AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0491920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0491920 MtrunA17_Chr3g0084111 90.580 138 8 1 36 173 1 133 2.61e-89 257
Msa0491920 MtrunA17_Chr8g0360271 35.294 170 68 5 40 173 132 295 3.14e-23 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 262 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTGTGAAAAGTTTA+TGG 0.140829 3_4:+14901437 Msa0491920:CDS
CATTATTACACTTGGTTACT+AGG 0.200427 3_4:-14901572 None:intergenic
CTTGCTCGCGCTGTGAGATT+TGG 0.203037 3_4:-14899844 Msa0491910:CDS
TTGCTCGCGCTGTGAGATTT+GGG 0.207744 3_4:-14899843 Msa0491910:CDS
CTTCCAAATCATGCAATTAC+AGG 0.262290 3_4:-14901772 None:intergenic
CAAGCAAGAGTGAAGTTCTA+TGG 0.303827 3_4:+14901858 Msa0491920:three_prime_UTR
AACCCTGTAATTGCATGATT+TGG 0.330851 3_4:+14901769 Msa0491920:three_prime_UTR
GGAGAAAGGGGTGGCTAGTT+AGG 0.364642 3_4:+14901502 Msa0491920:CDS
TGCATGATTTGGAAGTAAAC+TGG 0.382009 3_4:+14901780 Msa0491920:three_prime_UTR
CTGCAATTCAAAGTATCTAT+AGG 0.418695 3_4:-14901538 None:intergenic
AAAAGTTTATGGATCCTCAT+TGG 0.433326 3_4:+14901448 Msa0491920:CDS
CAAGAATCAACACAAATAGA+TGG 0.434051 3_4:+14901168 Msa0491920:CDS
GGGGTGGCTAGTTAGGCTTT+AGG 0.434842 3_4:+14901509 Msa0491920:CDS
ACACTCAGTAATTGAAGAAC+TGG 0.444867 3_4:-14901367 None:intergenic
CAAGGATGAGTAACAACAAC+AGG 0.458591 3_4:+14899891 Msa0491920:CDS
GCTAGTTAGGCTTTAGGAAA+AGG 0.460344 3_4:+14901515 Msa0491920:exon
ATGCCTATTCATAGGAGAAA+GGG 0.465580 3_4:+14901489 Msa0491920:CDS
CACCCCTTTCTCCTATGAAT+AGG 0.466798 3_4:-14901492 None:intergenic
TTCTTGCTATGCCTATTCAT+AGG 0.470964 3_4:+14901481 Msa0491920:CDS
GAAATCAGAAATATTGCAGC+TGG 0.472004 3_4:+14901123 Msa0491920:intron
ACCATGCTCATTATTACACT+TGG 0.488352 3_4:-14901580 None:intergenic
AAGCATACACCACTTGATAC+AGG 0.494102 3_4:-14901639 None:intergenic
ATTCCAGTGTCATTAAATAG+TGG 0.501376 3_4:+14901886 Msa0491920:three_prime_UTR
CAGCATGGCTGATCTGTTCA+AGG 0.501913 3_4:+14901822 Msa0491920:three_prime_UTR
CTCATCCTTGATGCGTGGCG+TGG 0.511920 3_4:-14899878 None:intergenic
CAAATCTCACAGCGCGAGCA+AGG 0.512938 3_4:+14899845 Msa0491920:CDS
AGAACTGGCATTTATACTCC+TGG 0.518145 3_4:-14901352 None:intergenic
TGCTCGCGCTGTGAGATTTG+GGG 0.527520 3_4:-14899842 Msa0491910:CDS
AAGCAAGAGTGAAGTTCTAT+GGG 0.532265 3_4:+14901859 Msa0491920:three_prime_UTR
AAAAGGAGAGGTTTAAGTCC+AGG 0.539612 3_4:+14901334 Msa0491920:CDS
TCATCCTTGATGCGTGGCGT+GGG 0.542840 3_4:-14899877 None:intergenic
GTCTCCCACGCCACGCATCA+AGG 0.546669 3_4:+14899873 Msa0491920:CDS
TATGCCTATTCATAGGAGAA+AGG 0.549487 3_4:+14901488 Msa0491920:CDS
TTCCAAATCATGCAATTACA+GGG 0.562181 3_4:-14901771 None:intergenic
TCACAGCGCGAGCAAGGGCA+TGG 0.564400 3_4:+14899851 Msa0491920:CDS
GTTTATGGATCCTCATTGGA+GGG 0.574530 3_4:+14901452 Msa0491920:CDS
ACCAAGTGTAATAATGAGCA+TGG 0.579867 3_4:+14901579 Msa0491920:three_prime_UTR
TGATAAATGCACTAGTAGCA+TGG 0.584691 3_4:+14901271 Msa0491920:CDS
TAGCCACTATTTAATGACAC+TGG 0.586478 3_4:-14901889 None:intergenic
AAATCTCACAGCGCGAGCAA+GGG 0.587168 3_4:+14899846 Msa0491920:CDS
AGTTTATGGATCCTCATTGG+AGG 0.600650 3_4:+14901451 Msa0491920:CDS
AGCATGGACTCAAGCATCTG+AGG 0.606559 3_4:+14901287 Msa0491920:CDS
TTGTATTTGCCTGTATCAAG+TGG 0.617368 3_4:+14901630 Msa0491920:three_prime_UTR
TGCCTATTCATAGGAGAAAG+GGG 0.636692 3_4:+14901490 Msa0491920:CDS
AGAAACACTTCCCTCCAATG+AGG 0.663156 3_4:-14901462 None:intergenic
CACCGCCGCGAAAAGCACCA+CGG 0.677236 3_4:-14899804 Msa0491910:CDS
TGTTACTCATCCTTGATGCG+TGG 0.694116 3_4:-14899883 None:intergenic
AGAGTACATTACGAAATCCG+TGG 0.707878 3_4:+14899787 None:intergenic
CTATTCATAGGAGAAAGGGG+TGG 0.735511 3_4:+14901493 Msa0491920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAAATATCTAAGATTGA+TGG - chr3_4:14899960-14899979 None:intergenic 15.0%
!! CATTATTCATTCAATAATTT+GGG - chr3_4:14900036-14900055 None:intergenic 15.0%
!! ACATTATTCATTCAATAATT+TGG - chr3_4:14900037-14900056 None:intergenic 15.0%
!! TGATTTCATATAACTTATAA+TGG - chr3_4:14901110-14901129 None:intergenic 15.0%
!!! ATAAAAATATCTAAGATTGA+TGG - chr3_4:14899960-14899979 None:intergenic 15.0%
!! CATTATTCATTCAATAATTT+GGG - chr3_4:14900036-14900055 None:intergenic 15.0%
!! ACATTATTCATTCAATAATT+TGG - chr3_4:14900037-14900056 None:intergenic 15.0%
!! TGATTTCATATAACTTATAA+TGG - chr3_4:14901110-14901129 None:intergenic 15.0%
!!! GATTTTATGCTTGAAATTAT+AGG + chr3_4:14899993-14900012 Msa0491920:intron 20.0%
!! TTACAATATTTACAATCAAG+TGG + chr3_4:14900180-14900199 Msa0491920:intron 20.0%
!!! CTAACTTAATCTTTTCTTTT+TGG - chr3_4:14900352-14900371 None:intergenic 20.0%
!! CAAAAAGAAAAGATTAAGTT+AGG + chr3_4:14900350-14900369 Msa0491920:intron 20.0%
!!! CCATCTAAGAATTTAAAATT+CGG - chr3_4:14900427-14900446 None:intergenic 20.0%
!! TTTGAAAATGTGTTCATAAA+TGG + chr3_4:14900600-14900619 Msa0491920:intron 20.0%
!! AACACTAAAATTATTAGCTA+TGG - chr3_4:14900650-14900669 None:intergenic 20.0%
!! TCAATTTCTCAAAATCTTAT+AGG + chr3_4:14900846-14900865 Msa0491920:intron 20.0%
!!! ATGATATGGTTTTGTTTAAA+TGG + chr3_4:14900962-14900981 Msa0491920:intron 20.0%
!! CAAAAACATTAAATTCAAAG+GGG - chr3_4:14901608-14901627 None:intergenic 20.0%
!! CCAAAAACATTAAATTCAAA+GGG - chr3_4:14901609-14901628 None:intergenic 20.0%
!! ACCAAAAACATTAAATTCAA+AGG - chr3_4:14901610-14901629 None:intergenic 20.0%
!!! TAATTCTAGTTTTGACATAA+AGG + chr3_4:14901736-14901755 Msa0491920:three_prime_UTR 20.0%
!!! GATTTTATGCTTGAAATTAT+AGG + chr3_4:14899993-14900012 Msa0491920:intron 20.0%
!! TTACAATATTTACAATCAAG+TGG + chr3_4:14900180-14900199 Msa0491920:intron 20.0%
!!! CTAACTTAATCTTTTCTTTT+TGG - chr3_4:14900352-14900371 None:intergenic 20.0%
!! CAAAAAGAAAAGATTAAGTT+AGG + chr3_4:14900350-14900369 Msa0491920:intron 20.0%
!!! CCATCTAAGAATTTAAAATT+CGG - chr3_4:14900427-14900446 None:intergenic 20.0%
!! TTTGAAAATGTGTTCATAAA+TGG + chr3_4:14900600-14900619 Msa0491920:intron 20.0%
!! AACACTAAAATTATTAGCTA+TGG - chr3_4:14900650-14900669 None:intergenic 20.0%
!! TCAATTTCTCAAAATCTTAT+AGG + chr3_4:14900846-14900865 Msa0491920:intron 20.0%
!!! ATGATATGGTTTTGTTTAAA+TGG + chr3_4:14900962-14900981 Msa0491920:intron 20.0%
!! CAAAAACATTAAATTCAAAG+GGG - chr3_4:14901608-14901627 None:intergenic 20.0%
!! CCAAAAACATTAAATTCAAA+GGG - chr3_4:14901609-14901628 None:intergenic 20.0%
!! ACCAAAAACATTAAATTCAA+AGG - chr3_4:14901610-14901629 None:intergenic 20.0%
!!! TAATTCTAGTTTTGACATAA+AGG + chr3_4:14901736-14901755 Msa0491920:three_prime_UTR 20.0%
! AAAGAAAACGTTGAATTTAG+AGG - chr3_4:14899921-14899940 None:intergenic 25.0%
!! AATATCTAAGATTGATGGTA+TGG - chr3_4:14899955-14899974 None:intergenic 25.0%
! ACAATTTGATACATGATACA+GGG + chr3_4:14900064-14900083 Msa0491920:intron 25.0%
! AATATTGTAAAACGTTACTC+AGG - chr3_4:14900170-14900189 None:intergenic 25.0%
!! CCGAATTTTAAATTCTTAGA+TGG + chr3_4:14900424-14900443 Msa0491920:intron 25.0%
! AAATTCTTAGATGGTAATCA+TGG + chr3_4:14900433-14900452 Msa0491920:intron 25.0%
!! ATACTCTTTACGTTTAGAAT+TGG - chr3_4:14900524-14900543 None:intergenic 25.0%
! TGCTTATTTATTATTAGCGA+TGG + chr3_4:14900554-14900573 Msa0491920:intron 25.0%
! GCTTATTTATTATTAGCGAT+GGG + chr3_4:14900555-14900574 Msa0491920:intron 25.0%
! AAAACTTCAGCTAAATGATA+TGG + chr3_4:14900948-14900967 Msa0491920:intron 25.0%
!! GCTGCTTAATTTTAAATGAA+AGG + chr3_4:14901078-14901097 Msa0491920:intron 25.0%
!!! AGCTTTTATTTTAGCTGAAA+AGG + chr3_4:14901317-14901336 Msa0491920:CDS 25.0%
!! CCCTTTGAATTTAATGTTTT+TGG + chr3_4:14901606-14901625 Msa0491920:three_prime_UTR 25.0%
! AAAGAAAACGTTGAATTTAG+AGG - chr3_4:14899921-14899940 None:intergenic 25.0%
!! AATATCTAAGATTGATGGTA+TGG - chr3_4:14899955-14899974 None:intergenic 25.0%
! ACAATTTGATACATGATACA+GGG + chr3_4:14900064-14900083 Msa0491920:intron 25.0%
! AATATTGTAAAACGTTACTC+AGG - chr3_4:14900170-14900189 None:intergenic 25.0%
!! CCGAATTTTAAATTCTTAGA+TGG + chr3_4:14900424-14900443 Msa0491920:intron 25.0%
! AAATTCTTAGATGGTAATCA+TGG + chr3_4:14900433-14900452 Msa0491920:intron 25.0%
!! ATACTCTTTACGTTTAGAAT+TGG - chr3_4:14900524-14900543 None:intergenic 25.0%
! TGCTTATTTATTATTAGCGA+TGG + chr3_4:14900554-14900573 Msa0491920:intron 25.0%
! GCTTATTTATTATTAGCGAT+GGG + chr3_4:14900555-14900574 Msa0491920:intron 25.0%
! AAAACTTCAGCTAAATGATA+TGG + chr3_4:14900948-14900967 Msa0491920:intron 25.0%
!! GCTGCTTAATTTTAAATGAA+AGG + chr3_4:14901078-14901097 Msa0491920:intron 25.0%
!!! AGCTTTTATTTTAGCTGAAA+AGG + chr3_4:14901317-14901336 Msa0491920:CDS 25.0%
!! CCCTTTGAATTTAATGTTTT+TGG + chr3_4:14901606-14901625 Msa0491920:three_prime_UTR 25.0%
CACAATTTGATACATGATAC+AGG + chr3_4:14900063-14900082 Msa0491920:intron 30.0%
CAATTTGATACATGATACAG+GGG + chr3_4:14900065-14900084 Msa0491920:intron 30.0%
TAATGATTTGAAGGTCATGA+TGG + chr3_4:14900124-14900143 Msa0491920:intron 30.0%
AATGATTTGAAGGTCATGAT+GGG + chr3_4:14900125-14900144 Msa0491920:intron 30.0%
! TCATTTGTTACGTTACAAGA+CGG + chr3_4:14900380-14900399 Msa0491920:intron 30.0%
GAAAATGTGTTCATAAATGG+TGG + chr3_4:14900603-14900622 Msa0491920:intron 30.0%
! AAGTCTTTGATTAGAAGTGT+TGG - chr3_4:14900775-14900794 None:intergenic 30.0%
AATGGTCAATATTTCTTGTC+TGG + chr3_4:14900980-14900999 Msa0491920:intron 30.0%
ATGGTCAATATTTCTTGTCT+GGG + chr3_4:14900981-14901000 Msa0491920:intron 30.0%
ATAGCTAAAGTGTATATAGC+TGG - chr3_4:14901044-14901063 None:intergenic 30.0%
CAAGAATCAACACAAATAGA+TGG + chr3_4:14901168-14901187 Msa0491920:CDS 30.0%
!!! TTATTTTAGCTGAAAAGGAG+AGG + chr3_4:14901322-14901341 Msa0491920:CDS 30.0%
GAAAGCTTGTGAAAAGTTTA+TGG + chr3_4:14901437-14901456 Msa0491920:CDS 30.0%
AAAAGTTTATGGATCCTCAT+TGG + chr3_4:14901448-14901467 Msa0491920:CDS 30.0%
! CTGCAATTCAAAGTATCTAT+AGG - chr3_4:14901541-14901560 None:intergenic 30.0%
CATTATTACACTTGGTTACT+AGG - chr3_4:14901575-14901594 None:intergenic 30.0%
TTCCAAATCATGCAATTACA+GGG - chr3_4:14901774-14901793 None:intergenic 30.0%
! CATGCTGTCTTTTTACATAA+AGG - chr3_4:14901809-14901828 None:intergenic 30.0%
CTTTATGTAAAAAGACAGCA+TGG + chr3_4:14901807-14901826 Msa0491920:three_prime_UTR 30.0%
! ATTCCAGTGTCATTAAATAG+TGG + chr3_4:14901886-14901905 Msa0491920:three_prime_UTR 30.0%
CACAATTTGATACATGATAC+AGG + chr3_4:14900063-14900082 Msa0491920:intron 30.0%
CAATTTGATACATGATACAG+GGG + chr3_4:14900065-14900084 Msa0491920:intron 30.0%
TAATGATTTGAAGGTCATGA+TGG + chr3_4:14900124-14900143 Msa0491920:intron 30.0%
AATGATTTGAAGGTCATGAT+GGG + chr3_4:14900125-14900144 Msa0491920:intron 30.0%
! TCATTTGTTACGTTACAAGA+CGG + chr3_4:14900380-14900399 Msa0491920:intron 30.0%
GAAAATGTGTTCATAAATGG+TGG + chr3_4:14900603-14900622 Msa0491920:intron 30.0%
! AAGTCTTTGATTAGAAGTGT+TGG - chr3_4:14900775-14900794 None:intergenic 30.0%
AATGGTCAATATTTCTTGTC+TGG + chr3_4:14900980-14900999 Msa0491920:intron 30.0%
ATGGTCAATATTTCTTGTCT+GGG + chr3_4:14900981-14901000 Msa0491920:intron 30.0%
ATAGCTAAAGTGTATATAGC+TGG - chr3_4:14901044-14901063 None:intergenic 30.0%
CAAGAATCAACACAAATAGA+TGG + chr3_4:14901168-14901187 Msa0491920:CDS 30.0%
!!! TTATTTTAGCTGAAAAGGAG+AGG + chr3_4:14901322-14901341 Msa0491920:CDS 30.0%
GAAAGCTTGTGAAAAGTTTA+TGG + chr3_4:14901437-14901456 Msa0491920:CDS 30.0%
AAAAGTTTATGGATCCTCAT+TGG + chr3_4:14901448-14901467 Msa0491920:CDS 30.0%
! CTGCAATTCAAAGTATCTAT+AGG - chr3_4:14901541-14901560 None:intergenic 30.0%
CATTATTACACTTGGTTACT+AGG - chr3_4:14901575-14901594 None:intergenic 30.0%
TTCCAAATCATGCAATTACA+GGG - chr3_4:14901774-14901793 None:intergenic 30.0%
! CATGCTGTCTTTTTACATAA+AGG - chr3_4:14901809-14901828 None:intergenic 30.0%
CTTTATGTAAAAAGACAGCA+TGG + chr3_4:14901807-14901826 Msa0491920:three_prime_UTR 30.0%
! ATTCCAGTGTCATTAAATAG+TGG + chr3_4:14901886-14901905 Msa0491920:three_prime_UTR 30.0%
GTTGAGGGTTAATGATTTGA+AGG + chr3_4:14900115-14900134 Msa0491920:intron 35.0%
!! TTCCTGGGTTGAACAATTTT+TGG + chr3_4:14900251-14900270 Msa0491920:intron 35.0%
GACAGAAGCTAAGTAAAATC+AGG - chr3_4:14900277-14900296 None:intergenic 35.0%
!! TCTTTTTGGTATTAGTCCTC+CGG - chr3_4:14900338-14900357 None:intergenic 35.0%
TCAAACCTGTTAATAAGCAG+AGG - chr3_4:14900585-14900604 None:intergenic 35.0%
ATTAGCTATGGTAGAGTGAA+AGG - chr3_4:14900638-14900657 None:intergenic 35.0%
TGTTTGTGTTAGTGCTTCAT+AGG + chr3_4:14900883-14900902 Msa0491920:intron 35.0%
! TTTAGCTGAAGTTTTCCACT+TGG - chr3_4:14900943-14900962 None:intergenic 35.0%
TGGTCAATATTTCTTGTCTG+GGG + chr3_4:14900982-14901001 Msa0491920:intron 35.0%
! GGGGTTATGATTTGATTTAG+TGG + chr3_4:14901001-14901020 Msa0491920:intron 35.0%
GAAATCAGAAATATTGCAGC+TGG + chr3_4:14901123-14901142 Msa0491920:intron 35.0%
TGATAAATGCACTAGTAGCA+TGG + chr3_4:14901271-14901290 Msa0491920:CDS 35.0%
ACACTCAGTAATTGAAGAAC+TGG - chr3_4:14901370-14901389 None:intergenic 35.0%
! TTCTTGCTATGCCTATTCAT+AGG + chr3_4:14901481-14901500 Msa0491920:CDS 35.0%
TATGCCTATTCATAGGAGAA+AGG + chr3_4:14901488-14901507 Msa0491920:CDS 35.0%
ATGCCTATTCATAGGAGAAA+GGG + chr3_4:14901489-14901508 Msa0491920:CDS 35.0%
ACCAAGTGTAATAATGAGCA+TGG + chr3_4:14901579-14901598 Msa0491920:three_prime_UTR 35.0%
ACCATGCTCATTATTACACT+TGG - chr3_4:14901583-14901602 None:intergenic 35.0%
TTGTATTTGCCTGTATCAAG+TGG + chr3_4:14901630-14901649 Msa0491920:three_prime_UTR 35.0%
AACCCTGTAATTGCATGATT+TGG + chr3_4:14901769-14901788 Msa0491920:three_prime_UTR 35.0%
CTTCCAAATCATGCAATTAC+AGG - chr3_4:14901775-14901794 None:intergenic 35.0%
! TGCATGATTTGGAAGTAAAC+TGG + chr3_4:14901780-14901799 Msa0491920:three_prime_UTR 35.0%
AAGCAAGAGTGAAGTTCTAT+GGG + chr3_4:14901859-14901878 Msa0491920:three_prime_UTR 35.0%
TAGCCACTATTTAATGACAC+TGG - chr3_4:14901892-14901911 None:intergenic 35.0%
GTTGAGGGTTAATGATTTGA+AGG + chr3_4:14900115-14900134 Msa0491920:intron 35.0%
!! TTCCTGGGTTGAACAATTTT+TGG + chr3_4:14900251-14900270 Msa0491920:intron 35.0%
GACAGAAGCTAAGTAAAATC+AGG - chr3_4:14900277-14900296 None:intergenic 35.0%
!! TCTTTTTGGTATTAGTCCTC+CGG - chr3_4:14900338-14900357 None:intergenic 35.0%
TCAAACCTGTTAATAAGCAG+AGG - chr3_4:14900585-14900604 None:intergenic 35.0%
ATTAGCTATGGTAGAGTGAA+AGG - chr3_4:14900638-14900657 None:intergenic 35.0%
TGTTTGTGTTAGTGCTTCAT+AGG + chr3_4:14900883-14900902 Msa0491920:intron 35.0%
! TTTAGCTGAAGTTTTCCACT+TGG - chr3_4:14900943-14900962 None:intergenic 35.0%
TGGTCAATATTTCTTGTCTG+GGG + chr3_4:14900982-14901001 Msa0491920:intron 35.0%
! GGGGTTATGATTTGATTTAG+TGG + chr3_4:14901001-14901020 Msa0491920:intron 35.0%
GAAATCAGAAATATTGCAGC+TGG + chr3_4:14901123-14901142 Msa0491920:intron 35.0%
TGATAAATGCACTAGTAGCA+TGG + chr3_4:14901271-14901290 Msa0491920:CDS 35.0%
ACACTCAGTAATTGAAGAAC+TGG - chr3_4:14901370-14901389 None:intergenic 35.0%
! TTCTTGCTATGCCTATTCAT+AGG + chr3_4:14901481-14901500 Msa0491920:CDS 35.0%
TATGCCTATTCATAGGAGAA+AGG + chr3_4:14901488-14901507 Msa0491920:CDS 35.0%
ATGCCTATTCATAGGAGAAA+GGG + chr3_4:14901489-14901508 Msa0491920:CDS 35.0%
ACCAAGTGTAATAATGAGCA+TGG + chr3_4:14901579-14901598 Msa0491920:three_prime_UTR 35.0%
ACCATGCTCATTATTACACT+TGG - chr3_4:14901583-14901602 None:intergenic 35.0%
TTGTATTTGCCTGTATCAAG+TGG + chr3_4:14901630-14901649 Msa0491920:three_prime_UTR 35.0%
AACCCTGTAATTGCATGATT+TGG + chr3_4:14901769-14901788 Msa0491920:three_prime_UTR 35.0%
CTTCCAAATCATGCAATTAC+AGG - chr3_4:14901775-14901794 None:intergenic 35.0%
! TGCATGATTTGGAAGTAAAC+TGG + chr3_4:14901780-14901799 Msa0491920:three_prime_UTR 35.0%
AAGCAAGAGTGAAGTTCTAT+GGG + chr3_4:14901859-14901878 Msa0491920:three_prime_UTR 35.0%
TAGCCACTATTTAATGACAC+TGG - chr3_4:14901892-14901911 None:intergenic 35.0%
CAAGGATGAGTAACAACAAC+AGG + chr3_4:14899891-14899910 Msa0491920:CDS 40.0%
TTGATACATGATACAGGGGT+TGG + chr3_4:14900069-14900088 Msa0491920:intron 40.0%
TGATACATGATACAGGGGTT+GGG + chr3_4:14900070-14900089 Msa0491920:intron 40.0%
GTGGTTAATGAACTCCACTA+AGG + chr3_4:14900199-14900218 Msa0491920:intron 40.0%
GGCTCTTGTAATTCGAAGTT+CGG - chr3_4:14900299-14900318 None:intergenic 40.0%
ATTAGTCCTCCGGATTAACA+GGG - chr3_4:14900328-14900347 None:intergenic 40.0%
TATTAGTCCTCCGGATTAAC+AGG - chr3_4:14900329-14900348 None:intergenic 40.0%
!! ACGTTACAAGACGGTTTTGT+AGG + chr3_4:14900389-14900408 Msa0491920:intron 40.0%
AGTAACACGTGTCAGACATT+CGG - chr3_4:14900800-14900819 None:intergenic 40.0%
TAGTGCTTCATAGGTTGCAA+CGG + chr3_4:14900892-14900911 Msa0491920:intron 40.0%
!! GGTTGCAACGGACTAAATTT+AGG + chr3_4:14900904-14900923 Msa0491920:intron 40.0%
AAAAGGAGAGGTTTAAGTCC+AGG + chr3_4:14901334-14901353 Msa0491920:CDS 40.0%
AGAACTGGCATTTATACTCC+TGG - chr3_4:14901355-14901374 None:intergenic 40.0%
AGTTTATGGATCCTCATTGG+AGG + chr3_4:14901451-14901470 Msa0491920:CDS 40.0%
GTTTATGGATCCTCATTGGA+GGG + chr3_4:14901452-14901471 Msa0491920:CDS 40.0%
TGCCTATTCATAGGAGAAAG+GGG + chr3_4:14901490-14901509 Msa0491920:CDS 40.0%
!! GCTAGTTAGGCTTTAGGAAA+AGG + chr3_4:14901515-14901534 Msa0491920:exon 40.0%
AAGCATACACCACTTGATAC+AGG - chr3_4:14901642-14901661 None:intergenic 40.0%
CAAGCAAGAGTGAAGTTCTA+TGG + chr3_4:14901858-14901877 Msa0491920:three_prime_UTR 40.0%
CAAGGATGAGTAACAACAAC+AGG + chr3_4:14899891-14899910 Msa0491920:CDS 40.0%
TTGATACATGATACAGGGGT+TGG + chr3_4:14900069-14900088 Msa0491920:intron 40.0%
TGATACATGATACAGGGGTT+GGG + chr3_4:14900070-14900089 Msa0491920:intron 40.0%
GTGGTTAATGAACTCCACTA+AGG + chr3_4:14900199-14900218 Msa0491920:intron 40.0%
GGCTCTTGTAATTCGAAGTT+CGG - chr3_4:14900299-14900318 None:intergenic 40.0%
ATTAGTCCTCCGGATTAACA+GGG - chr3_4:14900328-14900347 None:intergenic 40.0%
TATTAGTCCTCCGGATTAAC+AGG - chr3_4:14900329-14900348 None:intergenic 40.0%
!! ACGTTACAAGACGGTTTTGT+AGG + chr3_4:14900389-14900408 Msa0491920:intron 40.0%
AGTAACACGTGTCAGACATT+CGG - chr3_4:14900800-14900819 None:intergenic 40.0%
TAGTGCTTCATAGGTTGCAA+CGG + chr3_4:14900892-14900911 Msa0491920:intron 40.0%
!! GGTTGCAACGGACTAAATTT+AGG + chr3_4:14900904-14900923 Msa0491920:intron 40.0%
AAAAGGAGAGGTTTAAGTCC+AGG + chr3_4:14901334-14901353 Msa0491920:CDS 40.0%
AGAACTGGCATTTATACTCC+TGG - chr3_4:14901355-14901374 None:intergenic 40.0%
AGTTTATGGATCCTCATTGG+AGG + chr3_4:14901451-14901470 Msa0491920:CDS 40.0%
GTTTATGGATCCTCATTGGA+GGG + chr3_4:14901452-14901471 Msa0491920:CDS 40.0%
TGCCTATTCATAGGAGAAAG+GGG + chr3_4:14901490-14901509 Msa0491920:CDS 40.0%
!! GCTAGTTAGGCTTTAGGAAA+AGG + chr3_4:14901515-14901534 Msa0491920:exon 40.0%
AAGCATACACCACTTGATAC+AGG - chr3_4:14901642-14901661 None:intergenic 40.0%
CAAGCAAGAGTGAAGTTCTA+TGG + chr3_4:14901858-14901877 Msa0491920:three_prime_UTR 40.0%
TGTTACTCATCCTTGATGCG+TGG - chr3_4:14899886-14899905 None:intergenic 45.0%
!! AACTGGTTTGAGTGCTGTTG+AGG + chr3_4:14900099-14900118 Msa0491920:intron 45.0%
!! ACTGGTTTGAGTGCTGTTGA+GGG + chr3_4:14900100-14900119 Msa0491920:intron 45.0%
CCTGAACAATCTGTCCTTAG+TGG - chr3_4:14900216-14900235 None:intergenic 45.0%
CCACTAAGGACAGATTGTTC+AGG + chr3_4:14900213-14900232 Msa0491920:intron 45.0%
AGAACCTGAGTTCGATTCCT+GGG + chr3_4:14900236-14900255 Msa0491920:intron 45.0%
GGCCAAAAATTGTTCAACCC+AGG - chr3_4:14900256-14900275 None:intergenic 45.0%
TTAGTCCTCCGGATTAACAG+GGG - chr3_4:14900327-14900346 None:intergenic 45.0%
GCGTTCCTCTGCTTATTAAC+AGG + chr3_4:14900577-14900596 Msa0491920:intron 45.0%
!! TAGAAGTGTTGGAGCTTCAG+AGG - chr3_4:14900764-14900783 None:intergenic 45.0%
GGTGAGATTCAGAAACCAAG+TGG + chr3_4:14900925-14900944 Msa0491920:intron 45.0%
AGAAACACTTCCCTCCAATG+AGG - chr3_4:14901465-14901484 None:intergenic 45.0%
CACCCCTTTCTCCTATGAAT+AGG - chr3_4:14901495-14901514 None:intergenic 45.0%
CTATTCATAGGAGAAAGGGG+TGG + chr3_4:14901493-14901512 Msa0491920:CDS 45.0%
TGTTACTCATCCTTGATGCG+TGG - chr3_4:14899886-14899905 None:intergenic 45.0%
!! AACTGGTTTGAGTGCTGTTG+AGG + chr3_4:14900099-14900118 Msa0491920:intron 45.0%
!! ACTGGTTTGAGTGCTGTTGA+GGG + chr3_4:14900100-14900119 Msa0491920:intron 45.0%
CCTGAACAATCTGTCCTTAG+TGG - chr3_4:14900216-14900235 None:intergenic 45.0%
CCACTAAGGACAGATTGTTC+AGG + chr3_4:14900213-14900232 Msa0491920:intron 45.0%
AGAACCTGAGTTCGATTCCT+GGG + chr3_4:14900236-14900255 Msa0491920:intron 45.0%
GGCCAAAAATTGTTCAACCC+AGG - chr3_4:14900256-14900275 None:intergenic 45.0%
TTAGTCCTCCGGATTAACAG+GGG - chr3_4:14900327-14900346 None:intergenic 45.0%
GCGTTCCTCTGCTTATTAAC+AGG + chr3_4:14900577-14900596 Msa0491920:intron 45.0%
!! TAGAAGTGTTGGAGCTTCAG+AGG - chr3_4:14900764-14900783 None:intergenic 45.0%
GGTGAGATTCAGAAACCAAG+TGG + chr3_4:14900925-14900944 Msa0491920:intron 45.0%
AGAAACACTTCCCTCCAATG+AGG - chr3_4:14901465-14901484 None:intergenic 45.0%
CACCCCTTTCTCCTATGAAT+AGG - chr3_4:14901495-14901514 None:intergenic 45.0%
CTATTCATAGGAGAAAGGGG+TGG + chr3_4:14901493-14901512 Msa0491920:CDS 45.0%
! GAAATCCGTGGTGCTTTTCG+CGG + chr3_4:14899799-14899818 Msa0491920:CDS 50.0%
TTGCTCGCGCTGTGAGATTT+GGG - chr3_4:14899846-14899865 Msa0491910:CDS 50.0%
AAATCTCACAGCGCGAGCAA+GGG + chr3_4:14899846-14899865 Msa0491920:CDS 50.0%
GAGAACCTGAGTTCGATTCC+TGG + chr3_4:14900235-14900254 Msa0491920:intron 50.0%
TCAACCCAGGAATCGAACTC+AGG - chr3_4:14900243-14900262 None:intergenic 50.0%
GCCTCTTTCCCCTGTTAATC+CGG + chr3_4:14900316-14900335 Msa0491920:intron 50.0%
TCCGGATTAACAGGGGAAAG+AGG - chr3_4:14900320-14900339 None:intergenic 50.0%
TCTTTCCCCTGTTAATCCGG+AGG + chr3_4:14900319-14900338 Msa0491920:intron 50.0%
! TCATCCGTTATCGAGTTGCG+CGG + chr3_4:14900486-14900505 Msa0491920:intron 50.0%
AGCATGGACTCAAGCATCTG+AGG + chr3_4:14901287-14901306 Msa0491920:CDS 50.0%
CAGCATGGCTGATCTGTTCA+AGG + chr3_4:14901822-14901841 Msa0491920:three_prime_UTR 50.0%
! GAAATCCGTGGTGCTTTTCG+CGG + chr3_4:14899799-14899818 Msa0491920:CDS 50.0%
TTGCTCGCGCTGTGAGATTT+GGG - chr3_4:14899846-14899865 Msa0491910:CDS 50.0%
AAATCTCACAGCGCGAGCAA+GGG + chr3_4:14899846-14899865 Msa0491920:CDS 50.0%
GAGAACCTGAGTTCGATTCC+TGG + chr3_4:14900235-14900254 Msa0491920:intron 50.0%
TCAACCCAGGAATCGAACTC+AGG - chr3_4:14900243-14900262 None:intergenic 50.0%
GCCTCTTTCCCCTGTTAATC+CGG + chr3_4:14900316-14900335 Msa0491920:intron 50.0%
TCCGGATTAACAGGGGAAAG+AGG - chr3_4:14900320-14900339 None:intergenic 50.0%
TCTTTCCCCTGTTAATCCGG+AGG + chr3_4:14900319-14900338 Msa0491920:intron 50.0%
! TCATCCGTTATCGAGTTGCG+CGG + chr3_4:14900486-14900505 Msa0491920:intron 50.0%
AGCATGGACTCAAGCATCTG+AGG + chr3_4:14901287-14901306 Msa0491920:CDS 50.0%
CAGCATGGCTGATCTGTTCA+AGG + chr3_4:14901822-14901841 Msa0491920:three_prime_UTR 50.0%
TGCTCGCGCTGTGAGATTTG+GGG - chr3_4:14899845-14899864 Msa0491910:CDS 55.0%
CTTGCTCGCGCTGTGAGATT+TGG - chr3_4:14899847-14899866 Msa0491910:CDS 55.0%
CAAATCTCACAGCGCGAGCA+AGG + chr3_4:14899845-14899864 Msa0491920:CDS 55.0%
TCATCCTTGATGCGTGGCGT+GGG - chr3_4:14899880-14899899 None:intergenic 55.0%
! GGAGAAAGGGGTGGCTAGTT+AGG + chr3_4:14901502-14901521 Msa0491920:CDS 55.0%
! GGGGTGGCTAGTTAGGCTTT+AGG + chr3_4:14901509-14901528 Msa0491920:CDS 55.0%
TGCTCGCGCTGTGAGATTTG+GGG - chr3_4:14899845-14899864 Msa0491910:CDS 55.0%
CTTGCTCGCGCTGTGAGATT+TGG - chr3_4:14899847-14899866 Msa0491910:CDS 55.0%
CAAATCTCACAGCGCGAGCA+AGG + chr3_4:14899845-14899864 Msa0491920:CDS 55.0%
TCATCCTTGATGCGTGGCGT+GGG - chr3_4:14899880-14899899 None:intergenic 55.0%
! GGAGAAAGGGGTGGCTAGTT+AGG + chr3_4:14901502-14901521 Msa0491920:CDS 55.0%
! GGGGTGGCTAGTTAGGCTTT+AGG + chr3_4:14901509-14901528 Msa0491920:CDS 55.0%
! ATCCGTGGTGCTTTTCGCGG+CGG + chr3_4:14899802-14899821 Msa0491920:CDS 60.0%
CTCATCCTTGATGCGTGGCG+TGG - chr3_4:14899881-14899900 None:intergenic 60.0%
CAGGGGTTGGGTAGCTCAAC+TGG + chr3_4:14900082-14900101 Msa0491920:intron 60.0%
AGCGCCGCGCAACTCGATAA+CGG - chr3_4:14900493-14900512 None:intergenic 60.0%
! ATCCGTGGTGCTTTTCGCGG+CGG + chr3_4:14899802-14899821 Msa0491920:CDS 60.0%
CTCATCCTTGATGCGTGGCG+TGG - chr3_4:14899881-14899900 None:intergenic 60.0%
CAGGGGTTGGGTAGCTCAAC+TGG + chr3_4:14900082-14900101 Msa0491920:intron 60.0%
AGCGCCGCGCAACTCGATAA+CGG - chr3_4:14900493-14900512 None:intergenic 60.0%
!! CACCGCCGCGAAAAGCACCA+CGG - chr3_4:14899807-14899826 Msa0491910:CDS 65.0%
TCACAGCGCGAGCAAGGGCA+TGG + chr3_4:14899851-14899870 Msa0491920:CDS 65.0%
GTCTCCCACGCCACGCATCA+AGG + chr3_4:14899873-14899892 Msa0491920:CDS 65.0%
!! CACCGCCGCGAAAAGCACCA+CGG - chr3_4:14899807-14899826 Msa0491910:CDS 65.0%
TCACAGCGCGAGCAAGGGCA+TGG + chr3_4:14899851-14899870 Msa0491920:CDS 65.0%
GTCTCCCACGCCACGCATCA+AGG + chr3_4:14899873-14899892 Msa0491920:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 14899791 14901927 14899791 ID=Msa0491920;Name=Msa0491920
chr3_4 mRNA 14899791 14901927 14899791 ID=Msa0491920-mRNA-1;Parent=Msa0491920;Name=Msa0491920-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.5|1|0|0|2|397|173
chr3_4 exon 14899791 14899912 14899791 ID=Msa0491920-mRNA-1:exon:4401;Parent=Msa0491920-mRNA-1
chr3_4 exon 14901131 14901927 14901131 ID=Msa0491920-mRNA-1:exon:4402;Parent=Msa0491920-mRNA-1
chr3_4 CDS 14899791 14899912 14899791 ID=Msa0491920-mRNA-1:cds;Parent=Msa0491920-mRNA-1
chr3_4 CDS 14901131 14901530 14901131 ID=Msa0491920-mRNA-1:cds;Parent=Msa0491920-mRNA-1
chr3_4 three_prime_UTR 14901531 14901927 14901531 ID=Msa0491920-mRNA-1:three_prime_utr;Parent=Msa0491920-mRNA-1
Gene Sequence

>Msa0491920

TACATTACGAAATCCGTGGTGCTTTTCGCGGCGGTGCAAGAAGATCAATATCCCCAAATCTCACAGCGCGAGCAAGGGCATGGTCTCCCACGCCACGCATCAAGGATGAGTAACAACAACAGAAATATTGCAGCTGGAGTATTTCATTATTCATCTTCACAAGAATCAACACAAATAGATGGAGATAGTTCAGATGATAACACAGATTCACTGAATACAGTTGAAGATAGTTCAGATGAAAAAGAATATTCGAAAAATGCATTGATAAATGCACTAGTAGCATGGACTCAAGCATCTGAGGCAGAATTAGCTTTTATTTTAGCTGAAAAGGAGAGGTTTAAGTCCAGGAGTATAAATGCCAGTTCTTCAATTACTGAGTGTGTGACTGCTCTTGACAAGATTGAAGATGTTACAGATGACATTTATATGAAAGCTTGTGAAAAGTTTATGGATCCTCATTGGAGGGAAGTGTTTCTTGCTATGCCTATTCATAGGAGAAAGGGGTGGCTAGTTAGGCTTTAG
Protein sequence

>Msa0491920

YITKSVVLFAAVQEDQYPQISQREQGHGLPRHASRMSNNNRNIAAGVFHYSSSQESTQIDGDSSDDNTDSLNTVEDSSDEKEYSKNALINALVAWTQASEAELAFILAEKERFKSRSINASSSITECVTALDKIEDVTDDIYMKACEKFMDPHWREVFLAMPIHRRKGWLVRL*