AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0486400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0486400 MtrunA17_Chr3g0103011 94.361 266 15 0 1 266 1 266 0.0 534
Msa0486400 MtrunA17_Chr5g0444781 45.342 161 87 1 17 177 53 212 2.54e-45 157
Msa0486400 MtrunA17_Chr6g0483551 48.667 150 76 1 21 169 53 202 2.02e-42 146
Msa0486400 MtrunA17_Chr6g0483591 46.980 149 79 0 21 169 53 201 4.03e-41 142
Msa0486400 MtrunA17_Chr6g0483571 47.403 154 80 1 17 169 49 202 4.16e-41 142
Msa0486400 MtrunA17_Chr2g0290801 41.935 155 89 1 16 169 85 239 1.50e-34 126
Msa0486400 MtrunA17_Chr5g0444791 42.667 150 79 2 22 169 16 160 9.68e-32 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0486400 AT1G55790.1 59.091 154 63 0 16 169 63 216 2.14e-65 212
Msa0486400 AT4G26485.1 45.860 157 82 2 16 169 50 206 1.35e-39 139
Msa0486400 AT5G56060.1 45.638 149 77 3 15 161 5 151 9.12e-38 133
Msa0486400 AT1G55803.1 69.355 62 19 0 16 77 61 122 2.26e-27 104
Msa0486400 AT5G56075.1 38.854 157 76 3 17 169 110 250 4.18e-27 107
Msa0486400 AT5G25030.1 36.538 156 82 3 16 169 51 191 1.75e-25 101
Msa0486400 AT1G55800.1 53.571 84 39 0 86 169 11 94 2.23e-25 102
Msa0486400 AT2G11045.1 34.194 155 67 3 16 169 14 134 3.95e-16 74.7

Find 130 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATTTCTTAAATTGTTTA+TGG 0.116921 3_4:+4410647 None:intergenic
TCGCAACTTCGTGAAATTTA+AGG 0.134308 3_4:-4410790 Msa0486400:five_prime_UTR
CACATAGTCTCTCAAGTATT+TGG 0.136523 3_4:+4408750 None:intergenic
ATTTCACGAAGTTGCGATTT+TGG 0.149292 3_4:+4410796 None:intergenic
GTCCTCGACATTGTCATTTA+TGG 0.155281 3_4:-4410387 Msa0486400:five_prime_UTR
ACTATTAAAGCATTATAAAT+AGG 0.172562 3_4:+4411012 None:intergenic
GAAATAATGTTATACTATAA+TGG 0.203719 3_4:-4410900 Msa0486400:five_prime_UTR
CTTTCTATTGCAAAAGCTTT+TGG 0.255346 3_4:-4411090 Msa0486400:five_prime_UTR
ATCAAAAGTTTGAAATAAAA+AGG 0.260393 3_4:+4410291 None:intergenic
GCTTCTTCACTTGACACTTA+TGG 0.273879 3_4:-4411048 Msa0486400:five_prime_UTR
CAATTCTTCTAAATTTGATT+TGG 0.284080 3_4:+4409939 None:intergenic
AAGTCAAATTCTGATCTAAT+TGG 0.285572 3_4:+4408994 None:intergenic
AAATAATGTTATACTATAAT+GGG 0.300940 3_4:-4410899 Msa0486400:five_prime_UTR
GGATCCCTTGTTTGCCTACT+AGG 0.306423 3_4:-4408713 Msa0486400:three_prime_UTR
TGGTTGCTTTGAATGAAAAT+AGG 0.308725 3_4:-4410097 Msa0486400:five_prime_UTR
ATTCCTTTATGAATAAATAA+AGG 0.315842 3_4:+4410748 None:intergenic
TGTTGTATTAGTGTTGGTTT+AGG 0.317949 3_4:-4410589 Msa0486400:five_prime_UTR
TGAAGGAGAAGGGGATGTTA+TGG 0.320084 3_4:-4408881 Msa0486400:CDS
AAGCCTTTATTTATTCATAA+AGG 0.328016 3_4:-4410751 Msa0486400:five_prime_UTR
CTCTGAATCTCTTCATGAAA+TGG 0.328925 3_4:+4409135 None:intergenic
GGTTTAGGGTTTGAAGTTTA+CGG 0.330361 3_4:-4410574 Msa0486400:five_prime_UTR
CGCAACTTCGTGAAATTTAA+GGG 0.343963 3_4:-4410789 Msa0486400:five_prime_UTR
CTGTAGTGCCATTAGATATA+AGG 0.355231 3_4:-4408648 Msa0486400:three_prime_UTR
TCCAGCTCTTCTCCTCCACT+TGG 0.364659 3_4:+4408826 None:intergenic
CAGATTGAACACAAATTTAG+TGG 0.369546 3_4:+4410540 None:intergenic
GTTGTATTAGTGTTGGTTTA+GGG 0.369914 3_4:-4410588 Msa0486400:five_prime_UTR
TCAGAATTTGACTTGAGAAA+TGG 0.370628 3_4:-4408985 Msa0486400:CDS
TAGTAGCAACTTTCTTTGAT+TGG 0.376444 3_4:+4410427 None:intergenic
CTATTAAAGCATTATAAATA+GGG 0.378704 3_4:+4411013 None:intergenic
CCTCGTTTGTTGTTGTAACC+TGG 0.390809 3_4:+4409291 None:intergenic
CTATATGATTTCCCCTCAAA+TGG 0.396400 3_4:+4409184 None:intergenic
GTGAATTTAAAGGATGCATA+TGG 0.407557 3_4:-4409484 Msa0486400:intron
GGAAGATAGTTGAGATCAAC+TGG 0.409488 3_4:+4409093 None:intergenic
TTTAGAAGAATTGCAGAAGC+TGG 0.410090 3_4:-4409929 Msa0486400:CDS
CAATCATTGTCAGAAAGCTT+TGG 0.412205 3_4:+4409343 None:intergenic
GGGTCTGGTGCGGTTCCTCT+TGG 0.414919 3_4:+4408771 None:intergenic
ACATAGTCTCTCAAGTATTT+GGG 0.415278 3_4:+4408751 None:intergenic
TTTGTTTGTGTAGACAATTA+TGG 0.418283 3_4:-4410355 Msa0486400:five_prime_UTR
GTCTCTCAAGTATTTGGGTC+TGG 0.419801 3_4:+4408756 None:intergenic
GGTTTGAAGTTTACGGTTGA+AGG 0.420613 3_4:-4410567 Msa0486400:five_prime_UTR
CACTAAGGCCTTATATCTAA+TGG 0.420923 3_4:+4408640 None:intergenic
TCAAGTTGTCTTCCTTCCTA+TGG 0.423108 3_4:+4409800 None:intergenic
GAATGCATGTCAGATGCTTA+TGG 0.423497 3_4:-4409439 Msa0486400:CDS
CAAAGAAAGTTGCTACTACC+AGG 0.427859 3_4:-4410422 Msa0486400:five_prime_UTR
GCGGTTCCTCTTGGCCCGTA+TGG 0.428241 3_4:+4408780 None:intergenic
TGCTACAGTTGCAAAACTTA+GGG 0.429481 3_4:-4410850 Msa0486400:five_prime_UTR
TCGTTGTTTCTTTGAAATGA+AGG 0.434958 3_4:-4410712 Msa0486400:five_prime_UTR
ATCACCTCATCTACATGAAC+AGG 0.435847 3_4:+4409979 None:intergenic
CAAAATTGACCTTGTCCTCA+AGG 0.439623 3_4:+4408902 None:intergenic
CGGTTCCTCTTGGCCCGTAT+GGG 0.439724 3_4:+4408781 None:intergenic
CCCAAGTGGAGGAGAAGAGC+TGG 0.446261 3_4:-4408827 Msa0486400:CDS
TTCAACAAAGAAGATTATCC+AGG 0.446367 3_4:-4409309 Msa0486400:CDS
CTGCTACAGTTGCAAAACTT+AGG 0.451776 3_4:-4410851 Msa0486400:five_prime_UTR
CTGCACTTACCGAGAGGAAA+GGG 0.452267 3_4:+4409246 None:intergenic
AACAGTAATGAAATTTCATC+CGG 0.452991 3_4:-4409878 Msa0486400:CDS
CCATTCCTAGTAGGCAAACA+AGG 0.464092 3_4:+4408708 None:intergenic
ATGCATATGGATCTCGTGTT+CGG 0.467055 3_4:-4409471 Msa0486400:CDS
ACTTGGGTCAGCAAGTTCAA+TGG 0.467706 3_4:+4408843 None:intergenic
CTTGGGTCAGCAAGTTCAAT+GGG 0.471454 3_4:+4408844 None:intergenic
CCTTGTTTGCCTACTAGGAA+TGG 0.473495 3_4:-4408708 Msa0486400:three_prime_UTR
GTTTAAGTAGTGTGTCTCAA+TGG 0.482186 3_4:-4410200 Msa0486400:five_prime_UTR
ATATCTAATGGCACTACAGC+TGG 0.488920 3_4:+4408652 None:intergenic
ATATTCAACTTTCCTCATGC+AGG 0.489206 3_4:-4409826 Msa0486400:CDS
CATTGTCAGAAAGCTTTGGT+TGG 0.492728 3_4:+4409347 None:intergenic
TGATCATCAAATACTGTTAG+TGG 0.493099 3_4:-4411136 Msa0486400:five_prime_UTR
GTAGTGTGTCTCAATGGACT+TGG 0.495427 3_4:-4410194 Msa0486400:five_prime_UTR
TGTTGATGTTGTATTAGTGT+TGG 0.496792 3_4:-4410595 Msa0486400:five_prime_UTR
AGACCTTATCCATTCCTAGT+AGG 0.500939 3_4:+4408699 None:intergenic
CATTAGATATAAGGCCTTAG+TGG 0.506734 3_4:-4408639 Msa0486400:three_prime_UTR
TCTGCAAAATCAAAATGCTC+TGG 0.507034 3_4:-4410026 Msa0486400:exon
AGTGCATGTAAGAAGAAACT+AGG 0.508831 3_4:-4411207 Msa0486400:five_prime_UTR
TTGCCTACTAGGAATGGATA+AGG 0.510045 3_4:-4408702 Msa0486400:three_prime_UTR
CTACACCTCCTTTCAATGAC+TGG 0.510312 3_4:-4409386 Msa0486400:CDS
AGGAATGGATAAGGTCTTAG+TGG 0.511022 3_4:-4408693 Msa0486400:three_prime_UTR
CTGGGAGCATACGTGTTACA+TGG 0.511436 3_4:-4409910 Msa0486400:CDS
AAACTATACCATATTAGATA+AGG 0.512810 3_4:+4410130 None:intergenic
TAGTTGAGATCAACTGGAGC+TGG 0.515891 3_4:+4409099 None:intergenic
TTTGGAAATAAACTTGTTCG+AGG 0.521347 3_4:+4409072 None:intergenic
TTAGAAGAATTGCAGAAGCT+GGG 0.525297 3_4:-4409928 Msa0486400:CDS
TATAATATAATGTTCCACTA+AGG 0.528049 3_4:+4408625 None:intergenic
ATGGATACAATCCGATCTCA+AGG 0.528394 3_4:-4408966 Msa0486400:CDS
CCAGCTCTTCTCCTCCACTT+GGG 0.530467 3_4:+4408827 None:intergenic
GAGAAGAGCTGGAAGAAGAC+AGG 0.532441 3_4:-4408816 Msa0486400:CDS
TTGTTGATCTCCCTTGAGAT+CGG 0.536772 3_4:+4408955 None:intergenic
AGTAATGTGGCAAAATTTGA+CGG 0.537095 3_4:+4410048 None:intergenic
TTCTTTGAAATGAAGGTGCA+TGG 0.541482 3_4:-4410705 Msa0486400:five_prime_UTR
CAAATACTGTTAGTGGGTGA+TGG 0.554164 3_4:-4411129 Msa0486400:five_prime_UTR
TGTGATGACCCCTTTCCTCT+CGG 0.554223 3_4:-4409255 Msa0486400:CDS
GGATCCATGTTACTCCCATA+CGG 0.560176 3_4:-4408795 Msa0486400:CDS
CAAATACTTGAGAGACTATG+TGG 0.561291 3_4:-4408749 Msa0486400:CDS
GATCATCAAATACTGTTAGT+GGG 0.567279 3_4:-4411135 Msa0486400:five_prime_UTR
CTATGTGGATCAGTAAGCAC+AGG 0.573416 3_4:-4408734 Msa0486400:exon
TCATGCAGGTTTCCATAGGA+AGG 0.577731 3_4:-4409812 Msa0486400:CDS
TTTAGAAAGTGATTTGTCTG+AGG 0.579433 3_4:+4409027 None:intergenic
ACTTCTGAACCTTGAGGACA+AGG 0.580193 3_4:-4408911 Msa0486400:CDS
GCTGCACTTACCGAGAGGAA+AGG 0.583209 3_4:+4409245 None:intergenic
TCAAGTATTTGGGTCTGGTG+CGG 0.585653 3_4:+4408761 None:intergenic
TTCCTCATGCAGGTTTCCAT+AGG 0.587250 3_4:-4409816 Msa0486400:CDS
TGACAATGTCGAGGACTCGA+GGG 0.588556 3_4:+4410394 None:intergenic
TCAATGCTCCAGTCATTGAA+AGG 0.589310 3_4:+4409378 None:intergenic
ATGACAATGTCGAGGACTCG+AGG 0.589338 3_4:+4410393 None:intergenic
GTGCATGTAAGAAGAAACTA+GGG 0.590755 3_4:-4411206 Msa0486400:five_prime_UTR
AAAGAAAGTTGCTACTACCA+GGG 0.597849 3_4:-4410421 Msa0486400:five_prime_UTR
AGCATACGTGTTACATGGAG+TGG 0.600625 3_4:-4409905 Msa0486400:CDS
ACCTCATCTACATGAACAGG+AGG 0.608114 3_4:+4409982 None:intergenic
TCTAATGGCACTACAGCTGG+TGG 0.609934 3_4:+4408655 None:intergenic
GATCCATGTTACTCCCATAC+GGG 0.611469 3_4:-4408794 Msa0486400:CDS
TGAAATACTTCTGAACCTTG+AGG 0.612747 3_4:-4408917 Msa0486400:CDS
GCAACTTCGTGAAATTTAAG+GGG 0.613979 3_4:-4410788 Msa0486400:five_prime_UTR
CCCTCCTGTTCATGTAGATG+AGG 0.616310 3_4:-4409983 Msa0486400:CDS
ATTGACATGAATCTCACCAT+TGG 0.626240 3_4:+4409416 None:intergenic
TGGCCCGTATGGGAGTAACA+TGG 0.633048 3_4:+4408791 None:intergenic
GCTACAGTTGCAAAACTTAG+GGG 0.634426 3_4:-4410849 Msa0486400:five_prime_UTR
TTGTGTTCAATCTGAAGTGT+CGG 0.635703 3_4:-4410531 Msa0486400:five_prime_UTR
CATTCCTAGTAGGCAAACAA+GGG 0.636597 3_4:+4408709 None:intergenic
AACCATAAATGACAATGTCG+AGG 0.641431 3_4:+4410385 None:intergenic
GAACTTGCTGACCCAAGTGG+AGG 0.643038 3_4:-4408838 Msa0486400:CDS
TGTCAGATGCTTATGGCCAA+TGG 0.648335 3_4:-4409432 Msa0486400:CDS
TGGATACAATCCGATCTCAA+GGG 0.656266 3_4:-4408965 Msa0486400:CDS
ATTGAACTTGCTGACCCAAG+TGG 0.657087 3_4:-4408841 Msa0486400:CDS
CTACAGTTGCAAAACTTAGG+GGG 0.657466 3_4:-4410848 Msa0486400:five_prime_UTR
TGCACTTACCGAGAGGAAAG+GGG 0.664703 3_4:+4409247 None:intergenic
TTCCTATGGAAACCTGCATG+AGG 0.665648 3_4:+4409814 None:intergenic
CCAGGTTACAACAACAAACG+AGG 0.674784 3_4:-4409291 Msa0486400:CDS
ATGCTCCAGTCATTGAAAGG+AGG 0.678716 3_4:+4409381 None:intergenic
TTACTCCCATACGGGCCAAG+AGG 0.679246 3_4:-4408786 Msa0486400:CDS
GTGCATGTGAATGTAATGCA+TGG 0.711013 3_4:-4410239 Msa0486400:five_prime_UTR
GAGGACTCGAGGGTTAACCC+TGG 0.717253 3_4:+4410404 None:intergenic
CCTCATCTACATGAACAGGA+GGG 0.718871 3_4:+4409983 None:intergenic
AAAGTGCTGCACTTACCGAG+AGG 0.719258 3_4:+4409240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATTTTGATATTTGAAT+TGG - chr3_4:4408801-4408820 Msa0486400:CDS 10.0%
!! AAATAATGTTATACTATAAT+GGG - chr3_4:4408844-4408863 Msa0486400:CDS 10.0%
!!! TAATTAACATATTTCTAAAT+AGG + chr3_4:4410167-4410186 None:intergenic 10.0%
!!! CTATTAAAGCATTATAAATA+GGG + chr3_4:4408733-4408752 None:intergenic 15.0%
!!! ACTATTAAAGCATTATAAAT+AGG + chr3_4:4408734-4408753 None:intergenic 15.0%
!! GAAATAATGTTATACTATAA+TGG - chr3_4:4408843-4408862 Msa0486400:CDS 15.0%
!!! TTTTGTAGTTTTTTTTTCTT+AGG - chr3_4:4408921-4408940 Msa0486400:CDS 15.0%
!!! TTTGTAGTTTTTTTTTCTTA+GGG - chr3_4:4408922-4408941 Msa0486400:CDS 15.0%
!! ATTCCTTTATGAATAAATAA+AGG + chr3_4:4408998-4409017 None:intergenic 15.0%
!! CAAATTTCTTAAATTGTTTA+TGG + chr3_4:4409099-4409118 None:intergenic 15.0%
!!! ATCAAAAGTTTGAAATAAAA+AGG + chr3_4:4409455-4409474 None:intergenic 15.0%
!! AGAAATATGTTAATTATCTT+TGG - chr3_4:4410171-4410190 Msa0486400:five_prime_UTR 15.0%
!!! ATTTCTTTAAGAAAAAGAAA+AGG - chr3_4:4411223-4411242 Msa0486400:five_prime_UTR 15.0%
!! TTTAACACTAAACGAAAAAA+GGG + chr3_4:4408785-4408804 None:intergenic 20.0%
!!! TTTTAACACTAAACGAAAAA+AGG + chr3_4:4408786-4408805 None:intergenic 20.0%
!!! TTGTAGTTTTTTTTTCTTAG+GGG - chr3_4:4408923-4408942 Msa0486400:CDS 20.0%
!!! AAGCCTTTATTTATTCATAA+AGG - chr3_4:4408992-4409011 Msa0486400:CDS 20.0%
!!! GTGTTTTTGAATATGATAAA+AGG - chr3_4:4409263-4409282 Msa0486400:CDS 20.0%
!!! GTTTTTTTCCTTATCTAATA+TGG - chr3_4:4409605-4409624 Msa0486400:intron 20.0%
!! AAACTATACCATATTAGATA+AGG + chr3_4:4409616-4409635 None:intergenic 20.0%
!! CAATTCTTCTAAATTTGATT+TGG + chr3_4:4409807-4409826 None:intergenic 20.0%
!! ACTTGTGCATAATAATAAAA+GGG + chr3_4:4410123-4410142 None:intergenic 20.0%
!! AACTTGTGCATAATAATAAA+AGG + chr3_4:4410124-4410143 None:intergenic 20.0%
!!! CAATTGAATTGATTCATTTT+TGG + chr3_4:4410692-4410711 None:intergenic 20.0%
!! TATAATATAATGTTCCACTA+AGG + chr3_4:4411121-4411140 None:intergenic 20.0%
!!! TGTAGTTTTTTTTTCTTAGG+GGG - chr3_4:4408924-4408943 Msa0486400:CDS 25.0%
!!! ATGAGTATCATTTTTATAGC+TGG + chr3_4:4409069-4409088 None:intergenic 25.0%
! TTTGTTTGTGTAGACAATTA+TGG - chr3_4:4409388-4409407 Msa0486400:CDS 25.0%
!! TTCCTATTGTTCACAATTTT+TGG - chr3_4:4409479-4409498 Msa0486400:intron 25.0%
!! TCCTATTGTTCACAATTTTT+GGG - chr3_4:4409480-4409499 Msa0486400:intron 25.0%
!!! CTAATATGGTATAGTTTTGA+AGG - chr3_4:4409619-4409638 Msa0486400:intron 25.0%
! AACAGTAATGAAATTTCATC+CGG - chr3_4:4409865-4409884 Msa0486400:CDS 25.0%
!! TTGAGTACTTTTAAACACTT+CGG - chr3_4:4410022-4410041 Msa0486400:exon 25.0%
! CTTGTGCATAATAATAAAAG+GGG + chr3_4:4410122-4410141 None:intergenic 25.0%
! ATCTTTGGTTATCATAGTTA+AGG - chr3_4:4410186-4410205 Msa0486400:five_prime_UTR 25.0%
!! AAATGGTTTTTCATACTTCT+AGG + chr3_4:4410545-4410564 None:intergenic 25.0%
! AAGTATGAAAAACCATTTGA+GGG - chr3_4:4410547-4410566 Msa0486400:five_prime_UTR 25.0%
! AAGTCAAATTCTGATCTAAT+TGG + chr3_4:4410752-4410771 None:intergenic 25.0%
!! CTTTTCTTCTTTGATTTTCG+TGG + chr3_4:4408518-4408537 None:intergenic 30.0%
AGCAACAACAACAACAAAAA+TGG - chr3_4:4408567-4408586 Msa0486400:three_prime_UTR 30.0%
TGATCATCAAATACTGTTAG+TGG - chr3_4:4408607-4408626 Msa0486400:three_prime_UTR 30.0%
GATCATCAAATACTGTTAGT+GGG - chr3_4:4408608-4408627 Msa0486400:three_prime_UTR 30.0%
! CTTTCTATTGCAAAAGCTTT+TGG - chr3_4:4408653-4408672 Msa0486400:three_prime_UTR 30.0%
!!! TTGGATGCATGTTTTTTAGT+TGG - chr3_4:4408820-4408839 Msa0486400:CDS 30.0%
!!! TGGATGCATGTTTTTTAGTT+GGG - chr3_4:4408821-4408840 Msa0486400:CDS 30.0%
!! TCGTTGTTTCTTTGAAATGA+AGG - chr3_4:4409031-4409050 Msa0486400:CDS 30.0%
TGTTGATGTTGTATTAGTGT+TGG - chr3_4:4409148-4409167 Msa0486400:CDS 30.0%
!! TGTTGTATTAGTGTTGGTTT+AGG - chr3_4:4409154-4409173 Msa0486400:CDS 30.0%
!! GTTGTATTAGTGTTGGTTTA+GGG - chr3_4:4409155-4409174 Msa0486400:CDS 30.0%
CAGATTGAACACAAATTTAG+TGG + chr3_4:4409206-4409225 None:intergenic 30.0%
TAGTAGCAACTTTCTTTGAT+TGG + chr3_4:4409319-4409338 None:intergenic 30.0%
TCCCAAAAATTGTGAACAAT+AGG + chr3_4:4409484-4409503 None:intergenic 30.0%
! TGGTTGCTTTGAATGAAAAT+AGG - chr3_4:4409646-4409665 Msa0486400:intron 30.0%
AGTAATGTGGCAAAATTTGA+CGG + chr3_4:4409698-4409717 None:intergenic 30.0%
!! TTTGATTTTGCAGAGTAATG+TGG + chr3_4:4409711-4409730 None:intergenic 30.0%
! AACCTCTGCATTTTTAAATC+CGG + chr3_4:4409887-4409906 None:intergenic 30.0%
GTGTTTCCTTGTGAATTTAA+AGG - chr3_4:4410249-4410268 Msa0486400:five_prime_UTR 30.0%
ATGCATCCTTTAAATTCACA+AGG + chr3_4:4410258-4410277 None:intergenic 30.0%
GTGAATTTAAAGGATGCATA+TGG - chr3_4:4410259-4410278 Msa0486400:five_prime_UTR 30.0%
TTCAACAAAGAAGATTATCC+AGG - chr3_4:4410434-4410453 Msa0486400:five_prime_UTR 30.0%
GAAGTATGAAAAACCATTTG+AGG - chr3_4:4410546-4410565 Msa0486400:five_prime_UTR 30.0%
AGTATGAAAAACCATTTGAG+GGG - chr3_4:4410548-4410567 Msa0486400:five_prime_UTR 30.0%
! TTTGGAAATAAACTTGTTCG+AGG + chr3_4:4410674-4410693 None:intergenic 30.0%
! TTTAGAAAGTGATTTGTCTG+AGG + chr3_4:4410719-4410738 None:intergenic 30.0%
!! TCAGAATTTGACTTGAGAAA+TGG - chr3_4:4410758-4410777 Msa0486400:five_prime_UTR 30.0%
ACATAGTCTCTCAAGTATTT+GGG + chr3_4:4410995-4411014 None:intergenic 30.0%
AGTGCATGTAAGAAGAAACT+AGG - chr3_4:4408536-4408555 Msa0486400:three_prime_UTR 35.0%
GTGCATGTAAGAAGAAACTA+GGG - chr3_4:4408537-4408556 Msa0486400:three_prime_UTR 35.0%
TGCTACAGTTGCAAAACTTA+GGG - chr3_4:4408893-4408912 Msa0486400:CDS 35.0%
! ATTTCACGAAGTTGCGATTT+TGG + chr3_4:4408950-4408969 None:intergenic 35.0%
TCGCAACTTCGTGAAATTTA+AGG - chr3_4:4408953-4408972 Msa0486400:CDS 35.0%
CGCAACTTCGTGAAATTTAA+GGG - chr3_4:4408954-4408973 Msa0486400:CDS 35.0%
GCAACTTCGTGAAATTTAAG+GGG - chr3_4:4408955-4408974 Msa0486400:CDS 35.0%
!! TTCTTTGAAATGAAGGTGCA+TGG - chr3_4:4409038-4409057 Msa0486400:CDS 35.0%
! GGTTTAGGGTTTGAAGTTTA+CGG - chr3_4:4409169-4409188 Msa0486400:CDS 35.0%
! TTGTGTTCAATCTGAAGTGT+CGG - chr3_4:4409212-4409231 Msa0486400:CDS 35.0%
AAAGAAAGTTGCTACTACCA+GGG - chr3_4:4409322-4409341 Msa0486400:CDS 35.0%
AACCATAAATGACAATGTCG+AGG + chr3_4:4409361-4409380 None:intergenic 35.0%
!!! GCATGGTGATGTATTTTGAT+TGG - chr3_4:4409521-4409540 Msa0486400:intron 35.0%
GTTTAAGTAGTGTGTCTCAA+TGG - chr3_4:4409543-4409562 Msa0486400:intron 35.0%
TCTGCAAAATCAAAATGCTC+TGG - chr3_4:4409717-4409736 Msa0486400:intron 35.0%
TTTAGAAGAATTGCAGAAGC+TGG - chr3_4:4409814-4409833 Msa0486400:CDS 35.0%
TTAGAAGAATTGCAGAAGCT+GGG - chr3_4:4409815-4409834 Msa0486400:CDS 35.0%
ATCCGGATTTAAAAATGCAG+AGG - chr3_4:4409882-4409901 Msa0486400:CDS 35.0%
ATATTCAACTTTCCTCATGC+AGG - chr3_4:4409917-4409936 Msa0486400:CDS 35.0%
! TACTTTTAAACACTTCGGAG+TGG - chr3_4:4410027-4410046 Msa0486400:exon 35.0%
ATTGACATGAATCTCACCAT+TGG + chr3_4:4410330-4410349 None:intergenic 35.0%
CAATCATTGTCAGAAAGCTT+TGG + chr3_4:4410403-4410422 None:intergenic 35.0%
CTATATGATTTCCCCTCAAA+TGG + chr3_4:4410562-4410581 None:intergenic 35.0%
CTCTGAATCTCTTCATGAAA+TGG + chr3_4:4410611-4410630 None:intergenic 35.0%
TGAAATACTTCTGAACCTTG+AGG - chr3_4:4410826-4410845 Msa0486400:five_prime_UTR 35.0%
!! AAGGTCAATTTTGAAGGAGA+AGG - chr3_4:4410851-4410870 Msa0486400:five_prime_UTR 35.0%
!! AGGTCAATTTTGAAGGAGAA+GGG - chr3_4:4410852-4410871 Msa0486400:five_prime_UTR 35.0%
CACATAGTCTCTCAAGTATT+TGG + chr3_4:4410996-4411015 None:intergenic 35.0%
CAAATACTTGAGAGACTATG+TGG - chr3_4:4410994-4411013 Msa0486400:five_prime_UTR 35.0%
! CTGTAGTGCCATTAGATATA+AGG - chr3_4:4411095-4411114 Msa0486400:five_prime_UTR 35.0%
! CACTAAGGCCTTATATCTAA+TGG + chr3_4:4411106-4411125 None:intergenic 35.0%
CATTAGATATAAGGCCTTAG+TGG - chr3_4:4411104-4411123 Msa0486400:five_prime_UTR 35.0%
! CAAATACTGTTAGTGGGTGA+TGG - chr3_4:4408614-4408633 Msa0486400:three_prime_UTR 40.0%
GCTTCTTCACTTGACACTTA+TGG - chr3_4:4408695-4408714 Msa0486400:three_prime_UTR 40.0%
CTGCTACAGTTGCAAAACTT+AGG - chr3_4:4408892-4408911 Msa0486400:CDS 40.0%
GCTACAGTTGCAAAACTTAG+GGG - chr3_4:4408894-4408913 Msa0486400:CDS 40.0%
CTACAGTTGCAAAACTTAGG+GGG - chr3_4:4408895-4408914 Msa0486400:CDS 40.0%
! GGTTTGAAGTTTACGGTTGA+AGG - chr3_4:4409176-4409195 Msa0486400:CDS 40.0%
CAAAGAAAGTTGCTACTACC+AGG - chr3_4:4409321-4409340 Msa0486400:CDS 40.0%
! GTCCTCGACATTGTCATTTA+TGG - chr3_4:4409356-4409375 Msa0486400:CDS 40.0%
GTGCATGTGAATGTAATGCA+TGG - chr3_4:4409504-4409523 Msa0486400:intron 40.0%
!!! GGTATAGTTTTGAAGGTGCT+TGG - chr3_4:4409626-4409645 Msa0486400:intron 40.0%
ATCACCTCATCTACATGAAC+AGG + chr3_4:4409767-4409786 None:intergenic 40.0%
! TCAAGTTGTCTTCCTTCCTA+TGG + chr3_4:4409946-4409965 None:intergenic 40.0%
ATGCATATGGATCTCGTGTT+CGG - chr3_4:4410272-4410291 Msa0486400:five_prime_UTR 40.0%
GAATGCATGTCAGATGCTTA+TGG - chr3_4:4410304-4410323 Msa0486400:five_prime_UTR 40.0%
TCAATGCTCCAGTCATTGAA+AGG + chr3_4:4410368-4410387 None:intergenic 40.0%
! CATTGTCAGAAAGCTTTGGT+TGG + chr3_4:4410399-4410418 None:intergenic 40.0%
GGAAGATAGTTGAGATCAAC+TGG + chr3_4:4410653-4410672 None:intergenic 40.0%
ATGGATACAATCCGATCTCA+AGG - chr3_4:4410777-4410796 Msa0486400:five_prime_UTR 40.0%
TGGATACAATCCGATCTCAA+GGG - chr3_4:4410778-4410797 Msa0486400:five_prime_UTR 40.0%
TTGTTGATCTCCCTTGAGAT+CGG + chr3_4:4410791-4410810 None:intergenic 40.0%
CAAAATTGACCTTGTCCTCA+AGG + chr3_4:4410844-4410863 None:intergenic 40.0%
! GAGGACAAGGTCAATTTTGA+AGG - chr3_4:4410845-4410864 Msa0486400:five_prime_UTR 40.0%
!! GGTCAATTTTGAAGGAGAAG+GGG - chr3_4:4410853-4410872 Msa0486400:five_prime_UTR 40.0%
CATTCCTAGTAGGCAAACAA+GGG + chr3_4:4411037-4411056 None:intergenic 40.0%
TTGCCTACTAGGAATGGATA+AGG - chr3_4:4411041-4411060 Msa0486400:five_prime_UTR 40.0%
AGACCTTATCCATTCCTAGT+AGG + chr3_4:4411047-4411066 None:intergenic 40.0%
AGGAATGGATAAGGTCTTAG+TGG - chr3_4:4411050-4411069 Msa0486400:five_prime_UTR 40.0%
ATATCTAATGGCACTACAGC+TGG + chr3_4:4411094-4411113 None:intergenic 40.0%
! GTAGTGTGTCTCAATGGACT+TGG - chr3_4:4409549-4409568 Msa0486400:intron 45.0%
CCTCATCTACATGAACAGGA+GGG + chr3_4:4409763-4409782 None:intergenic 45.0%
ACCTCATCTACATGAACAGG+AGG + chr3_4:4409764-4409783 None:intergenic 45.0%
AGCATACGTGTTACATGGAG+TGG - chr3_4:4409838-4409857 Msa0486400:CDS 45.0%
TTCCTCATGCAGGTTTCCAT+AGG - chr3_4:4409927-4409946 Msa0486400:CDS 45.0%
TTCCTATGGAAACCTGCATG+AGG + chr3_4:4409932-4409951 None:intergenic 45.0%
TCATGCAGGTTTCCATAGGA+AGG - chr3_4:4409931-4409950 Msa0486400:CDS 45.0%
TGTCAGATGCTTATGGCCAA+TGG - chr3_4:4410311-4410330 Msa0486400:five_prime_UTR 45.0%
CTACACCTCCTTTCAATGAC+TGG - chr3_4:4410357-4410376 Msa0486400:five_prime_UTR 45.0%
ATGCTCCAGTCATTGAAAGG+AGG + chr3_4:4410365-4410384 None:intergenic 45.0%
!! CCTCGTTTGTTGTTGTAACC+TGG + chr3_4:4410455-4410474 None:intergenic 45.0%
CCAGGTTACAACAACAAACG+AGG - chr3_4:4410452-4410471 Msa0486400:five_prime_UTR 45.0%
TAGTTGAGATCAACTGGAGC+TGG + chr3_4:4410647-4410666 None:intergenic 45.0%
ACTTCTGAACCTTGAGGACA+AGG - chr3_4:4410832-4410851 Msa0486400:five_prime_UTR 45.0%
TGAAGGAGAAGGGGATGTTA+TGG - chr3_4:4410862-4410881 Msa0486400:five_prime_UTR 45.0%
CTTGGGTCAGCAAGTTCAAT+GGG + chr3_4:4410902-4410921 None:intergenic 45.0%
ACTTGGGTCAGCAAGTTCAA+TGG + chr3_4:4410903-4410922 None:intergenic 45.0%
!! ATTGAACTTGCTGACCCAAG+TGG - chr3_4:4410902-4410921 Msa0486400:five_prime_UTR 45.0%
GGATCCATGTTACTCCCATA+CGG - chr3_4:4410948-4410967 Msa0486400:five_prime_UTR 45.0%
GATCCATGTTACTCCCATAC+GGG - chr3_4:4410949-4410968 Msa0486400:five_prime_UTR 45.0%
TCAAGTATTTGGGTCTGGTG+CGG + chr3_4:4410985-4411004 None:intergenic 45.0%
GTCTCTCAAGTATTTGGGTC+TGG + chr3_4:4410990-4411009 None:intergenic 45.0%
CTATGTGGATCAGTAAGCAC+AGG - chr3_4:4411009-4411028 Msa0486400:five_prime_UTR 45.0%
CCATTCCTAGTAGGCAAACA+AGG + chr3_4:4411038-4411057 None:intergenic 45.0%
! CCTTGTTTGCCTACTAGGAA+TGG - chr3_4:4411035-4411054 Msa0486400:five_prime_UTR 45.0%
!!! ATAGTTATTTTTTTTTTTAT+CGG - chr3_4:4409241-4409260 Msa0486400:CDS 5.0%
CAGTTGCAAAACTTAGGGGG+CGG - chr3_4:4408898-4408917 Msa0486400:CDS 50.0%
TGACAATGTCGAGGACTCGA+GGG + chr3_4:4409352-4409371 None:intergenic 50.0%
ATGACAATGTCGAGGACTCG+AGG + chr3_4:4409353-4409372 None:intergenic 50.0%
CCCTCCTGTTCATGTAGATG+AGG - chr3_4:4409760-4409779 Msa0486400:intron 50.0%
! CTGGGAGCATACGTGTTACA+TGG - chr3_4:4409833-4409852 Msa0486400:CDS 50.0%
TGTGATGACCCCTTTCCTCT+CGG - chr3_4:4410488-4410507 Msa0486400:five_prime_UTR 50.0%
TGCACTTACCGAGAGGAAAG+GGG + chr3_4:4410499-4410518 None:intergenic 50.0%
CTGCACTTACCGAGAGGAAA+GGG + chr3_4:4410500-4410519 None:intergenic 50.0%
!! AAAGTGCTGCACTTACCGAG+AGG + chr3_4:4410506-4410525 None:intergenic 50.0%
GAGAAGAGCTGGAAGAAGAC+AGG - chr3_4:4410927-4410946 Msa0486400:five_prime_UTR 50.0%
! GGATCCCTTGTTTGCCTACT+AGG - chr3_4:4411030-4411049 Msa0486400:five_prime_UTR 50.0%
TCTAATGGCACTACAGCTGG+TGG + chr3_4:4411091-4411110 None:intergenic 50.0%
GCTGCACTTACCGAGAGGAA+AGG + chr3_4:4410501-4410520 None:intergenic 55.0%
!! GAACTTGCTGACCCAAGTGG+AGG - chr3_4:4410905-4410924 Msa0486400:five_prime_UTR 55.0%
CCAGCTCTTCTCCTCCACTT+GGG + chr3_4:4410919-4410938 None:intergenic 55.0%
TCCAGCTCTTCTCCTCCACT+TGG + chr3_4:4410920-4410939 None:intergenic 55.0%
TGGCCCGTATGGGAGTAACA+TGG + chr3_4:4410955-4410974 None:intergenic 55.0%
TTACTCCCATACGGGCCAAG+AGG - chr3_4:4410957-4410976 Msa0486400:five_prime_UTR 55.0%
GAGGACTCGAGGGTTAACCC+TGG + chr3_4:4409342-4409361 None:intergenic 60.0%
CCCAAGTGGAGGAGAAGAGC+TGG - chr3_4:4410916-4410935 Msa0486400:five_prime_UTR 60.0%
CGGTTCCTCTTGGCCCGTAT+GGG + chr3_4:4410965-4410984 None:intergenic 60.0%
GCGGTTCCTCTTGGCCCGTA+TGG + chr3_4:4410966-4410985 None:intergenic 65.0%
GGGTCTGGTGCGGTTCCTCT+TGG + chr3_4:4410975-4410994 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 4408514 4411251 4408514 ID=Msa0486400;Name=Msa0486400
chr3_4 mRNA 4408514 4411251 4408514 ID=Msa0486400-mRNA-1;Parent=Msa0486400;Name=Msa0486400-mRNA-1;_AED=0.29;_eAED=0.29;_QI=1217|1|1|1|1|1|2|227|332
chr3_4 exon 4408514 4409494 4408514 ID=Msa0486400-mRNA-1:exon:1662;Parent=Msa0486400-mRNA-1
chr3_4 exon 4409790 4411251 4409790 ID=Msa0486400-mRNA-1:exon:1661;Parent=Msa0486400-mRNA-1
chr3_4 five_prime_UTR 4410035 4411251 4410035 ID=Msa0486400-mRNA-1:five_prime_utr;Parent=Msa0486400-mRNA-1
chr3_4 CDS 4409790 4410034 4409790 ID=Msa0486400-mRNA-1:cds;Parent=Msa0486400-mRNA-1
chr3_4 CDS 4408741 4409494 4408741 ID=Msa0486400-mRNA-1:cds;Parent=Msa0486400-mRNA-1
chr3_4 three_prime_UTR 4408514 4408740 4408514 ID=Msa0486400-mRNA-1:three_prime_utr;Parent=Msa0486400-mRNA-1
Gene Sequence

>Msa0486400

ATGCTCTGGTCTTGCTTTTGTCATCGATGCCCTCCTGTTCATGTAGATGAGGTGATCAAGAAGTACAAAAATGCCAAATCAAATTTAGAAGAATTGCAGAAGCTGGGAGCATACGTGTTACATGGAGTGGATGCAACAGTAATGAAATTTCATCCGGATTTAAAAATGCAGAGGTTTGATCGCATTATATTCAACTTTCCTCATGCAGGTTTCCATAGGAAGGAAGACAACTTGATGATGATCATGATGCATATGGATCTCGTGTTCGGTTTCTTCAAGAATGCATGTCAGATGCTTATGGCCAATGGTGAGATTCATGTCAATCACAAAACTACACCTCCTTTCAATGACTGGAGCATTGAGAAGCTCGCCAACCAAAGCTTTCTGACAATGATTGACTGCATTGATTTCAACAAAGAAGATTATCCAGGTTACAACAACAAACGAGGAGATAGCTATCGATGTGATGACCCCTTTCCTCTCGGTAAGTGCAGCACTTTCAAGTTCATATGCAATCCTAGAAGTATGAAAAACCATTTGAGGGGAAATCATATAGAGATTTCTAGACAGCAGACAATTCTTCCATTTCATGAAGAGATTCAGAGCATTGCGCAATTTCCAGCTCCAGTTGATCTCAACTATCTTCCTCGAACAAGTTTATTTCCAAAAATGAATCAATTCAATTGTTATCCTCAGACAAATCACTTTCTAAAGTTGAATGAACCAATTAGATCAGAATTTGACTTGAGAAATGGATACAATCCGATCTCAAGGGAGATCAACAATTTGACAGAAATACTTGAAATACTTCTGAACCTTGAGGACAAGGTCAATTTTGAAGGAGAAGGGGATGTTATGGATCACAACATTGAACCCATTGAACTTGCTGACCCAAGTGGAGGAGAAGAGCTGGAAGAAGACAGGATCCATGTTACTCCCATACGGGCCAAGAGGAACCGCACCAGACCCAAATACTTGAGAGACTATGTGGATCAGTAA
Protein sequence

>Msa0486400

MLWSCFCHRCPPVHVDEVIKKYKNAKSNLEELQKLGAYVLHGVDATVMKFHPDLKMQRFDRIIFNFPHAGFHRKEDNLMMIMMHMDLVFGFFKNACQMLMANGEIHVNHKTTPPFNDWSIEKLANQSFLTMIDCIDFNKEDYPGYNNKRGDSYRCDDPFPLGKCSTFKFICNPRSMKNHLRGNHIEISRQQTILPFHEEIQSIAQFPAPVDLNYLPRTSLFPKMNQFNCYPQTNHFLKLNEPIRSEFDLRNGYNPISREINNLTEILEILLNLEDKVNFEGEGDVMDHNIEPIELADPSGGEELEEDRIHVTPIRAKRNRTRPKYLRDYVDQ*