AlfalfaGEDB | Msa1345520

M. sativa cultivar zhongmu-4 / Msa1345520

KEGG: K05282
GO: GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0045544 GO:0046394 GO:0048367 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080167 GO:0090567 GO:1901576 GO:1901700 GO:1901701
PFAM: PF14226.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345520	AFK38891.1	96.685	181	6	0	12	192	10	190	1.65e-125	365

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345520	sp\|O04706\|GAO1B_WHEAT	42.138	159	89	2	35	192	5	161	7.82e-38	136

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345520	I3SF49	96.685	181	6	0	12	192	10	190	7.88e-126	365

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1345510	Msa1345520	0.849235	3.684036e-60	-8.615850e-47
Msa0378210	Msa1345520	0.871580	6.135289e-67	-8.615850e-47
Msa0378220	Msa1345520	0.978102	3.975221e-145	-8.615850e-47
Msa0427430	Msa1345520	0.866407	2.916577e-65	-8.615850e-47
Msa0427440	Msa1345520	0.999077	4.906927e-289	-8.615850e-47
Msa0427490	Msa1345520	0.870091	1.895745e-66	-8.615850e-47
Msa0427500	Msa1345520	0.989201	4.098600e-177	-8.615850e-47
Msa0518260	Msa1345520	0.933035	3.461394e-95	-8.615850e-47
Msa0518270	Msa1345520	0.877808	4.667232e-69	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345520	MtrunA17_Chr3g0130391	96.685	181	6	0	12	192	10	190	3.98e-128	365
Msa1345520	MtrunA17_Chr1g0191201	53.571	168	75	2	27	192	11	177	4.12e-56	181
Msa1345520	MtrunA17_Chr8g0384481	48.901	182	87	3	11	192	4	179	2.21e-52	172
Msa1345520	MtrunA17_Chr3g0125421	54.605	152	67	2	41	192	10	159	3.47e-51	168
Msa1345520	MtrunA17_Chr8g0384461	50.602	166	78	2	28	192	6	168	6.16e-50	165
Msa1345520	MtrunA17_Chr1g0204181	42.405	158	91	0	35	192	26	183	2.40e-41	143
Msa1345520	MtrunA17_Chr6g0474391	39.227	181	110	0	12	192	8	188	1.52e-40	141
Msa1345520	MtrunA17_Chr8g0351101	41.243	177	101	3	17	192	3	177	1.45e-37	133
Msa1345520	MtrunA17_Chr7g0239101	34.343	99	60	2	69	166	56	150	1.08e-12	65.5
Msa1345520	MtrunA17_Chr8g0375661	31.148	122	82	1	46	165	28	149	3.49e-12	62.0
Msa1345520	MtrunA17_Chr8g0375641	29.508	122	84	1	46	165	28	149	5.68e-12	61.6
Msa1345520	MtrunA17_Chr2g0332191	31.818	132	75	3	37	165	21	140	7.10e-12	63.2
Msa1345520	MtrunA17_Chr7g0233671	31.579	114	76	1	64	175	37	150	1.31e-11	60.1
Msa1345520	MtrunA17_Chr8g0375671	29.508	122	84	1	46	165	28	149	1.49e-11	62.0
Msa1345520	MtrunA17_Chr5g0423191	29.268	123	81	3	42	162	19	137	2.40e-11	61.6
Msa1345520	MtrunA17_Chr7g0239121	33.333	102	63	2	69	169	52	149	3.33e-11	61.2
Msa1345520	MtrunA17_Chr5g0398291	30.952	126	78	3	46	162	18	143	3.60e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1345520	AT5G07200.1	40.217	184	107	3	11	192	1	183	8.98e-38	134
Msa1345520	AT5G51810.2	37.572	173	101	2	20	192	17	182	6.41e-36	127
Msa1345520	AT5G51810.1	37.572	173	101	2	20	192	17	182	3.77e-35	127
Msa1345520	AT4G25420.2	39.873	158	94	1	35	192	28	184	1.25e-34	124
Msa1345520	AT4G25420.1	39.873	158	94	1	35	192	28	184	5.19e-34	124
Msa1345520	AT1G60980.1	37.725	167	100	3	30	192	18	184	7.56e-31	115
Msa1345520	AT1G44090.1	37.209	172	104	3	22	192	17	185	5.68e-30	113
Msa1345520	AT1G80340.1	34.375	128	73	3	46	171	26	144	1.54e-14	70.9
Msa1345520	AT4G16770.1	36.607	112	59	4	57	164	11	114	1.67e-11	61.6

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAGAATTGGAATCATTT+TGG	0.198738	tig0007212:+12507	Msa1345520:intergenic
GTTGAAGTACTTAGTTCATT+AGG	0.274284	tig0007212:+12989	Msa1345520:intergenic
GCTGATAGGTAGCTTGAAAA+TGG	0.275391	tig0007212:+12646	Msa1345520:intergenic
AGAGTTCATTTGGCCATCTA+TGG	0.293217	tig0007212:-12864	Msa1345520:CDS
GATGGCCAAATGAACTCTTT+TGG	0.339326	tig0007212:+12869	Msa1345520:intergenic
GTAGTTAACAATCTGAGAAT+TGG	0.352416	tig0007212:+12496	Msa1345520:intergenic
ACATGCCAAAAGAGTTCATT+TGG	0.399130	tig0007212:-12874	Msa1345520:CDS
TGTCTCTTTCCATGGTAACT+TGG	0.412911	tig0007212:+12550	Msa1345520:intergenic
TTCTTCCAAGTGATTAATCA+TGG	0.415834	tig0007212:-12713	Msa1345520:CDS
AGAGTGCATGGTGGTGTCTC+TGG	0.415871	tig0007212:-12605	Msa1345520:CDS
GTGTTAACTAAATCCATAGA+TGG	0.436495	tig0007212:+12851	None:intergenic
GCACTTAGCTTCTTGCTGAT+AGG	0.441284	tig0007212:+12632	None:intergenic
CTGATAGGTAGCTTGAAAAT+GGG	0.444807	tig0007212:+12647	None:intergenic
AACTTTCAACAGACAGGGTA+AGG	0.463250	tig0007212:-12452	None:intergenic
GGAGAAAACTTTCAACAGAC+AGG	0.468311	tig0007212:-12458	Msa1345520:CDS
CCACACCATGATTAATCACT+TGG	0.471767	tig0007212:+12708	Msa1345520:intergenic
GGTGGTGTCTCTGGCTACTC+AGG	0.473480	tig0007212:-12596	Msa1345520:CDS
TGGTACATGCAAAATGCAAA+TGG	0.478696	tig0007212:-13016	None:intergenic
ATGCATCATTGATGAGCTCT+TGG	0.485155	tig0007212:+12684	Msa1345520:intergenic
AAAGAAAATGTCTCTTTCCA+TGG	0.522658	tig0007212:+12542	Msa1345520:intergenic
GACTTAAGTGTCATGAAAAG+TGG	0.534486	tig0007212:-12797	Msa1345520:CDS
CAAGTGCTGCTGAGCTTGTG+AGG	0.575589	tig0007212:-12757	Msa1345520:CDS
CTAAGTGCTAAGAGAGTGCA+TGG	0.578017	tig0007212:-12617	Msa1345520:CDS
GATATTCTTCCAAGTTACCA+TGG	0.583162	tig0007212:-12559	Msa1345520:CDS
ATGACACTTAAGTCTATGAG+AGG	0.591503	tig0007212:+12806	Msa1345520:intergenic
AGGAAAGCATGTTTGAAACA+TGG	0.621611	tig0007212:-12737	Msa1345520:CDS
GAGAAAACTTTCAACAGACA+GGG	0.632132	tig0007212:-12457	Msa1345520:CDS
TGATAGGTAGCTTGAAAATG+GGG	0.637100	tig0007212:+12648	Msa1345520:intergenic
TTTAGTTAACACAGCCCAAG+AGG	0.637386	tig0007212:-12840	Msa1345520:CDS
GAAACCCAAAACAGAAAACA+TGG	0.640924	tig0007212:-12936	Msa1345520:CDS
CCAAGTGATTAATCATGGTG+TGG	0.641363	tig0007212:-12708	Msa1345520:CDS
AGTGCTAAGAGAGTGCATGG+TGG	0.823438	tig0007212:-12614	Msa1345520:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTGAAGTACTTAGTTCATT+AGG	+	tig0007212:12478-12497	Msa1333950:intergenic	30.0%
!!!	GTTTCTCTGTTTTTAATGCA+GGG	+	tig0007212:12513-12532	Msa1333950:intergenic	30.0%
!	GAAACCATGTTTTCTGTTTT+GGG	+	tig0007212:12535-12554	Msa1345520:intergenic	30.0%
!	AGAAACCATGTTTTCTGTTT+TGG	+	tig0007212:12536-12555	Msa1345520:intergenic	30.0%
	GTGTTAACTAAATCCATAGA+TGG	+	tig0007212:12616-12635	Msa1345520:intergenic	30.0%
	TTCTTCCAAGTGATTAATCA+TGG	-	tig0007212:12751-12770	Msa1345520:CDS	30.0%
	AAAGAAAATGTCTCTTTCCA+TGG	+	tig0007212:12925-12944	Msa1345520:intergenic	30.0%
!	TCTGAGAATTGGAATCATTT+TGG	+	tig0007212:12960-12979	Msa1345520:intergenic	30.0%
	GTAGTTAACAATCTGAGAAT+TGG	+	tig0007212:12971-12990	Msa1345520:intergenic	30.0%
!	AACTACTTCAAGTCAGTTTT+AGG	-	tig0007212:12985-13004	Msa1345520:CDS	30.0%
!!!	GGTTTCTCTGTTTTTAATGC+AGG	+	tig0007212:12514-12533	Msa1333950:intergenic	35.0%
	GAAACCCAAAACAGAAAACA+TGG	-	tig0007212:12528-12547	Msa1345520:CDS	35.0%
	ACATGCCAAAAGAGTTCATT+TGG	-	tig0007212:12590-12609	Msa1345520:CDS	35.0%
	ATGACACTTAAGTCTATGAG+AGG	+	tig0007212:12661-12680	Msa1345520:intergenic	35.0%
	GACTTAAGTGTCATGAAAAG+TGG	-	tig0007212:12667-12686	Msa1345520:CDS	35.0%
!	AGGAAAGCATGTTTGAAACA+TGG	-	tig0007212:12727-12746	Msa1345520:CDS	35.0%
	TGATAGGTAGCTTGAAAATG+GGG	+	tig0007212:12819-12838	Msa1345520:intergenic	35.0%
	CTGATAGGTAGCTTGAAAAT+GGG	+	tig0007212:12820-12839	Msa1345520:intergenic	35.0%
	GATATTCTTCCAAGTTACCA+TGG	-	tig0007212:12905-12924	Msa1345520:CDS	35.0%
	GAGAAAACTTTCAACAGACA+GGG	-	tig0007212:13007-13026	Msa1345520:CDS	35.0%
!	GATGGCCAAATGAACTCTTT+TGG	+	tig0007212:12598-12617	Msa1345520:intergenic	40.0%
	AGAGTTCATTTGGCCATCTA+TGG	-	tig0007212:12600-12619	Msa1345520:CDS	40.0%
	TTTAGTTAACACAGCCCAAG+AGG	-	tig0007212:12624-12643	Msa1345520:CDS	40.0%
!!!	AGGTTCTTTTAGCTCCTCTT+GGG	+	tig0007212:12641-12660	Msa1345520:intergenic	40.0%
	CCACACCATGATTAATCACT+TGG	+	tig0007212:12759-12778	Msa1345520:intergenic	40.0%
	CCAAGTGATTAATCATGGTG+TGG	-	tig0007212:12756-12775	Msa1345520:CDS	40.0%
	ATGCATCATTGATGAGCTCT+TGG	+	tig0007212:12783-12802	Msa1345520:intergenic	40.0%
	GCTGATAGGTAGCTTGAAAA+TGG	+	tig0007212:12821-12840	Msa1345520:intergenic	40.0%
	TGTCTCTTTCCATGGTAACT+TGG	+	tig0007212:12917-12936	Msa1345520:intergenic	40.0%
	GGAGAAAACTTTCAACAGAC+AGG	-	tig0007212:13006-13025	Msa1345520:CDS	40.0%
!!!	GAGGTTCTTTTAGCTCCTCT+TGG	+	tig0007212:12642-12661	Msa1345520:intergenic	45.0%
	GCACTTAGCTTCTTGCTGAT+AGG	+	tig0007212:12835-12854	Msa1345520:intergenic	45.0%
!	CTAAGTGCTAAGAGAGTGCA+TGG	-	tig0007212:12847-12866	Msa1345520:CDS	45.0%
!	AGTGCTAAGAGAGTGCATGG+TGG	-	tig0007212:12850-12869	Msa1345520:CDS	50.0%
!	CAAGTGCTGCTGAGCTTGTG+AGG	-	tig0007212:12707-12726	Msa1345520:CDS	55.0%
!	AGAGTGCATGGTGGTGTCTC+TGG	-	tig0007212:12859-12878	Msa1345520:CDS	55.0%
	GGTGGTGTCTCTGGCTACTC+AGG	-	tig0007212:12868-12887	Msa1345520:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0007212	gene	12454	13032	12454	ID=Msa1345520;Name=Msa1345520
tig0007212	mRNA	12454	13032	12454	ID=Msa1345520-mRNA-1;Parent=Msa1345520;Name=Msa1345520-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|192
tig0007212	exon	12454	13032	12454	ID=Msa1345520-mRNA-1:exon:5027;Parent=Msa1345520-mRNA-1
tig0007212	CDS	12454	13032	12454	ID=Msa1345520-mRNA-1:cds;Parent=Msa1345520-mRNA-1

Gene Sequence

>Msa1345520

ATGCAAAATGCAAATGGCTCTCCTAATGAACTAAGTACTTCAACTCTTGTACTTCACCCTGCATTAAAAACAGAGAAACCCAAAACAGAAAACATGGTTTCTATTTTTGACTCAAATTTGCTTCAAAATCAAGTGAACATGCCAAAAGAGTTCATTTGGCCATCTATGGATTTAGTTAACACAGCCCAAGAGGAGCTAAAAGAACCTCTCATAGACTTAAGTGTCATGAAAAGTGGTGATGAAGAAGCTATTGCAAGTGCTGCTGAGCTTGTGAGGAAAGCATGTTTGAAACATGGTTTCTTCCAAGTGATTAATCATGGTGTGGACCAAGAGCTCATCAATGATGCATATTGTGAAGTTGACCCCATTTTCAAGCTACCTATCAGCAAGAAGCTAAGTGCTAAGAGAGTGCATGGTGGTGTCTCTGGCTACTCAGGTGCTCATGCAGATAGATATTCTTCCAAGTTACCATGGAAAGAGACATTTTCTTTTGTATACAATCACCAAAATGATTCCAATTCTCAGATTGTTAACTACTTCAAGTCAGTTTTAGGAGAAAACTTTCAACAGACAGGGTAA

Protein sequence

>Msa1345520

MQNANGSPNELSTSTLVLHPALKTEKPKTENMVSIFDSNLLQNQVNMPKEFIWPSMDLVNTAQEELKEPLIDLSVMKSGDEEAIASAAELVRKACLKHGFFQVINHGVDQELINDAYCEVDPIFKLPISKKLSAKRVHGGVSGYSGAHADRYSSKLPWKETFSFVYNHQNDSNSQIVNYFKSVLGENFQQTG*