AlfalfaGEDB | Msa0546690

M. sativa cultivar zhongmu-4 / Msa0546690

KEGG: K06185
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF00005.30

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0546690	XP_013455966.1	99.661	590	2	0	1	590	1	590	0.0	1220

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0546690	sp\|Q9FJH6\|AB1F_ARATH	84.409	558	87	0	33	590	38	595	0.0	1008

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0546690	A0A072UJY1	99.661	590	2	0	1	590	1	590	0.0	1220

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0270200	Msa0546690	0.814573	1.438473e-51	-8.615850e-47
Msa0428210	Msa0546690	0.810799	9.629445e-51	-8.615850e-47
Msa0546690	Msa0639490	0.846879	1.645509e-59	-8.615850e-47
Msa0546690	Msa1403560	0.824951	6.111702e-54	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0546690	MtrunA17_Chr4g0028471	99.661	590	2	0	1	590	1	590	0.0	1220
Msa0546690	MtrunA17_Chr2g0327291	89.322	590	63	0	1	590	1	590	0.0	1082
Msa0546690	MtrunA17_Chr3g0119921	43.382	544	283	6	62	584	173	712	5.98e-156	464
Msa0546690	MtrunA17_Chr7g0220881	40.179	560	290	6	62	582	152	705	6.79e-144	433
Msa0546690	MtrunA17_Chr8g0371361	41.547	556	286	8	62	582	152	703	1.96e-143	432
Msa0546690	MtrunA17_Chr1g0181461	41.667	552	285	9	66	582	2	551	1.59e-141	422
Msa0546690	MtrunA17_Chr3g0129301	29.202	589	386	9	11	574	50	632	4.32e-70	239
Msa0546690	MtrunA17_Chr3g0129301	26.316	247	149	4	64	306	433	650	1.46e-19	92.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0546690	AT5G60790.1	84.409	558	87	0	33	590	38	595	0.0	1008
Msa0546690	AT1G64550.1	44.404	545	277	6	62	584	173	713	4.05e-158	470
Msa0546690	AT3G54540.2	42.602	561	276	9	62	583	161	714	4.54e-152	454
Msa0546690	AT3G54540.1	42.602	561	276	9	62	583	161	714	4.54e-152	454
Msa0546690	AT5G64840.1	31.273	534	345	7	60	574	94	624	6.33e-75	252
Msa0546690	AT5G64840.1	25.311	241	143	3	64	298	425	634	2.37e-17	86.3
Msa0546690	AT5G09930.1	31.047	554	353	8	46	578	69	614	1.43e-73	248
Msa0546690	AT5G09930.1	25.726	241	134	7	64	294	411	616	1.71e-15	80.5
Msa0546690	AT3G28860.1	28.856	201	128	6	81	276	1030	1220	1.76e-11	67.8
Msa0546690	AT1G28010.1	30.342	234	123	8	375	581	1006	1226	8.98e-11	65.9

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Find 205 sgRNAs with CRISPR-Local

Find 314 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGGAGAACAGGAGGG+GGG	0.666914	4_1:-38889590	None:intergenic
AAAAGATGGAGCCGAACGAA+GGG	0.584128	4_1:-38886320	None:intergenic
AAATGAGGAAGAGAAGATGA+GGG	0.639167	4_1:+38889881	Msa0546690:CDS
AAATTCAAACACAAACCCTA+AGG	0.565291	4_1:-38886393	None:intergenic
AACGTTCATAAATACGTTCA+AGG	0.466725	4_1:-38887964	None:intergenic
AAGAAGCTGAAGATTTACAC+TGG	0.638565	4_1:+38889318	Msa0546690:CDS
AAGATAGATCTGGCCTAAGT+CGG	0.568371	4_1:-38886351	None:intergenic
AAGCATCTGAAGACGAAGGC+GGG	0.463027	4_1:+38890218	Msa0546690:CDS
AAGGAATATATTGCTCGATT+TGG	0.324399	4_1:+38889432	Msa0546690:CDS
AAGGGTTGAGATTAGGGTTG+TGG	0.409947	4_1:-38886302	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTCAAACACAAACCCTAA+GGG	-	chr4_1:38886395-38886414	None:intergenic	30.0%
AAATTCAAACACAAACCCTA+AGG	-	chr4_1:38886396-38886415	None:intergenic	30.0%
TTGTGTTTGAATTTCGAAGA+TGG	+	chr4_1:38886402-38886421	Msa0546690:exon	30.0%
TTGATATATGAATGAATCGG+TGG	+	chr4_1:38886928-38886947	Msa0546690:intron	30.0%
TAATGGTTACATTCACAACA+TGG	-	chr4_1:38887300-38887319	None:intergenic	30.0%
TACCTCCAAAAAATTGGAAT+AGG	-	chr4_1:38887327-38887346	None:intergenic	30.0%
ATTGCAGATAGAGTCTTTAT+CGG	+	chr4_1:38887443-38887462	Msa0546690:intron	30.0%
TTACTTGGATTAAATGGTTG+TGG	+	chr4_1:38887656-38887675	Msa0546690:CDS	30.0%
TAAATTTGGTTACAGGATGA+TGG	+	chr4_1:38887929-38887948	Msa0546690:intron	30.0%
ACGTTCATAAATACGTTCAA+GGG	-	chr4_1:38887966-38887985	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_1	gene	38886235	38890723	38886235	ID=Msa0546690;Name=Msa0546690
chr4_1	mRNA	38886235	38890723	38886235	ID=Msa0546690-mRNA-1;Parent=Msa0546690;Name=Msa0546690-mRNA-1;_AED=0.03;_eAED=0.03;_QI=186\|1\|1\|1\|1\|1\|5\|470\|590
chr4_1	exon	38886235	38886615	38886235	ID=Msa0546690-mRNA-1:exon:9926;Parent=Msa0546690-mRNA-1
chr4_1	exon	38887450	38887523	38887450	ID=Msa0546690-mRNA-1:exon:9927;Parent=Msa0546690-mRNA-1
chr4_1	exon	38887646	38887856	38887646	ID=Msa0546690-mRNA-1:exon:9928;Parent=Msa0546690-mRNA-1
chr4_1	exon	38887944	38888178	38887944	ID=Msa0546690-mRNA-1:exon:9929;Parent=Msa0546690-mRNA-1
chr4_1	exon	38889196	38890723	38889196	ID=Msa0546690-mRNA-1:exon:9930;Parent=Msa0546690-mRNA-1
chr4_1	five_prime_UTR	38886235	38886420	38886235	ID=Msa0546690-mRNA-1:five_prime_utr;Parent=Msa0546690-mRNA-1
chr4_1	CDS	38886421	38886615	38886421	ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1	CDS	38887450	38887523	38887450	ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1	CDS	38887646	38887856	38887646	ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1	CDS	38887944	38888178	38887944	ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1	CDS	38889196	38890253	38889196	ID=Msa0546690-mRNA-1:cds;Parent=Msa0546690-mRNA-1
chr4_1	three_prime_UTR	38890254	38890723	38890254	ID=Msa0546690-mRNA-1:three_prime_utr;Parent=Msa0546690-mRNA-1

Gene Sequence

>Msa0546690

ATGGTTTCCGACGCTAGCAAGAAGAAAGCTGCTCAGAAGAAAGCCGCCGCCGCCGCTAAACGTGGTGGTAAGGCCGCAGCCGCTGCTTCTTCCAAAGCAGCTGATAAGGTCGCTGATAAAATCAGTGATATGAAGATTTCGGATCGGACTTGTACTGGTGTTCTTTGTTCTCATCCTCTTTCTAGAGATATTCGGATAGAGTCTTTATCGGTTACTTTTCATGGACATGATCTCATTGTTGATTCTGAATTGGAGTTAAATTATGGAAGACGTTATGGTTTACTTGGATTAAATGGTTGTGGAAAGTCTACCCTGCTTACTGCAATAGGAATGCGGGAACTTCCGATTCCTGAGCACATGGATATTCATCACCTTAGCAGGGAAATTGAAGCCTCTGACATGTCTGCATTGGAAGCTGTCATAACCTGTGATGAAGAAAGGTTGCAATTGGAGAAAGAAGCTGAAACTTTGGCTGCTCAGGATGATGGAGGTGGTGAAGCCCTTGAACGTATTTATGAACGTTTGGACGCCCTAGATGCATCAACCGCAGAAAAGCGTGCTGCTGAAATCTTAGATGGTCTTGGTTTTGACAAGCAGATGCAGGCAAAGAAGACTCGTGATTTTTCTGGAGGATGGAGAATGAGAATTTCATTAGCACGAGCTTTATTTATGAACCCCACCATTCTTCTACTCGATGAACCAACTAATCATCTTGATTTGGAAGCTTGTGTGTGGCTCGAAGAGAGTTTGAAGAAGTTTGAACGTATTTTGGTTGTGATATCACATTCCCAGGATTTCCTTAATGGTGTCTGCACAAATATTATCCACATGCAAAGTAAGAAGCTGAAGATTTACACTGGCAACTATGATCAATATGTTCAGACTCGTGGTGACTTGGAAGAGAACCAGATGAAGCAGTACAAGTGGGAACAGGAGCAGATTGCCAACATGAAGGAATATATTGCTCGATTTGGTCATGGTTCAGCAAAACTAGCTCGCCAGGCACAGAGCAAGGAAAAAACACTAGCAAAAATGGAACGAGGTGGACTCACAGAGAAGGTAGTCAGAGATCAAGTTTTAGTATTCCGCTTCACTGATGTGGGAAAACTCCCCCCTCCTGTTCTCCAGTTTTCTGAAGTAAGTTTTGGTTACACTCCTGAGAATCTCATCTACAAAAACATCGACTTTGGGGTTGACTTAGACTCTAGGATTGCTCTGGTTGGACCAAATGGTGCTGGAAAGAGTACACTGCTGAAGCTAATGACAGGTGATTTGTTTCCTAGTGATGGCATGGTCAAGCGTCATAACCATCTTCGAATTGCTCAATATCATCAGCACTTGGCTGAGAAATTGGACTTGGATGTGCCTGCCCTTCAGTTTATGATGAGTGAATACCCTGGAAATGAGGAAGAGAAGATGAGGGCTGCTATTGGGAGGTTTGGACTGACAGGTAAAGCCCAGGTGATGCCTATGAAGAATTTGTCTGATGGGCAAAAGAGTCGAGTAATCTTTGCATGGTTAGCTTATAGACAGCCTCAATTGCTACTTCTGGATGAGCCAACTAATCATTTGGATATTGAGACAATTGACTCCTTGGCCGAGGCATTGAATGAGTGGGATGGCGGCATGGTGCTTGTTAGCCATGACTTTAGGCTCATTAATCAGGTGGCGCATGAGATATGGGTGTGTGCTGATCAGAAAGTGACCAAATGGGAGGGTGACATTATGGATTTCAAGAAGCATCTGAAGACGAAGGCGGGGATATTGGACTGA

Protein sequence

>Msa0546690

MVSDASKKKAAQKKAAAAAKRGGKAAAAASSKAADKVADKISDMKISDRTCTGVLCSHPLSRDIRIESLSVTFHGHDLIVDSELELNYGRRYGLLGLNGCGKSTLLTAIGMRELPIPEHMDIHHLSREIEASDMSALEAVITCDEERLQLEKEAETLAAQDDGGGEALERIYERLDALDASTAEKRAAEILDGLGFDKQMQAKKTRDFSGGWRMRISLARALFMNPTILLLDEPTNHLDLEACVWLEESLKKFERILVVISHSQDFLNGVCTNIIHMQSKKLKIYTGNYDQYVQTRGDLEENQMKQYKWEQEQIANMKEYIARFGHGSAKLARQAQSKEKTLAKMERGGLTEKVVRDQVLVFRFTDVGKLPPPVLQFSEVSFGYTPENLIYKNIDFGVDLDSRIALVGPNGAGKSTLLKLMTGDLFPSDGMVKRHNHLRIAQYHQHLAEKLDLDVPALQFMMSEYPGNEEEKMRAAIGRFGLTGKAQVMPMKNLSDGQKSRVIFAWLAYRQPQLLLLDEPTNHLDIETIDSLAEALNEWDGGMVLVSHDFRLINQVAHEIWVCADQKVTKWEGDIMDFKKHLKTKAGILD*