AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1144370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144370 MtrunA17_Chr3g0103011 93.609 266 17 0 1 266 1 266 0.0 531
Msa1144370 MtrunA17_Chr5g0444781 44.720 161 88 1 17 177 53 212 3.64e-45 156
Msa1144370 MtrunA17_Chr6g0483551 47.742 155 80 1 16 169 48 202 1.68e-43 149
Msa1144370 MtrunA17_Chr6g0483571 48.701 154 78 1 17 169 49 202 3.28e-43 148
Msa1144370 MtrunA17_Chr6g0483591 46.053 152 82 0 18 169 50 201 2.60e-41 143
Msa1144370 MtrunA17_Chr2g0290801 41.935 155 89 1 16 169 85 239 1.27e-35 129
Msa1144370 MtrunA17_Chr5g0444791 42.667 150 79 2 22 169 16 160 2.48e-32 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144370 AT1G55790.1 60.390 154 61 0 16 169 63 216 1.68e-66 214
Msa1144370 AT4G26485.1 45.860 157 82 2 16 169 50 206 3.47e-40 140
Msa1144370 AT5G56060.1 45.638 149 77 3 15 161 5 151 1.29e-38 135
Msa1144370 AT5G56075.1 38.854 157 76 3 17 169 110 250 8.72e-28 109
Msa1144370 AT1G55803.1 70.968 62 18 0 16 77 61 122 2.00e-27 104
Msa1144370 AT1G55800.1 54.762 84 38 0 86 169 11 94 2.94e-26 104
Msa1144370 AT5G25030.1 36.129 155 84 3 16 169 51 191 7.65e-26 102
Msa1144370 AT2G11045.1 34.615 156 65 4 16 169 14 134 1.63e-16 75.5

Find 129 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGCAACTTCGTGAAATTTA+AGG 0.134308 7_4:-20305274 Msa1144370:five_prime_UTR
CACATAGTCTCTCAAGTATT+TGG 0.136523 7_4:+20303236 None:intergenic
ATTTCACGAAGTTGCGATTT+TGG 0.149292 7_4:+20305280 None:intergenic
GTCCTCGACATTGTCATTTA+TGG 0.155281 7_4:-20304873 Msa1144370:five_prime_UTR
ACTATTAAAGCATTATAAAT+AGG 0.172562 7_4:+20305496 None:intergenic
GAAATAATGTTATACTATAA+TGG 0.203719 7_4:-20305384 Msa1144370:five_prime_UTR
CTTTCTATTGCAAAAGCTTT+TGG 0.255346 7_4:-20305574 Msa1144370:five_prime_UTR
ATCAAAAGTTTGAAATAAAA+AGG 0.260393 7_4:+20304777 None:intergenic
GCTTCTTCACTTGACACTTA+TGG 0.273879 7_4:-20305532 Msa1144370:five_prime_UTR
CAATTCTTCTAAATTTGATT+TGG 0.284080 7_4:+20304425 None:intergenic
AAGTCAAATTCTGATCTAAT+TGG 0.285572 7_4:+20303480 None:intergenic
AAATAATGTTATACTATAAT+GGG 0.300940 7_4:-20305383 Msa1144370:five_prime_UTR
GGATCCCTTGTTTGCCTACT+AGG 0.306423 7_4:-20303199 Msa1144370:three_prime_UTR
TGGTTGCTTTGAATGAAAAT+AGG 0.308725 7_4:-20304583 Msa1144370:five_prime_UTR
ATTCCTTTATGAATAAATAA+AGG 0.315842 7_4:+20305232 None:intergenic
TGTTGTATTAGTGTTGGTTT+AGG 0.317949 7_4:-20305073 Msa1144370:five_prime_UTR
TGAAGGAGAAGGGGATGTTA+TGG 0.320084 7_4:-20303367 Msa1144370:CDS
AAGCCTTTATTTATTCATAA+AGG 0.328016 7_4:-20305235 Msa1144370:five_prime_UTR
CTCTGAATCTCTTCATGAAA+TGG 0.328925 7_4:+20303621 None:intergenic
GGTTTAGGGTTTGAAGTTTA+CGG 0.330361 7_4:-20305058 Msa1144370:five_prime_UTR
CGCAACTTCGTGAAATTTAA+GGG 0.343963 7_4:-20305273 Msa1144370:five_prime_UTR
CTGTAGTGCCATTAGATATA+AGG 0.355231 7_4:-20303134 Msa1144370:three_prime_UTR
TCCAGCTCTTCTCCTCCACT+TGG 0.364659 7_4:+20303312 None:intergenic
GTTGTATTAGTGTTGGTTTA+GGG 0.369914 7_4:-20305072 Msa1144370:five_prime_UTR
TCAGAATTTGACTTGAGAAA+TGG 0.370628 7_4:-20303471 Msa1144370:CDS
TAGTAGCAACTTTCTTTGAT+TGG 0.376444 7_4:+20304913 None:intergenic
CTATTAAAGCATTATAAATA+GGG 0.378704 7_4:+20305497 None:intergenic
AGAATGCATGTCAGATGCTT+AGG 0.383246 7_4:-20303926 Msa1144370:CDS
CGAAGATAGTTGAGATCAAC+TGG 0.391483 7_4:+20303579 None:intergenic
CTATATGATTTCCCCTCAAA+TGG 0.396400 7_4:+20303670 None:intergenic
GTGAATTTAAAGGATGCATA+TGG 0.407557 7_4:-20303970 Msa1144370:intron
TTTAGAAGAATTGCAGAAGC+TGG 0.410090 7_4:-20304415 Msa1144370:CDS
CAATCATTGTCAGAAAGCTT+TGG 0.412205 7_4:+20303829 None:intergenic
GGGTCTGGTGCGGTTCCTCT+TGG 0.414919 7_4:+20303257 None:intergenic
CCTCGTTTGTTGTTGTAACC+TGG 0.415076 7_4:+20303777 None:intergenic
ACATAGTCTCTCAAGTATTT+GGG 0.415278 7_4:+20303237 None:intergenic
TTTGTTTGTGTAGACAATTA+TGG 0.418283 7_4:-20304841 Msa1144370:five_prime_UTR
GTCTCTCAAGTATTTGGGTC+TGG 0.419801 7_4:+20303242 None:intergenic
GGTTTGAAGTTTACGGTTGA+AGG 0.420613 7_4:-20305051 Msa1144370:five_prime_UTR
CACTAAGGCCTTATATCTAA+TGG 0.420923 7_4:+20303126 None:intergenic
TCAAGTTGTCTTCCTTCCTA+TGG 0.423108 7_4:+20304286 None:intergenic
CAAAGAAAGTTGCTACTACC+AGG 0.427859 7_4:-20304908 Msa1144370:five_prime_UTR
GCGGTTCCTCTTGGCCCGTA+TGG 0.428241 7_4:+20303266 None:intergenic
TGCTACAGTTGCAAAACTTA+GGG 0.429481 7_4:-20305334 Msa1144370:five_prime_UTR
TCGTTGTTTCTTTGAAATGA+AGG 0.434958 7_4:-20305196 Msa1144370:five_prime_UTR
CAAAATTGACCTTGTCCTCA+AGG 0.439623 7_4:+20303388 None:intergenic
CGGTTCCTCTTGGCCCGTAT+GGG 0.439724 7_4:+20303267 None:intergenic
CCCAAGTGGAGGAGAAGAGC+TGG 0.446261 7_4:-20303313 Msa1144370:CDS
ACCACCTCATCTACATGAAC+AGG 0.446479 7_4:+20304465 None:intergenic
CTGCTACAGTTGCAAAACTT+AGG 0.451776 7_4:-20305335 Msa1144370:five_prime_UTR
CTGCACTTACCGAGAGGAAA+GGG 0.452267 7_4:+20303732 None:intergenic
AACAGTAATGAAATTTCATC+CGG 0.452991 7_4:-20304364 Msa1144370:CDS
CCATTCCTAGTAGGCAAACA+AGG 0.464092 7_4:+20303194 None:intergenic
TTCAACAAGGAAGATTATCC+AGG 0.465047 7_4:-20303795 Msa1144370:CDS
ATGCATATGGATCTCGTGTT+CGG 0.467055 7_4:-20303957 Msa1144370:CDS
ACTTGGGTCAGCAAGTTCAA+TGG 0.467706 7_4:+20303329 None:intergenic
CTTGGGTCAGCAAGTTCAAT+GGG 0.471454 7_4:+20303330 None:intergenic
CTACACCTCCTTTCAATGAC+TGG 0.471883 7_4:-20303872 Msa1144370:CDS
CCTTGTTTGCCTACTAGGAA+TGG 0.473495 7_4:-20303194 Msa1144370:three_prime_UTR
GTTTAAGTAGTGTGTCTCAA+TGG 0.482186 7_4:-20304686 Msa1144370:five_prime_UTR
ATATCTAATGGCACTACAGC+TGG 0.488920 7_4:+20303138 None:intergenic
ATATTCAACTTTCCTCATGC+AGG 0.489206 7_4:-20304312 Msa1144370:CDS
CATTGTCAGAAAGCTTTGGT+TGG 0.492728 7_4:+20303833 None:intergenic
TGATCATCAAATACTGTTAG+TGG 0.493099 7_4:-20305620 Msa1144370:five_prime_UTR
GTAGTGTGTCTCAATGGACT+TGG 0.495427 7_4:-20304680 Msa1144370:five_prime_UTR
TGTTGATGTTGTATTAGTGT+TGG 0.496792 7_4:-20305079 Msa1144370:five_prime_UTR
CTGGGAGCATATGTGTTACA+TGG 0.500115 7_4:-20304396 Msa1144370:CDS
TTGTTGATCTTCCTTGAGAT+CGG 0.500723 7_4:+20303441 None:intergenic
AGACCTTATCCATTCCTAGT+AGG 0.500939 7_4:+20303185 None:intergenic
CATTAGATATAAGGCCTTAG+TGG 0.506734 7_4:-20303125 Msa1144370:three_prime_UTR
TCTGCAAAATCAAAATGCTC+TGG 0.507034 7_4:-20304512 Msa1144370:exon
AGTGCATGTAAGAAGAAACT+AGG 0.508831 7_4:-20305691 Msa1144370:five_prime_UTR
TTGCCTACTAGGAATGGATA+AGG 0.510045 7_4:-20303188 Msa1144370:three_prime_UTR
AGGAATGGATAAGGTCTTAG+TGG 0.511022 7_4:-20303179 Msa1144370:three_prime_UTR
AAACTATACCATATTAGATA+AGG 0.512810 7_4:+20304616 None:intergenic
TAGTTGAGATCAACTGGAGC+TGG 0.515891 7_4:+20303585 None:intergenic
GAATGCATGTCAGATGCTTA+GGG 0.519691 7_4:-20303925 Msa1144370:CDS
TTAGAAGAATTGCAGAAGCT+GGG 0.525297 7_4:-20304414 Msa1144370:CDS
TATAATATAATGTTCCACTA+AGG 0.528049 7_4:+20303111 None:intergenic
CCAGCTCTTCTCCTCCACTT+GGG 0.530467 7_4:+20303313 None:intergenic
GAGAAGAGCTGGAAGAAGAC+AGG 0.532441 7_4:-20303302 Msa1144370:CDS
AGTAATGTGGCAAAATTTGA+CGG 0.537095 7_4:+20304534 None:intergenic
TTCTTTGAAATGAAGGTGCA+TGG 0.541482 7_4:-20305189 Msa1144370:five_prime_UTR
TCCTGTTCATGTAGATGAGG+TGG 0.543865 7_4:-20304466 Msa1144370:CDS
CAAATACTGTTAGTGGGTGA+TGG 0.554164 7_4:-20305613 Msa1144370:five_prime_UTR
TGTGATGACCCCTTTCCTCT+CGG 0.554223 7_4:-20303741 Msa1144370:CDS
ATGGATACAATCCGATCTCA+AGG 0.558734 7_4:-20303452 Msa1144370:CDS
GGATCCATGTTACTCCCATA+CGG 0.560176 7_4:-20303281 Msa1144370:CDS
CAAATACTTGAGAGACTATG+TGG 0.561291 7_4:-20303235 Msa1144370:CDS
TCAATGTTCCAGTCATTGAA+AGG 0.564225 7_4:+20303864 None:intergenic
GATCATCAAATACTGTTAGT+GGG 0.567279 7_4:-20305619 Msa1144370:five_prime_UTR
CTATGTGGATCAGTAAGCAC+AGG 0.573416 7_4:-20303220 Msa1144370:exon
TCATGCAGGTTTCCATAGGA+AGG 0.577731 7_4:-20304298 Msa1144370:CDS
TTTAGAAAGTGATTTGTCTG+AGG 0.579433 7_4:+20303513 None:intergenic
ACTTCTGAACCTTGAGGACA+AGG 0.580193 7_4:-20303397 Msa1144370:CDS
CCCTCCTGTTCATGTAGATG+AGG 0.581123 7_4:-20304469 Msa1144370:CDS
GCTGCACTTACCGAGAGGAA+AGG 0.583209 7_4:+20303731 None:intergenic
TCAAGTATTTGGGTCTGGTG+CGG 0.585653 7_4:+20303247 None:intergenic
TTCCTCATGCAGGTTTCCAT+AGG 0.587250 7_4:-20304302 Msa1144370:CDS
TGACAATGTCGAGGACTCGA+GGG 0.588556 7_4:+20304880 None:intergenic
AGCATATGTGTTACATGGAG+TGG 0.589305 7_4:-20304391 Msa1144370:CDS
ATGACAATGTCGAGGACTCG+AGG 0.589338 7_4:+20304879 None:intergenic
GTGCATGTAAGAAGAAACTA+GGG 0.590755 7_4:-20305690 Msa1144370:five_prime_UTR
TGTCAGATGCTTAGGGCCAA+TGG 0.593917 7_4:-20303918 Msa1144370:CDS
AAAGAAAGTTGCTACTACCA+GGG 0.597849 7_4:-20304907 Msa1144370:five_prime_UTR
TCTAATGGCACTACAGCTGG+TGG 0.609934 7_4:+20303141 None:intergenic
GATCCATGTTACTCCCATAC+GGG 0.611469 7_4:-20303280 Msa1144370:CDS
TGAAATACTTCTGAACCTTG+AGG 0.612747 7_4:-20303403 Msa1144370:CDS
ACCTCATCTACATGAACAGG+AGG 0.612752 7_4:+20304468 None:intergenic
GCAACTTCGTGAAATTTAAG+GGG 0.613979 7_4:-20305272 Msa1144370:five_prime_UTR
ATTGACATGAATCTCACCAT+TGG 0.626240 7_4:+20303902 None:intergenic
TGGCCCGTATGGGAGTAACA+TGG 0.633048 7_4:+20303277 None:intergenic
GCTACAGTTGCAAAACTTAG+GGG 0.634426 7_4:-20305333 Msa1144370:five_prime_UTR
TTGTGTTCAATCTGAAGTGT+CGG 0.635703 7_4:-20305015 Msa1144370:five_prime_UTR
CATTCCTAGTAGGCAAACAA+GGG 0.636597 7_4:+20303195 None:intergenic
AACCATAAATGACAATGTCG+AGG 0.641431 7_4:+20304871 None:intergenic
GAACTTGCTGACCCAAGTGG+AGG 0.643038 7_4:-20303324 Msa1144370:CDS
ATTGAACTTGCTGACCCAAG+TGG 0.657087 7_4:-20303327 Msa1144370:CDS
CTACAGTTGCAAAACTTAGG+GGG 0.657466 7_4:-20305332 Msa1144370:five_prime_UTR
TGCACTTACCGAGAGGAAAG+GGG 0.664703 7_4:+20303733 None:intergenic
TTCCTATGGAAACCTGCATG+AGG 0.665648 7_4:+20304300 None:intergenic
CCAGGTTACAACAACAAACG+AGG 0.667964 7_4:-20303777 Msa1144370:CDS
ATGTTCCAGTCATTGAAAGG+AGG 0.678903 7_4:+20303867 None:intergenic
TTACTCCCATACGGGCCAAG+AGG 0.679246 7_4:-20303272 Msa1144370:CDS
TGACTGCATTGATTTCAACA+AGG 0.696255 7_4:-20303808 Msa1144370:CDS
GTGCATGTGAATGTAATGCA+TGG 0.711013 7_4:-20304725 Msa1144370:five_prime_UTR
GAGGACTCGAGGGTTAACCC+TGG 0.717253 7_4:+20304890 None:intergenic
CCTCATCTACATGAACAGGA+GGG 0.718871 7_4:+20304469 None:intergenic
AAAGTGCTGCACTTACCGAG+AGG 0.719258 7_4:+20303726 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATTTTGATATTTGAAT+TGG - chr7_4:20303287-20303306 Msa1144370:CDS 10.0%
!! AAATAATGTTATACTATAAT+GGG - chr7_4:20303330-20303349 Msa1144370:CDS 10.0%
!!! TAATTAACATATTTCTAAAT+AGG + chr7_4:20304651-20304670 None:intergenic 10.0%
!!! CTATTAAAGCATTATAAATA+GGG + chr7_4:20303219-20303238 None:intergenic 15.0%
!!! ACTATTAAAGCATTATAAAT+AGG + chr7_4:20303220-20303239 None:intergenic 15.0%
!! GAAATAATGTTATACTATAA+TGG - chr7_4:20303329-20303348 Msa1144370:CDS 15.0%
!!! TTTTGTAGTTTTTTTTTCTT+AGG - chr7_4:20303407-20303426 Msa1144370:CDS 15.0%
!!! TTTGTAGTTTTTTTTTCTTA+GGG - chr7_4:20303408-20303427 Msa1144370:CDS 15.0%
!! ATTCCTTTATGAATAAATAA+AGG + chr7_4:20303484-20303503 None:intergenic 15.0%
!!! ACATAGTTATTTTTTTTGAT+CGG - chr7_4:20303725-20303744 Msa1144370:CDS 15.0%
!!! ATCAAAAGTTTGAAATAAAA+AGG + chr7_4:20303939-20303958 None:intergenic 15.0%
!! AGAAATATGTTAATTATCTT+TGG - chr7_4:20304655-20304674 Msa1144370:five_prime_UTR 15.0%
!!! ATTTCTTTAAGAAAAAGAAA+AGG - chr7_4:20305707-20305726 Msa1144370:five_prime_UTR 15.0%
!! TTTAACACTAAACGAAAAAA+GGG + chr7_4:20303271-20303290 None:intergenic 20.0%
!!! TTTTAACACTAAACGAAAAA+AGG + chr7_4:20303272-20303291 None:intergenic 20.0%
!!! TTGTAGTTTTTTTTTCTTAG+GGG - chr7_4:20303409-20303428 Msa1144370:CDS 20.0%
!!! AAGCCTTTATTTATTCATAA+AGG - chr7_4:20303478-20303497 Msa1144370:CDS 20.0%
!!! GTGTTTTTGAATATGATAAA+AGG - chr7_4:20303747-20303766 Msa1144370:CDS 20.0%
!!! GTTTTTTTCCTTATCTAATA+TGG - chr7_4:20304089-20304108 Msa1144370:intron 20.0%
!! AAACTATACCATATTAGATA+AGG + chr7_4:20304100-20304119 None:intergenic 20.0%
!! CAATTCTTCTAAATTTGATT+TGG + chr7_4:20304291-20304310 None:intergenic 20.0%
!! ACTTGTGCATAATAATAAAA+GGG + chr7_4:20304607-20304626 None:intergenic 20.0%
!! AACTTGTGCATAATAATAAA+AGG + chr7_4:20304608-20304627 None:intergenic 20.0%
!!! CAATTGAATTGATTCATTTT+TGG + chr7_4:20305176-20305195 None:intergenic 20.0%
!! TATAATATAATGTTCCACTA+AGG + chr7_4:20305605-20305624 None:intergenic 20.0%
!!! TGTAGTTTTTTTTTCTTAGG+GGG - chr7_4:20303410-20303429 Msa1144370:CDS 25.0%
!!! ATGAGTATCATTTTTATAGC+TGG + chr7_4:20303555-20303574 None:intergenic 25.0%
! TTTGTTTGTGTAGACAATTA+TGG - chr7_4:20303872-20303891 Msa1144370:CDS 25.0%
!! TTCCTATTGTTCACAATTTT+TGG - chr7_4:20303963-20303982 Msa1144370:intron 25.0%
!! TCCTATTGTTCACAATTTTT+GGG - chr7_4:20303964-20303983 Msa1144370:intron 25.0%
!!! CTAATATGGTATAGTTTTGA+AGG - chr7_4:20304103-20304122 Msa1144370:intron 25.0%
! AACAGTAATGAAATTTCATC+CGG - chr7_4:20304349-20304368 Msa1144370:CDS 25.0%
!! TTGAGTACTTTTAAACACTT+CGG - chr7_4:20304506-20304525 Msa1144370:exon 25.0%
! CTTGTGCATAATAATAAAAG+GGG + chr7_4:20304606-20304625 None:intergenic 25.0%
! ATCTTTGGTTATCATAGTTA+AGG - chr7_4:20304670-20304689 Msa1144370:five_prime_UTR 25.0%
!! AAATGGTTTTTCATACTTCT+AGG + chr7_4:20305029-20305048 None:intergenic 25.0%
! AAGTATGAAAAACCATTTGA+GGG - chr7_4:20305031-20305050 Msa1144370:five_prime_UTR 25.0%
! AAGTCAAATTCTGATCTAAT+TGG + chr7_4:20305236-20305255 None:intergenic 25.0%
!! CTTTTCTTCTTTGATTTTCG+TGG + chr7_4:20303004-20303023 None:intergenic 30.0%
AGCAACAACAACAACAAAAA+TGG - chr7_4:20303053-20303072 Msa1144370:three_prime_UTR 30.0%
GCAACAACAACAACAAAAAT+GGG - chr7_4:20303054-20303073 Msa1144370:three_prime_UTR 30.0%
TGATCATCAAATACTGTTAG+TGG - chr7_4:20303093-20303112 Msa1144370:three_prime_UTR 30.0%
GATCATCAAATACTGTTAGT+GGG - chr7_4:20303094-20303113 Msa1144370:three_prime_UTR 30.0%
! CTTTCTATTGCAAAAGCTTT+TGG - chr7_4:20303139-20303158 Msa1144370:three_prime_UTR 30.0%
!!! TTGGATGCATGTTTTTTAGT+TGG - chr7_4:20303306-20303325 Msa1144370:CDS 30.0%
!!! TGGATGCATGTTTTTTAGTT+GGG - chr7_4:20303307-20303326 Msa1144370:CDS 30.0%
!! TCGTTGTTTCTTTGAAATGA+AGG - chr7_4:20303517-20303536 Msa1144370:CDS 30.0%
TGTTGATGTTGTATTAGTGT+TGG - chr7_4:20303634-20303653 Msa1144370:CDS 30.0%
!! TGTTGTATTAGTGTTGGTTT+AGG - chr7_4:20303640-20303659 Msa1144370:CDS 30.0%
!! GTTGTATTAGTGTTGGTTTA+GGG - chr7_4:20303641-20303660 Msa1144370:CDS 30.0%
TAGTAGCAACTTTCTTTGAT+TGG + chr7_4:20303803-20303822 None:intergenic 30.0%
TCCCAAAAATTGTGAACAAT+AGG + chr7_4:20303968-20303987 None:intergenic 30.0%
! TGGTTGCTTTGAATGAAAAT+AGG - chr7_4:20304130-20304149 Msa1144370:intron 30.0%
AGTAATGTGGCAAAATTTGA+CGG + chr7_4:20304182-20304201 None:intergenic 30.0%
!! TTTGATTTTGCAGAGTAATG+TGG + chr7_4:20304195-20304214 None:intergenic 30.0%
! AACCTCTGCATTTTTAAATC+CGG + chr7_4:20304371-20304390 None:intergenic 30.0%
GTGTTTCCTTGTGAATTTAA+AGG - chr7_4:20304733-20304752 Msa1144370:five_prime_UTR 30.0%
ATGCATCCTTTAAATTCACA+AGG + chr7_4:20304742-20304761 None:intergenic 30.0%
GTGAATTTAAAGGATGCATA+TGG - chr7_4:20304743-20304762 Msa1144370:five_prime_UTR 30.0%
GAAGTATGAAAAACCATTTG+AGG - chr7_4:20305030-20305049 Msa1144370:five_prime_UTR 30.0%
AGTATGAAAAACCATTTGAG+GGG - chr7_4:20305032-20305051 Msa1144370:five_prime_UTR 30.0%
! TTTAGAAAGTGATTTGTCTG+AGG + chr7_4:20305203-20305222 None:intergenic 30.0%
!! TCAGAATTTGACTTGAGAAA+TGG - chr7_4:20305242-20305261 Msa1144370:five_prime_UTR 30.0%
ACATAGTCTCTCAAGTATTT+GGG + chr7_4:20305479-20305498 None:intergenic 30.0%
AGTGCATGTAAGAAGAAACT+AGG - chr7_4:20303022-20303041 Msa1144370:three_prime_UTR 35.0%
GTGCATGTAAGAAGAAACTA+GGG - chr7_4:20303023-20303042 Msa1144370:three_prime_UTR 35.0%
TGCTACAGTTGCAAAACTTA+GGG - chr7_4:20303379-20303398 Msa1144370:CDS 35.0%
! ATTTCACGAAGTTGCGATTT+TGG + chr7_4:20303436-20303455 None:intergenic 35.0%
TCGCAACTTCGTGAAATTTA+AGG - chr7_4:20303439-20303458 Msa1144370:CDS 35.0%
CGCAACTTCGTGAAATTTAA+GGG - chr7_4:20303440-20303459 Msa1144370:CDS 35.0%
GCAACTTCGTGAAATTTAAG+GGG - chr7_4:20303441-20303460 Msa1144370:CDS 35.0%
!! TTCTTTGAAATGAAGGTGCA+TGG - chr7_4:20303524-20303543 Msa1144370:CDS 35.0%
! GGTTTAGGGTTTGAAGTTTA+CGG - chr7_4:20303655-20303674 Msa1144370:CDS 35.0%
! TTGTGTTCAATCTGAAGTGT+CGG - chr7_4:20303698-20303717 Msa1144370:CDS 35.0%
AAAGAAAGTTGCTACTACCA+GGG - chr7_4:20303806-20303825 Msa1144370:CDS 35.0%
AACCATAAATGACAATGTCG+AGG + chr7_4:20303845-20303864 None:intergenic 35.0%
!!! GCATGGTGATGTATTTTGAT+TGG - chr7_4:20304005-20304024 Msa1144370:intron 35.0%
GTTTAAGTAGTGTGTCTCAA+TGG - chr7_4:20304027-20304046 Msa1144370:intron 35.0%
TCTGCAAAATCAAAATGCTC+TGG - chr7_4:20304201-20304220 Msa1144370:intron 35.0%
TTTAGAAGAATTGCAGAAGC+TGG - chr7_4:20304298-20304317 Msa1144370:CDS 35.0%
TTAGAAGAATTGCAGAAGCT+GGG - chr7_4:20304299-20304318 Msa1144370:CDS 35.0%
ATCCGGATTTAAAAATGCAG+AGG - chr7_4:20304366-20304385 Msa1144370:CDS 35.0%
ATATTCAACTTTCCTCATGC+AGG - chr7_4:20304401-20304420 Msa1144370:CDS 35.0%
! TACTTTTAAACACTTCGGAG+TGG - chr7_4:20304511-20304530 Msa1144370:exon 35.0%
ATTGACATGAATCTCACCAT+TGG + chr7_4:20304814-20304833 None:intergenic 35.0%
TCAATGTTCCAGTCATTGAA+AGG + chr7_4:20304852-20304871 None:intergenic 35.0%
CAATCATTGTCAGAAAGCTT+TGG + chr7_4:20304887-20304906 None:intergenic 35.0%
!! TGACTGCATTGATTTCAACA+AGG - chr7_4:20304905-20304924 Msa1144370:five_prime_UTR 35.0%
TTCAACAAGGAAGATTATCC+AGG - chr7_4:20304918-20304937 Msa1144370:five_prime_UTR 35.0%
CTATATGATTTCCCCTCAAA+TGG + chr7_4:20305046-20305065 None:intergenic 35.0%
CTCTGAATCTCTTCATGAAA+TGG + chr7_4:20305095-20305114 None:intergenic 35.0%
TTGTTGATCTTCCTTGAGAT+CGG + chr7_4:20305275-20305294 None:intergenic 35.0%
TGAAATACTTCTGAACCTTG+AGG - chr7_4:20305310-20305329 Msa1144370:five_prime_UTR 35.0%
!! AAGGTCAATTTTGAAGGAGA+AGG - chr7_4:20305335-20305354 Msa1144370:five_prime_UTR 35.0%
!! AGGTCAATTTTGAAGGAGAA+GGG - chr7_4:20305336-20305355 Msa1144370:five_prime_UTR 35.0%
CACATAGTCTCTCAAGTATT+TGG + chr7_4:20305480-20305499 None:intergenic 35.0%
CAAATACTTGAGAGACTATG+TGG - chr7_4:20305478-20305497 Msa1144370:five_prime_UTR 35.0%
! CTGTAGTGCCATTAGATATA+AGG - chr7_4:20305579-20305598 Msa1144370:five_prime_UTR 35.0%
! CACTAAGGCCTTATATCTAA+TGG + chr7_4:20305590-20305609 None:intergenic 35.0%
CATTAGATATAAGGCCTTAG+TGG - chr7_4:20305588-20305607 Msa1144370:five_prime_UTR 35.0%
! CAAATACTGTTAGTGGGTGA+TGG - chr7_4:20303100-20303119 Msa1144370:three_prime_UTR 40.0%
GCTTCTTCACTTGACACTTA+TGG - chr7_4:20303181-20303200 Msa1144370:three_prime_UTR 40.0%
CTGCTACAGTTGCAAAACTT+AGG - chr7_4:20303378-20303397 Msa1144370:CDS 40.0%
GCTACAGTTGCAAAACTTAG+GGG - chr7_4:20303380-20303399 Msa1144370:CDS 40.0%
CTACAGTTGCAAAACTTAGG+GGG - chr7_4:20303381-20303400 Msa1144370:CDS 40.0%
! GGTTTGAAGTTTACGGTTGA+AGG - chr7_4:20303662-20303681 Msa1144370:CDS 40.0%
CAAAGAAAGTTGCTACTACC+AGG - chr7_4:20303805-20303824 Msa1144370:CDS 40.0%
! GTCCTCGACATTGTCATTTA+TGG - chr7_4:20303840-20303859 Msa1144370:CDS 40.0%
GTGCATGTGAATGTAATGCA+TGG - chr7_4:20303988-20304007 Msa1144370:intron 40.0%
!!! GGTATAGTTTTGAAGGTGCT+TGG - chr7_4:20304110-20304129 Msa1144370:intron 40.0%
AGCATATGTGTTACATGGAG+TGG - chr7_4:20304322-20304341 Msa1144370:CDS 40.0%
! TCAAGTTGTCTTCCTTCCTA+TGG + chr7_4:20304430-20304449 None:intergenic 40.0%
ATGCATATGGATCTCGTGTT+CGG - chr7_4:20304756-20304775 Msa1144370:five_prime_UTR 40.0%
AGAATGCATGTCAGATGCTT+AGG - chr7_4:20304787-20304806 Msa1144370:five_prime_UTR 40.0%
GAATGCATGTCAGATGCTTA+GGG - chr7_4:20304788-20304807 Msa1144370:five_prime_UTR 40.0%
ATGTTCCAGTCATTGAAAGG+AGG + chr7_4:20304849-20304868 None:intergenic 40.0%
! CATTGTCAGAAAGCTTTGGT+TGG + chr7_4:20304883-20304902 None:intergenic 40.0%
CGAAGATAGTTGAGATCAAC+TGG + chr7_4:20305137-20305156 None:intergenic 40.0%
ATGGATACAATCCGATCTCA+AGG - chr7_4:20305261-20305280 Msa1144370:five_prime_UTR 40.0%
CAAAATTGACCTTGTCCTCA+AGG + chr7_4:20305328-20305347 None:intergenic 40.0%
! GAGGACAAGGTCAATTTTGA+AGG - chr7_4:20305329-20305348 Msa1144370:five_prime_UTR 40.0%
!! GGTCAATTTTGAAGGAGAAG+GGG - chr7_4:20305337-20305356 Msa1144370:five_prime_UTR 40.0%
CATTCCTAGTAGGCAAACAA+GGG + chr7_4:20305521-20305540 None:intergenic 40.0%
TTGCCTACTAGGAATGGATA+AGG - chr7_4:20305525-20305544 Msa1144370:five_prime_UTR 40.0%
AGACCTTATCCATTCCTAGT+AGG + chr7_4:20305531-20305550 None:intergenic 40.0%
AGGAATGGATAAGGTCTTAG+TGG - chr7_4:20305534-20305553 Msa1144370:five_prime_UTR 40.0%
ATATCTAATGGCACTACAGC+TGG + chr7_4:20305578-20305597 None:intergenic 40.0%
! GTAGTGTGTCTCAATGGACT+TGG - chr7_4:20304033-20304052 Msa1144370:intron 45.0%
CCTCATCTACATGAACAGGA+GGG + chr7_4:20304247-20304266 None:intergenic 45.0%
ACCTCATCTACATGAACAGG+AGG + chr7_4:20304248-20304267 None:intergenic 45.0%
TCCTGTTCATGTAGATGAGG+TGG - chr7_4:20304247-20304266 Msa1144370:intron 45.0%
ACCACCTCATCTACATGAAC+AGG + chr7_4:20304251-20304270 None:intergenic 45.0%
! CTGGGAGCATATGTGTTACA+TGG - chr7_4:20304317-20304336 Msa1144370:CDS 45.0%
TTCCTCATGCAGGTTTCCAT+AGG - chr7_4:20304411-20304430 Msa1144370:CDS 45.0%
TTCCTATGGAAACCTGCATG+AGG + chr7_4:20304416-20304435 None:intergenic 45.0%
TCATGCAGGTTTCCATAGGA+AGG - chr7_4:20304415-20304434 Msa1144370:CDS 45.0%
CTACACCTCCTTTCAATGAC+TGG - chr7_4:20304841-20304860 Msa1144370:five_prime_UTR 45.0%
!! CCTCGTTTGTTGTTGTAACC+TGG + chr7_4:20304939-20304958 None:intergenic 45.0%
CCAGGTTACAACAACAAACG+AGG - chr7_4:20304936-20304955 Msa1144370:five_prime_UTR 45.0%
TAGTTGAGATCAACTGGAGC+TGG + chr7_4:20305131-20305150 None:intergenic 45.0%
ACTTCTGAACCTTGAGGACA+AGG - chr7_4:20305316-20305335 Msa1144370:five_prime_UTR 45.0%
TGAAGGAGAAGGGGATGTTA+TGG - chr7_4:20305346-20305365 Msa1144370:five_prime_UTR 45.0%
CTTGGGTCAGCAAGTTCAAT+GGG + chr7_4:20305386-20305405 None:intergenic 45.0%
ACTTGGGTCAGCAAGTTCAA+TGG + chr7_4:20305387-20305406 None:intergenic 45.0%
!! ATTGAACTTGCTGACCCAAG+TGG - chr7_4:20305386-20305405 Msa1144370:five_prime_UTR 45.0%
GGATCCATGTTACTCCCATA+CGG - chr7_4:20305432-20305451 Msa1144370:five_prime_UTR 45.0%
GATCCATGTTACTCCCATAC+GGG - chr7_4:20305433-20305452 Msa1144370:five_prime_UTR 45.0%
TCAAGTATTTGGGTCTGGTG+CGG + chr7_4:20305469-20305488 None:intergenic 45.0%
GTCTCTCAAGTATTTGGGTC+TGG + chr7_4:20305474-20305493 None:intergenic 45.0%
CTATGTGGATCAGTAAGCAC+AGG - chr7_4:20305493-20305512 Msa1144370:five_prime_UTR 45.0%
CCATTCCTAGTAGGCAAACA+AGG + chr7_4:20305522-20305541 None:intergenic 45.0%
! CCTTGTTTGCCTACTAGGAA+TGG - chr7_4:20305519-20305538 Msa1144370:five_prime_UTR 45.0%
CAGTTGCAAAACTTAGGGGG+CGG - chr7_4:20303384-20303403 Msa1144370:CDS 50.0%
TGACAATGTCGAGGACTCGA+GGG + chr7_4:20303836-20303855 None:intergenic 50.0%
ATGACAATGTCGAGGACTCG+AGG + chr7_4:20303837-20303856 None:intergenic 50.0%
CCCTCCTGTTCATGTAGATG+AGG - chr7_4:20304244-20304263 Msa1144370:intron 50.0%
TGTCAGATGCTTAGGGCCAA+TGG - chr7_4:20304795-20304814 Msa1144370:five_prime_UTR 50.0%
TGTGATGACCCCTTTCCTCT+CGG - chr7_4:20304972-20304991 Msa1144370:five_prime_UTR 50.0%
TGCACTTACCGAGAGGAAAG+GGG + chr7_4:20304983-20305002 None:intergenic 50.0%
CTGCACTTACCGAGAGGAAA+GGG + chr7_4:20304984-20305003 None:intergenic 50.0%
!! AAAGTGCTGCACTTACCGAG+AGG + chr7_4:20304990-20305009 None:intergenic 50.0%
GAGAAGAGCTGGAAGAAGAC+AGG - chr7_4:20305411-20305430 Msa1144370:five_prime_UTR 50.0%
! GGATCCCTTGTTTGCCTACT+AGG - chr7_4:20305514-20305533 Msa1144370:five_prime_UTR 50.0%
TCTAATGGCACTACAGCTGG+TGG + chr7_4:20305575-20305594 None:intergenic 50.0%
GCTGCACTTACCGAGAGGAA+AGG + chr7_4:20304985-20305004 None:intergenic 55.0%
!! GAACTTGCTGACCCAAGTGG+AGG - chr7_4:20305389-20305408 Msa1144370:five_prime_UTR 55.0%
CCAGCTCTTCTCCTCCACTT+GGG + chr7_4:20305403-20305422 None:intergenic 55.0%
TCCAGCTCTTCTCCTCCACT+TGG + chr7_4:20305404-20305423 None:intergenic 55.0%
TGGCCCGTATGGGAGTAACA+TGG + chr7_4:20305439-20305458 None:intergenic 55.0%
TTACTCCCATACGGGCCAAG+AGG - chr7_4:20305441-20305460 Msa1144370:five_prime_UTR 55.0%
GAGGACTCGAGGGTTAACCC+TGG + chr7_4:20303826-20303845 None:intergenic 60.0%
CCCAAGTGGAGGAGAAGAGC+TGG - chr7_4:20305400-20305419 Msa1144370:five_prime_UTR 60.0%
CGGTTCCTCTTGGCCCGTAT+GGG + chr7_4:20305449-20305468 None:intergenic 60.0%
GCGGTTCCTCTTGGCCCGTA+TGG + chr7_4:20305450-20305469 None:intergenic 65.0%
GGGTCTGGTGCGGTTCCTCT+TGG + chr7_4:20305459-20305478 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 20303000 20305735 20303000 ID=Msa1144370;Name=Msa1144370
chr7_4 mRNA 20303000 20305735 20303000 ID=Msa1144370-mRNA-1;Parent=Msa1144370;Name=Msa1144370-mRNA-1;_AED=0.08;_eAED=0.08;_QI=1215|1|1|1|1|1|2|227|332
chr7_4 exon 20303000 20303980 20303000 ID=Msa1144370-mRNA-1:exon:4888;Parent=Msa1144370-mRNA-1
chr7_4 exon 20304276 20305735 20304276 ID=Msa1144370-mRNA-1:exon:4887;Parent=Msa1144370-mRNA-1
chr7_4 five_prime_UTR 20304521 20305735 20304521 ID=Msa1144370-mRNA-1:five_prime_utr;Parent=Msa1144370-mRNA-1
chr7_4 CDS 20304276 20304520 20304276 ID=Msa1144370-mRNA-1:cds;Parent=Msa1144370-mRNA-1
chr7_4 CDS 20303227 20303980 20303227 ID=Msa1144370-mRNA-1:cds;Parent=Msa1144370-mRNA-1
chr7_4 three_prime_UTR 20303000 20303226 20303000 ID=Msa1144370-mRNA-1:three_prime_utr;Parent=Msa1144370-mRNA-1
Gene Sequence

>Msa1144370

ATGCTCTGGTCTTGCTTTTGTCATCGATGCCCTCCTGTTCATGTAGATGAGGTGGTCAAGAAGTACAAAAATGCCAAATCAAATTTAGAAGAATTGCAGAAGCTGGGAGCATATGTGTTACATGGAGTGGATGCAACAGTAATGAAATTTCATCCGGATTTAAAAATGCAGAGGTTTGATCGCATTATATTCAACTTTCCTCATGCAGGTTTCCATAGGAAGGAAGACAACTTGATGATGATCATGATGCATATGGATCTCGTGTTCGGTTTCTTCAAGAATGCATGTCAGATGCTTAGGGCCAATGGTGAGATTCATGTCAATCACAAAACTACACCTCCTTTCAATGACTGGAACATTGAGAAGCTCGCCAACCAAAGCTTTCTGACAATGATTGACTGCATTGATTTCAACAAGGAAGATTATCCAGGTTACAACAACAAACGAGGAAATAGCTATCGATGTGATGACCCCTTTCCTCTCGGTAAGTGCAGCACTTTCAAGTTCATATGCAATCCTAGAAGTATGAAAAACCATTTGAGGGGAAATCATATAGAGATTTCTAGACAGCAGACAATTCTTCCATTTCATGAAGAGATTCAGAGCATTGCGCAATTTCCAGCTCCAGTTGATCTCAACTATCTTCGTCGAACAAGTTTATTTCCAAAAATGAATCAATTCAATTGTTATCCTCAGACAAATCACTTTCTAAAGTTGAATGAACCAATTAGATCAGAATTTGACTTGAGAAATGGATACAATCCGATCTCAAGGAAGATCAACAATTTGACAGAAATACTTGAAATACTTCTGAACCTTGAGGACAAGGTCAATTTTGAAGGAGAAGGGGATGTTATGGATCACAACATTGAACCCATTGAACTTGCTGACCCAAGTGGAGGAGAAGAGCTGGAAGAAGACAGGATCCATGTTACTCCCATACGGGCCAAGAGGAACCGCACCAGACCCAAATACTTGAGAGACTATGTGGATCAGTAA
Protein sequence

>Msa1144370

MLWSCFCHRCPPVHVDEVVKKYKNAKSNLEELQKLGAYVLHGVDATVMKFHPDLKMQRFDRIIFNFPHAGFHRKEDNLMMIMMHMDLVFGFFKNACQMLRANGEIHVNHKTTPPFNDWNIEKLANQSFLTMIDCIDFNKEDYPGYNNKRGNSYRCDDPFPLGKCSTFKFICNPRSMKNHLRGNHIEISRQQTILPFHEEIQSIAQFPAPVDLNYLRRTSLFPKMNQFNCYPQTNHFLKLNEPIRSEFDLRNGYNPISRKINNLTEILEILLNLEDKVNFEGEGDVMDHNIEPIELADPSGGEELEEDRIHVTPIRAKRNRTRPKYLRDYVDQ*