AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0483750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0483750 MtrunA17_Chr5g0405791 100.000 197 0 0 1 197 1 197 2.40e-147 407
Msa0483750 MtrunA17_Chr8g0371501 97.462 197 5 0 1 197 1 197 1.10e-143 397
Msa0483750 MtrunA17_Chr4g0037021 89.340 197 21 0 1 197 1 197 1.93e-133 372
Msa0483750 MtrunA17_Chr3g0117931 77.835 194 42 1 2 194 4 197 1.41e-116 330
Msa0483750 MtrunA17_Chr2g0325101 84.270 178 28 0 1 178 1 178 8.01e-116 327
Msa0483750 MtrunA17_Chr6g0486041 74.874 199 47 1 1 196 1 199 1.40e-111 317
Msa0483750 MtrunA17_Chr4g0046211 88.112 143 17 0 1 143 1 143 5.57e-94 270
Msa0483750 MtrunA17_Chr7g0265001 30.851 188 116 5 8 192 18 194 1.09e-25 98.6
Msa0483750 MtrunA17_Chr1g0182321 31.383 188 116 6 6 191 15 191 7.56e-25 96.7
Msa0483750 MtrunA17_Chr1g0182301 31.383 188 116 6 6 191 15 191 1.31e-24 95.9
Msa0483750 MtrunA17_Chr1g0212521 32.386 176 106 6 6 179 15 179 1.60e-24 95.9
Msa0483750 MtrunA17_Chr4g0030911 32.386 176 106 6 6 179 15 179 2.78e-24 95.1
Msa0483750 MtrunA17_Chr7g0263071 30.851 188 117 6 6 191 15 191 3.86e-24 94.7
Msa0483750 MtrunA17_Chr8g0384311 30.726 179 109 5 6 181 13 179 2.64e-23 92.4
Msa0483750 MtrunA17_Chr3g0130271 29.630 189 119 5 7 192 13 190 3.95e-23 92.0
Msa0483750 MtrunA17_Chr1g0199331 31.461 178 105 7 7 180 32 196 8.76e-23 90.9
Msa0483750 MtrunA17_Chr1g0180291 30.899 178 108 5 7 181 15 180 1.44e-22 90.5
Msa0483750 MtrunA17_Chr8g0349711 30.814 172 105 5 1 170 1 160 2.85e-22 89.7
Msa0483750 MtrunA17_Chr8g0335471 33.516 182 102 8 6 180 8 177 4.21e-22 89.0
Msa0483750 MtrunA17_Chr1g0159481 30.726 179 109 5 6 181 17 183 4.99e-22 89.4
Msa0483750 MtrunA17_Chr7g0275991 33.939 165 95 5 8 170 22 174 5.37e-22 89.4
Msa0483750 MtrunA17_Chr4g0055651 29.947 187 112 6 6 185 8 182 6.03e-22 88.6
Msa0483750 MtrunA17_Chr8g0390561 31.111 180 109 6 8 184 11 178 1.42e-21 87.4
Msa0483750 MtrunA17_Chr7g0250531 31.016 187 110 7 6 185 8 182 1.53e-21 87.4
Msa0483750 MtrunA17_Chr4g0055081 32.727 165 97 5 8 170 11 163 2.05e-21 87.4
Msa0483750 MtrunA17_Chr2g0276931 30.857 175 104 6 1 170 1 163 2.79e-21 87.0
Msa0483750 MtrunA17_Chr3g0109721 32.961 179 105 5 8 183 12 178 8.98e-21 85.5
Msa0483750 MtrunA17_Chr2g0319951 29.282 181 114 5 6 184 13 181 1.01e-20 85.9
Msa0483750 MtrunA17_Chr5g0400131 32.727 165 97 5 8 170 11 163 1.97e-20 84.7
Msa0483750 MtrunA17_Chr2g0327911 29.891 184 114 6 6 185 15 187 2.55e-20 84.7
Msa0483750 MtrunA17_Chr8g0346421 38.655 119 70 3 8 124 28 145 2.68e-20 85.1
Msa0483750 MtrunA17_Chr6g0458081 27.624 181 117 5 1 179 1 169 2.72e-20 84.3
Msa0483750 MtrunA17_Chr2g0290891 27.907 172 110 5 1 170 1 160 3.85e-20 84.0
Msa0483750 MtrunA17_Chr8g0349681 30.233 172 106 5 1 170 8 167 8.83e-20 86.3
Msa0483750 MtrunA17_Chr8g0349681 29.651 172 107 5 1 170 379 538 5.21e-19 84.3
Msa0483750 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 170 329 1.22e-18 83.2
Msa0483750 MtrunA17_Chr5g0433561 31.515 165 99 4 8 170 12 164 2.27e-19 81.6
Msa0483750 MtrunA17_Chr4g0040781 35.606 132 82 3 6 135 13 143 3.82e-19 81.6
Msa0483750 MtrunA17_Chr1g0201121 30.729 192 116 7 1 184 1 183 4.18e-19 81.3
Msa0483750 MtrunA17_Chr3g0123971 30.909 165 100 4 8 170 11 163 4.46e-19 81.3
Msa0483750 MtrunA17_Chr4g0046261 29.834 181 113 5 6 184 13 181 4.61e-19 81.3
Msa0483750 MtrunA17_Chr2g0276941 30.994 171 104 5 6 174 15 173 4.87e-19 81.3
Msa0483750 MtrunA17_Chr3g0112681 29.834 181 113 5 6 184 12 180 5.46e-19 81.3
Msa0483750 MtrunA17_Chr2g0315551 35.833 120 74 3 6 123 13 131 5.74e-19 81.3
Msa0483750 MtrunA17_Chr1g0174281 32.530 166 98 5 7 170 16 169 6.21e-19 81.3
Msa0483750 MtrunA17_Chr8g0382221 31.515 165 99 5 8 170 11 163 6.56e-19 80.9
Msa0483750 MtrunA17_Chr2g0283131 35.833 120 74 3 6 123 13 131 1.05e-18 80.5
Msa0483750 MtrunA17_Chr1g0197331 28.804 184 116 5 7 186 16 188 1.31e-18 80.1
Msa0483750 MtrunA17_Chr7g0234201 28.947 190 121 5 6 193 13 190 1.69e-18 79.7
Msa0483750 MtrunA17_Chr7g0231131 31.928 166 99 6 7 170 16 169 2.65e-18 79.3
Msa0483750 MtrunA17_Chr8g0381841 31.515 165 99 5 8 170 19 171 6.75e-18 78.6
Msa0483750 MtrunA17_Chr8g0392931 35.294 119 74 3 6 122 14 131 1.19e-17 77.8
Msa0483750 MtrunA17_Chr2g0331691 29.032 186 117 6 6 185 10 186 1.38e-17 77.4
Msa0483750 MtrunA17_Chr7g0214241 28.947 190 121 5 6 193 15 192 1.53e-17 77.4
Msa0483750 MtrunA17_Chr4g0034871 27.957 186 119 5 6 185 8 184 2.64e-17 76.6
Msa0483750 MtrunA17_Chr3g0123821 31.325 166 98 6 8 170 18 170 3.04e-17 76.6
Msa0483750 MtrunA17_Chr6g0466301 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0483750 MtrunA17_Chr6g0465491 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0483750 MtrunA17_Chr4g0032201 29.341 167 104 5 6 170 12 166 5.92e-17 75.9
Msa0483750 MtrunA17_Chr1g0189361 30.114 176 105 6 6 177 14 175 6.15e-17 75.9
Msa0483750 MtrunA17_Chr8g0351861 31.138 167 101 6 6 170 16 170 6.27e-17 75.9
Msa0483750 MtrunA17_Chr2g0325091 36.667 120 73 3 6 123 11 129 1.74e-16 74.3
Msa0483750 MtrunA17_Chr4g0004661 28.497 193 121 7 1 185 57 240 2.52e-16 74.7
Msa0483750 MtrunA17_Chr8g0383261 28.235 170 109 3 6 170 12 173 2.58e-16 73.9
Msa0483750 MtrunA17_Chr6g0482721 33.065 124 80 3 6 127 12 134 9.61e-16 72.4
Msa0483750 MtrunA17_Chr7g0259771 28.877 187 99 4 7 180 9 174 4.67e-15 72.8
Msa0483750 MtrunA17_Chr3g0138121 28.485 165 103 4 7 170 14 164 6.94e-14 67.4
Msa0483750 MtrunA17_Chr3g0138101 28.485 165 103 4 7 170 14 164 7.16e-14 67.4
Msa0483750 MtrunA17_Chr3g0138111 28.485 165 103 4 7 170 14 164 8.00e-14 67.4
Msa0483750 MtrunA17_Chr1g0185591 27.604 192 95 6 7 180 13 178 8.38e-14 69.3
Msa0483750 MtrunA17_Chr1g0191811 28.571 175 115 4 7 180 17 182 8.40e-13 66.2
Msa0483750 MtrunA17_Chr4g0046221 70.270 37 11 0 142 178 10 46 2.23e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0483750 AT2G17800.2 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0483750 AT2G17800.1 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0483750 AT3G51300.1 95.431 197 9 0 1 197 1 197 1.40e-127 357
Msa0483750 AT4G35950.1 95.939 197 8 0 1 197 1 197 1.42e-127 357
Msa0483750 AT4G35020.1 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0483750 AT4G35020.2 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0483750 AT4G35020.3 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0483750 AT1G75840.1 91.573 178 15 0 1 178 1 178 5.36e-122 343
Msa0483750 AT1G20090.1 90.341 176 17 0 3 178 2 177 3.09e-119 336
Msa0483750 AT5G45970.1 86.034 179 25 0 1 179 1 179 1.71e-117 332
Msa0483750 AT4G28950.1 75.500 200 43 2 1 196 1 198 2.63e-111 317
Msa0483750 AT5G62880.1 80.447 179 35 0 2 180 4 182 8.69e-111 315
Msa0483750 AT3G48040.1 80.447 179 35 0 2 180 4 182 8.96e-110 313
Msa0483750 AT2G44690.1 75.000 192 48 0 6 197 18 209 2.40e-106 304
Msa0483750 AT5G62880.2 79.070 172 32 1 2 173 4 171 5.27e-103 295
Msa0483750 AT3G48040.2 80.368 163 32 0 2 164 4 166 4.32e-100 287
Msa0483750 AT3G51290.2 95.122 164 8 0 34 197 635 798 1.77e-96 297
Msa0483750 AT4G35020.4 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0483750 AT4G35020.5 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0483750 AT3G46060.2 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0483750 AT3G46060.3 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0483750 AT3G46060.1 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0483750 AT3G54840.2 33.333 168 97 6 7 171 34 189 8.90e-24 93.6
Msa0483750 AT3G54840.1 32.143 168 99 5 7 171 34 189 9.53e-24 93.6
Msa0483750 AT3G54840.3 31.928 166 98 5 9 171 2 155 3.62e-23 91.3
Msa0483750 AT1G02130.1 32.544 169 100 6 6 172 8 164 8.47e-23 91.3
Msa0483750 AT3G53610.2 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0483750 AT3G53610.3 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0483750 AT3G53610.1 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0483750 AT5G45130.1 33.333 165 96 4 8 170 12 164 1.10e-22 90.9
Msa0483750 AT5G59840.1 31.737 167 101 5 6 170 15 170 1.43e-22 90.9
Msa0483750 AT4G19640.1 33.333 165 96 4 8 170 12 164 2.49e-22 90.1
Msa0483750 AT1G43890.3 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0483750 AT1G43890.1 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0483750 AT1G43890.2 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0483750 AT1G16920.1 32.418 182 109 6 6 185 13 182 2.99e-22 90.1
Msa0483750 AT5G45130.2 33.129 163 95 4 10 170 22 172 5.58e-22 89.4
Msa0483750 AT1G73640.1 32.984 191 110 6 6 190 13 191 1.97e-21 88.6
Msa0483750 AT2G44610.1 33.333 165 96 5 8 170 11 163 2.27e-21 87.8
Msa0483750 AT5G03530.2 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0483750 AT5G03530.1 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0483750 AT4G18430.1 32.597 181 108 5 6 184 13 181 4.12e-21 87.0
Msa0483750 AT4G35860.1 27.624 181 117 5 1 179 1 169 1.11e-20 85.9
Msa0483750 AT4G17530.1 31.016 187 110 7 6 185 8 182 1.79e-20 85.1
Msa0483750 AT4G39890.1 34.731 167 92 6 8 170 11 164 1.82e-20 85.5
Msa0483750 AT5G47200.1 31.319 182 106 7 6 180 8 177 3.03e-20 84.7
Msa0483750 AT3G11730.1 29.940 167 103 5 6 170 8 162 3.37e-20 84.3
Msa0483750 AT2G22290.1 33.333 165 96 5 8 170 11 163 3.58e-20 84.3
Msa0483750 AT1G06400.1 32.335 167 99 5 6 170 13 167 6.72e-20 84.0
Msa0483750 AT4G19640.2 36.885 122 74 3 8 127 12 132 8.89e-20 82.0
Msa0483750 AT4G18800.1 36.667 120 73 3 6 123 13 131 9.53e-20 83.6
Msa0483750 AT1G09630.1 31.492 181 110 5 6 184 12 180 9.58e-20 83.6
Msa0483750 AT3G15060.1 31.285 179 109 5 8 184 15 181 1.65e-19 82.8
Msa0483750 AT4G17170.1 27.326 172 111 5 1 170 1 160 2.17e-19 82.4
Msa0483750 AT4G19640.3 34.074 135 86 3 8 140 12 145 2.68e-19 82.0
Msa0483750 AT2G31680.1 29.670 182 114 4 6 185 12 181 2.75e-19 82.4
Msa0483750 AT3G09900.1 32.934 167 99 6 6 170 15 170 3.29e-19 82.0
Msa0483750 AT3G07410.1 30.526 190 118 5 6 193 12 189 3.29e-19 82.0
Msa0483750 AT4G17160.1 26.744 172 112 5 1 170 1 160 3.69e-19 81.6
Msa0483750 AT1G18200.1 30.387 181 112 6 6 184 13 181 5.73e-19 81.6
Msa0483750 AT5G60860.1 30.387 181 112 5 6 184 13 181 5.85e-19 81.6
Msa0483750 AT2G43130.1 29.121 182 115 5 6 185 12 181 5.89e-19 81.3
Msa0483750 AT3G12160.1 32.530 166 98 5 7 170 16 169 5.91e-19 81.6
Msa0483750 AT5G45750.1 35.833 120 74 3 6 123 13 131 6.31e-19 81.3
Msa0483750 AT3G18820.1 29.570 186 116 6 6 185 8 184 7.28e-19 80.9
Msa0483750 AT5G03520.1 33.533 167 98 6 6 170 15 170 8.63e-19 80.9
Msa0483750 AT1G01200.1 34.132 167 95 6 7 170 29 183 1.66e-18 80.9
Msa0483750 AT1G28550.1 37.500 120 72 3 6 123 13 131 1.71e-18 80.1
Msa0483750 AT5G10260.1 32.121 165 98 5 8 170 11 163 2.11e-18 79.7
Msa0483750 AT1G05810.2 30.994 171 104 5 6 174 58 216 2.97e-18 80.5
Msa0483750 AT1G05810.1 29.670 182 114 5 6 185 12 181 3.26e-18 79.7
Msa0483750 AT2G33870.1 37.815 119 71 3 6 122 13 130 5.00e-18 79.0
Msa0483750 AT5G64990.1 33.333 165 96 5 8 170 9 161 7.57e-18 78.2
Msa0483750 AT5G47520.1 28.652 178 113 5 2 177 10 175 1.85e-17 77.4
Msa0483750 AT1G22740.1 29.167 192 119 6 1 184 1 183 2.47e-17 77.0
Msa0483750 AT1G52280.1 30.726 179 107 7 1 171 1 170 5.25e-17 76.3
Msa0483750 AT2G21880.1 27.174 184 121 4 6 184 9 184 9.54e-17 75.5
Msa0483750 AT4G39990.1 32.787 122 79 3 7 126 18 138 1.10e-16 75.5
Msa0483750 AT1G07410.1 34.454 119 75 3 6 122 12 129 1.27e-16 75.1
Msa0483750 AT5G59150.1 29.240 171 107 6 6 174 12 170 1.57e-16 75.1
Msa0483750 AT3G16100.1 29.947 187 114 7 1 179 1 178 1.87e-16 74.7
Msa0483750 AT5G47960.1 33.051 118 76 3 7 122 16 132 2.16e-16 74.7
Msa0483750 AT5G65270.1 28.729 181 113 6 7 184 18 185 2.31e-16 74.7
Msa0483750 AT4G09720.1 30.409 171 104 6 6 170 8 169 5.43e-16 73.2
Msa0483750 AT3G09910.3 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0483750 AT3G09910.1 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0483750 AT3G09910.2 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0483750 AT5G27540.1 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0483750 AT5G27540.2 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0483750 AT3G46830.1 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0483750 AT3G46830.2 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0483750 AT1G49300.2 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0483750 AT1G49300.1 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0483750 AT5G64990.2 31.977 172 96 6 8 170 9 168 3.92e-15 71.2
Msa0483750 AT5G55080.1 30.769 117 79 2 7 122 14 129 6.81e-15 70.9
Msa0483750 AT3G09910.5 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0483750 AT3G09910.4 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0483750 AT4G09720.4 28.409 176 106 5 6 170 8 174 1.45e-14 69.7
Msa0483750 AT3G63150.1 30.286 175 112 5 7 180 15 180 2.34e-14 71.2
Msa0483750 AT5G20020.1 28.485 165 103 4 7 170 14 164 8.48e-14 67.8
Msa0483750 AT5G20010.1 28.485 165 103 4 7 170 14 164 9.29e-14 67.8
Msa0483750 AT5G55190.1 31.092 119 80 2 7 124 14 131 9.68e-14 67.4
Msa0483750 AT4G09720.3 27.473 182 106 5 6 170 8 180 1.17e-13 67.4
Msa0483750 AT5G03520.2 30.137 146 90 5 47 191 47 181 1.91e-12 63.9
Msa0483750 AT4G35860.2 25.373 134 87 4 47 179 2 123 1.00e-11 61.2

Find 81 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAGATCTGAGGCTATTT+TGG 0.133154 3_4:-501891 Msa0483750:five_prime_UTR
CTTTGTTCCAACCAGAATTA+TGG 0.197915 3_4:+500428 None:intergenic
AGAAAGAGAAAGAACCTATT+TGG 0.261379 3_4:+502175 None:intergenic
AGTGCGAATGTGGTTGTTAA+TGG 0.319066 3_4:-500882 Msa0483750:CDS
AATCACAACTTCGTTCAAAA+AGG 0.320947 3_4:-502295 Msa0483750:five_prime_UTR
TGAAATCAATAATAATAATA+AGG 0.321196 3_4:-502342 None:intergenic
TAATGGAAGTATTGTGAATC+TGG 0.328007 3_4:-500865 Msa0483750:CDS
TCTTCTGCTTTGGTGGTTGA+AGG 0.339738 3_4:+499513 None:intergenic
AAACGACATTTCTTGTCATT+TGG 0.355939 3_4:-499173 None:intergenic
TTTGTTCCAACCAGAATTAT+GGG 0.374664 3_4:+500429 None:intergenic
TGGTTGAAGGACAACTCTTA+TGG 0.386728 3_4:+499526 None:intergenic
TTATGAAAATGTCTCCAAAA+AGG 0.394383 3_4:-500647 Msa0483750:intron
AAAGAAGGGGAGGGGAATAC+AGG 0.394999 3_4:+499412 None:intergenic
CACACTCTCAGCTTCCAAAT+AGG 0.400756 3_4:-502189 Msa0483750:five_prime_UTR
TTCTTTGTTATTGAAGAAGT+TGG 0.406930 3_4:-499381 Msa0483750:three_prime_UTR
TTGTATCGAGATAGACCTTC+GGG 0.409474 3_4:-499873 Msa0483750:intron
TTAAGCCACCTTATGCTTCA+AGG 0.411941 3_4:+499316 None:intergenic
ATCACCTGAGCTGTGGTAAT+GGG 0.421707 3_4:+499812 None:intergenic
GAAGAATAAGAAGAAAGTAC+TGG 0.425254 3_4:-501934 Msa0483750:five_prime_UTR
AACCGTGGGGAAAGTATTGC+TGG 0.429322 3_4:+501772 None:intergenic
CAAAGAAAATGATGAAAAGA+AGG 0.438811 3_4:+499397 None:intergenic
TAAGAAGAAAGTACTGGAGG+AGG 0.446434 3_4:-501928 Msa0483750:five_prime_UTR
AAAGAAGAATAAAGCACAAA+AGG 0.449553 3_4:-499489 Msa0483750:CDS
AATCACCTGAGCTGTGGTAA+TGG 0.454383 3_4:+499811 None:intergenic
TAATTGCCTTCTTCTCTCAC+AGG 0.456105 3_4:-502144 Msa0483750:five_prime_UTR
ACCTGGTGTCCCCATAATTC+TGG 0.467734 3_4:-500439 Msa0483750:CDS
CTAGATCTCCTTGAAGCATA+AGG 0.474002 3_4:-499324 Msa0483750:three_prime_UTR
AAGAGGATATACGAAATAGT+TGG 0.475651 3_4:-502254 Msa0483750:five_prime_UTR
AAAGAAAATGATGAAAAGAA+GGG 0.477205 3_4:+499398 None:intergenic
GGTGCATAATGCTTCAACTC+TGG 0.481713 3_4:+500459 None:intergenic
GGAGGAGGGGAACTGTCCTC+AGG 0.484285 3_4:-501913 Msa0483750:five_prime_UTR
AAGAAGAAAGTACTGGAGGA+GGG 0.485036 3_4:-501927 Msa0483750:five_prime_UTR
AGTGAATTACAATATTGAAC+AGG 0.490190 3_4:+499466 None:intergenic
CCTAACCCTGTGAGAGAAGA+AGG 0.494457 3_4:+502138 None:intergenic
ATTCTGGTTGGAACAAAGCT+TGG 0.495346 3_4:-500423 Msa0483750:intron
TTTGTATCGAGATAGACCTT+CGG 0.498207 3_4:-499874 Msa0483750:intron
GTGGTAATGGGAACGGCACC+AGG 0.503169 3_4:+499824 None:intergenic
TATTGTGAATCTGGGTTTGT+GGG 0.507703 3_4:-500856 Msa0483750:CDS
AATGGAAGTATTGTGAATCT+GGG 0.518446 3_4:-500864 Msa0483750:CDS
AATGATGAAAAGAAGGGGAG+GGG 0.524862 3_4:+499404 None:intergenic
CTGGGTTTGTGGGATACTGC+TGG 0.538632 3_4:-500846 Msa0483750:intron
GTATTGTGAATCTGGGTTTG+TGG 0.538694 3_4:-500857 Msa0483750:CDS
CGTTCCCATTACCACAGCTC+AGG 0.544810 3_4:-499816 Msa0483750:intron
TGAATGCAGTTCAAAATCAC+AGG 0.553565 3_4:-499665 Msa0483750:CDS
AAATGTGTTACTGTTGGTGA+TGG 0.556572 3_4:-501830 Msa0483750:CDS
TTTATTAAATGTGTTACTGT+TGG 0.563795 3_4:-501836 Msa0483750:CDS
CACCAGCAATACTTTCCCCA+CGG 0.567542 3_4:-501774 Msa0483750:intron
GAACTGCATTCAATGTAAGC+TGG 0.572826 3_4:+499676 None:intergenic
AAATGATGAAAAGAAGGGGA+GGG 0.575158 3_4:+499403 None:intergenic
CAGTTCTGCATAGACCATCC+TGG 0.577967 3_4:-499842 Msa0483750:CDS
AAGAAAATGATGAAAAGAAG+GGG 0.582810 3_4:+499399 None:intergenic
GTTCAATATTGTAATTCACT+AGG 0.584735 3_4:-499463 Msa0483750:exon
GAATAAGAAGAAAGTACTGG+AGG 0.584899 3_4:-501931 Msa0483750:five_prime_UTR
AATTGCCTTCTTCTCTCACA+GGG 0.587915 3_4:-502143 Msa0483750:intron
AAAATGATGAAAAGAAGGGG+AGG 0.591003 3_4:+499402 None:intergenic
TATTTGTGGAACAGGGCAAG+AGG 0.600984 3_4:-500749 Msa0483750:intron
ACCAGAATTATGGGGACACC+AGG 0.601533 3_4:+500438 None:intergenic
ACTGTTGGTGATGGAGCTGT+TGG 0.601926 3_4:-501821 Msa0483750:CDS
GTATTTATTGCAGAACGTGA+AGG 0.606998 3_4:-499564 Msa0483750:intron
AGAAGAAAGTACTGGAGGAG+GGG 0.608480 3_4:-501926 Msa0483750:five_prime_UTR
GGTGTCCCCATAATTCTGGT+TGG 0.615899 3_4:-500435 Msa0483750:CDS
GAGTTGAAGCATTATGCACC+TGG 0.616427 3_4:-500456 Msa0483750:CDS
TCGGCACCACGGTAACTCAA+AGG 0.619029 3_4:+500709 None:intergenic
TATTAAAGGGAGAAGAGCTG+AGG 0.626553 3_4:-499714 Msa0483750:intron
TTGTTCCAACCAGAATTATG+GGG 0.627746 3_4:+500430 None:intergenic
CTGAGCTGTGGTAATGGGAA+CGG 0.629176 3_4:+499817 None:intergenic
TGTCCTCAGGTGAAGATCTG+AGG 0.631328 3_4:-501900 Msa0483750:five_prime_UTR
GAAAGCCAATATGAAAACAT+CGG 0.632883 3_4:+500690 None:intergenic
TTAAGACCTTTGAGTTACCG+TGG 0.650534 3_4:-500715 Msa0483750:CDS
AGAAGTTGGAGAGTCTGTGT+GGG 0.653335 3_4:-499367 Msa0483750:three_prime_UTR
TGTGTTAACAACGAAACGAA+CGG 0.653824 3_4:-502226 Msa0483750:five_prime_UTR
TGACAATTTCAGTGCGAATG+TGG 0.655180 3_4:-500892 Msa0483750:CDS
AGAACTGCTTATCATCCCGA+AGG 0.656406 3_4:+499858 None:intergenic
AAGAAGTTGGAGAGTCTGTG+TGG 0.657940 3_4:-499368 Msa0483750:three_prime_UTR
TAATGGGAACGGCACCAGGA+TGG 0.658560 3_4:+499828 None:intergenic
TTTATTGCAGAACGTGAAGG+CGG 0.659717 3_4:-499561 Msa0483750:intron
GATCTCCTTGAAGCATAAGG+TGG 0.664504 3_4:-499321 Msa0483750:three_prime_UTR
GGGGAATACAGGTAGAACAA+AGG 0.683373 3_4:+499423 None:intergenic
ATGCAGTTCAAAATCACAGG+AGG 0.690232 3_4:-499662 Msa0483750:intron
TAGCCTCAGATCTTCACCTG+AGG 0.705958 3_4:+501897 None:intergenic
ATATGAAAACATCGGCACCA+CGG 0.709782 3_4:+500698 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTCAAATTTTTATTTT+TGG - chr3_4:499789-499808 Msa0483750:intron 10.0%
!!! AATTGAAAATTTTGATTTTT+CGG - chr3_4:499853-499872 Msa0483750:CDS 10.0%
!! TAATGTCTTTAAATTAAAAA+AGG - chr3_4:500157-500176 Msa0483750:intron 10.0%
!!! TTATTTTATTTATATTTTGG+TGG - chr3_4:500688-500707 Msa0483750:CDS 10.0%
!! TTAATACCATATATAAATAT+GGG + chr3_4:501152-501171 None:intergenic 10.0%
!! ATTAATACCATATATAAATA+TGG + chr3_4:501153-501172 None:intergenic 10.0%
!! AAATTGAAGAAAAAAGAAAA+AGG + chr3_4:499767-499786 None:intergenic 15.0%
!!! TTTGGAATTTATATGTAATT+GGG - chr3_4:500267-500286 Msa0483750:intron 15.0%
!!! AGTTTGTTGAATTTAAAATT+TGG - chr3_4:500293-500312 Msa0483750:intron 15.0%
!!! GTTTGTTGAATTTAAAATTT+GGG - chr3_4:500294-500313 Msa0483750:intron 15.0%
!! AAATACATAAAAAAACACAA+GGG + chr3_4:500910-500929 None:intergenic 15.0%
!! AAAATACATAAAAAAACACA+AGG + chr3_4:500911-500930 None:intergenic 15.0%
!!! TTGTGTTTTTTTATGTATTT+TGG - chr3_4:500910-500929 Msa0483750:CDS 15.0%
!! TAATACCATATATAAATATG+GGG + chr3_4:501151-501170 None:intergenic 15.0%
!! ATTTATATATGGTATTAATC+AGG - chr3_4:501154-501173 Msa0483750:intron 15.0%
!! TTTATATATGGTATTAATCA+GGG - chr3_4:501155-501174 Msa0483750:intron 15.0%
!! GAAAATATTTAATACAAATC+TGG + chr3_4:501490-501509 None:intergenic 15.0%
!!! TTTATTAAATGTGTTACTGT+TGG - chr3_4:499665-499684 Msa0483750:CDS 20.0%
!!! AATTTTTATTTTTGGGTATC+TGG - chr3_4:499797-499816 Msa0483750:intron 20.0%
!!! ATTTTTATTTTTGGGTATCT+GGG - chr3_4:499798-499817 Msa0483750:intron 20.0%
!!! ATTCTTGTAAATTTGAAATC+TGG - chr3_4:499894-499913 Msa0483750:intron 20.0%
!! CAAATCATAATTTACATACT+CGG + chr3_4:499944-499963 None:intergenic 20.0%
!!! TAAATTATGATTTGGAATGA+TGG - chr3_4:499950-499969 Msa0483750:intron 20.0%
!!! AAATTATGATTTGGAATGAT+GGG - chr3_4:499951-499970 Msa0483750:intron 20.0%
!!! GTTTGGAATTTATATGTAAT+TGG - chr3_4:500266-500285 Msa0483750:intron 20.0%
!! AGTCATGTAAATTTCAAATT+TGG - chr3_4:500335-500354 Msa0483750:intron 20.0%
!! TGTAAATTTCAAATTTGGTA+TGG - chr3_4:500340-500359 Msa0483750:intron 20.0%
!! TAATTGTTGGAATTAAAAGT+TGG - chr3_4:500478-500497 Msa0483750:intron 20.0%
!! AATTGTTGGAATTAAAAGTT+GGG - chr3_4:500479-500498 Msa0483750:intron 20.0%
!!! ATATTGATTTGTGAATTTAC+TGG - chr3_4:500522-500541 Msa0483750:intron 20.0%
!! GGATTTATTATGTTTATTTG+TGG - chr3_4:500738-500757 Msa0483750:CDS 20.0%
!! AATATTAAGTCGAAAAAAAC+TGG + chr3_4:501587-501606 None:intergenic 20.0%
!! GATACAAATGACAAAATAAT+AGG + chr3_4:501615-501634 None:intergenic 20.0%
!! ATTAAATTAAATCTGACATG+TGG - chr3_4:501760-501779 Msa0483750:intron 20.0%
!! TTAAATTAAATCTGACATGT+GGG - chr3_4:501761-501780 Msa0483750:intron 20.0%
!! AAAGAAAATGATGAAAAGAA+GGG + chr3_4:502106-502125 None:intergenic 20.0%
! AATTGAGAAAATGCAGAATT+GGG + chr3_4:499423-499442 None:intergenic 25.0%
! TAATTGAGAAAATGCAGAAT+TGG + chr3_4:499424-499443 None:intergenic 25.0%
!! AATTCTGCATTTTCTCAATT+AGG - chr3_4:499423-499442 Msa0483750:three_prime_UTR 25.0%
!! ATTCTGCATTTTCTCAATTA+GGG - chr3_4:499424-499443 Msa0483750:three_prime_UTR 25.0%
! CGAGTATGTAAATTATGATT+TGG - chr3_4:499942-499961 Msa0483750:intron 25.0%
! ACATTTAGTGTTTGATGTAA+AGG - chr3_4:500412-500431 Msa0483750:intron 25.0%
!! ATGGATATAATGTGAGATTT+TGG - chr3_4:500440-500459 Msa0483750:CDS 25.0%
! TTGAGTTTATGTGTAATTGT+TGG - chr3_4:500465-500484 Msa0483750:CDS 25.0%
!!! TTTGGTGGATTTTTGTTATT+TGG - chr3_4:500703-500722 Msa0483750:CDS 25.0%
!!! TTGGTGGATTTTTGTTATTT+GGG - chr3_4:500704-500723 Msa0483750:CDS 25.0%
! ATTATGTTTATTTGTGGAAC+AGG - chr3_4:500744-500763 Msa0483750:intron 25.0%
! TTATGTTTATTTGTGGAACA+GGG - chr3_4:500745-500764 Msa0483750:intron 25.0%
! TTATGAAAATGTCTCCAAAA+AGG - chr3_4:500854-500873 Msa0483750:CDS 25.0%
!!! TATGTATTTTGGTTTATCTG+TGG - chr3_4:500921-500940 Msa0483750:intron 25.0%
! ATATATAAATATGGGGAAGA+TGG + chr3_4:501144-501163 None:intergenic 25.0%
! ATCTTCCCCATATTTATATA+TGG - chr3_4:501143-501162 Msa0483750:intron 25.0%
! TAGTTGATTATGAAATGCAT+GGG + chr3_4:501207-501226 None:intergenic 25.0%
! ATAGTTGATTATGAAATGCA+TGG + chr3_4:501208-501227 None:intergenic 25.0%
! AAAACTAAAACAAACCAGAA+AGG + chr3_4:501429-501448 None:intergenic 25.0%
!!! ATTTTTTATTCTGCTACTCT+AGG - chr3_4:501710-501729 Msa0483750:intron 25.0%
! TTGATTCTTCTAATTCACAA+TGG - chr3_4:501882-501901 Msa0483750:five_prime_UTR 25.0%
!! AATGTGAAGTTTAAGTTTGT+TGG - chr3_4:501912-501931 Msa0483750:five_prime_UTR 25.0%
! AAAGAAGAATAAAGCACAAA+AGG - chr3_4:502012-502031 Msa0483750:intron 25.0%
! AGTGAATTACAATATTGAAC+AGG + chr3_4:502038-502057 None:intergenic 25.0%
! GTTCAATATTGTAATTCACT+AGG - chr3_4:502038-502057 Msa0483750:intron 25.0%
! AAGAAAATGATGAAAAGAAG+GGG + chr3_4:502105-502124 None:intergenic 25.0%
! CAAAGAAAATGATGAAAAGA+AGG + chr3_4:502107-502126 None:intergenic 25.0%
!!! TTCTTTGTTATTGAAGAAGT+TGG - chr3_4:502120-502139 Msa0483750:intron 25.0%
AATCACAACTTCGTTCAAAA+AGG - chr3_4:499206-499225 Msa0483750:three_prime_UTR 30.0%
AAGAGGATATACGAAATAGT+TGG - chr3_4:499247-499266 Msa0483750:three_prime_UTR 30.0%
AGAAAGAGAAAGAACCTATT+TGG + chr3_4:499329-499348 None:intergenic 30.0%
GAAATTTGGGGAAAAAAAGA+AGG + chr3_4:499401-499420 None:intergenic 30.0%
AATGCAGAATTGGGAAATTT+GGG + chr3_4:499414-499433 None:intergenic 30.0%
AAATGCAGAATTGGGAAATT+TGG + chr3_4:499415-499434 None:intergenic 30.0%
! GAAGAATAAGAAGAAAGTAC+TGG - chr3_4:499567-499586 Msa0483750:intron 30.0%
AATTCAAAGTTGAAAAAGCC+AGG + chr3_4:499837-499856 None:intergenic 30.0%
!!! AATTTTGATTTTTCGGCGTT+TGG - chr3_4:499860-499879 Msa0483750:CDS 30.0%
! TGTAAATTTGAAATCTGGCA+TGG - chr3_4:499899-499918 Msa0483750:intron 30.0%
!! ATGGGTTGGATGTTAATTTA+AGG - chr3_4:499969-499988 Msa0483750:intron 30.0%
! TGGGTTGGATGTTAATTTAA+GGG - chr3_4:499970-499989 Msa0483750:intron 30.0%
! TGTTAATTTAAGGGTGTGTT+TGG - chr3_4:499979-499998 Msa0483750:intron 30.0%
! GTTAATTTAAGGGTGTGTTT+GGG - chr3_4:499980-499999 Msa0483750:intron 30.0%
TGTATCCATTTCAACCTTTA+GGG + chr3_4:500233-500252 None:intergenic 30.0%
TTGTATCCATTTCAACCTTT+AGG + chr3_4:500234-500253 None:intergenic 30.0%
GTTTGATGTAAAGGTTGAAA+TGG - chr3_4:500421-500440 Msa0483750:intron 30.0%
!! ATTTTGTCGAACTTGCTTTT+TGG - chr3_4:500556-500575 Msa0483750:intron 30.0%
CTGAAATTGTCAAAAACTGT+CGG + chr3_4:500601-500620 None:intergenic 30.0%
!! TAATGGAAGTATTGTGAATC+TGG - chr3_4:500636-500655 Msa0483750:intron 30.0%
!! AATGGAAGTATTGTGAATCT+GGG - chr3_4:500637-500656 Msa0483750:intron 30.0%
GAAAGCCAATATGAAAACAT+CGG + chr3_4:500814-500833 None:intergenic 30.0%
! TTTGGAGACATTTTCATAAC+TGG + chr3_4:500853-500872 None:intergenic 30.0%
TCTGTGGTGAACAATATATA+CGG - chr3_4:500937-500956 Msa0483750:intron 30.0%
TTTGTTCCAACCAGAATTAT+GGG + chr3_4:501075-501094 None:intergenic 30.0%
AGTTGATTATGAAATGCATG+GGG + chr3_4:501206-501225 None:intergenic 30.0%
AGATACCAGTAGAAAAACAA+TGG + chr3_4:501370-501389 None:intergenic 30.0%
CATACATTGTATCTGCAATT+GGG - chr3_4:501452-501471 Msa0483750:intron 30.0%
ATACATTGTATCTGCAATTG+GGG - chr3_4:501453-501472 Msa0483750:intron 30.0%
TTAATACAAATCTGGAGACA+AGG + chr3_4:501482-501501 None:intergenic 30.0%
! TTCTTCTTTTTCTTCTGCTT+TGG + chr3_4:502001-502020 None:intergenic 30.0%
TGTGTTAACAACGAAACGAA+CGG - chr3_4:499275-499294 Msa0483750:three_prime_UTR 35.0%
GGGAAAAAAAGAAGGAAATG+AGG + chr3_4:499393-499412 None:intergenic 35.0%
ATGCAGAATTGGGAAATTTG+GGG + chr3_4:499413-499432 None:intergenic 35.0%
!! GAATAAGAAGAAAGTACTGG+AGG - chr3_4:499570-499589 Msa0483750:intron 35.0%
!! TGAAGATCTGAGGCTATTTT+GGG - chr3_4:499611-499630 Msa0483750:intron 35.0%
!! TTTTCAAGATGAGTGCTTCT+AGG - chr3_4:499642-499661 Msa0483750:intron 35.0%
! AAATGTGTTACTGTTGGTGA+TGG - chr3_4:499671-499690 Msa0483750:CDS 35.0%
!! TATGATTTGGAATGATGGGT+TGG - chr3_4:499955-499974 Msa0483750:intron 35.0%
AGGTTGAAATGGATACAATG+TGG - chr3_4:500236-500255 Msa0483750:intron 35.0%
GGTTGAAATGGATACAATGT+GGG - chr3_4:500237-500256 Msa0483750:intron 35.0%
! ATGGATACAATGTGGGATTT+TGG - chr3_4:500244-500263 Msa0483750:intron 35.0%
!!! TACAATGTGGGATTTTGGTT+TGG - chr3_4:500249-500268 Msa0483750:intron 35.0%
!! GGTATGGAAAGTACTTTGTA+AGG - chr3_4:500356-500375 Msa0483750:intron 35.0%
! AAAGTACTTTGTAAGGCAAC+TGG - chr3_4:500363-500382 Msa0483750:intron 35.0%
AAGTACTTTGTAAGGCAACT+GGG - chr3_4:500364-500383 Msa0483750:intron 35.0%
TATTGTGAATCTGGGTTTGT+GGG - chr3_4:500645-500664 Msa0483750:intron 35.0%
!!! GTGGATTTTTGTTATTTGGG+AGG - chr3_4:500707-500726 Msa0483750:CDS 35.0%
! TTCTTGTTCCCATTTTGCTT+TGG - chr3_4:500881-500900 Msa0483750:CDS 35.0%
CTGTGGTGAACAATATATAC+GGG - chr3_4:500938-500957 Msa0483750:intron 35.0%
TTGTTCCAACCAGAATTATG+GGG + chr3_4:501074-501093 None:intergenic 35.0%
CTTTGTTCCAACCAGAATTA+TGG + chr3_4:501076-501095 None:intergenic 35.0%
!! CATCACCATTGTTTTTCTAC+TGG - chr3_4:501362-501381 Msa0483750:intron 35.0%
! GGTATCTGATACATCAGTTT+TGG - chr3_4:501383-501402 Msa0483750:intron 35.0%
!! TTTTGATGTTTCTGCCTTTC+TGG - chr3_4:501412-501431 Msa0483750:intron 35.0%
CCAATTGCAGATACAATGTA+TGG + chr3_4:501454-501473 None:intergenic 35.0%
CCATACATTGTATCTGCAAT+TGG - chr3_4:501451-501470 Msa0483750:intron 35.0%
AGCTAACAAAACTACTCGAT+GGG + chr3_4:501522-501541 None:intergenic 35.0%
AAGCTAACAAAACTACTCGA+TGG + chr3_4:501523-501542 None:intergenic 35.0%
TTAGCTTCCAGTTAGACAAA+TGG - chr3_4:501536-501555 Msa0483750:intron 35.0%
AAAAAACTGGTACACTGATC+AGG + chr3_4:501574-501593 None:intergenic 35.0%
TTTGTATCGAGATAGACCTT+CGG - chr3_4:501627-501646 Msa0483750:intron 35.0%
ATAAAAAATCACCTGAGCTG+TGG + chr3_4:501699-501718 None:intergenic 35.0%
TGAATGCAGTTCAAAATCAC+AGG - chr3_4:501836-501855 Msa0483750:CDS 35.0%
! TCAAAATCACAGGAGGTATA+TGG - chr3_4:501846-501865 Msa0483750:CDS 35.0%
GTATTTATTGCAGAACGTGA+AGG - chr3_4:501937-501956 Msa0483750:five_prime_UTR 35.0%
! TTCTTTTTCTTCTGCTTTGG+TGG + chr3_4:501998-502017 None:intergenic 35.0%
AAATGATGAAAAGAAGGGGA+GGG + chr3_4:502101-502120 None:intergenic 35.0%
AAAATGATGAAAAGAAGGGG+AGG + chr3_4:502102-502121 None:intergenic 35.0%
TAATTGCCTTCTTCTCTCAC+AGG - chr3_4:499357-499376 Msa0483750:three_prime_UTR 40.0%
AATTGCCTTCTTCTCTCACA+GGG - chr3_4:499358-499377 Msa0483750:three_prime_UTR 40.0%
!! TAAGAAGAAAGTACTGGAGG+AGG - chr3_4:499573-499592 Msa0483750:intron 40.0%
!! AAGAAGAAAGTACTGGAGGA+GGG - chr3_4:499574-499593 Msa0483750:intron 40.0%
!! GTGAAGATCTGAGGCTATTT+TGG - chr3_4:499610-499629 Msa0483750:intron 40.0%
CTGGGTTGTGTTTAAATTCC+TGG - chr3_4:499816-499835 Msa0483750:intron 40.0%
GGCATGGAAAATGCTTTGTA+AGG - chr3_4:499915-499934 Msa0483750:intron 40.0%
!! TCAACCTTTAGGGTGTGTTT+GGG + chr3_4:500223-500242 None:intergenic 40.0%
!! TTCAACCTTTAGGGTGTGTT+TGG + chr3_4:500224-500243 None:intergenic 40.0%
ACACACCCTAAAGGTTGAAA+TGG - chr3_4:500225-500244 Msa0483750:intron 40.0%
!!! TGTCGAACTTGCTTTTTGGT+TGG - chr3_4:500560-500579 Msa0483750:intron 40.0%
!!! CTTGCTTTTTGGTTGGAAAC+AGG - chr3_4:500567-500586 Msa0483750:intron 40.0%
TGACAATTTCAGTGCGAATG+TGG - chr3_4:500609-500628 Msa0483750:intron 40.0%
AGTGCGAATGTGGTTGTTAA+TGG - chr3_4:500619-500638 Msa0483750:intron 40.0%
GTATTGTGAATCTGGGTTTG+TGG - chr3_4:500644-500663 Msa0483750:intron 40.0%
GTTATTTGGGAGGTTCTTTC+TGG - chr3_4:500717-500736 Msa0483750:CDS 40.0%
! TTAAGACCTTTGAGTTACCG+TGG - chr3_4:500786-500805 Msa0483750:intron 40.0%
ATATGAAAACATCGGCACCA+CGG + chr3_4:500806-500825 None:intergenic 40.0%
!!! TGGTGCCGATGTTTTCATAT+TGG - chr3_4:500806-500825 Msa0483750:intron 40.0%
!!! GGAACAAGAAAGCACCTTTT+TGG + chr3_4:500871-500890 None:intergenic 40.0%
AAGGGACACCAAAGCAAAAT+GGG + chr3_4:500892-500911 None:intergenic 40.0%
! CGTGCTTGTTTCATTTTCAG+TGG - chr3_4:501016-501035 Msa0483750:intron 40.0%
! ATTCTGGTTGGAACAAAGCT+TGG - chr3_4:501078-501097 Msa0483750:intron 40.0%
GAAGTTTCCATTTGTCGAAC+TGG - chr3_4:501250-501269 Msa0483750:intron 40.0%
CCTAGCAAACAAAAAGAACC+CGG + chr3_4:501288-501307 None:intergenic 40.0%
!! CGGGTTCTTTTTGTTTGCTA+GGG - chr3_4:501286-501305 Msa0483750:intron 40.0%
!! GGGTTCTTTTTGTTTGCTAG+GGG - chr3_4:501287-501306 Msa0483750:intron 40.0%
!! GGTTCTTTTTGTTTGCTAGG+GGG - chr3_4:501288-501307 Msa0483750:intron 40.0%
!! TTTTTGTTTGCTAGGGGGAA+GGG - chr3_4:501293-501312 Msa0483750:intron 40.0%
AGCACAACCATTTGTCTAAC+TGG + chr3_4:501546-501565 None:intergenic 40.0%
TTGTATCGAGATAGACCTTC+GGG - chr3_4:501628-501647 Msa0483750:intron 40.0%
TGACATGTGGGTCCTATTAA+AGG - chr3_4:501773-501792 Msa0483750:intron 40.0%
GACATGTGGGTCCTATTAAA+GGG - chr3_4:501774-501793 Msa0483750:intron 40.0%
TCAGCTCTTCTCCCTTTAAT+AGG + chr3_4:501788-501807 None:intergenic 40.0%
TATTAAAGGGAGAAGAGCTG+AGG - chr3_4:501787-501806 Msa0483750:CDS 40.0%
GAACTGCATTCAATGTAAGC+TGG + chr3_4:501828-501847 None:intergenic 40.0%
ATGCAGTTCAAAATCACAGG+AGG - chr3_4:501839-501858 Msa0483750:CDS 40.0%
TTTATTGCAGAACGTGAAGG+CGG - chr3_4:501940-501959 Msa0483750:five_prime_UTR 40.0%
TGGTTGAAGGACAACTCTTA+TGG + chr3_4:501978-501997 None:intergenic 40.0%
AATGATGAAAAGAAGGGGAG+GGG + chr3_4:502100-502119 None:intergenic 40.0%
CTAGATCTCCTTGAAGCATA+AGG - chr3_4:502177-502196 Msa0483750:five_prime_UTR 40.0%
TTAAGCCACCTTATGCTTCA+AGG + chr3_4:502188-502207 None:intergenic 40.0%
CACACTCTCAGCTTCCAAAT+AGG - chr3_4:499312-499331 Msa0483750:three_prime_UTR 45.0%
CTTCTTCTCTCACAGGGTTA+GGG - chr3_4:499364-499383 Msa0483750:three_prime_UTR 45.0%
!! AGAAGAAAGTACTGGAGGAG+GGG - chr3_4:499575-499594 Msa0483750:intron 45.0%
GAGAAGAATGACAAACCGTG+GGG + chr3_4:499745-499764 None:intergenic 45.0%
GGAGAAGAATGACAAACCGT+GGG + chr3_4:499746-499765 None:intergenic 45.0%
AGGAGAAGAATGACAAACCG+TGG + chr3_4:499747-499766 None:intergenic 45.0%
! TTAAGGGTGTGTTTGGGAAG+AGG - chr3_4:499986-500005 Msa0483750:intron 45.0%
! TAAGGGTGTGTTTGGGAAGA+GGG - chr3_4:499987-500006 Msa0483750:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+AGG - chr3_4:499993-500012 Msa0483750:intron 45.0%
TTTGGGAAGAGGGTTTAGGA+GGG - chr3_4:499997-500016 Msa0483750:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+GGG + chr3_4:499993-500012 None:intergenic 45.0%
TTAGGGTGTGTTTGGGAAGA+GGG + chr3_4:500216-500235 None:intergenic 45.0%
! TTTAGGGTGTGTTTGGGAAG+AGG + chr3_4:500217-500236 None:intergenic 45.0%
TCTTCCCAAACACACCCTAA+AGG - chr3_4:500216-500235 Msa0483750:intron 45.0%
TTGTGGGATACTGCTGGTAA+TGG - chr3_4:500661-500680 Msa0483750:CDS 45.0%
TATTTGTGGAACAGGGCAAG+AGG - chr3_4:500752-500771 Msa0483750:intron 45.0%
CAAGGGACACCAAAGCAAAA+TGG + chr3_4:500893-500912 None:intergenic 45.0%
GGTGCATAATGCTTCAACTC+TGG + chr3_4:501045-501064 None:intergenic 45.0%
! GAGTTGAAGCATTATGCACC+TGG - chr3_4:501045-501064 Msa0483750:intron 45.0%
CAGTTAGCCAGTTCGACAAA+TGG + chr3_4:501260-501279 None:intergenic 45.0%
!! CCGGGTTCTTTTTGTTTGCT+AGG - chr3_4:501285-501304 Msa0483750:intron 45.0%
!! CTTTTTGTTTGCTAGGGGGA+AGG - chr3_4:501292-501311 Msa0483750:intron 45.0%
!! TTTTGTTTGCTAGGGGGAAG+GGG - chr3_4:501294-501313 Msa0483750:intron 45.0%
AGAACTGCTTATCATCCCGA+AGG + chr3_4:501646-501665 None:intergenic 45.0%
ATCACCTGAGCTGTGGTAAT+GGG + chr3_4:501692-501711 None:intergenic 45.0%
AATCACCTGAGCTGTGGTAA+TGG + chr3_4:501693-501712 None:intergenic 45.0%
! TCTTCTGCTTTGGTGGTTGA+AGG + chr3_4:501991-502010 None:intergenic 45.0%
GGGGAATACAGGTAGAACAA+AGG + chr3_4:502081-502100 None:intergenic 45.0%
AAGAAGTTGGAGAGTCTGTG+TGG - chr3_4:502133-502152 Msa0483750:intron 45.0%
AGAAGTTGGAGAGTCTGTGT+GGG - chr3_4:502134-502153 Msa0483750:intron 45.0%
GATCTCCTTGAAGCATAAGG+TGG - chr3_4:502180-502199 Msa0483750:five_prime_UTR 45.0%
!! AATAAAAAAATAAAAATAAG+AGG - chr3_4:499230-499249 Msa0483750:three_prime_UTR 5.0%
!!! AATTTCAAATTTTTATTTTT+GGG - chr3_4:499790-499809 Msa0483750:intron 5.0%
!!! TTGTTATTTTATTTATATTT+TGG - chr3_4:500685-500704 Msa0483750:CDS 5.0%
!! ATAAAAACTTAAAAAAATAT+GGG + chr3_4:502272-502291 None:intergenic 5.0%
!! AATAAAAACTTAAAAAAATA+TGG + chr3_4:502273-502292 None:intergenic 5.0%
CCTAACCCTGTGAGAGAAGA+AGG + chr3_4:499366-499385 None:intergenic 50.0%
CCTTCTTCTCTCACAGGGTT+AGG - chr3_4:499363-499382 Msa0483750:three_prime_UTR 50.0%
TGTCCTCAGGTGAAGATCTG+AGG - chr3_4:499601-499620 Msa0483750:intron 50.0%
TAGCCTCAGATCTTCACCTG+AGG + chr3_4:499607-499626 None:intergenic 50.0%
!! ACTGTTGGTGATGGAGCTGT+TGG - chr3_4:499680-499699 Msa0483750:CDS 50.0%
CACCAGCAATACTTTCCCCA+CGG - chr3_4:499727-499746 Msa0483750:intron 50.0%
!! AACCGTGGGGAAAGTATTGC+TGG + chr3_4:499732-499751 None:intergenic 50.0%
GTTTGGGAAGAGGGTTTAGG+AGG - chr3_4:499996-500015 Msa0483750:intron 50.0%
TTGGGAAGAGGGTTTAGGAG+GGG - chr3_4:499998-500017 Msa0483750:intron 50.0%
AAGAGGGTTTAGGAGGGGAA+GGG - chr3_4:500003-500022 Msa0483750:intron 50.0%
AAGAGGGTTTGGGAAGGGAA+GGG + chr3_4:500200-500219 None:intergenic 50.0%
TTGGGAAGAGGGTTTGGGAA+GGG + chr3_4:500205-500224 None:intergenic 50.0%
TTTGGGAAGAGGGTTTGGGA+AGG + chr3_4:500206-500225 None:intergenic 50.0%
GTGTGTTTGGGAAGAGGGTT+TGG + chr3_4:499992-500011 None:intergenic 50.0%
ACCTGGTGTCCCCATAATTC+TGG - chr3_4:501062-501081 Msa0483750:intron 50.0%
ACCAGAATTATGGGGACACC+AGG + chr3_4:501066-501085 None:intergenic 50.0%
GGTGTCCCCATAATTCTGGT+TGG - chr3_4:501066-501085 Msa0483750:intron 50.0%
GAACTGGCTAACTGCTGAAC+CGG - chr3_4:501266-501285 Msa0483750:intron 50.0%
AACTGGCTAACTGCTGAACC+GGG - chr3_4:501267-501286 Msa0483750:intron 50.0%
! TTTGTTTGCTAGGGGGAAGG+GGG - chr3_4:501295-501314 Msa0483750:intron 50.0%
CAGTTCTGCATAGACCATCC+TGG - chr3_4:501659-501678 Msa0483750:intron 50.0%
!! CTGAGCTGTGGTAATGGGAA+CGG + chr3_4:501687-501706 None:intergenic 50.0%
AAAGAAGGGGAGGGGAATAC+AGG + chr3_4:502092-502111 None:intergenic 50.0%
GAAGAGGGTTTAGGAGGGGA+AGG - chr3_4:500002-500021 Msa0483750:intron 55.0%
GAAGAGGGTTTGGGAAGGGA+AGG + chr3_4:500201-500220 None:intergenic 55.0%
CTGGGTTTGTGGGATACTGC+TGG - chr3_4:500655-500674 Msa0483750:CDS 55.0%
TCGGCACCACGGTAACTCAA+AGG + chr3_4:500795-500814 None:intergenic 55.0%
! TTGTTTGCTAGGGGGAAGGG+GGG - chr3_4:501296-501315 Msa0483750:intron 55.0%
TAATGGGAACGGCACCAGGA+TGG + chr3_4:501676-501695 None:intergenic 55.0%
CGTTCCCATTACCACAGCTC+AGG - chr3_4:501685-501704 Msa0483750:intron 55.0%
AGGGTTTAGGAGGGGAAGGG+AGG - chr3_4:500006-500025 Msa0483750:intron 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG - chr3_4:500007-500026 Msa0483750:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG - chr3_4:500008-500027 Msa0483750:intron 60.0%
GGTTTGGGAAGGGAAGGGAG+GGG + chr3_4:500195-500214 None:intergenic 60.0%
GGGTTTGGGAAGGGAAGGGA+GGG + chr3_4:500196-500215 None:intergenic 60.0%
AGGGTTTGGGAAGGGAAGGG+AGG + chr3_4:500197-500216 None:intergenic 60.0%
TAGGGGGAAGGGGGGATTTC+TGG - chr3_4:501304-501323 Msa0483750:intron 60.0%
!! GTGGTAATGGGAACGGCACC+AGG + chr3_4:501680-501699 None:intergenic 60.0%
GGAGGAGGGGAACTGTCCTC+AGG - chr3_4:499588-499607 Msa0483750:intron 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG - chr3_4:500011-500030 Msa0483750:intron 65.0%
TAGGAGGGGAAGGGAGGGGA+GGG - chr3_4:500012-500031 Msa0483750:intron 65.0%
TGGGAAGGGAAGGGAGGGGA+GGG + chr3_4:500191-500210 None:intergenic 65.0%
TTGGGAAGGGAAGGGAGGGG+AGG + chr3_4:500192-500211 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 499174 502349 499174 ID=Msa0483750;Name=Msa0483750
chr3_4 mRNA 499174 502349 499174 ID=Msa0483750-mRNA-1;Parent=Msa0483750;Name=Msa0483750-mRNA-1;_AED=0.08;_eAED=0.08;_QI=314|1|1|1|0.85|0.75|8|298|197
chr3_4 exon 499174 499573 499174 ID=Msa0483750-mRNA-1:exon:188;Parent=Msa0483750-mRNA-1
chr3_4 exon 499663 499728 499663 ID=Msa0483750-mRNA-1:exon:187;Parent=Msa0483750-mRNA-1
chr3_4 exon 499817 499881 499817 ID=Msa0483750-mRNA-1:exon:186;Parent=Msa0483750-mRNA-1
chr3_4 exon 500424 500487 500424 ID=Msa0483750-mRNA-1:exon:185;Parent=Msa0483750-mRNA-1
chr3_4 exon 500648 500757 500648 ID=Msa0483750-mRNA-1:exon:184;Parent=Msa0483750-mRNA-1
chr3_4 exon 500847 500934 500847 ID=Msa0483750-mRNA-1:exon:183;Parent=Msa0483750-mRNA-1
chr3_4 exon 501775 501981 501775 ID=Msa0483750-mRNA-1:exon:182;Parent=Msa0483750-mRNA-1
chr3_4 exon 502144 502349 502144 ID=Msa0483750-mRNA-1:exon:181;Parent=Msa0483750-mRNA-1
chr3_4 five_prime_UTR 502144 502349 502144 ID=Msa0483750-mRNA-1:five_prime_utr;Parent=Msa0483750-mRNA-1
chr3_4 five_prime_UTR 501874 501981 501874 ID=Msa0483750-mRNA-1:five_prime_utr;Parent=Msa0483750-mRNA-1
chr3_4 CDS 501775 501873 501775 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 500847 500934 500847 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 500648 500757 500648 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 500424 500487 500424 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 499817 499881 499817 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 499663 499728 499663 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 CDS 499472 499573 499472 ID=Msa0483750-mRNA-1:cds;Parent=Msa0483750-mRNA-1
chr3_4 three_prime_UTR 499174 499471 499174 ID=Msa0483750-mRNA-1:three_prime_utr;Parent=Msa0483750-mRNA-1
Gene Sequence

>Msa0483750

ATGAGTGCTTCTAGGTTTATTAAATGTGTTACTGTTGGTGATGGAGCTGTTGGAAAAACTTGTTTGTTGATTTCTTACACCAGCAATACTTTCCCCACGGATTATGTGCCGACAGTTTTTGACAATTTCAGTGCGAATGTGGTTGTTAATGGAAGTATTGTGAATCTGGGTTTGTGGGATACTGCTGGGCAAGAGGATTATAACAGATTAAGACCTTTGAGTTACCGTGGTGCCGATGTTTTCATATTGGCTTTCTCTCTCATAAGCAAAGCCAGTTATGAAAATGTCTCCAAAAAGTGGATTCCAGAGTTGAAGCATTATGCACCTGGTGTCCCCATAATTCTGGTTGGAACAAAGCTTGACCTTCGGGATGATAAGCAGTTCTGCATAGACCATCCTGGTGCCGTTCCCATTACCACAGCTCAGGGAGAAGAGCTGAGGAAGCTGATTAATGCACCAGCTTACATTGAATGCAGTTCAAAATCACAGGAGAACGTGAAGGCGGTGTTTGACGCAGCCATAAGAGTTGTCCTTCAACCACCAAAGCAGAAGAAAAAGAAGAATAAAGCACAAAAGGCCTGTTCAATATTGTAA
Protein sequence

>Msa0483750

MSASRFIKCVTVGDGAVGKTCLLISYTSNTFPTDYVPTVFDNFSANVVVNGSIVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIILVGTKLDLRDDKQFCIDHPGAVPITTAQGEELRKLINAPAYIECSSKSQENVKAVFDAAIRVVLQPPKQKKKKNKAQKACSIL*