AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0800300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800300 MtrunA17_Chr5g0405791 100.000 197 0 0 1 197 1 197 2.40e-147 407
Msa0800300 MtrunA17_Chr8g0371501 97.462 197 5 0 1 197 1 197 1.10e-143 397
Msa0800300 MtrunA17_Chr4g0037021 89.340 197 21 0 1 197 1 197 1.93e-133 372
Msa0800300 MtrunA17_Chr3g0117931 77.835 194 42 1 2 194 4 197 1.41e-116 330
Msa0800300 MtrunA17_Chr2g0325101 84.270 178 28 0 1 178 1 178 8.01e-116 327
Msa0800300 MtrunA17_Chr6g0486041 74.874 199 47 1 1 196 1 199 1.40e-111 317
Msa0800300 MtrunA17_Chr4g0046211 88.112 143 17 0 1 143 1 143 5.57e-94 270
Msa0800300 MtrunA17_Chr7g0265001 30.851 188 116 5 8 192 18 194 1.09e-25 98.6
Msa0800300 MtrunA17_Chr1g0182321 31.383 188 116 6 6 191 15 191 7.56e-25 96.7
Msa0800300 MtrunA17_Chr1g0182301 31.383 188 116 6 6 191 15 191 1.31e-24 95.9
Msa0800300 MtrunA17_Chr1g0212521 32.386 176 106 6 6 179 15 179 1.60e-24 95.9
Msa0800300 MtrunA17_Chr4g0030911 32.386 176 106 6 6 179 15 179 2.78e-24 95.1
Msa0800300 MtrunA17_Chr7g0263071 30.851 188 117 6 6 191 15 191 3.86e-24 94.7
Msa0800300 MtrunA17_Chr8g0384311 30.726 179 109 5 6 181 13 179 2.64e-23 92.4
Msa0800300 MtrunA17_Chr3g0130271 29.630 189 119 5 7 192 13 190 3.95e-23 92.0
Msa0800300 MtrunA17_Chr1g0199331 31.461 178 105 7 7 180 32 196 8.76e-23 90.9
Msa0800300 MtrunA17_Chr1g0180291 30.899 178 108 5 7 181 15 180 1.44e-22 90.5
Msa0800300 MtrunA17_Chr8g0349711 30.814 172 105 5 1 170 1 160 2.85e-22 89.7
Msa0800300 MtrunA17_Chr8g0335471 33.516 182 102 8 6 180 8 177 4.21e-22 89.0
Msa0800300 MtrunA17_Chr1g0159481 30.726 179 109 5 6 181 17 183 4.99e-22 89.4
Msa0800300 MtrunA17_Chr7g0275991 33.939 165 95 5 8 170 22 174 5.37e-22 89.4
Msa0800300 MtrunA17_Chr4g0055651 29.947 187 112 6 6 185 8 182 6.03e-22 88.6
Msa0800300 MtrunA17_Chr8g0390561 31.111 180 109 6 8 184 11 178 1.42e-21 87.4
Msa0800300 MtrunA17_Chr7g0250531 31.016 187 110 7 6 185 8 182 1.53e-21 87.4
Msa0800300 MtrunA17_Chr4g0055081 32.727 165 97 5 8 170 11 163 2.05e-21 87.4
Msa0800300 MtrunA17_Chr2g0276931 30.857 175 104 6 1 170 1 163 2.79e-21 87.0
Msa0800300 MtrunA17_Chr3g0109721 32.961 179 105 5 8 183 12 178 8.98e-21 85.5
Msa0800300 MtrunA17_Chr2g0319951 29.282 181 114 5 6 184 13 181 1.01e-20 85.9
Msa0800300 MtrunA17_Chr5g0400131 32.727 165 97 5 8 170 11 163 1.97e-20 84.7
Msa0800300 MtrunA17_Chr2g0327911 29.891 184 114 6 6 185 15 187 2.55e-20 84.7
Msa0800300 MtrunA17_Chr8g0346421 38.655 119 70 3 8 124 28 145 2.68e-20 85.1
Msa0800300 MtrunA17_Chr6g0458081 27.624 181 117 5 1 179 1 169 2.72e-20 84.3
Msa0800300 MtrunA17_Chr2g0290891 27.907 172 110 5 1 170 1 160 3.85e-20 84.0
Msa0800300 MtrunA17_Chr8g0349681 30.233 172 106 5 1 170 8 167 8.83e-20 86.3
Msa0800300 MtrunA17_Chr8g0349681 29.651 172 107 5 1 170 379 538 5.21e-19 84.3
Msa0800300 MtrunA17_Chr8g0349681 28.488 172 109 5 1 170 170 329 1.22e-18 83.2
Msa0800300 MtrunA17_Chr5g0433561 31.515 165 99 4 8 170 12 164 2.27e-19 81.6
Msa0800300 MtrunA17_Chr4g0040781 35.606 132 82 3 6 135 13 143 3.82e-19 81.6
Msa0800300 MtrunA17_Chr1g0201121 30.729 192 116 7 1 184 1 183 4.18e-19 81.3
Msa0800300 MtrunA17_Chr3g0123971 30.909 165 100 4 8 170 11 163 4.46e-19 81.3
Msa0800300 MtrunA17_Chr4g0046261 29.834 181 113 5 6 184 13 181 4.61e-19 81.3
Msa0800300 MtrunA17_Chr2g0276941 30.994 171 104 5 6 174 15 173 4.87e-19 81.3
Msa0800300 MtrunA17_Chr3g0112681 29.834 181 113 5 6 184 12 180 5.46e-19 81.3
Msa0800300 MtrunA17_Chr2g0315551 35.833 120 74 3 6 123 13 131 5.74e-19 81.3
Msa0800300 MtrunA17_Chr1g0174281 32.530 166 98 5 7 170 16 169 6.21e-19 81.3
Msa0800300 MtrunA17_Chr8g0382221 31.515 165 99 5 8 170 11 163 6.56e-19 80.9
Msa0800300 MtrunA17_Chr2g0283131 35.833 120 74 3 6 123 13 131 1.05e-18 80.5
Msa0800300 MtrunA17_Chr1g0197331 28.804 184 116 5 7 186 16 188 1.31e-18 80.1
Msa0800300 MtrunA17_Chr7g0234201 28.947 190 121 5 6 193 13 190 1.69e-18 79.7
Msa0800300 MtrunA17_Chr7g0231131 31.928 166 99 6 7 170 16 169 2.65e-18 79.3
Msa0800300 MtrunA17_Chr8g0381841 31.515 165 99 5 8 170 19 171 6.75e-18 78.6
Msa0800300 MtrunA17_Chr8g0392931 35.294 119 74 3 6 122 14 131 1.19e-17 77.8
Msa0800300 MtrunA17_Chr2g0331691 29.032 186 117 6 6 185 10 186 1.38e-17 77.4
Msa0800300 MtrunA17_Chr7g0214241 28.947 190 121 5 6 193 15 192 1.53e-17 77.4
Msa0800300 MtrunA17_Chr4g0034871 27.957 186 119 5 6 185 8 184 2.64e-17 76.6
Msa0800300 MtrunA17_Chr3g0123821 31.325 166 98 6 8 170 18 170 3.04e-17 76.6
Msa0800300 MtrunA17_Chr6g0466301 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0800300 MtrunA17_Chr6g0465491 30.994 171 103 6 6 170 8 169 4.79e-17 75.9
Msa0800300 MtrunA17_Chr4g0032201 29.341 167 104 5 6 170 12 166 5.92e-17 75.9
Msa0800300 MtrunA17_Chr1g0189361 30.114 176 105 6 6 177 14 175 6.15e-17 75.9
Msa0800300 MtrunA17_Chr8g0351861 31.138 167 101 6 6 170 16 170 6.27e-17 75.9
Msa0800300 MtrunA17_Chr2g0325091 36.667 120 73 3 6 123 11 129 1.74e-16 74.3
Msa0800300 MtrunA17_Chr4g0004661 28.497 193 121 7 1 185 57 240 2.52e-16 74.7
Msa0800300 MtrunA17_Chr8g0383261 28.235 170 109 3 6 170 12 173 2.58e-16 73.9
Msa0800300 MtrunA17_Chr6g0482721 33.065 124 80 3 6 127 12 134 9.61e-16 72.4
Msa0800300 MtrunA17_Chr7g0259771 28.877 187 99 4 7 180 9 174 4.67e-15 72.8
Msa0800300 MtrunA17_Chr3g0138121 28.485 165 103 4 7 170 14 164 6.94e-14 67.4
Msa0800300 MtrunA17_Chr3g0138101 28.485 165 103 4 7 170 14 164 7.16e-14 67.4
Msa0800300 MtrunA17_Chr3g0138111 28.485 165 103 4 7 170 14 164 8.00e-14 67.4
Msa0800300 MtrunA17_Chr1g0185591 27.604 192 95 6 7 180 13 178 8.38e-14 69.3
Msa0800300 MtrunA17_Chr1g0191811 28.571 175 115 4 7 180 17 182 8.40e-13 66.2
Msa0800300 MtrunA17_Chr4g0046221 70.270 37 11 0 142 178 10 46 2.23e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0800300 AT2G17800.2 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0800300 AT2G17800.1 95.431 197 9 0 1 197 1 197 8.11e-128 358
Msa0800300 AT3G51300.1 95.431 197 9 0 1 197 1 197 1.40e-127 357
Msa0800300 AT4G35950.1 95.939 197 8 0 1 197 1 197 1.42e-127 357
Msa0800300 AT4G35020.1 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0800300 AT4G35020.2 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0800300 AT4G35020.3 87.374 198 24 1 1 197 1 198 2.34e-124 349
Msa0800300 AT1G75840.1 91.573 178 15 0 1 178 1 178 5.36e-122 343
Msa0800300 AT1G20090.1 90.341 176 17 0 3 178 2 177 3.09e-119 336
Msa0800300 AT5G45970.1 86.034 179 25 0 1 179 1 179 1.71e-117 332
Msa0800300 AT4G28950.1 75.500 200 43 2 1 196 1 198 2.63e-111 317
Msa0800300 AT5G62880.1 80.447 179 35 0 2 180 4 182 8.69e-111 315
Msa0800300 AT3G48040.1 80.447 179 35 0 2 180 4 182 8.96e-110 313
Msa0800300 AT2G44690.1 75.000 192 48 0 6 197 18 209 2.40e-106 304
Msa0800300 AT5G62880.2 79.070 172 32 1 2 173 4 171 5.27e-103 295
Msa0800300 AT3G48040.2 80.368 163 32 0 2 164 4 166 4.32e-100 287
Msa0800300 AT3G51290.2 95.122 164 8 0 34 197 635 798 1.77e-96 297
Msa0800300 AT4G35020.4 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0800300 AT4G35020.5 92.500 120 9 0 1 120 1 120 9.82e-82 239
Msa0800300 AT3G46060.2 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0800300 AT3G46060.3 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0800300 AT3G46060.1 32.386 176 106 6 6 179 15 179 7.98e-24 94.4
Msa0800300 AT3G54840.2 33.333 168 97 6 7 171 34 189 8.90e-24 93.6
Msa0800300 AT3G54840.1 32.143 168 99 5 7 171 34 189 9.53e-24 93.6
Msa0800300 AT3G54840.3 31.928 166 98 5 9 171 2 155 3.62e-23 91.3
Msa0800300 AT1G02130.1 32.544 169 100 6 6 172 8 164 8.47e-23 91.3
Msa0800300 AT3G53610.2 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0800300 AT3G53610.3 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0800300 AT3G53610.1 31.737 167 101 6 6 170 15 170 1.04e-22 91.3
Msa0800300 AT5G45130.1 33.333 165 96 4 8 170 12 164 1.10e-22 90.9
Msa0800300 AT5G59840.1 31.737 167 101 5 6 170 15 170 1.43e-22 90.9
Msa0800300 AT4G19640.1 33.333 165 96 4 8 170 12 164 2.49e-22 90.1
Msa0800300 AT1G43890.3 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0800300 AT1G43890.1 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0800300 AT1G43890.2 30.168 179 110 5 6 181 13 179 2.50e-22 90.1
Msa0800300 AT1G16920.1 32.418 182 109 6 6 185 13 182 2.99e-22 90.1
Msa0800300 AT5G45130.2 33.129 163 95 4 10 170 22 172 5.58e-22 89.4
Msa0800300 AT1G73640.1 32.984 191 110 6 6 190 13 191 1.97e-21 88.6
Msa0800300 AT2G44610.1 33.333 165 96 5 8 170 11 163 2.27e-21 87.8
Msa0800300 AT5G03530.2 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0800300 AT5G03530.1 28.191 188 120 5 8 192 15 190 2.39e-21 87.8
Msa0800300 AT4G18430.1 32.597 181 108 5 6 184 13 181 4.12e-21 87.0
Msa0800300 AT4G35860.1 27.624 181 117 5 1 179 1 169 1.11e-20 85.9
Msa0800300 AT4G17530.1 31.016 187 110 7 6 185 8 182 1.79e-20 85.1
Msa0800300 AT4G39890.1 34.731 167 92 6 8 170 11 164 1.82e-20 85.5
Msa0800300 AT5G47200.1 31.319 182 106 7 6 180 8 177 3.03e-20 84.7
Msa0800300 AT3G11730.1 29.940 167 103 5 6 170 8 162 3.37e-20 84.3
Msa0800300 AT2G22290.1 33.333 165 96 5 8 170 11 163 3.58e-20 84.3
Msa0800300 AT1G06400.1 32.335 167 99 5 6 170 13 167 6.72e-20 84.0
Msa0800300 AT4G19640.2 36.885 122 74 3 8 127 12 132 8.89e-20 82.0
Msa0800300 AT4G18800.1 36.667 120 73 3 6 123 13 131 9.53e-20 83.6
Msa0800300 AT1G09630.1 31.492 181 110 5 6 184 12 180 9.58e-20 83.6
Msa0800300 AT3G15060.1 31.285 179 109 5 8 184 15 181 1.65e-19 82.8
Msa0800300 AT4G17170.1 27.326 172 111 5 1 170 1 160 2.17e-19 82.4
Msa0800300 AT4G19640.3 34.074 135 86 3 8 140 12 145 2.68e-19 82.0
Msa0800300 AT2G31680.1 29.670 182 114 4 6 185 12 181 2.75e-19 82.4
Msa0800300 AT3G09900.1 32.934 167 99 6 6 170 15 170 3.29e-19 82.0
Msa0800300 AT3G07410.1 30.526 190 118 5 6 193 12 189 3.29e-19 82.0
Msa0800300 AT4G17160.1 26.744 172 112 5 1 170 1 160 3.69e-19 81.6
Msa0800300 AT1G18200.1 30.387 181 112 6 6 184 13 181 5.73e-19 81.6
Msa0800300 AT5G60860.1 30.387 181 112 5 6 184 13 181 5.85e-19 81.6
Msa0800300 AT2G43130.1 29.121 182 115 5 6 185 12 181 5.89e-19 81.3
Msa0800300 AT3G12160.1 32.530 166 98 5 7 170 16 169 5.91e-19 81.6
Msa0800300 AT5G45750.1 35.833 120 74 3 6 123 13 131 6.31e-19 81.3
Msa0800300 AT3G18820.1 29.570 186 116 6 6 185 8 184 7.28e-19 80.9
Msa0800300 AT5G03520.1 33.533 167 98 6 6 170 15 170 8.63e-19 80.9
Msa0800300 AT1G01200.1 34.132 167 95 6 7 170 29 183 1.66e-18 80.9
Msa0800300 AT1G28550.1 37.500 120 72 3 6 123 13 131 1.71e-18 80.1
Msa0800300 AT5G10260.1 32.121 165 98 5 8 170 11 163 2.11e-18 79.7
Msa0800300 AT1G05810.2 30.994 171 104 5 6 174 58 216 2.97e-18 80.5
Msa0800300 AT1G05810.1 29.670 182 114 5 6 185 12 181 3.26e-18 79.7
Msa0800300 AT2G33870.1 37.815 119 71 3 6 122 13 130 5.00e-18 79.0
Msa0800300 AT5G64990.1 33.333 165 96 5 8 170 9 161 7.57e-18 78.2
Msa0800300 AT5G47520.1 28.652 178 113 5 2 177 10 175 1.85e-17 77.4
Msa0800300 AT1G22740.1 29.167 192 119 6 1 184 1 183 2.47e-17 77.0
Msa0800300 AT1G52280.1 30.726 179 107 7 1 171 1 170 5.25e-17 76.3
Msa0800300 AT2G21880.1 27.174 184 121 4 6 184 9 184 9.54e-17 75.5
Msa0800300 AT4G39990.1 32.787 122 79 3 7 126 18 138 1.10e-16 75.5
Msa0800300 AT1G07410.1 34.454 119 75 3 6 122 12 129 1.27e-16 75.1
Msa0800300 AT5G59150.1 29.240 171 107 6 6 174 12 170 1.57e-16 75.1
Msa0800300 AT3G16100.1 29.947 187 114 7 1 179 1 178 1.87e-16 74.7
Msa0800300 AT5G47960.1 33.051 118 76 3 7 122 16 132 2.16e-16 74.7
Msa0800300 AT5G65270.1 28.729 181 113 6 7 184 18 185 2.31e-16 74.7
Msa0800300 AT4G09720.1 30.409 171 104 6 6 170 8 169 5.43e-16 73.2
Msa0800300 AT3G09910.3 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0800300 AT3G09910.1 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0800300 AT3G09910.2 28.652 178 112 5 7 181 14 179 1.09e-15 72.4
Msa0800300 AT5G27540.1 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0800300 AT5G27540.2 25.862 174 121 2 7 180 18 183 1.12e-15 75.1
Msa0800300 AT3G46830.1 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0800300 AT3G46830.2 32.773 119 77 3 6 122 12 129 1.39e-15 72.4
Msa0800300 AT1G49300.2 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0800300 AT1G49300.1 29.825 171 105 6 6 170 8 169 1.51e-15 72.0
Msa0800300 AT5G64990.2 31.977 172 96 6 8 170 9 168 3.92e-15 71.2
Msa0800300 AT5G55080.1 30.769 117 79 2 7 122 14 129 6.81e-15 70.9
Msa0800300 AT3G09910.5 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0800300 AT3G09910.4 29.282 181 110 6 7 181 14 182 9.10e-15 69.7
Msa0800300 AT4G09720.4 28.409 176 106 5 6 170 8 174 1.45e-14 69.7
Msa0800300 AT3G63150.1 30.286 175 112 5 7 180 15 180 2.34e-14 71.2
Msa0800300 AT5G20020.1 28.485 165 103 4 7 170 14 164 8.48e-14 67.8
Msa0800300 AT5G20010.1 28.485 165 103 4 7 170 14 164 9.29e-14 67.8
Msa0800300 AT5G55190.1 31.092 119 80 2 7 124 14 131 9.68e-14 67.4
Msa0800300 AT4G09720.3 27.473 182 106 5 6 170 8 180 1.17e-13 67.4
Msa0800300 AT5G03520.2 30.137 146 90 5 47 191 47 181 1.91e-12 63.9
Msa0800300 AT4G35860.2 25.373 134 87 4 47 179 2 123 1.00e-11 61.2

Find 80 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAGATCTGAGGCTATTT+TGG 0.133154 5_3:+13659952 Msa0800300:five_prime_UTR
CTTTGTTCCAACCAGAATTA+TGG 0.197915 5_3:-13661415 None:intergenic
AGAAAGAGAAAGAACCTATT+TGG 0.261379 5_3:-13659668 None:intergenic
AGTGCGAATGTGGTTGTTAA+TGG 0.319066 5_3:+13660961 Msa0800300:CDS
TGAAATCAATAATAATAATA+AGG 0.321196 5_3:+13659500 None:intergenic
TAATGGAAGTATTGTGAATC+TGG 0.328007 5_3:+13660978 Msa0800300:CDS
TCTTCTGCTTTGGTGGTTGA+AGG 0.339738 5_3:-13662329 None:intergenic
AAACGACATTTCTTGTCATT+TGG 0.355939 5_3:+13662669 Msa0800300:three_prime_UTR
TTTGTTCCAACCAGAATTAT+GGG 0.374664 5_3:-13661414 None:intergenic
TGGTTGAAGGACAACTCTTA+TGG 0.386728 5_3:-13662316 None:intergenic
TTATGAAAATGTCTCCAAAA+AGG 0.394383 5_3:+13661196 Msa0800300:CDS
AAAGAAGGGGAGGGGAATAC+AGG 0.394999 5_3:-13662430 None:intergenic
CACACTCTCAGCTTCCAAAT+AGG 0.400756 5_3:+13659654 Msa0800300:five_prime_UTR
TTCTTTGTTATTGAAGAAGT+TGG 0.406930 5_3:+13662461 Msa0800300:three_prime_UTR
TTGTATCGAGATAGACCTTC+GGG 0.409474 5_3:+13661969 Msa0800300:intron
TTAAGCCACCTTATGCTTCA+AGG 0.411941 5_3:-13662526 None:intergenic
ATCACCTGAGCTGTGGTAAT+GGG 0.421707 5_3:-13662030 None:intergenic
GAAGAATAAGAAGAAAGTAC+TGG 0.425254 5_3:+13659909 Msa0800300:five_prime_UTR
AACCGTGGGGAAAGTATTGC+TGG 0.429322 5_3:-13660071 None:intergenic
CAAAGAAAATGATGAAAAGA+AGG 0.438811 5_3:-13662445 None:intergenic
TAAGAAGAAAGTACTGGAGG+AGG 0.446434 5_3:+13659915 Msa0800300:five_prime_UTR
AAAGAAGAATAAAGCACAAA+AGG 0.449553 5_3:+13662353 Msa0800300:CDS
AATCACCTGAGCTGTGGTAA+TGG 0.454383 5_3:-13662031 None:intergenic
TAATTGCCTTCTTCTCTCAC+AGG 0.456105 5_3:+13659699 Msa0800300:five_prime_UTR
ACCTGGTGTCCCCATAATTC+TGG 0.467734 5_3:+13661404 Msa0800300:CDS
CTAGATCTCCTTGAAGCATA+AGG 0.474002 5_3:+13662518 Msa0800300:three_prime_UTR
AAGAGGATATACGAAATAGT+TGG 0.475651 5_3:+13659589 Msa0800300:five_prime_UTR
AAAGAAAATGATGAAAAGAA+GGG 0.477205 5_3:-13662444 None:intergenic
GGTGCATAATGCTTCAACTC+TGG 0.481713 5_3:-13661384 None:intergenic
GGAGGAGGGGAACTGTCCTC+AGG 0.484285 5_3:+13659930 Msa0800300:five_prime_UTR
AAGAAGAAAGTACTGGAGGA+GGG 0.485036 5_3:+13659916 Msa0800300:five_prime_UTR
AGTGAATTACAATATTGAAC+AGG 0.490190 5_3:-13662376 None:intergenic
CCTAACCCTGTGAGAGAAGA+AGG 0.494457 5_3:-13659705 None:intergenic
ATTCTGGTTGGAACAAAGCT+TGG 0.495346 5_3:+13661420 Msa0800300:CDS
TTTGTATCGAGATAGACCTT+CGG 0.498207 5_3:+13661968 Msa0800300:intron
GTGGTAATGGGAACGGCACC+AGG 0.503169 5_3:-13662018 None:intergenic
TATTGTGAATCTGGGTTTGT+GGG 0.507703 5_3:+13660987 Msa0800300:CDS
AATGGAAGTATTGTGAATCT+GGG 0.518446 5_3:+13660979 Msa0800300:CDS
AATGATGAAAAGAAGGGGAG+GGG 0.524862 5_3:-13662438 None:intergenic
CTGGGTTTGTGGGATACTGC+TGG 0.538632 5_3:+13660997 Msa0800300:CDS
GTATTGTGAATCTGGGTTTG+TGG 0.538694 5_3:+13660986 Msa0800300:CDS
CGTTCCCATTACCACAGCTC+AGG 0.544810 5_3:+13662026 Msa0800300:CDS
TGAATGCAGTTCAAAATCAC+AGG 0.553565 5_3:+13662177 Msa0800300:CDS
AAATGTGTTACTGTTGGTGA+TGG 0.556572 5_3:+13660013 Msa0800300:CDS
TTTATTAAATGTGTTACTGT+TGG 0.563795 5_3:+13660007 Msa0800300:CDS
CACCAGCAATACTTTCCCCA+CGG 0.567542 5_3:+13660069 Msa0800300:CDS
GAACTGCATTCAATGTAAGC+TGG 0.572826 5_3:-13662166 None:intergenic
AAATGATGAAAAGAAGGGGA+GGG 0.575158 5_3:-13662439 None:intergenic
CAGTTCTGCATAGACCATCC+TGG 0.577967 5_3:+13662000 Msa0800300:CDS
AAGAAAATGATGAAAAGAAG+GGG 0.582810 5_3:-13662443 None:intergenic
GTTCAATATTGTAATTCACT+AGG 0.584735 5_3:+13662379 Msa0800300:exon
GAATAAGAAGAAAGTACTGG+AGG 0.584899 5_3:+13659912 Msa0800300:five_prime_UTR
AATTGCCTTCTTCTCTCACA+GGG 0.587915 5_3:+13659700 Msa0800300:five_prime_UTR
AAAATGATGAAAAGAAGGGG+AGG 0.591003 5_3:-13662440 None:intergenic
TATTTGTGGAACAGGGCAAG+AGG 0.600984 5_3:+13661094 Msa0800300:intron
ACCAGAATTATGGGGACACC+AGG 0.601533 5_3:-13661405 None:intergenic
ACTGTTGGTGATGGAGCTGT+TGG 0.601926 5_3:+13660022 Msa0800300:CDS
GTATTTATTGCAGAACGTGA+AGG 0.606998 5_3:+13662278 Msa0800300:intron
AGAAGAAAGTACTGGAGGAG+GGG 0.608480 5_3:+13659917 Msa0800300:five_prime_UTR
GGTGTCCCCATAATTCTGGT+TGG 0.615899 5_3:+13661408 Msa0800300:CDS
GAGTTGAAGCATTATGCACC+TGG 0.616427 5_3:+13661387 Msa0800300:CDS
TCGGCACCACGGTAACTCAA+AGG 0.619029 5_3:-13661134 None:intergenic
TATTAAAGGGAGAAGAGCTG+AGG 0.626553 5_3:+13662128 Msa0800300:intron
TTGTTCCAACCAGAATTATG+GGG 0.627746 5_3:-13661413 None:intergenic
CTGAGCTGTGGTAATGGGAA+CGG 0.629176 5_3:-13662025 None:intergenic
TGTCCTCAGGTGAAGATCTG+AGG 0.631328 5_3:+13659943 Msa0800300:five_prime_UTR
GAAAGCCAATATGAAAACAT+CGG 0.632883 5_3:-13661153 None:intergenic
TTAAGACCTTTGAGTTACCG+TGG 0.650534 5_3:+13661128 Msa0800300:CDS
AGAAGTTGGAGAGTCTGTGT+GGG 0.653335 5_3:+13662475 Msa0800300:three_prime_UTR
TGTGTTAACAACGAAACGAA+CGG 0.653824 5_3:+13659617 Msa0800300:five_prime_UTR
TGACAATTTCAGTGCGAATG+TGG 0.655180 5_3:+13660951 Msa0800300:CDS
AGAACTGCTTATCATCCCGA+AGG 0.656406 5_3:-13661984 None:intergenic
AAGAAGTTGGAGAGTCTGTG+TGG 0.657940 5_3:+13662474 Msa0800300:three_prime_UTR
TAATGGGAACGGCACCAGGA+TGG 0.658560 5_3:-13662014 None:intergenic
TTTATTGCAGAACGTGAAGG+CGG 0.659717 5_3:+13662281 Msa0800300:intron
GATCTCCTTGAAGCATAAGG+TGG 0.664504 5_3:+13662521 Msa0800300:three_prime_UTR
GGGGAATACAGGTAGAACAA+AGG 0.683373 5_3:-13662419 None:intergenic
ATGCAGTTCAAAATCACAGG+AGG 0.690232 5_3:+13662180 Msa0800300:CDS
TAGCCTCAGATCTTCACCTG+AGG 0.705958 5_3:-13659946 None:intergenic
ATATGAAAACATCGGCACCA+CGG 0.709782 5_3:-13661145 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATTTCAAATTTTTATTTT+TGG + chr5_3:13660131-13660150 Msa0800300:intron 10.0%
!!! AATTGAAAATTTTGATTTTT+CGG + chr5_3:13660195-13660214 Msa0800300:intron 10.0%
!! TAATGTCTTTAAATTAAAAA+AGG + chr5_3:13660499-13660518 Msa0800300:intron 10.0%
!!! TTATTTTATTTATATTTTGG+TGG + chr5_3:13661030-13661049 Msa0800300:intron 10.0%
!! TTAATACCATATATAAATAT+GGG - chr5_3:13661494-13661513 None:intergenic 10.0%
!! ATTAATACCATATATAAATA+TGG - chr5_3:13661495-13661514 None:intergenic 10.0%
!! AAATTGAAGAAAAAAGAAAA+AGG - chr5_3:13660109-13660128 None:intergenic 15.0%
!!! TTTGGAATTTATATGTAATT+GGG + chr5_3:13660609-13660628 Msa0800300:intron 15.0%
!!! AGTTTGTTGAATTTAAAATT+TGG + chr5_3:13660635-13660654 Msa0800300:intron 15.0%
!!! GTTTGTTGAATTTAAAATTT+GGG + chr5_3:13660636-13660655 Msa0800300:intron 15.0%
!! AAATACATAAAAAAACACAA+GGG - chr5_3:13661252-13661271 None:intergenic 15.0%
!! AAAATACATAAAAAAACACA+AGG - chr5_3:13661253-13661272 None:intergenic 15.0%
!!! TTGTGTTTTTTTATGTATTT+TGG + chr5_3:13661252-13661271 Msa0800300:intron 15.0%
!! TAATACCATATATAAATATG+GGG - chr5_3:13661493-13661512 None:intergenic 15.0%
!! ATTTATATATGGTATTAATC+AGG + chr5_3:13661496-13661515 Msa0800300:intron 15.0%
!! TTTATATATGGTATTAATCA+GGG + chr5_3:13661497-13661516 Msa0800300:intron 15.0%
!! GAAAATATTTAATACAAATC+TGG - chr5_3:13661831-13661850 None:intergenic 15.0%
!!! TTTATTAAATGTGTTACTGT+TGG + chr5_3:13660007-13660026 Msa0800300:CDS 20.0%
!!! AATTTTTATTTTTGGGTATC+TGG + chr5_3:13660139-13660158 Msa0800300:intron 20.0%
!!! ATTTTTATTTTTGGGTATCT+GGG + chr5_3:13660140-13660159 Msa0800300:intron 20.0%
!!! ATTCTTGTAAATTTGAAATC+TGG + chr5_3:13660236-13660255 Msa0800300:intron 20.0%
!! CAAATCATAATTTACATACT+CGG - chr5_3:13660286-13660305 None:intergenic 20.0%
!!! TAAATTATGATTTGGAATGA+TGG + chr5_3:13660292-13660311 Msa0800300:intron 20.0%
!!! AAATTATGATTTGGAATGAT+GGG + chr5_3:13660293-13660312 Msa0800300:intron 20.0%
!!! GTTTGGAATTTATATGTAAT+TGG + chr5_3:13660608-13660627 Msa0800300:intron 20.0%
!! AGTCATGTAAATTTCAAATT+TGG + chr5_3:13660677-13660696 Msa0800300:intron 20.0%
!! TGTAAATTTCAAATTTGGTA+TGG + chr5_3:13660682-13660701 Msa0800300:intron 20.0%
!! TAATTGTTGGAATTAAAAGT+TGG + chr5_3:13660820-13660839 Msa0800300:intron 20.0%
!! AATTGTTGGAATTAAAAGTT+GGG + chr5_3:13660821-13660840 Msa0800300:intron 20.0%
!!! ATATTGATTTGTGAATTTAC+TGG + chr5_3:13660864-13660883 Msa0800300:intron 20.0%
!! GGATTTATTATGTTTATTTG+TGG + chr5_3:13661080-13661099 Msa0800300:intron 20.0%
!! AATATTAAGTCGAAAAAAAC+TGG - chr5_3:13661928-13661947 None:intergenic 20.0%
!! GATACAAATGACAAAATAAT+AGG - chr5_3:13661956-13661975 None:intergenic 20.0%
!! ATTAAATTAAATCTGACATG+TGG + chr5_3:13662101-13662120 Msa0800300:intron 20.0%
!! TTAAATTAAATCTGACATGT+GGG + chr5_3:13662102-13662121 Msa0800300:intron 20.0%
!! AAAGAAAATGATGAAAAGAA+GGG - chr5_3:13662447-13662466 None:intergenic 20.0%
! AATTGAGAAAATGCAGAATT+GGG - chr5_3:13659765-13659784 None:intergenic 25.0%
! TAATTGAGAAAATGCAGAAT+TGG - chr5_3:13659766-13659785 None:intergenic 25.0%
!! AATTCTGCATTTTCTCAATT+AGG + chr5_3:13659765-13659784 Msa0800300:intron 25.0%
!! ATTCTGCATTTTCTCAATTA+GGG + chr5_3:13659766-13659785 Msa0800300:intron 25.0%
! CGAGTATGTAAATTATGATT+TGG + chr5_3:13660284-13660303 Msa0800300:intron 25.0%
! ACATTTAGTGTTTGATGTAA+AGG + chr5_3:13660754-13660773 Msa0800300:intron 25.0%
!! ATGGATATAATGTGAGATTT+TGG + chr5_3:13660782-13660801 Msa0800300:intron 25.0%
! TTGAGTTTATGTGTAATTGT+TGG + chr5_3:13660807-13660826 Msa0800300:intron 25.0%
!!! TTTGGTGGATTTTTGTTATT+TGG + chr5_3:13661045-13661064 Msa0800300:intron 25.0%
!!! TTGGTGGATTTTTGTTATTT+GGG + chr5_3:13661046-13661065 Msa0800300:intron 25.0%
! ATTATGTTTATTTGTGGAAC+AGG + chr5_3:13661086-13661105 Msa0800300:intron 25.0%
! TTATGTTTATTTGTGGAACA+GGG + chr5_3:13661087-13661106 Msa0800300:intron 25.0%
! TTATGAAAATGTCTCCAAAA+AGG + chr5_3:13661196-13661215 Msa0800300:CDS 25.0%
!!! TATGTATTTTGGTTTATCTG+TGG + chr5_3:13661263-13661282 Msa0800300:intron 25.0%
! ATATATAAATATGGGGAAGA+TGG - chr5_3:13661486-13661505 None:intergenic 25.0%
! ATCTTCCCCATATTTATATA+TGG + chr5_3:13661485-13661504 Msa0800300:intron 25.0%
! TAGTTGATTATGAAATGCAT+GGG - chr5_3:13661549-13661568 None:intergenic 25.0%
! ATAGTTGATTATGAAATGCA+TGG - chr5_3:13661550-13661569 None:intergenic 25.0%
! AAAACTAAAACAAACCAGAA+AGG - chr5_3:13661770-13661789 None:intergenic 25.0%
!!! ATTTTTTATTCTGCTACTCT+AGG + chr5_3:13662051-13662070 Msa0800300:intron 25.0%
! TTGATTCTTCTAATTCACAA+TGG + chr5_3:13662223-13662242 Msa0800300:intron 25.0%
!! AATGTGAAGTTTAAGTTTGT+TGG + chr5_3:13662253-13662272 Msa0800300:intron 25.0%
! AAAGAAGAATAAAGCACAAA+AGG + chr5_3:13662353-13662372 Msa0800300:CDS 25.0%
! AGTGAATTACAATATTGAAC+AGG - chr5_3:13662379-13662398 None:intergenic 25.0%
! GTTCAATATTGTAATTCACT+AGG + chr5_3:13662379-13662398 Msa0800300:exon 25.0%
! AAGAAAATGATGAAAAGAAG+GGG - chr5_3:13662446-13662465 None:intergenic 25.0%
! CAAAGAAAATGATGAAAAGA+AGG - chr5_3:13662448-13662467 None:intergenic 25.0%
!!! TTCTTTGTTATTGAAGAAGT+TGG + chr5_3:13662461-13662480 Msa0800300:three_prime_UTR 25.0%
ATCACAACTTCGTTCAAAAA+AGG + chr5_3:13659548-13659567 Msa0800300:five_prime_UTR 30.0%
AAGAGGATATACGAAATAGT+TGG + chr5_3:13659589-13659608 Msa0800300:five_prime_UTR 30.0%
AGAAAGAGAAAGAACCTATT+TGG - chr5_3:13659671-13659690 None:intergenic 30.0%
GAAATTTGGGGAAAAAAAGA+AGG - chr5_3:13659743-13659762 None:intergenic 30.0%
AATGCAGAATTGGGAAATTT+GGG - chr5_3:13659756-13659775 None:intergenic 30.0%
AAATGCAGAATTGGGAAATT+TGG - chr5_3:13659757-13659776 None:intergenic 30.0%
! GAAGAATAAGAAGAAAGTAC+TGG + chr5_3:13659909-13659928 Msa0800300:five_prime_UTR 30.0%
AATTCAAAGTTGAAAAAGCC+AGG - chr5_3:13660179-13660198 None:intergenic 30.0%
!!! AATTTTGATTTTTCGGCGTT+TGG + chr5_3:13660202-13660221 Msa0800300:intron 30.0%
! TGTAAATTTGAAATCTGGCA+TGG + chr5_3:13660241-13660260 Msa0800300:intron 30.0%
!! ATGGGTTGGATGTTAATTTA+AGG + chr5_3:13660311-13660330 Msa0800300:intron 30.0%
! TGGGTTGGATGTTAATTTAA+GGG + chr5_3:13660312-13660331 Msa0800300:intron 30.0%
! TGTTAATTTAAGGGTGTGTT+TGG + chr5_3:13660321-13660340 Msa0800300:intron 30.0%
! GTTAATTTAAGGGTGTGTTT+GGG + chr5_3:13660322-13660341 Msa0800300:intron 30.0%
TGTATCCATTTCAACCTTTA+GGG - chr5_3:13660575-13660594 None:intergenic 30.0%
TTGTATCCATTTCAACCTTT+AGG - chr5_3:13660576-13660595 None:intergenic 30.0%
GTTTGATGTAAAGGTTGAAA+TGG + chr5_3:13660763-13660782 Msa0800300:intron 30.0%
!! ATTTTGTCGAACTTGCTTTT+TGG + chr5_3:13660898-13660917 Msa0800300:intron 30.0%
CTGAAATTGTCAAAAACTGT+CGG - chr5_3:13660943-13660962 None:intergenic 30.0%
!! TAATGGAAGTATTGTGAATC+TGG + chr5_3:13660978-13660997 Msa0800300:CDS 30.0%
!! AATGGAAGTATTGTGAATCT+GGG + chr5_3:13660979-13660998 Msa0800300:CDS 30.0%
GAAAGCCAATATGAAAACAT+CGG - chr5_3:13661156-13661175 None:intergenic 30.0%
! TTTGGAGACATTTTCATAAC+TGG - chr5_3:13661195-13661214 None:intergenic 30.0%
TCTGTGGTGAACAATATATA+CGG + chr5_3:13661279-13661298 Msa0800300:intron 30.0%
TTTGTTCCAACCAGAATTAT+GGG - chr5_3:13661417-13661436 None:intergenic 30.0%
AGTTGATTATGAAATGCATG+GGG - chr5_3:13661548-13661567 None:intergenic 30.0%
AGATACCAGTAGAAAAACAA+TGG - chr5_3:13661711-13661730 None:intergenic 30.0%
CATACATTGTATCTGCAATT+GGG + chr5_3:13661793-13661812 Msa0800300:intron 30.0%
ATACATTGTATCTGCAATTG+GGG + chr5_3:13661794-13661813 Msa0800300:intron 30.0%
TTAATACAAATCTGGAGACA+AGG - chr5_3:13661823-13661842 None:intergenic 30.0%
! TTCTTCTTTTTCTTCTGCTT+TGG - chr5_3:13662342-13662361 None:intergenic 30.0%
TGTGTTAACAACGAAACGAA+CGG + chr5_3:13659617-13659636 Msa0800300:five_prime_UTR 35.0%
GGGAAAAAAAGAAGGAAATG+AGG - chr5_3:13659735-13659754 None:intergenic 35.0%
ATGCAGAATTGGGAAATTTG+GGG - chr5_3:13659755-13659774 None:intergenic 35.0%
!! GAATAAGAAGAAAGTACTGG+AGG + chr5_3:13659912-13659931 Msa0800300:five_prime_UTR 35.0%
!! TGAAGATCTGAGGCTATTTT+GGG + chr5_3:13659953-13659972 Msa0800300:five_prime_UTR 35.0%
!! TTTTCAAGATGAGTGCTTCT+AGG + chr5_3:13659984-13660003 Msa0800300:exon 35.0%
! AAATGTGTTACTGTTGGTGA+TGG + chr5_3:13660013-13660032 Msa0800300:CDS 35.0%
!! TATGATTTGGAATGATGGGT+TGG + chr5_3:13660297-13660316 Msa0800300:intron 35.0%
AGGTTGAAATGGATACAATG+TGG + chr5_3:13660578-13660597 Msa0800300:intron 35.0%
GGTTGAAATGGATACAATGT+GGG + chr5_3:13660579-13660598 Msa0800300:intron 35.0%
! ATGGATACAATGTGGGATTT+TGG + chr5_3:13660586-13660605 Msa0800300:intron 35.0%
!!! TACAATGTGGGATTTTGGTT+TGG + chr5_3:13660591-13660610 Msa0800300:intron 35.0%
!! GGTATGGAAAGTACTTTGTA+AGG + chr5_3:13660698-13660717 Msa0800300:intron 35.0%
! AAAGTACTTTGTAAGGCAAC+TGG + chr5_3:13660705-13660724 Msa0800300:intron 35.0%
AAGTACTTTGTAAGGCAACT+GGG + chr5_3:13660706-13660725 Msa0800300:intron 35.0%
TATTGTGAATCTGGGTTTGT+GGG + chr5_3:13660987-13661006 Msa0800300:CDS 35.0%
!!! GTGGATTTTTGTTATTTGGG+AGG + chr5_3:13661049-13661068 Msa0800300:intron 35.0%
! TTCTTGTTCCCATTTTGCTT+TGG + chr5_3:13661223-13661242 Msa0800300:intron 35.0%
CTGTGGTGAACAATATATAC+GGG + chr5_3:13661280-13661299 Msa0800300:intron 35.0%
TTGTTCCAACCAGAATTATG+GGG - chr5_3:13661416-13661435 None:intergenic 35.0%
CTTTGTTCCAACCAGAATTA+TGG - chr5_3:13661418-13661437 None:intergenic 35.0%
!! CATCACCATTGTTTTTCTAC+TGG + chr5_3:13661703-13661722 Msa0800300:intron 35.0%
! GGTATCTGATACATCAGTTT+TGG + chr5_3:13661724-13661743 Msa0800300:intron 35.0%
!! TTTTGATGTTTCTGCCTTTC+TGG + chr5_3:13661753-13661772 Msa0800300:intron 35.0%
CCAATTGCAGATACAATGTA+TGG - chr5_3:13661795-13661814 None:intergenic 35.0%
CCATACATTGTATCTGCAAT+TGG + chr5_3:13661792-13661811 Msa0800300:intron 35.0%
AGCTAACAAAACTACTCGAT+GGG - chr5_3:13661863-13661882 None:intergenic 35.0%
AAGCTAACAAAACTACTCGA+TGG - chr5_3:13661864-13661883 None:intergenic 35.0%
TTAGCTTCCAGTTAGACAAA+TGG + chr5_3:13661877-13661896 Msa0800300:intron 35.0%
AAAAAACTGGTACACTGATC+AGG - chr5_3:13661915-13661934 None:intergenic 35.0%
TTTGTATCGAGATAGACCTT+CGG + chr5_3:13661968-13661987 Msa0800300:intron 35.0%
ATAAAAAATCACCTGAGCTG+TGG - chr5_3:13662040-13662059 None:intergenic 35.0%
TGAATGCAGTTCAAAATCAC+AGG + chr5_3:13662177-13662196 Msa0800300:CDS 35.0%
! TCAAAATCACAGGAGGTATA+TGG + chr5_3:13662187-13662206 Msa0800300:intron 35.0%
GTATTTATTGCAGAACGTGA+AGG + chr5_3:13662278-13662297 Msa0800300:intron 35.0%
! TTCTTTTTCTTCTGCTTTGG+TGG - chr5_3:13662339-13662358 None:intergenic 35.0%
AAATGATGAAAAGAAGGGGA+GGG - chr5_3:13662442-13662461 None:intergenic 35.0%
AAAATGATGAAAAGAAGGGG+AGG - chr5_3:13662443-13662462 None:intergenic 35.0%
TAATTGCCTTCTTCTCTCAC+AGG + chr5_3:13659699-13659718 Msa0800300:five_prime_UTR 40.0%
AATTGCCTTCTTCTCTCACA+GGG + chr5_3:13659700-13659719 Msa0800300:five_prime_UTR 40.0%
!! TAAGAAGAAAGTACTGGAGG+AGG + chr5_3:13659915-13659934 Msa0800300:five_prime_UTR 40.0%
!! AAGAAGAAAGTACTGGAGGA+GGG + chr5_3:13659916-13659935 Msa0800300:five_prime_UTR 40.0%
!! GTGAAGATCTGAGGCTATTT+TGG + chr5_3:13659952-13659971 Msa0800300:five_prime_UTR 40.0%
CTGGGTTGTGTTTAAATTCC+TGG + chr5_3:13660158-13660177 Msa0800300:intron 40.0%
GGCATGGAAAATGCTTTGTA+AGG + chr5_3:13660257-13660276 Msa0800300:intron 40.0%
!! TCAACCTTTAGGGTGTGTTT+GGG - chr5_3:13660565-13660584 None:intergenic 40.0%
!! TTCAACCTTTAGGGTGTGTT+TGG - chr5_3:13660566-13660585 None:intergenic 40.0%
ACACACCCTAAAGGTTGAAA+TGG + chr5_3:13660567-13660586 Msa0800300:intron 40.0%
!!! TGTCGAACTTGCTTTTTGGT+TGG + chr5_3:13660902-13660921 Msa0800300:intron 40.0%
!!! CTTGCTTTTTGGTTGGAAAC+AGG + chr5_3:13660909-13660928 Msa0800300:intron 40.0%
TGACAATTTCAGTGCGAATG+TGG + chr5_3:13660951-13660970 Msa0800300:CDS 40.0%
AGTGCGAATGTGGTTGTTAA+TGG + chr5_3:13660961-13660980 Msa0800300:CDS 40.0%
GTATTGTGAATCTGGGTTTG+TGG + chr5_3:13660986-13661005 Msa0800300:CDS 40.0%
GTTATTTGGGAGGTTCTTTC+TGG + chr5_3:13661059-13661078 Msa0800300:intron 40.0%
! TTAAGACCTTTGAGTTACCG+TGG + chr5_3:13661128-13661147 Msa0800300:CDS 40.0%
ATATGAAAACATCGGCACCA+CGG - chr5_3:13661148-13661167 None:intergenic 40.0%
!!! TGGTGCCGATGTTTTCATAT+TGG + chr5_3:13661148-13661167 Msa0800300:CDS 40.0%
!!! GGAACAAGAAAGCACCTTTT+TGG - chr5_3:13661213-13661232 None:intergenic 40.0%
AAGGGACACCAAAGCAAAAT+GGG - chr5_3:13661234-13661253 None:intergenic 40.0%
! CGTGCTTGTTTCATTTTCAG+TGG + chr5_3:13661358-13661377 Msa0800300:intron 40.0%
! ATTCTGGTTGGAACAAAGCT+TGG + chr5_3:13661420-13661439 Msa0800300:CDS 40.0%
GAAGTTTCCATTTGTCGAAC+TGG + chr5_3:13661592-13661611 Msa0800300:intron 40.0%
!! ACCGGTTCTTTTTGTTTGCT+AGG + chr5_3:13661626-13661645 Msa0800300:intron 40.0%
CCCTAGCAAACAAAAAGAAC+CGG - chr5_3:13661630-13661649 None:intergenic 40.0%
!! CCGGTTCTTTTTGTTTGCTA+GGG + chr5_3:13661627-13661646 Msa0800300:intron 40.0%
!! CGGTTCTTTTTGTTTGCTAG+GGG + chr5_3:13661628-13661647 Msa0800300:intron 40.0%
!! GGTTCTTTTTGTTTGCTAGG+GGG + chr5_3:13661629-13661648 Msa0800300:intron 40.0%
!! TTTTTGTTTGCTAGGGGGAA+GGG + chr5_3:13661634-13661653 Msa0800300:intron 40.0%
AGCACAACCATTTGTCTAAC+TGG - chr5_3:13661887-13661906 None:intergenic 40.0%
TTGTATCGAGATAGACCTTC+GGG + chr5_3:13661969-13661988 Msa0800300:intron 40.0%
TGACATGTGGGTCCTATTAA+AGG + chr5_3:13662114-13662133 Msa0800300:intron 40.0%
GACATGTGGGTCCTATTAAA+GGG + chr5_3:13662115-13662134 Msa0800300:intron 40.0%
TCAGCTCTTCTCCCTTTAAT+AGG - chr5_3:13662129-13662148 None:intergenic 40.0%
TATTAAAGGGAGAAGAGCTG+AGG + chr5_3:13662128-13662147 Msa0800300:intron 40.0%
GAACTGCATTCAATGTAAGC+TGG - chr5_3:13662169-13662188 None:intergenic 40.0%
ATGCAGTTCAAAATCACAGG+AGG + chr5_3:13662180-13662199 Msa0800300:CDS 40.0%
TTTATTGCAGAACGTGAAGG+CGG + chr5_3:13662281-13662300 Msa0800300:intron 40.0%
TGGTTGAAGGACAACTCTTA+TGG - chr5_3:13662319-13662338 None:intergenic 40.0%
AATGATGAAAAGAAGGGGAG+GGG - chr5_3:13662441-13662460 None:intergenic 40.0%
CTAGATCTCCTTGAAGCATA+AGG + chr5_3:13662518-13662537 Msa0800300:three_prime_UTR 40.0%
TTAAGCCACCTTATGCTTCA+AGG - chr5_3:13662529-13662548 None:intergenic 40.0%
CACACTCTCAGCTTCCAAAT+AGG + chr5_3:13659654-13659673 Msa0800300:five_prime_UTR 45.0%
CTTCTTCTCTCACAGGGTTA+GGG + chr5_3:13659706-13659725 Msa0800300:intron 45.0%
!! AGAAGAAAGTACTGGAGGAG+GGG + chr5_3:13659917-13659936 Msa0800300:five_prime_UTR 45.0%
GAGAAGAATGACAAACCGTG+GGG - chr5_3:13660087-13660106 None:intergenic 45.0%
GGAGAAGAATGACAAACCGT+GGG - chr5_3:13660088-13660107 None:intergenic 45.0%
AGGAGAAGAATGACAAACCG+TGG - chr5_3:13660089-13660108 None:intergenic 45.0%
! TTAAGGGTGTGTTTGGGAAG+AGG + chr5_3:13660328-13660347 Msa0800300:intron 45.0%
! TAAGGGTGTGTTTGGGAAGA+GGG + chr5_3:13660329-13660348 Msa0800300:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+AGG + chr5_3:13660335-13660354 Msa0800300:intron 45.0%
TTTGGGAAGAGGGTTTAGGA+GGG + chr5_3:13660339-13660358 Msa0800300:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+GGG - chr5_3:13660335-13660354 None:intergenic 45.0%
TTAGGGTGTGTTTGGGAAGA+GGG - chr5_3:13660558-13660577 None:intergenic 45.0%
! TTTAGGGTGTGTTTGGGAAG+AGG - chr5_3:13660559-13660578 None:intergenic 45.0%
TCTTCCCAAACACACCCTAA+AGG + chr5_3:13660558-13660577 Msa0800300:intron 45.0%
TTGTGGGATACTGCTGGTAA+TGG + chr5_3:13661003-13661022 Msa0800300:intron 45.0%
TATTTGTGGAACAGGGCAAG+AGG + chr5_3:13661094-13661113 Msa0800300:intron 45.0%
CAAGGGACACCAAAGCAAAA+TGG - chr5_3:13661235-13661254 None:intergenic 45.0%
GGTGCATAATGCTTCAACTC+TGG - chr5_3:13661387-13661406 None:intergenic 45.0%
! GAGTTGAAGCATTATGCACC+TGG + chr5_3:13661387-13661406 Msa0800300:CDS 45.0%
CAGTTAGCCAGTTCGACAAA+TGG - chr5_3:13661602-13661621 None:intergenic 45.0%
!! CTTTTTGTTTGCTAGGGGGA+AGG + chr5_3:13661633-13661652 Msa0800300:intron 45.0%
!! TTTTGTTTGCTAGGGGGAAG+GGG + chr5_3:13661635-13661654 Msa0800300:intron 45.0%
AGAACTGCTTATCATCCCGA+AGG - chr5_3:13661987-13662006 None:intergenic 45.0%
ATCACCTGAGCTGTGGTAAT+GGG - chr5_3:13662033-13662052 None:intergenic 45.0%
AATCACCTGAGCTGTGGTAA+TGG - chr5_3:13662034-13662053 None:intergenic 45.0%
! TCTTCTGCTTTGGTGGTTGA+AGG - chr5_3:13662332-13662351 None:intergenic 45.0%
GGGGAATACAGGTAGAACAA+AGG - chr5_3:13662422-13662441 None:intergenic 45.0%
AAGAAGTTGGAGAGTCTGTG+TGG + chr5_3:13662474-13662493 Msa0800300:three_prime_UTR 45.0%
AGAAGTTGGAGAGTCTGTGT+GGG + chr5_3:13662475-13662494 Msa0800300:three_prime_UTR 45.0%
GATCTCCTTGAAGCATAAGG+TGG + chr5_3:13662521-13662540 Msa0800300:three_prime_UTR 45.0%
!! AATAAAATAATAAAAATAAG+AGG + chr5_3:13659572-13659591 Msa0800300:five_prime_UTR 5.0%
!!! AATTTCAAATTTTTATTTTT+GGG + chr5_3:13660132-13660151 Msa0800300:intron 5.0%
!!! TTGTTATTTTATTTATATTT+TGG + chr5_3:13661027-13661046 Msa0800300:intron 5.0%
!! ATAAAAACTTAAAAAAATAT+GGG - chr5_3:13662613-13662632 None:intergenic 5.0%
!! AATAAAAACTTAAAAAAATA+TGG - chr5_3:13662614-13662633 None:intergenic 5.0%
CCTAACCCTGTGAGAGAAGA+AGG - chr5_3:13659708-13659727 None:intergenic 50.0%
CCTTCTTCTCTCACAGGGTT+AGG + chr5_3:13659705-13659724 Msa0800300:intron 50.0%
TGTCCTCAGGTGAAGATCTG+AGG + chr5_3:13659943-13659962 Msa0800300:five_prime_UTR 50.0%
TAGCCTCAGATCTTCACCTG+AGG - chr5_3:13659949-13659968 None:intergenic 50.0%
!! ACTGTTGGTGATGGAGCTGT+TGG + chr5_3:13660022-13660041 Msa0800300:CDS 50.0%
CACCAGCAATACTTTCCCCA+CGG + chr5_3:13660069-13660088 Msa0800300:CDS 50.0%
!! AACCGTGGGGAAAGTATTGC+TGG - chr5_3:13660074-13660093 None:intergenic 50.0%
GTTTGGGAAGAGGGTTTAGG+AGG + chr5_3:13660338-13660357 Msa0800300:intron 50.0%
TTGGGAAGAGGGTTTAGGAG+GGG + chr5_3:13660340-13660359 Msa0800300:intron 50.0%
AAGAGGGTTTAGGAGGGGAA+GGG + chr5_3:13660345-13660364 Msa0800300:intron 50.0%
AAGAGGGTTTGGGAAGGGAA+GGG - chr5_3:13660542-13660561 None:intergenic 50.0%
TTGGGAAGAGGGTTTGGGAA+GGG - chr5_3:13660547-13660566 None:intergenic 50.0%
TTTGGGAAGAGGGTTTGGGA+AGG - chr5_3:13660548-13660567 None:intergenic 50.0%
GTGTGTTTGGGAAGAGGGTT+TGG - chr5_3:13660334-13660353 None:intergenic 50.0%
ACCTGGTGTCCCCATAATTC+TGG + chr5_3:13661404-13661423 Msa0800300:CDS 50.0%
ACCAGAATTATGGGGACACC+AGG - chr5_3:13661408-13661427 None:intergenic 50.0%
GGTGTCCCCATAATTCTGGT+TGG + chr5_3:13661408-13661427 Msa0800300:CDS 50.0%
GAACTGGCTAACTGCTGAAC+CGG + chr5_3:13661608-13661627 Msa0800300:intron 50.0%
! TTTGTTTGCTAGGGGGAAGG+GGG + chr5_3:13661636-13661655 Msa0800300:intron 50.0%
CAGTTCTGCATAGACCATCC+TGG + chr5_3:13662000-13662019 Msa0800300:CDS 50.0%
!! CTGAGCTGTGGTAATGGGAA+CGG - chr5_3:13662028-13662047 None:intergenic 50.0%
AAAGAAGGGGAGGGGAATAC+AGG - chr5_3:13662433-13662452 None:intergenic 50.0%
GAAGAGGGTTTAGGAGGGGA+AGG + chr5_3:13660344-13660363 Msa0800300:intron 55.0%
GAAGAGGGTTTGGGAAGGGA+AGG - chr5_3:13660543-13660562 None:intergenic 55.0%
CTGGGTTTGTGGGATACTGC+TGG + chr5_3:13660997-13661016 Msa0800300:CDS 55.0%
TCGGCACCACGGTAACTCAA+AGG - chr5_3:13661137-13661156 None:intergenic 55.0%
! TTGTTTGCTAGGGGGAAGGG+GGG + chr5_3:13661637-13661656 Msa0800300:intron 55.0%
TAATGGGAACGGCACCAGGA+TGG - chr5_3:13662017-13662036 None:intergenic 55.0%
CGTTCCCATTACCACAGCTC+AGG + chr5_3:13662026-13662045 Msa0800300:CDS 55.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr5_3:13660348-13660367 Msa0800300:intron 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG + chr5_3:13660349-13660368 Msa0800300:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG + chr5_3:13660350-13660369 Msa0800300:intron 60.0%
GGTTTGGGAAGGGAAGGGAG+GGG - chr5_3:13660537-13660556 None:intergenic 60.0%
GGGTTTGGGAAGGGAAGGGA+GGG - chr5_3:13660538-13660557 None:intergenic 60.0%
AGGGTTTGGGAAGGGAAGGG+AGG - chr5_3:13660539-13660558 None:intergenic 60.0%
TAGGGGGAAGGGGGGATTTC+TGG + chr5_3:13661645-13661664 Msa0800300:intron 60.0%
!! GTGGTAATGGGAACGGCACC+AGG - chr5_3:13662021-13662040 None:intergenic 60.0%
GGAGGAGGGGAACTGTCCTC+AGG + chr5_3:13659930-13659949 Msa0800300:five_prime_UTR 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG + chr5_3:13660353-13660372 Msa0800300:intron 65.0%
TAGGAGGGGAAGGGAGGGGA+GGG + chr5_3:13660354-13660373 Msa0800300:intron 65.0%
TGGGAAGGGAAGGGAGGGGA+GGG - chr5_3:13660533-13660552 None:intergenic 65.0%
TTGGGAAGGGAAGGGAGGGG+AGG - chr5_3:13660534-13660553 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_3 gene 13659515 13662690 13659515 ID=Msa0800300;Name=Msa0800300
chr5_3 mRNA 13659515 13662690 13659515 ID=Msa0800300-mRNA-1;Parent=Msa0800300;Name=Msa0800300-mRNA-1;_AED=0.07;_eAED=0.07;_QI=315|1|1|1|0.85|0.75|8|298|197
chr5_3 exon 13659515 13659721 13659515 ID=Msa0800300-mRNA-1:exon:5633;Parent=Msa0800300-mRNA-1
chr5_3 exon 13659884 13660090 13659884 ID=Msa0800300-mRNA-1:exon:5634;Parent=Msa0800300-mRNA-1
chr5_3 exon 13660931 13661018 13660931 ID=Msa0800300-mRNA-1:exon:5635;Parent=Msa0800300-mRNA-1
chr5_3 exon 13661108 13661217 13661108 ID=Msa0800300-mRNA-1:exon:5636;Parent=Msa0800300-mRNA-1
chr5_3 exon 13661378 13661441 13661378 ID=Msa0800300-mRNA-1:exon:5637;Parent=Msa0800300-mRNA-1
chr5_3 exon 13661983 13662047 13661983 ID=Msa0800300-mRNA-1:exon:5638;Parent=Msa0800300-mRNA-1
chr5_3 exon 13662136 13662201 13662136 ID=Msa0800300-mRNA-1:exon:5639;Parent=Msa0800300-mRNA-1
chr5_3 exon 13662291 13662690 13662291 ID=Msa0800300-mRNA-1:exon:5640;Parent=Msa0800300-mRNA-1
chr5_3 five_prime_UTR 13659515 13659721 13659515 ID=Msa0800300-mRNA-1:five_prime_utr;Parent=Msa0800300-mRNA-1
chr5_3 five_prime_UTR 13659884 13659991 13659884 ID=Msa0800300-mRNA-1:five_prime_utr;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13659992 13660090 13659992 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13660931 13661018 13660931 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13661108 13661217 13661108 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13661378 13661441 13661378 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13661983 13662047 13661983 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13662136 13662201 13662136 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 CDS 13662291 13662392 13662291 ID=Msa0800300-mRNA-1:cds;Parent=Msa0800300-mRNA-1
chr5_3 three_prime_UTR 13662393 13662690 13662393 ID=Msa0800300-mRNA-1:three_prime_utr;Parent=Msa0800300-mRNA-1
Gene Sequence

>Msa0800300

ATGAGTGCTTCTAGGTTTATTAAATGTGTTACTGTTGGTGATGGAGCTGTTGGAAAAACTTGTTTGTTGATTTCTTACACCAGCAATACTTTCCCCACGGATTATGTGCCGACAGTTTTTGACAATTTCAGTGCGAATGTGGTTGTTAATGGAAGTATTGTGAATCTGGGTTTGTGGGATACTGCTGGGCAAGAGGATTATAACAGATTAAGACCTTTGAGTTACCGTGGTGCCGATGTTTTCATATTGGCTTTCTCTCTCATAAGCAAAGCCAGTTATGAAAATGTCTCCAAAAAGTGGATTCCAGAGTTGAAGCATTATGCACCTGGTGTCCCCATAATTCTGGTTGGAACAAAGCTTGACCTTCGGGATGATAAGCAGTTCTGCATAGACCATCCTGGTGCCGTTCCCATTACCACAGCTCAGGGAGAAGAGCTGAGGAAGCTGATTAATGCACCAGCTTACATTGAATGCAGTTCAAAATCACAGGAGAACGTGAAGGCGGTGTTTGACGCAGCCATAAGAGTTGTCCTTCAACCACCAAAGCAGAAGAAAAAGAAGAATAAAGCACAAAAGGCCTGTTCAATATTGTAA
Protein sequence

>Msa0800300

MSASRFIKCVTVGDGAVGKTCLLISYTSNTFPTDYVPTVFDNFSANVVVNGSIVNLGLWDTAGQEDYNRLRPLSYRGADVFILAFSLISKASYENVSKKWIPELKHYAPGVPIILVGTKLDLRDDKQFCIDHPGAVPITTAQGEELRKLINAPAYIECSSKSQENVKAVFDAAIRVVLQPPKQKKKKNKAQKACSIL*