AlfalfaGEDB | Msa0518260

M. sativa cultivar zhongmu-4 / Msa0518260

KEGG: K05282
GO: GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0045544 GO:0046394 GO:0048367 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080167 GO:0090567 GO:1901576 GO:1901700 GO:1901701
PFAM: PF14226.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518260	AFK38891.1	96.133	181	7	0	12	192	10	190	7.56e-125	363

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518260	sp\|O04706\|GAO1B_WHEAT	42.767	159	88	2	35	192	5	161	2.69e-38	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518260	I3SF49	96.133	181	7	0	12	192	10	190	3.61e-125	363

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0378210	Msa0518260	0.859131	5.113884e-63	-8.615850e-47
Msa0378220	Msa0518260	0.947124	1.235823e-105	-8.615850e-47
Msa0427430	Msa0518260	0.852588	4.182446e-61	-8.615850e-47
Msa0427440	Msa0518260	0.931585	3.038611e-94	-8.615850e-47
Msa0427490	Msa0518260	0.857909	1.183725e-62	-8.615850e-47
Msa0427500	Msa0518260	0.945347	3.618933e-104	-8.615850e-47
Msa0518260	Msa0518270	0.870151	1.812145e-66	-8.615850e-47
Msa0518260	Msa1345510	0.838780	2.346803e-57	-8.615850e-47
Msa0518260	Msa1345520	0.933035	3.461394e-95	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518260	MtrunA17_Chr3g0130391	96.133	181	7	0	12	192	10	190	2.10e-127	363
Msa0518260	MtrunA17_Chr1g0191201	53.571	168	75	2	27	192	11	177	1.92e-56	182
Msa0518260	MtrunA17_Chr8g0384481	48.901	182	87	3	11	192	4	179	1.19e-52	172
Msa0518260	MtrunA17_Chr3g0125421	54.605	152	67	2	41	192	10	159	1.73e-51	169
Msa0518260	MtrunA17_Chr8g0384461	50.602	166	78	2	28	192	6	168	2.79e-50	166
Msa0518260	MtrunA17_Chr1g0204181	42.405	158	91	0	35	192	26	183	2.70e-41	143
Msa0518260	MtrunA17_Chr6g0474391	39.227	181	110	0	12	192	8	188	1.74e-40	141
Msa0518260	MtrunA17_Chr8g0351101	41.808	177	100	3	17	192	3	177	4.84e-38	134
Msa0518260	MtrunA17_Chr7g0239101	28.947	152	77	5	27	166	18	150	8.68e-13	65.9
Msa0518260	MtrunA17_Chr8g0375661	31.148	122	82	1	46	165	28	149	1.61e-12	62.8
Msa0518260	MtrunA17_Chr8g0375641	29.508	122	84	1	46	165	28	149	2.75e-12	62.4
Msa0518260	MtrunA17_Chr8g0375671	29.508	122	84	1	46	165	28	149	7.86e-12	63.2
Msa0518260	MtrunA17_Chr2g0332191	31.818	132	75	3	37	165	21	140	8.28e-12	62.8
Msa0518260	MtrunA17_Chr5g0423191	29.268	123	81	3	42	162	19	137	1.30e-11	62.4
Msa0518260	MtrunA17_Chr5g0398291	31.746	126	77	3	46	162	18	143	1.33e-11	62.4
Msa0518260	MtrunA17_Chr7g0233671	31.579	114	76	1	64	175	37	150	1.37e-11	60.1
Msa0518260	MtrunA17_Chr7g0239121	29.252	147	89	5	27	169	14	149	3.30e-11	61.2
Msa0518260	MtrunA17_Chr5g0423251	30.894	123	79	3	42	162	19	137	5.84e-11	60.5
Msa0518260	MtrunA17_Chr5g0421041	27.273	121	84	1	42	162	19	135	8.44e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0518260	AT5G07200.1	40.761	184	106	3	11	192	1	183	2.94e-38	135
Msa0518260	AT5G51810.2	38.150	173	100	2	20	192	17	182	2.33e-36	128
Msa0518260	AT5G51810.1	38.150	173	100	2	20	192	17	182	1.25e-35	128
Msa0518260	AT4G25420.2	40.506	158	93	1	35	192	28	184	3.88e-35	125
Msa0518260	AT4G25420.1	40.506	158	93	1	35	192	28	184	1.76e-34	125
Msa0518260	AT1G60980.1	38.323	167	99	3	30	192	18	184	2.46e-31	117
Msa0518260	AT1G44090.1	37.209	172	104	3	22	192	17	185	2.72e-30	114
Msa0518260	AT1G80340.1	34.375	128	73	3	46	171	26	144	1.69e-14	70.9
Msa0518260	AT4G16770.1	36.607	112	59	4	57	164	11	114	1.56e-11	62.0

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAGAATTGGAATCATTT+TGG	0.198738	3_4:-70114207	None:intergenic
GTTGAAGTACTTAGTTCATT+AGG	0.274284	3_4:-70113725	None:intergenic
GCTGATAGGTAGCTTGAAAA+TGG	0.275391	3_4:-70114068	None:intergenic
GATGGCCAAATGAACTCTTT+TGG	0.282606	3_4:-70113845	None:intergenic
AGAGTTCATTTGGCCATCTA+TGG	0.293217	3_4:+70113850	Msa0518260:CDS
GTAGTTAACAATCTGAGAAT+TGG	0.352416	3_4:-70114218	None:intergenic
ACATTCCAAAAGAGTTCATT+TGG	0.379504	3_4:+70113840	Msa0518260:CDS
TGTCTCTTTCCATGGTAACT+TGG	0.412911	3_4:-70114164	None:intergenic
TTCTTCCAAGTGATTAATCA+TGG	0.415834	3_4:+70114001	Msa0518260:CDS
AGAGTGCATGGTGGTGTCTC+TGG	0.415871	3_4:+70114109	Msa0518260:CDS
GTGTTAACTAAATCCATAGA+TGG	0.436495	3_4:-70113863	None:intergenic
GCACTTAGCTTCTTGCTGAT+AGG	0.441284	3_4:-70114082	None:intergenic
CTGATAGGTAGCTTGAAAAT+GGG	0.444807	3_4:-70114067	None:intergenic
AACTTTCAACAGACAGGGTA+AGG	0.463250	3_4:+70114262	Msa0518260:CDS
GGAGAAAACTTTCAACAGAC+AGG	0.468311	3_4:+70114256	Msa0518260:CDS
CCACACCATGATTAATCACT+TGG	0.471767	3_4:-70114006	None:intergenic
GGTGGTGTCTCTGGCTACTC+AGG	0.473480	3_4:+70114118	Msa0518260:CDS
TGGTACATGCAAAATGCAAA+TGG	0.478696	3_4:+70113698	None:intergenic
ATGCATCATTGATGAGCTCT+TGG	0.485155	3_4:-70114030	None:intergenic
AAAGAAAATGTCTCTTTCCA+TGG	0.522658	3_4:-70114172	None:intergenic
GACTTAAGTGTCATGAAAAG+TGG	0.534486	3_4:+70113917	Msa0518260:CDS
CAAGTGCTGCTGAGCTTGTG+AGG	0.575589	3_4:+70113957	Msa0518260:CDS
CTAAGTGCTAAGAGAGTGCA+TGG	0.578017	3_4:+70114097	Msa0518260:CDS
GATATTCTTCCAAGTTACCA+TGG	0.583162	3_4:+70114155	Msa0518260:CDS
ATGACACTTAAGTCTATGAG+AGG	0.591503	3_4:-70113908	None:intergenic
AGGAAAGCATGTTTGAAACA+TGG	0.621611	3_4:+70113977	Msa0518260:CDS
GAGAAAACTTTCAACAGACA+GGG	0.632132	3_4:+70114257	Msa0518260:CDS
TGATAGGTAGCTTGAAAATG+GGG	0.637100	3_4:-70114066	None:intergenic
TTTAGTTAACACAGCCCAAG+AGG	0.637386	3_4:+70113874	Msa0518260:CDS
GAAACCCAAAACAGAAAACA+TGG	0.640924	3_4:+70113778	Msa0518260:CDS
CCAAGTGATTAATCATGGTG+TGG	0.641363	3_4:+70114006	Msa0518260:CDS
AGTGCTAAGAGAGTGCATGG+TGG	0.823438	3_4:+70114100	Msa0518260:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTGAAGTACTTAGTTCATT+AGG	-	chr3_4:70113728-70113747	None:intergenic	30.0%
!!!	GTTTCTCTGTTTTTAATGCA+GGG	-	chr3_4:70113763-70113782	None:intergenic	30.0%
!	GAAACCATGTTTTCTGTTTT+GGG	-	chr3_4:70113785-70113804	None:intergenic	30.0%
!	AGAAACCATGTTTTCTGTTT+TGG	-	chr3_4:70113786-70113805	None:intergenic	30.0%
	ACATTCCAAAAGAGTTCATT+TGG	+	chr3_4:70113840-70113859	Msa0518260:CDS	30.0%
	GTGTTAACTAAATCCATAGA+TGG	-	chr3_4:70113866-70113885	None:intergenic	30.0%
	TTCTTCCAAGTGATTAATCA+TGG	+	chr3_4:70114001-70114020	Msa0518260:CDS	30.0%
	AAAGAAAATGTCTCTTTCCA+TGG	-	chr3_4:70114175-70114194	None:intergenic	30.0%
!	TCTGAGAATTGGAATCATTT+TGG	-	chr3_4:70114210-70114229	None:intergenic	30.0%
	GTAGTTAACAATCTGAGAAT+TGG	-	chr3_4:70114221-70114240	None:intergenic	30.0%
!	AACTACTTCAAGTCAGTTTT+AGG	+	chr3_4:70114235-70114254	Msa0518260:CDS	30.0%
!!!	GGTTTCTCTGTTTTTAATGC+AGG	-	chr3_4:70113764-70113783	None:intergenic	35.0%
	GAAACCCAAAACAGAAAACA+TGG	+	chr3_4:70113778-70113797	Msa0518260:CDS	35.0%
	ATGACACTTAAGTCTATGAG+AGG	-	chr3_4:70113911-70113930	None:intergenic	35.0%
	GACTTAAGTGTCATGAAAAG+TGG	+	chr3_4:70113917-70113936	Msa0518260:CDS	35.0%
!	AGGAAAGCATGTTTGAAACA+TGG	+	chr3_4:70113977-70113996	Msa0518260:CDS	35.0%
	TGATAGGTAGCTTGAAAATG+GGG	-	chr3_4:70114069-70114088	None:intergenic	35.0%
	CTGATAGGTAGCTTGAAAAT+GGG	-	chr3_4:70114070-70114089	None:intergenic	35.0%
	GATATTCTTCCAAGTTACCA+TGG	+	chr3_4:70114155-70114174	Msa0518260:CDS	35.0%
	GAGAAAACTTTCAACAGACA+GGG	+	chr3_4:70114257-70114276	Msa0518260:CDS	35.0%
!	GATGGCCAAATGAACTCTTT+TGG	-	chr3_4:70113848-70113867	None:intergenic	40.0%
	AGAGTTCATTTGGCCATCTA+TGG	+	chr3_4:70113850-70113869	Msa0518260:CDS	40.0%
	TTTAGTTAACACAGCCCAAG+AGG	+	chr3_4:70113874-70113893	Msa0518260:CDS	40.0%
!!!	AGGTTCTTTTAGCTCCTCTT+GGG	-	chr3_4:70113891-70113910	None:intergenic	40.0%
	CCACACCATGATTAATCACT+TGG	-	chr3_4:70114009-70114028	None:intergenic	40.0%
	CCAAGTGATTAATCATGGTG+TGG	+	chr3_4:70114006-70114025	Msa0518260:CDS	40.0%
	ATGCATCATTGATGAGCTCT+TGG	-	chr3_4:70114033-70114052	None:intergenic	40.0%
	GCTGATAGGTAGCTTGAAAA+TGG	-	chr3_4:70114071-70114090	None:intergenic	40.0%
	TGTCTCTTTCCATGGTAACT+TGG	-	chr3_4:70114167-70114186	None:intergenic	40.0%
	GGAGAAAACTTTCAACAGAC+AGG	+	chr3_4:70114256-70114275	Msa0518260:CDS	40.0%
!!!	GAGGTTCTTTTAGCTCCTCT+TGG	-	chr3_4:70113892-70113911	None:intergenic	45.0%
	GCACTTAGCTTCTTGCTGAT+AGG	-	chr3_4:70114085-70114104	None:intergenic	45.0%
!	CTAAGTGCTAAGAGAGTGCA+TGG	+	chr3_4:70114097-70114116	Msa0518260:CDS	45.0%
!	AGTGCTAAGAGAGTGCATGG+TGG	+	chr3_4:70114100-70114119	Msa0518260:CDS	50.0%
!	CAAGTGCTGCTGAGCTTGTG+AGG	+	chr3_4:70113957-70113976	Msa0518260:CDS	55.0%
!	AGAGTGCATGGTGGTGTCTC+TGG	+	chr3_4:70114109-70114128	Msa0518260:CDS	55.0%
	GGTGGTGTCTCTGGCTACTC+AGG	+	chr3_4:70114118-70114137	Msa0518260:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	70113704	70114282	70113704	ID=Msa0518260;Name=Msa0518260
chr3_4	mRNA	70113704	70114282	70113704	ID=Msa0518260-mRNA-1;Parent=Msa0518260;Name=Msa0518260-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|192
chr3_4	exon	70113704	70114282	70113704	ID=Msa0518260-mRNA-1:exon:18875;Parent=Msa0518260-mRNA-1
chr3_4	CDS	70113704	70114282	70113704	ID=Msa0518260-mRNA-1:cds;Parent=Msa0518260-mRNA-1

Gene Sequence

>Msa0518260

ATGCAAAATGCAAATGGCTCTCCTAATGAACTAAGTACTTCAACTCTTGTACTTCACCCTGCATTAAAAACAGAGAAACCCAAAACAGAAAACATGGTTTCTATTTTTGACTCAAATTTGCTTCAAAATCAAGTGAACATTCCAAAAGAGTTCATTTGGCCATCTATGGATTTAGTTAACACAGCCCAAGAGGAGCTAAAAGAACCTCTCATAGACTTAAGTGTCATGAAAAGTGGTGATGAAGAAGCTATTGCAAGTGCTGCTGAGCTTGTGAGGAAAGCATGTTTGAAACATGGTTTCTTCCAAGTGATTAATCATGGTGTGGACCAAGAGCTCATCAATGATGCATATTGTGAAGTTGACCCCATTTTCAAGCTACCTATCAGCAAGAAGCTAAGTGCTAAGAGAGTGCATGGTGGTGTCTCTGGCTACTCAGGTGCTCATGCAGATAGATATTCTTCCAAGTTACCATGGAAAGAGACATTTTCTTTTGTATACAATCACCAAAATGATTCCAATTCTCAGATTGTTAACTACTTCAAGTCAGTTTTAGGAGAAAACTTTCAACAGACAGGGTAA

Protein sequence

>Msa0518260

MQNANGSPNELSTSTLVLHPALKTEKPKTENMVSIFDSNLLQNQVNIPKEFIWPSMDLVNTAQEELKEPLIDLSVMKSGDEEAIASAAELVRKACLKHGFFQVINHGVDQELINDAYCEVDPIFKLPISKKLSAKRVHGGVSGYSGAHADRYSSKLPWKETFSFVYNHQNDSNSQIVNYFKSVLGENFQQTG*