AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0838290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838290 MtrunA17_Chr5g0405791 100.000 197 0 0 1 197 1 197 2.40e-147 407
Msa0838290 MtrunA17_Chr8g0371501 97.462 197 5 0 1 197 1 197 1.10e-143 397
Msa0838290 MtrunA17_Chr4g0037021 89.340 197 21 0 1 197 1 197 1.93e-133 372
Msa0838290 MtrunA17_Chr3g0117931 77.835 194 42 1 2 194 4 197 1.41e-116 330
Msa0838290 MtrunA17_Chr2g0325101 84.270 178 28 0 1 178 1 178 8.01e-116 327
Msa0838290 MtrunA17_Chr6g0486041 74.874 199 47 1 1 196 1 199 1.40e-111 317
Msa0838290 MtrunA17_Chr4g0046211 88.112 143 17 0 1 143 1 143 5.57e-94 270
Msa0838290 MtrunA17_Chr7g0265001 30.851 188 116 5 8 192 18 194 1.09e-25 98.6
Msa0838290 MtrunA17_Chr1g0182321 31.383 188 116 6 6 191 15 191 7.56e-25 96.7
Msa0838290 MtrunA17_Chr1g0182301 31.383 188 116 6 6 191 15 191 1.31e-24 95.9
Msa0838290 MtrunA17_Chr1g0212521 32.386 176 106 6 6 179 15 179 1.60e-24 95.9
Msa0838290 MtrunA17_Chr4g0030911 32.386 176 106 6 6 179 15 179 2.78e-24 95.1
Msa0838290 MtrunA17_Chr7g0263071 30.851 188 117 6 6 191 15 191 3.86e-24 94.7
Msa0838290 MtrunA17_Chr8g0384311 30.726 179 109 5 6 181 13 179 2.64e-23 92.4
Msa0838290 MtrunA17_Chr3g0130271 29.630 189 119 5 7 192 13 190 3.95e-23 92.0
Msa0838290 MtrunA17_Chr1g0199331 31.461 178 105 7 7 180 32 196 8.76e-23 90.9
Msa0838290 MtrunA17_Chr1g0180291 30.899 178 108 5 7 181 15 180 1.44e-22 90.5
Msa0838290 MtrunA17_Chr8g0349711 30.814 172 105 5 1 170 1 160 2.85e-22 89.7
Msa0838290 MtrunA17_Chr8g0335471 33.516 182 102 8 6 180 8 177 4.21e-22 89.0
Msa0838290 MtrunA17_Chr1g0159481 30.726 179 109 5 6 181 17 183 4.99e-22 89.4
Msa0838290 MtrunA17_Chr7g0275991 33.939 165 95 5 8 170 22 174 5.37e-22 89.4
Msa0838290 MtrunA17_Chr4g0055651 29.947 187 112 6 6 185 8 182 6.03e-22 88.6
Msa0838290 MtrunA17_Chr8g0390561 31.111 180 109 6 8 184 11 178 1.42e-21 87.4
Msa0838290 MtrunA17_Chr7g0250531 31.016 187 110 7 6 185 8 182 1.53e-21 87.4
Msa0838290 MtrunA17_Chr4g0055081 32.727 165 97 5 8 170 11 163 2.05e-21 87.4
Msa0838290 MtrunA17_Chr2g0276931 30.857 175 104 6 1 170 1 163 2.79e-21 87.0
Msa0838290 MtrunA17_Chr3g0109721 32.961 179 105 5 8 183 12 178 8.98e-21 85.5
Msa0838290 MtrunA17_Chr2g0319951 29.282 181 114 5 6 184 13 181 1.01e-20 85.9
Msa0838290 MtrunA17_Chr5g0400131 32.727 165 97 5 8 170 11 163 1.97e-20 84.7
Msa0838290 MtrunA17_Chr2g0327911 29.891 184 114 6 6 185 15 187 2.55e-20 84.7
Msa0838290 MtrunA17_Chr8g0346421 38.655 119 70 3 8 124 28 145 2.68e-20 85.1
Msa0838290 MtrunA17_Chr6g0458081 27.624 181 117 5 1 179 1 169 2.72e-20 84.3
Msa0838290 MtrunA17_Chr2g0290891 27.907 172 110 5 1 170 1 160 3.85e-20 84.0
Msa0838290 MtrunA17_Chr8g0349681 30.233 172 106 5 1 170 8 167 8.83e-20 86.3
Msa0838290 MtrunA17_Chr8g0349681 29.651 172 107 5 1 170 379 538 5.21e-19 84.3
Msa0838290 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 170 329 1.22e-18 83.2
Msa0838290 MtrunA17_Chr5g0433561 31.515 165 99 4 8 170 12 164 2.27e-19 81.6
Msa0838290 MtrunA17_Chr4g0040781 35.606 132 82 3 6 135 13 143 3.82e-19 81.6
Msa0838290 MtrunA17_Chr1g0201121 30.729 192 116 7 1 184 1 183 4.18e-19 81.3
Msa0838290 MtrunA17_Chr3g0123971 30.909 165 100 4 8 170 11 163 4.46e-19 81.3
Msa0838290 MtrunA17_Chr4g0046261 29.834 181 113 5 6 184 13 181 4.61e-19 81.3
Msa0838290 MtrunA17_Chr2g0276941 30.994 171 104 5 6 174 15 173 4.87e-19 81.3
Msa0838290 MtrunA17_Chr3g0112681 29.834 181 113 5 6 184 12 180 5.46e-19 81.3
Msa0838290 MtrunA17_Chr2g0315551 35.833 120 74 3 6 123 13 131 5.74e-19 81.3
Msa0838290 MtrunA17_Chr1g0174281 32.530 166 98 5 7 170 16 169 6.21e-19 81.3
Msa0838290 MtrunA17_Chr8g0382221 31.515 165 99 5 8 170 11 163 6.56e-19 80.9
Msa0838290 MtrunA17_Chr2g0283131 35.833 120 74 3 6 123 13 131 1.05e-18 80.5
Msa0838290 MtrunA17_Chr1g0197331 28.804 184 116 5 7 186 16 188 1.31e-18 80.1
Msa0838290 MtrunA17_Chr7g0234201 28.947 190 121 5 6 193 13 190 1.69e-18 79.7
Msa0838290 MtrunA17_Chr7g0231131 31.928 166 99 6 7 170 16 169 2.65e-18 79.3
Msa0838290 MtrunA17_Chr8g0381841 31.515 165 99 5 8 170 19 171 6.75e-18 78.6
Msa0838290 MtrunA17_Chr8g0392931 35.294 119 74 3 6 122 14 131 1.19e-17 77.8
Msa0838290 MtrunA17_Chr2g0331691 29.032 186 117 6 6 185 10 186 1.38e-17 77.4
Msa0838290 MtrunA17_Chr7g0214241 28.947 190 121 5 6 193 15 192 1.53e-17 77.4
Msa0838290 MtrunA17_Chr4g0034871 27.957 186 119 5 6 185 8 184 2.64e-17 76.6
Msa0838290 MtrunA17_Chr3g0123821 31.325 166 98 6 8 170 18 170 3.04e-17 76.6
Msa0838290 MtrunA17_Chr6g0466301 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0838290 MtrunA17_Chr6g0465491 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0838290 MtrunA17_Chr4g0032201 29.341 167 104 5 6 170 12 166 5.92e-17 75.9
Msa0838290 MtrunA17_Chr1g0189361 30.114 176 105 6 6 177 14 175 6.15e-17 75.9
Msa0838290 MtrunA17_Chr8g0351861 31.138 167 101 6 6 170 16 170 6.27e-17 75.9
Msa0838290 MtrunA17_Chr2g0325091 36.667 120 73 3 6 123 11 129 1.74e-16 74.3
Msa0838290 MtrunA17_Chr4g0004661 28.497 193 121 7 1 185 57 240 2.52e-16 74.7
Msa0838290 MtrunA17_Chr8g0383261 28.235 170 109 3 6 170 12 173 2.58e-16 73.9
Msa0838290 MtrunA17_Chr6g0482721 33.065 124 80 3 6 127 12 134 9.61e-16 72.4
Msa0838290 MtrunA17_Chr7g0259771 28.877 187 99 4 7 180 9 174 4.67e-15 72.8
Msa0838290 MtrunA17_Chr3g0138121 28.485 165 103 4 7 170 14 164 6.94e-14 67.4
Msa0838290 MtrunA17_Chr3g0138101 28.485 165 103 4 7 170 14 164 7.16e-14 67.4
Msa0838290 MtrunA17_Chr3g0138111 28.485 165 103 4 7 170 14 164 8.00e-14 67.4
Msa0838290 MtrunA17_Chr1g0185591 27.604 192 95 6 7 180 13 178 8.38e-14 69.3
Msa0838290 MtrunA17_Chr1g0191811 28.571 175 115 4 7 180 17 182 8.40e-13 66.2
Msa0838290 MtrunA17_Chr4g0046221 70.270 37 11 0 142 178 10 46 2.23e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0838290 AT2G17800.2 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0838290 AT2G17800.1 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0838290 AT3G51300.1 95.431 197 9 0 1 197 1 197 1.40e-127 357
Msa0838290 AT4G35950.1 95.939 197 8 0 1 197 1 197 1.42e-127 357
Msa0838290 AT4G35020.1 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0838290 AT4G35020.2 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0838290 AT4G35020.3 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0838290 AT1G75840.1 91.573 178 15 0 1 178 1 178 5.36e-122 343
Msa0838290 AT1G20090.1 90.341 176 17 0 3 178 2 177 3.09e-119 336
Msa0838290 AT5G45970.1 86.034 179 25 0 1 179 1 179 1.71e-117 332
Msa0838290 AT4G28950.1 75.500 200 43 2 1 196 1 198 2.63e-111 317
Msa0838290 AT5G62880.1 80.447 179 35 0 2 180 4 182 8.69e-111 315
Msa0838290 AT3G48040.1 80.447 179 35 0 2 180 4 182 8.96e-110 313
Msa0838290 AT2G44690.1 75.000 192 48 0 6 197 18 209 2.40e-106 304
Msa0838290 AT5G62880.2 79.070 172 32 1 2 173 4 171 5.27e-103 295
Msa0838290 AT3G48040.2 80.368 163 32 0 2 164 4 166 4.32e-100 287
Msa0838290 AT3G51290.2 95.122 164 8 0 34 197 635 798 1.77e-96 297
Msa0838290 AT4G35020.4 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0838290 AT4G35020.5 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0838290 AT3G46060.2 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0838290 AT3G46060.3 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0838290 AT3G46060.1 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0838290 AT3G54840.2 33.333 168 97 6 7 171 34 189 8.90e-24 93.6
Msa0838290 AT3G54840.1 32.143 168 99 5 7 171 34 189 9.53e-24 93.6
Msa0838290 AT3G54840.3 31.928 166 98 5 9 171 2 155 3.62e-23 91.3
Msa0838290 AT1G02130.1 32.544 169 100 6 6 172 8 164 8.47e-23 91.3
Msa0838290 AT3G53610.2 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0838290 AT3G53610.3 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0838290 AT3G53610.1 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0838290 AT5G45130.1 33.333 165 96 4 8 170 12 164 1.10e-22 90.9
Msa0838290 AT5G59840.1 31.737 167 101 5 6 170 15 170 1.43e-22 90.9
Msa0838290 AT4G19640.1 33.333 165 96 4 8 170 12 164 2.49e-22 90.1
Msa0838290 AT1G43890.3 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0838290 AT1G43890.1 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0838290 AT1G43890.2 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0838290 AT1G16920.1 32.418 182 109 6 6 185 13 182 2.99e-22 90.1
Msa0838290 AT5G45130.2 33.129 163 95 4 10 170 22 172 5.58e-22 89.4
Msa0838290 AT1G73640.1 32.984 191 110 6 6 190 13 191 1.97e-21 88.6
Msa0838290 AT2G44610.1 33.333 165 96 5 8 170 11 163 2.27e-21 87.8
Msa0838290 AT5G03530.2 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0838290 AT5G03530.1 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0838290 AT4G18430.1 32.597 181 108 5 6 184 13 181 4.12e-21 87.0
Msa0838290 AT4G35860.1 27.624 181 117 5 1 179 1 169 1.11e-20 85.9
Msa0838290 AT4G17530.1 31.016 187 110 7 6 185 8 182 1.79e-20 85.1
Msa0838290 AT4G39890.1 34.731 167 92 6 8 170 11 164 1.82e-20 85.5
Msa0838290 AT5G47200.1 31.319 182 106 7 6 180 8 177 3.03e-20 84.7
Msa0838290 AT3G11730.1 29.940 167 103 5 6 170 8 162 3.37e-20 84.3
Msa0838290 AT2G22290.1 33.333 165 96 5 8 170 11 163 3.58e-20 84.3
Msa0838290 AT1G06400.1 32.335 167 99 5 6 170 13 167 6.72e-20 84.0
Msa0838290 AT4G19640.2 36.885 122 74 3 8 127 12 132 8.89e-20 82.0
Msa0838290 AT4G18800.1 36.667 120 73 3 6 123 13 131 9.53e-20 83.6
Msa0838290 AT1G09630.1 31.492 181 110 5 6 184 12 180 9.58e-20 83.6
Msa0838290 AT3G15060.1 31.285 179 109 5 8 184 15 181 1.65e-19 82.8
Msa0838290 AT4G17170.1 27.326 172 111 5 1 170 1 160 2.17e-19 82.4
Msa0838290 AT4G19640.3 34.074 135 86 3 8 140 12 145 2.68e-19 82.0
Msa0838290 AT2G31680.1 29.670 182 114 4 6 185 12 181 2.75e-19 82.4
Msa0838290 AT3G09900.1 32.934 167 99 6 6 170 15 170 3.29e-19 82.0
Msa0838290 AT3G07410.1 30.526 190 118 5 6 193 12 189 3.29e-19 82.0
Msa0838290 AT4G17160.1 26.744 172 112 5 1 170 1 160 3.69e-19 81.6
Msa0838290 AT1G18200.1 30.387 181 112 6 6 184 13 181 5.73e-19 81.6
Msa0838290 AT5G60860.1 30.387 181 112 5 6 184 13 181 5.85e-19 81.6
Msa0838290 AT2G43130.1 29.121 182 115 5 6 185 12 181 5.89e-19 81.3
Msa0838290 AT3G12160.1 32.530 166 98 5 7 170 16 169 5.91e-19 81.6
Msa0838290 AT5G45750.1 35.833 120 74 3 6 123 13 131 6.31e-19 81.3
Msa0838290 AT3G18820.1 29.570 186 116 6 6 185 8 184 7.28e-19 80.9
Msa0838290 AT5G03520.1 33.533 167 98 6 6 170 15 170 8.63e-19 80.9
Msa0838290 AT1G01200.1 34.132 167 95 6 7 170 29 183 1.66e-18 80.9
Msa0838290 AT1G28550.1 37.500 120 72 3 6 123 13 131 1.71e-18 80.1
Msa0838290 AT5G10260.1 32.121 165 98 5 8 170 11 163 2.11e-18 79.7
Msa0838290 AT1G05810.2 30.994 171 104 5 6 174 58 216 2.97e-18 80.5
Msa0838290 AT1G05810.1 29.670 182 114 5 6 185 12 181 3.26e-18 79.7
Msa0838290 AT2G33870.1 37.815 119 71 3 6 122 13 130 5.00e-18 79.0
Msa0838290 AT5G64990.1 33.333 165 96 5 8 170 9 161 7.57e-18 78.2
Msa0838290 AT5G47520.1 28.652 178 113 5 2 177 10 175 1.85e-17 77.4
Msa0838290 AT1G22740.1 29.167 192 119 6 1 184 1 183 2.47e-17 77.0
Msa0838290 AT1G52280.1 30.726 179 107 7 1 171 1 170 5.25e-17 76.3
Msa0838290 AT2G21880.1 27.174 184 121 4 6 184 9 184 9.54e-17 75.5
Msa0838290 AT4G39990.1 32.787 122 79 3 7 126 18 138 1.10e-16 75.5
Msa0838290 AT1G07410.1 34.454 119 75 3 6 122 12 129 1.27e-16 75.1
Msa0838290 AT5G59150.1 29.240 171 107 6 6 174 12 170 1.57e-16 75.1
Msa0838290 AT3G16100.1 29.947 187 114 7 1 179 1 178 1.87e-16 74.7
Msa0838290 AT5G47960.1 33.051 118 76 3 7 122 16 132 2.16e-16 74.7
Msa0838290 AT5G65270.1 28.729 181 113 6 7 184 18 185 2.31e-16 74.7
Msa0838290 AT4G09720.1 30.409 171 104 6 6 170 8 169 5.43e-16 73.2
Msa0838290 AT3G09910.3 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0838290 AT3G09910.1 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0838290 AT3G09910.2 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0838290 AT5G27540.1 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0838290 AT5G27540.2 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0838290 AT3G46830.1 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0838290 AT3G46830.2 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0838290 AT1G49300.2 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0838290 AT1G49300.1 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0838290 AT5G64990.2 31.977 172 96 6 8 170 9 168 3.92e-15 71.2
Msa0838290 AT5G55080.1 30.769 117 79 2 7 122 14 129 6.81e-15 70.9
Msa0838290 AT3G09910.5 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0838290 AT3G09910.4 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0838290 AT4G09720.4 28.409 176 106 5 6 170 8 174 1.45e-14 69.7
Msa0838290 AT3G63150.1 30.286 175 112 5 7 180 15 180 2.34e-14 71.2
Msa0838290 AT5G20020.1 28.485 165 103 4 7 170 14 164 8.48e-14 67.8
Msa0838290 AT5G20010.1 28.485 165 103 4 7 170 14 164 9.29e-14 67.8
Msa0838290 AT5G55190.1 31.092 119 80 2 7 124 14 131 9.68e-14 67.4
Msa0838290 AT4G09720.3 27.473 182 106 5 6 170 8 180 1.17e-13 67.4
Msa0838290 AT5G03520.2 30.137 146 90 5 47 191 47 181 1.91e-12 63.9
Msa0838290 AT4G35860.2 25.373 134 87 4 47 179 2 123 1.00e-11 61.2

Find 80 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAGATCTGAGGCTATTT+TGG 0.133154 5_4:+12649544 Msa0838290:five_prime_UTR
CTTTGTTCCAACCAGAATTA+TGG 0.197915 5_4:-12651014 None:intergenic
AGAAAGAGAAAGAACCTATT+TGG 0.261379 5_4:-12649261 None:intergenic
AGTGCGAATGTGGTTGTTAA+TGG 0.319066 5_4:+12650553 Msa0838290:CDS
TGAAATCAATAATAATAATA+AGG 0.321196 5_4:+12649091 None:intergenic
TAATGGAAGTATTGTGAATC+TGG 0.328007 5_4:+12650570 Msa0838290:CDS
TCTTCTGCTTTGGTGGTTGA+AGG 0.339738 5_4:-12651932 None:intergenic
AAACGACATTTCTTGTCATT+TGG 0.355939 5_4:+12652274 Msa0838290:three_prime_UTR
TTTGTTCCAACCAGAATTAT+GGG 0.374664 5_4:-12651013 None:intergenic
TGGTTGAAGGACAACTCTTA+TGG 0.386728 5_4:-12651919 None:intergenic
TTATGAAAATGTCTCCAAAA+AGG 0.394383 5_4:+12650787 Msa0838290:CDS
AAAGAAGGGGAGGGGAATAC+AGG 0.394999 5_4:-12652033 None:intergenic
CACACTCTCAGCTTCCAAAT+AGG 0.400756 5_4:+12649247 Msa0838290:five_prime_UTR
TTCTTTGTTATTGAAGAAGT+TGG 0.406930 5_4:+12652064 Msa0838290:three_prime_UTR
TTGTATCGAGATAGACCTTC+GGG 0.409474 5_4:+12651572 Msa0838290:intron
TTAAGCCACCTTATGCTTCA+AGG 0.411941 5_4:-12652129 None:intergenic
ATCACCTGAGCTGTGGTAAT+GGG 0.421707 5_4:-12651633 None:intergenic
GAAGAATAAGAAGAAAGTAC+TGG 0.425254 5_4:+12649501 Msa0838290:five_prime_UTR
AACCGTGGGGAAAGTATTGC+TGG 0.429322 5_4:-12649663 None:intergenic
CAAAGAAAATGATGAAAAGA+AGG 0.438811 5_4:-12652048 None:intergenic
TAAGAAGAAAGTACTGGAGG+AGG 0.446434 5_4:+12649507 Msa0838290:five_prime_UTR
AAAGAAGAATAAAGCACAAA+AGG 0.449553 5_4:+12651956 Msa0838290:CDS
AATCACCTGAGCTGTGGTAA+TGG 0.454383 5_4:-12651634 None:intergenic
TAATTGCCTTCTTCTCTCAC+AGG 0.456105 5_4:+12649292 Msa0838290:five_prime_UTR
ACCTGGTGTCCCCATAATTC+TGG 0.467734 5_4:+12651003 Msa0838290:CDS
CTAGATCTCCTTGAAGCATA+AGG 0.474002 5_4:+12652121 Msa0838290:three_prime_UTR
AAGAGGATATACGAAATAGT+TGG 0.475651 5_4:+12649182 Msa0838290:five_prime_UTR
AAAGAAAATGATGAAAAGAA+GGG 0.477205 5_4:-12652047 None:intergenic
GGTGCATAATGCTTCAACTC+TGG 0.481713 5_4:-12650983 None:intergenic
GGAGGAGGGGAACTGTCCTC+AGG 0.484285 5_4:+12649522 Msa0838290:five_prime_UTR
AAGAAGAAAGTACTGGAGGA+GGG 0.485036 5_4:+12649508 Msa0838290:five_prime_UTR
AGTGAATTACAATATTGAAC+AGG 0.490190 5_4:-12651979 None:intergenic
CCTAACCCTGTGAGAGAAGA+AGG 0.494457 5_4:-12649298 None:intergenic
ATTCTGGTTGGAACAAAGCT+TGG 0.495346 5_4:+12651019 Msa0838290:CDS
TTTGTATCGAGATAGACCTT+CGG 0.498207 5_4:+12651571 Msa0838290:intron
GTGGTAATGGGAACGGCACC+AGG 0.503169 5_4:-12651621 None:intergenic
TATTGTGAATCTGGGTTTGT+GGG 0.507703 5_4:+12650579 Msa0838290:CDS
AATGGAAGTATTGTGAATCT+GGG 0.518446 5_4:+12650571 Msa0838290:CDS
AATGATGAAAAGAAGGGGAG+GGG 0.524862 5_4:-12652041 None:intergenic
CTGGGTTTGTGGGATACTGC+TGG 0.538632 5_4:+12650589 Msa0838290:CDS
GTATTGTGAATCTGGGTTTG+TGG 0.538694 5_4:+12650578 Msa0838290:CDS
CGTTCCCATTACCACAGCTC+AGG 0.544810 5_4:+12651629 Msa0838290:CDS
TGAATGCAGTTCAAAATCAC+AGG 0.553565 5_4:+12651780 Msa0838290:CDS
AAATGTGTTACTGTTGGTGA+TGG 0.556572 5_4:+12649605 Msa0838290:CDS
TTTATTAAATGTGTTACTGT+TGG 0.563795 5_4:+12649599 Msa0838290:CDS
CACCAGCAATACTTTCCCCA+CGG 0.567542 5_4:+12649661 Msa0838290:CDS
GAACTGCATTCAATGTAAGC+TGG 0.572826 5_4:-12651769 None:intergenic
AAATGATGAAAAGAAGGGGA+GGG 0.575158 5_4:-12652042 None:intergenic
CAGTTCTGCATAGACCATCC+TGG 0.577967 5_4:+12651603 Msa0838290:CDS
AAGAAAATGATGAAAAGAAG+GGG 0.582810 5_4:-12652046 None:intergenic
GTTCAATATTGTAATTCACT+AGG 0.584735 5_4:+12651982 Msa0838290:exon
GAATAAGAAGAAAGTACTGG+AGG 0.584899 5_4:+12649504 Msa0838290:five_prime_UTR
AATTGCCTTCTTCTCTCACA+GGG 0.587915 5_4:+12649293 Msa0838290:five_prime_UTR
AAAATGATGAAAAGAAGGGG+AGG 0.591003 5_4:-12652043 None:intergenic
TATTTGTGGAACAGGGCAAG+AGG 0.600984 5_4:+12650685 Msa0838290:intron
ACCAGAATTATGGGGACACC+AGG 0.601533 5_4:-12651004 None:intergenic
ACTGTTGGTGATGGAGCTGT+TGG 0.601926 5_4:+12649614 Msa0838290:CDS
GTATTTATTGCAGAACGTGA+AGG 0.606998 5_4:+12651881 Msa0838290:intron
AGAAGAAAGTACTGGAGGAG+GGG 0.608480 5_4:+12649509 Msa0838290:five_prime_UTR
GGTGTCCCCATAATTCTGGT+TGG 0.615899 5_4:+12651007 Msa0838290:CDS
GAGTTGAAGCATTATGCACC+TGG 0.616427 5_4:+12650986 Msa0838290:CDS
TCGGCACCACGGTAACTCAA+AGG 0.619029 5_4:-12650725 None:intergenic
TATTAAAGGGAGAAGAGCTG+AGG 0.626553 5_4:+12651731 Msa0838290:intron
TTGTTCCAACCAGAATTATG+GGG 0.627746 5_4:-12651012 None:intergenic
CTGAGCTGTGGTAATGGGAA+CGG 0.629176 5_4:-12651628 None:intergenic
TGTCCTCAGGTGAAGATCTG+AGG 0.631328 5_4:+12649535 Msa0838290:five_prime_UTR
GAAAGCCAATATGAAAACAT+CGG 0.632883 5_4:-12650744 None:intergenic
TTAAGACCTTTGAGTTACCG+TGG 0.650534 5_4:+12650719 Msa0838290:CDS
AGAAGTTGGAGAGTCTGTGT+GGG 0.653335 5_4:+12652078 Msa0838290:three_prime_UTR
TGTGTTAACAACGAAACGAA+CGG 0.653824 5_4:+12649210 Msa0838290:five_prime_UTR
TGACAATTTCAGTGCGAATG+TGG 0.655180 5_4:+12650543 Msa0838290:CDS
AGAACTGCTTATCATCCCGA+AGG 0.656406 5_4:-12651587 None:intergenic
AAGAAGTTGGAGAGTCTGTG+TGG 0.657940 5_4:+12652077 Msa0838290:three_prime_UTR
TAATGGGAACGGCACCAGGA+TGG 0.658560 5_4:-12651617 None:intergenic
TTTATTGCAGAACGTGAAGG+CGG 0.659717 5_4:+12651884 Msa0838290:intron
GATCTCCTTGAAGCATAAGG+TGG 0.664504 5_4:+12652124 Msa0838290:three_prime_UTR
GGGGAATACAGGTAGAACAA+AGG 0.683373 5_4:-12652022 None:intergenic
ATGCAGTTCAAAATCACAGG+AGG 0.690232 5_4:+12651783 Msa0838290:CDS
TAGCCTCAGATCTTCACCTG+AGG 0.705958 5_4:-12649538 None:intergenic
ATATGAAAACATCGGCACCA+CGG 0.709782 5_4:-12650736 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTCAAATTTTTATTTT+TGG + chr5_4:12649723-12649742 Msa0838290:intron 10.0%
!!! AATTGAAAATTTTGATTTTT+CGG + chr5_4:12649787-12649806 Msa0838290:intron 10.0%
!! TAATGTCTTTAAATTAAAAA+AGG + chr5_4:12650091-12650110 Msa0838290:intron 10.0%
!!! TTGTTATTTGATTTATATTT+TGG + chr5_4:12650619-12650638 Msa0838290:intron 10.0%
!!! GTTTTTTTTTTTATGTATTT+TGG + chr5_4:12650851-12650870 Msa0838290:intron 10.0%
!! TTAATACCATATATAAATAT+GGG - chr5_4:12651093-12651112 None:intergenic 10.0%
!! ATTAATACCATATATAAATA+TGG - chr5_4:12651094-12651113 None:intergenic 10.0%
!! AAATTGAAGAAAAAAGAAAA+AGG - chr5_4:12649701-12649720 None:intergenic 15.0%
!!! TTTGGAATTTATATGTAATT+GGG + chr5_4:12650201-12650220 Msa0838290:intron 15.0%
!!! AGTTTGTTGAATTTAAAATT+TGG + chr5_4:12650227-12650246 Msa0838290:intron 15.0%
!!! GTTTGTTGAATTTAAAATTT+GGG + chr5_4:12650228-12650247 Msa0838290:intron 15.0%
!!! TTATTTGATTTATATTTTGG+TGG + chr5_4:12650622-12650641 Msa0838290:intron 15.0%
!! TAATACCATATATAAATATG+GGG - chr5_4:12651092-12651111 None:intergenic 15.0%
!! ATTTATATATGGTATTAATC+AGG + chr5_4:12651095-12651114 Msa0838290:intron 15.0%
!! TTTATATATGGTATTAATCA+GGG + chr5_4:12651096-12651115 Msa0838290:intron 15.0%
!! GAAAATATTTAATACAAATC+TGG - chr5_4:12651434-12651453 None:intergenic 15.0%
!!! TTTATTAAATGTGTTACTGT+TGG + chr5_4:12649599-12649618 Msa0838290:CDS 20.0%
!!! AATTTTTATTTTTGGGTATC+TGG + chr5_4:12649731-12649750 Msa0838290:intron 20.0%
!!! ATTTTTATTTTTGGGTATCT+GGG + chr5_4:12649732-12649751 Msa0838290:intron 20.0%
!!! ATTCTTGTAAATTTGAAATC+TGG + chr5_4:12649828-12649847 Msa0838290:intron 20.0%
!! CAAATCATAATTTACATACT+CGG - chr5_4:12649878-12649897 None:intergenic 20.0%
!!! TAAATTATGATTTGGAATGA+TGG + chr5_4:12649884-12649903 Msa0838290:intron 20.0%
!!! AAATTATGATTTGGAATGAT+GGG + chr5_4:12649885-12649904 Msa0838290:intron 20.0%
!!! GTTTGGAATTTATATGTAAT+TGG + chr5_4:12650200-12650219 Msa0838290:intron 20.0%
!! AGTCATGTAAATTTCAAATT+TGG + chr5_4:12650269-12650288 Msa0838290:intron 20.0%
!! TGTAAATTTCAAATTTGGTA+TGG + chr5_4:12650274-12650293 Msa0838290:intron 20.0%
!! TAATTGTTGGAATTAAAAGT+TGG + chr5_4:12650412-12650431 Msa0838290:intron 20.0%
!! AATTGTTGGAATTAAAAGTT+GGG + chr5_4:12650413-12650432 Msa0838290:intron 20.0%
!!! ATATTGATTTGTGAATTTAC+TGG + chr5_4:12650456-12650475 Msa0838290:intron 20.0%
!! AAAAAAAAAAACACACACAA+GGG - chr5_4:12650843-12650862 None:intergenic 20.0%
!! TAAAAAAAAAAACACACACA+AGG - chr5_4:12650844-12650863 None:intergenic 20.0%
!! AATATTAAGTCGAAAAAAAC+TGG - chr5_4:12651531-12651550 None:intergenic 20.0%
!! GATACAAATGACAAAATAAT+AGG - chr5_4:12651559-12651578 None:intergenic 20.0%
!! ATTAAATTAAATCTGACATG+TGG + chr5_4:12651704-12651723 Msa0838290:intron 20.0%
!! TTAAATTAAATCTGACATGT+GGG + chr5_4:12651705-12651724 Msa0838290:intron 20.0%
!! AAAGAAAATGATGAAAAGAA+GGG - chr5_4:12652050-12652069 None:intergenic 20.0%
! ATCACAACTTTGTTCAAAAA+AGG + chr5_4:12649141-12649160 Msa0838290:five_prime_UTR 25.0%
! AATTGAGAAAATGCAGAATT+GGG - chr5_4:12649357-12649376 None:intergenic 25.0%
! TAATTGAGAAAATGCAGAAT+TGG - chr5_4:12649358-12649377 None:intergenic 25.0%
!! AATTCTGCATTTTCTCAATT+AGG + chr5_4:12649357-12649376 Msa0838290:intron 25.0%
!! ATTCTGCATTTTCTCAATTA+GGG + chr5_4:12649358-12649377 Msa0838290:intron 25.0%
! CGAGTATGTAAATTATGATT+TGG + chr5_4:12649876-12649895 Msa0838290:intron 25.0%
! ACATTTAGTGTTTGATGTAA+AGG + chr5_4:12650346-12650365 Msa0838290:intron 25.0%
!! ATGGATATAATGTGAGATTT+TGG + chr5_4:12650374-12650393 Msa0838290:intron 25.0%
! TTGAGTTTATGTGTAATTGT+TGG + chr5_4:12650399-12650418 Msa0838290:intron 25.0%
!!! TTTTGGTGGATTTTGTTATT+CGG + chr5_4:12650636-12650655 Msa0838290:intron 25.0%
! GGATTTATTGTGTTTATTTG+TGG + chr5_4:12650671-12650690 Msa0838290:intron 25.0%
! TTATGAAAATGTCTCCAAAA+AGG + chr5_4:12650787-12650806 Msa0838290:CDS 25.0%
!!! TATGTATTTTGGTTTATCTG+CGG + chr5_4:12650862-12650881 Msa0838290:intron 25.0%
! ATATATAAATATGGGGAAGA+TGG - chr5_4:12651085-12651104 None:intergenic 25.0%
! ATCTTCCCCATATTTATATA+TGG + chr5_4:12651084-12651103 Msa0838290:intron 25.0%
! TAGTTGATTATGAAATGCAT+GGG - chr5_4:12651148-12651167 None:intergenic 25.0%
! ATAGTTGATTATGAAATGCA+TGG - chr5_4:12651149-12651168 None:intergenic 25.0%
! AAAACTAAAACAAACCAGAA+AGG - chr5_4:12651372-12651391 None:intergenic 25.0%
!!! ATTTTTTATTCTGCTACTCT+AGG + chr5_4:12651654-12651673 Msa0838290:intron 25.0%
!! TTGTTTCTTCTAATTCACAA+TGG + chr5_4:12651826-12651845 Msa0838290:intron 25.0%
!! AATGTGAAGTTTAAGTTTGT+TGG + chr5_4:12651856-12651875 Msa0838290:intron 25.0%
! AAAGAAGAATAAAGCACAAA+AGG + chr5_4:12651956-12651975 Msa0838290:CDS 25.0%
! AGTGAATTACAATATTGAAC+AGG - chr5_4:12651982-12652001 None:intergenic 25.0%
! GTTCAATATTGTAATTCACT+AGG + chr5_4:12651982-12652001 Msa0838290:exon 25.0%
! AAGAAAATGATGAAAAGAAG+GGG - chr5_4:12652049-12652068 None:intergenic 25.0%
! CAAAGAAAATGATGAAAAGA+AGG - chr5_4:12652051-12652070 None:intergenic 25.0%
!!! TTCTTTGTTATTGAAGAAGT+TGG + chr5_4:12652064-12652083 Msa0838290:three_prime_UTR 25.0%
AAGAGGATATACGAAATAGT+TGG + chr5_4:12649182-12649201 Msa0838290:five_prime_UTR 30.0%
AGAAAGAGAAAGAACCTATT+TGG - chr5_4:12649264-12649283 None:intergenic 30.0%
AATGCAGAATTGGGAAATTT+GGG - chr5_4:12649348-12649367 None:intergenic 30.0%
AAATGCAGAATTGGGAAATT+TGG - chr5_4:12649349-12649368 None:intergenic 30.0%
! GAAGAATAAGAAGAAAGTAC+TGG + chr5_4:12649501-12649520 Msa0838290:five_prime_UTR 30.0%
AATTCAAAGTTGAAAAAGCC+AGG - chr5_4:12649771-12649790 None:intergenic 30.0%
!!! AATTTTGATTTTTCGGCGTT+TGG + chr5_4:12649794-12649813 Msa0838290:intron 30.0%
! TGTAAATTTGAAATCTGGCA+TGG + chr5_4:12649833-12649852 Msa0838290:intron 30.0%
!! ATGGGTTGGATGTTAATTTA+AGG + chr5_4:12649903-12649922 Msa0838290:intron 30.0%
! TGGGTTGGATGTTAATTTAA+GGG + chr5_4:12649904-12649923 Msa0838290:intron 30.0%
! TGTTAATTTAAGGGTGTGTT+TGG + chr5_4:12649913-12649932 Msa0838290:intron 30.0%
! GTTAATTTAAGGGTGTGTTT+GGG + chr5_4:12649914-12649933 Msa0838290:intron 30.0%
TGTATCCATTTCAACCTTTA+GGG - chr5_4:12650167-12650186 None:intergenic 30.0%
TTGTATCCATTTCAACCTTT+AGG - chr5_4:12650168-12650187 None:intergenic 30.0%
GTTTGATGTAAAGGTTGAAA+TGG + chr5_4:12650355-12650374 Msa0838290:intron 30.0%
!! ATTTTGTCGAACTTGCTTTT+TGG + chr5_4:12650490-12650509 Msa0838290:intron 30.0%
CTGAAATTGTCAAAAACTGT+CGG - chr5_4:12650535-12650554 None:intergenic 30.0%
!! TAATGGAAGTATTGTGAATC+TGG + chr5_4:12650570-12650589 Msa0838290:CDS 30.0%
!! AATGGAAGTATTGTGAATCT+GGG + chr5_4:12650571-12650590 Msa0838290:CDS 30.0%
!!! TTTGGTGGATTTTGTTATTC+GGG + chr5_4:12650637-12650656 Msa0838290:intron 30.0%
ATTGTGTTTATTTGTGGAAC+AGG + chr5_4:12650677-12650696 Msa0838290:intron 30.0%
TTGTGTTTATTTGTGGAACA+GGG + chr5_4:12650678-12650697 Msa0838290:intron 30.0%
GAAAGCCAATATGAAAACAT+CGG - chr5_4:12650747-12650766 None:intergenic 30.0%
! TTTGGAGACATTTTCATAAC+TGG - chr5_4:12650786-12650805 None:intergenic 30.0%
! TTCTTGTTCCTATTTTGCTT+TGG + chr5_4:12650814-12650833 Msa0838290:intron 30.0%
TTTGTTCCAACCAGAATTAT+GGG - chr5_4:12651016-12651035 None:intergenic 30.0%
AGTTGATTATGAAATGCATG+GGG - chr5_4:12651147-12651166 None:intergenic 30.0%
AGATACCAGTAGAAAAACAA+TGG - chr5_4:12651313-12651332 None:intergenic 30.0%
CATACATTGTATCTGCAATT+GGG + chr5_4:12651396-12651415 Msa0838290:intron 30.0%
ATACATTGTATCTGCAATTG+GGG + chr5_4:12651397-12651416 Msa0838290:intron 30.0%
TTAATACAAATCTGGAGACA+GGG - chr5_4:12651426-12651445 None:intergenic 30.0%
TTTAATACAAATCTGGAGAC+AGG - chr5_4:12651427-12651446 None:intergenic 30.0%
! TTCTTCTTTTTCTTCTGCTT+TGG - chr5_4:12651945-12651964 None:intergenic 30.0%
TGTGTTAACAACGAAACGAA+CGG + chr5_4:12649210-12649229 Msa0838290:five_prime_UTR 35.0%
GGAAATTTGGGGAAAAAAGA+AGG - chr5_4:12649336-12649355 None:intergenic 35.0%
ATGCAGAATTGGGAAATTTG+GGG - chr5_4:12649347-12649366 None:intergenic 35.0%
!! GAATAAGAAGAAAGTACTGG+AGG + chr5_4:12649504-12649523 Msa0838290:five_prime_UTR 35.0%
!! TGAAGATCTGAGGCTATTTT+GGG + chr5_4:12649545-12649564 Msa0838290:five_prime_UTR 35.0%
!! TTTTCAAGATGAGTGCTTCT+AGG + chr5_4:12649576-12649595 Msa0838290:exon 35.0%
! AAATGTGTTACTGTTGGTGA+TGG + chr5_4:12649605-12649624 Msa0838290:CDS 35.0%
!! TATGATTTGGAATGATGGGT+TGG + chr5_4:12649889-12649908 Msa0838290:intron 35.0%
AGGTTGAAATGGATACAATG+TGG + chr5_4:12650170-12650189 Msa0838290:intron 35.0%
GGTTGAAATGGATACAATGT+GGG + chr5_4:12650171-12650190 Msa0838290:intron 35.0%
! ATGGATACAATGTGGGATTT+TGG + chr5_4:12650178-12650197 Msa0838290:intron 35.0%
!!! TACAATGTGGGATTTTGGTT+TGG + chr5_4:12650183-12650202 Msa0838290:intron 35.0%
!! GGTATGGAAAGTACTTTGTA+AGG + chr5_4:12650290-12650309 Msa0838290:intron 35.0%
! AAAGTACTTTGTAAGGCAAC+TGG + chr5_4:12650297-12650316 Msa0838290:intron 35.0%
AAGTACTTTGTAAGGCAACT+GGG + chr5_4:12650298-12650317 Msa0838290:intron 35.0%
TATTGTGAATCTGGGTTTGT+GGG + chr5_4:12650579-12650598 Msa0838290:CDS 35.0%
TCTGCGGTGAACAATATATA+CGG + chr5_4:12650878-12650897 Msa0838290:intron 35.0%
TTGTTCCAACCAGAATTATG+GGG - chr5_4:12651015-12651034 None:intergenic 35.0%
CTTTGTTCCAACCAGAATTA+TGG - chr5_4:12651017-12651036 None:intergenic 35.0%
!! ACCAGTTCTTTTTGTTTGCT+AGG + chr5_4:12651225-12651244 Msa0838290:intron 35.0%
!! CCAGTTCTTTTTGTTTGCTA+GGG + chr5_4:12651226-12651245 Msa0838290:intron 35.0%
!! CAGTTCTTTTTGTTTGCTAG+GGG + chr5_4:12651227-12651246 Msa0838290:intron 35.0%
!! AGTTCTTTTTGTTTGCTAGG+GGG + chr5_4:12651228-12651247 Msa0838290:intron 35.0%
!! CATCACCATTGTTTTTCTAC+TGG + chr5_4:12651305-12651324 Msa0838290:intron 35.0%
! GGTATCTGATACATCAGTTT+TGG + chr5_4:12651326-12651345 Msa0838290:intron 35.0%
!! TTTTGATGTTTCTGCCTTTC+TGG + chr5_4:12651355-12651374 Msa0838290:intron 35.0%
CCAATTGCAGATACAATGTA+TGG - chr5_4:12651398-12651417 None:intergenic 35.0%
CCATACATTGTATCTGCAAT+TGG + chr5_4:12651395-12651414 Msa0838290:intron 35.0%
AGCTAACAAAACTACTCGAT+GGG - chr5_4:12651466-12651485 None:intergenic 35.0%
AAGCTAACAAAACTACTCGA+TGG - chr5_4:12651467-12651486 None:intergenic 35.0%
TTAGCTTCCAGTTAGACAAA+TGG + chr5_4:12651480-12651499 Msa0838290:intron 35.0%
AGTACAACCATTTGTCTAAC+TGG - chr5_4:12651490-12651509 None:intergenic 35.0%
AAAAAACTGGTACACTGATC+AGG - chr5_4:12651518-12651537 None:intergenic 35.0%
TTTGTATCGAGATAGACCTT+CGG + chr5_4:12651571-12651590 Msa0838290:intron 35.0%
ATAAAAAATCACCTGAGCTG+TGG - chr5_4:12651643-12651662 None:intergenic 35.0%
TGAATGCAGTTCAAAATCAC+AGG + chr5_4:12651780-12651799 Msa0838290:CDS 35.0%
! TCAAAATCACAGGAGGTATA+TGG + chr5_4:12651790-12651809 Msa0838290:intron 35.0%
GTATTTATTGCAGAACGTGA+AGG + chr5_4:12651881-12651900 Msa0838290:intron 35.0%
! TTCTTTTTCTTCTGCTTTGG+TGG - chr5_4:12651942-12651961 None:intergenic 35.0%
AAATGATGAAAAGAAGGGGA+GGG - chr5_4:12652045-12652064 None:intergenic 35.0%
AAAATGATGAAAAGAAGGGG+AGG - chr5_4:12652046-12652065 None:intergenic 35.0%
TAATTGCCTTCTTCTCTCAC+AGG + chr5_4:12649292-12649311 Msa0838290:five_prime_UTR 40.0%
AATTGCCTTCTTCTCTCACA+GGG + chr5_4:12649293-12649312 Msa0838290:five_prime_UTR 40.0%
GGGGAAAAAAGAAGGAAATG+AGG - chr5_4:12649328-12649347 None:intergenic 40.0%
!! TAAGAAGAAAGTACTGGAGG+AGG + chr5_4:12649507-12649526 Msa0838290:five_prime_UTR 40.0%
!! AAGAAGAAAGTACTGGAGGA+GGG + chr5_4:12649508-12649527 Msa0838290:five_prime_UTR 40.0%
!! GTGAAGATCTGAGGCTATTT+TGG + chr5_4:12649544-12649563 Msa0838290:five_prime_UTR 40.0%
CTGGGTTGTGTTTAAATTCC+TGG + chr5_4:12649750-12649769 Msa0838290:intron 40.0%
GGCATGGAAAATGCTTTGTA+AGG + chr5_4:12649849-12649868 Msa0838290:intron 40.0%
!! TCAACCTTTAGGGTGTGTTT+GGG - chr5_4:12650157-12650176 None:intergenic 40.0%
!! TTCAACCTTTAGGGTGTGTT+TGG - chr5_4:12650158-12650177 None:intergenic 40.0%
ACACACCCTAAAGGTTGAAA+TGG + chr5_4:12650159-12650178 Msa0838290:intron 40.0%
!!! TGTCGAACTTGCTTTTTGGT+TGG + chr5_4:12650494-12650513 Msa0838290:intron 40.0%
!!! CTTGCTTTTTGGTTGGAAAC+AGG + chr5_4:12650501-12650520 Msa0838290:intron 40.0%
TGACAATTTCAGTGCGAATG+TGG + chr5_4:12650543-12650562 Msa0838290:CDS 40.0%
AGTGCGAATGTGGTTGTTAA+TGG + chr5_4:12650553-12650572 Msa0838290:CDS 40.0%
GTATTGTGAATCTGGGTTTG+TGG + chr5_4:12650578-12650597 Msa0838290:CDS 40.0%
! TTAAGACCTTTGAGTTACCG+TGG + chr5_4:12650719-12650738 Msa0838290:CDS 40.0%
ATATGAAAACATCGGCACCA+CGG - chr5_4:12650739-12650758 None:intergenic 40.0%
!!! TGGTGCCGATGTTTTCATAT+TGG + chr5_4:12650739-12650758 Msa0838290:CDS 40.0%
!!! GGAACAAGAAAGCACCTTTT+TGG - chr5_4:12650804-12650823 None:intergenic 40.0%
AAGGGACACCAAAGCAAAAT+AGG - chr5_4:12650825-12650844 None:intergenic 40.0%
CTGCGGTGAACAATATATAC+GGG + chr5_4:12650879-12650898 Msa0838290:intron 40.0%
! CGTGCTTGTTTCATTTTCAG+TGG + chr5_4:12650957-12650976 Msa0838290:intron 40.0%
! ATTCTGGTTGGAACAAAGCT+TGG + chr5_4:12651019-12651038 Msa0838290:CDS 40.0%
GAAGTTTCCATTTGTCGAAC+TGG + chr5_4:12651191-12651210 Msa0838290:intron 40.0%
CCCTAGCAAACAAAAAGAAC+TGG - chr5_4:12651229-12651248 None:intergenic 40.0%
!! TTTTTGTTTGCTAGGGGGAA+GGG + chr5_4:12651233-12651252 Msa0838290:intron 40.0%
TTGTATCGAGATAGACCTTC+GGG + chr5_4:12651572-12651591 Msa0838290:intron 40.0%
TGACATGTGGGTCCTATTAA+AGG + chr5_4:12651717-12651736 Msa0838290:intron 40.0%
GACATGTGGGTCCTATTAAA+GGG + chr5_4:12651718-12651737 Msa0838290:intron 40.0%
TCAGCTCTTCTCCCTTTAAT+AGG - chr5_4:12651732-12651751 None:intergenic 40.0%
TATTAAAGGGAGAAGAGCTG+AGG + chr5_4:12651731-12651750 Msa0838290:intron 40.0%
GAACTGCATTCAATGTAAGC+TGG - chr5_4:12651772-12651791 None:intergenic 40.0%
ATGCAGTTCAAAATCACAGG+AGG + chr5_4:12651783-12651802 Msa0838290:CDS 40.0%
TTTATTGCAGAACGTGAAGG+CGG + chr5_4:12651884-12651903 Msa0838290:intron 40.0%
TGGTTGAAGGACAACTCTTA+TGG - chr5_4:12651922-12651941 None:intergenic 40.0%
AATGATGAAAAGAAGGGGAG+GGG - chr5_4:12652044-12652063 None:intergenic 40.0%
CTAGATCTCCTTGAAGCATA+AGG + chr5_4:12652121-12652140 Msa0838290:three_prime_UTR 40.0%
TTAAGCCACCTTATGCTTCA+AGG - chr5_4:12652132-12652151 None:intergenic 40.0%
CACACTCTCAGCTTCCAAAT+AGG + chr5_4:12649247-12649266 Msa0838290:five_prime_UTR 45.0%
CTTCTTCTCTCACAGGGTTA+GGG + chr5_4:12649299-12649318 Msa0838290:intron 45.0%
!! AGAAGAAAGTACTGGAGGAG+GGG + chr5_4:12649509-12649528 Msa0838290:five_prime_UTR 45.0%
GAGAAGAATGACAAACCGTG+GGG - chr5_4:12649679-12649698 None:intergenic 45.0%
GGAGAAGAATGACAAACCGT+GGG - chr5_4:12649680-12649699 None:intergenic 45.0%
AGGAGAAGAATGACAAACCG+TGG - chr5_4:12649681-12649700 None:intergenic 45.0%
! TTAAGGGTGTGTTTGGGAAG+AGG + chr5_4:12649920-12649939 Msa0838290:intron 45.0%
! TAAGGGTGTGTTTGGGAAGA+GGG + chr5_4:12649921-12649940 Msa0838290:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+AGG + chr5_4:12649927-12649946 Msa0838290:intron 45.0%
TTTGGGAAGAGGGTTTAGGA+GGG + chr5_4:12649931-12649950 Msa0838290:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+GGG - chr5_4:12649927-12649946 None:intergenic 45.0%
TTAGGGTGTGTTTGGGAAGA+GGG - chr5_4:12650150-12650169 None:intergenic 45.0%
! TTTAGGGTGTGTTTGGGAAG+AGG - chr5_4:12650151-12650170 None:intergenic 45.0%
TCTTCCCAAACACACCCTAA+AGG + chr5_4:12650150-12650169 Msa0838290:intron 45.0%
!!! GGTGGATTTTGTTATTCGGG+AGG + chr5_4:12650640-12650659 Msa0838290:intron 45.0%
GTTATTCGGGAGGTTCTTTC+TGG + chr5_4:12650650-12650669 Msa0838290:intron 45.0%
TATTTGTGGAACAGGGCAAG+AGG + chr5_4:12650685-12650704 Msa0838290:intron 45.0%
GGTGCATAATGCTTCAACTC+TGG - chr5_4:12650986-12651005 None:intergenic 45.0%
! GAGTTGAAGCATTATGCACC+TGG + chr5_4:12650986-12651005 Msa0838290:CDS 45.0%
CAGTTAGCCAGTTCGACAAA+TGG - chr5_4:12651201-12651220 None:intergenic 45.0%
!! CTTTTTGTTTGCTAGGGGGA+AGG + chr5_4:12651232-12651251 Msa0838290:intron 45.0%
!! TTTTGTTTGCTAGGGGGAAG+GGG + chr5_4:12651234-12651253 Msa0838290:intron 45.0%
AGAACTGCTTATCATCCCGA+AGG - chr5_4:12651590-12651609 None:intergenic 45.0%
ATCACCTGAGCTGTGGTAAT+GGG - chr5_4:12651636-12651655 None:intergenic 45.0%
AATCACCTGAGCTGTGGTAA+TGG - chr5_4:12651637-12651656 None:intergenic 45.0%
! TCTTCTGCTTTGGTGGTTGA+AGG - chr5_4:12651935-12651954 None:intergenic 45.0%
GGGGAATACAGGTAGAACAA+AGG - chr5_4:12652025-12652044 None:intergenic 45.0%
AAGAAGTTGGAGAGTCTGTG+TGG + chr5_4:12652077-12652096 Msa0838290:three_prime_UTR 45.0%
AGAAGTTGGAGAGTCTGTGT+GGG + chr5_4:12652078-12652097 Msa0838290:three_prime_UTR 45.0%
GATCTCCTTGAAGCATAAGG+TGG + chr5_4:12652124-12652143 Msa0838290:three_prime_UTR 45.0%
!! AATAAAATAATAAAAATAAG+AGG + chr5_4:12649165-12649184 Msa0838290:five_prime_UTR 5.0%
!!! AATTTCAAATTTTTATTTTT+GGG + chr5_4:12649724-12649743 Msa0838290:intron 5.0%
!! ATAAAAACTTAAAAAAATAT+GGG - chr5_4:12652218-12652237 None:intergenic 5.0%
!! AATAAAAACTTAAAAAAATA+TGG - chr5_4:12652219-12652238 None:intergenic 5.0%
CCTAACCCTGTGAGAGAAGA+AGG - chr5_4:12649301-12649320 None:intergenic 50.0%
CCTTCTTCTCTCACAGGGTT+AGG + chr5_4:12649298-12649317 Msa0838290:intron 50.0%
TGTCCTCAGGTGAAGATCTG+AGG + chr5_4:12649535-12649554 Msa0838290:five_prime_UTR 50.0%
TAGCCTCAGATCTTCACCTG+AGG - chr5_4:12649541-12649560 None:intergenic 50.0%
!! ACTGTTGGTGATGGAGCTGT+TGG + chr5_4:12649614-12649633 Msa0838290:CDS 50.0%
CACCAGCAATACTTTCCCCA+CGG + chr5_4:12649661-12649680 Msa0838290:CDS 50.0%
!! AACCGTGGGGAAAGTATTGC+TGG - chr5_4:12649666-12649685 None:intergenic 50.0%
GTTTGGGAAGAGGGTTTAGG+AGG + chr5_4:12649930-12649949 Msa0838290:intron 50.0%
TTGGGAAGAGGGTTTAGGAG+GGG + chr5_4:12649932-12649951 Msa0838290:intron 50.0%
AAGAGGGTTTAGGAGGGGAA+GGG + chr5_4:12649937-12649956 Msa0838290:intron 50.0%
AAGAGGGTTTGGGAAGGGAA+GGG - chr5_4:12650134-12650153 None:intergenic 50.0%
TTGGGAAGAGGGTTTGGGAA+GGG - chr5_4:12650139-12650158 None:intergenic 50.0%
TTTGGGAAGAGGGTTTGGGA+AGG - chr5_4:12650140-12650159 None:intergenic 50.0%
GTGTGTTTGGGAAGAGGGTT+TGG - chr5_4:12649926-12649945 None:intergenic 50.0%
ACCTGGTGTCCCCATAATTC+TGG + chr5_4:12651003-12651022 Msa0838290:CDS 50.0%
ACCAGAATTATGGGGACACC+AGG - chr5_4:12651007-12651026 None:intergenic 50.0%
GGTGTCCCCATAATTCTGGT+TGG + chr5_4:12651007-12651026 Msa0838290:CDS 50.0%
! TTTGTTTGCTAGGGGGAAGG+GGG + chr5_4:12651235-12651254 Msa0838290:intron 50.0%
CAGTTCTGCATAGACCATCC+TGG + chr5_4:12651603-12651622 Msa0838290:CDS 50.0%
!! CTGAGCTGTGGTAATGGGAA+CGG - chr5_4:12651631-12651650 None:intergenic 50.0%
AAAGAAGGGGAGGGGAATAC+AGG - chr5_4:12652036-12652055 None:intergenic 50.0%
GAAGAGGGTTTAGGAGGGGA+AGG + chr5_4:12649936-12649955 Msa0838290:intron 55.0%
GAAGAGGGTTTGGGAAGGGA+AGG - chr5_4:12650135-12650154 None:intergenic 55.0%
CTGGGTTTGTGGGATACTGC+TGG + chr5_4:12650589-12650608 Msa0838290:CDS 55.0%
TCGGCACCACGGTAACTCAA+AGG - chr5_4:12650728-12650747 None:intergenic 55.0%
! TTGTTTGCTAGGGGGAAGGG+GGG + chr5_4:12651236-12651255 Msa0838290:intron 55.0%
TAATGGGAACGGCACCAGGA+TGG - chr5_4:12651620-12651639 None:intergenic 55.0%
CGTTCCCATTACCACAGCTC+AGG + chr5_4:12651629-12651648 Msa0838290:CDS 55.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr5_4:12649940-12649959 Msa0838290:intron 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG + chr5_4:12649941-12649960 Msa0838290:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG + chr5_4:12649942-12649961 Msa0838290:intron 60.0%
GGTTTGGGAAGGGAAGGGAG+GGG - chr5_4:12650129-12650148 None:intergenic 60.0%
GGGTTTGGGAAGGGAAGGGA+GGG - chr5_4:12650130-12650149 None:intergenic 60.0%
AGGGTTTGGGAAGGGAAGGG+AGG - chr5_4:12650131-12650150 None:intergenic 60.0%
! TGTTTGCTAGGGGGAAGGGG+GGG + chr5_4:12651237-12651256 Msa0838290:intron 60.0%
!! GTGGTAATGGGAACGGCACC+AGG - chr5_4:12651624-12651643 None:intergenic 60.0%
GGAGGAGGGGAACTGTCCTC+AGG + chr5_4:12649522-12649541 Msa0838290:five_prime_UTR 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG + chr5_4:12649945-12649964 Msa0838290:intron 65.0%
TAGGAGGGGAAGGGAGGGGA+GGG + chr5_4:12649946-12649965 Msa0838290:intron 65.0%
TGGGAAGGGAAGGGAGGGGA+GGG - chr5_4:12650125-12650144 None:intergenic 65.0%
TTGGGAAGGGAAGGGAGGGG+AGG - chr5_4:12650126-12650145 None:intergenic 65.0%
AGGGGGAAGGGGGGGATTTC+TGG + chr5_4:12651245-12651264 Msa0838290:intron 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 12649106 12652295 12649106 ID=Msa0838290;Name=Msa0838290
chr5_4 mRNA 12649106 12652295 12649106 ID=Msa0838290-mRNA-1;Parent=Msa0838290;Name=Msa0838290-mRNA-1;_AED=0.13;_eAED=0.13;_QI=317|1|1|1|0.85|0.75|8|300|197
chr5_4 exon 12649106 12649314 12649106 ID=Msa0838290-mRNA-1:exon:5551;Parent=Msa0838290-mRNA-1
chr5_4 exon 12649476 12649682 12649476 ID=Msa0838290-mRNA-1:exon:5552;Parent=Msa0838290-mRNA-1
chr5_4 exon 12650523 12650610 12650523 ID=Msa0838290-mRNA-1:exon:5553;Parent=Msa0838290-mRNA-1
chr5_4 exon 12650699 12650808 12650699 ID=Msa0838290-mRNA-1:exon:5554;Parent=Msa0838290-mRNA-1
chr5_4 exon 12650977 12651040 12650977 ID=Msa0838290-mRNA-1:exon:5555;Parent=Msa0838290-mRNA-1
chr5_4 exon 12651586 12651650 12651586 ID=Msa0838290-mRNA-1:exon:5556;Parent=Msa0838290-mRNA-1
chr5_4 exon 12651739 12651804 12651739 ID=Msa0838290-mRNA-1:exon:5557;Parent=Msa0838290-mRNA-1
chr5_4 exon 12651894 12652295 12651894 ID=Msa0838290-mRNA-1:exon:5558;Parent=Msa0838290-mRNA-1
chr5_4 five_prime_UTR 12649106 12649314 12649106 ID=Msa0838290-mRNA-1:five_prime_utr;Parent=Msa0838290-mRNA-1
chr5_4 five_prime_UTR 12649476 12649583 12649476 ID=Msa0838290-mRNA-1:five_prime_utr;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12649584 12649682 12649584 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12650523 12650610 12650523 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12650699 12650808 12650699 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12650977 12651040 12650977 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12651586 12651650 12651586 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12651739 12651804 12651739 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 CDS 12651894 12651995 12651894 ID=Msa0838290-mRNA-1:cds;Parent=Msa0838290-mRNA-1
chr5_4 three_prime_UTR 12651996 12652295 12651996 ID=Msa0838290-mRNA-1:three_prime_utr;Parent=Msa0838290-mRNA-1
Gene Sequence

>Msa0838290

ATGAGTGCTTCTAGGTTTATTAAATGTGTTACTGTTGGTGATGGAGCTGTTGGAAAAACTTGTTTGTTGATTTCTTACACCAGCAATACTTTCCCCACGGATTATGTGCCGACAGTTTTTGACAATTTCAGTGCGAATGTGGTTGTTAATGGAAGTATTGTGAATCTGGGTTTGTGGGATACTGCTGGGCAAGAGGATTATAACAGATTAAGACCTTTGAGTTACCGTGGTGCCGATGTTTTCATATTGGCTTTCTCTCTCATAAGCAAAGCCAGTTATGAAAATGTCTCCAAAAAGTGGATTCCAGAGTTGAAGCATTATGCACCTGGTGTCCCCATAATTCTGGTTGGAACAAAGCTTGACCTTCGGGATGATAAGCAGTTCTGCATAGACCATCCTGGTGCCGTTCCCATTACCACAGCTCAGGGAGAAGAGCTGAGGAAGCTGATTAATGCACCAGCTTACATTGAATGCAGTTCAAAATCACAGGAGAACGTGAAGGCGGTGTTTGACGCAGCCATAAGAGTTGTCCTTCAACCACCAAAGCAGAAGAAAAAGAAGAATAAAGCACAAAAGGCCTGTTCAATATTGTAA
Protein sequence

>Msa0838290

MSASRFIKCVTVGDGAVGKTCLLISYTSNTFPTDYVPTVFDNFSANVVVNGSIVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIILVGTKLDLRDDKQFCIDHPGAVPITTAQGEELRKLINAPAYIECSSKSQENVKAVFDAAIRVVLQPPKQKKKKNKAQKACSIL*