AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0517170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517170 MtrunA17_Chr3g0128471 91.829 257 16 3 1 254 1 255 7.45e-167 461
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0517170 AT4G19985.6 48.052 231 89 5 17 223 14 237 3.25e-67 208
Msa0517170 AT4G19985.1 48.052 231 89 5 17 223 14 237 3.25e-67 208
Msa0517170 AT4G19985.4 42.205 263 89 6 17 223 14 269 5.05e-60 191
Msa0517170 AT4G19985.2 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa0517170 AT4G19985.5 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa0517170 AT4G19985.3 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa0517170 AT4G19985.7 39.181 171 73 5 17 163 14 177 1.90e-30 112

Find 83 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATTTATAAGAGAAATT+TGG 0.153536 3_4:-68662921 None:intergenic
CGAAATCAATGGAATTAAAA+TGG 0.164701 3_4:+68660539 Msa0517170:CDS
GGTCGTGCTGTTTCTGATTT+TGG 0.179788 3_4:+68660919 Msa0517170:CDS
CATTTAGGATATACCGATTT+AGG 0.186285 3_4:-68663108 None:intergenic
GTCCTATTTATAAGAAATTT+TGG 0.192709 3_4:+68662838 Msa0517170:three_prime_UTR
TGGATTTGGTGGTGACATTT+TGG 0.201084 3_4:+68662600 Msa0517170:CDS
CCCAAATTTCTCTTATAAAT+AGG 0.204007 3_4:+68662920 Msa0517170:three_prime_UTR
CTGAAATCTTTCTCACATTT+AGG 0.216532 3_4:-68663123 None:intergenic
TGACGGTTTGCTCACTTTCC+TGG 0.239344 3_4:-68662666 None:intergenic
TTTGGAGATGTTGAGGATTA+AGG 0.248880 3_4:-68660660 None:intergenic
TCAACAAAAGTTATTGTATC+TGG 0.258639 3_4:+68662861 Msa0517170:three_prime_UTR
CAGGAGTGTCGGAGATATTA+AGG 0.273878 3_4:-68660726 None:intergenic
TGCAAATGGAGCAGAGGTTT+TGG 0.278048 3_4:-68660678 None:intergenic
GACCAAAATTTCTTATAAAT+AGG 0.278339 3_4:-68662840 None:intergenic
CCTCACCACATTGATGTTGA+TGG 0.296205 3_4:+68660820 Msa0517170:CDS
CCTGAACGTGATTTGTTGAT+TGG 0.298630 3_4:+68660892 Msa0517170:CDS
CCTATTTATAAGAGAAATTT+GGG 0.322268 3_4:-68662920 None:intergenic
TCAACAAAAGTGGATGTATT+TGG 0.333075 3_4:-68662897 None:intergenic
ATTAAGGTGGTGTGGGTTAG+TGG 0.360151 3_4:-68660644 None:intergenic
AAAATTGTTGTTTAAGCAAC+CGG 0.364594 3_4:+68662797 Msa0517170:three_prime_UTR
TTGTTGATATCAACTGGTTC+AGG 0.374194 3_4:-68660745 None:intergenic
CGTGATTTGTTGATTGGGTT+TGG 0.384811 3_4:+68660898 Msa0517170:CDS
CGAAGCTTGTTGATATCAAC+TGG 0.398400 3_4:-68660751 None:intergenic
CTGAACGTGATTTGTTGATT+GGG 0.419104 3_4:+68660893 Msa0517170:CDS
TGCTTCTATATACGATGTTA+TGG 0.428860 3_4:+68660948 Msa0517170:CDS
GTACAGAGTATATCCTAAAT+CGG 0.430618 3_4:+68663095 Msa0517170:three_prime_UTR
CCAGAGGGAGTAACCTCTTC+CGG 0.431132 3_4:-68662816 None:intergenic
TATTCCATCCTTGAGGAGAA+TGG 0.433471 3_4:+68661566 Msa0517170:CDS
TCCTTGAGGAGAATGGGAAT+TGG 0.440922 3_4:+68661573 Msa0517170:CDS
CCGGAAGAGGTTACTCCCTC+TGG 0.452944 3_4:+68662816 Msa0517170:three_prime_UTR
ATTCCATCCTTGAGGAGAAT+GGG 0.454926 3_4:+68661567 Msa0517170:CDS
TTGTGTATATTCAACTGAGC+AGG 0.455019 3_4:+68662984 Msa0517170:three_prime_UTR
GTGTCGGAGATATTAAGGTT+GGG 0.458757 3_4:-68660721 None:intergenic
CCAATCAACAAATCACGTTC+AGG 0.463909 3_4:-68660892 None:intergenic
CCTCTCATCCTCTGAGCAAA+GGG 0.464970 3_4:-68662484 None:intergenic
GGAAATGAAAAGTGGGAGAA+GGG 0.466176 3_4:-68660623 None:intergenic
GAAATCAATGGAATTAAAAT+GGG 0.468872 3_4:+68660540 Msa0517170:CDS
AACAATAGCTTTCGGCCTGC+AGG 0.469985 3_4:-68662691 None:intergenic
AAATCACGTTCAGGAGAAAC+CGG 0.482910 3_4:-68660883 None:intergenic
TTTAAGGCTTGTGGATTTGG+TGG 0.483526 3_4:+68662589 Msa0517170:CDS
GAGCAAACCGTCACACCTGC+AGG 0.493036 3_4:+68662676 Msa0517170:CDS
GTTGTTTAAGCAACCGGAAG+AGG 0.493158 3_4:+68662803 Msa0517170:three_prime_UTR
TCCAATTCCCATTCTCCTCA+AGG 0.504293 3_4:-68661574 None:intergenic
GAAATTTGGGTCAACAAAAG+TGG 0.507540 3_4:-68662907 None:intergenic
GTGAAAAGAGTGGTTGCAAA+TGG 0.508457 3_4:-68660692 None:intergenic
GCGGAACCAACAATAGCTTT+CGG 0.512055 3_4:-68662699 None:intergenic
TGCAGGCCGAAAGCTATTGT+TGG 0.512613 3_4:+68662693 Msa0517170:CDS
AACTGTTTCATCAACAATCC+AGG 0.512965 3_4:+68662648 Msa0517170:CDS
ATCCATCCATCGAAAGTCGT+TGG 0.519423 3_4:+68660461 None:intergenic
TGTCGGAGATATTAAGGTTG+GGG 0.519931 3_4:-68660720 None:intergenic
ATGGTAACATTTACTCCCTC+CGG 0.522295 3_4:-68662944 None:intergenic
CTTTCGGCCTGCAGGTGTGA+CGG 0.523798 3_4:-68662683 None:intergenic
ACCTCTCATCCTCTGAGCAA+AGG 0.534812 3_4:-68662485 None:intergenic
CCCTTTGCTCAGAGGATGAG+AGG 0.537381 3_4:+68662484 Msa0517170:CDS
ATTTCTCTTATAAATAGGAC+CGG 0.540197 3_4:+68662925 Msa0517170:three_prime_UTR
AGTGTCGGAGATATTAAGGT+TGG 0.549755 3_4:-68660722 None:intergenic
TTCTTATAAATAGGACCAGA+GGG 0.553759 3_4:-68662831 None:intergenic
CTCTTATAAATAGGACCGGA+GGG 0.554392 3_4:+68662929 Msa0517170:three_prime_UTR
ATCAACTGGTTCAGGAGTGT+CGG 0.554412 3_4:-68660737 None:intergenic
TGTTGAGGATTAAGGTGGTG+TGG 0.555356 3_4:-68660652 None:intergenic
AACAGTTCTCGTGTACATCA+TGG 0.561270 3_4:-68662632 None:intergenic
GTTGAGGATTAAGGTGGTGT+GGG 0.570563 3_4:-68660651 None:intergenic
TGGAAATGAAAAGTGGGAGA+AGG 0.570598 3_4:-68660624 None:intergenic
ATTCCCATTCTCCTCAAGGA+TGG 0.579989 3_4:-68661570 None:intergenic
GGAGATGTTGAGGATTAAGG+TGG 0.587846 3_4:-68660657 None:intergenic
TGCAGGTTATTCCATCCTTG+AGG 0.587883 3_4:+68661559 Msa0517170:intron
ACTCAATATTATCATCCTTG+AGG 0.593636 3_4:-68662745 None:intergenic
TTTCTTATAAATAGGACCAG+AGG 0.595055 3_4:-68662832 None:intergenic
GGGTTAGTGGAAATGAAAAG+TGG 0.602812 3_4:-68660631 None:intergenic
GACAGATTCAAGAATCCTCA+AGG 0.611144 3_4:+68662730 Msa0517170:CDS
TCTCTTATAAATAGGACCGG+AGG 0.614852 3_4:+68662928 Msa0517170:three_prime_UTR
TGTGTATATTCAACTGAGCA+GGG 0.620117 3_4:+68662985 Msa0517170:three_prime_UTR
GAAATGAAAAGTGGGAGAAG+GGG 0.622002 3_4:-68660622 None:intergenic
CATAGCAGCCCTTTGCTCAG+AGG 0.625766 3_4:+68662476 Msa0517170:CDS
AGTGGTTGCAAATGGAGCAG+AGG 0.626606 3_4:-68660684 None:intergenic
TTTCGAAGCGAAAACTTCAG+TGG 0.633639 3_4:-68660521 None:intergenic
TTCTTGAATCTGTCAATCAG+AGG 0.641603 3_4:-68662721 None:intergenic
CCATCAACATCAATGTGGTG+AGG 0.651681 3_4:-68660820 None:intergenic
GGTTAGTGGAAATGAAAAGT+GGG 0.652172 3_4:-68660630 None:intergenic
TGGGGAATCTGTGAAAAGAG+TGG 0.656361 3_4:-68660702 None:intergenic
TTGAATCTGTCAATCAGAGG+CGG 0.671155 3_4:-68662718 None:intergenic
TGATTGTTAGAAAAGTTGTG+AGG 0.694957 3_4:+68661601 Msa0517170:CDS
TTAAACCATCAACATCAATG+TGG 0.739906 3_4:-68660825 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAATGTATAAATAA+AGG + chr3_4:68662010-68662029 Msa0517170:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + chr3_4:68662011-68662030 Msa0517170:intron 10.0%
!! AGAAAAATGTATAAATAAAG+GGG + chr3_4:68662012-68662031 Msa0517170:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG + chr3_4:68662075-68662094 Msa0517170:intron 15.0%
!! AATTAGAGTTATATTGTATT+GGG + chr3_4:68662076-68662095 Msa0517170:intron 15.0%
!! GAAATCAATGGAATTAAAAT+GGG + chr3_4:68660540-68660559 Msa0517170:CDS 20.0%
!!! TCTTTGTTTTGAAAAAATGT+AGG - chr3_4:68660988-68661007 None:intergenic 20.0%
!! CAAAATAACCAAAATAATGA+TGG - chr3_4:68661077-68661096 None:intergenic 20.0%
!!! TATTTTGGTTATTTTGTTTC+TGG + chr3_4:68661081-68661100 Msa0517170:intron 20.0%
!!! ATTTTGGTTATTTTGTTTCT+GGG + chr3_4:68661082-68661101 Msa0517170:intron 20.0%
!! ATTGTCAGTTATTGTAATTA+TGG - chr3_4:68661142-68661161 None:intergenic 20.0%
!!! GTTTTTCCTTTTTCAATATT+TGG + chr3_4:68661464-68661483 Msa0517170:intron 20.0%
!! AAAGTGGAATATATTGTATT+GGG + chr3_4:68662035-68662054 Msa0517170:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + chr3_4:68662108-68662127 Msa0517170:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + chr3_4:68662145-68662164 Msa0517170:intron 20.0%
!!! GTCCTATTTATAAGAAATTT+TGG + chr3_4:68662838-68662857 Msa0517170:three_prime_UTR 20.0%
!! GACCAAAATTTCTTATAAAT+AGG - chr3_4:68662843-68662862 None:intergenic 20.0%
!! ATTGTATCTGGTTAAAAAAT+TGG + chr3_4:68662873-68662892 Msa0517170:three_prime_UTR 20.0%
!! CCTATTTATAAGAGAAATTT+GGG - chr3_4:68662923-68662942 None:intergenic 20.0%
!! TCCTATTTATAAGAGAAATT+TGG - chr3_4:68662924-68662943 None:intergenic 20.0%
! CGAAATCAATGGAATTAAAA+TGG + chr3_4:68660539-68660558 Msa0517170:CDS 25.0%
! ATGAGTTCAATTTCATATCT+TGG + chr3_4:68661044-68661063 Msa0517170:intron 25.0%
!!! CTATAAGCTGTTTTCTATTT+TGG - chr3_4:68661345-68661364 None:intergenic 25.0%
! AATAAGTTGTTTATCCAAAC+AGG + chr3_4:68661398-68661417 Msa0517170:intron 25.0%
! ATAAGTTGTTTATCCAAACA+GGG + chr3_4:68661399-68661418 Msa0517170:intron 25.0%
!! ACATGTTTTTCATTAACAAC+AGG - chr3_4:68661441-68661460 None:intergenic 25.0%
! TCAATGCCAAATATTGAAAA+AGG - chr3_4:68661473-68661492 None:intergenic 25.0%
!! ATCATTCATGAAGTTTTTGT+TGG + chr3_4:68661740-68661759 Msa0517170:intron 25.0%
! TGAATTTAATCACAATGTGT+CGG + chr3_4:68661893-68661912 Msa0517170:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG + chr3_4:68662034-68662053 Msa0517170:intron 25.0%
!! TTGAAATGGCTCAATTATTT+TGG + chr3_4:68662122-68662141 Msa0517170:intron 25.0%
!! TGAAATGGCTCAATTATTTT+GGG + chr3_4:68662123-68662142 Msa0517170:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + chr3_4:68662144-68662163 Msa0517170:intron 25.0%
! TAAGTCAAATTAAACCTTGT+AGG + chr3_4:68662291-68662310 Msa0517170:intron 25.0%
! AATATAAAGGGTTGTGTATA+AGG + chr3_4:68662357-68662376 Msa0517170:intron 25.0%
! ATATAAAGGGTTGTGTATAA+GGG + chr3_4:68662358-68662377 Msa0517170:intron 25.0%
! AAGTTCCAAATATCTGAATA+TGG + chr3_4:68662414-68662433 Msa0517170:intron 25.0%
! ATAGACCATATTCAGATATT+TGG - chr3_4:68662422-68662441 None:intergenic 25.0%
! TTGTTCATTATTCCTATACA+TGG + chr3_4:68662507-68662526 Msa0517170:intron 25.0%
!!! TTTTCTCAGGTTGTTTTTTA+AGG + chr3_4:68662573-68662592 Msa0517170:intron 25.0%
!!! AAAATTGTTGTTTAAGCAAC+CGG + chr3_4:68662797-68662816 Msa0517170:three_prime_UTR 25.0%
! TCAACAAAAGTTATTGTATC+TGG + chr3_4:68662861-68662880 Msa0517170:three_prime_UTR 25.0%
! CCCAAATTTCTCTTATAAAT+AGG + chr3_4:68662920-68662939 Msa0517170:three_prime_UTR 25.0%
! ATTTCTCTTATAAATAGGAC+CGG + chr3_4:68662925-68662944 Msa0517170:three_prime_UTR 25.0%
!!! TTACCATTTTTTGTCATTCT+TGG + chr3_4:68662960-68662979 Msa0517170:three_prime_UTR 25.0%
!! TTTTTGTTTGTGTGTGGTTT+TGG - chr3_4:68660590-68660609 None:intergenic 30.0%
TTAAACCATCAACATCAATG+TGG - chr3_4:68660828-68660847 None:intergenic 30.0%
TGCTTCTATATACGATGTTA+TGG + chr3_4:68660948-68660967 Msa0517170:CDS 30.0%
!!! GTTTTGAAAAAATGTAGGCA+AGG - chr3_4:68660983-68661002 None:intergenic 30.0%
! CAATTTCATATCTTGGTTCT+TGG + chr3_4:68661051-68661070 Msa0517170:intron 30.0%
! TTTCTGGGTTTTCTTAGTTT+TGG + chr3_4:68661097-68661116 Msa0517170:intron 30.0%
!! TTCTGGGTTTTCTTAGTTTT+GGG + chr3_4:68661098-68661117 Msa0517170:intron 30.0%
ACTAACTATTTATGCCTGTT+TGG + chr3_4:68661201-68661220 Msa0517170:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG - chr3_4:68661218-68661237 None:intergenic 30.0%
AAACAGTCTCACAATACTTA+TGG - chr3_4:68661253-68661272 None:intergenic 30.0%
!! TAAGTATTGTGAGACTGTTT+TGG + chr3_4:68661253-68661272 Msa0517170:intron 30.0%
CAGCTTATAGCTTATACAAT+AGG + chr3_4:68661355-68661374 Msa0517170:intron 30.0%
TATAAGATATAAGCCCTGTT+TGG - chr3_4:68661415-68661434 None:intergenic 30.0%
!! CAATATTTGGCATTGATTTG+AGG + chr3_4:68661477-68661496 Msa0517170:intron 30.0%
TGATTGTTAGAAAAGTTGTG+AGG + chr3_4:68661601-68661620 Msa0517170:CDS 30.0%
ATTGAATCATCACAGAAGAT+AGG - chr3_4:68661644-68661663 None:intergenic 30.0%
ATTAGATTCTCATCAGCAAA+AGG - chr3_4:68661720-68661739 None:intergenic 30.0%
! TTTTTGTTGGCATAGAGTTT+TGG + chr3_4:68661753-68661772 Msa0517170:intron 30.0%
TGTATAAATAAAGGGGAAAG+TGG + chr3_4:68662019-68662038 Msa0517170:intron 30.0%
TGCAATAAAAGCTGTCATAT+TGG + chr3_4:68662238-68662257 Msa0517170:intron 30.0%
GCAATAAAAGCTGTCATATT+GGG + chr3_4:68662239-68662258 Msa0517170:intron 30.0%
CTTGTAGGAGTTGAATATAA+GGG + chr3_4:68662306-68662325 Msa0517170:intron 30.0%
TTGTAGGAGTTGAATATAAG+GGG + chr3_4:68662307-68662326 Msa0517170:intron 30.0%
ATTGATCTTTAACCAGAGAT+GGG - chr3_4:68662389-68662408 None:intergenic 30.0%
TATTGATCTTTAACCAGAGA+TGG - chr3_4:68662390-68662409 None:intergenic 30.0%
GATAAAACTAGACCATGTAT+AGG - chr3_4:68662522-68662541 None:intergenic 30.0%
CATCAATTACTGTTCACAAA+GGG - chr3_4:68662544-68662563 None:intergenic 30.0%
ACATCAATTACTGTTCACAA+AGG - chr3_4:68662545-68662564 None:intergenic 30.0%
!!! TTTTTTAAGGCTTGTGGATT+TGG + chr3_4:68662586-68662605 Msa0517170:CDS 30.0%
ACTCAATATTATCATCCTTG+AGG - chr3_4:68662748-68662767 None:intergenic 30.0%
TTCTTATAAATAGGACCAGA+GGG - chr3_4:68662834-68662853 None:intergenic 30.0%
TTTCTTATAAATAGGACCAG+AGG - chr3_4:68662835-68662854 None:intergenic 30.0%
TCAACAAAAGTGGATGTATT+TGG - chr3_4:68662900-68662919 None:intergenic 30.0%
AAGCCAAGAATGACAAAAAA+TGG - chr3_4:68662966-68662985 None:intergenic 30.0%
GTACAGAGTATATCCTAAAT+CGG + chr3_4:68663095-68663114 Msa0517170:three_prime_UTR 30.0%
!! CATTTAGGATATACCGATTT+AGG - chr3_4:68663111-68663130 None:intergenic 30.0%
CTGAAATCTTTCTCACATTT+AGG - chr3_4:68663126-68663145 None:intergenic 30.0%
! AGTTTTCGCTTCGAAATCAA+TGG + chr3_4:68660528-68660547 Msa0517170:CDS 35.0%
!!! TAGCGTTTTTTGTTTGTGTG+TGG - chr3_4:68660596-68660615 None:intergenic 35.0%
GGTTAGTGGAAATGAAAAGT+GGG - chr3_4:68660633-68660652 None:intergenic 35.0%
TTTGGAGATGTTGAGGATTA+AGG - chr3_4:68660663-68660682 None:intergenic 35.0%
! TTGTTGATATCAACTGGTTC+AGG - chr3_4:68660748-68660767 None:intergenic 35.0%
!!! CATTGTTGATTTTTTCACGC+CGG + chr3_4:68660864-68660883 Msa0517170:CDS 35.0%
!! CTGAACGTGATTTGTTGATT+GGG + chr3_4:68660893-68660912 Msa0517170:CDS 35.0%
! GTTCTTGGCCATCATTATTT+TGG + chr3_4:68661066-68661085 Msa0517170:intron 35.0%
TTCTGTGATGATTCAATGCA+TGG + chr3_4:68661647-68661666 Msa0517170:intron 35.0%
! TGAAAGTGAGTATCTAGTTC+AGG - chr3_4:68661675-68661694 None:intergenic 35.0%
AATCTGTGTGAAAACCAAAC+TGG - chr3_4:68661991-68662010 None:intergenic 35.0%
AGAAAATGGGTCAGATATTC+CGG + chr3_4:68662158-68662177 Msa0517170:intron 35.0%
CCTTATATTCAACTCCTACA+AGG - chr3_4:68662308-68662327 None:intergenic 35.0%
CCTTGTAGGAGTTGAATATA+AGG + chr3_4:68662305-68662324 Msa0517170:intron 35.0%
!!! AGGTTGTTTTTTAAGGCTTG+TGG + chr3_4:68662580-68662599 Msa0517170:intron 35.0%
AACTGTTTCATCAACAATCC+AGG + chr3_4:68662648-68662667 Msa0517170:CDS 35.0%
TTCTTGAATCTGTCAATCAG+AGG - chr3_4:68662724-68662743 None:intergenic 35.0%
GAAATTTGGGTCAACAAAAG+TGG - chr3_4:68662910-68662929 None:intergenic 35.0%
TTGTGTATATTCAACTGAGC+AGG + chr3_4:68662984-68663003 Msa0517170:three_prime_UTR 35.0%
TGTGTATATTCAACTGAGCA+GGG + chr3_4:68662985-68663004 Msa0517170:three_prime_UTR 35.0%
! TCGTTGGTTGCAGACAAAAA+TGG + chr3_4:68660477-68660496 Msa0517170:exon 40.0%
TTGGTTGCAGACAAAAATGG+CGG + chr3_4:68660480-68660499 Msa0517170:exon 40.0%
TTTCGAAGCGAAAACTTCAG+TGG - chr3_4:68660524-68660543 None:intergenic 40.0%
GAAATGAAAAGTGGGAGAAG+GGG - chr3_4:68660625-68660644 None:intergenic 40.0%
GGAAATGAAAAGTGGGAGAA+GGG - chr3_4:68660626-68660645 None:intergenic 40.0%
TGGAAATGAAAAGTGGGAGA+AGG - chr3_4:68660627-68660646 None:intergenic 40.0%
! GGGTTAGTGGAAATGAAAAG+TGG - chr3_4:68660634-68660653 None:intergenic 40.0%
GTGAAAAGAGTGGTTGCAAA+TGG - chr3_4:68660695-68660714 None:intergenic 40.0%
TGTCGGAGATATTAAGGTTG+GGG - chr3_4:68660723-68660742 None:intergenic 40.0%
GTGTCGGAGATATTAAGGTT+GGG - chr3_4:68660724-68660743 None:intergenic 40.0%
! AGTGTCGGAGATATTAAGGT+TGG - chr3_4:68660725-68660744 None:intergenic 40.0%
!! CGAAGCTTGTTGATATCAAC+TGG - chr3_4:68660754-68660773 None:intergenic 40.0%
CGGCGTGAAAAAATCAACAA+TGG - chr3_4:68660866-68660885 None:intergenic 40.0%
AAATCACGTTCAGGAGAAAC+CGG - chr3_4:68660886-68660905 None:intergenic 40.0%
CCAATCAACAAATCACGTTC+AGG - chr3_4:68660895-68660914 None:intergenic 40.0%
!! CCTGAACGTGATTTGTTGAT+TGG + chr3_4:68660892-68660911 Msa0517170:CDS 40.0%
!! CGTGATTTGTTGATTGGGTT+TGG + chr3_4:68660898-68660917 Msa0517170:CDS 40.0%
! TTGCGTTTTCCCATAGTTGA+CGG + chr3_4:68661510-68661529 Msa0517170:intron 40.0%
GAATAATGCCCGTCAACTAT+GGG - chr3_4:68661522-68661541 None:intergenic 40.0%
TGAATAATGCCCGTCAACTA+TGG - chr3_4:68661523-68661542 None:intergenic 40.0%
TATTCCATCCTTGAGGAGAA+TGG + chr3_4:68661566-68661585 Msa0517170:CDS 40.0%
ATTCCATCCTTGAGGAGAAT+GGG + chr3_4:68661567-68661586 Msa0517170:CDS 40.0%
CATCACAGAAGATAGGCAAA+AGG - chr3_4:68661637-68661656 None:intergenic 40.0%
! TCTACTATGAGGCACTAACA+CGG + chr3_4:68661814-68661833 Msa0517170:intron 40.0%
GTCGATGTGTAAGTGTGTAT+TGG - chr3_4:68661843-68661862 None:intergenic 40.0%
GAAAATGGGTCAGATATTCC+GGG + chr3_4:68662159-68662178 Msa0517170:intron 40.0%
!! GATGTTCTCGACTTTTTCTC+AGG + chr3_4:68662560-68662579 Msa0517170:intron 40.0%
! TTTAAGGCTTGTGGATTTGG+TGG + chr3_4:68662589-68662608 Msa0517170:CDS 40.0%
!!! TGGATTTGGTGGTGACATTT+TGG + chr3_4:68662600-68662619 Msa0517170:CDS 40.0%
AACAGTTCTCGTGTACATCA+TGG - chr3_4:68662635-68662654 None:intergenic 40.0%
TTGAATCTGTCAATCAGAGG+CGG - chr3_4:68662721-68662740 None:intergenic 40.0%
GACAGATTCAAGAATCCTCA+AGG + chr3_4:68662730-68662749 Msa0517170:CDS 40.0%
TCTCTTATAAATAGGACCGG+AGG + chr3_4:68662928-68662947 Msa0517170:three_prime_UTR 40.0%
CTCTTATAAATAGGACCGGA+GGG + chr3_4:68662929-68662948 Msa0517170:three_prime_UTR 40.0%
ATGGTAACATTTACTCCCTC+CGG - chr3_4:68662947-68662966 None:intergenic 40.0%
!! ATTAAGGTGGTGTGGGTTAG+TGG - chr3_4:68660647-68660666 None:intergenic 45.0%
GTTGAGGATTAAGGTGGTGT+GGG - chr3_4:68660654-68660673 None:intergenic 45.0%
TGTTGAGGATTAAGGTGGTG+TGG - chr3_4:68660655-68660674 None:intergenic 45.0%
GGAGATGTTGAGGATTAAGG+TGG - chr3_4:68660660-68660679 None:intergenic 45.0%
!!! CAGAGGTTTTGGAGATGTTG+AGG - chr3_4:68660670-68660689 None:intergenic 45.0%
! TGCAAATGGAGCAGAGGTTT+TGG - chr3_4:68660681-68660700 None:intergenic 45.0%
TGGGGAATCTGTGAAAAGAG+TGG - chr3_4:68660705-68660724 None:intergenic 45.0%
!! CAGGAGTGTCGGAGATATTA+AGG - chr3_4:68660729-68660748 None:intergenic 45.0%
! ATCAACTGGTTCAGGAGTGT+CGG - chr3_4:68660740-68660759 None:intergenic 45.0%
CCATCAACATCAATGTGGTG+AGG - chr3_4:68660823-68660842 None:intergenic 45.0%
CCTCACCACATTGATGTTGA+TGG + chr3_4:68660820-68660839 Msa0517170:CDS 45.0%
!!! GGTCGTGCTGTTTCTGATTT+TGG + chr3_4:68660919-68660938 Msa0517170:CDS 45.0%
! TGCGTTTTCCCATAGTTGAC+GGG + chr3_4:68661511-68661530 Msa0517170:intron 45.0%
AACATGCTTCAGTGTCTTGC+AGG + chr3_4:68661542-68661561 Msa0517170:intron 45.0%
TGCAGGTTATTCCATCCTTG+AGG + chr3_4:68661559-68661578 Msa0517170:intron 45.0%
ATTCCCATTCTCCTCAAGGA+TGG - chr3_4:68661573-68661592 None:intergenic 45.0%
TCCTTGAGGAGAATGGGAAT+TGG + chr3_4:68661573-68661592 Msa0517170:CDS 45.0%
TCCAATTCCCATTCTCCTCA+AGG - chr3_4:68661577-68661596 None:intergenic 45.0%
TCACGTCAGTCTCTACTATG+AGG + chr3_4:68661803-68661822 Msa0517170:intron 45.0%
! ACGCCATCAATTAGAAGCGT+TGG + chr3_4:68661931-68661950 Msa0517170:intron 45.0%
GCACCAACGCTTCTAATTGA+TGG - chr3_4:68661937-68661956 None:intergenic 45.0%
!! CAAACTGGCTCGGATAAAAC+GGG - chr3_4:68661976-68661995 None:intergenic 45.0%
!! CCAAACTGGCTCGGATAAAA+CGG - chr3_4:68661977-68661996 None:intergenic 45.0%
GTGTGAAAACCAAACTGGCT+CGG - chr3_4:68661986-68662005 None:intergenic 45.0%
ATAAGGGTTCTTCCCATCTC+TGG + chr3_4:68662374-68662393 Msa0517170:intron 45.0%
GCGGAACCAACAATAGCTTT+CGG - chr3_4:68662702-68662721 None:intergenic 45.0%
GTTGTTTAAGCAACCGGAAG+AGG + chr3_4:68662803-68662822 Msa0517170:three_prime_UTR 45.0%
!!! ATTTGTTTTTTTTTTTAAAT+TGG - chr3_4:68662340-68662359 None:intergenic 5.0%
!! AAAAAAAAAAACAAATATAA+AGG + chr3_4:68662344-68662363 Msa0517170:intron 5.0%
!! AAAAAAAAAACAAATATAAA+GGG + chr3_4:68662345-68662364 Msa0517170:intron 5.0%
CAGACAAAAATGGCGGCAAC+AGG + chr3_4:68660487-68660506 Msa0517170:exon 50.0%
AGTGGTTGCAAATGGAGCAG+AGG - chr3_4:68660687-68660706 None:intergenic 50.0%
GTAGAGACTGACGTGAGTGA+GGG - chr3_4:68661799-68661818 None:intergenic 50.0%
AGTAGAGACTGACGTGAGTG+AGG - chr3_4:68661800-68661819 None:intergenic 50.0%
! CCGTTTTATCCGAGCCAGTT+TGG + chr3_4:68661974-68661993 Msa0517170:intron 50.0%
ATGGGTCAGATATTCCGGGA+CGG + chr3_4:68662163-68662182 Msa0517170:intron 50.0%
CCTCTCATCCTCTGAGCAAA+GGG - chr3_4:68662487-68662506 None:intergenic 50.0%
ACCTCTCATCCTCTGAGCAA+AGG - chr3_4:68662488-68662507 None:intergenic 50.0%
! TGACGGTTTGCTCACTTTCC+TGG - chr3_4:68662669-68662688 None:intergenic 50.0%
AACAATAGCTTTCGGCCTGC+AGG - chr3_4:68662694-68662713 None:intergenic 50.0%
TGCAGGCCGAAAGCTATTGT+TGG + chr3_4:68662693-68662712 Msa0517170:CDS 50.0%
CTCGGATAAAACGGGACGGA+GGG - chr3_4:68661968-68661987 None:intergenic 55.0%
!! CTGGCTCGGATAAAACGGGA+CGG - chr3_4:68661972-68661991 None:intergenic 55.0%
GTCAGATATTCCGGGACGGA+GGG + chr3_4:68662167-68662186 Msa0517170:intron 55.0%
GACGGAGGGAGTAGTTCTCT+AGG + chr3_4:68662181-68662200 Msa0517170:intron 55.0%
CATAGCAGCCCTTTGCTCAG+AGG + chr3_4:68662476-68662495 Msa0517170:CDS 55.0%
CCCTTTGCTCAGAGGATGAG+AGG + chr3_4:68662484-68662503 Msa0517170:CDS 55.0%
CCAGAGGGAGTAACCTCTTC+CGG - chr3_4:68662819-68662838 None:intergenic 55.0%
GCTCGGATAAAACGGGACGG+AGG - chr3_4:68661969-68661988 None:intergenic 60.0%
GGTCAGATATTCCGGGACGG+AGG + chr3_4:68662166-68662185 Msa0517170:intron 60.0%
GAGAACTACTCCCTCCGTCC+CGG - chr3_4:68662180-68662199 None:intergenic 60.0%
GAGCAAACCGTCACACCTGC+AGG + chr3_4:68662676-68662695 Msa0517170:CDS 60.0%
CTTTCGGCCTGCAGGTGTGA+CGG - chr3_4:68662686-68662705 None:intergenic 60.0%
CCGGAAGAGGTTACTCCCTC+TGG + chr3_4:68662816-68662835 Msa0517170:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 68660471 68663155 68660471 ID=Msa0517170;Name=Msa0517170
chr3_4 mRNA 68660471 68663155 68660471 ID=Msa0517170-mRNA-1;Parent=Msa0517170;Name=Msa0517170-mRNA-1;_AED=0.25;_eAED=0.25;_QI=25|1|1|1|1|1|4|399|254
chr3_4 exon 68660471 68660969 68660471 ID=Msa0517170-mRNA-1:exon:18165;Parent=Msa0517170-mRNA-1
chr3_4 exon 68661564 68661622 68661564 ID=Msa0517170-mRNA-1:exon:18166;Parent=Msa0517170-mRNA-1
chr3_4 exon 68662449 68662505 68662449 ID=Msa0517170-mRNA-1:exon:18167;Parent=Msa0517170-mRNA-1
chr3_4 exon 68662582 68663155 68662582 ID=Msa0517170-mRNA-1:exon:18168;Parent=Msa0517170-mRNA-1
chr3_4 five_prime_UTR 68660471 68660495 68660471 ID=Msa0517170-mRNA-1:five_prime_utr;Parent=Msa0517170-mRNA-1
chr3_4 CDS 68660496 68660969 68660496 ID=Msa0517170-mRNA-1:cds;Parent=Msa0517170-mRNA-1
chr3_4 CDS 68661564 68661622 68661564 ID=Msa0517170-mRNA-1:cds;Parent=Msa0517170-mRNA-1
chr3_4 CDS 68662449 68662505 68662449 ID=Msa0517170-mRNA-1:cds;Parent=Msa0517170-mRNA-1
chr3_4 CDS 68662582 68662756 68662582 ID=Msa0517170-mRNA-1:cds;Parent=Msa0517170-mRNA-1
chr3_4 three_prime_UTR 68662757 68663155 68662757 ID=Msa0517170-mRNA-1:three_prime_utr;Parent=Msa0517170-mRNA-1
Gene Sequence

>Msa0517170

ATGGCGGCAACAGGAGTTGCAGCAGCCACTGAAGTTTTCGCTTCGAAATCAATGGAATTAAAATGGGTAAGAACAAGAACAAGAAGAATCACCAAAACCACACACAAACAAAAAACGCTATCAACACCCCTTCTCCCACTTTTCATTTCCACTAACCCACACCACCTTAATCCTCAACATCTCCAAAACCTCTGCTCCATTTGCAACCACTCTTTTCACAGATTCCCCAACCTTAATATCTCCGACACTCCTGAACCAGTTGATATCAACAAGCTTCGCATTGCTCTTTCTCATAGTGACGTTCTTGTCTCTGTCTTCTGTAAACCTCACCACATTGATGTTGATGGTTTAACTGAATCTTCATCTTCCATTGTTGATTTTTTCACGCCGGTTTCTCCTGAACGTGATTTGTTGATTGGGTTTGGTCGTGCTGTTTCTGATTTTGGTCTCACTGCTTCTATATACGATGTTATGGTTATTCCATCCTTGAGGAGAATGGGAATTGGAAAAATGATTGTTAGAAAAGTTGTGAGAATGCTTACAAATAGAGACATCTATGACATAGCAGCCCTTTGCTCAGAGGATGAGAGGTTGTTTTTTAAGGCTTGTGGATTTGGTGGTGACATTTTGGACTCTACTACCATGATGTACACGAGAACTGTTTCATCAACAATCCAGGAAAGTGAGCAAACCGTCACACCTGCAGGCCGAAAGCTATTGTTGGTTCCGCCTCTGATTGACAGATTCAAGAATCCTCAAGGATGA
Protein sequence

>Msa0517170

MAATGVAAATEVFASKSMELKWVRTRTRRITKTTHKQKTLSTPLLPLFISTNPHHLNPQHLQNLCSICNHSFHRFPNLNISDTPEPVDINKLRIALSHSDVLVSVFCKPHHIDVDGLTESSSSIVDFFTPVSPERDLLIGFGRAVSDFGLTASIYDVMVIPSLRRMGIGKMIVRKVVRMLTNRDIYDIAALCSEDERLFFKACGFGGDILDSTTMMYTRTVSSTIQESEQTVTPAGRKLLLVPPLIDRFKNPQG*