AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0686150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686150 MtrunA17_Chr4g0034951 97.749 311 6 1 1 310 1 311 0.0 625
Msa0686150 MtrunA17_Chr1g0155081 49.320 294 127 7 26 309 30 311 5.94e-91 273
Msa0686150 MtrunA17_Chr8g0348371 41.853 313 154 9 14 310 3 303 2.87e-66 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0686150 AT1G19400.1 55.116 303 122 7 13 310 10 303 1.58e-107 315
Msa0686150 AT1G19400.3 55.116 303 122 7 13 310 10 303 1.58e-107 315
Msa0686150 AT1G19400.2 55.116 303 122 7 13 310 10 303 1.58e-107 315
Msa0686150 AT1G75180.1 56.934 274 114 3 41 310 42 315 3.46e-107 315
Msa0686150 AT1G75180.3 56.934 274 114 3 41 310 42 315 3.46e-107 315
Msa0686150 AT1G19400.4 54.425 226 97 3 13 235 10 232 3.77e-79 241
Msa0686150 AT1G75180.2 56.477 193 82 1 41 231 42 234 7.20e-73 225
Msa0686150 AT1G75180.4 56.477 193 82 1 41 231 42 234 8.59e-72 223
Msa0686150 AT1G01500.1 42.230 296 140 10 36 310 42 327 1.56e-64 206

Find 126 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4_4:-50483363 Msa0686150:CDS
AGGTGGTAATAAATGATTTA+AGG 0.188726 4_4:-50481949 Msa0686150:three_prime_UTR
GATACCCTTCTTGAAGTTAA+TGG 0.212761 4_4:-50483638 Msa0686150:CDS
GAGAATGATGCAAATGAAAA+TGG 0.217056 4_4:-50483188 Msa0686150:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4_4:-50483159 Msa0686150:intron
CTTTAAGCTTCAATAGATTC+TGG 0.234279 4_4:-50484699 Msa0686150:five_prime_UTR
AGACATTAACATGTTGTCTT+TGG 0.250262 4_4:+50482117 None:intergenic
CATGGTTTAACGCCGGTGTT+AGG 0.255714 4_4:-50482336 Msa0686150:CDS
AAGAACAAATCTTGCAATCT+TGG 0.266859 4_4:+50484719 None:intergenic
GAGAATTCGTATAAGGTTAA+TGG 0.283457 4_4:-50483839 Msa0686150:CDS
GGAAATGGACCAAGTGAAAA+TGG 0.283915 4_4:-50483818 Msa0686150:CDS
GTTAGGGTTGGTGTTGGTAT+TGG 0.297925 4_4:-50482319 Msa0686150:CDS
GGATATATATTATATAGTTT+TGG 0.316791 4_4:-50482077 Msa0686150:three_prime_UTR
CTCATATCAACCCTCGATAA+AGG 0.321091 4_4:-50483771 Msa0686150:CDS
AGAAGGGTATCAGGATTTAA+AGG 0.331368 4_4:+50483650 None:intergenic
GACATTAACATGTTGTCTTT+GGG 0.346532 4_4:+50482118 None:intergenic
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4_4:-50483476 Msa0686150:CDS
TTAACTTCAAGAAGGGTATC+AGG 0.353127 4_4:+50483641 None:intergenic
TCGTATAAGGTTAATGGAAA+TGG 0.363071 4_4:-50483833 Msa0686150:CDS
GCGAAGATGGGGAACTTTCA+TGG 0.371984 4_4:-50482354 Msa0686150:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4_4:+50482227 None:intergenic
AGAACAAATCTTGCAATCTT+GGG 0.374948 4_4:+50484720 None:intergenic
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4_4:-50483293 Msa0686150:CDS
TAGTTCGGCCATCTTTATCT+TGG 0.380101 4_4:-50482141 Msa0686150:three_prime_UTR
CGTTACCGTGGTTAGACATA+AGG 0.381604 4_4:-50483745 Msa0686150:CDS
TGATCAACATTTATAAAGTA+AGG 0.389780 4_4:-50481817 Msa0686150:three_prime_UTR
GTTAGCGAATCAAATTCTAA+TGG 0.392431 4_4:-50483422 Msa0686150:CDS
ACAATTGAGGTCTATATTGC+CGG 0.393367 4_4:-50483305 Msa0686150:CDS
TTGTAAGCACTGATAGTATA+AGG 0.394406 4_4:-50483532 Msa0686150:CDS
TTTAAGCTTCAATAGATTCT+GGG 0.398434 4_4:-50484698 Msa0686150:five_prime_UTR
GAAAACTGAAATGTTATGTT+GGG 0.402948 4_4:+50484952 None:intergenic
GGTGGTAATAAATGATTTAA+GGG 0.404373 4_4:-50481948 Msa0686150:three_prime_UTR
GCTTACAAACGTAACTTCTT+CGG 0.405082 4_4:+50483547 None:intergenic
CGCGATGGAGAATTCGTATA+AGG 0.406902 4_4:-50483846 Msa0686150:exon
TGTTTGTCGTACATGTAATC+TGG 0.416533 4_4:+50482427 None:intergenic
TACTTTATAAATGTTGATCA+TGG 0.417685 4_4:+50481820 None:intergenic
TTTGTGTGTGAATTAATTAG+AGG 0.422085 4_4:+50484895 None:intergenic
TTCTTGAAGTTAATGGTGTT+CGG 0.422504 4_4:-50483631 Msa0686150:CDS
CTTAGTGTTTGTCTTGGAAT+CGG 0.423405 4_4:-50482295 Msa0686150:CDS
TGATTAAGTGTGAGAATCTC+AGG 0.428692 4_4:+50483677 None:intergenic
CGGAGTAGTTTATATTCTGA+TGG 0.435949 4_4:-50483611 Msa0686150:CDS
GCAATATAGACCTCAATTGT+TGG 0.437528 4_4:+50483308 None:intergenic
TTATATTCCCCTACTTATTG+AGG 0.438498 4_4:-50481969 Msa0686150:three_prime_UTR
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4_4:-50482283 Msa0686150:CDS
TTATTACCACCTCAATAAGT+AGG 0.441077 4_4:+50481960 None:intergenic
GGATTCCAACCCTTCTCAAA+AGG 0.443968 4_4:-50483589 Msa0686150:CDS
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4_4:+50483362 None:intergenic
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4_4:-50482289 Msa0686150:CDS
TGTGAGTCGAATATAATTCC+AGG 0.452189 4_4:-50483380 Msa0686150:CDS
TGAAAACTGAAATGTTATGT+TGG 0.452656 4_4:+50484951 None:intergenic
AAGAATAATATCAGATTCAA+AGG 0.458806 4_4:-50484835 Msa0686150:five_prime_UTR
ATGGTTTAACGCCGGTGTTA+GGG 0.458989 4_4:-50482335 Msa0686150:CDS
GAATTAATTAGAGGAAGAAA+AGG 0.460023 4_4:+50484904 None:intergenic
TCATATCAACCCTCGATAAA+GGG 0.470195 4_4:-50483770 Msa0686150:CDS
AGAATGATGCAAATGAAAAT+GGG 0.470852 4_4:-50483187 Msa0686150:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4_4:+50482383 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4_4:+50482242 None:intergenic
CAACCCTTCTCAAAAGGGAT+AGG 0.480345 4_4:-50483583 Msa0686150:CDS
ATTGGACTTAGTGTTTGTCT+TGG 0.481547 4_4:-50482301 Msa0686150:CDS
TATTACCACCTCAATAAGTA+GGG 0.486522 4_4:+50481961 None:intergenic
GCATACGCTGACGGCGAAGA+TGG 0.492307 4_4:-50482367 Msa0686150:CDS
CAAATTCTAATGGACAAAGT+TGG 0.492533 4_4:-50483412 Msa0686150:CDS
GTGGATTAGTGTTGCTGTGT+TGG 0.494724 4_4:-50483930 Msa0686150:five_prime_UTR
AGATAAAGATGGCCGAACTA+AGG 0.499057 4_4:+50482144 None:intergenic
GTTACCGTGGTTAGACATAA+GGG 0.505953 4_4:-50483744 Msa0686150:CDS
CATACGCTGACGGCGAAGAT+GGG 0.511131 4_4:-50482366 Msa0686150:CDS
GATTCCAACCCTTCTCAAAA+GGG 0.526688 4_4:-50483588 Msa0686150:CDS
GGAGTAGTTTATATTCTGAT+GGG 0.528712 4_4:-50483610 Msa0686150:CDS
GATCAACATTTATAAAGTAA+GGG 0.530296 4_4:-50481816 Msa0686150:three_prime_UTR
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4_4:+50483158 None:intergenic
AGAATAATATCAGATTCAAA+GGG 0.538270 4_4:-50484834 Msa0686150:five_prime_UTR
AAAGGAGAGTGTGAAATTGT+TGG 0.539146 4_4:+50484922 None:intergenic
CAAAGAAACTGAGAAAATCA+TGG 0.543052 4_4:+50481875 None:intergenic
AACCCTTCTCAAAAGGGATA+GGG 0.548703 4_4:-50483582 Msa0686150:CDS
GAGTGGTAGTGTGAAGTTTG+AGG 0.549063 4_4:-50483507 Msa0686150:CDS
GCCGGTGTTAGGGTTGGTGT+TGG 0.549235 4_4:-50482325 Msa0686150:CDS
AATCATGGTGGAGAGACTAA+TGG 0.550185 4_4:+50481890 None:intergenic
CTTGGGATGAATCAAAACAT+AGG 0.551006 4_4:+50484737 None:intergenic
CTCTACTCTTCCAACAATTG+AGG 0.551724 4_4:-50483318 Msa0686150:CDS
ATTGTTGGAAGAGTAGAGTC+TGG 0.555280 4_4:+50483323 None:intergenic
TATTCCCCTACTTATTGAGG+TGG 0.561142 4_4:-50481966 Msa0686150:three_prime_UTR
TGAGGTGGTAAGAGAAGATG+TGG 0.564391 4_4:+50484776 None:intergenic
GTGGTAATAAATGATTTAAG+GGG 0.565067 4_4:-50481947 Msa0686150:three_prime_UTR
AAATCATACCAAGGTGCCAC+CGG 0.568352 4_4:-50482250 Msa0686150:CDS
GAACACCATTAACTTCAAGA+AGG 0.569037 4_4:+50483633 None:intergenic
TTTAACGCCGGTGTTAGGGT+TGG 0.570434 4_4:-50482331 Msa0686150:CDS
ATCAAAACATAGGTTGCTAT+CGG 0.572191 4_4:+50484747 None:intergenic
GGTTGCTATCGGTTATGTTG+AGG 0.581890 4_4:+50484758 None:intergenic
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4_4:+50482231 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4_4:-50483304 Msa0686150:CDS
ATCAACATTTATAAAGTAAG+GGG 0.586146 4_4:-50481815 Msa0686150:three_prime_UTR
TGCTATCGGTTATGTTGAGG+TGG 0.587688 4_4:+50484761 None:intergenic
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4_4:-50482234 Msa0686150:CDS
TCTGGAATTGCATTCAACGC+CGG 0.588676 4_4:+50483209 None:intergenic
AAAATGGATACTCTATTGCT+AGG 0.589815 4_4:-50483802 Msa0686150:CDS
CGATAAAGGGTTCGTTACCG+TGG 0.592561 4_4:-50483757 Msa0686150:CDS
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4_4:+50483266 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4_4:+50483286 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4_4:-50483182 Msa0686150:CDS
AACACCATTAACTTCAAGAA+GGG 0.596194 4_4:+50483634 None:intergenic
CCTTCTCAAAAGGGATAGGG+TGG 0.600087 4_4:-50483579 Msa0686150:CDS
TGGTAATAAATGATTTAAGG+GGG 0.603940 4_4:-50481946 Msa0686150:three_prime_UTR
GTGGTAAGAGAAGATGTGGA+TGG 0.604928 4_4:+50484780 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4_4:-50482376 Msa0686150:CDS
GCTGTGTTGGTACAAGTCAA+TGG 0.607038 4_4:-50483917 Msa0686150:five_prime_UTR
TCTTTGGGCCAAGATAAAGA+TGG 0.610464 4_4:+50482133 None:intergenic
AAAGTAGAACTGTCTCGCGA+TGG 0.611108 4_4:-50483861 Msa0686150:exon
TTAGAATTATGTAACAGCAA+TGG 0.617960 4_4:-50483449 Msa0686150:CDS
ACCAACACCAACCCTAACAC+CGG 0.618909 4_4:+50482324 None:intergenic
AAAACTGAAATGTTATGTTG+GGG 0.627111 4_4:+50484953 None:intergenic
CGGTAACGAACCCTTTATCG+AGG 0.628590 4_4:+50483760 None:intergenic
ATACGCTGACGGCGAAGATG+GGG 0.629010 4_4:-50482365 Msa0686150:CDS
ACTGATAGTATAAGGATGAG+TGG 0.634943 4_4:-50483524 Msa0686150:CDS
CATGATGATGAGGATTATCA+CGG 0.635768 4_4:-50482406 Msa0686150:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4_4:+50483280 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4_4:+50482234 None:intergenic
GGTAACGAACCCTTTATCGA+GGG 0.642976 4_4:+50483761 None:intergenic
AACACCCTTATGTCTAACCA+CGG 0.647619 4_4:+50483740 None:intergenic
GTACGACAAACATGATGATG+AGG 0.648545 4_4:-50482416 Msa0686150:CDS
GAACTTTCATGGTTTAACGC+CGG 0.650464 4_4:-50482343 Msa0686150:CDS
ATTACCACCTCAATAAGTAG+GGG 0.652127 4_4:+50481962 None:intergenic
AAGTGTGAGAATCTCAGGAG+TGG 0.662685 4_4:+50483682 None:intergenic
GTCTTGTTCATCGAGTGCAA+AGG 0.669479 4_4:-50482098 Msa0686150:three_prime_UTR
TGATTCTTACAGTAATCACA+AGG 0.698127 4_4:-50481915 Msa0686150:three_prime_UTR
ATACTCGTTAAATCATACCA+AGG 0.711189 4_4:-50482259 Msa0686150:CDS
AGAAACTGAGAAAATCATGG+TGG 0.715746 4_4:+50481878 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAAATTTGATTTTTAATTT+TGG - chr4_4:50482184-50482203 Msa0686150:three_prime_UTR 10.0%
!! TTTACATAAAAAAATCAAAA+AGG + chr4_4:50482820-50482839 None:intergenic 10.0%
!!! GGATATTGTTTAATTTTAAT+GGG + chr4_4:50482003-50482022 None:intergenic 15.0%
!!! TGGATATTGTTTAATTTTAA+TGG + chr4_4:50482004-50482023 None:intergenic 15.0%
!!! AAATTTTGATTATTGTTATG+TGG + chr4_4:50482172-50482191 None:intergenic 15.0%
!! CAAAGAAATATAAGAATTAA+AGG + chr4_4:50482553-50482572 None:intergenic 15.0%
!! AATATAACTGTAAATTATCT+TGG - chr4_4:50484086-50484105 Msa0686150:intron 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG + chr4_4:50484256-50484275 None:intergenic 15.0%
!! GCATTTACATATAAATAATA+AGG + chr4_4:50484346-50484365 None:intergenic 15.0%
!!! GGATATATATTATATAGTTT+TGG - chr4_4:50484724-50484743 Msa0686150:five_prime_UTR 15.0%
!! AAGAATAATATCAGATTCAA+AGG - chr4_4:50481966-50481985 Msa0686150:three_prime_UTR 20.0%
!! AGAATAATATCAGATTCAAA+GGG - chr4_4:50481967-50481986 Msa0686150:three_prime_UTR 20.0%
!! CAATCAGATAAAAATAATAC+AGG + chr4_4:50482622-50482641 None:intergenic 20.0%
!!! TATTTTTATCTGATTGAGTA+AGG - chr4_4:50482626-50482645 Msa0686150:intron 20.0%
!!! ATTTATTTGTGCTTTTATGT+TGG - chr4_4:50482751-50482770 Msa0686150:intron 20.0%
!! ATTTAAATAAGCACATGAAA+AGG + chr4_4:50483691-50483710 None:intergenic 20.0%
!!! GGAATTATCTATAATTTGTT+GGG + chr4_4:50484325-50484344 None:intergenic 20.0%
!!! AGGAATTATCTATAATTTGT+TGG + chr4_4:50484326-50484345 None:intergenic 20.0%
!! TACTTTATAAATGTTGATCA+TGG + chr4_4:50484984-50485003 None:intergenic 20.0%
!! TGATCAACATTTATAAAGTA+AGG - chr4_4:50484984-50485003 Msa0686150:five_prime_UTR 20.0%
!! GATCAACATTTATAAAGTAA+GGG - chr4_4:50484985-50485004 Msa0686150:five_prime_UTR 20.0%
!! ATCAACATTTATAAAGTAAG+GGG - chr4_4:50484986-50485005 Msa0686150:five_prime_UTR 20.0%
! AAAACTGAAATGTTATGTTG+GGG + chr4_4:50481851-50481870 None:intergenic 25.0%
! GAAAACTGAAATGTTATGTT+GGG + chr4_4:50481852-50481871 None:intergenic 25.0%
! TGAAAACTGAAATGTTATGT+TGG + chr4_4:50481853-50481872 None:intergenic 25.0%
! GAATTAATTAGAGGAAGAAA+AGG + chr4_4:50481900-50481919 None:intergenic 25.0%
! TTTGTGTGTGAATTAATTAG+AGG + chr4_4:50481909-50481928 None:intergenic 25.0%
!!! TTAATTTTAATGGGAGATGT+GGG + chr4_4:50481994-50482013 None:intergenic 25.0%
!!! TTTAATTTTAATGGGAGATG+TGG + chr4_4:50481995-50482014 None:intergenic 25.0%
! TTTAAGCTTCAATAGATTCT+GGG - chr4_4:50482103-50482122 Msa0686150:three_prime_UTR 25.0%
!! TTTGAATTTTGCATTAGTGA+CGG - chr4_4:50482251-50482270 Msa0686150:CDS 25.0%
! ATTTAGACAAAAAAAACAGC+CGG + chr4_4:50482323-50482342 None:intergenic 25.0%
! AAAAATCAAAAAGGTTGTAG+AGG + chr4_4:50482811-50482830 None:intergenic 25.0%
! TTAGAATTATGTAACAGCAA+TGG - chr4_4:50483352-50483371 Msa0686150:CDS 25.0%
! AGAATGATGCAAATGAAAAT+GGG - chr4_4:50483614-50483633 Msa0686150:CDS 25.0%
! ATTCAAGTAACAAGTTAACA+AGG + chr4_4:50483743-50483762 None:intergenic 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG - chr4_4:50483861-50483880 Msa0686150:exon 25.0%
! ATATTTATGACCAAACCAAA+TGG - chr4_4:50484276-50484295 Msa0686150:intron 25.0%
! TTCTTCATAAGATTTCCATT+TGG + chr4_4:50484294-50484313 None:intergenic 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG + chr4_4:50484318-50484337 None:intergenic 25.0%
! AGGTGGTAATAAATGATTTA+AGG - chr4_4:50484852-50484871 Msa0686150:five_prime_UTR 25.0%
! GGTGGTAATAAATGATTTAA+GGG - chr4_4:50484853-50484872 Msa0686150:five_prime_UTR 25.0%
! GTGGTAATAAATGATTTAAG+GGG - chr4_4:50484854-50484873 Msa0686150:five_prime_UTR 25.0%
! TGGTAATAAATGATTTAAGG+GGG - chr4_4:50484855-50484874 Msa0686150:five_prime_UTR 25.0%
!!! AACATTTTTTGTCTGAGAAA+AGG + chr4_4:50484952-50484971 None:intergenic 25.0%
!!! TTTTCTGTTTGCTGTATAAG+GGG + chr4_4:50481828-50481847 None:intergenic 30.0%
!!! GTTTTCTGTTTGCTGTATAA+GGG + chr4_4:50481829-50481848 None:intergenic 30.0%
ATCAAAACATAGGTTGCTAT+CGG + chr4_4:50482057-50482076 None:intergenic 30.0%
AGAACAAATCTTGCAATCTT+GGG + chr4_4:50482084-50482103 None:intergenic 30.0%
AAGAACAAATCTTGCAATCT+TGG + chr4_4:50482085-50482104 None:intergenic 30.0%
CTTTAAGCTTCAATAGATTC+TGG - chr4_4:50482102-50482121 Msa0686150:three_prime_UTR 30.0%
! TTTTGGCACAAGATATTTTG+TGG - chr4_4:50482201-50482220 Msa0686150:three_prime_UTR 30.0%
CCTCAAACATATCAACTATT+CGG + chr4_4:50482435-50482454 None:intergenic 30.0%
! TATTTGTGCTTTTATGTTGG+TGG - chr4_4:50482754-50482773 Msa0686150:intron 30.0%
GAGAATTCGTATAAGGTTAA+TGG - chr4_4:50482962-50482981 Msa0686150:intron 30.0%
TCGTATAAGGTTAATGGAAA+TGG - chr4_4:50482968-50482987 Msa0686150:intron 30.0%
!! ATAGAGTATCCATTTTCACT+TGG + chr4_4:50482995-50483014 None:intergenic 30.0%
AAAATGGATACTCTATTGCT+AGG - chr4_4:50482999-50483018 Msa0686150:intron 30.0%
!!! ACATAAGGGTGTTTTATGTA+AGG - chr4_4:50483071-50483090 Msa0686150:intron 30.0%
!!! CATAAGGGTGTTTTATGTAA+GGG - chr4_4:50483072-50483091 Msa0686150:intron 30.0%
AACACCATTAACTTCAAGAA+GGG + chr4_4:50483170-50483189 None:intergenic 30.0%
TTCTTGAAGTTAATGGTGTT+CGG - chr4_4:50483170-50483189 Msa0686150:CDS 30.0%
GGAGTAGTTTATATTCTGAT+GGG - chr4_4:50483191-50483210 Msa0686150:CDS 30.0%
!! TTGTAAGCACTGATAGTATA+AGG - chr4_4:50483269-50483288 Msa0686150:CDS 30.0%
AAAGATAAGCTTCTTCTGTT+TGG - chr4_4:50483325-50483344 Msa0686150:CDS 30.0%
GTTAGCGAATCAAATTCTAA+TGG - chr4_4:50483379-50483398 Msa0686150:CDS 30.0%
! CAAATTCTAATGGACAAAGT+TGG - chr4_4:50483389-50483408 Msa0686150:CDS 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG + chr4_4:50483557-50483576 None:intergenic 30.0%
GTTCTCAAAAAAGACATACA+AGG - chr4_4:50483566-50483585 Msa0686150:CDS 30.0%
! TTTTCATTTGCATCATTCTC+TGG + chr4_4:50483613-50483632 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG - chr4_4:50483613-50483632 Msa0686150:CDS 30.0%
!! ATTGGCTCAAAGCAATTTTA+TGG - chr4_4:50483971-50483990 Msa0686150:intron 30.0%
CATAAGATTTCCATTTGGTT+TGG + chr4_4:50484289-50484308 None:intergenic 30.0%
ATACTCGTTAAATCATACCA+AGG - chr4_4:50484542-50484561 Msa0686150:intron 30.0%
AAAAACAGCTAACCTTAGTT+CGG - chr4_4:50484645-50484664 Msa0686150:five_prime_UTR 30.0%
GACATTAACATGTTGTCTTT+GGG + chr4_4:50484686-50484705 None:intergenic 30.0%
AGACATTAACATGTTGTCTT+TGG + chr4_4:50484687-50484706 None:intergenic 30.0%
TTATATTCCCCTACTTATTG+AGG - chr4_4:50484832-50484851 Msa0686150:five_prime_UTR 30.0%
TATTACCACCTCAATAAGTA+GGG + chr4_4:50484843-50484862 None:intergenic 30.0%
TTATTACCACCTCAATAAGT+AGG + chr4_4:50484844-50484863 None:intergenic 30.0%
TGATTCTTACAGTAATCACA+AGG - chr4_4:50484886-50484905 Msa0686150:five_prime_UTR 30.0%
CAAAGAAACTGAGAAAATCA+TGG + chr4_4:50484929-50484948 None:intergenic 30.0%
!!! GGTTTTCTGTTTGCTGTATA+AGG + chr4_4:50481830-50481849 None:intergenic 35.0%
! AAAGGAGAGTGTGAAATTGT+TGG + chr4_4:50481882-50481901 None:intergenic 35.0%
CTTGGGATGAATCAAAACAT+AGG + chr4_4:50482067-50482086 None:intergenic 35.0%
CAAACATATCAACTATTCGG+TGG + chr4_4:50482432-50482451 None:intergenic 35.0%
! CCGAATAGTTGATATGTTTG+AGG - chr4_4:50482432-50482451 Msa0686150:CDS 35.0%
CCTGAAAAACAGACAAAAAG+AGG + chr4_4:50482478-50482497 None:intergenic 35.0%
!! CCTCTTTTTGTCTGTTTTTC+AGG - chr4_4:50482475-50482494 Msa0686150:intron 35.0%
!!! TTTTGTCTGTTTTTCAGGTC+TGG - chr4_4:50482480-50482499 Msa0686150:intron 35.0%
!! AGTTAACTTTGTTGTTGCAG+TGG - chr4_4:50482852-50482871 Msa0686150:intron 35.0%
TCATATCAACCCTCGATAAA+GGG - chr4_4:50483031-50483050 Msa0686150:intron 35.0%
TGATTAAGTGTGAGAATCTC+AGG + chr4_4:50483127-50483146 None:intergenic 35.0%
!! AGAAGGGTATCAGGATTTAA+AGG + chr4_4:50483154-50483173 None:intergenic 35.0%
!! TTAACTTCAAGAAGGGTATC+AGG + chr4_4:50483163-50483182 None:intergenic 35.0%
GATACCCTTCTTGAAGTTAA+TGG - chr4_4:50483163-50483182 Msa0686150:CDS 35.0%
GAACACCATTAACTTCAAGA+AGG + chr4_4:50483171-50483190 None:intergenic 35.0%
CGGAGTAGTTTATATTCTGA+TGG - chr4_4:50483190-50483209 Msa0686150:CDS 35.0%
GCTTACAAACGTAACTTCTT+CGG + chr4_4:50483257-50483276 None:intergenic 35.0%
ACTGATAGTATAAGGATGAG+TGG - chr4_4:50483277-50483296 Msa0686150:CDS 35.0%
TGTGAGTCGAATATAATTCC+AGG - chr4_4:50483421-50483440 Msa0686150:CDS 35.0%
GCAATATAGACCTCAATTGT+TGG + chr4_4:50483496-50483515 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG - chr4_4:50483496-50483515 Msa0686150:CDS 35.0%
AAGGTCTTCGTTAAGATGAT+CGG + chr4_4:50483538-50483557 None:intergenic 35.0%
AAAAAGACATACAAGGAAGC+CGG - chr4_4:50483573-50483592 Msa0686150:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG - chr4_4:50483619-50483638 Msa0686150:CDS 35.0%
! GATTTGTTCAAATCCCTCAA+GGG + chr4_4:50483814-50483833 None:intergenic 35.0%
TGATATGCGATTGACAATAC+AGG - chr4_4:50483933-50483952 Msa0686150:intron 35.0%
ATTATGTTCCATCTCCTACT+AGG + chr4_4:50484058-50484077 None:intergenic 35.0%
!! TTATCTTGGCTCTTTTTCTG+AGG - chr4_4:50484100-50484119 Msa0686150:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG + chr4_4:50484232-50484251 None:intergenic 35.0%
TGTTTGTCGTACATGTAATC+TGG + chr4_4:50484377-50484396 None:intergenic 35.0%
! CATGATGATGAGGATTATCA+CGG - chr4_4:50484395-50484414 Msa0686150:intron 35.0%
ATTGGACTTAGTGTTTGTCT+TGG - chr4_4:50484500-50484519 Msa0686150:intron 35.0%
CTTAGTGTTTGTCTTGGAAT+CGG - chr4_4:50484506-50484525 Msa0686150:intron 35.0%
!!! TAGCTGTTTTTGGATCAAGA+GGG + chr4_4:50484636-50484655 None:intergenic 35.0%
!!! TTAGCTGTTTTTGGATCAAG+AGG + chr4_4:50484637-50484656 None:intergenic 35.0%
!! GAACTAAGGTTAGCTGTTTT+TGG + chr4_4:50484646-50484665 None:intergenic 35.0%
ATTACCACCTCAATAAGTAG+GGG + chr4_4:50484842-50484861 None:intergenic 35.0%
ATAAATGATTTAAGGGGGCT+TGG - chr4_4:50484860-50484879 Msa0686150:five_prime_UTR 35.0%
AGAAACTGAGAAAATCATGG+TGG + chr4_4:50484926-50484945 None:intergenic 35.0%
! TCCTCTCTTTTTCTTTCTCG+TGG - chr4_4:50481933-50481952 Msa0686150:three_prime_UTR 40.0%
TCCACGAGAAAGAAAAAGAG+AGG + chr4_4:50481937-50481956 None:intergenic 40.0%
GGAAATGGACCAAGTGAAAA+TGG - chr4_4:50482983-50483002 Msa0686150:intron 40.0%
CTCATATCAACCCTCGATAA+AGG - chr4_4:50483030-50483049 Msa0686150:intron 40.0%
GTTACCGTGGTTAGACATAA+GGG - chr4_4:50483057-50483076 Msa0686150:intron 40.0%
AACACCCTTATGTCTAACCA+CGG + chr4_4:50483064-50483083 None:intergenic 40.0%
GATTCCAACCCTTCTCAAAA+GGG - chr4_4:50483213-50483232 Msa0686150:CDS 40.0%
AACCCTTCTCAAAAGGGATA+GGG - chr4_4:50483219-50483238 Msa0686150:CDS 40.0%
TCCAGGCACAAAGTTCTTTA+AGG - chr4_4:50483438-50483457 Msa0686150:CDS 40.0%
TCCTTAAAGAACTTTGTGCC+TGG + chr4_4:50483442-50483461 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+TGG + chr4_4:50483481-50483500 None:intergenic 40.0%
CTCTACTCTTCCAACAATTG+AGG - chr4_4:50483483-50483502 Msa0686150:CDS 40.0%
CAATTGAGGTCTATATTGCC+GGG - chr4_4:50483497-50483516 Msa0686150:CDS 40.0%
! ATGCGCTGACATAGTTAAAG+AGG + chr4_4:50483717-50483736 None:intergenic 40.0%
! CGATTTGTTCAAATCCCTCA+AGG + chr4_4:50483815-50483834 None:intergenic 40.0%
AACAAATCGCTACTGTTCTG+CGG - chr4_4:50483826-50483845 Msa0686150:CDS 40.0%
GTTCTTAAATAGTGGCGCTA+TGG - chr4_4:50483869-50483888 Msa0686150:five_prime_UTR 40.0%
GCTATGGCATTGTACTTTAG+TGG - chr4_4:50483885-50483904 Msa0686150:five_prime_UTR 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG - chr4_4:50483953-50483972 Msa0686150:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG - chr4_4:50483992-50484011 Msa0686150:intron 40.0%
TTTGAGAAATAGCTGTCCTC+CGG - chr4_4:50484166-50484185 Msa0686150:intron 40.0%
! GTACGACAAACATGATGATG+AGG - chr4_4:50484385-50484404 Msa0686150:intron 40.0%
CGTATGCTGTTCTTGTGTAT+AGG + chr4_4:50484421-50484440 None:intergenic 40.0%
GAACTTTCATGGTTTAACGC+CGG - chr4_4:50484458-50484477 Msa0686150:intron 40.0%
AGAATAGCCGTCTTCTGAAT+TGG + chr4_4:50484577-50484596 None:intergenic 40.0%
AGATAAAGATGGCCGAACTA+AGG + chr4_4:50484660-50484679 None:intergenic 40.0%
TAGTTCGGCCATCTTTATCT+TGG - chr4_4:50484660-50484679 Msa0686150:five_prime_UTR 40.0%
TCTTTGGGCCAAGATAAAGA+TGG + chr4_4:50484671-50484690 None:intergenic 40.0%
TATTCCCCTACTTATTGAGG+TGG - chr4_4:50484835-50484854 Msa0686150:five_prime_UTR 40.0%
! AATCATGGTGGAGAGACTAA+TGG + chr4_4:50484914-50484933 None:intergenic 40.0%
GTGGTAAGAGAAGATGTGGA+TGG + chr4_4:50482024-50482043 None:intergenic 45.0%
TGAGGTGGTAAGAGAAGATG+TGG + chr4_4:50482028-50482047 None:intergenic 45.0%
! TGCTATCGGTTATGTTGAGG+TGG + chr4_4:50482043-50482062 None:intergenic 45.0%
! GGTTGCTATCGGTTATGTTG+AGG + chr4_4:50482046-50482065 None:intergenic 45.0%
!! GTGGATTAGTGTTGCTGTGT+TGG - chr4_4:50482871-50482890 Msa0686150:intron 45.0%
! GCTGTGTTGGTACAAGTCAA+TGG - chr4_4:50482884-50482903 Msa0686150:intron 45.0%
AAAGTAGAACTGTCTCGCGA+TGG - chr4_4:50482940-50482959 Msa0686150:intron 45.0%
CGCGATGGAGAATTCGTATA+AGG - chr4_4:50482955-50482974 Msa0686150:intron 45.0%
GGTAACGAACCCTTTATCGA+GGG + chr4_4:50483043-50483062 None:intergenic 45.0%
CGTTACCGTGGTTAGACATA+AGG - chr4_4:50483056-50483075 Msa0686150:intron 45.0%
AAGTGTGAGAATCTCAGGAG+TGG + chr4_4:50483122-50483141 None:intergenic 45.0%
GGATTCCAACCCTTCTCAAA+AGG - chr4_4:50483212-50483231 Msa0686150:CDS 45.0%
!! CTATCCCTTTTGAGAAGGGT+TGG + chr4_4:50483220-50483239 None:intergenic 45.0%
CAACCCTTCTCAAAAGGGAT+AGG - chr4_4:50483218-50483237 Msa0686150:CDS 45.0%
!! CACCCTATCCCTTTTGAGAA+GGG + chr4_4:50483224-50483243 None:intergenic 45.0%
! GAGTGGTAGTGTGAAGTTTG+AGG - chr4_4:50483294-50483313 Msa0686150:CDS 45.0%
! TCTGGAATTGCATTCAACGC+CGG + chr4_4:50483595-50483614 None:intergenic 45.0%
TGACAACACAGAGTCACACA+TGG + chr4_4:50483768-50483787 None:intergenic 45.0%
ACTATAGTGCTATGCCCTTG+AGG - chr4_4:50483797-50483816 Msa0686150:CDS 45.0%
CTATAGTGCTATGCCCTTGA+GGG - chr4_4:50483798-50483817 Msa0686150:CDS 45.0%
GACTGATGTCACAACCTAGT+AGG - chr4_4:50484041-50484060 Msa0686150:intron 45.0%
TGTCACAACCTAGTAGGAGA+TGG - chr4_4:50484047-50484066 Msa0686150:intron 45.0%
ACAAGAACAGCATACGCTGA+CGG - chr4_4:50484425-50484444 Msa0686150:intron 45.0%
! ATGGTTTAACGCCGGTGTTA+GGG - chr4_4:50484466-50484485 Msa0686150:intron 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG - chr4_4:50484482-50484501 Msa0686150:intron 45.0%
GTTTGTCTTGGAATCGGCAT+TGG - chr4_4:50484512-50484531 Msa0686150:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG - chr4_4:50484551-50484570 Msa0686150:intron 45.0%
! GTCTTGTTCATCGAGTGCAA+AGG - chr4_4:50484703-50484722 Msa0686150:five_prime_UTR 45.0%
TTGCATGTGTCGTGTTTCGC+CGG - chr4_4:50482301-50482320 Msa0686150:CDS 50.0%
CGGTAACGAACCCTTTATCG+AGG + chr4_4:50483044-50483063 None:intergenic 50.0%
CGATAAAGGGTTCGTTACCG+TGG - chr4_4:50483044-50483063 Msa0686150:intron 50.0%
!! CCACCCTATCCCTTTTGAGA+AGG + chr4_4:50483225-50483244 None:intergenic 50.0%
CCTTCTCAAAAGGGATAGGG+TGG - chr4_4:50483222-50483241 Msa0686150:CDS 50.0%
TATATTGCCGGGTCCTTCTC+TGG - chr4_4:50483508-50483527 Msa0686150:CDS 50.0%
GCGAAGATGGGGAACTTTCA+TGG - chr4_4:50484447-50484466 Msa0686150:intron 50.0%
CATGGTTTAACGCCGGTGTT+AGG - chr4_4:50484465-50484484 Msa0686150:intron 50.0%
! TTTAACGCCGGTGTTAGGGT+TGG - chr4_4:50484470-50484489 Msa0686150:intron 50.0%
ACCAACACCAACCCTAACAC+CGG + chr4_4:50484480-50484499 None:intergenic 50.0%
! CTTGGAATCGGCATTGGAGT+TGG - chr4_4:50484518-50484537 Msa0686150:intron 50.0%
TAGCCGTCTTCTGAATTGGC+CGG + chr4_4:50484573-50484592 None:intergenic 50.0%
! GATGATCGGAGTGCCAGAGA+AGG + chr4_4:50483524-50483543 None:intergenic 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG - chr4_4:50483642-50483661 Msa0686150:CDS 55.0%
ACCTGCAAAGCAAGCGCAGA+AGG + chr4_4:50483646-50483665 None:intergenic 55.0%
CATACGCTGACGGCGAAGAT+GGG - chr4_4:50484435-50484454 Msa0686150:intron 55.0%
ATACGCTGACGGCGAAGATG+GGG - chr4_4:50484436-50484455 Msa0686150:intron 55.0%
CCACCGGCCAATTCAGAAGA+CGG - chr4_4:50484567-50484586 Msa0686150:intron 55.0%
GCATACGCTGACGGCGAAGA+TGG - chr4_4:50484434-50484453 Msa0686150:intron 60.0%
!! GCCGGTGTTAGGGTTGGTGT+TGG - chr4_4:50484476-50484495 Msa0686150:intron 60.0%
TGAATTGGCCGGTGGCACCT+TGG + chr4_4:50484562-50484581 None:intergenic 60.0%
CCGTCTTCTGAATTGGCCGG+TGG + chr4_4:50484570-50484589 None:intergenic 60.0%
! CGGAGTGCCAGAGAAGGACC+CGG + chr4_4:50483518-50483537 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG + chr4_4:50484185-50484204 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG - chr4_4:50484184-50484203 Msa0686150:intron 65.0%
GCCTGCATGTGCTCTCGAGC+CGG + chr4_4:50484188-50484207 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 50481811 50485012 50481811 ID=Msa0686150;Name=Msa0686150
chr4_4 mRNA 50481811 50485012 50481811 ID=Msa0686150-mRNA-1;Parent=Msa0686150;Name=Msa0686150-mRNA-1;_AED=0.17;_eAED=0.17;_QI=499|1|1|1|0.5|0.33|3|414|310
chr4_4 exon 50481811 50482452 50481811 ID=Msa0686150-mRNA-1:exon:12938;Parent=Msa0686150-mRNA-1
chr4_4 exon 50483160 50483951 50483160 ID=Msa0686150-mRNA-1:exon:12937;Parent=Msa0686150-mRNA-1
chr4_4 exon 50484601 50485012 50484601 ID=Msa0686150-mRNA-1:exon:12936;Parent=Msa0686150-mRNA-1
chr4_4 five_prime_UTR 50484601 50485012 50484601 ID=Msa0686150-mRNA-1:five_prime_utr;Parent=Msa0686150-mRNA-1
chr4_4 five_prime_UTR 50483865 50483951 50483865 ID=Msa0686150-mRNA-1:five_prime_utr;Parent=Msa0686150-mRNA-1
chr4_4 CDS 50483160 50483864 50483160 ID=Msa0686150-mRNA-1:cds;Parent=Msa0686150-mRNA-1
chr4_4 CDS 50482225 50482452 50482225 ID=Msa0686150-mRNA-1:cds;Parent=Msa0686150-mRNA-1
chr4_4 three_prime_UTR 50481811 50482224 50481811 ID=Msa0686150-mRNA-1:three_prime_utr;Parent=Msa0686150-mRNA-1
Gene Sequence

>Msa0686150

ATGGAGAATTCGTATAAGGTTAATGGAAATGGACCAAGTGAAAATGGATACTCTATTGCTAGGCACACGCACTCATATCAACCCTCGATAAAGGGTTCGTTACCGTGGTTAGACATAAGGGTGTTTTATGTAAGGGTTTGCAAGTGTGAGCTTGATAATTCCACTCCTGAGATTCTCACACTTAATCATGTTCCTTTAAATCCTGATACCCTTCTTGAAGTTAATGGTGTTCGGAGTAGTTTATATTCTGATGGGATTCCAACCCTTCTCAAAAGGGATAGGGTGGATAGAAAATCCGAAGAAGTTACGTTTGTAAGCACTGATAGTATAAGGATGAGTGGTAGTGTGAAGTTTGAGGTGTTTGATAAAGATAAGCTTCTTCTGTTTGGTACTTTAGAATTATGTAACAGCAATGGTGTTGTTAGCGAATCAAATTCTAATGGACAAAGTTGGAGCATGAAGTGTGAGTCGAATATAATTCCAGGCACAAAGTTCTTTAAGGAGAAGCAATTGTTGTTGCCAGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGACTCCAGATTACATGTACGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGATGGGGAACTTTCATGGTTTAACGCCGGTGTTAGGGTTGGTGTTGGTATTGGACTTAGTGTTTGTCTTGGAATCGGCATTGGAGTTGGCATACTCGTTAAATCATACCAAGGTGCCACCGGCCAATTCAGAAGACGGCTATTCTAA
Protein sequence

>Msa0686150

MENSYKVNGNGPSENGYSIARHTHSYQPSIKGSLPWLDIRVFYVRVCKCELDNSTPEILTLNHVPLNPDTLLEVNGVRSSLYSDGIPTLLKRDRVDRKSEEVTFVSTDSIRMSGSVKFEVFDKDKLLLFGTLELCNSNGVVSESNSNGQSWSMKCESNIIPGTKFFKEKQLLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQTPDYMYDKHDDEDYHGLYTRTAYADGEDGELSWFNAGVRVGVGIGLSVCLGIGIGVGILVKSYQGATGQFRRRLF*