AlfalfaGEDB | Msa0427500

M. sativa cultivar zhongmu-4 / Msa0427500

KEGG: K05282
GO: GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0045544 GO:0046394 GO:0048367 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0080167 GO:0090567 GO:1901576 GO:1901700 GO:1901701
PFAM: PF14226.9

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427500	AFK38891.1	96.685	181	6	0	12	192	10	190	1.65e-125	365

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427500	sp\|O04706\|GAO1B_WHEAT	42.138	159	89	2	35	192	5	161	7.82e-38	136

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427500	I3SF49	96.685	181	6	0	12	192	10	190	7.88e-126	365

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0378210	Msa0427500	0.890289	1.126908e-73	-8.615850e-47
Msa0378220	Msa0427500	0.993473	5.581337e-200	-8.615850e-47
Msa0400890	Msa0427500	0.800336	1.512124e-48	-8.615850e-47
Msa0427430	Msa0427500	0.881126	3.109660e-70	-8.615850e-47
Msa0427440	Msa0427500	0.989065	1.513043e-176	-8.615850e-47
Msa0427490	Msa0427500	0.888498	5.596988e-73	-8.615850e-47
Msa0427500	Msa0518260	0.945347	3.618933e-104	-8.615850e-47
Msa0427500	Msa0518270	0.894827	1.709144e-75	-8.615850e-47
Msa0427500	Msa0653690	0.817042	4.047463e-52	-8.615850e-47
Msa0427500	Msa1345510	0.863458	2.453876e-64	-8.615850e-47
Msa0427500	Msa1345520	0.989201	4.098600e-177	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427500	MtrunA17_Chr3g0130391	96.685	181	6	0	12	192	10	190	3.98e-128	365
Msa0427500	MtrunA17_Chr1g0191201	53.571	168	75	2	27	192	11	177	4.12e-56	181
Msa0427500	MtrunA17_Chr8g0384481	48.901	182	87	3	11	192	4	179	2.21e-52	172
Msa0427500	MtrunA17_Chr3g0125421	54.605	152	67	2	41	192	10	159	3.47e-51	168
Msa0427500	MtrunA17_Chr8g0384461	50.602	166	78	2	28	192	6	168	6.16e-50	165
Msa0427500	MtrunA17_Chr1g0204181	42.405	158	91	0	35	192	26	183	2.40e-41	143
Msa0427500	MtrunA17_Chr6g0474391	39.227	181	110	0	12	192	8	188	1.52e-40	141
Msa0427500	MtrunA17_Chr8g0351101	41.243	177	101	3	17	192	3	177	1.45e-37	133
Msa0427500	MtrunA17_Chr7g0239101	34.343	99	60	2	69	166	56	150	1.08e-12	65.5
Msa0427500	MtrunA17_Chr8g0375661	31.148	122	82	1	46	165	28	149	3.49e-12	62.0
Msa0427500	MtrunA17_Chr8g0375641	29.508	122	84	1	46	165	28	149	5.68e-12	61.6
Msa0427500	MtrunA17_Chr2g0332191	31.818	132	75	3	37	165	21	140	7.10e-12	63.2
Msa0427500	MtrunA17_Chr7g0233671	31.579	114	76	1	64	175	37	150	1.31e-11	60.1
Msa0427500	MtrunA17_Chr8g0375671	29.508	122	84	1	46	165	28	149	1.49e-11	62.0
Msa0427500	MtrunA17_Chr5g0423191	29.268	123	81	3	42	162	19	137	2.40e-11	61.6
Msa0427500	MtrunA17_Chr7g0239121	33.333	102	63	2	69	169	52	149	3.33e-11	61.2
Msa0427500	MtrunA17_Chr5g0398291	30.952	126	78	3	46	162	18	143	3.60e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0427500	AT5G07200.1	40.217	184	107	3	11	192	1	183	8.98e-38	134
Msa0427500	AT5G51810.2	37.572	173	101	2	20	192	17	182	6.41e-36	127
Msa0427500	AT5G51810.1	37.572	173	101	2	20	192	17	182	3.77e-35	127
Msa0427500	AT4G25420.2	39.873	158	94	1	35	192	28	184	1.25e-34	124
Msa0427500	AT4G25420.1	39.873	158	94	1	35	192	28	184	5.19e-34	124
Msa0427500	AT1G60980.1	37.725	167	100	3	30	192	18	184	7.56e-31	115
Msa0427500	AT1G44090.1	37.209	172	104	3	22	192	17	185	5.68e-30	113
Msa0427500	AT1G80340.1	34.375	128	73	3	46	171	26	144	1.54e-14	70.9
Msa0427500	AT4G16770.1	36.607	112	59	4	57	164	11	114	1.67e-11	61.6

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAGAATTGGAATCATTT+TGG	0.198738	3_2:+78170639	None:intergenic
GTTGAAGTACTTAGTTCATT+AGG	0.274284	3_2:+78171121	None:intergenic
GCTGATAGGTAGCTTGAAAA+TGG	0.275391	3_2:+78170778	None:intergenic
AGAGTTCATTTGGCCATCTA+TGG	0.293217	3_2:-78170996	Msa0427500:CDS
GATGGCCAAATGAACTCTTT+TGG	0.339326	3_2:+78171001	None:intergenic
GTAGTTAACAATCTGAGAAT+TGG	0.352416	3_2:+78170628	None:intergenic
ACATGCCAAAAGAGTTCATT+TGG	0.399130	3_2:-78171006	Msa0427500:CDS
TGTCTCTTTCCATGGTAACT+TGG	0.412911	3_2:+78170682	None:intergenic
TTCTTCCAAGTGATTAATCA+TGG	0.415834	3_2:-78170845	Msa0427500:CDS
AGAGTGCATGGTGGTGTCTC+TGG	0.415871	3_2:-78170737	Msa0427500:CDS
GTGTTAACTAAATCCATAGA+TGG	0.436495	3_2:+78170983	None:intergenic
GCACTTAGCTTCTTGCTGAT+AGG	0.441284	3_2:+78170764	None:intergenic
CTGATAGGTAGCTTGAAAAT+GGG	0.444807	3_2:+78170779	None:intergenic
AACTTTCAACAGACAGGGTA+AGG	0.463250	3_2:-78170584	None:intergenic
GGAGAAAACTTTCAACAGAC+AGG	0.468311	3_2:-78170590	Msa0427500:CDS
CCACACCATGATTAATCACT+TGG	0.471767	3_2:+78170840	None:intergenic
GGTGGTGTCTCTGGCTACTC+AGG	0.473480	3_2:-78170728	Msa0427500:CDS
TGGTACATGCAAAATGCAAA+TGG	0.478696	3_2:-78171148	None:intergenic
ATGCATCATTGATGAGCTCT+TGG	0.485155	3_2:+78170816	None:intergenic
AAAGAAAATGTCTCTTTCCA+TGG	0.522658	3_2:+78170674	None:intergenic
GACTTAAGTGTCATGAAAAG+TGG	0.534486	3_2:-78170929	Msa0427500:CDS
CAAGTGCTGCTGAGCTTGTG+AGG	0.575589	3_2:-78170889	Msa0427500:CDS
CTAAGTGCTAAGAGAGTGCA+TGG	0.578017	3_2:-78170749	Msa0427500:CDS
GATATTCTTCCAAGTTACCA+TGG	0.583162	3_2:-78170691	Msa0427500:CDS
ATGACACTTAAGTCTATGAG+AGG	0.591503	3_2:+78170938	None:intergenic
AGGAAAGCATGTTTGAAACA+TGG	0.621611	3_2:-78170869	Msa0427500:CDS
GAGAAAACTTTCAACAGACA+GGG	0.632132	3_2:-78170589	Msa0427500:CDS
TGATAGGTAGCTTGAAAATG+GGG	0.637100	3_2:+78170780	None:intergenic
TTTAGTTAACACAGCCCAAG+AGG	0.637386	3_2:-78170972	Msa0427500:CDS
GAAACCCAAAACAGAAAACA+TGG	0.640924	3_2:-78171068	Msa0427500:CDS
CCAAGTGATTAATCATGGTG+TGG	0.641363	3_2:-78170840	Msa0427500:CDS
AGTGCTAAGAGAGTGCATGG+TGG	0.823438	3_2:-78170746	Msa0427500:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTTGAAGTACTTAGTTCATT+AGG	+	chr3_2:78170610-78170629	None:intergenic	30.0%
!!!	GTTTCTCTGTTTTTAATGCA+GGG	+	chr3_2:78170645-78170664	None:intergenic	30.0%
!	GAAACCATGTTTTCTGTTTT+GGG	+	chr3_2:78170667-78170686	None:intergenic	30.0%
!	AGAAACCATGTTTTCTGTTT+TGG	+	chr3_2:78170668-78170687	None:intergenic	30.0%
	GTGTTAACTAAATCCATAGA+TGG	+	chr3_2:78170748-78170767	None:intergenic	30.0%
	TTCTTCCAAGTGATTAATCA+TGG	-	chr3_2:78170883-78170902	Msa0427500:CDS	30.0%
	AAAGAAAATGTCTCTTTCCA+TGG	+	chr3_2:78171057-78171076	None:intergenic	30.0%
!	TCTGAGAATTGGAATCATTT+TGG	+	chr3_2:78171092-78171111	None:intergenic	30.0%
	GTAGTTAACAATCTGAGAAT+TGG	+	chr3_2:78171103-78171122	None:intergenic	30.0%
!	AACTACTTCAAGTCAGTTTT+AGG	-	chr3_2:78171117-78171136	Msa0427500:CDS	30.0%
!!!	GGTTTCTCTGTTTTTAATGC+AGG	+	chr3_2:78170646-78170665	None:intergenic	35.0%
	GAAACCCAAAACAGAAAACA+TGG	-	chr3_2:78170660-78170679	Msa0427500:CDS	35.0%
	ACATGCCAAAAGAGTTCATT+TGG	-	chr3_2:78170722-78170741	Msa0427500:CDS	35.0%
	ATGACACTTAAGTCTATGAG+AGG	+	chr3_2:78170793-78170812	None:intergenic	35.0%
	GACTTAAGTGTCATGAAAAG+TGG	-	chr3_2:78170799-78170818	Msa0427500:CDS	35.0%
!	AGGAAAGCATGTTTGAAACA+TGG	-	chr3_2:78170859-78170878	Msa0427500:CDS	35.0%
	TGATAGGTAGCTTGAAAATG+GGG	+	chr3_2:78170951-78170970	None:intergenic	35.0%
	CTGATAGGTAGCTTGAAAAT+GGG	+	chr3_2:78170952-78170971	None:intergenic	35.0%
	GATATTCTTCCAAGTTACCA+TGG	-	chr3_2:78171037-78171056	Msa0427500:CDS	35.0%
	GAGAAAACTTTCAACAGACA+GGG	-	chr3_2:78171139-78171158	Msa0427500:CDS	35.0%
!	GATGGCCAAATGAACTCTTT+TGG	+	chr3_2:78170730-78170749	None:intergenic	40.0%
	AGAGTTCATTTGGCCATCTA+TGG	-	chr3_2:78170732-78170751	Msa0427500:CDS	40.0%
	TTTAGTTAACACAGCCCAAG+AGG	-	chr3_2:78170756-78170775	Msa0427500:CDS	40.0%
!!!	AGGTTCTTTTAGCTCCTCTT+GGG	+	chr3_2:78170773-78170792	None:intergenic	40.0%
	CCACACCATGATTAATCACT+TGG	+	chr3_2:78170891-78170910	None:intergenic	40.0%
	CCAAGTGATTAATCATGGTG+TGG	-	chr3_2:78170888-78170907	Msa0427500:CDS	40.0%
	ATGCATCATTGATGAGCTCT+TGG	+	chr3_2:78170915-78170934	None:intergenic	40.0%
	GCTGATAGGTAGCTTGAAAA+TGG	+	chr3_2:78170953-78170972	None:intergenic	40.0%
	TGTCTCTTTCCATGGTAACT+TGG	+	chr3_2:78171049-78171068	None:intergenic	40.0%
	GGAGAAAACTTTCAACAGAC+AGG	-	chr3_2:78171138-78171157	Msa0427500:CDS	40.0%
!!!	GAGGTTCTTTTAGCTCCTCT+TGG	+	chr3_2:78170774-78170793	None:intergenic	45.0%
	GCACTTAGCTTCTTGCTGAT+AGG	+	chr3_2:78170967-78170986	None:intergenic	45.0%
!	CTAAGTGCTAAGAGAGTGCA+TGG	-	chr3_2:78170979-78170998	Msa0427500:CDS	45.0%
!	AGTGCTAAGAGAGTGCATGG+TGG	-	chr3_2:78170982-78171001	Msa0427500:CDS	50.0%
!	CAAGTGCTGCTGAGCTTGTG+AGG	-	chr3_2:78170839-78170858	Msa0427500:CDS	55.0%
!	AGAGTGCATGGTGGTGTCTC+TGG	-	chr3_2:78170991-78171010	Msa0427500:CDS	55.0%
	GGTGGTGTCTCTGGCTACTC+AGG	-	chr3_2:78171000-78171019	Msa0427500:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	78170586	78171164	78170586	ID=Msa0427500;Name=Msa0427500
chr3_2	mRNA	78170586	78171164	78170586	ID=Msa0427500-mRNA-1;Parent=Msa0427500;Name=Msa0427500-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|192
chr3_2	exon	78170586	78171164	78170586	ID=Msa0427500-mRNA-1:exon:20526;Parent=Msa0427500-mRNA-1
chr3_2	CDS	78170586	78171164	78170586	ID=Msa0427500-mRNA-1:cds;Parent=Msa0427500-mRNA-1

Gene Sequence

>Msa0427500

ATGCAAAATGCAAATGGCTCTCCTAATGAACTAAGTACTTCAACTCTTGTACTTCACCCTGCATTAAAAACAGAGAAACCCAAAACAGAAAACATGGTTTCTATTTTTGACTCAAATTTGCTTCAAAATCAAGTGAACATGCCAAAAGAGTTCATTTGGCCATCTATGGATTTAGTTAACACAGCCCAAGAGGAGCTAAAAGAACCTCTCATAGACTTAAGTGTCATGAAAAGTGGTGATGAAGAAGCTATTGCAAGTGCTGCTGAGCTTGTGAGGAAAGCATGTTTGAAACATGGTTTCTTCCAAGTGATTAATCATGGTGTGGACCAAGAGCTCATCAATGATGCATATTGTGAAGTTGACCCCATTTTCAAGCTACCTATCAGCAAGAAGCTAAGTGCTAAGAGAGTGCATGGTGGTGTCTCTGGCTACTCAGGTGCTCATGCAGATAGATATTCTTCCAAGTTACCATGGAAAGAGACATTTTCTTTTGTATACAATCACCAAAATGATTCCAATTCTCAGATTGTTAACTACTTCAAGTCAGTTTTAGGAGAAAACTTTCAACAGACAGGGTAA

Protein sequence

>Msa0427500

MQNANGSPNELSTSTLVLHPALKTEKPKTENMVSIFDSNLLQNQVNMPKEFIWPSMDLVNTAQEELKEPLIDLSVMKSGDEEAIASAAELVRKACLKHGFFQVINHGVDQELINDAYCEVDPIFKLPISKKLSAKRVHGGVSGYSGAHADRYSSKLPWKETFSFVYNHQNDSNSQIVNYFKSVLGENFQQTG*