AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0589360

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589360 MtrunA17_Chr4g0005921 98.025 557 11 0 1 557 1 557 0.0 1124
Msa0589360 MtrunA17_Chr5g0429511 72.809 559 146 4 2 557 3 558 0.0 857
Msa0589360 MtrunA17_Chr3g0104061 74.583 539 134 2 19 557 21 556 0.0 847
Msa0589360 MtrunA17_Chr5g0436461 66.487 558 182 4 1 557 1 554 0.0 795
Msa0589360 MtrunA17_Chr5g0404341 60.323 557 218 2 2 557 12 566 0.0 719
Msa0589360 MtrunA17_Chr5g0404361 60.107 559 216 4 3 557 56 611 0.0 711
Msa0589360 MtrunA17_Chr7g0238981 58.874 586 212 6 1 557 8 593 0.0 697
Msa0589360 MtrunA17_Chr7g0237491 58.025 567 224 4 5 557 14 580 0.0 684
Msa0589360 MtrunA17_Chr7g0237441 56.842 570 232 4 2 557 11 580 0.0 683
Msa0589360 MtrunA17_Chr7g0238351 55.806 577 235 5 1 557 5 581 0.0 671
Msa0589360 MtrunA17_Chr7g0238941 55.810 568 237 6 4 557 11 578 0.0 660
Msa0589360 MtrunA17_Chr4g0031901 55.744 531 225 5 24 545 44 573 0.0 620
Msa0589360 MtrunA17_Chr5g0437681 49.735 565 266 6 7 557 7 567 0.0 589
Msa0589360 MtrunA17_Chr3g0114211 50.353 566 264 8 3 557 11 570 0.0 582
Msa0589360 MtrunA17_Chr5g0431581 50.530 566 265 7 1 557 1 560 0.0 570
Msa0589360 MtrunA17_Chr8g0346581 48.168 546 263 7 24 557 34 571 0.0 538
Msa0589360 MtrunA17_Chr2g0278811 45.979 572 273 5 22 557 36 607 0.0 531
Msa0589360 MtrunA17_Chr4g0008521 47.177 549 275 4 23 557 25 572 7.01e-179 516
Msa0589360 MtrunA17_Chr7g0240971 46.479 568 284 6 8 557 5 570 1.00e-172 501
Msa0589360 MtrunA17_Chr7g0240991 45.073 548 284 8 25 557 26 571 8.10e-172 498
Msa0589360 MtrunA17_Chr5g0436431 59.307 231 90 3 286 515 1 228 4.59e-93 284
Msa0589360 MtrunA17_Chr3g0133861 45.455 330 160 10 226 541 1 324 2.70e-84 265
Msa0589360 MtrunA17_Chr3g0118321 32.042 568 326 17 4 535 2 545 4.01e-73 243
Msa0589360 MtrunA17_Chr3g0118331 30.018 563 343 15 4 535 2 544 9.06e-66 224
Msa0589360 MtrunA17_Chr3g0133871 53.933 178 82 0 25 202 33 210 1.22e-63 207
Msa0589360 MtrunA17_Chr4g0038671 27.928 555 360 13 8 557 13 532 5.81e-56 197
Msa0589360 MtrunA17_Chr3g0143671 28.187 557 357 13 10 557 11 533 3.65e-55 194
Msa0589360 MtrunA17_Chr3g0079321 26.032 557 349 15 27 557 32 551 1.58e-51 186
Msa0589360 MtrunA17_Chr8g0374271 27.841 528 332 14 19 537 26 513 6.54e-51 183
Msa0589360 MtrunA17_Chr1g0157341 27.933 537 349 12 5 537 10 512 1.36e-50 182
Msa0589360 MtrunA17_Chr8g0374261 26.394 538 336 14 1 523 5 497 1.23e-49 179
Msa0589360 MtrunA17_Chr5g0402831 27.473 546 340 18 19 555 26 524 5.77e-49 177
Msa0589360 MtrunA17_Chr4g0024861 27.613 507 319 14 20 517 23 490 7.50e-49 177
Msa0589360 MtrunA17_Chr4g0056511 26.361 588 363 17 1 557 1 549 1.11e-48 178
Msa0589360 MtrunA17_Chr2g0322441 28.797 507 313 14 20 517 26 493 3.14e-47 173
Msa0589360 MtrunA17_Chr4g0075071 29.356 528 322 18 1 517 7 494 4.48e-43 161
Msa0589360 MtrunA17_Chr4g0043261 26.874 547 354 17 1 537 1 511 4.24e-42 159
Msa0589360 MtrunA17_Chr3g0121161 25.185 540 369 16 7 539 47 558 4.66e-41 156
Msa0589360 MtrunA17_Chr3g0121151 25.185 540 369 15 7 539 47 558 1.47e-40 155
Msa0589360 MtrunA17_Chr3g0121171 25.093 538 372 15 7 539 1157 1668 3.18e-40 157
Msa0589360 MtrunA17_Chr3g0121171 25.185 540 369 16 7 539 582 1093 4.48e-40 156
Msa0589360 MtrunA17_Chr3g0121171 25.097 518 359 14 25 539 27 518 7.77e-40 155
Msa0589360 MtrunA17_Chr3g0121171 25.161 465 315 15 80 539 1798 2234 4.85e-33 135
Msa0589360 MtrunA17_Chr7g0234061 25.969 516 333 17 25 522 30 514 7.49e-39 150
Msa0589360 MtrunA17_Chr4g0043251 25.132 569 374 17 7 556 10 545 2.46e-36 142
Msa0589360 MtrunA17_Chr3g0118351 33.436 326 175 11 237 535 6 316 7.23e-35 134
Msa0589360 MtrunA17_Chr8g0382691 41.615 161 90 2 379 535 390 550 2.59e-33 134
Msa0589360 MtrunA17_Chr4g0024641 26.549 452 283 18 76 517 1 413 1.46e-32 133
Msa0589360 MtrunA17_Chr3g0118341 33.529 170 106 5 5 169 4 171 1.60e-25 104
Msa0589360 MtrunA17_Chr5g0436411 63.889 72 26 0 8 79 8 79 3.19e-25 99.4
Msa0589360 MtrunA17_Chr1g0197301 27.077 325 193 10 211 522 1 294 1.08e-22 99.4
Msa0589360 MtrunA17_Chr1g0197311 27.907 172 90 4 27 165 26 196 1.87e-15 76.3
Msa0589360 MtrunA17_Chr3g0118931 48.333 60 31 0 467 526 10 69 3.54e-11 60.5
Msa0589360 MtrunA17_Chr4g0075781 31.884 138 85 3 146 283 21 149 3.85e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0589360 AT2G38080.1 74.727 550 137 2 8 557 11 558 0.0 883
Msa0589360 AT5G01190.1 72.272 559 152 3 1 557 1 558 0.0 820
Msa0589360 AT5G01190.2 70.841 559 146 4 1 557 1 544 0.0 792
Msa0589360 AT5G58910.2 65.832 559 183 4 5 557 11 567 0.0 768
Msa0589360 AT5G58910.3 65.653 559 183 5 5 557 11 566 0.0 762
Msa0589360 AT5G58910.1 66.415 530 163 5 34 557 3 523 0.0 726
Msa0589360 AT5G03260.1 59.459 555 217 4 8 557 6 557 0.0 693
Msa0589360 AT5G60020.1 55.420 572 234 6 7 557 6 577 0.0 658
Msa0589360 AT5G60020.3 57.169 544 214 5 33 557 2 545 0.0 649
Msa0589360 AT2G29130.1 52.880 573 254 7 1 557 1 573 0.0 626
Msa0589360 AT5G05390.1 52.381 567 254 7 2 557 4 565 0.0 606
Msa0589360 AT5G60020.2 54.340 530 221 6 7 515 6 535 0.0 590
Msa0589360 AT2G30210.1 49.030 567 272 6 4 557 8 570 0.0 574
Msa0589360 AT1G18140.1 49.820 556 254 8 24 557 29 581 0.0 571
Msa0589360 AT2G40370.1 49.123 570 257 9 14 557 18 580 0.0 567
Msa0589360 AT5G07130.1 49.741 579 258 10 2 557 1 569 0.0 561
Msa0589360 AT5G09360.1 46.057 558 287 6 7 557 19 569 0.0 531
Msa0589360 AT2G46570.1 45.636 550 281 6 19 557 27 569 0.0 523
Msa0589360 AT5G07130.2 51.417 494 208 9 86 557 1 484 9.47e-170 490
Msa0589360 AT5G07130.3 51.417 494 208 9 86 557 1 484 9.47e-170 490
Msa0589360 AT5G01050.1 44.227 563 293 7 9 557 13 568 2.74e-169 493
Msa0589360 AT5G01040.1 44.207 561 294 8 9 557 13 566 3.63e-169 492
Msa0589360 AT3G09220.1 45.841 541 281 6 10 540 12 550 1.62e-166 485
Msa0589360 AT5G48100.1 45.269 539 279 10 21 547 19 553 5.45e-164 478
Msa0589360 AT2G46570.2 45.287 488 249 6 81 557 4 484 1.84e-157 459
Msa0589360 AT3G09220.2 46.916 454 229 6 97 540 2 453 7.00e-139 411
Msa0589360 AT4G39830.1 33.810 559 334 14 2 535 13 560 2.54e-87 281
Msa0589360 AT5G21105.1 31.699 571 340 13 8 547 22 573 2.52e-75 250
Msa0589360 AT5G21100.1 32.197 528 304 16 39 536 39 542 1.39e-70 237
Msa0589360 AT5G21105.3 31.157 536 322 12 8 515 22 538 2.78e-66 225
Msa0589360 AT4G39830.2 32.773 476 282 13 85 535 3 465 2.36e-62 213
Msa0589360 AT4G39830.3 32.770 473 280 13 88 535 4 463 1.06e-61 211
Msa0589360 AT4G39830.4 31.789 475 275 12 85 535 3 452 7.70e-59 203
Msa0589360 AT1G21850.1 28.872 523 328 14 7 523 12 496 1.54e-52 188
Msa0589360 AT1G21860.1 27.599 529 339 13 1 523 6 496 5.01e-52 186
Msa0589360 AT4G25240.2 28.498 586 346 19 4 557 6 550 7.73e-52 187
Msa0589360 AT4G25240.1 28.498 586 346 19 4 557 6 550 7.73e-52 187
Msa0589360 AT1G76160.1 27.002 537 349 13 7 537 11 510 8.10e-52 186
Msa0589360 AT4G12420.1 25.967 543 341 13 5 522 6 512 1.71e-51 186
Msa0589360 AT4G12420.2 25.967 543 341 13 5 522 6 512 1.71e-51 186
Msa0589360 AT4G22010.1 27.758 562 359 14 5 557 7 530 3.12e-51 184
Msa0589360 AT1G41830.1 27.110 557 363 16 8 557 11 531 6.85e-51 183
Msa0589360 AT1G75790.1 27.110 557 366 15 5 553 6 530 9.98e-51 183
Msa0589360 AT5G66920.1 27.518 556 335 16 20 557 33 538 3.41e-50 181
Msa0589360 AT5G51480.1 26.560 561 343 17 26 557 30 550 2.40e-49 180
Msa0589360 AT4G28090.1 27.490 502 319 14 45 537 49 514 1.08e-48 177
Msa0589360 AT4G37160.1 25.699 572 359 13 9 557 6 534 2.46e-48 176
Msa0589360 AT4G38420.1 27.324 527 327 13 7 517 13 499 4.11e-48 176
Msa0589360 AT5G48450.2 26.582 553 363 16 8 554 13 528 4.40e-47 174
Msa0589360 AT5G48450.1 26.582 553 363 16 8 554 13 528 6.98e-47 174
Msa0589360 AT2G23630.1 27.290 524 334 12 42 557 48 532 8.20e-46 169
Msa0589360 AT2G23630.2 27.290 524 334 12 42 557 53 537 1.11e-45 169
Msa0589360 AT4G38420.3 27.112 509 315 13 7 499 13 481 5.64e-44 163
Msa0589360 AT4G38420.2 26.681 476 304 11 7 471 13 454 3.17e-40 152
Msa0589360 AT5G21105.2 32.081 346 214 6 209 547 51 382 7.74e-39 147
Msa0589360 AT1G55570.1 26.654 529 347 17 1 517 1 500 8.59e-38 147
Msa0589360 AT4G37160.2 25.514 486 297 12 9 471 6 449 1.61e-37 144
Msa0589360 AT3G13390.1 27.888 502 322 18 25 517 29 499 2.61e-37 145
Msa0589360 AT1G55560.1 25.312 561 366 19 6 554 4 523 5.85e-36 142
Msa0589360 AT3G13400.2 24.376 521 357 16 6 517 5 497 2.04e-32 131
Msa0589360 AT3G13400.1 24.376 521 357 16 6 517 5 497 2.04e-32 131

Find 131 sgRNAs with CRISPR-Local

Find 286 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATCACAATTGTTTCAATT+TGG 0.198155 4_2:-12062006 None:intergenic
AAAGCAATTGTCACAGTTAA+TGG 0.198190 4_2:+12060058 Msa0589360:CDS
TAAATGGAGTATGCACATTT+TGG 0.209018 4_2:+12063533 Msa0589360:three_prime_UTR
TCAACAAGGTTAAACTTCTT+TGG 0.219638 4_2:-12062697 None:intergenic
GTTCAAGCATATTTAATAAA+TGG 0.221288 4_2:+12063517 Msa0589360:three_prime_UTR
TTTGGCATGCACATGTTAAT+TGG 0.227901 4_2:+12060718 Msa0589360:CDS
AACATTAACTGGGTATTTCT+TGG 0.229766 4_2:-12062266 None:intergenic
TGTGAGGAATATCAACATTT+TGG 0.230171 4_2:-12063332 None:intergenic
AACCATCCTGTTCATCTTCA+TGG 0.254799 4_2:+12062619 Msa0589360:CDS
TCAGATGCTCATACCATAAA+TGG 0.255242 4_2:+12061176 Msa0589360:CDS
AGATTGTGCATATGTATTAT+TGG 0.263411 4_2:+12063579 Msa0589360:three_prime_UTR
ACATACAATTTCACCATTAC+TGG 0.266011 4_2:+12060678 Msa0589360:CDS
TGTCCCAGGAAGACCATTTA+TGG 0.286955 4_2:-12061189 None:intergenic
GGATAAGGAACACCTTTCTT+TGG 0.297000 4_2:-12060777 None:intergenic
GCCAGATGATGAATTGGTTT+TGG 0.300374 4_2:+12060809 Msa0589360:CDS
TTTCTTTGGTAAAATAACTA+TGG 0.303777 4_2:-12060763 None:intergenic
GTCAAAGAGGAACACTTCTT+TGG 0.310116 4_2:+12060700 Msa0589360:CDS
CCAATTCATCATCTGGCTTT+GGG 0.320192 4_2:-12060803 None:intergenic
ACCAATTCATCATCTGGCTT+TGG 0.321201 4_2:-12060804 None:intergenic
CCCAAAGCCAGATGATGAAT+TGG 0.321450 4_2:+12060803 Msa0589360:CDS
TTATAAATATGAGGTGGATT+TGG 0.329000 4_2:-12062478 None:intergenic
GCAACAGGTGCATCCATAAA+AGG 0.347626 4_2:-12062100 None:intergenic
GGTTTCAATTTCTTTGTTGT+TGG 0.348108 4_2:+12062640 Msa0589360:CDS
CAGCTTGTTATGCAAGATAC+TGG 0.352988 4_2:+12062580 Msa0589360:CDS
TTGGTTCATGCATTGCCATT+TGG 0.358213 4_2:+12063217 Msa0589360:CDS
AGTGAGTGAGTGAGTGAAAA+TGG 0.365147 4_2:+12059145 Msa0589360:exon
GGTGTTTGGTTTAATACAAA+AGG 0.369465 4_2:+12063629 Msa0589360:three_prime_UTR
TCTTGTATTATATAGGAGTT+TGG 0.374663 4_2:+12063198 Msa0589360:intron
ATTTATAATTTCACTGGTGC+TGG 0.376600 4_2:+12062493 Msa0589360:CDS
ACAGCTACATTACATTATTC+AGG 0.378100 4_2:+12062142 Msa0589360:CDS
GGAGCTATCACTGATTGTTT+AGG 0.383191 4_2:-12063296 None:intergenic
TTGTTGCATATTTCCAGCTT+TGG 0.394093 4_2:+12059196 Msa0589360:CDS
CACAGTTAATGGGAAGTTCC+CGG 0.400965 4_2:+12060069 Msa0589360:CDS
TAACTATGTCCTGGTTGAAT+TGG 0.405627 4_2:-12060654 None:intergenic
ATCAATGAAGCTCTAAAATC+AGG 0.405686 4_2:+12061137 Msa0589360:CDS
GGAGGTGTAGTTAGAAATGT+AGG 0.406581 4_2:-12062184 None:intergenic
CCAATAGTGTTCCTTTCAAC+AGG 0.406648 4_2:-12062721 None:intergenic
CATACCATAAATGGTCTTCC+TGG 0.407581 4_2:+12061185 Msa0589360:CDS
CTTCTACTACTGTGAGTTTG+TGG 0.408210 4_2:-12061958 None:intergenic
AATACCTTGTGTAGAACAAT+TGG 0.408385 4_2:-12061216 None:intergenic
TTTGTTGTTGGTAAAGGAGT+TGG 0.409860 4_2:+12062652 Msa0589360:CDS
TTGAAACCATGAAGATGAAC+AGG 0.413774 4_2:-12062625 None:intergenic
AATTTCTTTGTTGTTGGTAA+AGG 0.416507 4_2:+12062646 Msa0589360:CDS
CAGCATTGATGATTCTTAGT+AGG 0.420767 4_2:-12061901 None:intergenic
GAACAATTGGCCACTGTCCC+AGG 0.420964 4_2:-12061203 None:intergenic
ACCAAAACCAATTCATCATC+TGG 0.422160 4_2:-12060810 None:intergenic
CGGGACCCACTCTTTATGCC+AGG 0.431604 4_2:+12060089 Msa0589360:CDS
GTTAAATTGTCTACAGCAAC+AGG 0.433150 4_2:-12062115 None:intergenic
ATATTGGCAGGCATGGTATT+AGG 0.442333 4_2:+12060583 Msa0589360:intron
AAGCAATTGTCACAGTTAAT+GGG 0.447103 4_2:+12060059 Msa0589360:CDS
TGGTGTACCAGCTGGTGGTT+GGG 0.453591 4_2:+12062741 Msa0589360:CDS
GTGTTTGGTTTAATACAAAA+GGG 0.459483 4_2:+12063630 Msa0589360:three_prime_UTR
CCTCATATTTATAATTTCAC+TGG 0.464423 4_2:+12062487 Msa0589360:CDS
AGTCAAAAGTGTACACTTGT+TGG 0.467075 4_2:-12063604 None:intergenic
ACTTTGCACTCGACCAAAGC+TGG 0.472524 4_2:-12059209 None:intergenic
AGCTTATGATAGTGACCAAA+GGG 0.473932 4_2:-12063757 None:intergenic
TAAATATGAGGTGGATTTGG+TGG 0.477812 4_2:-12062475 None:intergenic
TCTGGCTTTGGGAAAGGATA+AGG 0.480287 4_2:-12060792 None:intergenic
TTGAGGACAGGTTGGGCTGA+TGG 0.489336 4_2:+12060609 Msa0589360:CDS
TTGGTGTACCAGCTGGTGGT+TGG 0.489399 4_2:+12062740 Msa0589360:CDS
TAGGCAATTGAGGACAGGTT+GGG 0.490554 4_2:+12060602 Msa0589360:CDS
TGTTCCTCTTTGACCAGTAA+TGG 0.494174 4_2:-12060691 None:intergenic
AGATGAACAGGATGGTTCTC+AGG 0.496558 4_2:-12062613 None:intergenic
CTTTGGTCGAGTGCAAAGTT+AGG 0.497718 4_2:+12059213 Msa0589360:CDS
TGGCATAAAGAGTGGGTCCC+GGG 0.497927 4_2:-12060087 None:intergenic
CCTTCATGTAAAGCTGCAAA+TGG 0.499621 4_2:+12062346 Msa0589360:CDS
TCATCATCTGGCTTTGGGAA+AGG 0.504275 4_2:-12060798 None:intergenic
ATTGATGATTCTTAGTAGGT+AGG 0.505350 4_2:-12061897 None:intergenic
GTTCATGCATTGCCATTTGG+AGG 0.507146 4_2:+12063220 Msa0589360:CDS
TCATTGGTGAATGCAACTTA+AGG 0.508951 4_2:+12059077 None:intergenic
GTATGAGCATCTGAAACATT+TGG 0.509634 4_2:-12061167 None:intergenic
CCATTTGCAGCTTTACATGA+AGG 0.512374 4_2:-12062346 None:intergenic
ATGTTCATACAATTCGTAGT+AGG 0.515285 4_2:+12063359 Msa0589360:three_prime_UTR
ACAATTGTTTCAATTTGGAA+TGG 0.524020 4_2:-12062001 None:intergenic
TACAACATGGGGTCTAAAGA+TGG 0.524585 4_2:+12063247 Msa0589360:CDS
CCTTCCAAATTCACAAAAGT+AGG 0.529000 4_2:+12063382 Msa0589360:three_prime_UTR
ATTTGGAGGTGCATACAACA+TGG 0.531146 4_2:+12063234 Msa0589360:CDS
ATGTTGTATGCACCTCCAAA+TGG 0.535908 4_2:-12063232 None:intergenic
ACAGTTAATGGGAAGTTCCC+GGG 0.536821 4_2:+12060070 Msa0589360:CDS
AGTACATTTGTTGTTTGACC+AGG 0.537617 4_2:-12062031 None:intergenic
CCAGTGAAATTATAAATATG+AGG 0.540266 4_2:-12062487 None:intergenic
TTCACCATTACTGGTCAAAG+AGG 0.543070 4_2:+12060687 Msa0589360:CDS
CTGGCATAAAGAGTGGGTCC+CGG 0.543634 4_2:-12060088 None:intergenic
ACCACAATATTACCATCCAT+TGG 0.545580 4_2:+12060152 Msa0589360:CDS
TTCACAAAAGTAGGTGTGGA+GGG 0.546959 4_2:+12063391 Msa0589360:three_prime_UTR
GGCATGGTATTAGGCAATTG+AGG 0.551664 4_2:+12060592 Msa0589360:intron
AACACTATTGGTGTACCAGC+TGG 0.553147 4_2:+12062733 Msa0589360:CDS
GGTATTAGGCAATTGAGGAC+AGG 0.554052 4_2:+12060597 Msa0589360:CDS
TTGATTAGTTTAGCTAAATG+TGG 0.556260 4_2:+12063673 Msa0589360:three_prime_UTR
CCTGTTGAAAGGAACACTAT+TGG 0.559502 4_2:+12062721 Msa0589360:CDS
TGGAGGGATACAGAGGAGCC+AGG 0.560611 4_2:+12063407 Msa0589360:three_prime_UTR
CAAATTCACAAAAGTAGGTG+TGG 0.563160 4_2:+12063387 Msa0589360:three_prime_UTR
AGATTCAGAGCTGATAATCC+AGG 0.563607 4_2:+12062772 Msa0589360:CDS
AAATGGTCTTCCTGGGACAG+TGG 0.566886 4_2:+12061193 Msa0589360:CDS
GAGGTGTAGTTAGAAATGTA+GGG 0.568648 4_2:-12062183 None:intergenic
TCCTCCCTGGCATAAAGAGT+GGG 0.570395 4_2:-12060094 None:intergenic
ATACCATAAATGGTCTTCCT+GGG 0.571091 4_2:+12061186 Msa0589360:CDS
ACCTTGTTGATCCTGTTGAA+AGG 0.571542 4_2:+12062710 Msa0589360:CDS
TTAGGCAATTGAGGACAGGT+TGG 0.574915 4_2:+12060601 Msa0589360:CDS
TGGCTAAGATCAACTGTCCA+TGG 0.575480 4_2:+12060738 Msa0589360:CDS
TGAAGCTCTAAAATCAGGAA+TGG 0.584529 4_2:+12061142 Msa0589360:CDS
GGGACCCACTCTTTATGCCA+GGG 0.585095 4_2:+12060090 Msa0589360:CDS
ATTGCAACCCAACCACCAGC+TGG 0.593002 4_2:-12062748 None:intergenic
TAGCTTATGATAGTGACCAA+AGG 0.595791 4_2:-12063758 None:intergenic
GAAACAATTGTGATAGCACC+TGG 0.597555 4_2:+12062013 Msa0589360:CDS
ATCCTCCCTGGCATAAAGAG+TGG 0.601028 4_2:-12060095 None:intergenic
TATGTTGTCTAACCAATGGA+TGG 0.606132 4_2:-12060164 None:intergenic
GCTTGTAGTAAAGCTGTAGT+TGG 0.610290 4_2:-12062412 None:intergenic
ATTCACAAAAGTAGGTGTGG+AGG 0.612600 4_2:+12063390 Msa0589360:three_prime_UTR
GTAGGTGTGGAGGGATACAG+AGG 0.617116 4_2:+12063400 Msa0589360:three_prime_UTR
TTTGGAGGTGCATACAACAT+GGG 0.618425 4_2:+12063235 Msa0589360:CDS
TGAATAATGTAATGTAGCTG+TGG 0.621640 4_2:-12062140 None:intergenic
TATCACTGTATCATCCTCCC+TGG 0.622357 4_2:-12060107 None:intergenic
GTGAAATTATAAATATGAGG+TGG 0.624519 4_2:-12062484 None:intergenic
AGGTATTCACAATTATTGAG+AGG 0.624881 4_2:+12063723 Msa0589360:three_prime_UTR
TCCTTTCAACAGGATCAACA+AGG 0.625266 4_2:-12062711 None:intergenic
TTGTATGTATAACTATGTCC+TGG 0.632359 4_2:-12060663 None:intergenic
GCAGCTTTACATGAAGGACA+AGG 0.639189 4_2:-12062340 None:intergenic
ACCAATGGATGGTAATATTG+TGG 0.640439 4_2:-12060153 None:intergenic
GGACACTGTGTGATATATGC+AGG 0.645297 4_2:-12060633 None:intergenic
GTAAAATAACTATGGCACCA+TGG 0.647215 4_2:-12060755 None:intergenic
TACAAACTACCTGTGGAGAG+TGG 0.647512 4_2:+12061869 Msa0589360:CDS
TTGTATGAACATTTGATGTG+AGG 0.663372 4_2:-12063348 None:intergenic
TTGGAGGTGCATACAACATG+GGG 0.676626 4_2:+12063236 Msa0589360:CDS
AACCATGAAGATGAACAGGA+TGG 0.677352 4_2:-12062621 None:intergenic
AGGTGTAGTTAGAAATGTAG+GGG 0.680436 4_2:-12062182 None:intergenic
ACACAGTGTCCAATTCAACC+AGG 0.693720 4_2:+12060645 Msa0589360:CDS
GTGGCCAATTGTTCTACACA+AGG 0.704099 4_2:+12061212 Msa0589360:CDS
ACTATTGGTGTACCAGCTGG+TGG 0.711640 4_2:+12062736 Msa0589360:CDS
ACCCACTCTTTATGCCAGGG+AGG 0.713504 4_2:+12060093 Msa0589360:CDS
AGATGTTTACAAACTACCTG+TGG 0.715278 4_2:+12061862 Msa0589360:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCTCATAATTAATATT+AGG + chr4_2:12059418-12059437 Msa0589360:intron 10.0%
!! ATTTCTCATAATTAATATTA+GGG + chr4_2:12059419-12059438 Msa0589360:intron 10.0%
!!! TTATTTCTGAAATATTATAT+GGG - chr4_2:12059664-12059683 None:intergenic 10.0%
!!! ATTATTTCTGAAATATTATA+TGG - chr4_2:12059665-12059684 None:intergenic 10.0%
!! ATAGCTTATAAATATAATAT+GGG - chr4_2:12059746-12059765 None:intergenic 10.0%
!!! GTTTTTTTTTTTTTTTTAAG+GGG - chr4_2:12060409-12060428 None:intergenic 10.0%
!! TAAAAATAAAGTGAATTATT+TGG - chr4_2:12060441-12060460 None:intergenic 10.0%
!! AAAATTAAACTAATTAACAA+AGG - chr4_2:12060877-12060896 None:intergenic 10.0%
!!! CTTAATAAATAGAAAATTTT+AGG + chr4_2:12061356-12061375 Msa0589360:intron 10.0%
!!! TTTTTTTTTTCTTTCTTATT+TGG + chr4_2:12061829-12061848 Msa0589360:intron 10.0%
!!! TCTTTAAGATTTCTAAATTA+GGG + chr4_2:12059469-12059488 Msa0589360:intron 15.0%
!! GATAGCTTATAAATATAATA+TGG - chr4_2:12059747-12059766 None:intergenic 15.0%
!!! TAATTTTTGACAATCAATTT+TGG + chr4_2:12059978-12059997 Msa0589360:intron 15.0%
!! ATTGAGTATCAATAATAAAA+CGG - chr4_2:12060310-12060329 None:intergenic 15.0%
!!! TTATTGATACTCAATATTTT+AGG + chr4_2:12060315-12060334 Msa0589360:intron 15.0%
!!! TATTGATACTCAATATTTTA+GGG + chr4_2:12060316-12060335 Msa0589360:intron 15.0%
!!! ATTATCTATTTTTAGAGTAA+AGG - chr4_2:12060485-12060504 None:intergenic 15.0%
!!! TAACAAAGGATTTTTTTTTT+TGG - chr4_2:12060863-12060882 None:intergenic 15.0%
!! ATTCTAATAAATGTTTATGA+TGG + chr4_2:12061062-12061081 Msa0589360:intron 15.0%
!! ATTCTCTATAAATATTATCA+AGG + chr4_2:12061260-12061279 Msa0589360:intron 15.0%
!! TTCTCTATAAATATTATCAA+GGG + chr4_2:12061261-12061280 Msa0589360:intron 15.0%
!!! TCATGAATATGATTATTTTT+GGG + chr4_2:12061414-12061433 Msa0589360:intron 15.0%
!!! GTATTTTATCCTTATAAAAT+TGG - chr4_2:12061511-12061530 None:intergenic 15.0%
!!! AATTTTTTAAACTCACTTAA+AGG + chr4_2:12062235-12062254 Msa0589360:CDS 15.0%
!!! AGATTTAACAAAATTTTAAC+CGG + chr4_2:12062918-12062937 Msa0589360:intron 15.0%
!! AAATTGATTAAACAAAACAA+CGG - chr4_2:12062949-12062968 None:intergenic 15.0%
!!! ATTTCTCATTGAAATTTTTA+AGG + chr4_2:12063117-12063136 Msa0589360:intron 15.0%
!! AATTATTAAACCCTATTAAA+AGG - chr4_2:12063169-12063188 None:intergenic 15.0%
!! TACAAATTTCATGTAAGTAA+TGG + chr4_2:12059239-12059258 Msa0589360:intron 20.0%
!!! CTCTTTAAGATTTCTAAATT+AGG + chr4_2:12059468-12059487 Msa0589360:intron 20.0%
!! ATAAGCTATCATCATTATTA+CGG + chr4_2:12059757-12059776 Msa0589360:intron 20.0%
!!! TTTTCTCATTGTGTTATATA+TGG - chr4_2:12059823-12059842 None:intergenic 20.0%
!!! TGCATAGAATTAACTAATTT+TGG + chr4_2:12059949-12059968 Msa0589360:intron 20.0%
!!! ACATAATCTAGTTGTATTTT+TGG - chr4_2:12060032-12060051 None:intergenic 20.0%
!!! TTTTTTTTTTTTTAAGGGGT+TGG - chr4_2:12060405-12060424 None:intergenic 20.0%
!! TTACTCTATTATTACTAGTT+TGG + chr4_2:12060458-12060477 Msa0589360:intron 20.0%
!! TTGAGTCATTATTATCATAT+TGG + chr4_2:12060567-12060586 Msa0589360:intron 20.0%
!! TTTCTTTGGTAAAATAACTA+TGG - chr4_2:12060766-12060785 None:intergenic 20.0%
!!! ATAGTTATTTTACCAAAGAA+AGG + chr4_2:12060765-12060784 Msa0589360:CDS 20.0%
!! ATGTTTCCATTAACATTAAT+TGG + chr4_2:12061038-12061057 Msa0589360:intron 20.0%
!! AGAATTCCAATTAATGTTAA+TGG - chr4_2:12061047-12061066 None:intergenic 20.0%
!!! ACCTTTTTCAATTTTGTATA+GGG - chr4_2:12061304-12061323 None:intergenic 20.0%
!!! TACCTTTTTCAATTTTGTAT+AGG - chr4_2:12061305-12061324 None:intergenic 20.0%
!! ATACAAAATTGAAAAAGGTA+TGG + chr4_2:12061305-12061324 Msa0589360:intron 20.0%
!!! GTCATGAATATGATTATTTT+TGG + chr4_2:12061413-12061432 Msa0589360:intron 20.0%
!!! CATGAATATGATTATTTTTG+GGG + chr4_2:12061415-12061434 Msa0589360:intron 20.0%
!!! ATGAATATGATTATTTTTGG+GGG + chr4_2:12061416-12061435 Msa0589360:intron 20.0%
!!! CAAAATTCTCCAATTTTATA+AGG + chr4_2:12061499-12061518 Msa0589360:intron 20.0%
!!! GGTTATATTTTATTGTTGTT+AGG - chr4_2:12061801-12061820 None:intergenic 20.0%
!! AAGAAAAAAAAAACATGCTT+AGG - chr4_2:12061822-12061841 None:intergenic 20.0%
!!! CTAAATATTTTCCAGATTTT+TGG - chr4_2:12062069-12062088 None:intergenic 20.0%
!!! TTTTTAAAGGAACATTAACT+GGG - chr4_2:12062279-12062298 None:intergenic 20.0%
!!! ATTTTTAAAGGAACATTAAC+TGG - chr4_2:12062280-12062299 None:intergenic 20.0%
!!! AGTGAATGATCAATTTTTAA+AGG - chr4_2:12062292-12062311 None:intergenic 20.0%
!!! TTTTTTTTACTGTTGGATTA+GGG + chr4_2:12062311-12062330 Msa0589360:CDS 20.0%
!!! AGAAAATAAGTATTTTTACC+TGG - chr4_2:12062793-12062812 None:intergenic 20.0%
!!! GATTTAACAAAATTTTAACC+GGG + chr4_2:12062919-12062938 Msa0589360:intron 20.0%
!!! TTGTTTTAAAATTGTAGCAA+AGG - chr4_2:12063069-12063088 None:intergenic 20.0%
!! TTCAATGTCTTGTATTATAT+AGG + chr4_2:12063191-12063210 Msa0589360:intron 20.0%
!! CTCATTCAAAAAACAATTTA+TGG - chr4_2:12063482-12063501 None:intergenic 20.0%
!! GTTCAAGCATATTTAATAAA+TGG + chr4_2:12063517-12063536 Msa0589360:three_prime_UTR 20.0%
! GTATTATTAATAATGAGTGC+AGG + chr4_2:12060000-12060019 Msa0589360:intron 25.0%
!!! TGATTAGATCTTTTGCTATA+TGG - chr4_2:12060518-12060537 None:intergenic 25.0%
! TCCCTATACAAAATTGAAAA+AGG + chr4_2:12061300-12061319 Msa0589360:intron 25.0%
! AGATAAATAAATACACGCAA+AGG - chr4_2:12061752-12061771 None:intergenic 25.0%
! ACAATTGTTTCAATTTGGAA+TGG - chr4_2:12062004-12062023 None:intergenic 25.0%
! CTATCACAATTGTTTCAATT+TGG - chr4_2:12062009-12062028 None:intergenic 25.0%
! TTAAAAGCAAACCAAAAATC+TGG + chr4_2:12062055-12062074 Msa0589360:CDS 25.0%
!!! CATTCACTTTTTTTTACTGT+TGG + chr4_2:12062304-12062323 Msa0589360:CDS 25.0%
!!! CTTTTTTTTACTGTTGGATT+AGG + chr4_2:12062310-12062329 Msa0589360:CDS 25.0%
!!! TTTTTTTACTGTTGGATTAG+GGG + chr4_2:12062312-12062331 Msa0589360:CDS 25.0%
!! TTATAAATATGAGGTGGATT+TGG - chr4_2:12062481-12062500 None:intergenic 25.0%
! GTGAAATTATAAATATGAGG+TGG - chr4_2:12062487-12062506 None:intergenic 25.0%
! CCAGTGAAATTATAAATATG+AGG - chr4_2:12062490-12062509 None:intergenic 25.0%
! CCTCATATTTATAATTTCAC+TGG + chr4_2:12062487-12062506 Msa0589360:CDS 25.0%
!!! AATTTCTTTGTTGTTGGTAA+AGG + chr4_2:12062646-12062665 Msa0589360:CDS 25.0%
! TCTTGTATTATATAGGAGTT+TGG + chr4_2:12063198-12063217 Msa0589360:intron 25.0%
! CAAAAATAAAAGAGAGTGAA+GGG + chr4_2:12063430-12063449 Msa0589360:three_prime_UTR 25.0%
! AGATTGTGCATATGTATTAT+TGG + chr4_2:12063579-12063598 Msa0589360:three_prime_UTR 25.0%
!! GTGTTTGGTTTAATACAAAA+GGG + chr4_2:12063630-12063649 Msa0589360:three_prime_UTR 25.0%
!!! TTGATTAGTTTAGCTAAATG+TGG + chr4_2:12063673-12063692 Msa0589360:three_prime_UTR 25.0%
!!! CAATTTTTTTTCGTTTTGAG+AGG + chr4_2:12063700-12063719 Msa0589360:three_prime_UTR 25.0%
! GAATTTTCTTTTGCAACTTC+AGG - chr4_2:12059104-12059123 None:intergenic 30.0%
CACTAGTTAAGGACTTTAAA+TGG - chr4_2:12059617-12059636 None:intergenic 30.0%
ATTATTAGAATGTGCATGTG+TGG - chr4_2:12059923-12059942 None:intergenic 30.0%
TTATTAATAATGAGTGCAGG+TGG + chr4_2:12060003-12060022 Msa0589360:intron 30.0%
AAAGCAATTGTCACAGTTAA+TGG + chr4_2:12060058-12060077 Msa0589360:CDS 30.0%
AAGCAATTGTCACAGTTAAT+GGG + chr4_2:12060059-12060078 Msa0589360:CDS 30.0%
AGAATATGTTGTCTAACCAA+TGG - chr4_2:12060171-12060190 None:intergenic 30.0%
!! ATCTTTTGCTATATGGTCAA+AGG - chr4_2:12060511-12060530 None:intergenic 30.0%
GTCATTATTATCATATTGGC+AGG + chr4_2:12060571-12060590 Msa0589360:intron 30.0%
TTGTATGTATAACTATGTCC+TGG - chr4_2:12060666-12060685 None:intergenic 30.0%
ACATACAATTTCACCATTAC+TGG + chr4_2:12060678-12060697 Msa0589360:CDS 30.0%
!!! GATGAATTGGTTTTGGTATT+AGG + chr4_2:12060816-12060835 Msa0589360:CDS 30.0%
GGTGATGATTGAAAAATTGT+AGG + chr4_2:12061083-12061102 Msa0589360:intron 30.0%
ATTGAAAAATTGTAGGAGAG+TGG + chr4_2:12061090-12061109 Msa0589360:intron 30.0%
!! ATCAATGAAGCTCTAAAATC+AGG + chr4_2:12061137-12061156 Msa0589360:CDS 30.0%
AATACCTTGTGTAGAACAAT+TGG - chr4_2:12061219-12061238 None:intergenic 30.0%
!!! AATAGTGTGCTTTTAGTTTC+TGG - chr4_2:12061465-12061484 None:intergenic 30.0%
! ACTAAAAGCACACTATTGTT+AGG + chr4_2:12061468-12061487 Msa0589360:intron 30.0%
GACTCATATTCACCAATAAA+TGG + chr4_2:12061558-12061577 Msa0589360:intron 30.0%
TGTTGAATATGCAATGCTTA+GGG - chr4_2:12061664-12061683 None:intergenic 30.0%
ATGTTGAATATGCAATGCTT+AGG - chr4_2:12061665-12061684 None:intergenic 30.0%
! ATTGATGATTCTTAGTAGGT+AGG - chr4_2:12061900-12061919 None:intergenic 30.0%
! GAACTTTTCTTCAAAATAGC+TGG + chr4_2:12061935-12061954 Msa0589360:CDS 30.0%
TGAATAATGTAATGTAGCTG+TGG - chr4_2:12062143-12062162 None:intergenic 30.0%
ACAGCTACATTACATTATTC+AGG + chr4_2:12062142-12062161 Msa0589360:CDS 30.0%
!! ATTTGTGTAGCATTTTTAGG+TGG - chr4_2:12062208-12062227 None:intergenic 30.0%
! GCAATTTGTGTAGCATTTTT+AGG - chr4_2:12062211-12062230 None:intergenic 30.0%
AACATTAACTGGGTATTTCT+TGG - chr4_2:12062269-12062288 None:intergenic 30.0%
ATTTATAATTTCACTGGTGC+TGG + chr4_2:12062493-12062512 Msa0589360:CDS 30.0%
!! CTTGTAGTGTTCAAGTTTTT+AGG - chr4_2:12062520-12062539 None:intergenic 30.0%
AAAAACTTGAACACTACAAG+TGG + chr4_2:12062520-12062539 Msa0589360:CDS 30.0%
! GGTTTCAATTTCTTTGTTGT+TGG + chr4_2:12062640-12062659 Msa0589360:CDS 30.0%
TCAACAAGGTTAAACTTCTT+TGG - chr4_2:12062700-12062719 None:intergenic 30.0%
ATAATCGAGATCAAATCAAC+AGG - chr4_2:12062879-12062898 None:intergenic 30.0%
CATTGTTTAATTGGTGCAAT+CGG - chr4_2:12063023-12063042 None:intergenic 30.0%
! CTTGTACTTGCCTTTTAATA+GGG + chr4_2:12063156-12063175 Msa0589360:intron 30.0%
!! CAACATTTTGGAAGATCTTT+TGG - chr4_2:12063323-12063342 None:intergenic 30.0%
! TGTGAGGAATATCAACATTT+TGG - chr4_2:12063335-12063354 None:intergenic 30.0%
TTGTATGAACATTTGATGTG+AGG - chr4_2:12063351-12063370 None:intergenic 30.0%
ATGTTCATACAATTCGTAGT+AGG + chr4_2:12063359-12063378 Msa0589360:three_prime_UTR 30.0%
GCAAAAATAAAAGAGAGTGA+AGG + chr4_2:12063429-12063448 Msa0589360:three_prime_UTR 30.0%
!!! TGTTTTTTGAATGAGCAGAA+AGG + chr4_2:12063487-12063506 Msa0589360:three_prime_UTR 30.0%
! TAAATGGAGTATGCACATTT+TGG + chr4_2:12063533-12063552 Msa0589360:three_prime_UTR 30.0%
! GGTGTTTGGTTTAATACAAA+AGG + chr4_2:12063629-12063648 Msa0589360:three_prime_UTR 30.0%
!!! TTTTTTTTCGTTTTGAGAGG+AGG + chr4_2:12063703-12063722 Msa0589360:three_prime_UTR 30.0%
AGGTATTCACAATTATTGAG+AGG + chr4_2:12063723-12063742 Msa0589360:three_prime_UTR 30.0%
TTGTTGCATATTTCCAGCTT+TGG + chr4_2:12059196-12059215 Msa0589360:CDS 35.0%
!! CTTTAGGTGCTGATTATAGA+GGG - chr4_2:12059874-12059893 None:intergenic 35.0%
!! ACTTTAGGTGCTGATTATAG+AGG - chr4_2:12059875-12059894 None:intergenic 35.0%
CTATAATCAGCACCTAAAGT+TGG + chr4_2:12059875-12059894 Msa0589360:intron 35.0%
ACCACAATATTACCATCCAT+TGG + chr4_2:12060152-12060171 Msa0589360:CDS 35.0%
ACCAATGGATGGTAATATTG+TGG - chr4_2:12060156-12060175 None:intergenic 35.0%
TATGTTGTCTAACCAATGGA+TGG - chr4_2:12060167-12060186 None:intergenic 35.0%
TATTATCATATTGGCAGGCA+TGG + chr4_2:12060576-12060595 Msa0589360:intron 35.0%
TAACTATGTCCTGGTTGAAT+TGG - chr4_2:12060657-12060676 None:intergenic 35.0%
TTTGGCATGCACATGTTAAT+TGG + chr4_2:12060718-12060737 Msa0589360:CDS 35.0%
GTAAAATAACTATGGCACCA+TGG - chr4_2:12060758-12060777 None:intergenic 35.0%
ACCAAAACCAATTCATCATC+TGG - chr4_2:12060813-12060832 None:intergenic 35.0%
!! TGAAGCTCTAAAATCAGGAA+TGG + chr4_2:12061142-12061161 Msa0589360:CDS 35.0%
! GTATGAGCATCTGAAACATT+TGG - chr4_2:12061170-12061189 None:intergenic 35.0%
TCAGATGCTCATACCATAAA+TGG + chr4_2:12061176-12061195 Msa0589360:CDS 35.0%
ATACCATAAATGGTCTTCCT+GGG + chr4_2:12061186-12061205 Msa0589360:CDS 35.0%
TTGAGTGACTGTCCATTTAT+TGG - chr4_2:12061573-12061592 None:intergenic 35.0%
ATCTTATGAACATGAAGAGC+AGG - chr4_2:12061615-12061634 None:intergenic 35.0%
AGATGTTTACAAACTACCTG+TGG + chr4_2:12061862-12061881 Msa0589360:intron 35.0%
!! CAGCATTGATGATTCTTAGT+AGG - chr4_2:12061904-12061923 None:intergenic 35.0%
!! AGTACATTTGTTGTTTGACC+AGG - chr4_2:12062034-12062053 None:intergenic 35.0%
GTTAAATTGTCTACAGCAAC+AGG - chr4_2:12062118-12062137 None:intergenic 35.0%
AGGTGTAGTTAGAAATGTAG+GGG - chr4_2:12062185-12062204 None:intergenic 35.0%
GAGGTGTAGTTAGAAATGTA+GGG - chr4_2:12062186-12062205 None:intergenic 35.0%
! TAAATATGAGGTGGATTTGG+TGG - chr4_2:12062478-12062497 None:intergenic 35.0%
TTGAAACCATGAAGATGAAC+AGG - chr4_2:12062628-12062647 None:intergenic 35.0%
!! TTTGTTGTTGGTAAAGGAGT+TGG + chr4_2:12062652-12062671 Msa0589360:CDS 35.0%
AAAATGCCGATCAATTCAAC+AGG + chr4_2:12062834-12062853 Msa0589360:intron 35.0%
CCTCAGGATCATTGTTTAAT+TGG - chr4_2:12063032-12063051 None:intergenic 35.0%
CCAATTAAACAATGATCCTG+AGG + chr4_2:12063029-12063048 Msa0589360:intron 35.0%
CCTATTAAAAGGCAAGTACA+AGG - chr4_2:12063158-12063177 None:intergenic 35.0%
! CCTTGTACTTGCCTTTTAAT+AGG + chr4_2:12063155-12063174 Msa0589360:intron 35.0%
!! TCTAAAGATGGCATTTTTGG+TGG + chr4_2:12063259-12063278 Msa0589360:CDS 35.0%
!! CATTTTGGAAGATCTTTTGG+TGG - chr4_2:12063320-12063339 None:intergenic 35.0%
!! CCTACTTTTGTGAATTTGGA+AGG - chr4_2:12063385-12063404 None:intergenic 35.0%
CCTTCCAAATTCACAAAAGT+AGG + chr4_2:12063382-12063401 Msa0589360:three_prime_UTR 35.0%
!! CACACCTACTTTTGTGAATT+TGG - chr4_2:12063389-12063408 None:intergenic 35.0%
CAAATTCACAAAAGTAGGTG+TGG + chr4_2:12063387-12063406 Msa0589360:three_prime_UTR 35.0%
!!! CACTCTCTTTTATTTTTGCC+TGG - chr4_2:12063428-12063447 None:intergenic 35.0%
AAAGAGAGTGAAGGGAAAAA+AGG + chr4_2:12063438-12063457 Msa0589360:three_prime_UTR 35.0%
! AGTCAAAAGTGTACACTTGT+TGG - chr4_2:12063607-12063626 None:intergenic 35.0%
!!! CAAGTGTACACTTTTGACTT+TGG + chr4_2:12063608-12063627 Msa0589360:three_prime_UTR 35.0%
!!! ACACTTTTGACTTTGGTGTT+TGG + chr4_2:12063615-12063634 Msa0589360:three_prime_UTR 35.0%
GAGGAAAAATATACACCCTT+TGG + chr4_2:12063742-12063761 Msa0589360:three_prime_UTR 35.0%
AGTGAGTGAGTGAGTGAAAA+TGG + chr4_2:12059145-12059164 Msa0589360:exon 40.0%
!! AGAAATCTTAAAGAGTGCCC+CGG - chr4_2:12059463-12059482 None:intergenic 40.0%
!! AGAAATCTTAAAGAGTGCCC+CGG - chr4_2:12059463-12059482 None:intergenic 40.0%
AAAGTCCTTAACTAGTGTCC+CGG + chr4_2:12059620-12059639 Msa0589360:intron 40.0%
AAATGTTAACTAGTGCCTCC+GGG - chr4_2:12059641-12059660 None:intergenic 40.0%
GAAATGTTAACTAGTGCCTC+CGG - chr4_2:12059642-12059661 None:intergenic 40.0%
AGGAGTGATGAACCAACTTT+AGG - chr4_2:12059890-12059909 None:intergenic 40.0%
TTAGAATGTGCATGTGTGGT+TGG - chr4_2:12059919-12059938 None:intergenic 40.0%
TTAATAATGAGTGCAGGTGG+TGG + chr4_2:12060006-12060025 Msa0589360:intron 40.0%
ATATTGGCAGGCATGGTATT+AGG + chr4_2:12060583-12060602 Msa0589360:intron 40.0%
TTCACCATTACTGGTCAAAG+AGG + chr4_2:12060687-12060706 Msa0589360:CDS 40.0%
TGTTCCTCTTTGACCAGTAA+TGG - chr4_2:12060694-12060713 None:intergenic 40.0%
GTCAAAGAGGAACACTTCTT+TGG + chr4_2:12060700-12060719 Msa0589360:CDS 40.0%
GGATAAGGAACACCTTTCTT+TGG - chr4_2:12060780-12060799 None:intergenic 40.0%
!! CCAATTCATCATCTGGCTTT+GGG - chr4_2:12060806-12060825 None:intergenic 40.0%
! ACCAATTCATCATCTGGCTT+TGG - chr4_2:12060807-12060826 None:intergenic 40.0%
!! GCCAGATGATGAATTGGTTT+TGG + chr4_2:12060809-12060828 Msa0589360:CDS 40.0%
GAAAAATTGTAGGAGAGTGG+TGG + chr4_2:12061093-12061112 Msa0589360:intron 40.0%
CATACCATAAATGGTCTTCC+TGG + chr4_2:12061185-12061204 Msa0589360:CDS 40.0%
CTTCTACTACTGTGAGTTTG+TGG - chr4_2:12061961-12061980 None:intergenic 40.0%
GAAACAATTGTGATAGCACC+TGG + chr4_2:12062013-12062032 Msa0589360:CDS 40.0%
! AGTAGCTGCTTCACCTTTTA+TGG + chr4_2:12062087-12062106 Msa0589360:CDS 40.0%
! GGAGGTGTAGTTAGAAATGT+AGG - chr4_2:12062187-12062206 None:intergenic 40.0%
!!! TGTGTAGCATTTTTAGGTGG+AGG - chr4_2:12062205-12062224 None:intergenic 40.0%
CCATTTGCAGCTTTACATGA+AGG - chr4_2:12062349-12062368 None:intergenic 40.0%
CCTTCATGTAAAGCTGCAAA+TGG + chr4_2:12062346-12062365 Msa0589360:CDS 40.0%
GCTTGTAGTAAAGCTGTAGT+TGG - chr4_2:12062415-12062434 None:intergenic 40.0%
!! CAGCTTGTTATGCAAGATAC+TGG + chr4_2:12062580-12062599 Msa0589360:CDS 40.0%
AACCATCCTGTTCATCTTCA+TGG + chr4_2:12062619-12062638 Msa0589360:CDS 40.0%
AACCATGAAGATGAACAGGA+TGG - chr4_2:12062624-12062643 None:intergenic 40.0%
ACCTTGTTGATCCTGTTGAA+AGG + chr4_2:12062710-12062729 Msa0589360:CDS 40.0%
TCCTTTCAACAGGATCAACA+AGG - chr4_2:12062714-12062733 None:intergenic 40.0%
CCAATAGTGTTCCTTTCAAC+AGG - chr4_2:12062724-12062743 None:intergenic 40.0%
CCTGTTGAAAGGAACACTAT+TGG + chr4_2:12062721-12062740 Msa0589360:CDS 40.0%
AGATTCAGAGCTGATAATCC+AGG + chr4_2:12062772-12062791 Msa0589360:CDS 40.0%
GTTCGACCTGTTGAATTGAT+CGG - chr4_2:12062843-12062862 None:intergenic 40.0%
GATCAAATCAACAGGCCAAA+TGG - chr4_2:12062871-12062890 None:intergenic 40.0%
AAACAAAACAACGGTTCACC+CGG - chr4_2:12062940-12062959 None:intergenic 40.0%
! CAATGATCCTGAGGTTTTAC+TGG + chr4_2:12063038-12063057 Msa0589360:intron 40.0%
TTGGTTCATGCATTGCCATT+TGG + chr4_2:12063217-12063236 Msa0589360:CDS 40.0%
ATGTTGTATGCACCTCCAAA+TGG - chr4_2:12063235-12063254 None:intergenic 40.0%
! ATTTGGAGGTGCATACAACA+TGG + chr4_2:12063234-12063253 Msa0589360:CDS 40.0%
! TTTGGAGGTGCATACAACAT+GGG + chr4_2:12063235-12063254 Msa0589360:CDS 40.0%
TACAACATGGGGTCTAAAGA+TGG + chr4_2:12063247-12063266 Msa0589360:CDS 40.0%
! GGGTCTAAAGATGGCATTTT+TGG + chr4_2:12063256-12063275 Msa0589360:CDS 40.0%
!!! ATGGCATTTTTGGTGGACAA+TGG + chr4_2:12063266-12063285 Msa0589360:CDS 40.0%
!! TTTTTGGTGGACAATGGCAA+AGG + chr4_2:12063272-12063291 Msa0589360:CDS 40.0%
! GGAGCTATCACTGATTGTTT+AGG - chr4_2:12063299-12063318 None:intergenic 40.0%
ATTCACAAAAGTAGGTGTGG+AGG + chr4_2:12063390-12063409 Msa0589360:three_prime_UTR 40.0%
TTCACAAAAGTAGGTGTGGA+GGG + chr4_2:12063391-12063410 Msa0589360:three_prime_UTR 40.0%
CTTTGGTCGAGTGCAAAGTT+AGG + chr4_2:12059213-12059232 Msa0589360:CDS 45.0%
!! AAATCTTAAAGAGTGCCCCG+GGG - chr4_2:12059461-12059480 None:intergenic 45.0%
!! GAAATCTTAAAGAGTGCCCC+GGG - chr4_2:12059462-12059481 None:intergenic 45.0%
!! AAATCTTAAAGAGTGCCCCG+GGG - chr4_2:12059461-12059480 None:intergenic 45.0%
!! GAAATCTTAAAGAGTGCCCC+GGG - chr4_2:12059462-12059481 None:intergenic 45.0%
GCATGTGTGGTTGGAAGAAA+AGG - chr4_2:12059910-12059929 None:intergenic 45.0%
CACAGTTAATGGGAAGTTCC+CGG + chr4_2:12060069-12060088 Msa0589360:CDS 45.0%
ACAGTTAATGGGAAGTTCCC+GGG + chr4_2:12060070-12060089 Msa0589360:CDS 45.0%
TATCACTGTATCATCCTCCC+TGG - chr4_2:12060110-12060129 None:intergenic 45.0%
GGCATGGTATTAGGCAATTG+AGG + chr4_2:12060592-12060611 Msa0589360:intron 45.0%
GGTATTAGGCAATTGAGGAC+AGG + chr4_2:12060597-12060616 Msa0589360:CDS 45.0%
TTAGGCAATTGAGGACAGGT+TGG + chr4_2:12060601-12060620 Msa0589360:CDS 45.0%
TAGGCAATTGAGGACAGGTT+GGG + chr4_2:12060602-12060621 Msa0589360:CDS 45.0%
GGACACTGTGTGATATATGC+AGG - chr4_2:12060636-12060655 None:intergenic 45.0%
ACACAGTGTCCAATTCAACC+AGG + chr4_2:12060645-12060664 Msa0589360:CDS 45.0%
TGGCTAAGATCAACTGTCCA+TGG + chr4_2:12060738-12060757 Msa0589360:CDS 45.0%
!! TCTGGCTTTGGGAAAGGATA+AGG - chr4_2:12060795-12060814 None:intergenic 45.0%
!! TCATCATCTGGCTTTGGGAA+AGG - chr4_2:12060801-12060820 None:intergenic 45.0%
CCCAAAGCCAGATGATGAAT+TGG + chr4_2:12060803-12060822 Msa0589360:CDS 45.0%
TGTCCCAGGAAGACCATTTA+TGG - chr4_2:12061192-12061211 None:intergenic 45.0%
GTGGCCAATTGTTCTACACA+AGG + chr4_2:12061212-12061231 Msa0589360:CDS 45.0%
TACAAACTACCTGTGGAGAG+TGG + chr4_2:12061869-12061888 Msa0589360:CDS 45.0%
GCAACAGGTGCATCCATAAA+AGG - chr4_2:12062103-12062122 None:intergenic 45.0%
GCAGCTTTACATGAAGGACA+AGG - chr4_2:12062343-12062362 None:intergenic 45.0%
AGATGAACAGGATGGTTCTC+AGG - chr4_2:12062616-12062635 None:intergenic 45.0%
AACACTATTGGTGTACCAGC+TGG + chr4_2:12062733-12062752 Msa0589360:CDS 45.0%
ATCAACAGGCCAAATGGCTA+TGG - chr4_2:12062865-12062884 None:intergenic 45.0%
! GTTCATGCATTGCCATTTGG+AGG + chr4_2:12063220-12063239 Msa0589360:CDS 45.0%
! TTGGAGGTGCATACAACATG+GGG + chr4_2:12063236-12063255 Msa0589360:CDS 45.0%
!!! AGTTTTTTTTTTTTTTTTAA+GGG - chr4_2:12060410-12060429 None:intergenic 5.0%
!!! AAGTTTTTTTTTTTTTTTTA+AGG - chr4_2:12060411-12060430 None:intergenic 5.0%
ACTTTGCACTCGACCAAAGC+TGG - chr4_2:12059212-12059231 None:intergenic 50.0%
!! AATCTTAAAGAGTGCCCCGG+GGG - chr4_2:12059460-12059479 None:intergenic 50.0%
!! AATCTTAAAGAGTGCCCCGG+GGG - chr4_2:12059460-12059479 None:intergenic 50.0%
TCCTCCCTGGCATAAAGAGT+GGG - chr4_2:12060097-12060116 None:intergenic 50.0%
ATCCTCCCTGGCATAAAGAG+TGG - chr4_2:12060098-12060117 None:intergenic 50.0%
AAATGGTCTTCCTGGGACAG+TGG + chr4_2:12061193-12061212 Msa0589360:CDS 50.0%
GGACAGTCACTCAAACGTAG+TGG + chr4_2:12061579-12061598 Msa0589360:intron 50.0%
! TAGGTTTTGCCACTCTCCAC+AGG - chr4_2:12061881-12061900 None:intergenic 50.0%
ACTATTGGTGTACCAGCTGG+TGG + chr4_2:12062736-12062755 Msa0589360:CDS 50.0%
CAGGTCGAACCATAGCCATT+TGG + chr4_2:12062853-12062872 Msa0589360:intron 50.0%
GGCTGAACCAGTAAAACCTC+AGG - chr4_2:12063048-12063067 None:intergenic 50.0%
GTCCTTAACTAGTGTCCCGG+AGG + chr4_2:12059623-12059642 Msa0589360:intron 55.0%
TGCCTCCGGGACACTAGTTA+AGG - chr4_2:12059628-12059647 None:intergenic 55.0%
TGGCATAAAGAGTGGGTCCC+GGG - chr4_2:12060090-12060109 None:intergenic 55.0%
CTGGCATAAAGAGTGGGTCC+CGG - chr4_2:12060091-12060110 None:intergenic 55.0%
GGGACCCACTCTTTATGCCA+GGG + chr4_2:12060090-12060109 Msa0589360:CDS 55.0%
ACCCACTCTTTATGCCAGGG+AGG + chr4_2:12060093-12060112 Msa0589360:CDS 55.0%
! TTGAGGACAGGTTGGGCTGA+TGG + chr4_2:12060609-12060628 Msa0589360:CDS 55.0%
GAACAATTGGCCACTGTCCC+AGG - chr4_2:12061206-12061225 None:intergenic 55.0%
TTGGTGTACCAGCTGGTGGT+TGG + chr4_2:12062740-12062759 Msa0589360:CDS 55.0%
TGGTGTACCAGCTGGTGGTT+GGG + chr4_2:12062741-12062760 Msa0589360:CDS 55.0%
ATTGCAACCCAACCACCAGC+TGG - chr4_2:12062751-12062770 None:intergenic 55.0%
GTAGGTGTGGAGGGATACAG+AGG + chr4_2:12063400-12063419 Msa0589360:three_prime_UTR 55.0%
! GCCTTGTTAACGAGTGCCCC+CGG + chr4_2:12059441-12059460 Msa0589360:intron 60.0%
CCTTGTTAACGAGTGCCCCC+GGG + chr4_2:12059442-12059461 Msa0589360:intron 60.0%
CTTGTTAACGAGTGCCCCCG+GGG + chr4_2:12059443-12059462 Msa0589360:intron 60.0%
! GCCTTGTTAACGAGTGCCCC+CGG + chr4_2:12059441-12059460 Msa0589360:intron 60.0%
CCTTGTTAACGAGTGCCCCC+GGG + chr4_2:12059442-12059461 Msa0589360:intron 60.0%
CTTGTTAACGAGTGCCCCCG+GGG + chr4_2:12059443-12059462 Msa0589360:intron 60.0%
CGGGACCCACTCTTTATGCC+AGG + chr4_2:12060089-12060108 Msa0589360:CDS 60.0%
TGGAGGGATACAGAGGAGCC+AGG + chr4_2:12063407-12063426 Msa0589360:three_prime_UTR 60.0%
!! CCCGGGGGCACTCGTTAACA+AGG - chr4_2:12059445-12059464 None:intergenic 65.0%
!! CCCGGGGGCACTCGTTAACA+AGG - chr4_2:12059445-12059464 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 12059078 12063767 12059078 ID=Msa0589360;Name=Msa0589360
chr4_2 mRNA 12059078 12063767 12059078 ID=Msa0589360-mRNA-1;Parent=Msa0589360;Name=Msa0589360-mRNA-1;_AED=0.03;_eAED=0.03;_QI=86|1|1|1|1|1|6|424|557
chr4_2 exon 12059078 12059250 12059078 ID=Msa0589360-mRNA-1:exon:3553;Parent=Msa0589360-mRNA-1
chr4_2 exon 12060022 12060173 12060022 ID=Msa0589360-mRNA-1:exon:3554;Parent=Msa0589360-mRNA-1
chr4_2 exon 12060593 12060837 12060593 ID=Msa0589360-mRNA-1:exon:3555;Parent=Msa0589360-mRNA-1
chr4_2 exon 12061105 12061233 12061105 ID=Msa0589360-mRNA-1:exon:3556;Parent=Msa0589360-mRNA-1
chr4_2 exon 12061864 12062793 12061864 ID=Msa0589360-mRNA-1:exon:3557;Parent=Msa0589360-mRNA-1
chr4_2 exon 12063213 12063767 12063213 ID=Msa0589360-mRNA-1:exon:3558;Parent=Msa0589360-mRNA-1
chr4_2 five_prime_UTR 12059078 12059163 12059078 ID=Msa0589360-mRNA-1:five_prime_utr;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12059164 12059250 12059164 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12060022 12060173 12060022 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12060593 12060837 12060593 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12061105 12061233 12061105 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12061864 12062793 12061864 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 CDS 12063213 12063343 12063213 ID=Msa0589360-mRNA-1:cds;Parent=Msa0589360-mRNA-1
chr4_2 three_prime_UTR 12063344 12063767 12063344 ID=Msa0589360-mRNA-1:three_prime_utr;Parent=Msa0589360-mRNA-1
Gene Sequence

>Msa0589360

ATGGTTTCTTCGATTCGAGTACTACTTCTTATTTGTTGCATATTTCCAGCTTTGGTCGAGTGCAAAGTTAGGCACTACAAATTTCATGTGGTGGCCAAAAATACAACTAGATTATGTTCAAGCAAAGCAATTGTCACAGTTAATGGGAAGTTCCCGGGACCCACTCTTTATGCCAGGGAGGATGATACAGTGATAGTCAAAGTTCGTAATCAAGTCAACCACAATATTACCATCCATTGGCATGGTATTAGGCAATTGAGGACAGGTTGGGCTGATGGACCTGCATATATCACACAGTGTCCAATTCAACCAGGACATAGTTATACATACAATTTCACCATTACTGGTCAAAGAGGAACACTTCTTTGGCATGCACATGTTAATTGGCTAAGATCAACTGTCCATGGTGCCATAGTTATTTTACCAAAGAAAGGTGTTCCTTATCCTTTCCCAAAGCCAGATGATGAATTGGTTTTGGTATTAGGAGAGTGGTGGAAATCAGATACTGAAGCTGTTATCAATGAAGCTCTAAAATCAGGAATGGCACCAAATGTTTCAGATGCTCATACCATAAATGGTCTTCCTGGGACAGTGGCCAATTGTTCTACACAAGATGTTTACAAACTACCTGTGGAGAGTGGCAAAACCTACCTACTAAGAATCATCAATGCTGCACTCAATGAAGAACTTTTCTTCAAAATAGCTGGCCACAAACTCACAGTAGTAGAAGTTGATGCAACATACACAAAACCATTCCAAATTGAAACAATTGTGATAGCACCTGGTCAAACAACAAATGTACTATTAAAAGCAAACCAAAAATCTGGAAAATATTTAGTAGCTGCTTCACCTTTTATGGATGCACCTGTTGCTGTAGACAATTTAACAGCCACAGCTACATTACATTATTCAGGAACTACTCTTACAAACACCCCTACATTTCTAACTACACCTCCACCTAAAAATGCTACACAAATTGCTAATAATTTTTTAAACTCACTTAAAGGACTTAATTCCAAGAAATACCCAGTTAATGTTCCTTTAAAAATTGATCATTCACTTTTTTTTACTGTTGGATTAGGGGTTAATCCTTGTCCTTCATGTAAAGCTGCAAATGGTAGTAGAGTTGTAGCTGCTATTAATAATGTTACATTTGTTATGCCAACTACAGCTTTACTACAAGCACATTATTTCAACATTAAAAATGTTTTTACTACAGATTTTCCACCAAATCCACCTCATATTTATAATTTCACTGGTGCTGGACCTAAAAACTTGAACACTACAAGTGGTACAAAGTTATATAAGTTATCATTTAATGATACTGTTCAGCTTGTTATGCAAGATACTGGAATCATTGCACCTGAGAACCATCCTGTTCATCTTCATGGTTTCAATTTCTTTGTTGTTGGTAAAGGAGTTGGAAATTATGATAGTAAAAATGATCCAAAGAAGTTTAACCTTGTTGATCCTGTTGAAAGGAACACTATTGGTGTACCAGCTGGTGGTTGGGTTGCAATTAGATTCAGAGCTGATAATCCAGGAGTTTGGTTCATGCATTGCCATTTGGAGGTGCATACAACATGGGGTCTAAAGATGGCATTTTTGGTGGACAATGGCAAAGGTCCTAAACAATCAGTGATAGCTCCACCAAAAGATCTTCCAAAATGTTGA
Protein sequence

>Msa0589360

MVSSIRVLLLICCIFPALVECKVRHYKFHVVAKNTTRLCSSKAIVTVNGKFPGPTLYAREDDTVIVKVRNQVNHNITIHWHGIRQLRTGWADGPAYITQCPIQPGHSYTYNFTITGQRGTLLWHAHVNWLRSTVHGAIVILPKKGVPYPFPKPDDELVLVLGEWWKSDTEAVINEALKSGMAPNVSDAHTINGLPGTVANCSTQDVYKLPVESGKTYLLRIINAALNEELFFKIAGHKLTVVEVDATYTKPFQIETIVIAPGQTTNVLLKANQKSGKYLVAASPFMDAPVAVDNLTATATLHYSGTTLTNTPTFLTTPPPKNATQIANNFLNSLKGLNSKKYPVNVPLKIDHSLFFTVGLGVNPCPSCKAANGSRVVAAINNVTFVMPTTALLQAHYFNIKNVFTTDFPPNPPHIYNFTGAGPKNLNTTSGTKLYKLSFNDTVQLVMQDTGIIAPENHPVHLHGFNFFVVGKGVGNYDSKNDPKKFNLVDPVERNTIGVPAGGWVAIRFRADNPGVWFMHCHLEVHTTWGLKMAFLVDNGKGPKQSVIAPPKDLPKC*