AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0532510

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532510 MtrunA17_Chr4g0005921 90.995 422 8 1 31 422 81 502 0.0 737
Msa0532510 MtrunA17_Chr3g0104061 67.426 439 106 4 14 422 70 501 0.0 591
Msa0532510 MtrunA17_Chr5g0429511 65.848 448 114 6 7 422 63 503 0.0 584
Msa0532510 MtrunA17_Chr5g0436461 59.122 433 143 5 19 420 68 497 0.0 528
Msa0532510 MtrunA17_Chr5g0404341 54.464 448 166 5 7 422 70 511 3.46e-170 489
Msa0532510 MtrunA17_Chr5g0404361 55.792 423 153 4 31 422 137 556 2.17e-164 476
Msa0532510 MtrunA17_Chr7g0238981 55.402 435 151 5 31 422 104 538 2.91e-164 475
Msa0532510 MtrunA17_Chr7g0237491 51.204 457 175 6 10 422 73 525 8.65e-155 451
Msa0532510 MtrunA17_Chr7g0237441 50.670 448 177 5 19 422 78 525 1.63e-153 447
Msa0532510 MtrunA17_Chr7g0238351 49.782 458 181 6 10 422 73 526 6.05e-151 441
Msa0532510 MtrunA17_Chr7g0238941 48.000 450 193 6 14 422 74 523 8.28e-143 420
Msa0532510 MtrunA17_Chr4g0031901 47.672 451 194 6 10 422 84 530 9.50e-142 417
Msa0532510 MtrunA17_Chr3g0114211 47.442 430 180 8 31 420 90 513 1.68e-133 395
Msa0532510 MtrunA17_Chr5g0437681 45.622 434 188 7 31 420 81 510 8.17e-132 391
Msa0532510 MtrunA17_Chr5g0431581 46.512 430 186 7 31 422 82 505 2.55e-122 367
Msa0532510 MtrunA17_Chr4g0008521 41.055 436 212 5 31 422 83 517 8.90e-107 327
Msa0532510 MtrunA17_Chr8g0346581 40.323 434 209 8 31 422 91 516 1.48e-106 327
Msa0532510 MtrunA17_Chr7g0240971 39.735 453 227 6 14 422 65 515 2.23e-104 321
Msa0532510 MtrunA17_Chr7g0240991 37.004 454 239 9 14 422 65 516 4.03e-99 307
Msa0532510 MtrunA17_Chr2g0278811 36.184 456 225 6 31 420 95 550 7.11e-95 297
Msa0532510 MtrunA17_Chr5g0436431 57.870 216 87 3 206 420 1 213 1.52e-83 256
Msa0532510 MtrunA17_Chr3g0133861 43.165 278 138 10 157 420 12 283 4.59e-57 191
Msa0532510 MtrunA17_Chr3g0133871 54.098 122 56 0 31 152 89 210 2.23e-41 146
Msa0532510 MtrunA17_Chr3g0118321 28.604 444 238 15 31 416 84 506 6.43e-34 133
Msa0532510 MtrunA17_Chr3g0143671 26.959 434 264 13 12 419 69 475 2.92e-29 120
Msa0532510 MtrunA17_Chr8g0374271 28.475 446 254 18 7 419 62 475 5.26e-29 119
Msa0532510 MtrunA17_Chr5g0402831 26.905 420 241 18 31 419 88 472 2.99e-28 117
Msa0532510 MtrunA17_Chr4g0038671 26.498 434 265 14 12 419 69 474 1.05e-27 115
Msa0532510 MtrunA17_Chr8g0374261 26.211 454 259 16 2 419 60 473 3.10e-27 114
Msa0532510 MtrunA17_Chr3g0118331 26.364 440 253 13 31 416 83 505 1.62e-26 112
Msa0532510 MtrunA17_Chr1g0157341 26.037 434 268 13 12 419 68 474 1.20e-25 109
Msa0532510 MtrunA17_Chr4g0024861 26.147 436 265 17 12 419 66 472 2.64e-24 105
Msa0532510 MtrunA17_Chr3g0079321 24.330 448 245 18 32 422 87 497 2.72e-23 102
Msa0532510 MtrunA17_Chr2g0322441 27.169 438 258 19 12 419 69 475 1.06e-22 100
Msa0532510 MtrunA17_Chr4g0056511 24.161 447 246 17 32 422 85 494 7.28e-22 98.2
Msa0532510 MtrunA17_Chr3g0118351 31.058 293 160 11 154 419 3 280 3.56e-20 91.3
Msa0532510 MtrunA17_Chr4g0024641 25.412 425 254 21 25 419 4 395 1.40e-19 91.7
Msa0532510 MtrunA17_Chr4g0075071 28.188 447 247 18 8 419 69 476 2.99e-19 90.1
Msa0532510 MtrunA17_Chr8g0382691 38.400 125 73 2 299 419 390 514 5.54e-18 86.3
Msa0532510 MtrunA17_Chr8g0382691 26.291 213 107 7 31 197 92 300 1.16e-14 76.3
Msa0532510 MtrunA17_Chr1g0197311 35.052 97 60 3 21 115 101 196 1.27e-11 64.7
Msa0532510 MtrunA17_Chr3g0118341 36.667 90 54 3 31 119 84 171 9.59e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0532510 AT2G38080.1 67.489 446 110 4 7 422 63 503 0.0 615
Msa0532510 AT5G01190.1 67.612 423 105 3 31 422 82 503 0.0 566
Msa0532510 AT5G01190.2 67.612 423 105 3 31 422 68 489 0.0 565
Msa0532510 AT5G58910.2 58.499 453 145 7 7 422 66 512 3.06e-178 510
Msa0532510 AT5G58910.3 58.499 453 145 7 7 422 65 511 3.85e-178 510
Msa0532510 AT5G58910.1 59.382 421 133 5 38 422 50 468 9.43e-172 492
Msa0532510 AT5G03260.1 52.455 448 172 7 7 422 64 502 2.16e-157 457
Msa0532510 AT5G60020.2 48.247 485 194 9 7 438 61 541 1.33e-148 434
Msa0532510 AT5G60020.3 48.927 466 184 8 7 422 29 490 2.84e-147 431
Msa0532510 AT5G60020.1 48.927 466 184 8 7 422 61 522 5.53e-147 431
Msa0532510 AT2G29130.1 46.171 457 201 9 7 422 66 518 3.72e-137 405
Msa0532510 AT5G05390.1 47.907 430 179 7 31 420 84 508 1.62e-133 396
Msa0532510 AT2G30210.1 45.354 452 196 8 14 422 72 515 7.74e-129 384
Msa0532510 AT2G40370.1 44.719 445 184 9 31 420 86 523 2.43e-125 375
Msa0532510 AT1G18140.1 42.308 468 212 10 7 422 65 526 1.71e-123 371
Msa0532510 AT5G07130.1 46.847 444 174 10 31 422 81 514 5.01e-123 369
Msa0532510 AT5G07130.2 46.241 439 174 10 36 422 1 429 1.02e-119 358
Msa0532510 AT5G07130.3 46.241 439 174 10 36 422 1 429 1.02e-119 358
Msa0532510 AT5G09360.1 41.920 427 204 7 31 420 93 512 1.02e-110 338
Msa0532510 AT2G46570.2 40.323 434 211 7 30 422 3 429 2.33e-110 334
Msa0532510 AT2G46570.1 40.185 433 211 7 31 422 89 514 1.63e-108 332
Msa0532510 AT3G09220.1 41.228 456 218 9 9 422 65 512 1.54e-104 322
Msa0532510 AT5G01040.1 39.686 446 220 9 19 422 73 511 9.46e-102 315
Msa0532510 AT5G01050.1 39.908 436 211 8 31 422 85 513 5.50e-101 313
Msa0532510 AT3G09220.2 41.627 418 198 8 47 422 2 415 2.03e-98 303
Msa0532510 AT5G48100.1 39.352 432 216 11 31 420 79 506 4.14e-94 295
Msa0532510 AT4G39830.1 29.213 445 245 13 31 419 94 524 4.14e-39 149
Msa0532510 AT4G39830.2 28.571 441 245 13 35 419 3 429 3.89e-36 139
Msa0532510 AT4G39830.3 28.539 438 243 13 38 419 4 427 1.59e-35 137
Msa0532510 AT5G21105.3 28.668 443 240 14 31 416 96 519 2.51e-34 135
Msa0532510 AT5G21105.1 28.668 443 240 14 31 416 96 519 4.68e-34 134
Msa0532510 AT5G21100.1 28.027 446 238 15 31 417 82 503 2.26e-33 132
Msa0532510 AT4G39830.4 27.500 440 238 12 35 419 3 416 4.55e-33 130
Msa0532510 AT4G37160.1 25.532 423 247 12 31 419 88 476 2.77e-28 117
Msa0532510 AT2G23630.2 27.512 418 242 13 32 419 93 479 3.07e-27 114
Msa0532510 AT2G23630.1 27.512 418 242 13 32 419 88 474 3.57e-27 114
Msa0532510 AT4G22010.1 27.232 448 260 17 4 419 59 472 7.42e-26 110
Msa0532510 AT1G75790.1 26.606 436 266 16 13 419 67 477 8.09e-26 110
Msa0532510 AT4G25240.2 26.622 447 234 17 32 422 87 495 8.30e-26 110
Msa0532510 AT4G25240.1 26.622 447 234 17 32 422 87 495 8.30e-26 110
Msa0532510 AT5G66920.1 26.185 443 254 16 12 419 76 480 9.71e-26 110
Msa0532510 AT4G28090.1 25.513 439 267 15 12 419 67 476 5.74e-25 107
Msa0532510 AT1G21860.1 24.885 434 271 15 12 419 68 472 5.43e-24 104
Msa0532510 AT5G48450.2 26.966 445 265 17 12 422 68 486 7.01e-24 104
Msa0532510 AT5G48450.1 26.966 445 265 17 12 422 68 486 8.40e-24 104
Msa0532510 AT4G37160.2 24.557 395 231 11 31 391 88 449 2.90e-23 102
Msa0532510 AT4G38420.3 24.832 447 263 14 12 419 69 481 3.86e-23 102
Msa0532510 AT5G51480.1 23.884 448 246 15 32 422 86 495 5.04e-23 102
Msa0532510 AT4G38420.1 24.832 447 263 14 12 419 69 481 5.07e-23 102
Msa0532510 AT4G12420.1 23.146 445 255 15 31 422 82 492 7.67e-21 95.5
Msa0532510 AT4G12420.2 23.146 445 255 15 31 422 82 492 7.67e-21 95.5
Msa0532510 AT5G21105.2 29.811 265 168 5 156 416 78 328 2.63e-19 89.7
Msa0532510 AT4G38420.2 24.155 414 252 12 12 391 69 454 3.72e-19 89.7
Msa0532510 AT1G55570.1 23.934 422 261 16 32 419 87 482 1.67e-16 82.0
Msa0532510 AT1G55560.1 23.206 418 268 15 32 419 84 478 3.38e-16 81.3

Find 84 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCACAATTGTTTCAATT+TGG 0.198155 4_1:+6620389 None:intergenic
TCAACAAGGTTAAACTTCTT+TGG 0.219638 4_1:+6619698 None:intergenic
TTTGGCATGCACATGTTAAT+TGG 0.227901 4_1:-6621318 Msa0532510:CDS
AACATTAACTGGGTATTTCT+TGG 0.229766 4_1:+6620129 None:intergenic
AACCATCCTGTTCATCTTCA+TGG 0.254799 4_1:-6619776 Msa0532510:CDS
TCAGATGCTCATACCATAAA+TGG 0.255242 4_1:-6620867 Msa0532510:CDS
ACATACAATTTCACCATTAC+TGG 0.266011 4_1:-6621358 Msa0532510:CDS
TGTCCCAGGAAGACCATTTA+TGG 0.286955 4_1:+6620854 None:intergenic
GCCAGATGATGAATTGGTTT+TGG 0.300374 4_1:-6621227 Msa0532510:CDS
TTTCTTTGGTAAAATAACTA+TGG 0.303777 4_1:+6621273 None:intergenic
GTCAAAGAGGAACACTTCTT+TGG 0.310116 4_1:-6621336 Msa0532510:CDS
GGATAGGGAACACCTTTCTT+TGG 0.316191 4_1:+6621259 None:intergenic
CCAATTCATCATCTGGCTTT+GGG 0.320192 4_1:+6621233 None:intergenic
ACCAATTCATCATCTGGCTT+TGG 0.321201 4_1:+6621232 None:intergenic
CCCAAAGCCAGATGATGAAT+TGG 0.321450 4_1:-6621233 Msa0532510:CDS
TTATAAATATGAGGTGGATT+TGG 0.329000 4_1:+6619917 None:intergenic
GCAACAGGTGCATCCATAAA+AGG 0.347626 4_1:+6620295 None:intergenic
GGTTTCAATTTCTTTGTTGT+TGG 0.348108 4_1:-6619755 Msa0532510:CDS
CAGCTTGTTATGCAAGATAC+TGG 0.352988 4_1:-6619815 Msa0532510:CDS
ATTTATAATTTCACTGGTGC+TGG 0.376600 4_1:-6619902 Msa0532510:CDS
TAACTATGTCCTGGTTGAAT+TGG 0.376716 4_1:+6621382 None:intergenic
ACAGCTACATTACATTATTC+AGG 0.378100 4_1:-6620253 Msa0532510:CDS
TTGTTGCATATTTCCAGCTT+TGG 0.394093 4_1:+6618429 None:intergenic
GGAGGTGTAGTTAGAAATGT+TGG 0.405928 4_1:+6620211 None:intergenic
CCAATAGTGTTCCTTTCAAC+AGG 0.406648 4_1:+6619674 None:intergenic
CATACCATAAATGGTCTTCC+TGG 0.407581 4_1:-6620858 Msa0532510:CDS
CTTCTACTACTGTGAGTTTG+TGG 0.408210 4_1:+6620437 None:intergenic
AATACCTTGTGTAGAACAAT+TGG 0.408385 4_1:+6620827 None:intergenic
TTTGTTGTTGGTAAAGGAGT+TGG 0.409860 4_1:-6619743 Msa0532510:CDS
TTGAAACCATGAAGATGAAC+AGG 0.413774 4_1:+6619770 None:intergenic
AATTTCTTTGTTGTTGGTAA+AGG 0.416507 4_1:-6619749 Msa0532510:CDS
GAACAATTGGCCACTGTCCC+AGG 0.420964 4_1:+6620840 None:intergenic
ACCAAAACCAATTCATCATC+TGG 0.422160 4_1:+6621226 None:intergenic
AACTATGTCCTGGTTGAATT+GGG 0.424212 4_1:+6621383 None:intergenic
ATCAATGAAGCACTAAAATC+AGG 0.432147 4_1:-6620906 Msa0532510:CDS
GTTAAATTGTCTACAGCAAC+AGG 0.433150 4_1:+6620280 None:intergenic
GTCATTATTATCATGTTGGC+AGG 0.434547 4_1:-6621465 Msa0532510:CDS
ATGTTGGCAGGCATGGTATT+AGG 0.435889 4_1:-6621453 Msa0532510:CDS
CCTTCATGTAAAGCTGGAAA+TGG 0.453329 4_1:-6620049 Msa0532510:CDS
TATTATCATGTTGGCAGGCA+TGG 0.460505 4_1:-6621460 Msa0532510:CDS
CCTCATATTTATAATTTCAC+TGG 0.464423 4_1:-6619908 Msa0532510:CDS
ACTTTGCACTCGACCAAAGC+TGG 0.472524 4_1:-6618442 Msa0532510:CDS
CCTTGTCCTTCATGTAAAGC+TGG 0.472570 4_1:-6620055 Msa0532510:CDS
GGTATTAGGCAATTGAGAAC+AGG 0.474093 4_1:-6621439 Msa0532510:CDS
TAAATATGAGGTGGATTTGG+TGG 0.477812 4_1:+6619920 None:intergenic
GAGGTGTAGTTAGAAATGTT+GGG 0.483750 4_1:+6620212 None:intergenic
TTGAGAACAGGTTGGGCTGA+TGG 0.489336 4_1:-6621427 Msa0532510:CDS
TAGGCAATTGAGAACAGGTT+GGG 0.493895 4_1:-6621434 Msa0532510:CDS
TGTTCCTCTTTGACCAGTAA+TGG 0.494174 4_1:+6621345 None:intergenic
AGATGAACAGGATGGTTCTC+AGG 0.496558 4_1:+6619782 None:intergenic
CTTTGGTCGAGTGCAAAGTT+AGG 0.497718 4_1:+6618446 None:intergenic
CCATTTCCAGCTTTACATGA+AGG 0.504039 4_1:+6620049 None:intergenic
TCATCATCTGGCTTTGGGAA+AGG 0.504275 4_1:+6621238 None:intergenic
GTATGAGCATCTGAAACATT+TGG 0.509634 4_1:+6620876 None:intergenic
ATCTGGCTTTGGGAAAGGAT+AGG 0.518016 4_1:+6621243 None:intergenic
TTGAGTCATTATTATCATGT+TGG 0.522115 4_1:-6621469 Msa0532510:CDS
ACAATTGTTTCAATTTGGAA+TGG 0.524020 4_1:+6620394 None:intergenic
TCTGGCTTTGGGAAAGGATA+GGG 0.535295 4_1:+6621244 None:intergenic
AGTACATTTGTTGTTTGACC+AGG 0.537617 4_1:+6620364 None:intergenic
CCAGTGAAATTATAAATATG+AGG 0.540266 4_1:+6619908 None:intergenic
TTCACCATTACTGGTCAAAG+AGG 0.543070 4_1:-6621349 Msa0532510:CDS
AACACTATTGGTGTACCAGC+TGG 0.553147 4_1:-6619662 Msa0532510:CDS
CCAGCTTTACATGAAGGACA+AGG 0.558261 4_1:+6620055 None:intergenic
CCTGTTGAAAGGAACACTAT+TGG 0.559502 4_1:-6619674 Msa0532510:CDS
AAATGGTCTTCCTGGGACAG+TGG 0.566886 4_1:-6620850 Msa0532510:CDS
ATACCATAAATGGTCTTCCT+GGG 0.571091 4_1:-6620857 Msa0532510:CDS
ACCTTGTTGATCCTGTTGAA+AGG 0.571542 4_1:-6619685 Msa0532510:CDS
TGGCTAAGATCAACTGTCCA+TGG 0.575480 4_1:-6621298 Msa0532510:CDS
TGAAGCACTAAAATCAGGAA+TGG 0.590307 4_1:-6620901 Msa0532510:CDS
ATTGCAACCCAACCACCAGC+TGG 0.593002 4_1:+6619647 None:intergenic
GAAACAATTGTGATAGCACC+TGG 0.597555 4_1:-6620382 Msa0532510:CDS
GCTTGTAGTAAAGCTGTAGT+TGG 0.610290 4_1:+6619983 None:intergenic
TTAGGCAATTGAGAACAGGT+TGG 0.615713 4_1:-6621435 Msa0532510:CDS
TGAATAATGTAATGTAGCTG+TGG 0.621640 4_1:+6620255 None:intergenic
GTGAAATTATAAATATGAGG+TGG 0.624519 4_1:+6619911 None:intergenic
TCCTTTCAACAGGATCAACA+AGG 0.625266 4_1:+6619684 None:intergenic
GGGCATTGTGTGATATATGC+AGG 0.628669 4_1:+6621403 None:intergenic
TTGTATGTATAACTATGTCC+TGG 0.632359 4_1:+6621373 None:intergenic
GTAAAATAACTATGGCACCA+TGG 0.647215 4_1:+6621281 None:intergenic
ACACAATGCCCAATTCAACC+AGG 0.651958 4_1:-6621391 Msa0532510:CDS
AGGTGTAGTTAGAAATGTTG+GGG 0.665714 4_1:+6620213 None:intergenic
AACCATGAAGATGAACAGGA+TGG 0.677352 4_1:+6619774 None:intergenic
GTGGCCAATTGTTCTACACA+AGG 0.704099 4_1:-6620831 Msa0532510:intron
ACTATTGGTGTACCAGCTGG+TGG 0.711640 4_1:-6619659 Msa0532510:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTAAAATGTTTTGTTTAT+TGG - chr4_1:6618924-6618943 Msa0532510:intron 10.0%
!!! TAAAAGCAAATAATTTTTTA+GGG + chr4_1:6619211-6619230 None:intergenic 10.0%
!!! TAAATTTTCTCATTTATATA+TGG - chr4_1:6620982-6621001 Msa0532510:intron 10.0%
!! ATTCTAATTCATGTTTATAA+TGG - chr4_1:6618969-6618988 Msa0532510:intron 15.0%
!!! CTAAAAGCAAATAATTTTTT+AGG + chr4_1:6619212-6619231 None:intergenic 15.0%
!!! AATTTTTTAAACTCACTTAA+AGG - chr4_1:6619790-6619809 Msa0532510:CDS 15.0%
!!! AGTAAAGATAAAAGATATTT+TGG - chr4_1:6621275-6621294 Msa0532510:CDS 15.0%
!!! TGATTAGATCTTTTTCTATA+TGG + chr4_1:6618432-6618451 None:intergenic 20.0%
!! TTTCTTTGGTAAAATAACTA+TGG + chr4_1:6618680-6618699 None:intergenic 20.0%
!!! ATAGTTATTTTACCAAAGAA+AGG - chr4_1:6618679-6618698 Msa0532510:intron 20.0%
!!! TTAATGAAAGTTTTTGTGTT+TGG + chr4_1:6618761-6618780 None:intergenic 20.0%
!! TGATGATTATGATTGATTAA+CGG - chr4_1:6618868-6618887 Msa0532510:intron 20.0%
!! AAACTCTATAAATATCATCA+AGG - chr4_1:6619167-6619186 Msa0532510:intron 20.0%
!! AACTCTATAAATATCATCAA+GGG - chr4_1:6619168-6619187 Msa0532510:intron 20.0%
!!! TGAATATAATCTAAGCTTTT+TGG - chr4_1:6619369-6619388 Msa0532510:intron 20.0%
!!! CTTTTTGGTTTTTTCTTATT+TGG - chr4_1:6619384-6619403 Msa0532510:intron 20.0%
!!! CTAAATATTTTCCAGATTTT+TGG + chr4_1:6619624-6619643 None:intergenic 20.0%
!!! TTTTTAAAGGAACATTAACT+GGG + chr4_1:6619834-6619853 None:intergenic 20.0%
!!! ATTTTTAAAGGAACATTAAC+TGG + chr4_1:6619835-6619854 None:intergenic 20.0%
!!! AGTGAATGATCAATTTTTAA+AGG + chr4_1:6619847-6619866 None:intergenic 20.0%
!!! TTTTTTTTACTGTTGGATTA+GGG - chr4_1:6619866-6619885 Msa0532510:CDS 20.0%
!!! NNNNNNTTTTTTTAAGGGGT+TGG - chr4_1:6620433-6620452 Msa0532510:CDS 20.0%
!!! TGTTGATACTCAATATTTTA+GGG + chr4_1:6620523-6620542 None:intergenic 20.0%
!!! TTGTTGATACTCAATATTTT+AGG + chr4_1:6620524-6620543 None:intergenic 20.0%
!! ATTGAGTATCAACAATAAAA+CGG - chr4_1:6620529-6620548 Msa0532510:intron 20.0%
!! ATCATTGTAACAAAAATAAG+AGG + chr4_1:6620615-6620634 None:intergenic 20.0%
!!! ACATAATCTAGTTGTATTTT+TGG - chr4_1:6620807-6620826 Msa0532510:intron 20.0%
!! TAAAAGTCTAGTTACATATA+AGG + chr4_1:6620964-6620983 None:intergenic 20.0%
!! TACAAATTTCATGTAAGTAA+TGG + chr4_1:6621481-6621500 None:intergenic 20.0%
!! ATCTTTTTCTATATGGTCAA+AGG + chr4_1:6618425-6618444 None:intergenic 25.0%
! TTGAGTCATTATTATCATGT+TGG - chr4_1:6618481-6618500 Msa0532510:intron 25.0%
!! ATTGATGATTCTTAGTAAGT+AGG + chr4_1:6619455-6619474 None:intergenic 25.0%
! ACAATTGTTTCAATTTGGAA+TGG + chr4_1:6619559-6619578 None:intergenic 25.0%
! CTATCACAATTGTTTCAATT+TGG + chr4_1:6619564-6619583 None:intergenic 25.0%
! TTAAAAGCAAACCAAAAATC+TGG - chr4_1:6619610-6619629 Msa0532510:intron 25.0%
!!! CATTCACTTTTTTTTACTGT+TGG - chr4_1:6619859-6619878 Msa0532510:CDS 25.0%
!!! CTTTTTTTTACTGTTGGATT+AGG - chr4_1:6619865-6619884 Msa0532510:CDS 25.0%
!!! TTTTTTTACTGTTGGATTAG+GGG - chr4_1:6619867-6619886 Msa0532510:CDS 25.0%
!! TTATAAATATGAGGTGGATT+TGG + chr4_1:6620036-6620055 None:intergenic 25.0%
! GTGAAATTATAAATATGAGG+TGG + chr4_1:6620042-6620061 None:intergenic 25.0%
! CCAGTGAAATTATAAATATG+AGG + chr4_1:6620045-6620064 None:intergenic 25.0%
! CCTCATATTTATAATTTCAC+TGG - chr4_1:6620042-6620061 Msa0532510:CDS 25.0%
!!! AATTTCTTTGTTGTTGGTAA+AGG - chr4_1:6620201-6620220 Msa0532510:CDS 25.0%
! GTATAATTAATATTGAGTGC+AGG + chr4_1:6620839-6620858 None:intergenic 25.0%
!! CTCAAAGAATGAACTAATTT+TGG + chr4_1:6620861-6620880 None:intergenic 25.0%
! CAAAATTAGTTCATTCTTTG+AGG - chr4_1:6620859-6620878 Msa0532510:CDS 25.0%
! ATAAGCTCTCATCATTATTA+CGG + chr4_1:6621052-6621071 None:intergenic 25.0%
TTGTATGTATAACTATGTCC+TGG + chr4_1:6618580-6618599 None:intergenic 30.0%
ACATACAATTTCACCATTAC+TGG - chr4_1:6618592-6618611 Msa0532510:intron 30.0%
!!! GATGAATTGGTTTTGGTATT+AGG - chr4_1:6618730-6618749 Msa0532510:intron 30.0%
GGTCTTTCAATAGCATAAAT+TGG - chr4_1:6618945-6618964 Msa0532510:intron 30.0%
GGTGATGATTGAAAAATTGT+AGG - chr4_1:6618990-6619009 Msa0532510:intron 30.0%
ATTGAAAAATTGTAGGAGAG+TGG - chr4_1:6618997-6619016 Msa0532510:intron 30.0%
! ATCAATGAAGCACTAAAATC+AGG - chr4_1:6619044-6619063 Msa0532510:intron 30.0%
AATACCTTGTGTAGAACAAT+TGG + chr4_1:6619126-6619145 None:intergenic 30.0%
TCATCAGCTTCTTAAAATCA+CGG + chr4_1:6619305-6619324 None:intergenic 30.0%
! GAACTTTTCTTCAAAATAGC+TGG - chr4_1:6619490-6619509 Msa0532510:intron 30.0%
TGAATAATGTAATGTAGCTG+TGG + chr4_1:6619698-6619717 None:intergenic 30.0%
ACAGCTACATTACATTATTC+AGG - chr4_1:6619697-6619716 Msa0532510:CDS 30.0%
!! ATTTGTGTAGCATTTTTAGG+TGG + chr4_1:6619763-6619782 None:intergenic 30.0%
! GCAATTTGTGTAGCATTTTT+AGG + chr4_1:6619766-6619785 None:intergenic 30.0%
AACATTAACTGGGTATTTCT+TGG + chr4_1:6619824-6619843 None:intergenic 30.0%
ATTTATAATTTCACTGGTGC+TGG - chr4_1:6620048-6620067 Msa0532510:CDS 30.0%
!! CTTGTAGTGTTCAAGTTTTT+AGG + chr4_1:6620075-6620094 None:intergenic 30.0%
AAAAACTTGAACACTACAAG+TGG - chr4_1:6620075-6620094 Msa0532510:CDS 30.0%
! GGTTTCAATTTCTTTGTTGT+TGG - chr4_1:6620195-6620214 Msa0532510:CDS 30.0%
TCAACAAGGTTAAACTTCTT+TGG + chr4_1:6620255-6620274 None:intergenic 30.0%
AGAATATGTTGTCTAACCAA+TGG - chr4_1:6620668-6620687 Msa0532510:intron 30.0%
AAGCAATTGTCACAGTTAAT+GGG + chr4_1:6620780-6620799 None:intergenic 30.0%
AAAGCAATTGTCACAGTTAA+TGG + chr4_1:6620781-6620800 None:intergenic 30.0%
TAATTAATATTGAGTGCAGG+TGG + chr4_1:6620836-6620855 None:intergenic 30.0%
ATTATTAGAATGTGCATGTG+TGG - chr4_1:6620887-6620906 Msa0532510:CDS 30.0%
CTATAATCAGCACATAAAGT+TGG + chr4_1:6620935-6620954 None:intergenic 30.0%
!! ACTTTATGTGCTGATTATAG+AGG - chr4_1:6620935-6620954 Msa0532510:CDS 30.0%
GTCATTATTATCATGTTGGC+AGG - chr4_1:6618485-6618504 Msa0532510:intron 35.0%
AACTATGTCCTGGTTGAATT+GGG + chr4_1:6618570-6618589 None:intergenic 35.0%
TAACTATGTCCTGGTTGAAT+TGG + chr4_1:6618571-6618590 None:intergenic 35.0%
TTTGGCATGCACATGTTAAT+TGG - chr4_1:6618632-6618651 Msa0532510:intron 35.0%
GTAAAATAACTATGGCACCA+TGG + chr4_1:6618672-6618691 None:intergenic 35.0%
ACCAAAACCAATTCATCATC+TGG + chr4_1:6618727-6618746 None:intergenic 35.0%
! TGAAGCACTAAAATCAGGAA+TGG - chr4_1:6619049-6619068 Msa0532510:intron 35.0%
! GTATGAGCATCTGAAACATT+TGG + chr4_1:6619077-6619096 None:intergenic 35.0%
TCAGATGCTCATACCATAAA+TGG - chr4_1:6619083-6619102 Msa0532510:intron 35.0%
ATACCATAAATGGTCTTCCT+GGG - chr4_1:6619093-6619112 Msa0532510:intron 35.0%
AGATGTTTACAAACTACCTG+TGG - chr4_1:6619417-6619436 Msa0532510:intron 35.0%
!! AGTACATTTGTTGTTTGACC+AGG + chr4_1:6619589-6619608 None:intergenic 35.0%
GTTAAATTGTCTACAGCAAC+AGG + chr4_1:6619673-6619692 None:intergenic 35.0%
AGGTGTAGTTAGAAATGTTG+GGG + chr4_1:6619740-6619759 None:intergenic 35.0%
GAGGTGTAGTTAGAAATGTT+GGG + chr4_1:6619741-6619760 None:intergenic 35.0%
! TAAATATGAGGTGGATTTGG+TGG + chr4_1:6620033-6620052 None:intergenic 35.0%
TTGAAACCATGAAGATGAAC+AGG + chr4_1:6620183-6620202 None:intergenic 35.0%
!! TTTGTTGTTGGTAAAGGAGT+TGG - chr4_1:6620207-6620226 Msa0532510:CDS 35.0%
TATGTTGTCTAACCAATGGA+TGG - chr4_1:6620672-6620691 Msa0532510:intron 35.0%
ACCAATGGATGGTAATATTG+TGG - chr4_1:6620683-6620702 Msa0532510:intron 35.0%
ACCACAATATTACCATCCAT+TGG + chr4_1:6620687-6620706 None:intergenic 35.0%
TTGTTGCATATTTCCAGCTT+TGG + chr4_1:6621524-6621543 None:intergenic 35.0%
TATTATCATGTTGGCAGGCA+TGG - chr4_1:6618490-6618509 Msa0532510:intron 40.0%
GGTATTAGGCAATTGAGAAC+AGG - chr4_1:6618511-6618530 Msa0532510:intron 40.0%
TTAGGCAATTGAGAACAGGT+TGG - chr4_1:6618515-6618534 Msa0532510:intron 40.0%
TAGGCAATTGAGAACAGGTT+GGG - chr4_1:6618516-6618535 Msa0532510:intron 40.0%
TTCACCATTACTGGTCAAAG+AGG - chr4_1:6618601-6618620 Msa0532510:intron 40.0%
TGTTCCTCTTTGACCAGTAA+TGG + chr4_1:6618608-6618627 None:intergenic 40.0%
GTCAAAGAGGAACACTTCTT+TGG - chr4_1:6618614-6618633 Msa0532510:intron 40.0%
!! CCAATTCATCATCTGGCTTT+GGG + chr4_1:6618720-6618739 None:intergenic 40.0%
! ACCAATTCATCATCTGGCTT+TGG + chr4_1:6618721-6618740 None:intergenic 40.0%
!! GCCAGATGATGAATTGGTTT+TGG - chr4_1:6618723-6618742 Msa0532510:intron 40.0%
GAAAAATTGTAGGAGAGTGG+TGG - chr4_1:6619000-6619019 Msa0532510:intron 40.0%
CATACCATAAATGGTCTTCC+TGG - chr4_1:6619092-6619111 Msa0532510:intron 40.0%
TATCAAACGCCACTTCAAAC+AGG + chr4_1:6619243-6619262 None:intergenic 40.0%
CACGGCAGATTTACATCAAA+AGG + chr4_1:6619287-6619306 None:intergenic 40.0%
TAGAGCTTCTGCGTTTAAAG+TGG - chr4_1:6619326-6619345 Msa0532510:intron 40.0%
CTTCTACTACTGTGAGTTTG+TGG + chr4_1:6619516-6619535 None:intergenic 40.0%
GAAACAATTGTGATAGCACC+TGG - chr4_1:6619568-6619587 Msa0532510:intron 40.0%
! AGTAGCAGCTTCACCTTTTA+TGG - chr4_1:6619642-6619661 Msa0532510:intron 40.0%
! GGAGGTGTAGTTAGAAATGT+TGG + chr4_1:6619742-6619761 None:intergenic 40.0%
!!! TGTGTAGCATTTTTAGGTGG+AGG + chr4_1:6619760-6619779 None:intergenic 40.0%
CCATTTCCAGCTTTACATGA+AGG + chr4_1:6619904-6619923 None:intergenic 40.0%
CCTTCATGTAAAGCTGGAAA+TGG - chr4_1:6619901-6619920 Msa0532510:CDS 40.0%
GCTTGTAGTAAAGCTGTAGT+TGG + chr4_1:6619970-6619989 None:intergenic 40.0%
!! CAGCTTGTTATGCAAGATAC+TGG - chr4_1:6620135-6620154 Msa0532510:CDS 40.0%
AACCATCCTGTTCATCTTCA+TGG - chr4_1:6620174-6620193 Msa0532510:CDS 40.0%
AACCATGAAGATGAACAGGA+TGG + chr4_1:6620179-6620198 None:intergenic 40.0%
ACCTTGTTGATCCTGTTGAA+AGG - chr4_1:6620265-6620284 Msa0532510:CDS 40.0%
TCCTTTCAACAGGATCAACA+AGG + chr4_1:6620269-6620288 None:intergenic 40.0%
CCAATAGTGTTCCTTTCAAC+AGG + chr4_1:6620279-6620298 None:intergenic 40.0%
CCTGTTGAAAGGAACACTAT+TGG - chr4_1:6620276-6620295 Msa0532510:CDS 40.0%
TTAATATTGAGTGCAGGTGG+TGG + chr4_1:6620833-6620852 None:intergenic 40.0%
TTAGAATGTGCATGTGTGGT+TGG - chr4_1:6620891-6620910 Msa0532510:CDS 40.0%
ATGTTGGCAGGCATGGTATT+AGG - chr4_1:6618497-6618516 Msa0532510:intron 45.0%
! GGGCATTGTGTGATATATGC+AGG + chr4_1:6618550-6618569 None:intergenic 45.0%
ACACAATGCCCAATTCAACC+AGG - chr4_1:6618559-6618578 Msa0532510:intron 45.0%
TGGCTAAGATCAACTGTCCA+TGG - chr4_1:6618652-6618671 Msa0532510:intron 45.0%
GGATAGGGAACACCTTTCTT+TGG + chr4_1:6618694-6618713 None:intergenic 45.0%
!! TCTGGCTTTGGGAAAGGATA+GGG + chr4_1:6618709-6618728 None:intergenic 45.0%
!! ATCTGGCTTTGGGAAAGGAT+AGG + chr4_1:6618710-6618729 None:intergenic 45.0%
!! TCATCATCTGGCTTTGGGAA+AGG + chr4_1:6618715-6618734 None:intergenic 45.0%
CCCAAAGCCAGATGATGAAT+TGG - chr4_1:6618717-6618736 Msa0532510:intron 45.0%
TGTCCCAGGAAGACCATTTA+TGG + chr4_1:6619099-6619118 None:intergenic 45.0%
GTGGCCAATTGTTCTACACA+AGG - chr4_1:6619119-6619138 Msa0532510:intron 45.0%
GTCTTAGTGCCTGTTTGAAG+TGG - chr4_1:6619231-6619250 Msa0532510:intron 45.0%
TACAAACTACCTGTGGAGAG+TGG - chr4_1:6619424-6619443 Msa0532510:intron 45.0%
GCAACAGGTGCATCCATAAA+AGG + chr4_1:6619658-6619677 None:intergenic 45.0%
CCAGCTTTACATGAAGGACA+AGG + chr4_1:6619898-6619917 None:intergenic 45.0%
CCTTGTCCTTCATGTAAAGC+TGG - chr4_1:6619895-6619914 Msa0532510:CDS 45.0%
AGATGAACAGGATGGTTCTC+AGG + chr4_1:6620171-6620190 None:intergenic 45.0%
AACACTATTGGTGTACCAGC+TGG - chr4_1:6620288-6620307 Msa0532510:CDS 45.0%
TATCACTGTATCATCCTCCC+TGG - chr4_1:6620729-6620748 Msa0532510:intron 45.0%
ACAGTTAATGGGAAGTTCCC+GGG + chr4_1:6620769-6620788 None:intergenic 45.0%
CACAGTTAATGGGAAGTTCC+CGG + chr4_1:6620770-6620789 None:intergenic 45.0%
CTTTGGTCGAGTGCAAAGTT+AGG + chr4_1:6621507-6621526 None:intergenic 45.0%
!!! NNNNNNNNNNTTTTTTTAAG+GGG - chr4_1:6620429-6620448 Msa0532510:CDS 5.0%
! TTGAGAACAGGTTGGGCTGA+TGG - chr4_1:6618523-6618542 Msa0532510:intron 50.0%
AAATGGTCTTCCTGGGACAG+TGG - chr4_1:6619100-6619119 Msa0532510:intron 50.0%
! TAGGTTTTGCCACTCTCCAC+AGG + chr4_1:6619436-6619455 None:intergenic 50.0%
ACTATTGGTGTACCAGCTGG+TGG - chr4_1:6620291-6620310 Msa0532510:CDS 50.0%
ATCCTCCCTGGCATAAAGAG+TGG - chr4_1:6620741-6620760 Msa0532510:intron 50.0%
TCCTCCCTGGCATAAAGAGT+GGG - chr4_1:6620742-6620761 Msa0532510:intron 50.0%
ACTTTGCACTCGACCAAAGC+TGG - chr4_1:6621508-6621527 Msa0532510:CDS 50.0%
GAACAATTGGCCACTGTCCC+AGG + chr4_1:6619113-6619132 None:intergenic 55.0%
TTGGTGTACCAGCTGGTGGT+TGG - chr4_1:6620295-6620314 Msa0532510:CDS 55.0%
TGGTGTACCAGCTGGTGGTT+GGG - chr4_1:6620296-6620315 Msa0532510:CDS 55.0%
ATTGCAACCCAACCACCAGC+TGG + chr4_1:6620306-6620325 None:intergenic 55.0%
ACCCACTCTTTATGCCAGGG+AGG + chr4_1:6620746-6620765 None:intergenic 55.0%
GGGACCCACTCTTTATGCCA+GGG + chr4_1:6620749-6620768 None:intergenic 55.0%
CTGGCATAAAGAGTGGGTCC+CGG - chr4_1:6620748-6620767 Msa0532510:intron 55.0%
TGGCATAAAGAGTGGGTCCC+GGG - chr4_1:6620749-6620768 Msa0532510:intron 55.0%
CGGGACCCACTCTTTATGCC+AGG + chr4_1:6620750-6620769 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 6618418 6621554 6618418 ID=Msa0532510;Name=Msa0532510
chr4_1 mRNA 6618418 6621554 6618418 ID=Msa0532510-mRNA-1;Parent=Msa0532510;Name=Msa0532510-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|0.75|1|1|4|0|439
chr4_1 exon 6621221 6621554 6621221 ID=Msa0532510-mRNA-1:exon:2377;Parent=Msa0532510-mRNA-1
chr4_1 exon 6620832 6620960 6620832 ID=Msa0532510-mRNA-1:exon:2376;Parent=Msa0532510-mRNA-1
chr4_1 exon 6619660 6620463 6619660 ID=Msa0532510-mRNA-1:exon:2375;Parent=Msa0532510-mRNA-1
chr4_1 exon 6618418 6618470 6618418 ID=Msa0532510-mRNA-1:exon:2374;Parent=Msa0532510-mRNA-1
chr4_1 CDS 6621221 6621554 6621221 ID=Msa0532510-mRNA-1:cds;Parent=Msa0532510-mRNA-1
chr4_1 CDS 6620832 6620960 6620832 ID=Msa0532510-mRNA-1:cds;Parent=Msa0532510-mRNA-1
chr4_1 CDS 6619660 6620463 6619660 ID=Msa0532510-mRNA-1:cds;Parent=Msa0532510-mRNA-1
chr4_1 CDS 6618418 6618470 6618418 ID=Msa0532510-mRNA-1:cds;Parent=Msa0532510-mRNA-1
Gene Sequence

>Msa0532510

TTGACCTTTGACCATATAGAAAAAGATCTAATCAATATAGTTTTGAATTATTTCATTTCTAATTTGAGTCATTATTATCATGTTGGCAGGCATGGTATTAGGCAATTGAGAACAGGTTGGGCTGATGGACCTGCATATATCACACAATGCCCAATTCAACCAGGACATAGTTATACATACAATTTCACCATTACTGGTCAAAGAGGAACACTTCTTTGGCATGCACATGTTAATTGGCTAAGATCAACTGTCCATGGTGCCATAGTTATTTTACCAAAGAAAGGTGTTCCCTATCCTTTCCCAAAGCCAGATGATGAATTGGTTTTGGTATTAGGAGAGTGGTGGAAATCTGATACTGAAGCTGTTATCAATGAAGCACTAAAATCAGGAATGGCACCAAATGTTTCAGATGCTCATACCATAAATGGTCTTCCTGGGACAGTGGCCAATTGTTCTACACAAGCTGGCCACAAACTCACAGTAGTAGAAGTTGATGCAACATACACAAAACCATTCCAAATTGAAACAATTGTGATAGCACCTGGTCAAACAACAAATGTACTATTAAAAGCAAACCAAAAATCTGGAAAATATTTAGTAGCAGCTTCACCTTTTATGGATGCACCTGTTGCTGTAGACAATTTAACAGCCACAGCTACATTACATTATTCAGGAACTACTCTTGCAAACACCCCAACATTTCTAACTACACCTCCACCTAAAAATGCTACACAAATTGCTAATAATTTTTTAAACTCACTTAAAGGACTTAATTCCAAGAAATACCCAGTTAATGTTCCTTTAAAAATTGATCATTCACTTTTTTTTACTGTTGGATTAGGGGTTAATCCTTGTCCTTCATGTAAAGCTGGAAATGGTAGTAGAGTTGTAGCTGCTATTAATAATGTTACATTTGTTATGCCAACTACAGCTTTACTACAAGCACATTATTTCAACATTAAAAATGTTTTTACTACAGATTTTCCACCAAATCCACCTCATATTTATAATTTCACTGGTGCTGGACCTAAAAACTTGAACACTACAAGTGGTACAAAGTTATATAAGTTATCATTTAATGATACTGTTCAGCTTGTTATGCAAGATACTGGAATCATTGCACCTGAGAACCATCCTGTTCATCTTCATGGTTTCAATTTCTTTGTTGTTGGTAAAGGAGTTGGAAATTATGATAGTAAAAATGATCCAAAGAAGTTTAACCTTGTTGATCCTGTTGAAAGGAACACTATTGGTGTACCAGCTGGTGTGCCTAACTTTGCACTCGACCAAAGCTGGAAATATGCAACAAATAAGAAGTAG
Protein sequence

>Msa0532510

LTFDHIEKDLINIVLNYFISNLSHYYHVGRHGIRQLRTGWADGPAYITQCPIQPGHSYTYNFTITGQRGTLLWHAHVNWLRSTVHGAIVILPKKGVPYPFPKPDDELVLVLGEWWKSDTEAVINEALKSGMAPNVSDAHTINGLPGTVANCSTQAGHKLTVVEVDATYTKPFQIETIVIAPGQTTNVLLKANQKSGKYLVAASPFMDAPVAVDNLTATATLHYSGTTLANTPTFLTTPPPKNATQIANNFLNSLKGLNSKKYPVNVPLKIDHSLFFTVGLGVNPCPSCKAGNGSRVVAAINNVTFVMPTTALLQAHYFNIKNVFTTDFPPNPPHIYNFTGAGPKNLNTTSGTKLYKLSFNDTVQLVMQDTGIIAPENHPVHLHGFNFFVVGKGVGNYDSKNDPKKFNLVDPVERNTIGVPAGVPNFALDQSWKYATNKK*