Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1127740 | XP_003626080.1 | 83.333 | 390 | 18 | 7 | 1 | 360 | 1 | 373 | 0.0 | 604 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1127740 | sp|Q0JNI9|PIL15_ORYSJ | 30.205 | 341 | 159 | 14 | 71 | 334 | 213 | 551 | 7.55e-24 | 106 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1127740 | G7KUN6 | 83.333 | 390 | 18 | 7 | 1 | 360 | 1 | 373 | 0.0 | 604 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa1127740 | TF | bHLH |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1040550 | Msa1127740 | 0.801729 | 7.849850e-49 | -8.615850e-47 |
| Msa1083000 | Msa1127740 | 0.852950 | 3.297710e-61 | -8.615850e-47 |
| Msa0526970 | Msa1127740 | 0.860159 | 2.508127e-63 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1127740 | MtrunA17_Chr7g0270871 | 83.333 | 390 | 18 | 7 | 1 | 360 | 1 | 373 | 0.0 | 604 |
| Msa1127740 | MtrunA17_Chr1g0193321 | 43.137 | 153 | 49 | 4 | 166 | 289 | 409 | 552 | 4.37e-25 | 106 |
| Msa1127740 | MtrunA17_Chr7g0271251 | 36.111 | 216 | 73 | 9 | 166 | 330 | 379 | 580 | 2.58e-21 | 95.5 |
| Msa1127740 | MtrunA17_Chr1g0182791 | 62.500 | 64 | 19 | 1 | 207 | 270 | 334 | 392 | 1.92e-18 | 86.7 |
| Msa1127740 | MtrunA17_Chr3g0095391 | 40.000 | 130 | 70 | 3 | 170 | 296 | 287 | 411 | 1.38e-17 | 84.0 |
| Msa1127740 | MtrunA17_Chr7g0262691 | 55.714 | 70 | 20 | 2 | 207 | 270 | 269 | 333 | 3.95e-15 | 76.3 |
| Msa1127740 | MtrunA17_Chr5g0402031 | 55.932 | 59 | 26 | 0 | 207 | 265 | 122 | 180 | 1.12e-14 | 74.3 |
| Msa1127740 | MtrunA17_Chr1g0153241 | 67.391 | 46 | 15 | 0 | 207 | 252 | 102 | 147 | 3.75e-14 | 71.6 |
| Msa1127740 | MtrunA17_Chr4g0074161 | 57.143 | 49 | 21 | 0 | 208 | 256 | 224 | 272 | 2.89e-13 | 70.5 |
| Msa1127740 | MtrunA17_Chr1g0190731 | 53.061 | 49 | 23 | 0 | 208 | 256 | 211 | 259 | 2.17e-12 | 67.4 |
| Msa1127740 | MtrunA17_Chr1g0207301 | 35.526 | 76 | 42 | 1 | 207 | 282 | 305 | 373 | 8.33e-12 | 65.9 |
| Msa1127740 | MtrunA17_Chr3g0134741 | 38.158 | 76 | 40 | 1 | 207 | 282 | 246 | 314 | 2.32e-11 | 64.3 |
| Msa1127740 | MtrunA17_Chr7g0231461 | 37.895 | 95 | 52 | 2 | 158 | 252 | 272 | 359 | 4.55e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1127740 | AT2G46970.2 | 35.821 | 201 | 93 | 7 | 166 | 336 | 139 | 333 | 2.92e-23 | 100 |
| Msa1127740 | AT2G46970.3 | 35.821 | 201 | 93 | 7 | 166 | 336 | 148 | 342 | 3.90e-23 | 99.8 |
| Msa1127740 | AT2G46970.1 | 35.821 | 201 | 93 | 7 | 166 | 336 | 170 | 364 | 4.08e-23 | 100 |
| Msa1127740 | AT2G20180.7 | 42.105 | 133 | 60 | 4 | 207 | 334 | 224 | 344 | 1.47e-20 | 92.4 |
| Msa1127740 | AT2G20180.1 | 42.105 | 133 | 60 | 4 | 207 | 334 | 224 | 344 | 1.47e-20 | 92.4 |
| Msa1127740 | AT2G20180.5 | 42.105 | 133 | 60 | 4 | 207 | 334 | 224 | 344 | 1.47e-20 | 92.4 |
| Msa1127740 | AT2G20180.4 | 41.667 | 132 | 62 | 3 | 207 | 334 | 295 | 415 | 4.88e-20 | 91.7 |
| Msa1127740 | AT2G20180.6 | 41.667 | 132 | 62 | 3 | 207 | 334 | 295 | 415 | 4.88e-20 | 91.7 |
| Msa1127740 | AT2G20180.2 | 41.667 | 132 | 62 | 3 | 207 | 334 | 295 | 415 | 4.88e-20 | 91.7 |
| Msa1127740 | AT2G20180.3 | 41.667 | 132 | 62 | 3 | 207 | 334 | 295 | 415 | 4.88e-20 | 91.7 |
| Msa1127740 | AT1G09530.3 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT1G09530.2 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT1G09530.5 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT1G09530.1 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT1G09530.4 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT1G09530.6 | 70.909 | 55 | 16 | 0 | 207 | 261 | 354 | 408 | 7.65e-20 | 91.3 |
| Msa1127740 | AT4G28800.1 | 34.118 | 170 | 89 | 7 | 152 | 304 | 198 | 361 | 2.85e-17 | 83.2 |
| Msa1127740 | AT5G67110.1 | 45.882 | 85 | 43 | 2 | 207 | 288 | 104 | 188 | 5.19e-16 | 76.6 |
| Msa1127740 | AT2G20180.8 | 68.889 | 45 | 14 | 0 | 207 | 251 | 295 | 339 | 2.37e-15 | 76.6 |
| Msa1127740 | AT4G28790.1 | 40.196 | 102 | 48 | 3 | 207 | 304 | 288 | 380 | 2.58e-15 | 77.0 |
| Msa1127740 | AT3G59060.1 | 35.484 | 155 | 86 | 6 | 212 | 355 | 272 | 423 | 3.93e-15 | 76.6 |
| Msa1127740 | AT3G59060.3 | 37.984 | 129 | 71 | 4 | 212 | 331 | 272 | 400 | 7.85e-15 | 75.9 |
| Msa1127740 | AT3G59060.2 | 37.984 | 129 | 71 | 4 | 212 | 331 | 272 | 400 | 7.85e-15 | 75.9 |
| Msa1127740 | AT3G59060.4 | 37.984 | 129 | 71 | 4 | 212 | 331 | 272 | 400 | 7.85e-15 | 75.9 |
| Msa1127740 | AT4G36930.1 | 56.667 | 60 | 21 | 1 | 207 | 261 | 208 | 267 | 2.96e-14 | 73.6 |
| Msa1127740 | AT2G24260.2 | 59.184 | 49 | 20 | 0 | 208 | 256 | 53 | 101 | 3.25e-14 | 72.0 |
| Msa1127740 | AT4G28800.2 | 33.133 | 166 | 90 | 6 | 152 | 304 | 210 | 367 | 5.15e-14 | 73.6 |
| Msa1127740 | AT2G24260.1 | 59.184 | 49 | 20 | 0 | 208 | 256 | 156 | 204 | 1.17e-13 | 71.6 |
| Msa1127740 | AT4G30980.1 | 57.143 | 49 | 21 | 0 | 208 | 256 | 148 | 196 | 2.90e-13 | 70.1 |
| Msa1127740 | AT2G43010.2 | 45.455 | 88 | 31 | 2 | 182 | 252 | 226 | 313 | 3.07e-13 | 70.9 |
| Msa1127740 | AT2G43010.1 | 45.455 | 88 | 31 | 2 | 182 | 252 | 226 | 313 | 3.26e-13 | 70.9 |
| Msa1127740 | AT2G43010.4 | 45.455 | 88 | 31 | 2 | 182 | 252 | 226 | 313 | 3.70e-13 | 70.5 |
| Msa1127740 | AT2G43010.3 | 45.455 | 88 | 31 | 2 | 182 | 252 | 233 | 320 | 3.78e-13 | 70.9 |
| Msa1127740 | AT4G30980.2 | 57.143 | 49 | 21 | 0 | 208 | 256 | 196 | 244 | 3.93e-13 | 70.1 |
| Msa1127740 | AT2G43010.5 | 45.455 | 88 | 31 | 2 | 182 | 252 | 268 | 355 | 4.22e-13 | 70.5 |
| Msa1127740 | AT3G62090.1 | 39.062 | 128 | 70 | 5 | 208 | 334 | 183 | 303 | 4.57e-13 | 70.1 |
| Msa1127740 | AT3G62090.2 | 37.415 | 147 | 83 | 6 | 208 | 352 | 200 | 339 | 4.94e-13 | 70.1 |
| Msa1127740 | AT5G58010.1 | 53.061 | 49 | 23 | 0 | 208 | 256 | 117 | 165 | 2.38e-12 | 67.4 |
| Msa1127740 | AT4G00050.2 | 54.902 | 51 | 23 | 0 | 203 | 253 | 118 | 168 | 8.19e-12 | 65.9 |
| Msa1127740 | AT4G00050.1 | 54.902 | 51 | 23 | 0 | 203 | 253 | 220 | 270 | 1.14e-11 | 65.9 |
| Msa1127740 | AT4G00050.3 | 54.902 | 51 | 23 | 0 | 203 | 253 | 220 | 270 | 1.18e-11 | 65.9 |
| Msa1127740 | AT4G28815.2 | 43.056 | 72 | 24 | 2 | 207 | 278 | 85 | 139 | 1.42e-11 | 64.3 |
| Msa1127740 | AT1G02340.2 | 47.143 | 70 | 26 | 2 | 195 | 253 | 115 | 184 | 2.96e-11 | 63.9 |
| Msa1127740 | AT1G02340.1 | 47.143 | 70 | 26 | 2 | 195 | 253 | 122 | 191 | 3.05e-11 | 63.9 |
| Msa1127740 | AT4G28815.1 | 43.056 | 72 | 24 | 2 | 207 | 278 | 161 | 215 | 3.45e-11 | 63.9 |
Find 107 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAACATTTGATGGGTTTC+AGG | 0.156734 | 7_3:+79410069 | Msa1127740:CDS |
| TTTCTCTTTCCTCTAATTCT+AGG | 0.178319 | 7_3:-79409075 | None:intergenic |
| GACCCTCAAGCTTCAGTTAC+AGG | 0.189644 | 7_3:+79409846 | Msa1127740:CDS |
| TCACATACCTTATTGCAATT+TGG | 0.224679 | 7_3:-79409666 | None:intergenic |
| TTGGATACCATGTTGAAAAT+TGG | 0.242044 | 7_3:-79410903 | None:intergenic |
| CTAGCTATCCTTTCCTTGAT+TGG | 0.260539 | 7_3:+79410516 | Msa1127740:three_prime_UTR |
| GAGCCTATGCATAATATTCT+TGG | 0.269072 | 7_3:-79410320 | None:intergenic |
| AGTTTGTGTACGTTGTTTCT+AGG | 0.282978 | 7_3:-79408570 | None:intergenic |
| TTGACATGTGTGGTGGTAAT+TGG | 0.291651 | 7_3:-79410178 | None:intergenic |
| ACACCAACACCAACCTCTTT+TGG | 0.299511 | 7_3:+79410248 | Msa1127740:CDS |
| TTCTGTGCAATTAGTGTGTT+TGG | 0.314866 | 7_3:+79410441 | Msa1127740:three_prime_UTR |
| CCTTTCATTCCTCCTATCAT+TGG | 0.324461 | 7_3:+79410212 | Msa1127740:CDS |
| CATGTTGCTAATGAAGTAAA+TGG | 0.334017 | 7_3:-79408620 | None:intergenic |
| GTTACATCATCACTTAAATA+TGG | 0.340356 | 7_3:-79409213 | None:intergenic |
| ATGTCCTTCCAGGTTGATTC+TGG | 0.340577 | 7_3:-79410274 | None:intergenic |
| GACTAGCCACATGTCCTTCC+AGG | 0.347583 | 7_3:-79410284 | None:intergenic |
| ACCAATGCACCTAGAATTAG+AGG | 0.347789 | 7_3:+79409066 | Msa1127740:CDS |
| CCATCACCCATTTGTGGAAT+AGG | 0.357478 | 7_3:-79410107 | None:intergenic |
| AACATCATAAAATGATTAAC+TGG | 0.360190 | 7_3:-79410002 | None:intergenic |
| TGTGAGTACTATTCTTACTT+TGG | 0.374808 | 7_3:-79408714 | None:intergenic |
| TTCATATGCAGAGTTGGATT+TGG | 0.375202 | 7_3:-79408986 | None:intergenic |
| GTTTGGTTTGAATCCAATCT+TGG | 0.379031 | 7_3:-79408766 | None:intergenic |
| AACGACAGAGTTCAGATGTT+TGG | 0.380475 | 7_3:+79410146 | Msa1127740:CDS |
| TATGAATGCACAACATTTGA+TGG | 0.383363 | 7_3:+79410061 | Msa1127740:CDS |
| GTGAGTACTATTCTTACTTT+GGG | 0.386165 | 7_3:-79408713 | None:intergenic |
| ACAGTTTCCTATTCCACAAA+TGG | 0.388703 | 7_3:+79410100 | Msa1127740:CDS |
| TCCTCTAATTCTAGGTGCAT+TGG | 0.390333 | 7_3:-79409067 | None:intergenic |
| GCTCATACCAAATTGCAATA+AGG | 0.391698 | 7_3:+79409659 | Msa1127740:CDS |
| CTAGATTTCTTGAAATATGT+TGG | 0.391916 | 7_3:-79410840 | None:intergenic |
| TTGAATCATTAAAGAAAAGT+AGG | 0.394350 | 7_3:+79410481 | Msa1127740:three_prime_UTR |
| AACATATAAGTGAGCGGAAA+AGG | 0.400492 | 7_3:-79410719 | None:intergenic |
| GACGTTGCAGATTATGTCAA+TGG | 0.409902 | 7_3:+79409962 | Msa1127740:intron |
| AAAAGAGACATTTGATATGT+TGG | 0.414165 | 7_3:+79410384 | Msa1127740:three_prime_UTR |
| AGCCTATGCATAATATTCTT+GGG | 0.415276 | 7_3:-79410319 | None:intergenic |
| AAACAACGTACACAAACTTT+AGG | 0.416300 | 7_3:+79408575 | Msa1127740:five_prime_UTR |
| ACCAAAGTTTAGATCATTTG+AGG | 0.426290 | 7_3:-79409157 | None:intergenic |
| GTGAGTTTGTTGTTGCAGTT+TGG | 0.430149 | 7_3:-79408783 | None:intergenic |
| AAAGAGACATTTGATATGTT+GGG | 0.434907 | 7_3:+79410385 | Msa1127740:three_prime_UTR |
| GCCTCAAATGATCTAAACTT+TGG | 0.436672 | 7_3:+79409156 | Msa1127740:CDS |
| ACCAACTTTGATACTATTGT+GGG | 0.440078 | 7_3:+79410612 | Msa1127740:three_prime_UTR |
| TTGATTCTGGTTACCAAAAG+AGG | 0.453744 | 7_3:-79410261 | None:intergenic |
| CTCACAGAAACAGTACTTGA+TGG | 0.454187 | 7_3:+79408801 | Msa1127740:CDS |
| TGTGTACGTTGTTTCTAGGT+TGG | 0.455594 | 7_3:-79408566 | None:intergenic |
| ACTTCTTTCATATGCAGAGT+TGG | 0.462349 | 7_3:-79408992 | None:intergenic |
| CACCAACTTTGATACTATTG+TGG | 0.465880 | 7_3:+79410611 | Msa1127740:three_prime_UTR |
| AGGAGGAATGAAAGGTGCAT+TGG | 0.466560 | 7_3:-79410204 | None:intergenic |
| TGAATCATTAAAGAAAAGTA+GGG | 0.467598 | 7_3:+79410482 | Msa1127740:three_prime_UTR |
| GGAGGAATGAAAGGTGCATT+GGG | 0.473309 | 7_3:-79410203 | None:intergenic |
| GTGGAAGAGTTTCCAATGAT+AGG | 0.480699 | 7_3:-79410224 | None:intergenic |
| TACCTGTAACTGAAGCTTGA+GGG | 0.481997 | 7_3:-79409848 | None:intergenic |
| GTGACACCATCACCCATTTG+TGG | 0.484725 | 7_3:-79410113 | None:intergenic |
| CAATCAAGGAAAGGATAGCT+AGG | 0.490800 | 7_3:-79410515 | None:intergenic |
| CAGTTTCCTATTCCACAAAT+GGG | 0.491361 | 7_3:+79410101 | Msa1127740:CDS |
| ATGCATAATATTCTTGGGCA+TGG | 0.495441 | 7_3:-79410314 | None:intergenic |
| GATACTATTGTGGGATTGCG+AGG | 0.504119 | 7_3:+79410621 | Msa1127740:three_prime_UTR |
| AGTGTAAGTTTGAACATGAA+AGG | 0.504506 | 7_3:+79410957 | Msa1127740:three_prime_UTR |
| TAATTCTAGGTGCATTGGTA+TGG | 0.508040 | 7_3:-79409062 | None:intergenic |
| TGAAGAAACACAAGAAAATA+CGG | 0.510993 | 7_3:+79409405 | Msa1127740:CDS |
| AATTTACCTACCAACAAAAG+AGG | 0.512256 | 7_3:+79410810 | Msa1127740:three_prime_UTR |
| TGGTGTTAGAAAATCATATG+AGG | 0.518657 | 7_3:+79409176 | Msa1127740:CDS |
| GAATTGAGAAATACAGTAGC+TGG | 0.519086 | 7_3:-79410421 | None:intergenic |
| TCAAGTGAGCATACAGAAGA+TGG | 0.531560 | 7_3:-79409132 | None:intergenic |
| TCACAGAAACAGTACTTGAT+GGG | 0.539019 | 7_3:+79408802 | Msa1127740:CDS |
| ATGAATGCACAACATTTGAT+GGG | 0.539585 | 7_3:+79410062 | Msa1127740:CDS |
| TCCCACAATAGTATCAAAGT+TGG | 0.540946 | 7_3:-79410613 | None:intergenic |
| ACGTTGCAGATTATGTCAAT+GGG | 0.542904 | 7_3:+79409963 | Msa1127740:intron |
| AGTACTATTCTTACTTTGGG+AGG | 0.546973 | 7_3:-79408710 | None:intergenic |
| GGAATAGGAAACTGTATTTG+TGG | 0.547372 | 7_3:-79410092 | None:intergenic |
| TGCATAATATTCTTGGGCAT+GGG | 0.547979 | 7_3:-79410313 | None:intergenic |
| CATGTGTGGTGGTAATTGGT+TGG | 0.548002 | 7_3:-79410174 | None:intergenic |
| AGAGGTTGGTGTTGGTGTGG+TGG | 0.551444 | 7_3:-79410243 | None:intergenic |
| TTCATGTTCATATGATGTGC+GGG | 0.552429 | 7_3:-79410026 | None:intergenic |
| ATGACAAGAAGCAATGGCCT+AGG | 0.554224 | 7_3:+79408664 | Msa1127740:CDS |
| CCTATTCCACAAATGGGTGA+TGG | 0.561913 | 7_3:+79410107 | Msa1127740:CDS |
| TTGATGTTGTGAGCCAATCA+AGG | 0.563413 | 7_3:-79410529 | None:intergenic |
| TTCTGGTTACCAAAAGAGGT+TGG | 0.567323 | 7_3:-79410257 | None:intergenic |
| TTGGACTTGTGTGTACGTAA+AGG | 0.572746 | 7_3:-79408547 | None:intergenic |
| TCAGAATGTTCATCAACGGT+TGG | 0.575923 | 7_3:-79409027 | None:intergenic |
| AGCTTCAGAATGTTCATCAA+CGG | 0.575933 | 7_3:-79409031 | None:intergenic |
| TTTGGTAACCAGAATCAACC+TGG | 0.577623 | 7_3:+79410266 | Msa1127740:CDS |
| CTCATTCAAGACGTAAGACA+AGG | 0.579237 | 7_3:-79410872 | None:intergenic |
| AGAATACTCACTCGCACATG+TGG | 0.586661 | 7_3:+79410682 | Msa1127740:three_prime_UTR |
| TTACCTGTAACTGAAGCTTG+AGG | 0.590352 | 7_3:-79409849 | None:intergenic |
| ACTTAAATATGGAGAGTCAT+CGG | 0.599086 | 7_3:-79409202 | None:intergenic |
| ATTCATGTTCATATGATGTG+CGG | 0.605271 | 7_3:-79410027 | None:intergenic |
| CCAATGATAGGAGGAATGAA+AGG | 0.605339 | 7_3:-79410212 | None:intergenic |
| AGGTGCATTGGTATGGCTAG+AGG | 0.613493 | 7_3:-79409055 | None:intergenic |
| AATCAAGGAAAGGATAGCTA+GGG | 0.621018 | 7_3:-79410514 | None:intergenic |
| TCAGAGATGACAAGAAGCAA+TGG | 0.621101 | 7_3:+79408658 | Msa1127740:CDS |
| GTTGTGAGCCAATCAAGGAA+AGG | 0.623915 | 7_3:-79410524 | None:intergenic |
| GGAATGAAAATCACTGTGAG+AGG | 0.626133 | 7_3:-79408689 | None:intergenic |
| GAAGAGTTTCCAATGATAGG+AGG | 0.626184 | 7_3:-79410221 | None:intergenic |
| AATCACTGTGAGAGGAACCT+AGG | 0.630251 | 7_3:-79408681 | None:intergenic |
| GTTGATTTGAGAAACACTGC+AGG | 0.646249 | 7_3:-79408874 | None:intergenic |
| TGATTGGCTCACAACATCAA+CGG | 0.646597 | 7_3:+79410532 | Msa1127740:three_prime_UTR |
| TTACCAAAAGAGGTTGGTGT+TGG | 0.647408 | 7_3:-79410251 | None:intergenic |
| TGCCCAAGAATATTATGCAT+AGG | 0.648045 | 7_3:+79410317 | Msa1127740:three_prime_UTR |
| AGGGAAGCCTAAAAGAGTGA+AGG | 0.651655 | 7_3:+79410641 | Msa1127740:three_prime_UTR |
| CACAGAAACAGTACTTGATG+GGG | 0.651724 | 7_3:+79408803 | Msa1127740:CDS |
| TGCAACAACATATAAGTGAG+CGG | 0.659877 | 7_3:-79410725 | None:intergenic |
| ATACTATTGTGGGATTGCGA+GGG | 0.662640 | 7_3:+79410622 | Msa1127740:three_prime_UTR |
| GTAACCAGAATCAACCTGGA+AGG | 0.666181 | 7_3:+79410270 | Msa1127740:CDS |
| AAAAGAGGTTGGTGTTGGTG+TGG | 0.666327 | 7_3:-79410246 | None:intergenic |
| AATCAACCTGGAAGGACATG+TGG | 0.668906 | 7_3:+79410278 | Msa1127740:CDS |
| TCATTCAAGACGTAAGACAA+GGG | 0.693376 | 7_3:-79410871 | None:intergenic |
| TTGGGAATTGACATGTGTGG+TGG | 0.729000 | 7_3:-79410185 | None:intergenic |
| GCATTGGGAATTGACATGTG+TGG | 0.733578 | 7_3:-79410188 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTCATAATTTATCTGAA+AGG | + | chr7_3:79409485-79409504 | Msa1127740:intron | 15.0% |
| !! | AATTCATAATTTATCTGAAA+GGG | + | chr7_3:79409486-79409505 | Msa1127740:intron | 15.0% |
| !! | ATTGTTGCATAGAAAATTTA+AGG | - | chr7_3:79409885-79409904 | None:intergenic | 20.0% |
| !! | AACATCATAAAATGATTAAC+TGG | - | chr7_3:79410005-79410024 | None:intergenic | 20.0% |
| !! | TTGAATCATTAAAGAAAAGT+AGG | + | chr7_3:79410481-79410500 | Msa1127740:three_prime_UTR | 20.0% |
| !! | TGAATCATTAAAGAAAAGTA+GGG | + | chr7_3:79410482-79410501 | Msa1127740:three_prime_UTR | 20.0% |
| !! | TATTAAAAATCATTGTCAGA+AGG | - | chr7_3:79410564-79410583 | None:intergenic | 20.0% |
| !!! | TGACAATGATTTTTAATACA+AGG | + | chr7_3:79410566-79410585 | Msa1127740:three_prime_UTR | 20.0% |
| ! | GTTACATCATCACTTAAATA+TGG | - | chr7_3:79409216-79409235 | None:intergenic | 25.0% |
| !! | TATTTAAGTGATGATGTAAC+TGG | + | chr7_3:79409216-79409235 | Msa1127740:intron | 25.0% |
| ! | TACAAACGTAAAATAGCATA+TGG | - | chr7_3:79409253-79409272 | None:intergenic | 25.0% |
| ! | TGAAGAAACACAAGAAAATA+CGG | + | chr7_3:79409405-79409424 | Msa1127740:CDS | 25.0% |
| ! | AATAAAGTTCAACTTCAACA+AGG | - | chr7_3:79409727-79409746 | None:intergenic | 25.0% |
| !!! | TGCTTTGTTTTTGTTATAGA+TGG | + | chr7_3:79409783-79409802 | Msa1127740:intron | 25.0% |
| ! | AAAAGAGACATTTGATATGT+TGG | + | chr7_3:79410384-79410403 | Msa1127740:three_prime_UTR | 25.0% |
| ! | AAAGAGACATTTGATATGTT+GGG | + | chr7_3:79410385-79410404 | Msa1127740:three_prime_UTR | 25.0% |
| ! | TCTTTAATGATTCAAAAAGC+TGG | - | chr7_3:79410476-79410495 | None:intergenic | 25.0% |
| ! | CTAGATTTCTTGAAATATGT+TGG | - | chr7_3:79410843-79410862 | None:intergenic | 25.0% |
| AAACAACGTACACAAACTTT+AGG | + | chr7_3:79408575-79408594 | Msa1127740:five_prime_UTR | 30.0% | |
| CATGTTGCTAATGAAGTAAA+TGG | - | chr7_3:79408623-79408642 | None:intergenic | 30.0% | |
| GTGAGTACTATTCTTACTTT+GGG | - | chr7_3:79408716-79408735 | None:intergenic | 30.0% | |
| TGTGAGTACTATTCTTACTT+TGG | - | chr7_3:79408717-79408736 | None:intergenic | 30.0% | |
| GGGTGTGAATATCAAAAAAA+TGG | + | chr7_3:79408823-79408842 | Msa1127740:CDS | 30.0% | |
| !! | CTTCTTTTGATTCTTTCTCT+TGG | - | chr7_3:79408915-79408934 | None:intergenic | 30.0% |
| TTTCTCTTTCCTCTAATTCT+AGG | - | chr7_3:79409078-79409097 | None:intergenic | 30.0% | |
| ACCAAAGTTTAGATCATTTG+AGG | - | chr7_3:79409160-79409179 | None:intergenic | 30.0% | |
| ! | TGGTGTTAGAAAATCATATG+AGG | + | chr7_3:79409176-79409195 | Msa1127740:CDS | 30.0% |
| ACTTAAATATGGAGAGTCAT+CGG | - | chr7_3:79409205-79409224 | None:intergenic | 30.0% | |
| ATAATTTATCTGAAAGGGTG+AGG | + | chr7_3:79409491-79409510 | Msa1127740:intron | 30.0% | |
| TCACATACCTTATTGCAATT+TGG | - | chr7_3:79409669-79409688 | None:intergenic | 30.0% | |
| ATTCATGTTCATATGATGTG+CGG | - | chr7_3:79410030-79410049 | None:intergenic | 30.0% | |
| TATGAATGCACAACATTTGA+TGG | + | chr7_3:79410061-79410080 | Msa1127740:CDS | 30.0% | |
| ATGAATGCACAACATTTGAT+GGG | + | chr7_3:79410062-79410081 | Msa1127740:CDS | 30.0% | |
| AGCCTATGCATAATATTCTT+GGG | - | chr7_3:79410322-79410341 | None:intergenic | 30.0% | |
| ! | TCTTTTAGCATCATGTACTT+TGG | - | chr7_3:79410370-79410389 | None:intergenic | 30.0% |
| TTAATGATTCAAAAAGCTGG+TGG | - | chr7_3:79410473-79410492 | None:intergenic | 30.0% | |
| ACCAACTTTGATACTATTGT+GGG | + | chr7_3:79410612-79410631 | Msa1127740:three_prime_UTR | 30.0% | |
| AATTTACCTACCAACAAAAG+AGG | + | chr7_3:79410810-79410829 | Msa1127740:three_prime_UTR | 30.0% | |
| ! | AATTTTCCTCTTTTGTTGGT+AGG | - | chr7_3:79410819-79410838 | None:intergenic | 30.0% |
| ! | TGGAAATTTTCCTCTTTTGT+TGG | - | chr7_3:79410823-79410842 | None:intergenic | 30.0% |
| ! | TTGCTTACCAATTTTCAACA+TGG | + | chr7_3:79410896-79410915 | Msa1127740:three_prime_UTR | 30.0% |
| TTGGATACCATGTTGAAAAT+TGG | - | chr7_3:79410906-79410925 | None:intergenic | 30.0% | |
| AGTGTAAGTTTGAACATGAA+AGG | + | chr7_3:79410957-79410976 | Msa1127740:three_prime_UTR | 30.0% | |
| !! | AGTTTGTGTACGTTGTTTCT+AGG | - | chr7_3:79408573-79408592 | None:intergenic | 35.0% |
| AGTACTATTCTTACTTTGGG+AGG | - | chr7_3:79408713-79408732 | None:intergenic | 35.0% | |
| TCCTCTCAAAAAACCAAGAT+TGG | + | chr7_3:79408753-79408772 | Msa1127740:CDS | 35.0% | |
| !!! | TCCAATCTTGGTTTTTTGAG+AGG | - | chr7_3:79408757-79408776 | None:intergenic | 35.0% |
| !! | GTTTGGTTTGAATCCAATCT+TGG | - | chr7_3:79408769-79408788 | None:intergenic | 35.0% |
| TCACAGAAACAGTACTTGAT+GGG | + | chr7_3:79408802-79408821 | Msa1127740:CDS | 35.0% | |
| TTCATATGCAGAGTTGGATT+TGG | - | chr7_3:79408989-79409008 | None:intergenic | 35.0% | |
| ACTTCTTTCATATGCAGAGT+TGG | - | chr7_3:79408995-79409014 | None:intergenic | 35.0% | |
| AGCTTCAGAATGTTCATCAA+CGG | - | chr7_3:79409034-79409053 | None:intergenic | 35.0% | |
| ! | TAATTCTAGGTGCATTGGTA+TGG | - | chr7_3:79409065-79409084 | None:intergenic | 35.0% |
| GCCTCAAATGATCTAAACTT+TGG | + | chr7_3:79409156-79409175 | Msa1127740:CDS | 35.0% | |
| ATCAAGCTTCGTAAGAATGA+TGG | - | chr7_3:79409364-79409383 | None:intergenic | 35.0% | |
| AAAGAAAAGCCAGCTCATAA+TGG | + | chr7_3:79409430-79409449 | Msa1127740:intron | 35.0% | |
| GCTCATACCAAATTGCAATA+AGG | + | chr7_3:79409659-79409678 | Msa1127740:CDS | 35.0% | |
| ACGTTGCAGATTATGTCAAT+GGG | + | chr7_3:79409963-79409982 | Msa1127740:intron | 35.0% | |
| TTCATGTTCATATGATGTGC+GGG | - | chr7_3:79410029-79410048 | None:intergenic | 35.0% | |
| GGAATAGGAAACTGTATTTG+TGG | - | chr7_3:79410095-79410114 | None:intergenic | 35.0% | |
| ACAGTTTCCTATTCCACAAA+TGG | + | chr7_3:79410100-79410119 | Msa1127740:CDS | 35.0% | |
| CAGTTTCCTATTCCACAAAT+GGG | + | chr7_3:79410101-79410120 | Msa1127740:CDS | 35.0% | |
| ! | TTGATTCTGGTTACCAAAAG+AGG | - | chr7_3:79410264-79410283 | None:intergenic | 35.0% |
| TGCATAATATTCTTGGGCAT+GGG | - | chr7_3:79410316-79410335 | None:intergenic | 35.0% | |
| ATGCATAATATTCTTGGGCA+TGG | - | chr7_3:79410317-79410336 | None:intergenic | 35.0% | |
| TGCCCAAGAATATTATGCAT+AGG | + | chr7_3:79410317-79410336 | Msa1127740:three_prime_UTR | 35.0% | |
| GAGCCTATGCATAATATTCT+TGG | - | chr7_3:79410323-79410342 | None:intergenic | 35.0% | |
| GAATTGAGAAATACAGTAGC+TGG | - | chr7_3:79410424-79410443 | None:intergenic | 35.0% | |
| TTCTGTGCAATTAGTGTGTT+TGG | + | chr7_3:79410441-79410460 | Msa1127740:three_prime_UTR | 35.0% | |
| AATCAAGGAAAGGATAGCTA+GGG | - | chr7_3:79410517-79410536 | None:intergenic | 35.0% | |
| CACCAACTTTGATACTATTG+TGG | + | chr7_3:79410611-79410630 | Msa1127740:three_prime_UTR | 35.0% | |
| TCCCACAATAGTATCAAAGT+TGG | - | chr7_3:79410616-79410635 | None:intergenic | 35.0% | |
| ! | TGATGATCCTTCACTCTTTT+AGG | - | chr7_3:79410651-79410670 | None:intergenic | 35.0% |
| AACATATAAGTGAGCGGAAA+AGG | - | chr7_3:79410722-79410741 | None:intergenic | 35.0% | |
| TGCAACAACATATAAGTGAG+CGG | - | chr7_3:79410728-79410747 | None:intergenic | 35.0% | |
| TCATTCAAGACGTAAGACAA+GGG | - | chr7_3:79410874-79410893 | None:intergenic | 35.0% | |
| ! | GTTTTGTACGTCATTAGACT+TGG | - | chr7_3:79410925-79410944 | None:intergenic | 35.0% |
| TTGGACTTGTGTGTACGTAA+AGG | - | chr7_3:79408550-79408569 | None:intergenic | 40.0% | |
| !! | TGTGTACGTTGTTTCTAGGT+TGG | - | chr7_3:79408569-79408588 | None:intergenic | 40.0% |
| TCAGAGATGACAAGAAGCAA+TGG | + | chr7_3:79408658-79408677 | Msa1127740:CDS | 40.0% | |
| GGAATGAAAATCACTGTGAG+AGG | - | chr7_3:79408692-79408711 | None:intergenic | 40.0% | |
| !! | GTGAGTTTGTTGTTGCAGTT+TGG | - | chr7_3:79408786-79408805 | None:intergenic | 40.0% |
| CTCACAGAAACAGTACTTGA+TGG | + | chr7_3:79408801-79408820 | Msa1127740:CDS | 40.0% | |
| CACAGAAACAGTACTTGATG+GGG | + | chr7_3:79408803-79408822 | Msa1127740:CDS | 40.0% | |
| !! | GTTGATTTGAGAAACACTGC+AGG | - | chr7_3:79408877-79408896 | None:intergenic | 40.0% |
| TCAGAATGTTCATCAACGGT+TGG | - | chr7_3:79409030-79409049 | None:intergenic | 40.0% | |
| ACCAATGCACCTAGAATTAG+AGG | + | chr7_3:79409066-79409085 | Msa1127740:CDS | 40.0% | |
| TCCTCTAATTCTAGGTGCAT+TGG | - | chr7_3:79409070-79409089 | None:intergenic | 40.0% | |
| TCAAGTGAGCATACAGAAGA+TGG | - | chr7_3:79409135-79409154 | None:intergenic | 40.0% | |
| TTACTATGAAGCACGAACAC+TGG | + | chr7_3:79409278-79409297 | Msa1127740:intron | 40.0% | |
| ATTGAAACTGTGTCTGTGTC+TGG | - | chr7_3:79409316-79409335 | None:intergenic | 40.0% | |
| ACTCAGTTTCCATTATGAGC+TGG | - | chr7_3:79409442-79409461 | None:intergenic | 40.0% | |
| TACCTGTAACTGAAGCTTGA+GGG | - | chr7_3:79409851-79409870 | None:intergenic | 40.0% | |
| TTACCTGTAACTGAAGCTTG+AGG | - | chr7_3:79409852-79409871 | None:intergenic | 40.0% | |
| GACGTTGCAGATTATGTCAA+TGG | + | chr7_3:79409962-79409981 | Msa1127740:intron | 40.0% | |
| !! | CACAACATTTGATGGGTTTC+AGG | + | chr7_3:79410069-79410088 | Msa1127740:CDS | 40.0% |
| AACGACAGAGTTCAGATGTT+TGG | + | chr7_3:79410146-79410165 | Msa1127740:CDS | 40.0% | |
| TTGACATGTGTGGTGGTAAT+TGG | - | chr7_3:79410181-79410200 | None:intergenic | 40.0% | |
| CCAATGATAGGAGGAATGAA+AGG | - | chr7_3:79410215-79410234 | None:intergenic | 40.0% | |
| CCTTTCATTCCTCCTATCAT+TGG | + | chr7_3:79410212-79410231 | Msa1127740:CDS | 40.0% | |
| GAAGAGTTTCCAATGATAGG+AGG | - | chr7_3:79410224-79410243 | None:intergenic | 40.0% | |
| GTGGAAGAGTTTCCAATGAT+AGG | - | chr7_3:79410227-79410246 | None:intergenic | 40.0% | |
| !! | TTACCAAAAGAGGTTGGTGT+TGG | - | chr7_3:79410254-79410273 | None:intergenic | 40.0% |
| ! | TTCTGGTTACCAAAAGAGGT+TGG | - | chr7_3:79410260-79410279 | None:intergenic | 40.0% |
| ! | TTTGGTAACCAGAATCAACC+TGG | + | chr7_3:79410266-79410285 | Msa1127740:CDS | 40.0% |
| CAATCAAGGAAAGGATAGCT+AGG | - | chr7_3:79410518-79410537 | None:intergenic | 40.0% | |
| CTAGCTATCCTTTCCTTGAT+TGG | + | chr7_3:79410516-79410535 | Msa1127740:three_prime_UTR | 40.0% | |
| TTGATGTTGTGAGCCAATCA+AGG | - | chr7_3:79410532-79410551 | None:intergenic | 40.0% | |
| ! | TGATTGGCTCACAACATCAA+CGG | + | chr7_3:79410532-79410551 | Msa1127740:three_prime_UTR | 40.0% |
| ATACTATTGTGGGATTGCGA+GGG | + | chr7_3:79410622-79410641 | Msa1127740:three_prime_UTR | 40.0% | |
| CTCATTCAAGACGTAAGACA+AGG | - | chr7_3:79410875-79410894 | None:intergenic | 40.0% | |
| ATGACAAGAAGCAATGGCCT+AGG | + | chr7_3:79408664-79408683 | Msa1127740:CDS | 45.0% | |
| AATCACTGTGAGAGGAACCT+AGG | - | chr7_3:79408684-79408703 | None:intergenic | 45.0% | |
| ACTGAGTCAAGAGAAGCTAC+AGG | + | chr7_3:79409455-79409474 | Msa1127740:intron | 45.0% | |
| CCATCACCCATTTGTGGAAT+AGG | - | chr7_3:79410110-79410129 | None:intergenic | 45.0% | |
| CCTATTCCACAAATGGGTGA+TGG | + | chr7_3:79410107-79410126 | Msa1127740:CDS | 45.0% | |
| CATGTGTGGTGGTAATTGGT+TGG | - | chr7_3:79410177-79410196 | None:intergenic | 45.0% | |
| TTGGGAATTGACATGTGTGG+TGG | - | chr7_3:79410188-79410207 | None:intergenic | 45.0% | |
| GCATTGGGAATTGACATGTG+TGG | - | chr7_3:79410191-79410210 | None:intergenic | 45.0% | |
| ! | GGAGGAATGAAAGGTGCATT+GGG | - | chr7_3:79410206-79410225 | None:intergenic | 45.0% |
| ! | AGGAGGAATGAAAGGTGCAT+TGG | - | chr7_3:79410207-79410226 | None:intergenic | 45.0% |
| !! | AAAAGAGGTTGGTGTTGGTG+TGG | - | chr7_3:79410249-79410268 | None:intergenic | 45.0% |
| ! | ACACCAACACCAACCTCTTT+TGG | + | chr7_3:79410248-79410267 | Msa1127740:CDS | 45.0% |
| GTAACCAGAATCAACCTGGA+AGG | + | chr7_3:79410270-79410289 | Msa1127740:CDS | 45.0% | |
| !! | ATGTCCTTCCAGGTTGATTC+TGG | - | chr7_3:79410277-79410296 | None:intergenic | 45.0% |
| AATCAACCTGGAAGGACATG+TGG | + | chr7_3:79410278-79410297 | Msa1127740:CDS | 45.0% | |
| GTTGTGAGCCAATCAAGGAA+AGG | - | chr7_3:79410527-79410546 | None:intergenic | 45.0% | |
| GATACTATTGTGGGATTGCG+AGG | + | chr7_3:79410621-79410640 | Msa1127740:three_prime_UTR | 45.0% | |
| AGGGAAGCCTAAAAGAGTGA+AGG | + | chr7_3:79410641-79410660 | Msa1127740:three_prime_UTR | 45.0% | |
| AGAATACTCACTCGCACATG+TGG | + | chr7_3:79410682-79410701 | Msa1127740:three_prime_UTR | 45.0% | |
| ! | AGGTGCATTGGTATGGCTAG+AGG | - | chr7_3:79409058-79409077 | None:intergenic | 50.0% |
| GACCCTCAAGCTTCAGTTAC+AGG | + | chr7_3:79409846-79409865 | Msa1127740:CDS | 50.0% | |
| GTGACACCATCACCCATTTG+TGG | - | chr7_3:79410116-79410135 | None:intergenic | 50.0% | |
| !! | AGAGGTTGGTGTTGGTGTGG+TGG | - | chr7_3:79410246-79410265 | None:intergenic | 55.0% |
| ! | GACTAGCCACATGTCCTTCC+AGG | - | chr7_3:79410287-79410306 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 79408532 | 79410989 | 79408532 | ID=Msa1127740;Name=Msa1127740 |
| chr7_3 | mRNA | 79408532 | 79410989 | 79408532 | ID=Msa1127740-mRNA-1;Parent=Msa1127740;Name=Msa1127740-mRNA-1;_AED=0.06;_eAED=0.06;_QI=108|0.75|0.8|1|1|1|5|685|360 |
| chr7_3 | exon | 79408532 | 79409230 | 79408532 | ID=Msa1127740-mRNA-1:exon:22464;Parent=Msa1127740-mRNA-1 |
| chr7_3 | exon | 79409400 | 79409426 | 79409400 | ID=Msa1127740-mRNA-1:exon:22465;Parent=Msa1127740-mRNA-1 |
| chr7_3 | exon | 79409615 | 79409680 | 79409615 | ID=Msa1127740-mRNA-1:exon:22466;Parent=Msa1127740-mRNA-1 |
| chr7_3 | exon | 79409802 | 79409867 | 79409802 | ID=Msa1127740-mRNA-1:exon:22467;Parent=Msa1127740-mRNA-1 |
| chr7_3 | exon | 79409972 | 79410989 | 79409972 | ID=Msa1127740-mRNA-1:exon:22468;Parent=Msa1127740-mRNA-1 |
| chr7_3 | five_prime_UTR | 79408532 | 79408639 | 79408532 | ID=Msa1127740-mRNA-1:five_prime_utr;Parent=Msa1127740-mRNA-1 |
| chr7_3 | CDS | 79408640 | 79409230 | 79408640 | ID=Msa1127740-mRNA-1:cds;Parent=Msa1127740-mRNA-1 |
| chr7_3 | CDS | 79409400 | 79409426 | 79409400 | ID=Msa1127740-mRNA-1:cds;Parent=Msa1127740-mRNA-1 |
| chr7_3 | CDS | 79409615 | 79409680 | 79409615 | ID=Msa1127740-mRNA-1:cds;Parent=Msa1127740-mRNA-1 |
| chr7_3 | CDS | 79409802 | 79409867 | 79409802 | ID=Msa1127740-mRNA-1:cds;Parent=Msa1127740-mRNA-1 |
| chr7_3 | CDS | 79409972 | 79410304 | 79409972 | ID=Msa1127740-mRNA-1:cds;Parent=Msa1127740-mRNA-1 |
| chr7_3 | three_prime_UTR | 79410305 | 79410989 | 79410305 | ID=Msa1127740-mRNA-1:three_prime_utr;Parent=Msa1127740-mRNA-1 |
| Gene Sequence |
| Protein sequence |
