AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0526970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0526970 MtrunA17_Chr7g0270871 90.256 195 12 3 4 195 183 373 1.61e-113 328
Msa0526970 MtrunA17_Chr1g0193321 59.615 104 39 1 5 108 444 544 8.03e-32 120
Msa0526970 MtrunA17_Chr1g0182791 65.333 75 26 0 14 88 308 382 6.41e-28 109
Msa0526970 MtrunA17_Chr3g0095391 46.018 113 54 3 3 114 290 396 2.01e-24 99.4
Msa0526970 MtrunA17_Chr7g0271251 47.826 138 52 5 6 135 413 538 2.86e-24 99.4
Msa0526970 MtrunA17_Chr7g0262691 56.180 89 32 2 6 88 236 323 1.97e-23 96.7
Msa0526970 MtrunA17_Chr1g0153241 56.627 83 34 2 3 85 67 147 4.16e-22 90.5
Msa0526970 MtrunA17_Chr5g0402031 56.716 67 29 0 32 98 114 180 6.33e-20 85.9
Msa0526970 MtrunA17_Chr7g0231461 51.250 80 35 2 9 85 281 359 8.12e-17 77.8
Msa0526970 MtrunA17_Chr4g0074161 55.357 56 25 0 34 89 217 272 9.55e-17 77.4
Msa0526970 MtrunA17_Chr1g0190731 51.786 56 27 0 34 89 204 259 4.92e-16 75.1
Msa0526970 MtrunA17_Chr3g0134741 36.735 98 53 2 20 115 224 314 1.09e-13 68.2
Msa0526970 MtrunA17_Chr1g0152131 46.970 66 34 1 34 98 271 336 2.80e-13 67.4
Msa0526970 MtrunA17_Chr1g0207301 34.615 78 44 1 39 116 304 374 1.01e-12 65.9
Msa0526970 MtrunA17_Chr4g0054581 45.588 68 37 0 18 85 183 250 1.44e-12 65.1
Msa0526970 MtrunA17_Chr3g0144641 35.780 109 61 3 34 134 202 309 3.51e-12 64.3
Msa0526970 MtrunA17_Chr4g0002781 44.872 78 43 0 6 83 147 224 4.76e-12 63.5
Msa0526970 MtrunA17_Chr5g0421111 43.939 66 36 1 34 98 376 441 6.24e-12 63.5
Msa0526970 MtrunA17_Chr8g0346151 44.643 56 31 0 34 89 280 335 6.71e-12 63.5
Msa0526970 MtrunA17_Chr4g0035101 40.000 80 46 2 16 94 137 215 7.12e-12 63.2
Msa0526970 MtrunA17_Chr1g0207311 29.524 105 62 2 19 118 187 284 7.42e-12 62.8
Msa0526970 MtrunA17_Chr8g0365941 44.118 68 37 1 34 100 316 383 9.74e-12 62.8
Msa0526970 MtrunA17_Chr8g0363921 45.455 55 29 1 34 87 162 216 1.11e-11 62.0
Msa0526970 MtrunA17_Chr3g0134001 49.180 61 27 1 29 85 173 233 1.16e-11 62.0
Msa0526970 MtrunA17_Chr5g0400661 44.444 63 35 0 23 85 139 201 1.72e-11 62.0
Msa0526970 MtrunA17_Chr8g0384141 47.541 61 28 1 29 85 116 176 1.76e-11 61.6
Msa0526970 MtrunA17_Chr5g0393721 32.530 83 49 1 34 116 235 310 1.86e-11 62.0
Msa0526970 MtrunA17_Chr7g0234761 46.552 58 30 1 34 90 328 385 2.24e-11 62.0
Msa0526970 MtrunA17_Chr8g0339091 47.273 55 28 1 34 87 302 356 2.43e-11 62.0
Msa0526970 MtrunA17_Chr7g0275821 48.214 56 29 0 34 89 253 308 2.64e-11 61.6
Msa0526970 MtrunA17_Chr5g0415291 45.455 55 29 1 34 87 123 177 2.88e-11 60.5
Msa0526970 MtrunA17_Chr8g0366751 42.029 69 40 0 15 83 444 512 4.89e-11 60.8
Msa0526970 MtrunA17_Chr5g0400621 41.026 78 46 0 9 86 133 210 6.10e-11 60.5
Msa0526970 MtrunA17_Chr8g0346161 48.000 50 26 0 34 83 292 341 9.57e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0526970 AT2G20180.7 41.317 167 83 3 6 169 190 344 5.40e-30 114
Msa0526970 AT2G20180.1 41.317 167 83 3 6 169 190 344 5.40e-30 114
Msa0526970 AT2G20180.5 41.317 167 83 3 6 169 190 344 5.40e-30 114
Msa0526970 AT1G09530.3 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT1G09530.2 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT1G09530.5 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT1G09530.1 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT1G09530.4 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT1G09530.6 60.000 90 35 1 5 94 320 408 1.29e-29 114
Msa0526970 AT2G20180.4 40.964 166 85 2 6 169 261 415 2.12e-29 113
Msa0526970 AT2G20180.6 40.964 166 85 2 6 169 261 415 2.12e-29 113
Msa0526970 AT2G20180.2 40.964 166 85 2 6 169 261 415 2.12e-29 113
Msa0526970 AT2G20180.3 40.964 166 85 2 6 169 261 415 2.12e-29 113
Msa0526970 AT2G46970.2 41.071 168 86 5 4 171 179 333 7.45e-28 108
Msa0526970 AT2G46970.3 41.071 168 86 5 4 171 188 342 8.67e-28 108
Msa0526970 AT2G46970.1 41.071 168 86 5 4 171 210 364 1.12e-27 108
Msa0526970 AT2G20180.8 54.430 79 36 0 6 84 261 339 6.98e-24 97.1
Msa0526970 AT5G67110.1 45.045 111 59 2 14 123 79 188 1.24e-23 93.6
Msa0526970 AT4G28800.1 41.353 133 73 2 7 139 234 361 6.09e-23 95.5
Msa0526970 AT3G59060.1 37.037 189 104 7 8 186 236 419 1.20e-21 91.7
Msa0526970 AT3G59060.3 38.922 167 92 5 8 166 236 400 2.25e-21 90.9
Msa0526970 AT3G59060.2 38.922 167 92 5 8 166 236 400 2.25e-21 90.9
Msa0526970 AT3G59060.4 38.922 167 92 5 8 166 236 400 2.25e-21 90.9
Msa0526970 AT4G28790.1 39.855 138 67 4 6 139 255 380 4.13e-21 90.1
Msa0526970 AT4G36930.1 57.746 71 25 1 29 94 197 267 1.68e-20 88.2
Msa0526970 AT4G28800.2 41.176 119 60 3 21 139 259 367 2.68e-19 85.1
Msa0526970 AT2G43010.1 68.333 60 19 0 26 85 254 313 8.38e-19 83.6
Msa0526970 AT2G43010.2 68.333 60 19 0 26 85 254 313 8.56e-19 83.6
Msa0526970 AT2G43010.3 68.333 60 19 0 26 85 261 320 1.01e-18 83.6
Msa0526970 AT2G43010.4 68.333 60 19 0 26 85 254 313 1.05e-18 83.2
Msa0526970 AT2G43010.5 68.333 60 19 0 26 85 296 355 1.57e-18 83.2
Msa0526970 AT4G28815.2 42.857 91 35 2 21 111 66 139 3.34e-18 79.7
Msa0526970 AT3G62090.2 35.593 177 95 7 17 187 176 339 3.63e-18 81.3
Msa0526970 AT3G62090.1 35.593 177 95 7 17 187 159 322 3.65e-18 81.3
Msa0526970 AT4G00050.2 56.000 75 30 2 14 86 95 168 4.41e-18 80.5
Msa0526970 AT2G24260.2 57.143 56 24 0 34 89 46 101 6.54e-18 79.3
Msa0526970 AT4G00050.3 56.000 75 30 2 14 86 197 270 6.77e-18 80.9
Msa0526970 AT4G00050.1 56.000 75 30 2 14 86 197 270 7.08e-18 80.9
Msa0526970 AT4G28815.1 42.857 91 35 2 21 111 142 215 9.61e-18 79.7
Msa0526970 AT2G24260.1 57.143 56 24 0 34 89 149 204 2.92e-17 79.0
Msa0526970 AT4G28790.2 50.000 78 38 1 6 83 255 331 3.66e-17 78.6
Msa0526970 AT4G30980.1 55.357 56 25 0 34 89 141 196 9.17e-17 77.0
Msa0526970 AT4G30980.2 55.357 56 25 0 34 89 189 244 1.71e-16 76.6
Msa0526970 AT5G58010.1 43.373 83 41 2 8 89 88 165 3.27e-16 75.5
Msa0526970 AT4G28811.1 48.276 87 40 2 4 86 329 414 8.71e-14 69.3
Msa0526970 AT2G14760.1 32.710 107 63 2 14 118 226 325 4.02e-13 67.0
Msa0526970 AT5G61270.2 45.882 85 42 1 3 87 56 136 4.86e-13 66.2
Msa0526970 AT5G61270.3 45.882 85 42 1 3 87 56 136 4.86e-13 66.2
Msa0526970 AT5G50915.3 39.326 89 52 2 9 95 121 209 6.70e-13 65.9
Msa0526970 AT5G50915.4 39.326 89 52 2 9 95 121 209 6.70e-13 65.9
Msa0526970 AT5G61270.1 45.882 85 42 1 3 87 144 224 7.72e-13 66.2
Msa0526970 AT1G10120.2 48.387 62 31 1 34 94 217 278 8.26e-13 66.2
Msa0526970 AT1G10120.1 48.387 62 31 1 34 94 217 278 8.26e-13 66.2
Msa0526970 AT5G50915.1 39.326 89 52 2 9 95 121 209 1.04e-12 65.5
Msa0526970 AT5G50915.2 39.326 89 52 2 9 95 121 209 1.04e-12 65.5
Msa0526970 AT1G02340.2 34.965 143 64 7 1 133 112 235 1.11e-12 65.5
Msa0526970 AT2G14760.3 33.333 87 51 1 32 118 259 338 1.34e-12 65.5
Msa0526970 AT1G02340.1 34.965 143 64 7 1 133 119 242 1.41e-12 65.1
Msa0526970 AT5G43175.1 34.940 83 47 1 34 116 143 218 3.53e-12 63.2
Msa0526970 AT1G68920.1 43.939 66 36 1 34 98 314 379 4.98e-12 64.3
Msa0526970 AT1G68920.4 43.939 66 36 1 34 98 314 379 4.98e-12 64.3
Msa0526970 AT1G68920.2 43.939 66 36 1 34 98 314 379 4.98e-12 64.3
Msa0526970 AT1G68920.3 43.939 66 36 1 34 98 313 378 5.07e-12 64.3
Msa0526970 AT4G36540.1 35.227 88 55 2 8 94 127 213 9.60e-12 62.8
Msa0526970 AT1G26260.5 42.424 66 37 1 34 98 212 277 9.74e-12 63.2
Msa0526970 AT1G26260.4 42.424 66 37 1 34 98 234 299 9.87e-12 63.2
Msa0526970 AT1G26260.1 42.424 66 37 1 34 98 234 299 9.87e-12 63.2
Msa0526970 AT1G26260.6 42.424 66 37 1 34 98 234 299 9.87e-12 63.2
Msa0526970 AT1G26260.2 42.424 66 37 1 34 98 234 299 9.87e-12 63.2
Msa0526970 AT4G34530.1 41.176 68 39 1 34 100 183 250 1.06e-11 63.2
Msa0526970 AT4G36540.2 35.227 88 55 2 8 94 127 213 1.13e-11 62.8
Msa0526970 AT5G62610.2 49.091 55 27 1 34 87 164 218 1.22e-11 62.4
Msa0526970 AT5G62610.1 49.091 55 27 1 34 87 164 218 1.22e-11 62.4
Msa0526970 AT1G25330.1 39.286 84 46 3 23 102 105 187 1.24e-11 61.6
Msa0526970 AT1G26260.3 42.424 66 37 1 34 98 183 248 1.30e-11 62.8
Msa0526970 AT2G46510.1 50.000 52 26 0 34 85 396 447 1.31e-11 63.2
Msa0526970 AT2G18300.2 43.548 62 34 1 34 94 196 257 1.80e-11 62.4
Msa0526970 AT2G18300.1 43.548 62 34 1 34 94 196 257 1.80e-11 62.4
Msa0526970 AT3G23690.1 42.424 66 37 1 34 98 202 267 1.82e-11 62.4
Msa0526970 AT1G59640.1 45.455 55 29 1 34 87 147 201 2.02e-11 61.6
Msa0526970 AT2G18300.3 43.548 62 34 1 34 94 196 257 2.32e-11 62.0
Msa0526970 AT1G01260.1 39.189 74 45 0 16 89 416 489 3.36e-11 62.0
Msa0526970 AT1G01260.2 39.189 74 45 0 16 89 416 489 3.36e-11 62.0
Msa0526970 AT1G01260.3 39.189 74 45 0 16 89 416 489 3.36e-11 62.0
Msa0526970 AT5G48560.2 47.273 55 28 1 34 87 312 366 3.48e-11 61.6
Msa0526970 AT3G07340.2 47.273 55 28 1 34 87 269 323 3.62e-11 61.6
Msa0526970 AT5G48560.1 47.273 55 28 1 34 87 312 366 3.66e-11 61.6
Msa0526970 AT3G07340.1 47.273 55 28 1 34 87 269 323 4.24e-11 61.6
Msa0526970 AT1G63650.3 37.037 81 40 1 9 89 392 461 4.28e-11 61.6
Msa0526970 AT1G63650.1 37.037 81 40 1 9 89 392 461 4.28e-11 61.6
Msa0526970 AT1G63650.2 37.037 81 40 1 9 89 392 461 4.28e-11 61.6
Msa0526970 AT4G00120.1 49.180 61 27 1 29 85 114 174 4.65e-11 59.7
Msa0526970 AT4G16430.1 36.585 82 52 0 3 84 290 371 5.23e-11 61.2
Msa0526970 AT1G18400.2 47.273 55 28 1 34 87 156 210 5.41e-11 60.5
Msa0526970 AT1G18400.1 47.273 55 28 1 34 87 156 210 5.74e-11 60.5
Msa0526970 AT1G59640.2 45.455 55 29 1 34 87 147 201 6.69e-11 60.8
Msa0526970 AT4G33880.1 42.254 71 39 1 18 86 259 329 7.61e-11 60.5
Msa0526970 AT1G68240.1 38.667 75 45 1 15 88 53 127 8.95e-11 58.9

Find 117 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACAACATTTGATGGGTTTC+AGG 0.156734 3_4:-81121073 Msa0526970:CDS
TTTCTCTTTCCTCTAATTCT+AGG 0.178319 3_4:+81122073 None:intergenic
GACCCTCAAGCTTCAGTTAC+AGG 0.189644 3_4:-81121302 Msa0526970:intron
TCACATACCTTATTGCAATT+TGG 0.224679 3_4:+81121482 None:intergenic
TTGGATACCATGTTGAAAAT+TGG 0.242044 3_4:+81120239 None:intergenic
CTAGCTATCCTTTCCTTGAT+TGG 0.260539 3_4:-81120626 Msa0526970:three_prime_UTR
GAGCCTATGCATAATATTCT+TGG 0.269072 3_4:+81120822 None:intergenic
AGTTTGTGTACGTTGTTTCT+AGG 0.282978 3_4:+81122578 None:intergenic
TTGACATGTGTGGTGGTAAT+TGG 0.291651 3_4:+81120964 None:intergenic
ACACCAACACCAACCTCTTT+TGG 0.299511 3_4:-81120894 Msa0526970:CDS
TTCTGTGCAATTAGTGTGTT+TGG 0.311610 3_4:-81120701 Msa0526970:three_prime_UTR
CCTTTCATTCCTCCTATCAT+TGG 0.324461 3_4:-81120930 Msa0526970:CDS
CTTACATCATCACTTAAATA+TGG 0.331025 3_4:+81121935 None:intergenic
CATGTTGCTAATGAAGTAAA+TGG 0.334017 3_4:+81122528 None:intergenic
ATGTCCTTCCAGGTTGATTC+TGG 0.340577 3_4:+81120868 None:intergenic
GACTAGCCACATGTCCTTCC+AGG 0.347583 3_4:+81120858 None:intergenic
ACCAATGCACCTAGAATTAG+AGG 0.347789 3_4:-81122082 Msa0526970:five_prime_UTR
CCATCACCCATTTGTGGAAT+AGG 0.357478 3_4:+81121035 None:intergenic
AACATCATAAAATGATTAAC+TGG 0.360190 3_4:+81121146 None:intergenic
TTCATATGCAGAGTTTGATT+TGG 0.364667 3_4:+81122162 None:intergenic
TGTGAGTACTATTCTTACTT+TGG 0.374808 3_4:+81122434 None:intergenic
GTTTGGTTTGAATCCAATCT+TGG 0.379031 3_4:+81122382 None:intergenic
AACGACAGAGTTCAGATGTT+TGG 0.380475 3_4:-81120996 Msa0526970:CDS
TATGAATGCACAACATTTGA+TGG 0.383363 3_4:-81121081 Msa0526970:CDS
GTGAGTACTATTCTTACTTT+GGG 0.386165 3_4:+81122435 None:intergenic
ACAGTTTCCTATTCCACAAA+TGG 0.388703 3_4:-81121042 Msa0526970:CDS
TCCTCTAATTCTAGGTGCAT+TGG 0.390333 3_4:+81122081 None:intergenic
GCTCATACCAAATTGCAATA+AGG 0.391698 3_4:-81121489 Msa0526970:intron
CTAGATTTCTTGAAATATGT+TGG 0.391916 3_4:+81120302 None:intergenic
TTGAATCATTAAAGAAAAGT+AGG 0.394350 3_4:-81120661 Msa0526970:three_prime_UTR
AACATATAAGTGAGCGGAAA+AGG 0.400492 3_4:+81120423 None:intergenic
GACGTTGCAGATTATGTCAA+TGG 0.409902 3_4:-81121186 Msa0526970:intron
AAAAGAGACATTTGATATGT+TGG 0.414165 3_4:-81120758 Msa0526970:three_prime_UTR
AGCCTATGCATAATATTCTT+GGG 0.415276 3_4:+81120823 None:intergenic
AAACAACGTACACAAACTTT+AGG 0.416300 3_4:-81122573 Msa0526970:five_prime_UTR
ACCAAAGTTTAGATCATTTG+AGG 0.426290 3_4:+81121991 None:intergenic
GTGAGTTTGTTGTTGCAGTT+TGG 0.430149 3_4:+81122365 None:intergenic
AAAGAGACATTTGATATGTT+GGG 0.434907 3_4:-81120757 Msa0526970:three_prime_UTR
AATTCATAATTTATCTGAAA+GGG 0.436637 3_4:-81121662 Msa0526970:intron
GCCTCAAATGATCTAAACTT+TGG 0.436672 3_4:-81121992 Msa0526970:five_prime_UTR
ATTGAAACTGTGTCTGTGTC+TGG 0.439883 3_4:+81121835 None:intergenic
ACCAACTTTGATACTATTGT+GGG 0.440078 3_4:-81120530 Msa0526970:three_prime_UTR
TTGATTCTGGTTACCAAAAG+AGG 0.453744 3_4:+81120881 None:intergenic
ACTCTGTTTCCATTATGAGC+TGG 0.454011 3_4:+81121709 None:intergenic
CTCACAGAAACAGTACTTGA+TGG 0.454187 3_4:-81122347 Msa0526970:five_prime_UTR
TGTGTACGTTGTTTCTAGGT+TGG 0.455594 3_4:+81122582 None:intergenic
AAAGAAAAGCCAGCTCATAA+TGG 0.458159 3_4:-81121718 Msa0526970:CDS
CACCAACTTTGATACTATTG+TGG 0.465880 3_4:-81120531 Msa0526970:three_prime_UTR
AGGAGGAATGAAAGGTGCAT+TGG 0.466560 3_4:+81120938 None:intergenic
TGAATCATTAAAGAAAAGTA+GGG 0.467598 3_4:-81120660 Msa0526970:three_prime_UTR
GGAGGAATGAAAGGTGCATT+GGG 0.473309 3_4:+81120939 None:intergenic
TACAAACGTAAAATAGCATA+TGG 0.474481 3_4:+81121898 None:intergenic
GTGGAAGAGTTTCCAATGAT+AGG 0.480699 3_4:+81120918 None:intergenic
TACCTGTAACTGAAGCTTGA+GGG 0.481997 3_4:+81121300 None:intergenic
GTGACACCATCACCCATTTG+TGG 0.484725 3_4:+81121029 None:intergenic
CAATCAAGGAAAGGATAGCT+AGG 0.490800 3_4:+81120627 None:intergenic
CAGTTTCCTATTCCACAAAT+GGG 0.491361 3_4:-81121041 Msa0526970:CDS
ATGCATAATATTCTTGGGCA+TGG 0.495441 3_4:+81120828 None:intergenic
GATACTATTGTGGGATTGCG+AGG 0.504119 3_4:-81120521 Msa0526970:three_prime_UTR
AGTGTAAGTTTGAACATGAA+AGG 0.504506 3_4:-81120185 Msa0526970:three_prime_UTR
TAATTCTAGGTGCATTGGTA+TGG 0.508040 3_4:+81122086 None:intergenic
TGAAGAAACACAAGAAAATA+CGG 0.510993 3_4:-81121743 Msa0526970:CDS
ATCAAGCTTCGTAAGAATGA+TGG 0.511511 3_4:+81121787 None:intergenic
AATTTACCTACCAACAAAAG+AGG 0.512256 3_4:-81120332 Msa0526970:three_prime_UTR
TGGTGTTAGAAAATCATATG+AGG 0.518657 3_4:-81121972 Msa0526970:five_prime_UTR
GAATTGCGAAATACAGTAGC+TGG 0.520468 3_4:+81120721 None:intergenic
TATTTAAGTGATGATGTAAG+TGG 0.521909 3_4:-81121932 Msa0526970:five_prime_UTR
TCAAGTGAGCATACAGAAGA+TGG 0.531560 3_4:+81122016 None:intergenic
ACAGAGTCAAGAGAAGCTAC+AGG 0.536275 3_4:-81121693 Msa0526970:CDS
TCACAGAAACAGTACTTGAT+GGG 0.539019 3_4:-81122346 Msa0526970:five_prime_UTR
ACGTTGTTTCTAGGTTGGAC+TGG 0.539239 3_4:+81122587 None:intergenic
ATGAATGCACAACATTTGAT+GGG 0.539585 3_4:-81121080 Msa0526970:CDS
TCCCACAATAGTATCAAAGT+TGG 0.540946 3_4:+81120529 None:intergenic
AAATTCATAATTTATCTGAA+AGG 0.542829 3_4:-81121663 Msa0526970:CDS
ACGTTGCAGATTATGTCAAT+GGG 0.542904 3_4:-81121185 Msa0526970:intron
AGTACTATTCTTACTTTGGG+AGG 0.546973 3_4:+81122438 None:intergenic
GGAATAGGAAACTGTATTTG+TGG 0.547372 3_4:+81121050 None:intergenic
TGCATAATATTCTTGGGCAT+GGG 0.547979 3_4:+81120829 None:intergenic
CATGTGTGGTGGTAATTGGT+TGG 0.548002 3_4:+81120968 None:intergenic
TTACTATGAAGCACGAACAC+TGG 0.549159 3_4:-81121870 Msa0526970:five_prime_UTR
AGAGGTTGGTGTTGGTGTGG+TGG 0.551444 3_4:+81120899 None:intergenic
TTCATGTTCATATGATGTGC+GGG 0.552429 3_4:+81121122 None:intergenic
ATGACAAGAAGCAATGGCCT+AGG 0.554224 3_4:-81122484 Msa0526970:five_prime_UTR
CCTATTCCACAAATGGGTGA+TGG 0.561913 3_4:-81121035 Msa0526970:CDS
TTGATGTTGTGAGCCAATCA+AGG 0.563413 3_4:+81120613 None:intergenic
TTCTGGTTACCAAAAGAGGT+TGG 0.567323 3_4:+81120885 None:intergenic
TCAGAATGTTCATCAACGGT+TGG 0.575923 3_4:+81122121 None:intergenic
AGCTTCAGAATGTTCATCAA+CGG 0.575933 3_4:+81122117 None:intergenic
TTTGGTAACCAGAATCAACC+TGG 0.577623 3_4:-81120876 Msa0526970:CDS
CTCATTCAAGACGTAAGACA+AGG 0.579237 3_4:+81120270 None:intergenic
AGAATACTCACTCGCACATG+TGG 0.586661 3_4:-81120460 Msa0526970:three_prime_UTR
TTACCTGTAACTGAAGCTTG+AGG 0.590352 3_4:+81121299 None:intergenic
TTGGACTGGTGTGTACGTAA+AGG 0.597623 3_4:+81122601 None:intergenic
ACTTAAATATGGAGAGTCAT+CGG 0.599086 3_4:+81121946 None:intergenic
ATTCATGTTCATATGATGTG+CGG 0.605271 3_4:+81121121 None:intergenic
CCAATGATAGGAGGAATGAA+AGG 0.605339 3_4:+81120930 None:intergenic
AGGTGCATTGGTATGGCTAG+AGG 0.613493 3_4:+81122093 None:intergenic
AATCAAGGAAAGGATAGCTA+GGG 0.621018 3_4:+81120628 None:intergenic
TCAGAGATGACAAGAAGCAA+TGG 0.621101 3_4:-81122490 Msa0526970:five_prime_UTR
GTTGTGAGCCAATCAAGGAA+AGG 0.623915 3_4:+81120618 None:intergenic
GGAATGAAAATCACTGTGAG+AGG 0.626133 3_4:+81122459 None:intergenic
GAAGAGTTTCCAATGATAGG+AGG 0.626184 3_4:+81120921 None:intergenic
AATCACTGTGAGAGGAACCT+AGG 0.630251 3_4:+81122467 None:intergenic
GTTGATTTGAGAAACACTGC+AGG 0.646249 3_4:+81122274 None:intergenic
TGATTGGCTCACAACATCAA+CGG 0.646597 3_4:-81120610 Msa0526970:three_prime_UTR
TTACCAAAAGAGGTTGGTGT+TGG 0.647408 3_4:+81120891 None:intergenic
TGCCCAAGAATATTATGCAT+AGG 0.648045 3_4:-81120825 Msa0526970:three_prime_UTR
AGGGAAGCCTAAAAGAGTGA+AGG 0.651655 3_4:-81120501 Msa0526970:three_prime_UTR
CACAGAAACAGTACTTGATG+GGG 0.651724 3_4:-81122345 Msa0526970:five_prime_UTR
TGCAACAACATATAAGTGAG+CGG 0.659877 3_4:+81120417 None:intergenic
ATACTATTGTGGGATTGCGA+GGG 0.662640 3_4:-81120520 Msa0526970:three_prime_UTR
GTAACCAGAATCAACCTGGA+AGG 0.666181 3_4:-81120872 Msa0526970:CDS
AAAAGAGGTTGGTGTTGGTG+TGG 0.666327 3_4:+81120896 None:intergenic
AATCAACCTGGAAGGACATG+TGG 0.668906 3_4:-81120864 Msa0526970:CDS
TCATTCAAGACGTAAGACAA+GGG 0.693376 3_4:+81120271 None:intergenic
TTGGGAATTGACATGTGTGG+TGG 0.729000 3_4:+81120957 None:intergenic
GCATTGGGAATTGACATGTG+TGG 0.733578 3_4:+81120954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCATAATTTATCTGAA+AGG - chr3_4:81121128-81121147 Msa0526970:CDS 15.0%
!! AATTCATAATTTATCTGAAA+GGG - chr3_4:81121129-81121148 Msa0526970:CDS 15.0%
!! ATTGTTGCATAGAAAATTTA+AGG + chr3_4:81121528-81121547 None:intergenic 20.0%
!! AACATCATAAAATGATTAAC+TGG + chr3_4:81121648-81121667 None:intergenic 20.0%
!! TTGAATCATTAAAGAAAAGT+AGG - chr3_4:81122130-81122149 Msa0526970:five_prime_UTR 20.0%
!! TGAATCATTAAAGAAAAGTA+GGG - chr3_4:81122131-81122150 Msa0526970:five_prime_UTR 20.0%
!! TATTAAAAATCATTGTCAGA+AGG + chr3_4:81122213-81122232 None:intergenic 20.0%
!!! TGACAATGATTTTTAATACA+AGG - chr3_4:81122215-81122234 Msa0526970:five_prime_UTR 20.0%
! CTTACATCATCACTTAAATA+TGG + chr3_4:81120859-81120878 None:intergenic 25.0%
!! TATTTAAGTGATGATGTAAG+TGG - chr3_4:81120859-81120878 Msa0526970:exon 25.0%
! TACAAACGTAAAATAGCATA+TGG + chr3_4:81120896-81120915 None:intergenic 25.0%
! TGAAGAAACACAAGAAAATA+CGG - chr3_4:81121048-81121067 Msa0526970:CDS 25.0%
! AATAAAGTTCAACTTCAACA+AGG + chr3_4:81121370-81121389 None:intergenic 25.0%
!!! TGCTTTGTTTTTGTTATAGA+TGG - chr3_4:81121426-81121445 Msa0526970:intron 25.0%
! AAAAGAGACATTTGATATGT+TGG - chr3_4:81122033-81122052 Msa0526970:five_prime_UTR 25.0%
! AAAGAGACATTTGATATGTT+GGG - chr3_4:81122034-81122053 Msa0526970:five_prime_UTR 25.0%
! TCTTTAATGATTCAAAAAGC+TGG + chr3_4:81122125-81122144 None:intergenic 25.0%
! CTAGATTTCTTGAAATATGT+TGG + chr3_4:81122492-81122511 None:intergenic 25.0%
AAACAACGTACACAAACTTT+AGG - chr3_4:81120218-81120237 Msa0526970:three_prime_UTR 30.0%
CATGTTGCTAATGAAGTAAA+TGG + chr3_4:81120266-81120285 None:intergenic 30.0%
GTGAGTACTATTCTTACTTT+GGG + chr3_4:81120359-81120378 None:intergenic 30.0%
TGTGAGTACTATTCTTACTT+TGG + chr3_4:81120360-81120379 None:intergenic 30.0%
GGGTGTGAATATCAAAAAAA+TGG - chr3_4:81120466-81120485 Msa0526970:three_prime_UTR 30.0%
!! CTTCTTTTGATTCTTTCTCT+TGG + chr3_4:81120558-81120577 None:intergenic 30.0%
TTCATATGCAGAGTTTGATT+TGG + chr3_4:81120632-81120651 None:intergenic 30.0%
TTTCTCTTTCCTCTAATTCT+AGG + chr3_4:81120721-81120740 None:intergenic 30.0%
ACCAAAGTTTAGATCATTTG+AGG + chr3_4:81120803-81120822 None:intergenic 30.0%
! TGGTGTTAGAAAATCATATG+AGG - chr3_4:81120819-81120838 Msa0526970:three_prime_UTR 30.0%
ACTTAAATATGGAGAGTCAT+CGG + chr3_4:81120848-81120867 None:intergenic 30.0%
ATAATTTATCTGAAAGGGTG+AGG - chr3_4:81121134-81121153 Msa0526970:CDS 30.0%
TCACATACCTTATTGCAATT+TGG + chr3_4:81121312-81121331 None:intergenic 30.0%
ATTCATGTTCATATGATGTG+CGG + chr3_4:81121673-81121692 None:intergenic 30.0%
TATGAATGCACAACATTTGA+TGG - chr3_4:81121710-81121729 Msa0526970:CDS 30.0%
ATGAATGCACAACATTTGAT+GGG - chr3_4:81121711-81121730 Msa0526970:CDS 30.0%
AGCCTATGCATAATATTCTT+GGG + chr3_4:81121971-81121990 None:intergenic 30.0%
! TCTTTTAGCATCATGTACTT+TGG + chr3_4:81122019-81122038 None:intergenic 30.0%
TTAATGATTCAAAAAGCTGG+TGG + chr3_4:81122122-81122141 None:intergenic 30.0%
ACCAACTTTGATACTATTGT+GGG - chr3_4:81122261-81122280 Msa0526970:five_prime_UTR 30.0%
AATTTACCTACCAACAAAAG+AGG - chr3_4:81122459-81122478 Msa0526970:five_prime_UTR 30.0%
! AATTTTCCTCTTTTGTTGGT+AGG + chr3_4:81122468-81122487 None:intergenic 30.0%
! TGGAAATTTTCCTCTTTTGT+TGG + chr3_4:81122472-81122491 None:intergenic 30.0%
! TTGCTTACCAATTTTCAACA+TGG - chr3_4:81122545-81122564 Msa0526970:five_prime_UTR 30.0%
TTGGATACCATGTTGAAAAT+TGG + chr3_4:81122555-81122574 None:intergenic 30.0%
AGTGTAAGTTTGAACATGAA+AGG - chr3_4:81122606-81122625 Msa0526970:five_prime_UTR 30.0%
!! AGTTTGTGTACGTTGTTTCT+AGG + chr3_4:81120216-81120235 None:intergenic 35.0%
AGTACTATTCTTACTTTGGG+AGG + chr3_4:81120356-81120375 None:intergenic 35.0%
TCCTCTCAAAAAACCAAGAT+TGG - chr3_4:81120396-81120415 Msa0526970:three_prime_UTR 35.0%
!!! TCCAATCTTGGTTTTTTGAG+AGG + chr3_4:81120400-81120419 None:intergenic 35.0%
!! GTTTGGTTTGAATCCAATCT+TGG + chr3_4:81120412-81120431 None:intergenic 35.0%
TCACAGAAACAGTACTTGAT+GGG - chr3_4:81120445-81120464 Msa0526970:three_prime_UTR 35.0%
AGCTTCAGAATGTTCATCAA+CGG + chr3_4:81120677-81120696 None:intergenic 35.0%
! TAATTCTAGGTGCATTGGTA+TGG + chr3_4:81120708-81120727 None:intergenic 35.0%
GCCTCAAATGATCTAAACTT+TGG - chr3_4:81120799-81120818 Msa0526970:three_prime_UTR 35.0%
ATCAAGCTTCGTAAGAATGA+TGG + chr3_4:81121007-81121026 None:intergenic 35.0%
AAAGAAAAGCCAGCTCATAA+TGG - chr3_4:81121073-81121092 Msa0526970:CDS 35.0%
GCTCATACCAAATTGCAATA+AGG - chr3_4:81121302-81121321 Msa0526970:intron 35.0%
ACGTTGCAGATTATGTCAAT+GGG - chr3_4:81121606-81121625 Msa0526970:intron 35.0%
TTCATGTTCATATGATGTGC+GGG + chr3_4:81121672-81121691 None:intergenic 35.0%
GGAATAGGAAACTGTATTTG+TGG + chr3_4:81121744-81121763 None:intergenic 35.0%
ACAGTTTCCTATTCCACAAA+TGG - chr3_4:81121749-81121768 Msa0526970:CDS 35.0%
CAGTTTCCTATTCCACAAAT+GGG - chr3_4:81121750-81121769 Msa0526970:CDS 35.0%
! TTGATTCTGGTTACCAAAAG+AGG + chr3_4:81121913-81121932 None:intergenic 35.0%
TGCATAATATTCTTGGGCAT+GGG + chr3_4:81121965-81121984 None:intergenic 35.0%
ATGCATAATATTCTTGGGCA+TGG + chr3_4:81121966-81121985 None:intergenic 35.0%
TGCCCAAGAATATTATGCAT+AGG - chr3_4:81121966-81121985 Msa0526970:five_prime_UTR 35.0%
GAGCCTATGCATAATATTCT+TGG + chr3_4:81121972-81121991 None:intergenic 35.0%
TTCTGTGCAATTAGTGTGTT+TGG - chr3_4:81122090-81122109 Msa0526970:five_prime_UTR 35.0%
AATCAAGGAAAGGATAGCTA+GGG + chr3_4:81122166-81122185 None:intergenic 35.0%
CACCAACTTTGATACTATTG+TGG - chr3_4:81122260-81122279 Msa0526970:five_prime_UTR 35.0%
TCCCACAATAGTATCAAAGT+TGG + chr3_4:81122265-81122284 None:intergenic 35.0%
! TGATGATCCTTCACTCTTTT+AGG + chr3_4:81122300-81122319 None:intergenic 35.0%
AACATATAAGTGAGCGGAAA+AGG + chr3_4:81122371-81122390 None:intergenic 35.0%
TGCAACAACATATAAGTGAG+CGG + chr3_4:81122377-81122396 None:intergenic 35.0%
TCATTCAAGACGTAAGACAA+GGG + chr3_4:81122523-81122542 None:intergenic 35.0%
! GTTTTGTACGTCATTAGACT+TGG + chr3_4:81122574-81122593 None:intergenic 35.0%
!! TGTGTACGTTGTTTCTAGGT+TGG + chr3_4:81120212-81120231 None:intergenic 40.0%
TCAGAGATGACAAGAAGCAA+TGG - chr3_4:81120301-81120320 Msa0526970:three_prime_UTR 40.0%
GGAATGAAAATCACTGTGAG+AGG + chr3_4:81120335-81120354 None:intergenic 40.0%
!! GTGAGTTTGTTGTTGCAGTT+TGG + chr3_4:81120429-81120448 None:intergenic 40.0%
CTCACAGAAACAGTACTTGA+TGG - chr3_4:81120444-81120463 Msa0526970:three_prime_UTR 40.0%
CACAGAAACAGTACTTGATG+GGG - chr3_4:81120446-81120465 Msa0526970:three_prime_UTR 40.0%
!! GTTGATTTGAGAAACACTGC+AGG + chr3_4:81120520-81120539 None:intergenic 40.0%
TCAGAATGTTCATCAACGGT+TGG + chr3_4:81120673-81120692 None:intergenic 40.0%
ACCAATGCACCTAGAATTAG+AGG - chr3_4:81120709-81120728 Msa0526970:three_prime_UTR 40.0%
TCCTCTAATTCTAGGTGCAT+TGG + chr3_4:81120713-81120732 None:intergenic 40.0%
TCAAGTGAGCATACAGAAGA+TGG + chr3_4:81120778-81120797 None:intergenic 40.0%
TTACTATGAAGCACGAACAC+TGG - chr3_4:81120921-81120940 Msa0526970:CDS 40.0%
ATTGAAACTGTGTCTGTGTC+TGG + chr3_4:81120959-81120978 None:intergenic 40.0%
ACTCTGTTTCCATTATGAGC+TGG + chr3_4:81121085-81121104 None:intergenic 40.0%
TACCTGTAACTGAAGCTTGA+GGG + chr3_4:81121494-81121513 None:intergenic 40.0%
TTACCTGTAACTGAAGCTTG+AGG + chr3_4:81121495-81121514 None:intergenic 40.0%
GACGTTGCAGATTATGTCAA+TGG - chr3_4:81121605-81121624 Msa0526970:intron 40.0%
!! CACAACATTTGATGGGTTTC+AGG - chr3_4:81121718-81121737 Msa0526970:CDS 40.0%
AACGACAGAGTTCAGATGTT+TGG - chr3_4:81121795-81121814 Msa0526970:five_prime_UTR 40.0%
TTGACATGTGTGGTGGTAAT+TGG + chr3_4:81121830-81121849 None:intergenic 40.0%
CCAATGATAGGAGGAATGAA+AGG + chr3_4:81121864-81121883 None:intergenic 40.0%
CCTTTCATTCCTCCTATCAT+TGG - chr3_4:81121861-81121880 Msa0526970:five_prime_UTR 40.0%
GAAGAGTTTCCAATGATAGG+AGG + chr3_4:81121873-81121892 None:intergenic 40.0%
GTGGAAGAGTTTCCAATGAT+AGG + chr3_4:81121876-81121895 None:intergenic 40.0%
!! TTACCAAAAGAGGTTGGTGT+TGG + chr3_4:81121903-81121922 None:intergenic 40.0%
! TTCTGGTTACCAAAAGAGGT+TGG + chr3_4:81121909-81121928 None:intergenic 40.0%
! TTTGGTAACCAGAATCAACC+TGG - chr3_4:81121915-81121934 Msa0526970:five_prime_UTR 40.0%
GAATTGCGAAATACAGTAGC+TGG + chr3_4:81122073-81122092 None:intergenic 40.0%
CAATCAAGGAAAGGATAGCT+AGG + chr3_4:81122167-81122186 None:intergenic 40.0%
CTAGCTATCCTTTCCTTGAT+TGG - chr3_4:81122165-81122184 Msa0526970:five_prime_UTR 40.0%
TTGATGTTGTGAGCCAATCA+AGG + chr3_4:81122181-81122200 None:intergenic 40.0%
! TGATTGGCTCACAACATCAA+CGG - chr3_4:81122181-81122200 Msa0526970:five_prime_UTR 40.0%
ATACTATTGTGGGATTGCGA+GGG - chr3_4:81122271-81122290 Msa0526970:five_prime_UTR 40.0%
CTCATTCAAGACGTAAGACA+AGG + chr3_4:81122524-81122543 None:intergenic 40.0%
!! TTGGACTGGTGTGTACGTAA+AGG + chr3_4:81120193-81120212 None:intergenic 45.0%
!! ACGTTGTTTCTAGGTTGGAC+TGG + chr3_4:81120207-81120226 None:intergenic 45.0%
ATGACAAGAAGCAATGGCCT+AGG - chr3_4:81120307-81120326 Msa0526970:three_prime_UTR 45.0%
AATCACTGTGAGAGGAACCT+AGG + chr3_4:81120327-81120346 None:intergenic 45.0%
ACAGAGTCAAGAGAAGCTAC+AGG - chr3_4:81121098-81121117 Msa0526970:CDS 45.0%
CCATCACCCATTTGTGGAAT+AGG + chr3_4:81121759-81121778 None:intergenic 45.0%
CCTATTCCACAAATGGGTGA+TGG - chr3_4:81121756-81121775 Msa0526970:CDS 45.0%
CATGTGTGGTGGTAATTGGT+TGG + chr3_4:81121826-81121845 None:intergenic 45.0%
TTGGGAATTGACATGTGTGG+TGG + chr3_4:81121837-81121856 None:intergenic 45.0%
GCATTGGGAATTGACATGTG+TGG + chr3_4:81121840-81121859 None:intergenic 45.0%
! GGAGGAATGAAAGGTGCATT+GGG + chr3_4:81121855-81121874 None:intergenic 45.0%
! AGGAGGAATGAAAGGTGCAT+TGG + chr3_4:81121856-81121875 None:intergenic 45.0%
!! AAAAGAGGTTGGTGTTGGTG+TGG + chr3_4:81121898-81121917 None:intergenic 45.0%
! ACACCAACACCAACCTCTTT+TGG - chr3_4:81121897-81121916 Msa0526970:five_prime_UTR 45.0%
GTAACCAGAATCAACCTGGA+AGG - chr3_4:81121919-81121938 Msa0526970:five_prime_UTR 45.0%
!! ATGTCCTTCCAGGTTGATTC+TGG + chr3_4:81121926-81121945 None:intergenic 45.0%
AATCAACCTGGAAGGACATG+TGG - chr3_4:81121927-81121946 Msa0526970:five_prime_UTR 45.0%
GTTGTGAGCCAATCAAGGAA+AGG + chr3_4:81122176-81122195 None:intergenic 45.0%
GATACTATTGTGGGATTGCG+AGG - chr3_4:81122270-81122289 Msa0526970:five_prime_UTR 45.0%
AGGGAAGCCTAAAAGAGTGA+AGG - chr3_4:81122290-81122309 Msa0526970:five_prime_UTR 45.0%
AGAATACTCACTCGCACATG+TGG - chr3_4:81122331-81122350 Msa0526970:five_prime_UTR 45.0%
! AGGTGCATTGGTATGGCTAG+AGG + chr3_4:81120701-81120720 None:intergenic 50.0%
GACCCTCAAGCTTCAGTTAC+AGG - chr3_4:81121489-81121508 Msa0526970:intron 50.0%
GTGACACCATCACCCATTTG+TGG + chr3_4:81121765-81121784 None:intergenic 50.0%
!! AGAGGTTGGTGTTGGTGTGG+TGG + chr3_4:81121895-81121914 None:intergenic 55.0%
! GACTAGCCACATGTCCTTCC+AGG + chr3_4:81121936-81121955 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 81120175 81122638 81120175 ID=Msa0526970;Name=Msa0526970
chr3_4 mRNA 81120175 81122638 81120175 ID=Msa0526970-mRNA-1;Parent=Msa0526970;Name=Msa0526970-mRNA-1;_AED=0.01;_eAED=0.01;_QI=859|1|1|1|1|1|4|685|195
chr3_4 exon 81120175 81121198 81120175 ID=Msa0526970-mRNA-1:exon:24466;Parent=Msa0526970-mRNA-1
chr3_4 exon 81121303 81121368 81121303 ID=Msa0526970-mRNA-1:exon:24465;Parent=Msa0526970-mRNA-1
chr3_4 exon 81121490 81121555 81121490 ID=Msa0526970-mRNA-1:exon:24464;Parent=Msa0526970-mRNA-1
chr3_4 exon 81121663 81122638 81121663 ID=Msa0526970-mRNA-1:exon:24463;Parent=Msa0526970-mRNA-1
chr3_4 five_prime_UTR 81121780 81122638 81121780 ID=Msa0526970-mRNA-1:five_prime_utr;Parent=Msa0526970-mRNA-1
chr3_4 CDS 81121663 81121779 81121663 ID=Msa0526970-mRNA-1:cds;Parent=Msa0526970-mRNA-1
chr3_4 CDS 81121490 81121555 81121490 ID=Msa0526970-mRNA-1:cds;Parent=Msa0526970-mRNA-1
chr3_4 CDS 81121303 81121368 81121303 ID=Msa0526970-mRNA-1:cds;Parent=Msa0526970-mRNA-1
chr3_4 CDS 81120860 81121198 81120860 ID=Msa0526970-mRNA-1:cds;Parent=Msa0526970-mRNA-1
chr3_4 three_prime_UTR 81120175 81120859 81120175 ID=Msa0526970-mRNA-1:three_prime_utr;Parent=Msa0526970-mRNA-1
Gene Sequence

>Msa0526970

ATGTTGCAGAATGATGAAGAAACACAAGAAAATACGGTGAAAGAAAAGCCAGCTCATAATGGAAACAGAGTCAAGAGAAGCTACAGGAATGCAAAAATTCATAATTTATCTGAAAGGAAGAGAAGAGATAAAATTAATGAGAAAATACGTGCATTGAAAGAGCTCATACCAAATTGCAATAAGATGGACAAAGCTTCAATGCTTGACGATGCAATCGATTATCTTAAGACCCTCAAGCTTCAGTTACAGATTATGTCAATGGGAAGAGCACTTTGTATGCCAGTTAATCATTTTATGATGTTACCCGCACATCATATGAACATGAATATGAATATGAATATGAATATGAATGCACAACATTTGATGGGTTTCAGGCCACAAATACAGTTTCCTATTCCACAAATGGGTGATGGTGTCACAAACAATAACAACGACAGAGTTCAGATGTTTGGTTTCTCCAACCAATTACCACCACACATGTCAATTCCCAATGCACCTTTCATTCCTCCTATCATTGGAAACTCTTCCACCACACCAACACCAACCTCTTTTGGTAACCAGAATCAACCTGGAAGGACATGTGGCTAG
Protein sequence

>Msa0526970

MLQNDEETQENTVKEKPAHNGNRVKRSYRNAKIHNLSERKRRDKINEKIRALKELIPNCNKMDKASMLDDAIDYLKTLKLQLQIMSMGRALCMPVNHFMMLPAHHMNMNMNMNMNMNAQHLMGFRPQIQFPIPQMGDGVTNNNNDRVQMFGFSNQLPPHMSIPNAPFIPPIIGNSSTTPTPTSFGNQNQPGRTCG*