AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1305700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305700 MtrunA17_Chr4g0037251 100.000 143 0 0 1 143 1 143 8.42e-106 297
Msa1305700 MtrunA17_Chr7g0261251 68.889 135 42 0 8 142 11 145 5.30e-71 209
Msa1305700 MtrunA17_Chr1g0187771 62.329 146 49 1 3 142 1 146 6.28e-62 187
Msa1305700 MtrunA17_Chr1g0182501 61.194 134 52 0 8 141 4 137 1.74e-60 183
Msa1305700 MtrunA17_Chr5g0397641 60.000 135 52 1 8 142 4 136 9.53e-60 181
Msa1305700 MtrunA17_Chr1g0203211 59.259 135 53 1 8 142 28 160 1.42e-57 176
Msa1305700 MtrunA17_Chr8g0380291 57.778 135 57 0 8 142 4 138 1.93e-57 175
Msa1305700 MtrunA17_Chr2g0291571 55.556 135 60 0 8 142 4 138 1.02e-56 173
Msa1305700 MtrunA17_Chr8g0386651 57.037 135 56 1 8 142 4 136 7.90e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1305700 AT2G16700.1 86.014 143 20 0 1 143 1 143 1.24e-89 257
Msa1305700 AT4G34970.1 80.851 141 27 0 3 143 1 141 2.40e-86 248
Msa1305700 AT2G16700.4 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa1305700 AT2G16700.2 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa1305700 AT2G31200.1 62.222 135 51 0 8 142 11 145 2.71e-62 188
Msa1305700 AT4G00680.1 59.701 134 54 0 8 141 4 137 2.28e-61 185
Msa1305700 AT4G00680.2 59.701 134 54 0 8 141 14 147 4.32e-61 185
Msa1305700 AT1G01750.1 59.701 134 54 0 8 141 4 137 2.02e-60 183
Msa1305700 AT1G01750.2 59.701 134 54 0 8 141 9 142 2.61e-60 183
Msa1305700 AT5G59890.1 59.701 134 54 0 8 141 4 137 1.51e-59 181
Msa1305700 AT3G46010.2 58.519 135 56 0 8 142 15 149 2.59e-59 181
Msa1305700 AT3G46010.1 58.519 135 56 0 8 142 4 138 3.30e-59 180
Msa1305700 AT4G25590.1 60.741 135 51 1 8 142 4 136 4.54e-59 179
Msa1305700 AT5G59880.1 57.037 135 58 0 8 142 4 138 3.47e-58 177
Msa1305700 AT3G46000.1 60.448 134 51 1 8 141 4 135 1.19e-57 176
Msa1305700 AT5G59890.3 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa1305700 AT5G59890.2 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa1305700 AT3G46000.2 60.448 134 51 1 8 141 23 154 1.56e-57 176
Msa1305700 AT4G25590.2 61.069 131 49 1 12 142 1 129 4.35e-57 174
Msa1305700 AT2G16700.3 77.982 109 24 0 1 109 1 109 5.09e-56 171
Msa1305700 AT5G52360.1 57.778 135 55 1 8 142 4 136 8.29e-56 171
Msa1305700 AT3G46000.3 60.769 130 49 1 12 141 1 128 1.21e-55 171
Msa1305700 AT5G59880.2 52.593 135 49 1 8 142 4 123 2.03e-48 152
Msa1305700 AT3G45990.1 40.000 135 72 3 12 141 1 131 1.83e-31 109

Find 57 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATTAAATGTTGGATTT+TGG 0.145397 8_3:+69260272 Msa1305700:three_prime_UTR
TTACTCTTTAATTATGAAAA+TGG 0.192136 8_3:+69260414 Msa1305700:three_prime_UTR
AAAATCCAACATTTAATTAA+AGG 0.207337 8_3:-69260270 None:intergenic
AATTAAGAGGGGCATCTATT+TGG 0.279597 8_3:-69260114 None:intergenic
AATGGCGATGGCTTTCAAAA+TGG 0.335809 8_3:+69259148 Msa1305700:exon
GTTGACAAGGTTGGTGGTCC+TGG 0.380021 8_3:+69259444 Msa1305700:CDS
TCTCTTATTCTTGATGCTGT+TGG 0.381519 8_3:-69259975 None:intergenic
GGATGGGTTAAGAAGAGCTT+TGG 0.389969 8_3:+69260022 Msa1305700:CDS
GTCGGGTGTATGATAAGTTA+AGG 0.403852 8_3:+69260371 Msa1305700:three_prime_UTR
AAGTTATTGTATATGATTGT+CGG 0.404555 8_3:+69260353 Msa1305700:three_prime_UTR
AGAACATGTCATAGAGTCAT+TGG 0.413308 8_3:-69260220 None:intergenic
AGTTAAGGGCAACAAACCTT+TGG 0.419776 8_3:+69260386 Msa1305700:three_prime_UTR
AATGGTTGACACAAATTTGA+AGG 0.424205 8_3:-69259056 None:intergenic
GAATCTGAACAGGCTACTAC+TGG 0.427593 8_3:+69259315 Msa1305700:intron
AGTTATTGTATATGATTGTC+GGG 0.430533 8_3:+69260354 Msa1305700:three_prime_UTR
GACACAAATTTGAAGGGAAT+GGG 0.436624 8_3:-69259049 None:intergenic
TAGCTTGGCACATAATTAAG+AGG 0.439808 8_3:-69260127 None:intergenic
TCTCAGTTGGATCGGTTGCT+TGG 0.449998 8_3:-69260064 None:intergenic
TGACACAAATTTGAAGGGAA+TGG 0.472077 8_3:-69259050 None:intergenic
GCATATCTACAATCATCATT+AGG 0.474554 8_3:-69259498 None:intergenic
GTAGTCCTTTAATTAAATGT+TGG 0.512500 8_3:+69260265 Msa1305700:three_prime_UTR
ATTTGAAGGGAATGGGACTC+AGG 0.515122 8_3:-69259042 None:intergenic
TCGGGTGTATGATAAGTTAA+GGG 0.515851 8_3:+69260372 Msa1305700:three_prime_UTR
GGTAGAATAATTGATTAGCT+TGG 0.516735 8_3:-69260142 None:intergenic
TATATAATAAAGAGTAAGAT+CGG 0.519052 8_3:-69260163 None:intergenic
GGCACCATTGTGATGTAAGT+GGG 0.522332 8_3:+69260293 Msa1305700:three_prime_UTR
AACAGGCTACTACTGGGATG+TGG 0.527418 8_3:+69259322 Msa1305700:intron
AGCAACCGATCCAACTGAGA+TGG 0.533493 8_3:+69260067 Msa1305700:CDS
ACTCTTTCATGGAGATGAAG+TGG 0.533958 8_3:+69259364 Msa1305700:CDS
ACATCGAATCCCATCTCAGT+TGG 0.534338 8_3:-69260077 None:intergenic
CATGGAGATGAAGTGGAAGA+AGG 0.543453 8_3:+69259371 Msa1305700:CDS
ATGGTTGACACAAATTTGAA+GGG 0.545737 8_3:-69259055 None:intergenic
GCAACCGATCCAACTGAGAT+GGG 0.549755 8_3:+69260068 Msa1305700:CDS
TGGCACCATTGTGATGTAAG+TGG 0.550458 8_3:+69260292 Msa1305700:three_prime_UTR
GCAAGATCTTCTTCATTGCA+TGG 0.552818 8_3:+69259565 Msa1305700:CDS
TGAAGAAGATCTTGCTCTTG+CGG 0.553047 8_3:-69259557 None:intergenic
TTAAAGAGTAATACAACCAA+AGG 0.560788 8_3:-69260402 None:intergenic
GATACTATACGCAACATCAA+AGG 0.562150 8_3:+69260001 Msa1305700:CDS
AGCTTGGCACATAATTAAGA+GGG 0.562790 8_3:-69260126 None:intergenic
GGGTTAAGAAGAGCTTTGGA+TGG 0.582337 8_3:+69260026 Msa1305700:CDS
GTGACCGTTGACAAGGTTGG+TGG 0.590709 8_3:+69259438 Msa1305700:CDS
GATGAGAAGAAGAACCAAGT+TGG 0.592365 8_3:-69259118 None:intergenic
GAATCCCATCTCAGTTGGAT+CGG 0.598606 8_3:-69260072 None:intergenic
TGAAGTGGAAGAAGGTGCAT+AGG 0.607407 8_3:+69259379 Msa1305700:CDS
CTATACGCAACATCAAAGGA+TGG 0.610779 8_3:+69260005 Msa1305700:CDS
AAGGCTTGTGACCGTTGACA+AGG 0.611864 8_3:+69259431 Msa1305700:CDS
ACAGGCTACTACTGGGATGT+GGG 0.613046 8_3:+69259323 Msa1305700:intron
TTCTTCTCATCAGATAACAA+TGG 0.614668 8_3:+69259130 Msa1305700:exon
TGGTTGAGACTTGAGAGCAA+TGG 0.618268 8_3:+69260434 Msa1305700:three_prime_UTR
TCATCAGATAACAATGGCGA+TGG 0.623350 8_3:+69259136 Msa1305700:exon
TATACGCAACATCAAAGGAT+GGG 0.626471 8_3:+69260006 Msa1305700:CDS
AATCTGAACAGGCTACTACT+GGG 0.632062 8_3:+69259316 Msa1305700:intron
AGGACCACCAACCTTGTCAA+CGG 0.633960 8_3:-69259442 None:intergenic
CTTGTGACCGTTGACAAGGT+TGG 0.653631 8_3:+69259435 Msa1305700:CDS
GCTTGGCACATAATTAAGAG+GGG 0.677123 8_3:-69260125 None:intergenic
GCACCATTGTGATGTAAGTG+GGG 0.688234 8_3:+69260294 Msa1305700:three_prime_UTR
ATACCCCACTTACATCACAA+TGG 0.734161 8_3:-69260297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGTCAATTTAGTAT+AGG - chr8_3:69259771-69259790 None:intergenic 15.0%
!!! ACTTTTTTTTTACTTTTACT+TGG + chr8_3:69259782-69259801 Msa1305700:intron 15.0%
!! TATATAATAAAGAGTAAGAT+CGG - chr8_3:69260166-69260185 None:intergenic 15.0%
!! AAAATCCAACATTTAATTAA+AGG - chr8_3:69260273-69260292 None:intergenic 15.0%
!!! TTTAATTAAATGTTGGATTT+TGG + chr8_3:69260272-69260291 Msa1305700:three_prime_UTR 15.0%
!! TTACTCTTTAATTATGAAAA+TGG + chr8_3:69260414-69260433 Msa1305700:three_prime_UTR 15.0%
!! AAAAAAAGTCAATTTAGTAT+AGG - chr8_3:69259771-69259790 None:intergenic 15.0%
!!! ACTTTTTTTTTACTTTTACT+TGG + chr8_3:69259782-69259801 Msa1305700:intron 15.0%
!! TATATAATAAAGAGTAAGAT+CGG - chr8_3:69260166-69260185 None:intergenic 15.0%
!! AAAATCCAACATTTAATTAA+AGG - chr8_3:69260273-69260292 None:intergenic 15.0%
!!! TTTAATTAAATGTTGGATTT+TGG + chr8_3:69260272-69260291 Msa1305700:three_prime_UTR 15.0%
!! TTACTCTTTAATTATGAAAA+TGG + chr8_3:69260414-69260433 Msa1305700:three_prime_UTR 15.0%
!! TTTGTTTATACACATGTTTA+TGG + chr8_3:69259262-69259281 Msa1305700:intron 20.0%
!! TTTAATCCAACAATTGAAAT+TGG + chr8_3:69259659-69259678 Msa1305700:intron 20.0%
!!! TAATTTTGGGTAAAATTTTC+AGG + chr8_3:69259949-69259968 Msa1305700:intron 20.0%
!!! CTTTTTCCTTTTTCTTTTTA+TGG + chr8_3:69260243-69260262 Msa1305700:three_prime_UTR 20.0%
!! AAGTTATTGTATATGATTGT+CGG + chr8_3:69260353-69260372 Msa1305700:three_prime_UTR 20.0%
!! TTTGTTTATACACATGTTTA+TGG + chr8_3:69259262-69259281 Msa1305700:intron 20.0%
!! TTTAATCCAACAATTGAAAT+TGG + chr8_3:69259659-69259678 Msa1305700:intron 20.0%
!!! TAATTTTGGGTAAAATTTTC+AGG + chr8_3:69259949-69259968 Msa1305700:intron 20.0%
!!! CTTTTTCCTTTTTCTTTTTA+TGG + chr8_3:69260243-69260262 Msa1305700:three_prime_UTR 20.0%
!! AAGTTATTGTATATGATTGT+CGG + chr8_3:69260353-69260372 Msa1305700:three_prime_UTR 20.0%
! TATGAGTGAGATGATTTAAA+AGG - chr8_3:69259184-69259203 None:intergenic 25.0%
! CATATAATATGAATCTGAAC+AGG + chr8_3:69259305-69259324 Msa1305700:intron 25.0%
! GATTAATCTAACAATTGAGA+TGG - chr8_3:69259610-69259629 None:intergenic 25.0%
! TCAATTGTTGGATTAAACAA+AGG - chr8_3:69259656-69259675 None:intergenic 25.0%
! TAAATTGTACTACTAGTACT+TGG - chr8_3:69259814-69259833 None:intergenic 25.0%
! TCACATCATATGGTATTATT+GGG - chr8_3:69259864-69259883 None:intergenic 25.0%
! ATCACATCATATGGTATTAT+TGG - chr8_3:69259865-69259884 None:intergenic 25.0%
! GTAGTCCTTTAATTAAATGT+TGG + chr8_3:69260265-69260284 Msa1305700:three_prime_UTR 25.0%
! AGTTATTGTATATGATTGTC+GGG + chr8_3:69260354-69260373 Msa1305700:three_prime_UTR 25.0%
! TTAAAGAGTAATACAACCAA+AGG - chr8_3:69260405-69260424 None:intergenic 25.0%
! TATGAGTGAGATGATTTAAA+AGG - chr8_3:69259184-69259203 None:intergenic 25.0%
! CATATAATATGAATCTGAAC+AGG + chr8_3:69259305-69259324 Msa1305700:intron 25.0%
! GATTAATCTAACAATTGAGA+TGG - chr8_3:69259610-69259629 None:intergenic 25.0%
! TCAATTGTTGGATTAAACAA+AGG - chr8_3:69259656-69259675 None:intergenic 25.0%
! TAAATTGTACTACTAGTACT+TGG - chr8_3:69259814-69259833 None:intergenic 25.0%
! TCACATCATATGGTATTATT+GGG - chr8_3:69259864-69259883 None:intergenic 25.0%
! ATCACATCATATGGTATTAT+TGG - chr8_3:69259865-69259884 None:intergenic 25.0%
! GTAGTCCTTTAATTAAATGT+TGG + chr8_3:69260265-69260284 Msa1305700:three_prime_UTR 25.0%
! AGTTATTGTATATGATTGTC+GGG + chr8_3:69260354-69260373 Msa1305700:three_prime_UTR 25.0%
! TTAAAGAGTAATACAACCAA+AGG - chr8_3:69260405-69260424 None:intergenic 25.0%
ATGGTTGACACAAATTTGAA+GGG - chr8_3:69259058-69259077 None:intergenic 30.0%
AATGGTTGACACAAATTTGA+AGG - chr8_3:69259059-69259078 None:intergenic 30.0%
GAAAAAAAGTAAGACCAACT+TGG + chr8_3:69259104-69259123 Msa1305700:five_prime_UTR 30.0%
TTCTTCTCATCAGATAACAA+TGG + chr8_3:69259130-69259149 Msa1305700:exon 30.0%
TCAAGATCGATGAAAAAACA+AGG + chr8_3:69259412-69259431 Msa1305700:CDS 30.0%
GCATATCTACAATCATCATT+AGG - chr8_3:69259501-69259520 None:intergenic 30.0%
CATTGAGCAAACATTGTTAT+TGG - chr8_3:69259632-69259651 None:intergenic 30.0%
TAGAGTCCAATTTCAATTGT+TGG - chr8_3:69259668-69259687 None:intergenic 30.0%
ACACACAATTATATCGTCTA+CGG - chr8_3:69259704-69259723 None:intergenic 30.0%
ACGATATAATTGTGTGTGTT+TGG + chr8_3:69259707-69259726 Msa1305700:intron 30.0%
TATATGAGTCTAATACAAGC+TGG + chr8_3:69259838-69259857 Msa1305700:intron 30.0%
TGTACATTCATCACATCATA+TGG - chr8_3:69259874-69259893 None:intergenic 30.0%
! TGAATGTGAGGGATAATTTT+GGG + chr8_3:69259936-69259955 Msa1305700:intron 30.0%
! GGTAGAATAATTGATTAGCT+TGG - chr8_3:69260145-69260164 None:intergenic 30.0%
GGACTACCATAAAAAGAAAA+AGG - chr8_3:69260252-69260271 None:intergenic 30.0%
!!! GCTCAAACTTTTTTCATATG+TGG + chr8_3:69260461-69260480 Msa1305700:three_prime_UTR 30.0%
ATGGTTGACACAAATTTGAA+GGG - chr8_3:69259058-69259077 None:intergenic 30.0%
AATGGTTGACACAAATTTGA+AGG - chr8_3:69259059-69259078 None:intergenic 30.0%
GAAAAAAAGTAAGACCAACT+TGG + chr8_3:69259104-69259123 Msa1305700:five_prime_UTR 30.0%
TTCTTCTCATCAGATAACAA+TGG + chr8_3:69259130-69259149 Msa1305700:exon 30.0%
TCAAGATCGATGAAAAAACA+AGG + chr8_3:69259412-69259431 Msa1305700:CDS 30.0%
GCATATCTACAATCATCATT+AGG - chr8_3:69259501-69259520 None:intergenic 30.0%
CATTGAGCAAACATTGTTAT+TGG - chr8_3:69259632-69259651 None:intergenic 30.0%
TAGAGTCCAATTTCAATTGT+TGG - chr8_3:69259668-69259687 None:intergenic 30.0%
ACACACAATTATATCGTCTA+CGG - chr8_3:69259704-69259723 None:intergenic 30.0%
ACGATATAATTGTGTGTGTT+TGG + chr8_3:69259707-69259726 Msa1305700:intron 30.0%
TATATGAGTCTAATACAAGC+TGG + chr8_3:69259838-69259857 Msa1305700:intron 30.0%
TGTACATTCATCACATCATA+TGG - chr8_3:69259874-69259893 None:intergenic 30.0%
! TGAATGTGAGGGATAATTTT+GGG + chr8_3:69259936-69259955 Msa1305700:intron 30.0%
! GGTAGAATAATTGATTAGCT+TGG - chr8_3:69260145-69260164 None:intergenic 30.0%
GGACTACCATAAAAAGAAAA+AGG - chr8_3:69260252-69260271 None:intergenic 30.0%
!!! GCTCAAACTTTTTTCATATG+TGG + chr8_3:69260461-69260480 Msa1305700:three_prime_UTR 30.0%
GACACAAATTTGAAGGGAAT+GGG - chr8_3:69259052-69259071 None:intergenic 35.0%
TGACACAAATTTGAAGGGAA+TGG - chr8_3:69259053-69259072 None:intergenic 35.0%
TGAGTGCAAAAACTCTTTCA+TGG + chr8_3:69259353-69259372 Msa1305700:CDS 35.0%
! AGATCATCATAGTTTTCTCC+AGG - chr8_3:69259465-69259484 None:intergenic 35.0%
! TGATGATGAATGTGAATGTG+AGG + chr8_3:69259924-69259943 Msa1305700:intron 35.0%
GATGATGAATGTGAATGTGA+GGG + chr8_3:69259925-69259944 Msa1305700:intron 35.0%
! GTGAATGTGAGGGATAATTT+TGG + chr8_3:69259935-69259954 Msa1305700:intron 35.0%
TCTCTTATTCTTGATGCTGT+TGG - chr8_3:69259978-69259997 None:intergenic 35.0%
GATACTATACGCAACATCAA+AGG + chr8_3:69260001-69260020 Msa1305700:CDS 35.0%
TATACGCAACATCAAAGGAT+GGG + chr8_3:69260006-69260025 Msa1305700:CDS 35.0%
!! AATTAAGAGGGGCATCTATT+TGG - chr8_3:69260117-69260136 None:intergenic 35.0%
AGCTTGGCACATAATTAAGA+GGG - chr8_3:69260129-69260148 None:intergenic 35.0%
TAGCTTGGCACATAATTAAG+AGG - chr8_3:69260130-69260149 None:intergenic 35.0%
AGAACATGTCATAGAGTCAT+TGG - chr8_3:69260223-69260242 None:intergenic 35.0%
TCGGGTGTATGATAAGTTAA+GGG + chr8_3:69260372-69260391 Msa1305700:three_prime_UTR 35.0%
GACACAAATTTGAAGGGAAT+GGG - chr8_3:69259052-69259071 None:intergenic 35.0%
TGACACAAATTTGAAGGGAA+TGG - chr8_3:69259053-69259072 None:intergenic 35.0%
TGAGTGCAAAAACTCTTTCA+TGG + chr8_3:69259353-69259372 Msa1305700:CDS 35.0%
! AGATCATCATAGTTTTCTCC+AGG - chr8_3:69259465-69259484 None:intergenic 35.0%
! TGATGATGAATGTGAATGTG+AGG + chr8_3:69259924-69259943 Msa1305700:intron 35.0%
GATGATGAATGTGAATGTGA+GGG + chr8_3:69259925-69259944 Msa1305700:intron 35.0%
! GTGAATGTGAGGGATAATTT+TGG + chr8_3:69259935-69259954 Msa1305700:intron 35.0%
TCTCTTATTCTTGATGCTGT+TGG - chr8_3:69259978-69259997 None:intergenic 35.0%
GATACTATACGCAACATCAA+AGG + chr8_3:69260001-69260020 Msa1305700:CDS 35.0%
TATACGCAACATCAAAGGAT+GGG + chr8_3:69260006-69260025 Msa1305700:CDS 35.0%
!! AATTAAGAGGGGCATCTATT+TGG - chr8_3:69260117-69260136 None:intergenic 35.0%
AGCTTGGCACATAATTAAGA+GGG - chr8_3:69260129-69260148 None:intergenic 35.0%
TAGCTTGGCACATAATTAAG+AGG - chr8_3:69260130-69260149 None:intergenic 35.0%
AGAACATGTCATAGAGTCAT+TGG - chr8_3:69260223-69260242 None:intergenic 35.0%
TCGGGTGTATGATAAGTTAA+GGG + chr8_3:69260372-69260391 Msa1305700:three_prime_UTR 35.0%
GATGAGAAGAAGAACCAAGT+TGG - chr8_3:69259121-69259140 None:intergenic 40.0%
TCATCAGATAACAATGGCGA+TGG + chr8_3:69259136-69259155 Msa1305700:exon 40.0%
AATGGCGATGGCTTTCAAAA+TGG + chr8_3:69259148-69259167 Msa1305700:exon 40.0%
AATCTGAACAGGCTACTACT+GGG + chr8_3:69259316-69259335 Msa1305700:intron 40.0%
ACTCTTTCATGGAGATGAAG+TGG + chr8_3:69259364-69259383 Msa1305700:CDS 40.0%
TGAAGAAGATCTTGCTCTTG+CGG - chr8_3:69259560-69259579 None:intergenic 40.0%
GCAAGATCTTCTTCATTGCA+TGG + chr8_3:69259565-69259584 Msa1305700:CDS 40.0%
CTATACGCAACATCAAAGGA+TGG + chr8_3:69260005-69260024 Msa1305700:CDS 40.0%
GCTTGGCACATAATTAAGAG+GGG - chr8_3:69260128-69260147 None:intergenic 40.0%
ATACCCCACTTACATCACAA+TGG - chr8_3:69260300-69260319 None:intergenic 40.0%
GTCGGGTGTATGATAAGTTA+AGG + chr8_3:69260371-69260390 Msa1305700:three_prime_UTR 40.0%
AGTTAAGGGCAACAAACCTT+TGG + chr8_3:69260386-69260405 Msa1305700:three_prime_UTR 40.0%
GATGAGAAGAAGAACCAAGT+TGG - chr8_3:69259121-69259140 None:intergenic 40.0%
TCATCAGATAACAATGGCGA+TGG + chr8_3:69259136-69259155 Msa1305700:exon 40.0%
AATGGCGATGGCTTTCAAAA+TGG + chr8_3:69259148-69259167 Msa1305700:exon 40.0%
AATCTGAACAGGCTACTACT+GGG + chr8_3:69259316-69259335 Msa1305700:intron 40.0%
ACTCTTTCATGGAGATGAAG+TGG + chr8_3:69259364-69259383 Msa1305700:CDS 40.0%
TGAAGAAGATCTTGCTCTTG+CGG - chr8_3:69259560-69259579 None:intergenic 40.0%
GCAAGATCTTCTTCATTGCA+TGG + chr8_3:69259565-69259584 Msa1305700:CDS 40.0%
CTATACGCAACATCAAAGGA+TGG + chr8_3:69260005-69260024 Msa1305700:CDS 40.0%
GCTTGGCACATAATTAAGAG+GGG - chr8_3:69260128-69260147 None:intergenic 40.0%
ATACCCCACTTACATCACAA+TGG - chr8_3:69260300-69260319 None:intergenic 40.0%
GTCGGGTGTATGATAAGTTA+AGG + chr8_3:69260371-69260390 Msa1305700:three_prime_UTR 40.0%
AGTTAAGGGCAACAAACCTT+TGG + chr8_3:69260386-69260405 Msa1305700:three_prime_UTR 40.0%
GAATCTGAACAGGCTACTAC+TGG + chr8_3:69259315-69259334 Msa1305700:intron 45.0%
CATGGAGATGAAGTGGAAGA+AGG + chr8_3:69259371-69259390 Msa1305700:CDS 45.0%
! TGAAGTGGAAGAAGGTGCAT+AGG + chr8_3:69259379-69259398 Msa1305700:CDS 45.0%
AATTGGACTCTACAAAGCCG+TGG + chr8_3:69259676-69259695 Msa1305700:intron 45.0%
!! GGATGGGTTAAGAAGAGCTT+TGG + chr8_3:69260022-69260041 Msa1305700:CDS 45.0%
!! GGGTTAAGAAGAGCTTTGGA+TGG + chr8_3:69260026-69260045 Msa1305700:CDS 45.0%
GAATCCCATCTCAGTTGGAT+CGG - chr8_3:69260075-69260094 None:intergenic 45.0%
ACATCGAATCCCATCTCAGT+TGG - chr8_3:69260080-69260099 None:intergenic 45.0%
TGGCACCATTGTGATGTAAG+TGG + chr8_3:69260292-69260311 Msa1305700:three_prime_UTR 45.0%
GGCACCATTGTGATGTAAGT+GGG + chr8_3:69260293-69260312 Msa1305700:three_prime_UTR 45.0%
GCACCATTGTGATGTAAGTG+GGG + chr8_3:69260294-69260313 Msa1305700:three_prime_UTR 45.0%
TGGTTGAGACTTGAGAGCAA+TGG + chr8_3:69260434-69260453 Msa1305700:three_prime_UTR 45.0%
ATTTGAAGGGAATGGGACTC+TGG - chr8_3:69259045-69259064 None:intergenic 45.0%
GAATCTGAACAGGCTACTAC+TGG + chr8_3:69259315-69259334 Msa1305700:intron 45.0%
CATGGAGATGAAGTGGAAGA+AGG + chr8_3:69259371-69259390 Msa1305700:CDS 45.0%
! TGAAGTGGAAGAAGGTGCAT+AGG + chr8_3:69259379-69259398 Msa1305700:CDS 45.0%
AATTGGACTCTACAAAGCCG+TGG + chr8_3:69259676-69259695 Msa1305700:intron 45.0%
!! GGATGGGTTAAGAAGAGCTT+TGG + chr8_3:69260022-69260041 Msa1305700:CDS 45.0%
!! GGGTTAAGAAGAGCTTTGGA+TGG + chr8_3:69260026-69260045 Msa1305700:CDS 45.0%
GAATCCCATCTCAGTTGGAT+CGG - chr8_3:69260075-69260094 None:intergenic 45.0%
ACATCGAATCCCATCTCAGT+TGG - chr8_3:69260080-69260099 None:intergenic 45.0%
TGGCACCATTGTGATGTAAG+TGG + chr8_3:69260292-69260311 Msa1305700:three_prime_UTR 45.0%
GGCACCATTGTGATGTAAGT+GGG + chr8_3:69260293-69260312 Msa1305700:three_prime_UTR 45.0%
GCACCATTGTGATGTAAGTG+GGG + chr8_3:69260294-69260313 Msa1305700:three_prime_UTR 45.0%
TGGTTGAGACTTGAGAGCAA+TGG + chr8_3:69260434-69260453 Msa1305700:three_prime_UTR 45.0%
!!! AATTTATGATATTTTTATAA+TGG - chr8_3:69259077-69259096 None:intergenic 5.0%
!!! AATTTATGATATTTTTATAA+TGG - chr8_3:69259077-69259096 None:intergenic 5.0%
AACAGGCTACTACTGGGATG+TGG + chr8_3:69259322-69259341 Msa1305700:intron 50.0%
ACAGGCTACTACTGGGATGT+GGG + chr8_3:69259323-69259342 Msa1305700:intron 50.0%
AAGGCTTGTGACCGTTGACA+AGG + chr8_3:69259431-69259450 Msa1305700:CDS 50.0%
CTTGTGACCGTTGACAAGGT+TGG + chr8_3:69259435-69259454 Msa1305700:CDS 50.0%
AGGACCACCAACCTTGTCAA+CGG - chr8_3:69259445-69259464 None:intergenic 50.0%
TTATATCGTCTACGGCGCCA+CGG - chr8_3:69259696-69259715 None:intergenic 50.0%
TCTCAGTTGGATCGGTTGCT+TGG - chr8_3:69260067-69260086 None:intergenic 50.0%
AGCAACCGATCCAACTGAGA+TGG + chr8_3:69260067-69260086 Msa1305700:CDS 50.0%
GCAACCGATCCAACTGAGAT+GGG + chr8_3:69260068-69260087 Msa1305700:CDS 50.0%
AACAGGCTACTACTGGGATG+TGG + chr8_3:69259322-69259341 Msa1305700:intron 50.0%
ACAGGCTACTACTGGGATGT+GGG + chr8_3:69259323-69259342 Msa1305700:intron 50.0%
AAGGCTTGTGACCGTTGACA+AGG + chr8_3:69259431-69259450 Msa1305700:CDS 50.0%
CTTGTGACCGTTGACAAGGT+TGG + chr8_3:69259435-69259454 Msa1305700:CDS 50.0%
AGGACCACCAACCTTGTCAA+CGG - chr8_3:69259445-69259464 None:intergenic 50.0%
TTATATCGTCTACGGCGCCA+CGG - chr8_3:69259696-69259715 None:intergenic 50.0%
TCTCAGTTGGATCGGTTGCT+TGG - chr8_3:69260067-69260086 None:intergenic 50.0%
AGCAACCGATCCAACTGAGA+TGG + chr8_3:69260067-69260086 Msa1305700:CDS 50.0%
GCAACCGATCCAACTGAGAT+GGG + chr8_3:69260068-69260087 Msa1305700:CDS 50.0%
GTGACCGTTGACAAGGTTGG+TGG + chr8_3:69259438-69259457 Msa1305700:CDS 55.0%
!! GTTGACAAGGTTGGTGGTCC+TGG + chr8_3:69259444-69259463 Msa1305700:CDS 55.0%
GTGACCGTTGACAAGGTTGG+TGG + chr8_3:69259438-69259457 Msa1305700:CDS 55.0%
!! GTTGACAAGGTTGGTGGTCC+TGG + chr8_3:69259444-69259463 Msa1305700:CDS 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 69259045 69260501 69259045 ID=Msa1305700;Name=Msa1305700
chr8_3 mRNA 69259045 69260501 69259045 ID=Msa1305700-mRNA-1;Parent=Msa1305700;Name=Msa1305700-mRNA-1;_AED=0.03;_eAED=0.03;_QI=104|1|1|1|1|1|3|380|143
chr8_3 exon 69259045 69259169 69259045 ID=Msa1305700-mRNA-1:exon:21474;Parent=Msa1305700-mRNA-1
chr8_3 exon 69259327 69259586 69259327 ID=Msa1305700-mRNA-1:exon:21475;Parent=Msa1305700-mRNA-1
chr8_3 exon 69259971 69260501 69259971 ID=Msa1305700-mRNA-1:exon:21476;Parent=Msa1305700-mRNA-1
chr8_3 five_prime_UTR 69259045 69259148 69259045 ID=Msa1305700-mRNA-1:five_prime_utr;Parent=Msa1305700-mRNA-1
chr8_3 CDS 69259149 69259169 69259149 ID=Msa1305700-mRNA-1:cds;Parent=Msa1305700-mRNA-1
chr8_3 CDS 69259327 69259586 69259327 ID=Msa1305700-mRNA-1:cds;Parent=Msa1305700-mRNA-1
chr8_3 CDS 69259971 69260121 69259971 ID=Msa1305700-mRNA-1:cds;Parent=Msa1305700-mRNA-1
chr8_3 three_prime_UTR 69260122 69260501 69260122 ID=Msa1305700-mRNA-1:three_prime_utr;Parent=Msa1305700-mRNA-1
Gene Sequence

>Msa1305700

ATGGCGATGGCTTTCAAAATGGCTACTACTGGGATGTGGGTAACAGATGAGTGCAAAAACTCTTTCATGGAGATGAAGTGGAAGAAGGTGCATAGGTACATAGTTTTCAAGATCGATGAAAAAACAAGGCTTGTGACCGTTGACAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAATGATGATTGTAGATATGCTGTCTTTGATTTTGATTTTGTCACTGTTGACAACTGCCGCAAGAGCAAGATCTTCTTCATTGCATGGTCACCAACAGCATCAAGAATAAGAGAAAAGATACTATACGCAACATCAAAGGATGGGTTAAGAAGAGCTTTGGATGGAATCTCATATGAACTCCAAGCAACCGATCCAACTGAGATGGGATTCGATGTAATTCAAGACAGAGCCAAATAG
Protein sequence

>Msa1305700

MAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEKTRLVTVDKVGGPGENYDDLAASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIREKILYATSKDGLRRALDGISYELQATDPTEMGFDVIQDRAK*