235 88 7 10 244 24 217 1.65e-51 172
Msa1191520 MtrunA17_Chr1g0152191 52.846 123 54 1 1 119 134 256 3.98e-41 151
Msa1191520 MtrunA17_Chr5g0409471 58.333 108 33 2 12 119 17 112 9.85e-40 141
Msa1191520 MtrunA17_Chr7g0265931 54.918 122 46 4 12 131 79 193 2.67e-36 134
Msa1191520 MtrunA17_Chr3g0127641 47.706 109 39 2 12 120 113 203 1.20e-28 115
Msa1191520 MtrunA17_Chr8g0351521 38.000 150 77 2 8 157 51 184 1.72e-21 94.0
Msa1191520 MtrunA17_Chr8g0351521 66.667 39 13 0 75 113 302 340 8.03e-13 68.6
Msa1191520 MtrunA17_Chr8g0351291 64.103 39 14 0 75 113 51 89 9.28e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1191520 AT3G13960.1 42.262 336 154 8 3 301 8 340 1.24e-69 221
Msa1191520 AT2G06200.2 57.364 129 40 3 2 117 36 162 8.25e-44 151
Msa1191520 AT2G06200.1 59.836 122 34 3 9 117 4 123 1.58e-43 149
Msa1191520 AT4G37740.1 49.342 152 55 4 12 144 165 313 1.95e-40 148
Msa1191520 AT2G22840.1 57.018 114 35 4 12 119 134 239 1.24e-35 135
Msa1191520 AT2G36400.1 51.181 127 47 4 10 127 75 195 2.70e-35 132
Msa1191520 AT3G52910.1 40.909 198 95 6 11 194 82 271 6.21e-33 125
Msa1191520 AT2G06200.3 88.235 51 4 1 69 117 1 51 1.55e-25 100
Msa1191520 AT5G53660.1 47.222 108 40 1 12 119 60 150 5.62e-25 103
Msa1191520 AT4G24150.3 41.739 115 61 2 10 119 110 223 8.23e-25 103
Msa1191520 AT4G24150.2 41.739 115 61 2 10 119 148 261 1.39e-24 103
Msa1191520 AT4G24150.1 37.143 140 57 3 10 119 148 286 1.71e-22 97.8
Msa1191520 AT2G45480.2 46.535 101 54 0 16 116 29 129 5.06e-16 77.4
Msa1191520 AT2G45480.3 37.419 155 89 1 16 162 29 183 1.15e-15 77.4
Msa1191520 AT2G45480.3 47.500 80 31 3 40 119 281 349 1.16e-14 74.3
Msa1191520 AT2G45480.1 37.419 155 89 1 16 162 27 181 1.18e-15 77.4
Msa1191520 AT2G45480.1 47.500 80 31 3 40 119 279 347 1.27e-14 74.3

Find 72 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTGAGTAAGACTTAA+AGG 0.238026 8_1:+36108005 None:intergenic
GGAGGCAAGTTCAAGGAATA+AGG 0.269692 8_1:-36108125 Msa1191520:CDS
CTTCTGCACCTCCCTGGCTC+TGG 0.278943 8_1:+36108794 None:intergenic
AAACCACTTCATCATTTCTT+TGG 0.279787 8_1:-36107861 Msa1191520:three_prime_UTR
AGCCAGGAATCTGTGTTCTT+TGG 0.341985 8_1:+36107828 None:intergenic
CTTTCACAGTACTTTGAGTC+TGG 0.352106 8_1:+36108731 None:intergenic
CTAGATGAACCTGTAGCTTC+TGG 0.361439 8_1:+36108072 None:intergenic
CCCTTTCTCTACTTCTTCCA+TGG 0.361986 8_1:-36108552 Msa1191520:CDS
GAGTAGTAGCATCTTGAGTT+TGG 0.362099 8_1:+36108429 None:intergenic
TATTTGATTAAGAGACTTGC+TGG 0.365421 8_1:-36107580 Msa1191520:three_prime_UTR
GCTGTGTTTGTGTTATTTGT+TGG 0.366262 8_1:+36108641 None:intergenic
TTCTGCACCTCCCTGGCTCT+GGG 0.368474 8_1:+36108795 None:intergenic
GAGAGTAATAGTAAGGGTTT+TGG 0.368535 8_1:+36108492 None:intergenic
GCTGCTGTTGTTGTTGTTCA+AGG 0.381104 8_1:+36108595 None:intergenic
TTAAGAGGGTTAAGAGGGGT+TGG 0.387463 8_1:+36109233 None:intergenic
GTTATGTAGGGGACCACATT+AGG 0.395401 8_1:-36107472 Msa1191520:three_prime_UTR
TGATCTTTATTGTGTTATGT+AGG 0.395473 8_1:-36107485 Msa1191520:three_prime_UTR
TATATGAGATCTGGTGGAAT+AGG 0.406485 8_1:+36109043 None:intergenic
AGAAAAGTAGACCCAGAGCC+AGG 0.407864 8_1:-36108806 Msa1191520:CDS
TCAAGTTCTTGCCATTGATT+TGG 0.410002 8_1:+36109106 None:intergenic
ATTGATTGAGAGTAATAGTA+AGG 0.410096 8_1:+36108485 None:intergenic
GAGCTTGAGTAAGACTTAAA+GGG 0.410732 8_1:+36108006 None:intergenic
GAAGAACAGATGGCAAGAAA+TGG 0.429768 8_1:-36108775 Msa1191520:CDS
ATCAAAATGTTATGTGTGGC+TGG 0.435666 8_1:-36107533 Msa1191520:three_prime_UTR
CAGAAGCTACAGGTTCATCT+AGG 0.438969 8_1:-36108071 Msa1191520:CDS
GATCTTTATTGTGTTATGTA+GGG 0.443907 8_1:-36107484 Msa1191520:three_prime_UTR
TGAGTGCAAGTTCAAGAAAT+AGG 0.444135 8_1:-36109141 Msa1191520:CDS
ACTTTCTTTCCAGAAGCTAC+AGG 0.451806 8_1:-36108081 Msa1191520:CDS
TCTGTTCTTCTGCACCTCCC+TGG 0.454328 8_1:+36108788 None:intergenic
TTTATTTAAGAGGGTTAAGA+GGG 0.462557 8_1:+36109228 None:intergenic
TTAATAGAGTATATGAGATC+TGG 0.465803 8_1:+36109034 None:intergenic
TAATCAACCTGTTGGAACTC+TGG 0.470845 8_1:+36107788 None:intergenic
CACCAAAGAACACAGATTCC+TGG 0.474579 8_1:-36107830 Msa1191520:three_prime_UTR
CTCATGATGACAAGGACTAT+AGG 0.477649 8_1:-36108376 Msa1191520:intron
CAATACTTACTTGGTGGATG+AGG 0.492982 8_1:+36108974 None:intergenic
GCCATGGAAGAAGTAGAGAA+AGG 0.495080 8_1:+36108551 None:intergenic
ACCTGTTGGAACTCTGGAGT+TGG 0.501428 8_1:+36107794 None:intergenic
TCCAACTCCAGAGTTCCAAC+AGG 0.510080 8_1:-36107795 Msa1191520:intron
GTAGAGAAAGGGAAAGAAGA+AGG 0.511856 8_1:+36108563 None:intergenic
CCATGGAAGAAGTAGAGAAA+GGG 0.516371 8_1:+36108552 None:intergenic
TGATAAAGGAGGCAAGTTCA+AGG 0.519079 8_1:-36108132 Msa1191520:intron
GAGGCAAGTTCAAGGAATAA+GGG 0.523074 8_1:-36108124 Msa1191520:CDS
GTTCCACAGGCTTTCTTGAA+CGG 0.529120 8_1:+36108687 None:intergenic
ATCTTTATTGTGTTATGTAG+GGG 0.533482 8_1:-36107483 Msa1191520:three_prime_UTR
TGTGAATGGTGCAGTTGGGT+GGG 0.535441 8_1:-36108852 Msa1191520:intron
TTCATCATTTCTTTGGTGAG+TGG 0.536164 8_1:-36107854 Msa1191520:three_prime_UTR
GTTGGAAGCAAGATCTAGCC+AGG 0.536185 8_1:+36107812 None:intergenic
GTGAATGGTGCAGTTGGGTG+GGG 0.550789 8_1:-36108851 Msa1191520:intron
GAAAAGTAGACCCAGAGCCA+GGG 0.561252 8_1:-36108805 Msa1191520:CDS
ATTATTGTACTGTGCATGGA+TGG 0.567364 8_1:+36109258 None:intergenic
TCACCAAAGAAATGATGAAG+TGG 0.571081 8_1:+36107858 None:intergenic
TCAAAATGTTATGTGTGGCT+GGG 0.576383 8_1:-36107532 Msa1191520:three_prime_UTR
CTGTGAATGGTGCAGTTGGG+TGG 0.577092 8_1:-36108853 Msa1191520:intron
GGAGATTATTGTACTGTGCA+TGG 0.577269 8_1:+36109254 None:intergenic
ATAGAGTATATGAGATCTGG+TGG 0.579210 8_1:+36109037 None:intergenic
GAAGAAACTACTAGTTCCAC+AGG 0.590035 8_1:+36108674 None:intergenic
CAAGAAATGGAGATGCTCAA+AGG 0.592887 8_1:-36108762 Msa1191520:CDS
TTGATTGAGAGTAATAGTAA+GGG 0.594306 8_1:+36108486 None:intergenic
GGGAGGTGCAGAAGAACAGA+TGG 0.600412 8_1:-36108785 Msa1191520:CDS
ATCTTATTCTCATGATGACA+AGG 0.605341 8_1:-36108384 Msa1191520:CDS
TGACTGATCATGACAAGCCA+TGG 0.611257 8_1:+36108535 None:intergenic
CAAAATGTTATGTGTGGCTG+GGG 0.614659 8_1:-36107531 Msa1191520:three_prime_UTR
AGGAATAAGGGATGGTACTG+TGG 0.625865 8_1:-36108112 Msa1191520:CDS
TTGATTAAGAGACTTGCTGG+TGG 0.637573 8_1:-36107577 Msa1191520:three_prime_UTR
CAAGTTCAAGGAATAAGGGA+TGG 0.642231 8_1:-36108120 Msa1191520:CDS
GCTGTTGTTGTTGTTCAAGG+AGG 0.650068 8_1:+36108598 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.653157 8_1:-36108690 Msa1191520:CDS
AAGTAGACCCAGAGCCAGGG+AGG 0.659120 8_1:-36108802 Msa1191520:CDS
GTCAACAAGAACAACAAACA+AGG 0.662924 8_1:-36107905 Msa1191520:CDS
TTATTTAAGAGGGTTAAGAG+GGG 0.668991 8_1:+36109229 None:intergenic
AAAGATCAAAATGTTATGTG+TGG 0.684063 8_1:-36107537 Msa1191520:three_prime_UTR
TGTGAAAGACACATGCACAG+AGG 0.772839 8_1:-36108716 Msa1191520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATATTTATATCAAATG+CGG - chr8_1:36108471-36108490 Msa1191520:CDS 10.0%
!!! TTTTATATTTATATCAAATG+CGG - chr8_1:36108471-36108490 Msa1191520:CDS 10.0%
!!! TGATTTTAAATTGTATTTAG+AGG + chr8_1:36108529-36108548 None:intergenic 15.0%
!! TTTATATACTTTATTATCCA+TGG - chr8_1:36109139-36109158 Msa1191520:CDS 15.0%
!!! TGATTTTAAATTGTATTTAG+AGG + chr8_1:36108529-36108548 None:intergenic 15.0%
!! TTTATATACTTTATTATCCA+TGG - chr8_1:36109139-36109158 Msa1191520:CDS 15.0%
!!! AAAAATAAGGATTTTCTTGA+TGG + chr8_1:36107582-36107601 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG + chr8_1:36107595-36107614 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG + chr8_1:36107687-36107706 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG + chr8_1:36107688-36107707 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG - chr8_1:36108416-36108435 Msa1191520:CDS 20.0%
!! ACCATGGTATATATATTTAT+TGG + chr8_1:36108420-36108439 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG + chr8_1:36109009-36109028 None:intergenic 20.0%
!!! AAAAATAAGGATTTTCTTGA+TGG + chr8_1:36107582-36107601 None:intergenic 20.0%
!!! TTTTTTGAGACACAAAAATA+AGG + chr8_1:36107595-36107614 None:intergenic 20.0%
!! AACCATGTATTTAAAAACTA+GGG + chr8_1:36107687-36107706 None:intergenic 20.0%
!! TAACCATGTATTTAAAAACT+AGG + chr8_1:36107688-36107707 None:intergenic 20.0%
!! ACCAATAAATATATATACCA+TGG - chr8_1:36108416-36108435 Msa1191520:CDS 20.0%
!! ACCATGGTATATATATTTAT+TGG + chr8_1:36108420-36108439 None:intergenic 20.0%
!! GAACAAAAAAACAAAAACAT+AGG + chr8_1:36109009-36109028 None:intergenic 20.0%
! TTTATTTAAGAGGGTTAAGA+GGG + chr8_1:36107534-36107553 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG + chr8_1:36107535-36107554 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG + chr8_1:36107543-36107562 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG + chr8_1:36107544-36107563 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG - chr8_1:36107689-36107708 Msa1191520:intron 25.0%
! TTAATAGAGTATATGAGATC+TGG + chr8_1:36107728-36107747 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG + chr8_1:36107794-36107813 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG + chr8_1:36107797-36107816 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG + chr8_1:36108276-36108295 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG + chr8_1:36108277-36108296 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG - chr8_1:36108613-36108632 Msa1191520:CDS 25.0%
! ATAAATGATAATCAACCTGT+TGG + chr8_1:36108982-36109001 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG + chr8_1:36109117-36109136 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG + chr8_1:36109214-36109233 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG - chr8_1:36109222-36109241 Msa1191520:five_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG - chr8_1:36109274-36109293 Msa1191520:five_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG - chr8_1:36109275-36109294 Msa1191520:five_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG - chr8_1:36109276-36109295 Msa1191520:five_prime_UTR 25.0%
! TTTATTTAAGAGGGTTAAGA+GGG + chr8_1:36107534-36107553 None:intergenic 25.0%
!!! TTTTATTTAAGAGGGTTAAG+AGG + chr8_1:36107535-36107554 None:intergenic 25.0%
!!! GAAGATGCTTTTATTTAAGA+GGG + chr8_1:36107543-36107562 None:intergenic 25.0%
!!! TGAAGATGCTTTTATTTAAG+AGG + chr8_1:36107544-36107563 None:intergenic 25.0%
!! GTTTTTAAATACATGGTTAC+TGG - chr8_1:36107689-36107708 Msa1191520:intron 25.0%
! TTAATAGAGTATATGAGATC+TGG + chr8_1:36107728-36107747 None:intergenic 25.0%
! TTATAACAATACTTACTTGG+TGG + chr8_1:36107794-36107813 None:intergenic 25.0%
! GAGTTATAACAATACTTACT+TGG + chr8_1:36107797-36107816 None:intergenic 25.0%
! TTGATTGAGAGTAATAGTAA+GGG + chr8_1:36108276-36108295 None:intergenic 25.0%
! ATTGATTGAGAGTAATAGTA+AGG + chr8_1:36108277-36108296 None:intergenic 25.0%
!! TTGTACTTGATTGTTGATAA+AGG - chr8_1:36108613-36108632 Msa1191520:CDS 25.0%
! ATAAATGATAATCAACCTGT+TGG + chr8_1:36108982-36109001 None:intergenic 25.0%
! CATTAACGCAATAGAAAAAA+CGG + chr8_1:36109117-36109136 None:intergenic 25.0%
!!! ATTTTGATCTTTAACTCTCT+TGG + chr8_1:36109214-36109233 None:intergenic 25.0%
! AAAGATCAAAATGTTATGTG+TGG - chr8_1:36109222-36109241 Msa1191520:five_prime_UTR 25.0%
! TGATCTTTATTGTGTTATGT+AGG - chr8_1:36109274-36109293 Msa1191520:five_prime_UTR 25.0%
! GATCTTTATTGTGTTATGTA+GGG - chr8_1:36109275-36109294 Msa1191520:five_prime_UTR 25.0%
! ATCTTTATTGTGTTATGTAG+GGG - chr8_1:36109276-36109295 Msa1191520:five_prime_UTR 25.0%
TTATTTAAGAGGGTTAAGAG+GGG + chr8_1:36107533-36107552 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG - chr8_1:36107682-36107701 Msa1191520:intron 30.0%
! TTTGTTGTCATAACTGTGAA+TGG - chr8_1:36107893-36107912 Msa1191520:exon 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG + chr8_1:36108302-36108321 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG - chr8_1:36108375-36108394 Msa1191520:intron 30.0%
GTATTTAGAGGTCTCTATAA+CGG + chr8_1:36108517-36108536 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG - chr8_1:36108616-36108635 Msa1191520:CDS 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG - chr8_1:36108819-36108838 Msa1191520:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG - chr8_1:36108898-36108917 Msa1191520:intron 30.0%
TATTTGATTAAGAGACTTGC+TGG - chr8_1:36109179-36109198 Msa1191520:five_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG - chr8_1:36109249-36109268 Msa1191520:five_prime_UTR 30.0%
TTATTTAAGAGGGTTAAGAG+GGG + chr8_1:36107533-36107552 None:intergenic 30.0%
!! AGCCCTAGTTTTTAAATACA+TGG - chr8_1:36107682-36107701 Msa1191520:intron 30.0%
! TTTGTTGTCATAACTGTGAA+TGG - chr8_1:36107893-36107912 Msa1191520:exon 30.0%
!!! ATGATCAAGTGTTTTAGAAG+AGG + chr8_1:36108302-36108321 None:intergenic 30.0%
ATCTTATTCTCATGATGACA+AGG - chr8_1:36108375-36108394 Msa1191520:intron 30.0%
GTATTTAGAGGTCTCTATAA+CGG + chr8_1:36108517-36108536 None:intergenic 30.0%
! TACTTGATTGTTGATAAAGG+AGG - chr8_1:36108616-36108635 Msa1191520:CDS 30.0%
!!! GAACAACACTGTTTTGTTTT+AGG - chr8_1:36108819-36108838 Msa1191520:CDS 30.0%
AAACCACTTCATCATTTCTT+TGG - chr8_1:36108898-36108917 Msa1191520:intron 30.0%
TATTTGATTAAGAGACTTGC+TGG - chr8_1:36109179-36109198 Msa1191520:five_prime_UTR 30.0%
! GGTGAATTTTCTTTAGCAAA+AGG - chr8_1:36109249-36109268 Msa1191520:five_prime_UTR 30.0%
ATTATTGTACTGTGCATGGA+TGG + chr8_1:36107504-36107523 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG - chr8_1:36107618-36107637 Msa1191520:intron 35.0%
! CTCTTTTCACACCAAATCAA+TGG - chr8_1:36107642-36107661 Msa1191520:intron 35.0%
! TCAAGTTCTTGCCATTGATT+TGG + chr8_1:36107656-36107675 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG + chr8_1:36107719-36107738 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG + chr8_1:36107725-36107744 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG - chr8_1:36107929-36107948 Msa1191520:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG + chr8_1:36108121-36108140 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG + chr8_1:36108266-36108285 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG + chr8_1:36108270-36108289 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG - chr8_1:36108345-36108364 Msa1191520:intron 35.0%
AAGATGTTGAGTCCAAAAAG+AGG + chr8_1:36108360-36108379 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG + chr8_1:36108436-36108455 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG + chr8_1:36108756-36108775 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG + chr8_1:36108757-36108776 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG - chr8_1:36108854-36108873 Msa1191520:intron 35.0%
TCACCAAAGAAATGATGAAG+TGG + chr8_1:36108904-36108923 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG - chr8_1:36108905-36108924 Msa1191520:intron 35.0%
CAATAGAAAAAACGGACAGA+CGG + chr8_1:36109109-36109128 None:intergenic 35.0%
ATCAAAATGTTATGTGTGGC+TGG - chr8_1:36109226-36109245 Msa1191520:five_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG - chr8_1:36109227-36109246 Msa1191520:five_prime_UTR 35.0%
!!! GGACCACATTAGGATTTTTT+TGG - chr8_1:36109297-36109316 Msa1191520:five_prime_UTR 35.0%
ATTATTGTACTGTGCATGGA+TGG + chr8_1:36107504-36107523 None:intergenic 35.0%
TGAGTGCAAGTTCAAGAAAT+AGG - chr8_1:36107618-36107637 Msa1191520:intron 35.0%
! CTCTTTTCACACCAAATCAA+TGG - chr8_1:36107642-36107661 Msa1191520:intron 35.0%
! TCAAGTTCTTGCCATTGATT+TGG + chr8_1:36107656-36107675 None:intergenic 35.0%
TATATGAGATCTGGTGGAAT+AGG + chr8_1:36107719-36107738 None:intergenic 35.0%
ATAGAGTATATGAGATCTGG+TGG + chr8_1:36107725-36107744 None:intergenic 35.0%
!!! GGATGTTTTGAAATGGGATT+TGG - chr8_1:36107929-36107948 Msa1191520:CDS 35.0%
!! GCTGTGTTTGTGTTATTTGT+TGG + chr8_1:36108121-36108140 None:intergenic 35.0%
!! GTAATAGTAAGGGTTTTGGT+AGG + chr8_1:36108266-36108285 None:intergenic 35.0%
! GAGAGTAATAGTAAGGGTTT+TGG + chr8_1:36108270-36108289 None:intergenic 35.0%
! TACTACTCATCACCTCTTTT+TGG - chr8_1:36108345-36108364 Msa1191520:intron 35.0%
AAGATGTTGAGTCCAAAAAG+AGG + chr8_1:36108360-36108379 None:intergenic 35.0%
! GAGACAACAATTTAGAACCA+TGG + chr8_1:36108436-36108455 None:intergenic 35.0%
GAGCTTGAGTAAGACTTAAA+GGG + chr8_1:36108756-36108775 None:intergenic 35.0%
TGAGCTTGAGTAAGACTTAA+AGG + chr8_1:36108757-36108776 None:intergenic 35.0%
GTCAACAAGAACAACAAACA+AGG - chr8_1:36108854-36108873 Msa1191520:intron 35.0%
TCACCAAAGAAATGATGAAG+TGG + chr8_1:36108904-36108923 None:intergenic 35.0%
TTCATCATTTCTTTGGTGAG+TGG - chr8_1:36108905-36108924 Msa1191520:intron 35.0%
CAATAGAAAAAACGGACAGA+CGG + chr8_1:36109109-36109128 None:intergenic 35.0%
ATCAAAATGTTATGTGTGGC+TGG - chr8_1:36109226-36109245 Msa1191520:five_prime_UTR 35.0%
TCAAAATGTTATGTGTGGCT+GGG - chr8_1:36109227-36109246 Msa1191520:five_prime_UTR 35.0%
GGAGATTATTGTACTGTGCA+TGG + chr8_1:36107508-36107527 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG + chr8_1:36107788-36107807 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG - chr8_1:36107902-36107921 Msa1191520:CDS 40.0%
TAACTGTGAATGGTGCAGTT+GGG - chr8_1:36107903-36107922 Msa1191520:CDS 40.0%
GAAGAACAGATGGCAAGAAA+TGG - chr8_1:36107984-36108003 Msa1191520:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG - chr8_1:36107997-36108016 Msa1191520:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG + chr8_1:36108031-36108050 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG + chr8_1:36108088-36108107 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG + chr8_1:36108199-36108218 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG + chr8_1:36108210-36108229 None:intergenic 40.0%
GAGTAGTAGCATCTTGAGTT+TGG + chr8_1:36108333-36108352 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG - chr8_1:36108383-36108402 Msa1191520:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG - chr8_1:36108627-36108646 Msa1191520:CDS 40.0%
GAGGCAAGTTCAAGGAATAA+GGG - chr8_1:36108635-36108654 Msa1191520:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG - chr8_1:36108639-36108658 Msa1191520:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG - chr8_1:36108678-36108697 Msa1191520:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG + chr8_1:36108974-36108993 None:intergenic 40.0%
ATACTTTACGAGCATCCAGA+CGG - chr8_1:36109071-36109090 Msa1191520:CDS 40.0%
ATAACTTCACTCATCTGCCA+TGG + chr8_1:36109159-36109178 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG - chr8_1:36109182-36109201 Msa1191520:five_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG - chr8_1:36109228-36109247 Msa1191520:five_prime_UTR 40.0%
GGAGATTATTGTACTGTGCA+TGG + chr8_1:36107508-36107527 None:intergenic 40.0%
! CAATACTTACTTGGTGGATG+AGG + chr8_1:36107788-36107807 None:intergenic 40.0%
ATAACTGTGAATGGTGCAGT+TGG - chr8_1:36107902-36107921 Msa1191520:CDS 40.0%
TAACTGTGAATGGTGCAGTT+GGG - chr8_1:36107903-36107922 Msa1191520:CDS 40.0%
GAAGAACAGATGGCAAGAAA+TGG - chr8_1:36107984-36108003 Msa1191520:CDS 40.0%
CAAGAAATGGAGATGCTCAA+AGG - chr8_1:36107997-36108016 Msa1191520:CDS 40.0%
CTTTCACAGTACTTTGAGTC+TGG + chr8_1:36108031-36108050 None:intergenic 40.0%
GAAGAAACTACTAGTTCCAC+AGG + chr8_1:36108088-36108107 None:intergenic 40.0%
GTAGAGAAAGGGAAAGAAGA+AGG + chr8_1:36108199-36108218 None:intergenic 40.0%
CCATGGAAGAAGTAGAGAAA+GGG + chr8_1:36108210-36108229 None:intergenic 40.0%
GAGTAGTAGCATCTTGAGTT+TGG + chr8_1:36108333-36108352 None:intergenic 40.0%
CTCATGATGACAAGGACTAT+AGG - chr8_1:36108383-36108402 Msa1191520:CDS 40.0%
TGATAAAGGAGGCAAGTTCA+AGG - chr8_1:36108627-36108646 Msa1191520:CDS 40.0%
GAGGCAAGTTCAAGGAATAA+GGG - chr8_1:36108635-36108654 Msa1191520:CDS 40.0%
CAAGTTCAAGGAATAAGGGA+TGG - chr8_1:36108639-36108658 Msa1191520:CDS 40.0%
ACTTTCTTTCCAGAAGCTAC+AGG - chr8_1:36108678-36108697 Msa1191520:CDS 40.0%
TAATCAACCTGTTGGAACTC+TGG + chr8_1:36108974-36108993 None:intergenic 40.0%
ATACTTTACGAGCATCCAGA+CGG - chr8_1:36109071-36109090 Msa1191520:CDS 40.0%
ATAACTTCACTCATCTGCCA+TGG + chr8_1:36109159-36109178 None:intergenic 40.0%
!! TTGATTAAGAGACTTGCTGG+TGG - chr8_1:36109182-36109201 Msa1191520:five_prime_UTR 40.0%
CAAAATGTTATGTGTGGCTG+GGG - chr8_1:36109228-36109247 Msa1191520:five_prime_UTR 40.0%
TTAAGAGGGTTAAGAGGGGT+TGG + chr8_1:36107529-36107548 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG - chr8_1:36107922-36107941 Msa1191520:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG - chr8_1:36107923-36107942 Msa1191520:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG - chr8_1:36108043-36108062 Msa1191520:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr8_1:36108069-36108088 Msa1191520:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG + chr8_1:36108075-36108094 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG + chr8_1:36108164-36108183 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG + chr8_1:36108167-36108186 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG - chr8_1:36108207-36108226 Msa1191520:intron 45.0%
GCCATGGAAGAAGTAGAGAA+AGG + chr8_1:36108211-36108230 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG + chr8_1:36108227-36108246 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG - chr8_1:36108634-36108653 Msa1191520:CDS 45.0%
AGGAATAAGGGATGGTACTG+TGG - chr8_1:36108647-36108666 Msa1191520:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG + chr8_1:36108690-36108709 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG - chr8_1:36108688-36108707 Msa1191520:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG - chr8_1:36108929-36108948 Msa1191520:intron 45.0%
AGCCAGGAATCTGTGTTCTT+TGG + chr8_1:36108934-36108953 None:intergenic 45.0%
TACTTTACGAGCATCCAGAC+GGG - chr8_1:36109072-36109091 Msa1191520:CDS 45.0%
GTTATGTAGGGGACCACATT+AGG - chr8_1:36109287-36109306 Msa1191520:five_prime_UTR 45.0%
TTAAGAGGGTTAAGAGGGGT+TGG + chr8_1:36107529-36107548 None:intergenic 45.0%
!!! TGGGTGGGGATGTTTTGAAA+TGG - chr8_1:36107922-36107941 Msa1191520:CDS 45.0%
!!! GGGTGGGGATGTTTTGAAAT+GGG - chr8_1:36107923-36107942 Msa1191520:CDS 45.0%
TGTGAAAGACACATGCACAG+AGG - chr8_1:36108043-36108062 Msa1191520:CDS 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr8_1:36108069-36108088 Msa1191520:CDS 45.0%
GTTCCACAGGCTTTCTTGAA+CGG + chr8_1:36108075-36108094 None:intergenic 45.0%
! GCTGTTGTTGTTGTTCAAGG+AGG + chr8_1:36108164-36108183 None:intergenic 45.0%
! GCTGCTGTTGTTGTTGTTCA+AGG + chr8_1:36108167-36108186 None:intergenic 45.0%
CCCTTTCTCTACTTCTTCCA+TGG - chr8_1:36108207-36108226 Msa1191520:intron 45.0%
GCCATGGAAGAAGTAGAGAA+AGG + chr8_1:36108211-36108230 None:intergenic 45.0%
TGACTGATCATGACAAGCCA+TGG + chr8_1:36108227-36108246 None:intergenic 45.0%
GGAGGCAAGTTCAAGGAATA+AGG - chr8_1:36108634-36108653 Msa1191520:CDS 45.0%
AGGAATAAGGGATGGTACTG+TGG - chr8_1:36108647-36108666 Msa1191520:CDS 45.0%
CTAGATGAACCTGTAGCTTC+TGG + chr8_1:36108690-36108709 None:intergenic 45.0%
CAGAAGCTACAGGTTCATCT+AGG - chr8_1:36108688-36108707 Msa1191520:CDS 45.0%
CACCAAAGAACACAGATTCC+TGG - chr8_1:36108929-36108948 Msa1191520:intron 45.0%
AGCCAGGAATCTGTGTTCTT+TGG + chr8_1:36108934-36108953 None:intergenic 45.0%
TACTTTACGAGCATCCAGAC+GGG - chr8_1:36109072-36109091 Msa1191520:CDS 45.0%
GTTATGTAGGGGACCACATT+AGG - chr8_1:36109287-36109306 Msa1191520:five_prime_UTR 45.0%
!!! TTTTTAATATTTTATGAAAA+AGG - chr8_1:36107817-36107836 Msa1191520:three_prime_UTR 5.0%
!! AATAATATTAAATAACTTTA+TGG + chr8_1:36109051-36109070 None:intergenic 5.0%
!!! TTTTTAATATTTTATGAAAA+AGG - chr8_1:36107817-36107836 Msa1191520:three_prime_UTR 5.0%
!! AATAATATTAAATAACTTTA+TGG + chr8_1:36109051-36109070 None:intergenic 5.0%
TGTGAATGGTGCAGTTGGGT+GGG - chr8_1:36107907-36107926 Msa1191520:CDS 50.0%
AGAAAAGTAGACCCAGAGCC+AGG - chr8_1:36107953-36107972 Msa1191520:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG - chr8_1:36107954-36107973 Msa1191520:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG + chr8_1:36108950-36108969 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG - chr8_1:36108964-36108983 Msa1191520:intron 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG + chr8_1:36108968-36108987 None:intergenic 50.0%
TGTGAATGGTGCAGTTGGGT+GGG - chr8_1:36107907-36107926 Msa1191520:CDS 50.0%
AGAAAAGTAGACCCAGAGCC+AGG - chr8_1:36107953-36107972 Msa1191520:CDS 50.0%
GAAAAGTAGACCCAGAGCCA+GGG - chr8_1:36107954-36107973 Msa1191520:CDS 50.0%
GTTGGAAGCAAGATCTAGCC+AGG + chr8_1:36108950-36108969 None:intergenic 50.0%
TCCAACTCCAGAGTTCCAAC+AGG - chr8_1:36108964-36108983 Msa1191520:intron 50.0%
!! ACCTGTTGGAACTCTGGAGT+TGG + chr8_1:36108968-36108987 None:intergenic 50.0%
CTGTGAATGGTGCAGTTGGG+TGG - chr8_1:36107906-36107925 Msa1191520:CDS 55.0%
GTGAATGGTGCAGTTGGGTG+GGG - chr8_1:36107908-36107927 Msa1191520:CDS 55.0%
TCTGTTCTTCTGCACCTCCC+TGG + chr8_1:36107974-36107993 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG - chr8_1:36107974-36107993 Msa1191520:CDS 55.0%
CTGTGAATGGTGCAGTTGGG+TGG - chr8_1:36107906-36107925 Msa1191520:CDS 55.0%
GTGAATGGTGCAGTTGGGTG+GGG - chr8_1:36107908-36107927 Msa1191520:CDS 55.0%
TCTGTTCTTCTGCACCTCCC+TGG + chr8_1:36107974-36107993 None:intergenic 55.0%
! GGGAGGTGCAGAAGAACAGA+TGG - chr8_1:36107974-36107993 Msa1191520:CDS 55.0%
AAGTAGACCCAGAGCCAGGG+AGG - chr8_1:36107957-36107976 Msa1191520:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG + chr8_1:36107967-36107986 None:intergenic 60.0%
!! CGGACACAGAAGTACCCGTC+TGG + chr8_1:36109089-36109108 None:intergenic 60.0%
AAGTAGACCCAGAGCCAGGG+AGG - chr8_1:36107957-36107976 Msa1191520:CDS 60.0%
! TTCTGCACCTCCCTGGCTCT+GGG + chr8_1:36107967-36107986 None:intergenic 60.0%
!! CGGACACAGAAGTACCCGTC+TGG + chr8_1:36109089-36109108 None:intergenic 60.0%
CTTCTGCACCTCCCTGGCTC+TGG + chr8_1:36107968-36107987 None:intergenic 65.0%
CTTCTGCACCTCCCTGGCTC+TGG + chr8_1:36107968-36107987 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_1 gene 36107465 36109316 36107465 ID=Msa1191520;Name=Msa1191520
chr8_1 mRNA 36107465 36109316 36107465 ID=Msa1191520-mRNA-1;Parent=Msa1191520;Name=Msa1191520-mRNA-1;_AED=0.04;_eAED=0.04;_QI=149|1|0.75|1|0.66|0.5|4|250|306
chr8_1 exon 36107465 36107616 36107465 ID=Msa1191520-mRNA-1:exon:11938;Parent=Msa1191520-mRNA-1
chr8_1 exon 36107796 36108146 36107796 ID=Msa1191520-mRNA-1:exon:11937;Parent=Msa1191520-mRNA-1
chr8_1 exon 36108377 36108860 36108377 ID=Msa1191520-mRNA-1:exon:11936;Parent=Msa1191520-mRNA-1
chr8_1 exon 36108984 36109316 36108984 ID=Msa1191520-mRNA-1:exon:11935;Parent=Msa1191520-mRNA-1
chr8_1 five_prime_UTR 36109168 36109316 36109168 ID=Msa1191520-mRNA-1:five_prime_utr;Parent=Msa1191520-mRNA-1
chr8_1 CDS 36108984 36109167 36108984 ID=Msa1191520-mRNA-1:cds;Parent=Msa1191520-mRNA-1
chr8_1 CDS 36108377 36108860 36108377 ID=Msa1191520-mRNA-1:cds;Parent=Msa1191520-mRNA-1
chr8_1 CDS 36107894 36108146 36107894 ID=Msa1191520-mRNA-1:cds;Parent=Msa1191520-mRNA-1
chr8_1 three_prime_UTR 36107796 36107893 36107796 ID=Msa1191520-mRNA-1:three_prime_utr;Parent=Msa1191520-mRNA-1
chr8_1 three_prime_UTR 36107465 36107616 36107465 ID=Msa1191520-mRNA-1:three_prime_utr;Parent=Msa1191520-mRNA-1
Gene Sequence

>Msa1191520

ATGATGAGTGCAAGTTCAAGAAATAGGTCTCTTTTCACACCAAATCAATGGCAAGAACTTGAACAACAAGCCCTAGTTTTTAAATACATGGTTACTGGAACACCTATTCCACCAGATCTCATATACTCTATTAAGAGAAGTTTAGACACTTCAATATCTTCAAGAATCTTTCCTCATCCACCAATTGGGTGGGGATGTTTTGAAATGGGATTTGGCAGAAAAGTAGACCCAGAGCCAGGGAGGTGCAGAAGAACAGATGGCAAGAAATGGAGATGCTCAAAGGAAGCATATCCAGACTCAAAGTACTGTGAAAGACACATGCACAGAGGTAGAAACCGTTCAAGAAAGCCTGTGGAACTAGTAGTTTCTTCTTCAACAACACCAACAAATAACACAAACACAGCATCTTCTTACAACAGAAACACCTCCTTGAACAACAACAACAGCAGCATAAACTCACCTTCTTCTTTCCCTTTCTCTACTTCTTCCATGGCTTGTCATGATCAGTCACAATCTTTTTCACAATCCTACCAAAACCCTTACTATTACTCTCAATCAATTACCTCTTCTAAAACACTTGATCATTCTGATTTCCAAACTCAAGATGCTACTACTCATCACCTCTTTTTGGACTCAACATCTTATTCTCATGATGACAAGGACTATAGGAGGCAAGTTCAAGGAATAAGGGATGGTACTGTGGATGAGAGAACTTTCTTTCCAGAAGCTACAGGTTCATCTAGGAGCTGCTATCATGATTCATATCAACAACAACTATCAATGAATCCCTTTAAGTCTTACTCAAGCTCACAGTTTCAAAATATCAATGATGATAATTCAAGACAACAACAAGAACAACACTGTTTTGTTTTAGGCACTGACATCAAGTCAACAAGAACAACAAACAAGGACAGAAAGTGA
Protein sequence

>Msa1191520

MMSASSRNRSLFTPNQWQELEQQALVFKYMVTGTPIPPDLIYSIKRSLDTSISSRIFPHPPIGWGCFEMGFGRKVDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGRNRSRKPVELVVSSSTTPTNNTNTASSYNRNTSLNNNNSSINSPSSFPFSTSSMACHDQSQSFSQSYQNPYYYSQSITSSKTLDHSDFQTQDATTHHLFLDSTSYSHDDKDYRRQVQGIRDGTVDERTFFPEATGSSRSCYHDSYQQQLSMNPFKSYSSSQFQNINDDNSRQQQEQHCFVLGTDIKSTRTTNKDRK*