AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393700 MtrunA17_Chr3g0128471 91.829 257 16 3 1 254 1 255 7.45e-167 461
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393700 AT4G19985.6 48.052 231 89 5 17 223 14 237 3.25e-67 208
Msa1393700 AT4G19985.1 48.052 231 89 5 17 223 14 237 3.25e-67 208
Msa1393700 AT4G19985.4 42.205 263 89 6 17 223 14 269 5.05e-60 191
Msa1393700 AT4G19985.2 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa1393700 AT4G19985.5 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa1393700 AT4G19985.3 39.881 168 70 5 17 160 14 174 1.60e-30 112
Msa1393700 AT4G19985.7 39.181 171 73 5 17 163 14 177 1.90e-30 112

Find 83 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTATTTATAAGAGAAATTT+TGG 0.134317 tig0021758:+12323 Msa1393700:three_prime_UTR
TCCTATTTATAAGAGAAATT+TGG 0.153536 tig0021758:-12406 None:intergenic
CGAAATCAATGGAATTAAAA+TGG 0.164701 tig0021758:+10021 Msa1393700:CDS
CCAAAATTTCTCTTATAAAT+AGG 0.165614 tig0021758:-12323 None:intergenic
GGTCGTGCTGTTTCTGATTT+TGG 0.179788 tig0021758:+10401 Msa1393700:CDS
CATTTAGGATATACCGATTT+AGG 0.186285 tig0021758:-12593 None:intergenic
TGGATTTGGTGGTGACATTT+TGG 0.201084 tig0021758:+12083 Msa1393700:CDS
CCCAAATTTCTCTTATAAAT+AGG 0.204007 tig0021758:+12405 Msa1393700:three_prime_UTR
CTGAAATCTTTCTCACATTT+AGG 0.216532 tig0021758:-12608 None:intergenic
TGACGGTTTGCTCACTTTCC+TGG 0.239344 tig0021758:-12149 None:intergenic
TTTGGAGATGTTGAGGATTA+AGG 0.248880 tig0021758:-10142 None:intergenic
TCAACAAAAGTTATTGTATC+TGG 0.258639 tig0021758:+12346 Msa1393700:three_prime_UTR
CAGGAGTGTCGGAGATATTA+AGG 0.273878 tig0021758:-10208 None:intergenic
TGCAAATGGAGCAGAGGTTT+TGG 0.278048 tig0021758:-10160 None:intergenic
CCTCACCACATTGATGTTGA+TGG 0.296205 tig0021758:+10302 Msa1393700:CDS
CCTGAACGTGATTTGTTGAT+TGG 0.298630 tig0021758:+10374 Msa1393700:CDS
CCTATTTATAAGAGAAATTT+GGG 0.322268 tig0021758:-12405 None:intergenic
TCAACAAAAGTGGATGTATT+TGG 0.333075 tig0021758:-12382 None:intergenic
ATTAAGGTGGTGTGGGTTAG+TGG 0.360151 tig0021758:-10126 None:intergenic
AAAATTGTTGTTTAAGCAAC+CGG 0.364594 tig0021758:+12280 Msa1393700:three_prime_UTR
TTGTTGATATCAACTGGTTC+AGG 0.374194 tig0021758:-10227 None:intergenic
CGTGATTTGTTGATTGGGTT+TGG 0.384811 tig0021758:+10380 Msa1393700:CDS
CGAAGCTTGTTGATATCAAC+TGG 0.398400 tig0021758:-10233 None:intergenic
CTGAACGTGATTTGTTGATT+GGG 0.419104 tig0021758:+10375 Msa1393700:CDS
TGCTTCTATATACGATGTTA+TGG 0.428860 tig0021758:+10430 Msa1393700:CDS
GTACAGAGTATATCCTAAAT+CGG 0.430618 tig0021758:+12580 Msa1393700:three_prime_UTR
CCAGAGGGAGTAACCTCTTC+CGG 0.431132 tig0021758:-12299 None:intergenic
TATTCCATCCTTGAGGAGAA+TGG 0.433471 tig0021758:+11049 Msa1393700:CDS
TCCTTGAGGAGAATGGGAAT+TGG 0.440922 tig0021758:+11056 Msa1393700:CDS
CCGGAAGAGGTTACTCCCTC+TGG 0.452944 tig0021758:+12299 Msa1393700:three_prime_UTR
ATTCCATCCTTGAGGAGAAT+GGG 0.454926 tig0021758:+11050 Msa1393700:CDS
TTGTGTATATTCAACTGAGC+AGG 0.455019 tig0021758:+12469 Msa1393700:three_prime_UTR
GTGTCGGAGATATTAAGGTT+GGG 0.458757 tig0021758:-10203 None:intergenic
CCAATCAACAAATCACGTTC+AGG 0.463909 tig0021758:-10374 None:intergenic
CCTCTCATCCTCTGAGCAAA+GGG 0.464970 tig0021758:-11967 None:intergenic
GGAAATGAAAAGTGGGAGAA+GGG 0.466176 tig0021758:-10105 None:intergenic
GAAATCAATGGAATTAAAAT+GGG 0.468872 tig0021758:+10022 Msa1393700:CDS
AACAATAGCTTTCGGCCTGC+AGG 0.469985 tig0021758:-12174 None:intergenic
AAATCACGTTCAGGAGAAAC+CGG 0.482910 tig0021758:-10365 None:intergenic
TTTAAGGCTTGTGGATTTGG+TGG 0.483526 tig0021758:+12072 Msa1393700:CDS
GAGCAAACCGTCACACCTGC+AGG 0.493036 tig0021758:+12159 Msa1393700:CDS
GTTGTTTAAGCAACCGGAAG+AGG 0.493158 tig0021758:+12286 Msa1393700:three_prime_UTR
TCCAATTCCCATTCTCCTCA+AGG 0.504293 tig0021758:-11057 None:intergenic
GAAATTTGGGTCAACAAAAG+TGG 0.507540 tig0021758:-12392 None:intergenic
GTGAAAAGAGTGGTTGCAAA+TGG 0.508457 tig0021758:-10174 None:intergenic
GCGGAACCAACAATAGCTTT+CGG 0.512055 tig0021758:-12182 None:intergenic
TGCAGGCCGAAAGCTATTGT+TGG 0.512613 tig0021758:+12176 Msa1393700:CDS
AACTGTTTCATCAACAATCC+AGG 0.512965 tig0021758:+12131 Msa1393700:CDS
ATCCATCCATCGAAAGTCGT+TGG 0.519423 tig0021758:+9943 None:intergenic
TGTCGGAGATATTAAGGTTG+GGG 0.519931 tig0021758:-10202 None:intergenic
ATGGTAACATTTACTCCCTC+CGG 0.522295 tig0021758:-12429 None:intergenic
CTTTCGGCCTGCAGGTGTGA+CGG 0.523798 tig0021758:-12166 None:intergenic
ACCTCTCATCCTCTGAGCAA+AGG 0.534812 tig0021758:-11968 None:intergenic
CCCTTTGCTCAGAGGATGAG+AGG 0.537381 tig0021758:+11967 Msa1393700:CDS
ATTTCTCTTATAAATAGGAC+CGG 0.540197 tig0021758:+12410 Msa1393700:three_prime_UTR
CTCTTATAAATAGGACCAGA+GGG 0.542613 tig0021758:-12314 None:intergenic
AGTGTCGGAGATATTAAGGT+TGG 0.549755 tig0021758:-10204 None:intergenic
CTCTTATAAATAGGACCGGA+GGG 0.554392 tig0021758:+12414 Msa1393700:three_prime_UTR
ATCAACTGGTTCAGGAGTGT+CGG 0.554412 tig0021758:-10219 None:intergenic
TGTTGAGGATTAAGGTGGTG+TGG 0.555356 tig0021758:-10134 None:intergenic
AACAGTTCTCGTGTACATCA+TGG 0.561270 tig0021758:-12115 None:intergenic
GTTGAGGATTAAGGTGGTGT+GGG 0.570563 tig0021758:-10133 None:intergenic
TGGAAATGAAAAGTGGGAGA+AGG 0.570598 tig0021758:-10106 None:intergenic
ATTCCCATTCTCCTCAAGGA+TGG 0.579989 tig0021758:-11053 None:intergenic
GGAGATGTTGAGGATTAAGG+TGG 0.587846 tig0021758:-10139 None:intergenic
TGCAGGTTATTCCATCCTTG+AGG 0.587883 tig0021758:+11042 Msa1393700:intron
ACTCAATATTATCATCCTTG+AGG 0.593636 tig0021758:-12228 None:intergenic
GGGTTAGTGGAAATGAAAAG+TGG 0.602812 tig0021758:-10113 None:intergenic
GACAGATTCAAGAATCCTCA+AGG 0.611144 tig0021758:+12213 Msa1393700:CDS
TCTCTTATAAATAGGACCGG+AGG 0.614852 tig0021758:+12413 Msa1393700:three_prime_UTR
TGTGTATATTCAACTGAGCA+GGG 0.620117 tig0021758:+12470 Msa1393700:three_prime_UTR
GAAATGAAAAGTGGGAGAAG+GGG 0.622002 tig0021758:-10104 None:intergenic
CATAGCAGCCCTTTGCTCAG+AGG 0.625766 tig0021758:+11959 Msa1393700:CDS
TCTCTTATAAATAGGACCAG+AGG 0.626066 tig0021758:-12315 None:intergenic
AGTGGTTGCAAATGGAGCAG+AGG 0.626606 tig0021758:-10166 None:intergenic
TTTCGAAGCGAAAACTTCAG+TGG 0.633639 tig0021758:-10003 None:intergenic
TTCTTGAATCTGTCAATCAG+AGG 0.641603 tig0021758:-12204 None:intergenic
CCATCAACATCAATGTGGTG+AGG 0.651681 tig0021758:-10302 None:intergenic
GGTTAGTGGAAATGAAAAGT+GGG 0.652172 tig0021758:-10112 None:intergenic
TGGGGAATCTGTGAAAAGAG+TGG 0.656361 tig0021758:-10184 None:intergenic
TTGAATCTGTCAATCAGAGG+CGG 0.671155 tig0021758:-12201 None:intergenic
TGATTGTTAGAAAAGTTGTG+AGG 0.694957 tig0021758:+11084 Msa1393700:CDS
TTAAACCATCAACATCAATG+TGG 0.739906 tig0021758:-10307 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAATGTATAAATAA+AGG + tig0021758:11493-11512 Msa1393700:intron 10.0%
!! AAGAAAAATGTATAAATAAA+GGG + tig0021758:11494-11513 Msa1393700:intron 10.0%
!!! TTTTTTCCTTTTTCAATATT+TGG + tig0021758:10947-10966 Msa1393700:intron 15.0%
!! AGAAAAATGTATAAATAAAG+GGG + tig0021758:11495-11514 Msa1393700:intron 15.0%
!! TAATTAGAGTTATATTGTAT+TGG + tig0021758:11558-11577 Msa1393700:intron 15.0%
!! AATTAGAGTTATATTGTATT+GGG + tig0021758:11559-11578 Msa1393700:intron 15.0%
!! GAAATCAATGGAATTAAAAT+GGG + tig0021758:10022-10041 Msa1393700:CDS 20.0%
!!! TCTTTGTTTTGAAAAAATGT+AGG - tig0021758:10470-10489 None:intergenic 20.0%
!! CAAAATAACCAAAATAATGA+TGG - tig0021758:10559-10578 None:intergenic 20.0%
!!! TATTTTGGTTATTTTGTTTC+TGG + tig0021758:10563-10582 Msa1393700:intron 20.0%
!!! ATTTTGGTTATTTTGTTTCT+GGG + tig0021758:10564-10583 Msa1393700:intron 20.0%
!! ATTGTCAGTTATTGTAATTA+TGG - tig0021758:10624-10643 None:intergenic 20.0%
!! AAAGTGGAATATATTGTATT+GGG + tig0021758:11518-11537 Msa1393700:intron 20.0%
!! AGTTGTATTGAAAATTGAAA+TGG + tig0021758:11591-11610 Msa1393700:intron 20.0%
!!! GACACTTTTTTTTAGAAAAT+GGG + tig0021758:11628-11647 Msa1393700:intron 20.0%
!! CCAAAATTTCTCTTATAAAT+AGG - tig0021758:12326-12345 None:intergenic 20.0%
!!! CCTATTTATAAGAGAAATTT+TGG + tig0021758:12323-12342 Msa1393700:three_prime_UTR 20.0%
!! ATTGTATCTGGTTAAAAAAT+TGG + tig0021758:12358-12377 Msa1393700:three_prime_UTR 20.0%
!! CCTATTTATAAGAGAAATTT+GGG - tig0021758:12323-12342 None:intergenic 20.0%
!! TCCTATTTATAAGAGAAATT+TGG - tig0021758:12322-12341 None:intergenic 20.0%
! CGAAATCAATGGAATTAAAA+TGG + tig0021758:10021-10040 Msa1393700:CDS 25.0%
! ATGAGTTCAATTTCATATCT+TGG + tig0021758:10526-10545 Msa1393700:intron 25.0%
!!! CTATAAGCTGTTTTCTATTT+TGG - tig0021758:10827-10846 None:intergenic 25.0%
! AATAAGTTGTTTATCCAAAC+AGG + tig0021758:10880-10899 Msa1393700:intron 25.0%
! ATAAGTTGTTTATCCAAACA+GGG + tig0021758:10881-10900 Msa1393700:intron 25.0%
!! ACATGTTTTTCATTAACAAC+AGG - tig0021758:10923-10942 None:intergenic 25.0%
! TCAATGCCAAATATTGAAAA+AGG - tig0021758:10956-10975 None:intergenic 25.0%
!! ATCATTCATGAAGTTTTTGT+TGG + tig0021758:11223-11242 Msa1393700:intron 25.0%
! TGAATTTAATCACAATGTGT+CGG + tig0021758:11376-11395 Msa1393700:intron 25.0%
! GAAAGTGGAATATATTGTAT+TGG + tig0021758:11517-11536 Msa1393700:intron 25.0%
!! TTGAAATGGCTCAATTATTT+TGG + tig0021758:11605-11624 Msa1393700:intron 25.0%
!! TGAAATGGCTCAATTATTTT+GGG + tig0021758:11606-11625 Msa1393700:intron 25.0%
!!! GGACACTTTTTTTTAGAAAA+TGG + tig0021758:11627-11646 Msa1393700:intron 25.0%
! TAAGTCAAATTAAACCTTGT+AGG + tig0021758:11774-11793 Msa1393700:intron 25.0%
! AATATAAAGGGTTGTGTATA+AGG + tig0021758:11840-11859 Msa1393700:intron 25.0%
! ATATAAAGGGTTGTGTATAA+GGG + tig0021758:11841-11860 Msa1393700:intron 25.0%
! AAGTTCCAAATATCTGAATA+TGG + tig0021758:11897-11916 Msa1393700:intron 25.0%
! ATAGACCATATTCAGATATT+TGG - tig0021758:11905-11924 None:intergenic 25.0%
! TTGTTCATTATTCCTATACA+TGG + tig0021758:11990-12009 Msa1393700:intron 25.0%
!!! TTTTCTCAGGTTGTTTTTTA+AGG + tig0021758:12056-12075 Msa1393700:intron 25.0%
!!! AAAATTGTTGTTTAAGCAAC+CGG + tig0021758:12280-12299 Msa1393700:three_prime_UTR 25.0%
! TCAACAAAAGTTATTGTATC+TGG + tig0021758:12346-12365 Msa1393700:three_prime_UTR 25.0%
! CCCAAATTTCTCTTATAAAT+AGG + tig0021758:12405-12424 Msa1393700:three_prime_UTR 25.0%
! ATTTCTCTTATAAATAGGAC+CGG + tig0021758:12410-12429 Msa1393700:three_prime_UTR 25.0%
!!! TTACCATTTTTTGTCATTCT+TGG + tig0021758:12445-12464 Msa1393700:three_prime_UTR 25.0%
!! TTTTTGTTTGTGTGTGGTTT+TGG - tig0021758:10072-10091 None:intergenic 30.0%
TTAAACCATCAACATCAATG+TGG - tig0021758:10310-10329 None:intergenic 30.0%
TGCTTCTATATACGATGTTA+TGG + tig0021758:10430-10449 Msa1393700:CDS 30.0%
!!! GTTTTGAAAAAATGTAGGCA+AGG - tig0021758:10465-10484 None:intergenic 30.0%
! CAATTTCATATCTTGGTTCT+TGG + tig0021758:10533-10552 Msa1393700:intron 30.0%
! TTTCTGGGTTTTCTTAGTTT+TGG + tig0021758:10579-10598 Msa1393700:intron 30.0%
!! TTCTGGGTTTTCTTAGTTTT+GGG + tig0021758:10580-10599 Msa1393700:intron 30.0%
ACTAACTATTTATGCCTGTT+TGG + tig0021758:10683-10702 Msa1393700:intron 30.0%
TCAAATAAGTCAATCCAAAC+AGG - tig0021758:10700-10719 None:intergenic 30.0%
AAACAGTCTCACAATACTTA+TGG - tig0021758:10735-10754 None:intergenic 30.0%
!! TAAGTATTGTGAGACTGTTT+TGG + tig0021758:10735-10754 Msa1393700:intron 30.0%
CAGCTTATAGCTTATACAAT+AGG + tig0021758:10837-10856 Msa1393700:intron 30.0%
TATAAGATATAAGCCCTGTT+TGG - tig0021758:10897-10916 None:intergenic 30.0%
!! CAATATTTGGCATTGATTTG+AGG + tig0021758:10960-10979 Msa1393700:intron 30.0%
TGATTGTTAGAAAAGTTGTG+AGG + tig0021758:11084-11103 Msa1393700:CDS 30.0%
ATTGAATCATCACAGAAGAT+AGG - tig0021758:11127-11146 None:intergenic 30.0%
ATTAGATTCTCATCAGCAAA+AGG - tig0021758:11203-11222 None:intergenic 30.0%
! TTTTTGTTGGCATAGAGTTT+TGG + tig0021758:11236-11255 Msa1393700:intron 30.0%
TGTATAAATAAAGGGGAAAG+TGG + tig0021758:11502-11521 Msa1393700:intron 30.0%
TGCAATAAAAGCTGTCATAT+TGG + tig0021758:11721-11740 Msa1393700:intron 30.0%
GCAATAAAAGCTGTCATATT+GGG + tig0021758:11722-11741 Msa1393700:intron 30.0%
CTTGTAGGAGTTGAATATAA+GGG + tig0021758:11789-11808 Msa1393700:intron 30.0%
TTGTAGGAGTTGAATATAAG+GGG + tig0021758:11790-11809 Msa1393700:intron 30.0%
ATTGATCTTTAACCAGAGAT+GGG - tig0021758:11872-11891 None:intergenic 30.0%
TATTGATCTTTAACCAGAGA+TGG - tig0021758:11873-11892 None:intergenic 30.0%
GATAAAACTAGACCATGTAT+AGG - tig0021758:12005-12024 None:intergenic 30.0%
CATCAATTACTGTTCACAAA+GGG - tig0021758:12027-12046 None:intergenic 30.0%
ACATCAATTACTGTTCACAA+AGG - tig0021758:12028-12047 None:intergenic 30.0%
!!! TTTTTTAAGGCTTGTGGATT+TGG + tig0021758:12069-12088 Msa1393700:CDS 30.0%
ACTCAATATTATCATCCTTG+AGG - tig0021758:12231-12250 None:intergenic 30.0%
TCAACAAAAGTGGATGTATT+TGG - tig0021758:12385-12404 None:intergenic 30.0%
AAGCCAAGAATGACAAAAAA+TGG - tig0021758:12451-12470 None:intergenic 30.0%
GTACAGAGTATATCCTAAAT+CGG + tig0021758:12580-12599 Msa1393700:three_prime_UTR 30.0%
!! CATTTAGGATATACCGATTT+AGG - tig0021758:12596-12615 None:intergenic 30.0%
CTGAAATCTTTCTCACATTT+AGG - tig0021758:12611-12630 None:intergenic 30.0%
! AGTTTTCGCTTCGAAATCAA+TGG + tig0021758:10010-10029 Msa1393700:CDS 35.0%
!!! TAGCGTTTTTTGTTTGTGTG+TGG - tig0021758:10078-10097 None:intergenic 35.0%
GGTTAGTGGAAATGAAAAGT+GGG - tig0021758:10115-10134 None:intergenic 35.0%
TTTGGAGATGTTGAGGATTA+AGG - tig0021758:10145-10164 None:intergenic 35.0%
! TTGTTGATATCAACTGGTTC+AGG - tig0021758:10230-10249 None:intergenic 35.0%
!!! CATTGTTGATTTTTTCACGC+CGG + tig0021758:10346-10365 Msa1393700:CDS 35.0%
!! CTGAACGTGATTTGTTGATT+GGG + tig0021758:10375-10394 Msa1393700:CDS 35.0%
! GTTCTTGGCCATCATTATTT+TGG + tig0021758:10548-10567 Msa1393700:intron 35.0%
TTCTGTGATGATTCAATGCA+TGG + tig0021758:11130-11149 Msa1393700:intron 35.0%
! TGAAAGTGAGTATCTAGTTC+AGG - tig0021758:11158-11177 None:intergenic 35.0%
AATCTGTGTGAAAACCAAAC+TGG - tig0021758:11474-11493 None:intergenic 35.0%
AGAAAATGGGTCAGATATTC+CGG + tig0021758:11641-11660 Msa1393700:intron 35.0%
CCTTATATTCAACTCCTACA+AGG - tig0021758:11791-11810 None:intergenic 35.0%
CCTTGTAGGAGTTGAATATA+AGG + tig0021758:11788-11807 Msa1393700:intron 35.0%
!!! AGGTTGTTTTTTAAGGCTTG+TGG + tig0021758:12063-12082 Msa1393700:intron 35.0%
AACTGTTTCATCAACAATCC+AGG + tig0021758:12131-12150 Msa1393700:CDS 35.0%
TTCTTGAATCTGTCAATCAG+AGG - tig0021758:12207-12226 None:intergenic 35.0%
CTCTTATAAATAGGACCAGA+GGG - tig0021758:12317-12336 None:intergenic 35.0%
TCTCTTATAAATAGGACCAG+AGG - tig0021758:12318-12337 None:intergenic 35.0%
GAAATTTGGGTCAACAAAAG+TGG - tig0021758:12395-12414 None:intergenic 35.0%
TTGTGTATATTCAACTGAGC+AGG + tig0021758:12469-12488 Msa1393700:three_prime_UTR 35.0%
TGTGTATATTCAACTGAGCA+GGG + tig0021758:12470-12489 Msa1393700:three_prime_UTR 35.0%
! TCGTTGGTTGCAGACAAAAA+TGG + tig0021758:9959-9978 Msa1393700:exon 40.0%
TTGGTTGCAGACAAAAATGG+CGG + tig0021758:9962-9981 Msa1393700:exon 40.0%
TTTCGAAGCGAAAACTTCAG+TGG - tig0021758:10006-10025 None:intergenic 40.0%
GAAATGAAAAGTGGGAGAAG+GGG - tig0021758:10107-10126 None:intergenic 40.0%
GGAAATGAAAAGTGGGAGAA+GGG - tig0021758:10108-10127 None:intergenic 40.0%
TGGAAATGAAAAGTGGGAGA+AGG - tig0021758:10109-10128 None:intergenic 40.0%
! GGGTTAGTGGAAATGAAAAG+TGG - tig0021758:10116-10135 None:intergenic 40.0%
GTGAAAAGAGTGGTTGCAAA+TGG - tig0021758:10177-10196 None:intergenic 40.0%
TGTCGGAGATATTAAGGTTG+GGG - tig0021758:10205-10224 None:intergenic 40.0%
GTGTCGGAGATATTAAGGTT+GGG - tig0021758:10206-10225 None:intergenic 40.0%
! AGTGTCGGAGATATTAAGGT+TGG - tig0021758:10207-10226 None:intergenic 40.0%
!! CGAAGCTTGTTGATATCAAC+TGG - tig0021758:10236-10255 None:intergenic 40.0%
CGGCGTGAAAAAATCAACAA+TGG - tig0021758:10348-10367 None:intergenic 40.0%
AAATCACGTTCAGGAGAAAC+CGG - tig0021758:10368-10387 None:intergenic 40.0%
CCAATCAACAAATCACGTTC+AGG - tig0021758:10377-10396 None:intergenic 40.0%
!! CCTGAACGTGATTTGTTGAT+TGG + tig0021758:10374-10393 Msa1393700:CDS 40.0%
!! CGTGATTTGTTGATTGGGTT+TGG + tig0021758:10380-10399 Msa1393700:CDS 40.0%
! TTGCGTTTTCCCATAGTTGA+CGG + tig0021758:10993-11012 Msa1393700:intron 40.0%
GAATAATGCCCGTCAACTAT+GGG - tig0021758:11005-11024 None:intergenic 40.0%
TGAATAATGCCCGTCAACTA+TGG - tig0021758:11006-11025 None:intergenic 40.0%
TATTCCATCCTTGAGGAGAA+TGG + tig0021758:11049-11068 Msa1393700:CDS 40.0%
ATTCCATCCTTGAGGAGAAT+GGG + tig0021758:11050-11069 Msa1393700:CDS 40.0%
CATCACAGAAGATAGGCAAA+AGG - tig0021758:11120-11139 None:intergenic 40.0%
! TCTACTATGAGGCACTAACA+CGG + tig0021758:11297-11316 Msa1393700:intron 40.0%
GTCGATGTGTAAGTGTGTAT+TGG - tig0021758:11326-11345 None:intergenic 40.0%
GAAAATGGGTCAGATATTCC+GGG + tig0021758:11642-11661 Msa1393700:intron 40.0%
!! GATGTTCTCGACTTTTTCTC+AGG + tig0021758:12043-12062 Msa1393700:intron 40.0%
! TTTAAGGCTTGTGGATTTGG+TGG + tig0021758:12072-12091 Msa1393700:CDS 40.0%
!!! TGGATTTGGTGGTGACATTT+TGG + tig0021758:12083-12102 Msa1393700:CDS 40.0%
AACAGTTCTCGTGTACATCA+TGG - tig0021758:12118-12137 None:intergenic 40.0%
TTGAATCTGTCAATCAGAGG+CGG - tig0021758:12204-12223 None:intergenic 40.0%
GACAGATTCAAGAATCCTCA+AGG + tig0021758:12213-12232 Msa1393700:CDS 40.0%
TCTCTTATAAATAGGACCGG+AGG + tig0021758:12413-12432 Msa1393700:three_prime_UTR 40.0%
CTCTTATAAATAGGACCGGA+GGG + tig0021758:12414-12433 Msa1393700:three_prime_UTR 40.0%
ATGGTAACATTTACTCCCTC+CGG - tig0021758:12432-12451 None:intergenic 40.0%
!! ATTAAGGTGGTGTGGGTTAG+TGG - tig0021758:10129-10148 None:intergenic 45.0%
GTTGAGGATTAAGGTGGTGT+GGG - tig0021758:10136-10155 None:intergenic 45.0%
TGTTGAGGATTAAGGTGGTG+TGG - tig0021758:10137-10156 None:intergenic 45.0%
GGAGATGTTGAGGATTAAGG+TGG - tig0021758:10142-10161 None:intergenic 45.0%
!!! CAGAGGTTTTGGAGATGTTG+AGG - tig0021758:10152-10171 None:intergenic 45.0%
! TGCAAATGGAGCAGAGGTTT+TGG - tig0021758:10163-10182 None:intergenic 45.0%
TGGGGAATCTGTGAAAAGAG+TGG - tig0021758:10187-10206 None:intergenic 45.0%
!! CAGGAGTGTCGGAGATATTA+AGG - tig0021758:10211-10230 None:intergenic 45.0%
! ATCAACTGGTTCAGGAGTGT+CGG - tig0021758:10222-10241 None:intergenic 45.0%
CCATCAACATCAATGTGGTG+AGG - tig0021758:10305-10324 None:intergenic 45.0%
CCTCACCACATTGATGTTGA+TGG + tig0021758:10302-10321 Msa1393700:CDS 45.0%
!!! GGTCGTGCTGTTTCTGATTT+TGG + tig0021758:10401-10420 Msa1393700:CDS 45.0%
! TGCGTTTTCCCATAGTTGAC+GGG + tig0021758:10994-11013 Msa1393700:intron 45.0%
AACATGCTTCAGTGTCTTGC+AGG + tig0021758:11025-11044 Msa1393700:intron 45.0%
TGCAGGTTATTCCATCCTTG+AGG + tig0021758:11042-11061 Msa1393700:intron 45.0%
ATTCCCATTCTCCTCAAGGA+TGG - tig0021758:11056-11075 None:intergenic 45.0%
TCCTTGAGGAGAATGGGAAT+TGG + tig0021758:11056-11075 Msa1393700:CDS 45.0%
TCCAATTCCCATTCTCCTCA+AGG - tig0021758:11060-11079 None:intergenic 45.0%
TCACGTCAGTCTCTACTATG+AGG + tig0021758:11286-11305 Msa1393700:intron 45.0%
! ACGCCATCAATTAGAAGCGT+TGG + tig0021758:11414-11433 Msa1393700:intron 45.0%
GCACCAACGCTTCTAATTGA+TGG - tig0021758:11420-11439 None:intergenic 45.0%
!! CAAACTGGCTCGGATAAAAC+GGG - tig0021758:11459-11478 None:intergenic 45.0%
!! CCAAACTGGCTCGGATAAAA+CGG - tig0021758:11460-11479 None:intergenic 45.0%
GTGTGAAAACCAAACTGGCT+CGG - tig0021758:11469-11488 None:intergenic 45.0%
ATAAGGGTTCTTCCCATCTC+TGG + tig0021758:11857-11876 Msa1393700:intron 45.0%
GCGGAACCAACAATAGCTTT+CGG - tig0021758:12185-12204 None:intergenic 45.0%
GTTGTTTAAGCAACCGGAAG+AGG + tig0021758:12286-12305 Msa1393700:three_prime_UTR 45.0%
!!! ATTTGTTTTTTTTTTTAAAT+TGG - tig0021758:11823-11842 None:intergenic 5.0%
!! AAAAAAAAAAACAAATATAA+AGG + tig0021758:11827-11846 Msa1393700:intron 5.0%
!! AAAAAAAAAACAAATATAAA+GGG + tig0021758:11828-11847 Msa1393700:intron 5.0%
CAGACAAAAATGGCGGCAAC+AGG + tig0021758:9969-9988 Msa1393700:exon 50.0%
AGTGGTTGCAAATGGAGCAG+AGG - tig0021758:10169-10188 None:intergenic 50.0%
GTAGAGACTGACGTGAGTGA+GGG - tig0021758:11282-11301 None:intergenic 50.0%
AGTAGAGACTGACGTGAGTG+AGG - tig0021758:11283-11302 None:intergenic 50.0%
! CCGTTTTATCCGAGCCAGTT+TGG + tig0021758:11457-11476 Msa1393700:intron 50.0%
ATGGGTCAGATATTCCGGGA+CGG + tig0021758:11646-11665 Msa1393700:intron 50.0%
CCTCTCATCCTCTGAGCAAA+GGG - tig0021758:11970-11989 None:intergenic 50.0%
ACCTCTCATCCTCTGAGCAA+AGG - tig0021758:11971-11990 None:intergenic 50.0%
! TGACGGTTTGCTCACTTTCC+TGG - tig0021758:12152-12171 None:intergenic 50.0%
AACAATAGCTTTCGGCCTGC+AGG - tig0021758:12177-12196 None:intergenic 50.0%
TGCAGGCCGAAAGCTATTGT+TGG + tig0021758:12176-12195 Msa1393700:CDS 50.0%
CTCGGATAAAACGGGACGGA+GGG - tig0021758:11451-11470 None:intergenic 55.0%
!! CTGGCTCGGATAAAACGGGA+CGG - tig0021758:11455-11474 None:intergenic 55.0%
GTCAGATATTCCGGGACGGA+GGG + tig0021758:11650-11669 Msa1393700:intron 55.0%
GACGGAGGGAGTAGTTCTCT+AGG + tig0021758:11664-11683 Msa1393700:intron 55.0%
CATAGCAGCCCTTTGCTCAG+AGG + tig0021758:11959-11978 Msa1393700:CDS 55.0%
CCCTTTGCTCAGAGGATGAG+AGG + tig0021758:11967-11986 Msa1393700:CDS 55.0%
CCAGAGGGAGTAACCTCTTC+CGG - tig0021758:12302-12321 None:intergenic 55.0%
GCTCGGATAAAACGGGACGG+AGG - tig0021758:11452-11471 None:intergenic 60.0%
GGTCAGATATTCCGGGACGG+AGG + tig0021758:11649-11668 Msa1393700:intron 60.0%
GAGAACTACTCCCTCCGTCC+CGG - tig0021758:11663-11682 None:intergenic 60.0%
GAGCAAACCGTCACACCTGC+AGG + tig0021758:12159-12178 Msa1393700:CDS 60.0%
CTTTCGGCCTGCAGGTGTGA+CGG - tig0021758:12169-12188 None:intergenic 60.0%
CCGGAAGAGGTTACTCCCTC+TGG + tig0021758:12299-12318 Msa1393700:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0021758 gene 9953 12640 9953 ID=Msa1393700;Name=Msa1393700
tig0021758 mRNA 9953 12640 9953 ID=Msa1393700-mRNA-1;Parent=Msa1393700;Name=Msa1393700-mRNA-1;_AED=0.25;_eAED=0.25;_QI=25|1|1|1|1|1|4|401|254
tig0021758 exon 9953 10451 9953 ID=Msa1393700-mRNA-1:exon:13971;Parent=Msa1393700-mRNA-1
tig0021758 exon 11047 11105 11047 ID=Msa1393700-mRNA-1:exon:13972;Parent=Msa1393700-mRNA-1
tig0021758 exon 11932 11988 11932 ID=Msa1393700-mRNA-1:exon:13973;Parent=Msa1393700-mRNA-1
tig0021758 exon 12065 12640 12065 ID=Msa1393700-mRNA-1:exon:13974;Parent=Msa1393700-mRNA-1
tig0021758 five_prime_UTR 9953 9977 9953 ID=Msa1393700-mRNA-1:five_prime_utr;Parent=Msa1393700-mRNA-1
tig0021758 CDS 9978 10451 9978 ID=Msa1393700-mRNA-1:cds;Parent=Msa1393700-mRNA-1
tig0021758 CDS 11047 11105 11047 ID=Msa1393700-mRNA-1:cds;Parent=Msa1393700-mRNA-1
tig0021758 CDS 11932 11988 11932 ID=Msa1393700-mRNA-1:cds;Parent=Msa1393700-mRNA-1
tig0021758 CDS 12065 12239 12065 ID=Msa1393700-mRNA-1:cds;Parent=Msa1393700-mRNA-1
tig0021758 three_prime_UTR 12240 12640 12240 ID=Msa1393700-mRNA-1:three_prime_utr;Parent=Msa1393700-mRNA-1
Gene Sequence

>Msa1393700

ATGGCGGCAACAGGAGTTGCAGCAGCCACTGAAGTTTTCGCTTCGAAATCAATGGAATTAAAATGGGTAAGAACAAGAACAAGAAGAATCACCAAAACCACACACAAACAAAAAACGCTATCAACACCCCTTCTCCCACTTTTCATTTCCACTAACCCACACCACCTTAATCCTCAACATCTCCAAAACCTCTGCTCCATTTGCAACCACTCTTTTCACAGATTCCCCAACCTTAATATCTCCGACACTCCTGAACCAGTTGATATCAACAAGCTTCGCATTGCTCTTTCTCATAGTGACGTTCTTGTCTCTGTCTTCTGTAAACCTCACCACATTGATGTTGATGGTTTAACTGAATCTTCATCTTCCATTGTTGATTTTTTCACGCCGGTTTCTCCTGAACGTGATTTGTTGATTGGGTTTGGTCGTGCTGTTTCTGATTTTGGTCTCACTGCTTCTATATACGATGTTATGGTTATTCCATCCTTGAGGAGAATGGGAATTGGAAAAATGATTGTTAGAAAAGTTGTGAGAATGCTTACAAATAGAGACATCTATGACATAGCAGCCCTTTGCTCAGAGGATGAGAGGTTGTTTTTTAAGGCTTGTGGATTTGGTGGTGACATTTTGGACTCTACTACCATGATGTACACGAGAACTGTTTCATCAACAATCCAGGAAAGTGAGCAAACCGTCACACCTGCAGGCCGAAAGCTATTGTTGGTTCCGCCTCTGATTGACAGATTCAAGAATCCTCAAGGATGA
Protein sequence

>Msa1393700

MAATGVAAATEVFASKSMELKWVRTRTRRITKTTHKQKTLSTPLLPLFISTNPHHLNPQHLQNLCSICNHSFHRFPNLNISDTPEPVDINKLRIALSHSDVLVSVFCKPHHIDVDGLTESSSSIVDFFTPVSPERDLLIGFGRAVSDFGLTASIYDVMVIPSLRRMGIGKMIVRKVVRMLTNRDIYDIAALCSEDERLFFKACGFGGDILDSTTMMYTRTVSSTIQESEQTVTPAGRKLLLVPPLIDRFKNPQG*