AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0551880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0551880 MtrunA17_Chr4g0037251 100.000 143 0 0 1 143 1 143 8.42e-106 297
Msa0551880 MtrunA17_Chr7g0261251 68.889 135 42 0 8 142 11 145 5.30e-71 209
Msa0551880 MtrunA17_Chr1g0187771 62.329 146 49 1 3 142 1 146 6.28e-62 187
Msa0551880 MtrunA17_Chr1g0182501 61.194 134 52 0 8 141 4 137 1.74e-60 183
Msa0551880 MtrunA17_Chr5g0397641 60.000 135 52 1 8 142 4 136 9.53e-60 181
Msa0551880 MtrunA17_Chr1g0203211 59.259 135 53 1 8 142 28 160 1.42e-57 176
Msa0551880 MtrunA17_Chr8g0380291 57.778 135 57 0 8 142 4 138 1.93e-57 175
Msa0551880 MtrunA17_Chr2g0291571 55.556 135 60 0 8 142 4 138 1.02e-56 173
Msa0551880 MtrunA17_Chr8g0386651 57.037 135 56 1 8 142 4 136 7.90e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0551880 AT2G16700.1 86.014 143 20 0 1 143 1 143 1.24e-89 257
Msa0551880 AT4G34970.1 80.851 141 27 0 3 143 1 141 2.40e-86 248
Msa0551880 AT2G16700.4 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa0551880 AT2G16700.2 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa0551880 AT2G31200.1 62.222 135 51 0 8 142 11 145 2.71e-62 188
Msa0551880 AT4G00680.1 59.701 134 54 0 8 141 4 137 2.28e-61 185
Msa0551880 AT4G00680.2 59.701 134 54 0 8 141 14 147 4.32e-61 185
Msa0551880 AT1G01750.1 59.701 134 54 0 8 141 4 137 2.02e-60 183
Msa0551880 AT1G01750.2 59.701 134 54 0 8 141 9 142 2.61e-60 183
Msa0551880 AT5G59890.1 59.701 134 54 0 8 141 4 137 1.51e-59 181
Msa0551880 AT3G46010.2 58.519 135 56 0 8 142 15 149 2.59e-59 181
Msa0551880 AT3G46010.1 58.519 135 56 0 8 142 4 138 3.30e-59 180
Msa0551880 AT4G25590.1 60.741 135 51 1 8 142 4 136 4.54e-59 179
Msa0551880 AT5G59880.1 57.037 135 58 0 8 142 4 138 3.47e-58 177
Msa0551880 AT3G46000.1 60.448 134 51 1 8 141 4 135 1.19e-57 176
Msa0551880 AT5G59890.3 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa0551880 AT5G59890.2 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa0551880 AT3G46000.2 60.448 134 51 1 8 141 23 154 1.56e-57 176
Msa0551880 AT4G25590.2 61.069 131 49 1 12 142 1 129 4.35e-57 174
Msa0551880 AT2G16700.3 77.982 109 24 0 1 109 1 109 5.09e-56 171
Msa0551880 AT5G52360.1 57.778 135 55 1 8 142 4 136 8.29e-56 171
Msa0551880 AT3G46000.3 60.769 130 49 1 12 141 1 128 1.21e-55 171
Msa0551880 AT5G59880.2 52.593 135 49 1 8 142 4 123 2.03e-48 152
Msa0551880 AT3G45990.1 40.000 135 72 3 12 141 1 131 1.83e-31 109

Find 57 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATTAAATGTTGGATTT+TGG 0.145397 4_1:+48133036 Msa0551880:three_prime_UTR
TTACTCTTTAATTATGAAAA+TGG 0.192136 4_1:+48133178 Msa0551880:three_prime_UTR
AAAATCCAACATTTAATTAA+AGG 0.207337 4_1:-48133034 None:intergenic
AATTAAGAGGGGCATCTATT+TGG 0.279597 4_1:-48132878 None:intergenic
AATGGCGATGGCTTTCAAAA+TGG 0.335809 4_1:+48131909 Msa0551880:exon
GTTGACAAGGTTGGTGGTCC+TGG 0.380021 4_1:+48132205 Msa0551880:CDS
TCTCTTATTCTTGATGCTGT+TGG 0.381519 4_1:-48132739 None:intergenic
GGATGGGTTAAGAAGAGCTT+TGG 0.389969 4_1:+48132786 Msa0551880:CDS
TATATAATAAAGAGTAAGAT+TGG 0.392525 4_1:-48132927 None:intergenic
GTCGGGTGTATGATAAGTTA+AGG 0.403852 4_1:+48133135 Msa0551880:three_prime_UTR
AAGTTATTGTATATGATTGT+CGG 0.404555 4_1:+48133117 Msa0551880:three_prime_UTR
AGAACATGTCATAGAGTCAT+TGG 0.413308 4_1:-48132984 None:intergenic
AGTTAAGGGCAACAAACCTT+TGG 0.419776 4_1:+48133150 Msa0551880:three_prime_UTR
AATGGTTGACACAAATTTGA+AGG 0.424205 4_1:-48131817 None:intergenic
GAATCTGAACAGGCTACTAC+TGG 0.427593 4_1:+48132076 Msa0551880:intron
AGTTATTGTATATGATTGTC+GGG 0.430533 4_1:+48133118 Msa0551880:three_prime_UTR
GACACAAATTTGAAGGGAAT+GGG 0.436624 4_1:-48131810 None:intergenic
TAGCTTGGCACATAATTAAG+AGG 0.439808 4_1:-48132891 None:intergenic
TCTCAGTTGGATCGGTTGCT+TGG 0.449998 4_1:-48132828 None:intergenic
TGACACAAATTTGAAGGGAA+TGG 0.472077 4_1:-48131811 None:intergenic
GCATATCTACAATCATCATT+AGG 0.474554 4_1:-48132259 None:intergenic
GGTAGAATAATTGATTAGCT+TGG 0.477263 4_1:-48132906 None:intergenic
GTAGTCCTTTAATTAAATGT+TGG 0.512500 4_1:+48133029 Msa0551880:three_prime_UTR
ATTTGAAGGGAATGGGACTC+AGG 0.515122 4_1:-48131803 None:intergenic
TCGGGTGTATGATAAGTTAA+GGG 0.515851 4_1:+48133136 Msa0551880:three_prime_UTR
GGCACCATTGTGATGTAAGT+GGG 0.522332 4_1:+48133057 Msa0551880:three_prime_UTR
AACAGGCTACTACTGGGATG+TGG 0.527418 4_1:+48132083 Msa0551880:intron
AGCAACCGATCCAACTGAGA+TGG 0.533493 4_1:+48132831 Msa0551880:CDS
ACTCTTTCATGGAGATGAAG+TGG 0.533958 4_1:+48132125 Msa0551880:CDS
ACATCGAATCCCATCTCAGT+TGG 0.534338 4_1:-48132841 None:intergenic
CATGGAGATGAAGTGGAAGA+AGG 0.543453 4_1:+48132132 Msa0551880:CDS
ATGGTTGACACAAATTTGAA+GGG 0.545737 4_1:-48131816 None:intergenic
GCAACCGATCCAACTGAGAT+GGG 0.549755 4_1:+48132832 Msa0551880:CDS
TGGCACCATTGTGATGTAAG+TGG 0.550458 4_1:+48133056 Msa0551880:three_prime_UTR
GCAAGATCTTCTTCATTGCA+TGG 0.552818 4_1:+48132326 Msa0551880:CDS
TGAAGAAGATCTTGCTCTTG+CGG 0.553047 4_1:-48132318 None:intergenic
TTAAAGAGTAATACAACCAA+AGG 0.560788 4_1:-48133166 None:intergenic
GATACTATACGCAACATCAA+AGG 0.562150 4_1:+48132765 Msa0551880:CDS
AGCTTGGCACATAATTAAGA+GGG 0.562790 4_1:-48132890 None:intergenic
GGGTTAAGAAGAGCTTTGGA+TGG 0.582337 4_1:+48132790 Msa0551880:CDS
GTGACCGTTGACAAGGTTGG+TGG 0.590709 4_1:+48132199 Msa0551880:CDS
GATGAGAAGAAGAACCAAGT+TGG 0.592365 4_1:-48131879 None:intergenic
GAATCCCATCTCAGTTGGAT+CGG 0.598606 4_1:-48132836 None:intergenic
TGAAGTGGAAGAAGGTGCAT+AGG 0.607407 4_1:+48132140 Msa0551880:CDS
CTATACGCAACATCAAAGGA+TGG 0.610779 4_1:+48132769 Msa0551880:CDS
AAGGCTTGTGACCGTTGACA+AGG 0.611864 4_1:+48132192 Msa0551880:CDS
ACAGGCTACTACTGGGATGT+GGG 0.613046 4_1:+48132084 Msa0551880:intron
TTCTTCTCATCAGATAACAA+TGG 0.614668 4_1:+48131891 Msa0551880:exon
TGGTTGAGACTTGAGAGCAA+TGG 0.618268 4_1:+48133198 Msa0551880:three_prime_UTR
TCATCAGATAACAATGGCGA+TGG 0.623350 4_1:+48131897 Msa0551880:exon
TATACGCAACATCAAAGGAT+GGG 0.626471 4_1:+48132770 Msa0551880:CDS
AATCTGAACAGGCTACTACT+GGG 0.632062 4_1:+48132077 Msa0551880:intron
AGGACCACCAACCTTGTCAA+CGG 0.633960 4_1:-48132203 None:intergenic
CTTGTGACCGTTGACAAGGT+TGG 0.653631 4_1:+48132196 Msa0551880:CDS
GCTTGGCACATAATTAAGAG+GGG 0.677123 4_1:-48132889 None:intergenic
GCACCATTGTGATGTAAGTG+GGG 0.688234 4_1:+48133058 Msa0551880:three_prime_UTR
ATACCCCACTTACATCACAA+TGG 0.734161 4_1:-48133061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGTCAATTTAGTAT+AGG - chr4_1:48132532-48132551 None:intergenic 15.0%
!!! ACTTTTTTTTTACTTTTACT+TGG + chr4_1:48132543-48132562 Msa0551880:intron 15.0%
!! TATATAATAAAGAGTAAGAT+TGG - chr4_1:48132930-48132949 None:intergenic 15.0%
!! AAAATCCAACATTTAATTAA+AGG - chr4_1:48133037-48133056 None:intergenic 15.0%
!!! TTTAATTAAATGTTGGATTT+TGG + chr4_1:48133036-48133055 Msa0551880:three_prime_UTR 15.0%
!! TTACTCTTTAATTATGAAAA+TGG + chr4_1:48133178-48133197 Msa0551880:three_prime_UTR 15.0%
!! TTTGTTTATACACATGTTTA+TGG + chr4_1:48132023-48132042 Msa0551880:intron 20.0%
!! TTTAATCCAACAATTGAAAT+TGG + chr4_1:48132420-48132439 Msa0551880:intron 20.0%
!!! TAATTTTGGGTAAAATTTTC+AGG + chr4_1:48132713-48132732 Msa0551880:intron 20.0%
!!! CTTTTTCCTTTTTCTTTTTA+TGG + chr4_1:48133007-48133026 Msa0551880:three_prime_UTR 20.0%
!! AAGTTATTGTATATGATTGT+CGG + chr4_1:48133117-48133136 Msa0551880:three_prime_UTR 20.0%
! TATGAGTGAGATGATTTAAA+AGG - chr4_1:48131945-48131964 None:intergenic 25.0%
! CATATAATATGAATCTGAAC+AGG + chr4_1:48132066-48132085 Msa0551880:intron 25.0%
! GATTAATCTAACAATTGAGA+TGG - chr4_1:48132371-48132390 None:intergenic 25.0%
! TCAATTGTTGGATTAAACAA+AGG - chr4_1:48132417-48132436 None:intergenic 25.0%
! TCACATCATATGGTATTATT+GGG - chr4_1:48132625-48132644 None:intergenic 25.0%
! ATCACATCATATGGTATTAT+TGG - chr4_1:48132626-48132645 None:intergenic 25.0%
! GTAGTCCTTTAATTAAATGT+TGG + chr4_1:48133029-48133048 Msa0551880:three_prime_UTR 25.0%
! AGTTATTGTATATGATTGTC+GGG + chr4_1:48133118-48133137 Msa0551880:three_prime_UTR 25.0%
! TTAAAGAGTAATACAACCAA+AGG - chr4_1:48133169-48133188 None:intergenic 25.0%
ATGGTTGACACAAATTTGAA+GGG - chr4_1:48131819-48131838 None:intergenic 30.0%
AATGGTTGACACAAATTTGA+AGG - chr4_1:48131820-48131839 None:intergenic 30.0%
GAAAAAAAGTAAGACCAACT+TGG + chr4_1:48131865-48131884 Msa0551880:five_prime_UTR 30.0%
TTCTTCTCATCAGATAACAA+TGG + chr4_1:48131891-48131910 Msa0551880:exon 30.0%
TCAAGATCGATGAAAAAACA+AGG + chr4_1:48132173-48132192 Msa0551880:CDS 30.0%
GCATATCTACAATCATCATT+AGG - chr4_1:48132262-48132281 None:intergenic 30.0%
CATTGAGCAAACATTGTTAT+TGG - chr4_1:48132393-48132412 None:intergenic 30.0%
TAGAGTCCAATTTCAATTGT+TGG - chr4_1:48132429-48132448 None:intergenic 30.0%
TAAATTGTACTACTAGTACC+TGG - chr4_1:48132575-48132594 None:intergenic 30.0%
TATATGAGTCTAATACAAGC+TGG + chr4_1:48132599-48132618 Msa0551880:intron 30.0%
TGTACATTCATCACATCATA+TGG - chr4_1:48132635-48132654 None:intergenic 30.0%
! TGAATGTGAGGGATAATTTT+GGG + chr4_1:48132700-48132719 Msa0551880:intron 30.0%
! GGTAGAATAATTGATTAGCT+TGG - chr4_1:48132909-48132928 None:intergenic 30.0%
GGACTACCATAAAAAGAAAA+AGG - chr4_1:48133016-48133035 None:intergenic 30.0%
!!! GCTCAAACTTTTTTCATATG+TGG + chr4_1:48133225-48133244 Msa0551880:three_prime_UTR 30.0%
GACACAAATTTGAAGGGAAT+GGG - chr4_1:48131813-48131832 None:intergenic 35.0%
TGACACAAATTTGAAGGGAA+TGG - chr4_1:48131814-48131833 None:intergenic 35.0%
TGAGTGCAAAAACTCTTTCA+TGG + chr4_1:48132114-48132133 Msa0551880:CDS 35.0%
! AGATCATCATAGTTTTCTCC+AGG - chr4_1:48132226-48132245 None:intergenic 35.0%
ACACACAATTAGATCGTCTA+CGG - chr4_1:48132465-48132484 None:intergenic 35.0%
ACGATCTAATTGTGTGTGTT+TGG + chr4_1:48132468-48132487 Msa0551880:intron 35.0%
! ACTTTTACTTGGTAAGTACC+AGG + chr4_1:48132554-48132573 Msa0551880:intron 35.0%
! TGATGATGAATGTGAATGTG+AGG + chr4_1:48132688-48132707 Msa0551880:intron 35.0%
GATGATGAATGTGAATGTGA+GGG + chr4_1:48132689-48132708 Msa0551880:intron 35.0%
! GTGAATGTGAGGGATAATTT+TGG + chr4_1:48132699-48132718 Msa0551880:intron 35.0%
TCTCTTATTCTTGATGCTGT+TGG - chr4_1:48132742-48132761 None:intergenic 35.0%
GATACTATACGCAACATCAA+AGG + chr4_1:48132765-48132784 Msa0551880:CDS 35.0%
TATACGCAACATCAAAGGAT+GGG + chr4_1:48132770-48132789 Msa0551880:CDS 35.0%
!! AATTAAGAGGGGCATCTATT+TGG - chr4_1:48132881-48132900 None:intergenic 35.0%
AGCTTGGCACATAATTAAGA+GGG - chr4_1:48132893-48132912 None:intergenic 35.0%
TAGCTTGGCACATAATTAAG+AGG - chr4_1:48132894-48132913 None:intergenic 35.0%
AGAACATGTCATAGAGTCAT+TGG - chr4_1:48132987-48133006 None:intergenic 35.0%
TCGGGTGTATGATAAGTTAA+GGG + chr4_1:48133136-48133155 Msa0551880:three_prime_UTR 35.0%
GATGAGAAGAAGAACCAAGT+TGG - chr4_1:48131882-48131901 None:intergenic 40.0%
TCATCAGATAACAATGGCGA+TGG + chr4_1:48131897-48131916 Msa0551880:exon 40.0%
AATGGCGATGGCTTTCAAAA+TGG + chr4_1:48131909-48131928 Msa0551880:exon 40.0%
AATCTGAACAGGCTACTACT+GGG + chr4_1:48132077-48132096 Msa0551880:intron 40.0%
ACTCTTTCATGGAGATGAAG+TGG + chr4_1:48132125-48132144 Msa0551880:CDS 40.0%
TGAAGAAGATCTTGCTCTTG+CGG - chr4_1:48132321-48132340 None:intergenic 40.0%
GCAAGATCTTCTTCATTGCA+TGG + chr4_1:48132326-48132345 Msa0551880:CDS 40.0%
CTATACGCAACATCAAAGGA+TGG + chr4_1:48132769-48132788 Msa0551880:CDS 40.0%
GCTTGGCACATAATTAAGAG+GGG - chr4_1:48132892-48132911 None:intergenic 40.0%
ATACCCCACTTACATCACAA+TGG - chr4_1:48133064-48133083 None:intergenic 40.0%
GTCGGGTGTATGATAAGTTA+AGG + chr4_1:48133135-48133154 Msa0551880:three_prime_UTR 40.0%
AGTTAAGGGCAACAAACCTT+TGG + chr4_1:48133150-48133169 Msa0551880:three_prime_UTR 40.0%
GAATCTGAACAGGCTACTAC+TGG + chr4_1:48132076-48132095 Msa0551880:intron 45.0%
CATGGAGATGAAGTGGAAGA+AGG + chr4_1:48132132-48132151 Msa0551880:CDS 45.0%
! TGAAGTGGAAGAAGGTGCAT+AGG + chr4_1:48132140-48132159 Msa0551880:CDS 45.0%
AATTGGACTCTACAAAGCCG+TGG + chr4_1:48132437-48132456 Msa0551880:intron 45.0%
!! GGATGGGTTAAGAAGAGCTT+TGG + chr4_1:48132786-48132805 Msa0551880:CDS 45.0%
!! GGGTTAAGAAGAGCTTTGGA+TGG + chr4_1:48132790-48132809 Msa0551880:CDS 45.0%
GAATCCCATCTCAGTTGGAT+CGG - chr4_1:48132839-48132858 None:intergenic 45.0%
ACATCGAATCCCATCTCAGT+TGG - chr4_1:48132844-48132863 None:intergenic 45.0%
TGGCACCATTGTGATGTAAG+TGG + chr4_1:48133056-48133075 Msa0551880:three_prime_UTR 45.0%
GGCACCATTGTGATGTAAGT+GGG + chr4_1:48133057-48133076 Msa0551880:three_prime_UTR 45.0%
GCACCATTGTGATGTAAGTG+GGG + chr4_1:48133058-48133077 Msa0551880:three_prime_UTR 45.0%
TGGTTGAGACTTGAGAGCAA+TGG + chr4_1:48133198-48133217 Msa0551880:three_prime_UTR 45.0%
!!! AATTTATGATATTTTTATAA+TGG - chr4_1:48131838-48131857 None:intergenic 5.0%
AACAGGCTACTACTGGGATG+TGG + chr4_1:48132083-48132102 Msa0551880:intron 50.0%
ACAGGCTACTACTGGGATGT+GGG + chr4_1:48132084-48132103 Msa0551880:intron 50.0%
AAGGCTTGTGACCGTTGACA+AGG + chr4_1:48132192-48132211 Msa0551880:CDS 50.0%
CTTGTGACCGTTGACAAGGT+TGG + chr4_1:48132196-48132215 Msa0551880:CDS 50.0%
AGGACCACCAACCTTGTCAA+CGG - chr4_1:48132206-48132225 None:intergenic 50.0%
TCTCAGTTGGATCGGTTGCT+TGG - chr4_1:48132831-48132850 None:intergenic 50.0%
AGCAACCGATCCAACTGAGA+TGG + chr4_1:48132831-48132850 Msa0551880:CDS 50.0%
GCAACCGATCCAACTGAGAT+GGG + chr4_1:48132832-48132851 Msa0551880:CDS 50.0%
GTGACCGTTGACAAGGTTGG+TGG + chr4_1:48132199-48132218 Msa0551880:CDS 55.0%
!! GTTGACAAGGTTGGTGGTCC+TGG + chr4_1:48132205-48132224 Msa0551880:CDS 55.0%
TTAGATCGTCTACGGCGCCA+CGG - chr4_1:48132457-48132476 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 48131806 48133265 48131806 ID=Msa0551880;Name=Msa0551880
chr4_1 mRNA 48131806 48133265 48131806 ID=Msa0551880-mRNA-1;Parent=Msa0551880;Name=Msa0551880-mRNA-1;_AED=0.03;_eAED=0.03;_QI=104|1|1|1|1|1|3|380|143
chr4_1 exon 48131806 48131930 48131806 ID=Msa0551880-mRNA-1:exon:13119;Parent=Msa0551880-mRNA-1
chr4_1 exon 48132088 48132347 48132088 ID=Msa0551880-mRNA-1:exon:13120;Parent=Msa0551880-mRNA-1
chr4_1 exon 48132735 48133265 48132735 ID=Msa0551880-mRNA-1:exon:13121;Parent=Msa0551880-mRNA-1
chr4_1 five_prime_UTR 48131806 48131909 48131806 ID=Msa0551880-mRNA-1:five_prime_utr;Parent=Msa0551880-mRNA-1
chr4_1 CDS 48131910 48131930 48131910 ID=Msa0551880-mRNA-1:cds;Parent=Msa0551880-mRNA-1
chr4_1 CDS 48132088 48132347 48132088 ID=Msa0551880-mRNA-1:cds;Parent=Msa0551880-mRNA-1
chr4_1 CDS 48132735 48132885 48132735 ID=Msa0551880-mRNA-1:cds;Parent=Msa0551880-mRNA-1
chr4_1 three_prime_UTR 48132886 48133265 48132886 ID=Msa0551880-mRNA-1:three_prime_utr;Parent=Msa0551880-mRNA-1
Gene Sequence

>Msa0551880

ATGGCGATGGCTTTCAAAATGGCTACTACTGGGATGTGGGTAACAGATGAGTGCAAAAACTCTTTCATGGAGATGAAGTGGAAGAAGGTGCATAGGTACATAGTTTTCAAGATCGATGAAAAAACAAGGCTTGTGACCGTTGACAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAATGATGATTGTAGATATGCTGTCTTTGATTTTGATTTTGTCACTGTTGACAACTGCCGCAAGAGCAAGATCTTCTTCATTGCATGGTCACCAACAGCATCAAGAATAAGAGAAAAGATACTATACGCAACATCAAAGGATGGGTTAAGAAGAGCTTTGGATGGAATCTCATATGAACTCCAAGCAACCGATCCAACTGAGATGGGATTCGATGTAATTCAAGACAGAGCCAAATAG

Protein sequence

>Msa0551880

MAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEKTRLVTVDKVGGPGENYDDLAASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIREKILYATSKDGLRRALDGISYELQATDPTEMGFDVIQDRAK*