AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0546690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546690 MtrunA17_Chr4g0028471 99.661 590 2 0 1 590 1 590 0.0 1220
Msa0546690 MtrunA17_Chr2g0327291 89.322 590 63 0 1 590 1 590 0.0 1082
Msa0546690 MtrunA17_Chr3g0119921 43.382 544 283 6 62 584 173 712 5.98e-156 464
Msa0546690 MtrunA17_Chr7g0220881 40.179 560 290 6 62 582 152 705 6.79e-144 433
Msa0546690 MtrunA17_Chr8g0371361 41.547 556 286 8 62 582 152 703 1.96e-143 432
Msa0546690 MtrunA17_Chr1g0181461 41.667 552 285 9 66 582 2 551 1.59e-141 422
Msa0546690 MtrunA17_Chr3g0129301 29.202 589 386 9 11 574 50 632 4.32e-70 239
Msa0546690 MtrunA17_Chr3g0129301 26.316 247 149 4 64 306 433 650 1.46e-19 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0546690 AT5G60790.1 84.409 558 87 0 33 590 38 595 0.0 1008
Msa0546690 AT1G64550.1 44.404 545 277 6 62 584 173 713 4.05e-158 470
Msa0546690 AT3G54540.2 42.602 561 276 9 62 583 161 714 4.54e-152 454
Msa0546690 AT3G54540.1 42.602 561 276 9 62 583 161 714 4.54e-152 454
Msa0546690 AT5G64840.1 31.273 534 345 7 60 574 94 624 6.33e-75 252
Msa0546690 AT5G64840.1 25.311 241 143 3 64 298 425 634 2.37e-17 86.3
Msa0546690 AT5G09930.1 31.047 554 353 8 46 578 69 614 1.43e-73 248
Msa0546690 AT5G09930.1 25.726 241 134 7 64 294 411 616 1.71e-15 80.5
Msa0546690 AT3G28860.1 28.856 201 128 6 81 276 1030 1220 1.76e-11 67.8
Msa0546690 AT1G28010.1 30.342 234 123 8 375 581 1006 1226 8.98e-11 65.9

Find 205 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCCCAGGATTTCCTTAA+TGG 0.155402 4_1:+38889264 Msa0546690:CDS
GATAGAATAAGCATCATTTA+AGG 0.186560 4_1:-38890603 None:intergenic
TATGGTTTACTTGGATTAAA+TGG 0.214199 4_1:+38887650 Msa0546690:CDS
GAAGAAGTTTGAACGTATTT+TGG 0.216007 4_1:+38889230 Msa0546690:CDS
TCAGCACTTGGCTGAGAAAT+TGG 0.236239 4_1:+38889812 Msa0546690:CDS
TCTGAATTGGAGTTAAATTA+TGG 0.236842 4_1:+38887498 Msa0546690:CDS
ATCTTCTCTTCCTCATTTCC+AGG 0.257833 4_1:-38889876 None:intergenic
GTATTATTATCAGTTTCTTT+TGG 0.264207 4_1:+38890670 Msa0546690:three_prime_UTR
ATCCTCTTTCTAGAGATATT+CGG 0.289431 4_1:+38886593 Msa0546690:CDS
CGAACGAAGGGTTGAGATTA+GGG 0.290104 4_1:-38886308 None:intergenic
CAAATGGGAGGGTGACATTA+TGG 0.294052 4_1:+38890187 Msa0546690:CDS
AATCAGGTTTGCTTCGATAT+TGG 0.295033 4_1:+38890336 Msa0546690:three_prime_UTR
ATCAGGTGGCGCATGAGATA+TGG 0.300121 4_1:+38890141 Msa0546690:CDS
AGGGCTGCTATTGGGAGGTT+TGG 0.309698 4_1:+38889900 Msa0546690:CDS
TCCTCTTTCTAGAGATATTC+GGG 0.322786 4_1:+38886594 Msa0546690:CDS
CCGAACGAAGGGTTGAGATT+AGG 0.322913 4_1:-38886309 None:intergenic
GAAGACGAAGGCGGGGATAT+TGG 0.323597 4_1:+38890226 Msa0546690:CDS
AAGGAATATATTGCTCGATT+TGG 0.324399 4_1:+38889432 Msa0546690:CDS
AGCTGAAACTTTGGCTGCTC+AGG 0.334938 4_1:+38887835 Msa0546690:CDS
ACAGCCTCAATTGCTACTTC+TGG 0.335559 4_1:+38890010 Msa0546690:CDS
TGACTTTAGGCTCATTAATC+AGG 0.343599 4_1:+38890124 Msa0546690:CDS
AATCAGTGATATGAAGATTT+CGG 0.349665 4_1:+38886540 Msa0546690:CDS
CACCACCCTTTGGAGACTTA+TGG 0.350545 4_1:+38890286 Msa0546690:three_prime_UTR
CCCACTTGTACTGCTTCATC+TGG 0.353903 4_1:-38889386 None:intergenic
CAAAACTTACTTCAGAAAAC+TGG 0.355736 4_1:-38889605 None:intergenic
AGCGACCTTATCAGCTGCTT+TGG 0.357140 4_1:-38886512 None:intergenic
TCAGGTTTATCTAATAAATC+AGG 0.358920 4_1:+38890320 Msa0546690:three_prime_UTR
TATTTGTGCAGACACCATTA+AGG 0.359994 4_1:-38889278 None:intergenic
CATCATTTAAGGTTCAGTTT+AGG 0.369029 4_1:-38890592 None:intergenic
GAAGTTCTTAGCTAGTATTA+TGG 0.369516 4_1:-38890365 None:intergenic
GAGAAGATGAGGGCTGCTAT+TGG 0.370014 4_1:+38889891 Msa0546690:CDS
TGAACGTATTTATGAACGTT+TGG 0.370645 4_1:+38887967 Msa0546690:CDS
GTGCTTATTATTTAATTGCT+TGG 0.375910 4_1:+38890538 Msa0546690:three_prime_UTR
ATTGCAGATAGAGTCTTTAT+CGG 0.376053 4_1:+38887443 Msa0546690:intron
GGATGAGCCAACTAATCATT+TGG 0.377600 4_1:+38890031 Msa0546690:CDS
TGCTTGTTAGCCATGACTTT+AGG 0.381908 4_1:+38890111 Msa0546690:CDS
TCTACCCTGCTTACTGCAAT+AGG 0.386530 4_1:+38887683 Msa0546690:CDS
CCATCAGACAAATTCTTCAT+AGG 0.387311 4_1:-38889948 None:intergenic
TCTCATTGTTGATTCTGAAT+TGG 0.394499 4_1:+38887485 Msa0546690:CDS
TTTGGTATGGAATATATATA+TGG 0.395130 4_1:-38886245 None:intergenic
TTGACCAGCAAGATGGATAA+TGG 0.395924 4_1:+38890564 Msa0546690:three_prime_UTR
GTACTCTTTCCAGCACCATT+TGG 0.396145 4_1:-38889705 None:intergenic
GCTGAAATCTTAGATGGTCT+TGG 0.397956 4_1:+38888025 Msa0546690:CDS
TTTCTGCGGTTGATGCATCT+AGG 0.401162 4_1:-38887994 None:intergenic
CTTATGGCCTTGCTTACATC+AGG 0.402681 4_1:+38890302 Msa0546690:three_prime_UTR
AGCTGTTAGTGATGAGATAT+AGG 0.402720 4_1:+38890254 Msa0546690:three_prime_UTR
TGGATATTCATCACCTTAGC+AGG 0.407935 4_1:+38887735 Msa0546690:CDS
TCCAGACGTTATGGTTTACT+TGG 0.408460 4_1:+38887641 Msa0546690:intron
AAGGGTTGAGATTAGGGTTG+TGG 0.409947 4_1:-38886302 None:intergenic
GGAGAAAGAAGCTGAAACTT+TGG 0.410674 4_1:+38887826 Msa0546690:CDS
GGAGGCACCCACCAAGATAT+TGG 0.419291 4_1:-38890395 None:intergenic
CTTGGCTGAGAAATTGGACT+TGG 0.419614 4_1:+38889818 Msa0546690:CDS
GGAGTATGAGTGGTGAAGTT+TGG 0.423952 4_1:-38886263 None:intergenic
TAAATTTGGTTACAGGATGA+TGG 0.428939 4_1:+38887929 Msa0546690:intron
AGAAGATGAGGGCTGCTATT+GGG 0.429033 4_1:+38889892 Msa0546690:CDS
TCAATATCCAAATGATTAGT+TGG 0.429150 4_1:-38890038 None:intergenic
CATAATGTCACCCTCCCATT+TGG 0.429695 4_1:-38890186 None:intergenic
CTTAGACTCTAGGATTGCTC+TGG 0.432478 4_1:+38889677 Msa0546690:CDS
ACTTCAGAAAACTGGAGAAC+AGG 0.434793 4_1:-38889597 None:intergenic
TGTCCCATTATCCATCTTGC+TGG 0.442873 4_1:-38890568 None:intergenic
TTACTTGGATTAAATGGTTG+TGG 0.447320 4_1:+38887656 Msa0546690:CDS
AAGTTACCAAGATGATTAGT+TGG 0.447423 4_1:-38888163 None:intergenic
TATCATAAATATAGTATTGT+CGG 0.449438 4_1:-38890637 None:intergenic
GACTCTAGGATTGCTCTGGT+TGG 0.451696 4_1:+38889681 Msa0546690:CDS
GGCCTTACCACCACGTTTAG+CGG 0.454166 4_1:-38886473 None:intergenic
TGACCAGCAAGATGGATAAT+GGG 0.454508 4_1:+38890565 Msa0546690:three_prime_UTR
ATTTCGGATCGGACTTGTAC+TGG 0.457445 4_1:+38886556 Msa0546690:CDS
AAGCATCTGAAGACGAAGGC+GGG 0.463027 4_1:+38890218 Msa0546690:CDS
GGTTGTGATATCACATTCCC+AGG 0.464036 4_1:+38889251 Msa0546690:CDS
ACCACGTTTAGCGGCGGCGG+CGG 0.465477 4_1:-38886464 None:intergenic
CTTCAGTACAACCAATATCT+TGG 0.465681 4_1:+38890384 Msa0546690:three_prime_UTR
AACGTTCATAAATACGTTCA+AGG 0.466725 4_1:-38887964 None:intergenic
TTCTTCCAAAGCAGCTGATA+AGG 0.468343 4_1:+38886507 Msa0546690:CDS
GCATTCCTATTGCAGTAAGC+AGG 0.469207 4_1:-38887688 None:intergenic
TTAGGGTTGTGGTTTGAAAG+AGG 0.473496 4_1:-38886291 None:intergenic
CCTAATCTCAACCCTTCGTT+CGG 0.474647 4_1:+38886309 Msa0546690:five_prime_UTR
GGATAGAAACCACCACCCTT+TGG 0.475124 4_1:+38890276 Msa0546690:three_prime_UTR
TCAGGTGGCGCATGAGATAT+GGG 0.475280 4_1:+38890142 Msa0546690:CDS
TGATGAAGAAAGGTTGCAAT+TGG 0.476798 4_1:+38887805 Msa0546690:CDS
TATTGAGACAATTGACTCCT+TGG 0.477061 4_1:+38890055 Msa0546690:CDS
AGACACCATTAAGGAAATCC+TGG 0.479174 4_1:-38889269 None:intergenic
CTGGTTGGACCAAATGGTGC+TGG 0.479286 4_1:+38889696 Msa0546690:CDS
TTGCAACCTTTCTTCATCAC+AGG 0.480984 4_1:-38887801 None:intergenic
TTCTGCGGTTGATGCATCTA+GGG 0.481859 4_1:-38887993 None:intergenic
TTGTGTTTGAATTTCGAAGA+TGG 0.481860 4_1:+38886402 Msa0546690:exon
GAAGCAGTACAAGTGGGAAC+AGG 0.482565 4_1:+38889392 Msa0546690:CDS
ATCCCACTCATTCAATGCCT+CGG 0.483892 4_1:-38890078 None:intergenic
ATTGGGAGGTTTGGACTGAC+AGG 0.484846 4_1:+38889909 Msa0546690:CDS
AGTATTCCGCTTCACTGATG+TGG 0.487881 4_1:+38889560 Msa0546690:CDS
TCTTCTCTTCCTCATTTCCA+GGG 0.489690 4_1:-38889875 None:intergenic
CGTGCTGCTGAAATCTTAGA+TGG 0.490419 4_1:+38888019 Msa0546690:CDS
ACCACCACGTTTAGCGGCGG+CGG 0.490492 4_1:-38886467 None:intergenic
GATATTGAGCAATTCGAAGA+TGG 0.490587 4_1:-38889788 None:intergenic
CTGATCAGAAAGTGACCAAA+TGG 0.491350 4_1:+38890171 Msa0546690:CDS
CGCCGCTAAACGTGGTGGTA+AGG 0.493469 4_1:+38886471 Msa0546690:CDS
TTGTTTGGATTGAGATAACT+TGG 0.496182 4_1:+38890477 Msa0546690:three_prime_UTR
GGTGATTTGTTTCCTAGTGA+TGG 0.498265 4_1:+38889747 Msa0546690:CDS
TTGGGGTTGACTTAGACTCT+AGG 0.501919 4_1:+38889667 Msa0546690:CDS
GATGAACCAACTAATCATCT+TGG 0.503837 4_1:+38888157 Msa0546690:CDS
ATTGCTCTGGTTGGACCAAA+TGG 0.504021 4_1:+38889690 Msa0546690:CDS
AGGCTTCAATTTCCCTGCTA+AGG 0.505225 4_1:-38887748 None:intergenic
AGAAAACTGGAGAACAGGAG+GGG 0.505371 4_1:-38889592 None:intergenic
ATATCCATGTGCTCAGGAAT+CGG 0.505823 4_1:-38887719 None:intergenic
TTTGGAAGAAGCAGCGGCTG+CGG 0.507914 4_1:-38886494 None:intergenic
TTCAGCAAAACTAGCTCGCC+AGG 0.509474 4_1:+38889461 Msa0546690:CDS
AGTGAGAGAGAAGATAGATC+TGG 0.513413 4_1:-38886361 None:intergenic
TGTTCAGACTCGTGGTGACT+TGG 0.515232 4_1:+38889356 Msa0546690:CDS
ATGAGTGGTGAAGTTTGGTA+TGG 0.520507 4_1:-38886258 None:intergenic
AGCCTCTGACATGTCTGCAT+TGG 0.520639 4_1:+38887766 Msa0546690:CDS
TGTGGTTTGAAAGAGGAAGA+TGG 0.522076 4_1:-38886284 None:intergenic
ACCCGAATATCTCTAGAAAG+AGG 0.522321 4_1:-38886595 None:intergenic
CTCATCATAAACTGAAGGGC+AGG 0.530872 4_1:-38889846 None:intergenic
GCTGTTAGTGATGAGATATA+GGG 0.531494 4_1:+38890255 Msa0546690:three_prime_UTR
TATATTGCTCGATTTGGTCA+TGG 0.531607 4_1:+38889438 Msa0546690:CDS
GAAAACTGGAGAACAGGAGG+GGG 0.533791 4_1:-38889591 None:intergenic
GACAATTGACTCCTTGGCCG+AGG 0.534952 4_1:+38890061 Msa0546690:CDS
TAAATAATAAGCACAACAAA+CGG 0.536780 4_1:-38890529 None:intergenic
GTATTCCGCTTCACTGATGT+GGG 0.537969 4_1:+38889561 Msa0546690:CDS
AGTCGGTTCCGATGAAAAGA+TGG 0.538929 4_1:-38886334 None:intergenic
AGCTTTCTTCTTGCTAGCGT+CGG 0.543158 4_1:-38886428 None:intergenic
TGATGAATATCCATGTGCTC+AGG 0.545545 4_1:-38887725 None:intergenic
AATTCTTCATAGGCATCACC+TGG 0.549166 4_1:-38889938 None:intergenic
GCTTACTGCAATAGGAATGC+GGG 0.552380 4_1:+38887691 Msa0546690:CDS
TCAGCTTCTTACTTTGCATG+TGG 0.552733 4_1:-38889305 None:intergenic
AGCTCGCCAGGCACAGAGCA+AGG 0.552992 4_1:+38889473 Msa0546690:CDS
CGCTTGACCATGCCATCACT+AGG 0.553023 4_1:-38889759 None:intergenic
GTTGAGTTCAATGGCAGAGT+CGG 0.555106 4_1:+38890440 Msa0546690:three_prime_UTR
ATTCACTCATCATAAACTGA+AGG 0.557664 4_1:-38889851 None:intergenic
TTTGTTTCCTAGTGATGGCA+TGG 0.558297 4_1:+38889752 Msa0546690:CDS
CAGTACAACCAATATCTTGG+TGG 0.560812 4_1:+38890387 Msa0546690:three_prime_UTR
TTGGTTCTTGACCAGCAAGA+TGG 0.561244 4_1:+38890557 Msa0546690:three_prime_UTR
AGCAATATATTCCTTCATGT+TGG 0.561912 4_1:-38889424 None:intergenic
AATTGGAGTTAAATTATGGA+AGG 0.564752 4_1:+38887502 Msa0546690:CDS
CAGAAAACTGGAGAACAGGA+GGG 0.565180 4_1:-38889593 None:intergenic
AAATTCAAACACAAACCCTA+AGG 0.565291 4_1:-38886393 None:intergenic
TCATAACCTGTGATGAAGAA+AGG 0.568129 4_1:+38887795 Msa0546690:CDS
AAGATAGATCTGGCCTAAGT+CGG 0.568371 4_1:-38886351 None:intergenic
TCATCCAGAAGTAGCAATTG+AGG 0.569015 4_1:-38890014 None:intergenic
ACGTTCATAAATACGTTCAA+GGG 0.570004 4_1:-38887963 None:intergenic
TGGTTCATCGAGTAGAAGAA+TGG 0.570541 4_1:-38888143 None:intergenic
TCATCGAGTAGAAGAATGGT+GGG 0.571360 4_1:-38888139 None:intergenic
GAAAAGATGGAGCCGAACGA+AGG 0.573743 4_1:-38886321 None:intergenic
CTCATTCAATGCCTCGGCCA+AGG 0.573903 4_1:-38890072 None:intergenic
TGCTCAATATCATCAGCACT+TGG 0.575155 4_1:+38889800 Msa0546690:CDS
CTATGAAGAATTTGTCTGAT+GGG 0.576561 4_1:+38889949 Msa0546690:CDS
CAAGGCCATAAGTCTCCAAA+GGG 0.577796 4_1:-38890291 None:intergenic
AAAAGATGGAGCCGAACGAA+GGG 0.584128 4_1:-38886320 None:intergenic
GTGGTGATTGTTGAGTTCAA+TGG 0.585033 4_1:+38890431 Msa0546690:three_prime_UTR
TGCTTACTGCAATAGGAATG+CGG 0.585830 4_1:+38887690 Msa0546690:CDS
TGGCCGAGGCATTGAATGAG+TGG 0.593068 4_1:+38890075 Msa0546690:CDS
GCAAGGCCATAAGTCTCCAA+AGG 0.593440 4_1:-38890292 None:intergenic
AGATAAACCTGATGTAAGCA+AGG 0.594800 4_1:-38890309 None:intergenic
GAAATGAGGAAGAGAAGATG+AGG 0.595660 4_1:+38889880 Msa0546690:CDS
GCCGCCGCCGCCGCTAAACG+TGG 0.595826 4_1:+38886463 Msa0546690:CDS
TGATCAGAAAGTGACCAAAT+GGG 0.596562 4_1:+38890172 Msa0546690:CDS
GACACCATTAAGGAAATCCT+GGG 0.599092 4_1:-38889268 None:intergenic
ACACTGCTGAAGCTAATGAC+AGG 0.601098 4_1:+38889726 Msa0546690:CDS
TGGTTACAGGATGATGGAGG+TGG 0.601235 4_1:+38887935 Msa0546690:intron
CATAAGTCTCCAAAGGGTGG+TGG 0.603246 4_1:-38890285 None:intergenic
CAGATTTGGAAGCTTGTGTG+TGG 0.605348 4_1:+38889193 Msa0546690:intron
GATTAATGAGCCTAAAGTCA+TGG 0.606985 4_1:-38890121 None:intergenic
ACTTCCGATTCCTGAGCACA+TGG 0.608657 4_1:+38887715 Msa0546690:CDS
GAGTGAATACCCTGGAAATG+AGG 0.608906 4_1:+38889866 Msa0546690:CDS
GGAGCAGATTGCCAACATGA+AGG 0.612931 4_1:+38889413 Msa0546690:CDS
ACCAGATGAAGCAGTACAAG+TGG 0.613873 4_1:+38889385 Msa0546690:CDS
TTTGGATTGAGATAACTTGG+TGG 0.614623 4_1:+38890480 Msa0546690:three_prime_UTR
AGCATCTGAAGACGAAGGCG+GGG 0.615979 4_1:+38890219 Msa0546690:CDS
CATTCCTATTGCAGTAAGCA+GGG 0.618085 4_1:-38887687 None:intergenic
AGAGGAAGATGGAGTATGAG+TGG 0.621019 4_1:-38886273 None:intergenic
AGAGTCGAGTAATCTTTGCA+TGG 0.621080 4_1:+38889976 Msa0546690:CDS
CCTATGAAGAATTTGTCTGA+TGG 0.621484 4_1:+38889948 Msa0546690:CDS
AATTCAAACACAAACCCTAA+GGG 0.621698 4_1:-38886392 None:intergenic
CTTTAGGCTCATTAATCAGG+TGG 0.621832 4_1:+38890127 Msa0546690:CDS
AGATGAGGGCTGCTATTGGG+AGG 0.621874 4_1:+38889895 Msa0546690:CDS
ATTTGGTTACAGGATGATGG+AGG 0.622194 4_1:+38887932 Msa0546690:intron
AGCTGCTTTGGAAGAAGCAG+CGG 0.624417 4_1:-38886500 None:intergenic
TGGACTGACAGGTAAAGCCC+AGG 0.625338 4_1:+38889920 Msa0546690:CDS
GAATGAGTGGGATGGCGGCA+TGG 0.625736 4_1:+38890088 Msa0546690:CDS
TCAGAAAACTGGAGAACAGG+AGG 0.625791 4_1:-38889594 None:intergenic
CAGAAAGTGACCAAATGGGA+GGG 0.626666 4_1:+38890176 Msa0546690:CDS
GAGGCATTGAATGAGTGGGA+TGG 0.626891 4_1:+38890080 Msa0546690:CDS
TTCACTCATCATAAACTGAA+GGG 0.629235 4_1:-38889850 None:intergenic
TTCATCGAGTAGAAGAATGG+TGG 0.635101 4_1:-38888140 None:intergenic
AGTACAACCAATATCTTGGT+GGG 0.637128 4_1:+38890388 Msa0546690:three_prime_UTR
AAGAAGCTGAAGATTTACAC+TGG 0.638565 4_1:+38889318 Msa0546690:CDS
GCCGCCGCCGCTAAACGTGG+TGG 0.638651 4_1:+38886466 Msa0546690:CDS
AAATGAGGAAGAGAAGATGA+GGG 0.639167 4_1:+38889881 Msa0546690:CDS
ACGAGGTGGACTCACAGAGA+AGG 0.639258 4_1:+38889518 Msa0546690:CDS
GTGATATGAAGATTTCGGAT+CGG 0.641156 4_1:+38886545 Msa0546690:CDS
GGCCGAGGCATTGAATGAGT+GGG 0.643694 4_1:+38890076 Msa0546690:CDS
GCATTGAATGAGTGGGATGG+CGG 0.643695 4_1:+38890083 Msa0546690:CDS
GATCAATATGTTCAGACTCG+TGG 0.643969 4_1:+38889348 Msa0546690:CDS
GAAGCATCTGAAGACGAAGG+CGG 0.649201 4_1:+38890217 Msa0546690:CDS
GGATATTCATCACCTTAGCA+GGG 0.652225 4_1:+38887736 Msa0546690:CDS
TCAGAAAGTGACCAAATGGG+AGG 0.653363 4_1:+38890175 Msa0546690:CDS
GGCCATAAGTCTCCAAAGGG+TGG 0.655589 4_1:-38890288 None:intergenic
TTCCAATGCAGACATGTCAG+AGG 0.661870 4_1:-38887768 None:intergenic
CTTACCACCACGTTTAGCGG+CGG 0.666853 4_1:-38886470 None:intergenic
AAAACTGGAGAACAGGAGGG+GGG 0.666914 4_1:-38889590 None:intergenic
CAAGAAGCATCTGAAGACGA+AGG 0.671286 4_1:+38890214 Msa0546690:CDS
TCAAACACAAACCCTAAGGG+AGG 0.672946 4_1:-38886389 None:intergenic
CCAGATGAAGCAGTACAAGT+GGG 0.690738 4_1:+38889386 Msa0546690:CDS
ATTCTTCATAGGCATCACCT+GGG 0.706152 4_1:-38889937 None:intergenic
CATCGAGTAGAAGAATGGTG+GGG 0.710127 4_1:-38888138 None:intergenic
TTTATGATGAGTGAATACCC+TGG 0.737237 4_1:+38889858 Msa0546690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAGTACATAATAAAATT+TGG - chr4_1:38887573-38887592 None:intergenic 10.0%
!! AGTTTATTTATTTATTTGTA+TGG + chr4_1:38887891-38887910 Msa0546690:intron 10.0%
!!! TTTTTTAATGATTTGAGTTA+TGG + chr4_1:38888560-38888579 Msa0546690:intron 15.0%
!! TTTGTAACAGATTATAAAAA+AGG - chr4_1:38888648-38888667 None:intergenic 15.0%
!!! TTTTTATAATCTGTTACAAA+CGG + chr4_1:38888648-38888667 Msa0546690:intron 15.0%
!! ATTACTTTGAATAATTTAGT+GGG + chr4_1:38888702-38888721 Msa0546690:intron 15.0%
!! TATCATAAATATAGTATTGT+CGG - chr4_1:38890640-38890659 None:intergenic 15.0%
!!! TATTATTATCAGTTTCTTTT+GGG + chr4_1:38890671-38890690 Msa0546690:three_prime_UTR 15.0%
!! TTTGGTATGGAATATATATA+TGG - chr4_1:38886248-38886267 None:intergenic 20.0%
!! CAGATTTATTGGTATAAATT+TGG + chr4_1:38886853-38886872 Msa0546690:intron 20.0%
!!! AAGTTGATATATGAATGAAT+CGG + chr4_1:38886925-38886944 Msa0546690:intron 20.0%
!! TATTTGACAACTTATTTAAC+CGG - chr4_1:38887007-38887026 None:intergenic 20.0%
!! AATGTGGAAATTATCTTAAT+TGG - chr4_1:38887065-38887084 None:intergenic 20.0%
!! AATTAAGATAATTTCCACAT+TGG + chr4_1:38887064-38887083 Msa0546690:intron 20.0%
!!! TATGTACTTTTATTTAAACC+TGG + chr4_1:38887581-38887600 Msa0546690:intron 20.0%
!! TTGCTAACTTTGAATAAATT+TGG + chr4_1:38887915-38887934 Msa0546690:intron 20.0%
!! ATAAACAAAGAAAAAAGACT+AGG - chr4_1:38888398-38888417 None:intergenic 20.0%
!!! ATTTTGTTCAAAAGGATTTA+GGG - chr4_1:38888674-38888693 None:intergenic 20.0%
!!! TATTTTGTTCAAAAGGATTT+AGG - chr4_1:38888675-38888694 None:intergenic 20.0%
!! CACTAAATTATTCAAAGTAA+TGG - chr4_1:38888703-38888722 None:intergenic 20.0%
!! CATTACTTTGAATAATTTAG+TGG + chr4_1:38888701-38888720 Msa0546690:intron 20.0%
!!! CACTATCATTTTATTTTATG+CGG + chr4_1:38888984-38889003 Msa0546690:intron 20.0%
!!! ACTATCATTTTATTTTATGC+GGG + chr4_1:38888985-38889004 Msa0546690:intron 20.0%
!!! TAGTTAGTTGATAAATGTAT+AGG - chr4_1:38889162-38889181 None:intergenic 20.0%
!! TAAATAATAAGCACAACAAA+CGG - chr4_1:38890532-38890551 None:intergenic 20.0%
!!! GTATTATTATCAGTTTCTTT+TGG + chr4_1:38890670-38890689 Msa0546690:three_prime_UTR 20.0%
! AATCAGTGATATGAAGATTT+CGG + chr4_1:38886540-38886559 Msa0546690:CDS 25.0%
!! TCTGAAGTTAATGATGATTA+TGG + chr4_1:38886723-38886742 Msa0546690:intron 25.0%
! AGTTAATGATGATTATGGAT+TGG + chr4_1:38886728-38886747 Msa0546690:intron 25.0%
! TGATCAATATGCAGATTTAT+TGG + chr4_1:38886842-38886861 Msa0546690:intron 25.0%
! TATAATTTCGTCATCATCAA+AGG - chr4_1:38886954-38886973 None:intergenic 25.0%
!! ACTTAATAATTTTGCCCTTT+TGG + chr4_1:38887039-38887058 Msa0546690:intron 25.0%
! ATTATCTTAATTGGCCAAAA+GGG - chr4_1:38887056-38887075 None:intergenic 25.0%
! AATTATCTTAATTGGCCAAA+AGG - chr4_1:38887057-38887076 None:intergenic 25.0%
!! TTAGTTTATTGTTGTTAGCA+TGG + chr4_1:38887219-38887238 Msa0546690:intron 25.0%
!! TAGTTTATTGTTGTTAGCAT+GGG + chr4_1:38887220-38887239 Msa0546690:intron 25.0%
! TCTGAATTGGAGTTAAATTA+TGG + chr4_1:38887498-38887517 Msa0546690:CDS 25.0%
! AATTGGAGTTAAATTATGGA+AGG + chr4_1:38887502-38887521 Msa0546690:CDS 25.0%
! TAATAAAATTTGGCTGTACT+CGG - chr4_1:38887563-38887582 None:intergenic 25.0%
! TATGGTTTACTTGGATTAAA+TGG + chr4_1:38887650-38887669 Msa0546690:CDS 25.0%
! CTTTGAATAAATTTGGTTAC+AGG + chr4_1:38887922-38887941 Msa0546690:intron 25.0%
! ATAAATTTCAGACAAACAAG+AGG - chr4_1:38888208-38888227 None:intergenic 25.0%
! GTAAAAAACAGAGTAACAAA+TGG - chr4_1:38888454-38888473 None:intergenic 25.0%
!! GGCAAAATATTTTGTTCAAA+AGG - chr4_1:38888682-38888701 None:intergenic 25.0%
!!! GCTTTAACTATGTCTTTTTT+TGG + chr4_1:38888841-38888860 Msa0546690:intron 25.0%
!!! ATGCTTTACTATGTCTTTTT+TGG + chr4_1:38888886-38888905 Msa0546690:intron 25.0%
! TGCATAATACAGACATAAAA+GGG - chr4_1:38888966-38888985 None:intergenic 25.0%
!!! CTATCATTTTATTTTATGCG+GGG + chr4_1:38888986-38889005 Msa0546690:intron 25.0%
!!! TATCATTTTATTTTATGCGG+GGG + chr4_1:38888987-38889006 Msa0546690:intron 25.0%
! CTATTGTTTGTTTACAGATT+TGG + chr4_1:38889179-38889198 Msa0546690:intron 25.0%
! TCAATATCCAAATGATTAGT+TGG - chr4_1:38890041-38890060 None:intergenic 25.0%
! TCAGGTTTATCTAATAAATC+AGG + chr4_1:38890320-38890339 Msa0546690:three_prime_UTR 25.0%
! GTGCTTATTATTTAATTGCT+TGG + chr4_1:38890538-38890557 Msa0546690:three_prime_UTR 25.0%
! GATAGAATAAGCATCATTTA+AGG - chr4_1:38890606-38890625 None:intergenic 25.0%
AATTCAAACACAAACCCTAA+GGG - chr4_1:38886395-38886414 None:intergenic 30.0%
AAATTCAAACACAAACCCTA+AGG - chr4_1:38886396-38886415 None:intergenic 30.0%
TTGTGTTTGAATTTCGAAGA+TGG + chr4_1:38886402-38886421 Msa0546690:exon 30.0%
! ATCCTCTTTCTAGAGATATT+CGG + chr4_1:38886593-38886612 Msa0546690:CDS 30.0%
TTGATATATGAATGAATCGG+TGG + chr4_1:38886928-38886947 Msa0546690:intron 30.0%
!!! AAGCTTAAGTTTTTTGCTAC+CGG + chr4_1:38886985-38887004 Msa0546690:intron 30.0%
TAATGGTTACATTCACAACA+TGG - chr4_1:38887300-38887319 None:intergenic 30.0%
TACCTCCAAAAAATTGGAAT+AGG - chr4_1:38887327-38887346 None:intergenic 30.0%
!!! TATTCCAATTTTTTGGAGGT+AGG + chr4_1:38887326-38887345 Msa0546690:intron 30.0%
ATTGCAGATAGAGTCTTTAT+CGG + chr4_1:38887443-38887462 Msa0546690:intron 30.0%
!!! TCTTTATCGGTTACTTTTCA+TGG + chr4_1:38887456-38887475 Msa0546690:CDS 30.0%
!! TCTCATTGTTGATTCTGAAT+TGG + chr4_1:38887485-38887504 Msa0546690:CDS 30.0%
TTACTTGGATTAAATGGTTG+TGG + chr4_1:38887656-38887675 Msa0546690:CDS 30.0%
TAAATTTGGTTACAGGATGA+TGG + chr4_1:38887929-38887948 Msa0546690:intron 30.0%
ACGTTCATAAATACGTTCAA+GGG - chr4_1:38887966-38887985 None:intergenic 30.0%
AACGTTCATAAATACGTTCA+AGG - chr4_1:38887967-38887986 None:intergenic 30.0%
TGAACGTATTTATGAACGTT+TGG + chr4_1:38887967-38887986 Msa0546690:CDS 30.0%
AAGTTACCAAGATGATTAGT+TGG - chr4_1:38888166-38888185 None:intergenic 30.0%
GCATAATACAGACATAAAAG+GGG - chr4_1:38888965-38888984 None:intergenic 30.0%
GTGCATAATACAGACATAAA+AGG - chr4_1:38888967-38888986 None:intergenic 30.0%
! GAAGAAGTTTGAACGTATTT+TGG + chr4_1:38889230-38889249 Msa0546690:CDS 30.0%
AGCAATATATTCCTTCATGT+TGG - chr4_1:38889427-38889446 None:intergenic 30.0%
AAGGAATATATTGCTCGATT+TGG + chr4_1:38889432-38889451 Msa0546690:CDS 30.0%
GGAAAAAACACTAGCAAAAA+TGG + chr4_1:38889494-38889513 Msa0546690:CDS 30.0%
CAAAACTTACTTCAGAAAAC+TGG - chr4_1:38889608-38889627 None:intergenic 30.0%
!!! CAGTTTTCTGAAGTAAGTTT+TGG + chr4_1:38889606-38889625 Msa0546690:CDS 30.0%
! TTTTTGTAGATGAGATTCTC+AGG - chr4_1:38889639-38889658 None:intergenic 30.0%
!! ATCTACAAAAACATCGACTT+TGG + chr4_1:38889648-38889667 Msa0546690:CDS 30.0%
!! TCTACAAAAACATCGACTTT+GGG + chr4_1:38889649-38889668 Msa0546690:CDS 30.0%
TTCACTCATCATAAACTGAA+GGG - chr4_1:38889853-38889872 None:intergenic 30.0%
ATTCACTCATCATAAACTGA+AGG - chr4_1:38889854-38889873 None:intergenic 30.0%
CTATGAAGAATTTGTCTGAT+GGG + chr4_1:38889949-38889968 Msa0546690:CDS 30.0%
GAAGTTCTTAGCTAGTATTA+TGG - chr4_1:38890368-38890387 None:intergenic 30.0%
ATCACCACCATTGAAAAAAA+TGG - chr4_1:38890419-38890438 None:intergenic 30.0%
!! GAAGTGCTATCATTTTTGTT+TGG + chr4_1:38890462-38890481 Msa0546690:three_prime_UTR 30.0%
TTGTTTGGATTGAGATAACT+TGG + chr4_1:38890477-38890496 Msa0546690:three_prime_UTR 30.0%
! CATCATTTAAGGTTCAGTTT+AGG - chr4_1:38890595-38890614 None:intergenic 30.0%
GTGATATGAAGATTTCGGAT+CGG + chr4_1:38886545-38886564 Msa0546690:CDS 35.0%
! TCCTCTTTCTAGAGATATTC+GGG + chr4_1:38886594-38886613 Msa0546690:CDS 35.0%
! TTTCTAGAGATATTCGGGTT+AGG + chr4_1:38886599-38886618 Msa0546690:intron 35.0%
AAATTGGAATAGGCGAGTAA+TGG - chr4_1:38887317-38887336 None:intergenic 35.0%
!!! ACTCGCCTATTCCAATTTTT+TGG + chr4_1:38887319-38887338 Msa0546690:intron 35.0%
ACAACCTACCTCCAAAAAAT+TGG - chr4_1:38887333-38887352 None:intergenic 35.0%
CATGCAGTAAACGACAATTA+TGG + chr4_1:38887355-38887374 Msa0546690:intron 35.0%
TCAAAGCAAATCGCAATCAT+TGG - chr4_1:38887615-38887634 None:intergenic 35.0%
! TGATTTTCTTCCAGACGTTA+TGG + chr4_1:38887632-38887651 Msa0546690:intron 35.0%
TCATAACCTGTGATGAAGAA+AGG + chr4_1:38887795-38887814 Msa0546690:CDS 35.0%
TGATGAAGAAAGGTTGCAAT+TGG + chr4_1:38887805-38887824 Msa0546690:CDS 35.0%
! AAGAAGACTCGTGATTTTTC+TGG + chr4_1:38888070-38888089 Msa0546690:CDS 35.0%
GATGAACCAACTAATCATCT+TGG + chr4_1:38888157-38888176 Msa0546690:CDS 35.0%
! TCTTGGTAACTTATCTTACC+TGG + chr4_1:38888174-38888193 Msa0546690:intron 35.0%
!!! ATCACCTTGTCCTGTTTTTT+GGG + chr4_1:38888230-38888249 Msa0546690:intron 35.0%
!! TTTTGGGCACACATTAGTTT+TGG + chr4_1:38888246-38888265 Msa0546690:intron 35.0%
! GCTTGTCATGTTTATGCTTT+TGG + chr4_1:38888358-38888377 Msa0546690:intron 35.0%
TACATGCAAATGATTGTGTC+TGG - chr4_1:38888764-38888783 None:intergenic 35.0%
TGATGCAATATTTCCATGAC+TGG + chr4_1:38889029-38889048 Msa0546690:intron 35.0%
! CAATATTTCCATGACTGGTT+GGG + chr4_1:38889034-38889053 Msa0546690:intron 35.0%
! AATATTTCCATGACTGGTTG+GGG + chr4_1:38889035-38889054 Msa0546690:intron 35.0%
TATTTGTGCAGACACCATTA+AGG - chr4_1:38889281-38889300 None:intergenic 35.0%
AAGAAGCTGAAGATTTACAC+TGG + chr4_1:38889318-38889337 Msa0546690:CDS 35.0%
!! TATATTGCTCGATTTGGTCA+TGG + chr4_1:38889438-38889457 Msa0546690:CDS 35.0%
!! CTACAAAAACATCGACTTTG+GGG + chr4_1:38889650-38889669 Msa0546690:CDS 35.0%
GATATTGAGCAATTCGAAGA+TGG - chr4_1:38889791-38889810 None:intergenic 35.0%
TTTATGATGAGTGAATACCC+TGG + chr4_1:38889858-38889877 Msa0546690:CDS 35.0%
AAATGAGGAAGAGAAGATGA+GGG + chr4_1:38889881-38889900 Msa0546690:CDS 35.0%
CCATCAGACAAATTCTTCAT+AGG - chr4_1:38889951-38889970 None:intergenic 35.0%
CCTATGAAGAATTTGTCTGA+TGG + chr4_1:38889948-38889967 Msa0546690:CDS 35.0%
! TATTGAGACAATTGACTCCT+TGG + chr4_1:38890055-38890074 Msa0546690:CDS 35.0%
GATTAATGAGCCTAAAGTCA+TGG - chr4_1:38890124-38890143 None:intergenic 35.0%
TGACTTTAGGCTCATTAATC+AGG + chr4_1:38890124-38890143 Msa0546690:CDS 35.0%
TGATCAGAAAGTGACCAAAT+GGG + chr4_1:38890172-38890191 Msa0546690:CDS 35.0%
AGCTGTTAGTGATGAGATAT+AGG + chr4_1:38890254-38890273 Msa0546690:three_prime_UTR 35.0%
GCTGTTAGTGATGAGATATA+GGG + chr4_1:38890255-38890274 Msa0546690:three_prime_UTR 35.0%
AGATAAACCTGATGTAAGCA+AGG - chr4_1:38890312-38890331 None:intergenic 35.0%
! AATCAGGTTTGCTTCGATAT+TGG + chr4_1:38890336-38890355 Msa0546690:three_prime_UTR 35.0%
CTTCAGTACAACCAATATCT+TGG + chr4_1:38890384-38890403 Msa0546690:three_prime_UTR 35.0%
AGTACAACCAATATCTTGGT+GGG + chr4_1:38890388-38890407 Msa0546690:three_prime_UTR 35.0%
ACCACCATTGAAAAAAATGG+AGG - chr4_1:38890416-38890435 None:intergenic 35.0%
TTTGGATTGAGATAACTTGG+TGG + chr4_1:38890480-38890499 Msa0546690:three_prime_UTR 35.0%
ATGAGTGGTGAAGTTTGGTA+TGG - chr4_1:38886261-38886280 None:intergenic 40.0%
TGTGGTTTGAAAGAGGAAGA+TGG - chr4_1:38886287-38886306 None:intergenic 40.0%
TTAGGGTTGTGGTTTGAAAG+AGG - chr4_1:38886294-38886313 None:intergenic 40.0%
AAGATAGATCTGGCCTAAGT+CGG - chr4_1:38886354-38886373 None:intergenic 40.0%
AGTGAGAGAGAAGATAGATC+TGG - chr4_1:38886364-38886383 None:intergenic 40.0%
TTCTTCCAAAGCAGCTGATA+AGG + chr4_1:38886507-38886526 Msa0546690:CDS 40.0%
! ACCCGAATATCTCTAGAAAG+AGG - chr4_1:38886598-38886617 None:intergenic 40.0%
!! ATTTCCACATTGGTTTACCG+CGG + chr4_1:38887074-38887093 Msa0546690:intron 40.0%
TTTCTTCTTGAGATGAACCG+CGG - chr4_1:38887094-38887113 None:intergenic 40.0%
!!! CGCCTATTCCAATTTTTTGG+AGG + chr4_1:38887322-38887341 Msa0546690:intron 40.0%
CAATCATTGGTAAGAGTTCC+AGG - chr4_1:38887602-38887621 None:intergenic 40.0%
TCCAGACGTTATGGTTTACT+TGG + chr4_1:38887641-38887660 Msa0546690:intron 40.0%
TCCAAGTAAACCATAACGTC+TGG - chr4_1:38887645-38887664 None:intergenic 40.0%
CATTCCTATTGCAGTAAGCA+GGG - chr4_1:38887690-38887709 None:intergenic 40.0%
TGCTTACTGCAATAGGAATG+CGG + chr4_1:38887690-38887709 Msa0546690:CDS 40.0%
ATATCCATGTGCTCAGGAAT+CGG - chr4_1:38887722-38887741 None:intergenic 40.0%
TGATGAATATCCATGTGCTC+AGG - chr4_1:38887728-38887747 None:intergenic 40.0%
TGGATATTCATCACCTTAGC+AGG + chr4_1:38887735-38887754 Msa0546690:CDS 40.0%
GGATATTCATCACCTTAGCA+GGG + chr4_1:38887736-38887755 Msa0546690:CDS 40.0%
TTGCAACCTTTCTTCATCAC+AGG - chr4_1:38887804-38887823 None:intergenic 40.0%
GGAGAAAGAAGCTGAAACTT+TGG + chr4_1:38887826-38887845 Msa0546690:CDS 40.0%
! ATTTGGTTACAGGATGATGG+AGG + chr4_1:38887932-38887951 Msa0546690:intron 40.0%
GCTGAAATCTTAGATGGTCT+TGG + chr4_1:38888025-38888044 Msa0546690:CDS 40.0%
! AAGACTCGTGATTTTTCTGG+AGG + chr4_1:38888073-38888092 Msa0546690:CDS 40.0%
TCATCGAGTAGAAGAATGGT+GGG - chr4_1:38888142-38888161 None:intergenic 40.0%
TTCATCGAGTAGAAGAATGG+TGG - chr4_1:38888143-38888162 None:intergenic 40.0%
TGGTTCATCGAGTAGAAGAA+TGG - chr4_1:38888146-38888165 None:intergenic 40.0%
AAACAAGAGGAGTTCTTTCC+AGG - chr4_1:38888195-38888214 None:intergenic 40.0%
!!! CATCACCTTGTCCTGTTTTT+TGG + chr4_1:38888229-38888248 Msa0546690:intron 40.0%
CTAATGTGTGCCCAAAAAAC+AGG - chr4_1:38888243-38888262 None:intergenic 40.0%
GCAATATTTCCATGACTGGT+TGG + chr4_1:38889033-38889052 Msa0546690:intron 40.0%
CATTCCCAGGATTTCCTTAA+TGG + chr4_1:38889264-38889283 Msa0546690:CDS 40.0%
GACACCATTAAGGAAATCCT+GGG - chr4_1:38889271-38889290 None:intergenic 40.0%
AGACACCATTAAGGAAATCC+TGG - chr4_1:38889272-38889291 None:intergenic 40.0%
TCAGCTTCTTACTTTGCATG+TGG - chr4_1:38889308-38889327 None:intergenic 40.0%
GATCAATATGTTCAGACTCG+TGG + chr4_1:38889348-38889367 Msa0546690:CDS 40.0%
ACACTAGCAAAAATGGAACG+AGG + chr4_1:38889501-38889520 Msa0546690:CDS 40.0%
ACTTCAGAAAACTGGAGAAC+AGG - chr4_1:38889600-38889619 None:intergenic 40.0%
!! GGTGATTTGTTTCCTAGTGA+TGG + chr4_1:38889747-38889766 Msa0546690:CDS 40.0%
TTTGTTTCCTAGTGATGGCA+TGG + chr4_1:38889752-38889771 Msa0546690:CDS 40.0%
TGCTCAATATCATCAGCACT+TGG + chr4_1:38889800-38889819 Msa0546690:CDS 40.0%
TCTTCTCTTCCTCATTTCCA+GGG - chr4_1:38889878-38889897 None:intergenic 40.0%
ATCTTCTCTTCCTCATTTCC+AGG - chr4_1:38889879-38889898 None:intergenic 40.0%
GAAATGAGGAAGAGAAGATG+AGG + chr4_1:38889880-38889899 Msa0546690:CDS 40.0%
ATTCTTCATAGGCATCACCT+GGG - chr4_1:38889940-38889959 None:intergenic 40.0%
AATTCTTCATAGGCATCACC+TGG - chr4_1:38889941-38889960 None:intergenic 40.0%
AGAGTCGAGTAATCTTTGCA+TGG + chr4_1:38889976-38889995 Msa0546690:CDS 40.0%
TCATCCAGAAGTAGCAATTG+AGG - chr4_1:38890017-38890036 None:intergenic 40.0%
GGATGAGCCAACTAATCATT+TGG + chr4_1:38890031-38890050 Msa0546690:CDS 40.0%
! TGCTTGTTAGCCATGACTTT+AGG + chr4_1:38890111-38890130 Msa0546690:CDS 40.0%
CTTTAGGCTCATTAATCAGG+TGG + chr4_1:38890127-38890146 Msa0546690:CDS 40.0%
CTGATCAGAAAGTGACCAAA+TGG + chr4_1:38890171-38890190 Msa0546690:CDS 40.0%
CAGTACAACCAATATCTTGG+TGG + chr4_1:38890387-38890406 Msa0546690:three_prime_UTR 40.0%
!!! GGTGCCTCCATTTTTTTCAA+TGG + chr4_1:38890409-38890428 Msa0546690:three_prime_UTR 40.0%
!!! GCCTCCATTTTTTTCAATGG+TGG + chr4_1:38890412-38890431 Msa0546690:three_prime_UTR 40.0%
GTGGTGATTGTTGAGTTCAA+TGG + chr4_1:38890431-38890450 Msa0546690:three_prime_UTR 40.0%
TTGACCAGCAAGATGGATAA+TGG + chr4_1:38890564-38890583 Msa0546690:three_prime_UTR 40.0%
TGACCAGCAAGATGGATAAT+GGG + chr4_1:38890565-38890584 Msa0546690:three_prime_UTR 40.0%
GGAGTATGAGTGGTGAAGTT+TGG - chr4_1:38886266-38886285 None:intergenic 45.0%
AGAGGAAGATGGAGTATGAG+TGG - chr4_1:38886276-38886295 None:intergenic 45.0%
AAGGGTTGAGATTAGGGTTG+TGG - chr4_1:38886305-38886324 None:intergenic 45.0%
CGAACGAAGGGTTGAGATTA+GGG - chr4_1:38886311-38886330 None:intergenic 45.0%
CCTAATCTCAACCCTTCGTT+CGG + chr4_1:38886309-38886328 Msa0546690:five_prime_UTR 45.0%
AAAAGATGGAGCCGAACGAA+GGG - chr4_1:38886323-38886342 None:intergenic 45.0%
! GTTCGGCTCCATCTTTTCAT+CGG + chr4_1:38886326-38886345 Msa0546690:five_prime_UTR 45.0%
! AGTCGGTTCCGATGAAAAGA+TGG - chr4_1:38886337-38886356 None:intergenic 45.0%
!! CTTTTCATCGGAACCGACTT+AGG + chr4_1:38886338-38886357 Msa0546690:five_prime_UTR 45.0%
! TCTCACTCTTTTCCTCCCTT+AGG + chr4_1:38886377-38886396 Msa0546690:five_prime_UTR 45.0%
! CTCACTCTTTTCCTCCCTTA+GGG + chr4_1:38886378-38886397 Msa0546690:five_prime_UTR 45.0%
TCAAACACAAACCCTAAGGG+AGG - chr4_1:38886392-38886411 None:intergenic 45.0%
AGCTTTCTTCTTGCTAGCGT+CGG - chr4_1:38886431-38886450 None:intergenic 45.0%
ATTTCGGATCGGACTTGTAC+TGG + chr4_1:38886556-38886575 Msa0546690:CDS 45.0%
AGTTTAGCGAGTCTTGCTTC+TGG + chr4_1:38887165-38887184 Msa0546690:intron 45.0%
!! TGCTTCTGGCTTTATGTTGC+TGG + chr4_1:38887179-38887198 Msa0546690:intron 45.0%
!! CTGGCTTTATGTTGCTGGTT+TGG + chr4_1:38887184-38887203 Msa0546690:intron 45.0%
TCTACCCTGCTTACTGCAAT+AGG + chr4_1:38887683-38887702 Msa0546690:CDS 45.0%
GCATTCCTATTGCAGTAAGC+AGG - chr4_1:38887691-38887710 None:intergenic 45.0%
GCTTACTGCAATAGGAATGC+GGG + chr4_1:38887691-38887710 Msa0546690:CDS 45.0%
AGGCTTCAATTTCCCTGCTA+AGG - chr4_1:38887751-38887770 None:intergenic 45.0%
TTCCAATGCAGACATGTCAG+AGG - chr4_1:38887771-38887790 None:intergenic 45.0%
! TTCTGCGGTTGATGCATCTA+GGG - chr4_1:38887996-38888015 None:intergenic 45.0%
! TTTCTGCGGTTGATGCATCT+AGG - chr4_1:38887997-38888016 None:intergenic 45.0%
! CGTGCTGCTGAAATCTTAGA+TGG + chr4_1:38888019-38888038 Msa0546690:CDS 45.0%
!!! TGGTTTTGACAAGCAGATGC+AGG + chr4_1:38888045-38888064 Msa0546690:CDS 45.0%
!! CTCGTGATTTTTCTGGAGGA+TGG + chr4_1:38888077-38888096 Msa0546690:CDS 45.0%
CATCGAGTAGAAGAATGGTG+GGG - chr4_1:38888141-38888160 None:intergenic 45.0%
TGTGCCCAAAAAACAGGACA+AGG - chr4_1:38888237-38888256 None:intergenic 45.0%
CAGATTTGGAAGCTTGTGTG+TGG + chr4_1:38889193-38889212 Msa0546690:intron 45.0%
GGTTGTGATATCACATTCCC+AGG + chr4_1:38889251-38889270 Msa0546690:CDS 45.0%
ACCAGATGAAGCAGTACAAG+TGG + chr4_1:38889385-38889404 Msa0546690:CDS 45.0%
CCAGATGAAGCAGTACAAGT+GGG + chr4_1:38889386-38889405 Msa0546690:CDS 45.0%
!!! TTTTTTCCTTGCTCTGTGCC+TGG - chr4_1:38889482-38889501 None:intergenic 45.0%
CTAGCAAAAATGGAACGAGG+TGG + chr4_1:38889504-38889523 Msa0546690:CDS 45.0%
AGTATTCCGCTTCACTGATG+TGG + chr4_1:38889560-38889579 Msa0546690:CDS 45.0%
GTATTCCGCTTCACTGATGT+GGG + chr4_1:38889561-38889580 Msa0546690:CDS 45.0%
! GTTTTCCCACATCAGTGAAG+CGG - chr4_1:38889569-38889588 None:intergenic 45.0%
AGAAAACTGGAGAACAGGAG+GGG - chr4_1:38889595-38889614 None:intergenic 45.0%
CAGAAAACTGGAGAACAGGA+GGG - chr4_1:38889596-38889615 None:intergenic 45.0%
TCAGAAAACTGGAGAACAGG+AGG - chr4_1:38889597-38889616 None:intergenic 45.0%
! TTGGGGTTGACTTAGACTCT+AGG + chr4_1:38889667-38889686 Msa0546690:CDS 45.0%
!! CTTAGACTCTAGGATTGCTC+TGG + chr4_1:38889677-38889696 Msa0546690:CDS 45.0%
ATTGCTCTGGTTGGACCAAA+TGG + chr4_1:38889690-38889709 Msa0546690:CDS 45.0%
GTACTCTTTCCAGCACCATT+TGG - chr4_1:38889708-38889727 None:intergenic 45.0%
ACACTGCTGAAGCTAATGAC+AGG + chr4_1:38889726-38889745 Msa0546690:CDS 45.0%
TCAGCACTTGGCTGAGAAAT+TGG + chr4_1:38889812-38889831 Msa0546690:CDS 45.0%
CTTGGCTGAGAAATTGGACT+TGG + chr4_1:38889818-38889837 Msa0546690:CDS 45.0%
CTCATCATAAACTGAAGGGC+AGG - chr4_1:38889849-38889868 None:intergenic 45.0%
GAGTGAATACCCTGGAAATG+AGG + chr4_1:38889866-38889885 Msa0546690:CDS 45.0%
AGAAGATGAGGGCTGCTATT+GGG + chr4_1:38889892-38889911 Msa0546690:CDS 45.0%
! ACAGCCTCAATTGCTACTTC+TGG + chr4_1:38890010-38890029 Msa0546690:CDS 45.0%
ATCCCACTCATTCAATGCCT+CGG - chr4_1:38890081-38890100 None:intergenic 45.0%
TCAGAAAGTGACCAAATGGG+AGG + chr4_1:38890175-38890194 Msa0546690:CDS 45.0%
CAGAAAGTGACCAAATGGGA+GGG + chr4_1:38890176-38890195 Msa0546690:CDS 45.0%
CATAATGTCACCCTCCCATT+TGG - chr4_1:38890189-38890208 None:intergenic 45.0%
CAAATGGGAGGGTGACATTA+TGG + chr4_1:38890187-38890206 Msa0546690:CDS 45.0%
!! CAAGAAGCATCTGAAGACGA+AGG + chr4_1:38890214-38890233 Msa0546690:CDS 45.0%
CAAGGCCATAAGTCTCCAAA+GGG - chr4_1:38890294-38890313 None:intergenic 45.0%
! CTTATGGCCTTGCTTACATC+AGG + chr4_1:38890302-38890321 Msa0546690:three_prime_UTR 45.0%
GTTGAGTTCAATGGCAGAGT+CGG + chr4_1:38890440-38890459 Msa0546690:three_prime_UTR 45.0%
! TTGGTTCTTGACCAGCAAGA+TGG + chr4_1:38890557-38890576 Msa0546690:three_prime_UTR 45.0%
TGTCCCATTATCCATCTTGC+TGG - chr4_1:38890571-38890590 None:intergenic 45.0%
CCGAACGAAGGGTTGAGATT+AGG - chr4_1:38886312-38886331 None:intergenic 50.0%
GAAAAGATGGAGCCGAACGA+AGG - chr4_1:38886324-38886343 None:intergenic 50.0%
!! AGCTGCTTTGGAAGAAGCAG+CGG - chr4_1:38886503-38886522 None:intergenic 50.0%
AGCGACCTTATCAGCTGCTT+TGG - chr4_1:38886515-38886534 None:intergenic 50.0%
TGAACCGCGGTAAACCAATG+TGG - chr4_1:38887081-38887100 None:intergenic 50.0%
ATGCTACGTACCCATCAGCT+CGG - chr4_1:38887267-38887286 None:intergenic 50.0%
! AGCTGATGGGTACGTAGCAT+GGG + chr4_1:38887267-38887286 Msa0546690:intron 50.0%
ACTTCCGATTCCTGAGCACA+TGG + chr4_1:38887715-38887734 Msa0546690:CDS 50.0%
AGCCTCTGACATGTCTGCAT+TGG + chr4_1:38887766-38887785 Msa0546690:CDS 50.0%
AGCTGAAACTTTGGCTGCTC+AGG + chr4_1:38887835-38887854 Msa0546690:CDS 50.0%
!! TGGTTACAGGATGATGGAGG+TGG + chr4_1:38887935-38887954 Msa0546690:intron 50.0%
! TTCAGCAGCACGCTTTTCTG+CGG - chr4_1:38888011-38888030 None:intergenic 50.0%
GAAACATCCCCAACCAGTCA+TGG - chr4_1:38889045-38889064 None:intergenic 50.0%
TGTTCAGACTCGTGGTGACT+TGG + chr4_1:38889356-38889375 Msa0546690:CDS 50.0%
CCCACTTGTACTGCTTCATC+TGG - chr4_1:38889389-38889408 None:intergenic 50.0%
GAAGCAGTACAAGTGGGAAC+AGG + chr4_1:38889392-38889411 Msa0546690:CDS 50.0%
GGAGCAGATTGCCAACATGA+AGG + chr4_1:38889413-38889432 Msa0546690:CDS 50.0%
TTCAGCAAAACTAGCTCGCC+AGG + chr4_1:38889461-38889480 Msa0546690:CDS 50.0%
AAAACTGGAGAACAGGAGGG+GGG - chr4_1:38889593-38889612 None:intergenic 50.0%
GAAAACTGGAGAACAGGAGG+GGG - chr4_1:38889594-38889613 None:intergenic 50.0%
!! GACTCTAGGATTGCTCTGGT+TGG + chr4_1:38889681-38889700 Msa0546690:CDS 50.0%
GAGAAGATGAGGGCTGCTAT+TGG + chr4_1:38889891-38889910 Msa0546690:CDS 50.0%
ATTGGGAGGTTTGGACTGAC+AGG + chr4_1:38889909-38889928 Msa0546690:CDS 50.0%
!! GAGGCATTGAATGAGTGGGA+TGG + chr4_1:38890080-38890099 Msa0546690:CDS 50.0%
GCATTGAATGAGTGGGATGG+CGG + chr4_1:38890083-38890102 Msa0546690:CDS 50.0%
ATCAGGTGGCGCATGAGATA+TGG + chr4_1:38890141-38890160 Msa0546690:CDS 50.0%
TCAGGTGGCGCATGAGATAT+GGG + chr4_1:38890142-38890161 Msa0546690:CDS 50.0%
! GAAGCATCTGAAGACGAAGG+CGG + chr4_1:38890217-38890236 Msa0546690:CDS 50.0%
AAGCATCTGAAGACGAAGGC+GGG + chr4_1:38890218-38890237 Msa0546690:CDS 50.0%
GGATAGAAACCACCACCCTT+TGG + chr4_1:38890276-38890295 Msa0546690:three_prime_UTR 50.0%
CATAAGTCTCCAAAGGGTGG+TGG - chr4_1:38890288-38890307 None:intergenic 50.0%
! CACCACCCTTTGGAGACTTA+TGG + chr4_1:38890286-38890305 Msa0546690:three_prime_UTR 50.0%
GCAAGGCCATAAGTCTCCAA+AGG - chr4_1:38890295-38890314 None:intergenic 50.0%
CTTACCACCACGTTTAGCGG+CGG - chr4_1:38886473-38886492 None:intergenic 55.0%
GGCCTTACCACCACGTTTAG+CGG - chr4_1:38886476-38886495 None:intergenic 55.0%
TTTGGAAGAAGCAGCGGCTG+CGG - chr4_1:38886497-38886516 None:intergenic 55.0%
! GAGCTGATGGGTACGTAGCA+TGG + chr4_1:38887266-38887285 Msa0546690:intron 55.0%
! ACGAGGTGGACTCACAGAGA+AGG + chr4_1:38889518-38889537 Msa0546690:CDS 55.0%
CTGGTTGGACCAAATGGTGC+TGG + chr4_1:38889696-38889715 Msa0546690:CDS 55.0%
CGCTTGACCATGCCATCACT+AGG - chr4_1:38889762-38889781 None:intergenic 55.0%
AGATGAGGGCTGCTATTGGG+AGG + chr4_1:38889895-38889914 Msa0546690:CDS 55.0%
AGGGCTGCTATTGGGAGGTT+TGG + chr4_1:38889900-38889919 Msa0546690:CDS 55.0%
TGGACTGACAGGTAAAGCCC+AGG + chr4_1:38889920-38889939 Msa0546690:CDS 55.0%
! GACAATTGACTCCTTGGCCG+AGG + chr4_1:38890061-38890080 Msa0546690:CDS 55.0%
CTCATTCAATGCCTCGGCCA+AGG - chr4_1:38890075-38890094 None:intergenic 55.0%
!! TGGCCGAGGCATTGAATGAG+TGG + chr4_1:38890075-38890094 Msa0546690:CDS 55.0%
!! GGCCGAGGCATTGAATGAGT+GGG + chr4_1:38890076-38890095 Msa0546690:CDS 55.0%
AGCATCTGAAGACGAAGGCG+GGG + chr4_1:38890219-38890238 Msa0546690:CDS 55.0%
GAAGACGAAGGCGGGGATAT+TGG + chr4_1:38890226-38890245 Msa0546690:CDS 55.0%
GGCCATAAGTCTCCAAAGGG+TGG - chr4_1:38890291-38890310 None:intergenic 55.0%
!! GGAGGCACCCACCAAGATAT+TGG - chr4_1:38890398-38890417 None:intergenic 55.0%
CGCCGCTAAACGTGGTGGTA+AGG + chr4_1:38886471-38886490 Msa0546690:CDS 60.0%
TGAGCGTCTAGCCGAGCTGA+TGG + chr4_1:38887253-38887272 Msa0546690:intron 60.0%
GAGCGTCTAGCCGAGCTGAT+GGG + chr4_1:38887254-38887273 Msa0546690:intron 60.0%
! GAATGAGTGGGATGGCGGCA+TGG + chr4_1:38890088-38890107 Msa0546690:CDS 60.0%
ACCACCACGTTTAGCGGCGG+CGG - chr4_1:38886470-38886489 None:intergenic 65.0%
AGCTCGCCAGGCACAGAGCA+AGG + chr4_1:38889473-38889492 Msa0546690:CDS 65.0%
ACCACGTTTAGCGGCGGCGG+CGG - chr4_1:38886467-38886486 None:intergenic 70.0%
GCCGCCGCCGCTAAACGTGG+TGG + chr4_1:38886466-38886485 Msa0546690:CDS 75.0%
! GCCGCCGCCGCCGCTAAACG+TGG + chr4_1:38886463-38886482 Msa0546690:CDS 80.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 38886235 38890723 38886235 ID=Msa0546690;Name=Msa0546690
chr4_1 mRNA 38886235 38890723 38886235 ID=Msa0546690-mRNA-1;Parent=Msa0546690;Name=Msa0546690-mRNA-1;_AED=0.03;_eAED=0.03;_QI=186|1|1|1|1|1|5|470|590
chr4_1 exon 38886235 38886615 38886235 ID=Msa0546690-mRNA-1:exon:9926;Parent=Msa0546690-mRNA-1
chr4_1 exon 38887450 38887523 38887450 ID=Msa0546690-mRNA-1:exon:9927;Parent=Msa0546690-mRNA-1
chr4_1 exon 38887646 38887856 38887646 ID=Msa0546690-mRNA-1:exon:9928;Parent=Msa0546690-mRNA-1
chr4_1 exon 38887944 38888178 38887944 ID=Msa0546690-mRNA-1:exon:9929;Parent=Msa0546690-mRNA-1
chr4_1 exon 38889196 38890723 38889196 ID=Msa0546690-mRNA-1:exon:9930;Parent=Msa0546690-mRNA-1
chr4_1 five_prime_UTR 38886235 38886420 38886235 ID=Msa0546690-mRNA-1:five_prime_utr;Parent=Msa0546690-mRNA-1
chr4_1 CDS 38886421 38886615 38886421 ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1 CDS 38887450 38887523 38887450 ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1 CDS 38887646 38887856 38887646 ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1 CDS 38887944 38888178 38887944 ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1 CDS 38889196 38890253 38889196 ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1 three_prime_UTR 38890254 38890723 38890254 ID=Msa0546690-mRNA-1:three_prime_utr;Parent=Msa0546690-mRNA-1
Gene Sequence

>Msa0546690

ATGGTTTCCGACGCTAGCAAGAAGAAAGCTGCTCAGAAGAAAGCCGCCGCCGCCGCTAAACGTGGTGGTAAGGCCGCAGCCGCTGCTTCTTCCAAAGCAGCTGATAAGGTCGCTGATAAAATCAGTGATATGAAGATTTCGGATCGGACTTGTACTGGTGTTCTTTGTTCTCATCCTCTTTCTAGAGATATTCGGATAGAGTCTTTATCGGTTACTTTTCATGGACATGATCTCATTGTTGATTCTGAATTGGAGTTAAATTATGGAAGACGTTATGGTTTACTTGGATTAAATGGTTGTGGAAAGTCTACCCTGCTTACTGCAATAGGAATGCGGGAACTTCCGATTCCTGAGCACATGGATATTCATCACCTTAGCAGGGAAATTGAAGCCTCTGACATGTCTGCATTGGAAGCTGTCATAACCTGTGATGAAGAAAGGTTGCAATTGGAGAAAGAAGCTGAAACTTTGGCTGCTCAGGATGATGGAGGTGGTGAAGCCCTTGAACGTATTTATGAACGTTTGGACGCCCTAGATGCATCAACCGCAGAAAAGCGTGCTGCTGAAATCTTAGATGGTCTTGGTTTTGACAAGCAGATGCAGGCAAAGAAGACTCGTGATTTTTCTGGAGGATGGAGAATGAGAATTTCATTAGCACGAGCTTTATTTATGAACCCCACCATTCTTCTACTCGATGAACCAACTAATCATCTTGATTTGGAAGCTTGTGTGTGGCTCGAAGAGAGTTTGAAGAAGTTTGAACGTATTTTGGTTGTGATATCACATTCCCAGGATTTCCTTAATGGTGTCTGCACAAATATTATCCACATGCAAAGTAAGAAGCTGAAGATTTACACTGGCAACTATGATCAATATGTTCAGACTCGTGGTGACTTGGAAGAGAACCAGATGAAGCAGTACAAGTGGGAACAGGAGCAGATTGCCAACATGAAGGAATATATTGCTCGATTTGGTCATGGTTCAGCAAAACTAGCTCGCCAGGCACAGAGCAAGGAAAAAACACTAGCAAAAATGGAACGAGGTGGACTCACAGAGAAGGTAGTCAGAGATCAAGTTTTAGTATTCCGCTTCACTGATGTGGGAAAACTCCCCCCTCCTGTTCTCCAGTTTTCTGAAGTAAGTTTTGGTTACACTCCTGAGAATCTCATCTACAAAAACATCGACTTTGGGGTTGACTTAGACTCTAGGATTGCTCTGGTTGGACCAAATGGTGCTGGAAAGAGTACACTGCTGAAGCTAATGACAGGTGATTTGTTTCCTAGTGATGGCATGGTCAAGCGTCATAACCATCTTCGAATTGCTCAATATCATCAGCACTTGGCTGAGAAATTGGACTTGGATGTGCCTGCCCTTCAGTTTATGATGAGTGAATACCCTGGAAATGAGGAAGAGAAGATGAGGGCTGCTATTGGGAGGTTTGGACTGACAGGTAAAGCCCAGGTGATGCCTATGAAGAATTTGTCTGATGGGCAAAAGAGTCGAGTAATCTTTGCATGGTTAGCTTATAGACAGCCTCAATTGCTACTTCTGGATGAGCCAACTAATCATTTGGATATTGAGACAATTGACTCCTTGGCCGAGGCATTGAATGAGTGGGATGGCGGCATGGTGCTTGTTAGCCATGACTTTAGGCTCATTAATCAGGTGGCGCATGAGATATGGGTGTGTGCTGATCAGAAAGTGACCAAATGGGAGGGTGACATTATGGATTTCAAGAAGCATCTGAAGACGAAGGCGGGGATATTGGACTGA
Protein sequence

>Msa0546690

MVSDASKKKAAQKKAAAAAKRGGKAAAAASSKAADKVADKISDMKISDRTCTGVLCSHPLSRDIRIESLSVTFHGHDLIVDSELELNYGRRYGLLGLNGCGKSTLLTAIGMRELPIPEHMDIHHLSREIEASDMSALEAVITCDEERLQLEKEAETLAAQDDGGGEALERIYERLDALDASTAEKRAAEILDGLGFDKQMQAKKTRDFSGGWRMRISLARALFMNPTILLLDEPTNHLDLEACVWLEESLKKFERILVVISHSQDFLNGVCTNIIHMQSKKLKIYTGNYDQYVQTRGDLEENQMKQYKWEQEQIANMKEYIARFGHGSAKLARQAQSKEKTLAKMERGGLTEKVVRDQVLVFRFTDVGKLPPPVLQFSEVSFGYTPENLIYKNIDFGVDLDSRIALVGPNGAGKSTLLKLMTGDLFPSDGMVKRHNHLRIAQYHQHLAEKLDLDVPALQFMMSEYPGNEEEKMRAAIGRFGLTGKAQVMPMKNLSDGQKSRVIFAWLAYRQPQLLLLDEPTNHLDIETIDSLAEALNEWDGGMVLVSHDFRLINQVAHEIWVCADQKVTKWEGDIMDFKKHLKTKAGILD*