AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0687030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687030 MtrunA17_Chr4g0037251 100.000 143 0 0 1 143 1 143 8.42e-106 297
Msa0687030 MtrunA17_Chr7g0261251 68.889 135 42 0 8 142 11 145 5.30e-71 209
Msa0687030 MtrunA17_Chr1g0187771 62.329 146 49 1 3 142 1 146 6.28e-62 187
Msa0687030 MtrunA17_Chr1g0182501 61.194 134 52 0 8 141 4 137 1.74e-60 183
Msa0687030 MtrunA17_Chr5g0397641 60.000 135 52 1 8 142 4 136 9.53e-60 181
Msa0687030 MtrunA17_Chr1g0203211 59.259 135 53 1 8 142 28 160 1.42e-57 176
Msa0687030 MtrunA17_Chr8g0380291 57.778 135 57 0 8 142 4 138 1.93e-57 175
Msa0687030 MtrunA17_Chr2g0291571 55.556 135 60 0 8 142 4 138 1.02e-56 173
Msa0687030 MtrunA17_Chr8g0386651 57.037 135 56 1 8 142 4 136 7.90e-56 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0687030 AT2G16700.1 86.014 143 20 0 1 143 1 143 1.24e-89 257
Msa0687030 AT4G34970.1 80.851 141 27 0 3 143 1 141 2.40e-86 248
Msa0687030 AT2G16700.4 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa0687030 AT2G16700.2 84.848 132 20 0 12 143 1 132 5.02e-81 235
Msa0687030 AT2G31200.1 62.222 135 51 0 8 142 11 145 2.71e-62 188
Msa0687030 AT4G00680.1 59.701 134 54 0 8 141 4 137 2.28e-61 185
Msa0687030 AT4G00680.2 59.701 134 54 0 8 141 14 147 4.32e-61 185
Msa0687030 AT1G01750.1 59.701 134 54 0 8 141 4 137 2.02e-60 183
Msa0687030 AT1G01750.2 59.701 134 54 0 8 141 9 142 2.61e-60 183
Msa0687030 AT5G59890.1 59.701 134 54 0 8 141 4 137 1.51e-59 181
Msa0687030 AT3G46010.2 58.519 135 56 0 8 142 15 149 2.59e-59 181
Msa0687030 AT3G46010.1 58.519 135 56 0 8 142 4 138 3.30e-59 180
Msa0687030 AT4G25590.1 60.741 135 51 1 8 142 4 136 4.54e-59 179
Msa0687030 AT5G59880.1 57.037 135 58 0 8 142 4 138 3.47e-58 177
Msa0687030 AT3G46000.1 60.448 134 51 1 8 141 4 135 1.19e-57 176
Msa0687030 AT5G59890.3 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa0687030 AT5G59890.2 60.000 130 52 0 12 141 1 130 1.50e-57 176
Msa0687030 AT3G46000.2 60.448 134 51 1 8 141 23 154 1.56e-57 176
Msa0687030 AT4G25590.2 61.069 131 49 1 12 142 1 129 4.35e-57 174
Msa0687030 AT2G16700.3 77.982 109 24 0 1 109 1 109 5.09e-56 171
Msa0687030 AT5G52360.1 57.778 135 55 1 8 142 4 136 8.29e-56 171
Msa0687030 AT3G46000.3 60.769 130 49 1 12 141 1 128 1.21e-55 171
Msa0687030 AT5G59880.2 52.593 135 49 1 8 142 4 123 2.03e-48 152
Msa0687030 AT3G45990.1 40.000 135 72 3 12 141 1 131 1.83e-31 109

Find 57 sgRNAs with CRISPR-Local

Find 177 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTAATTAAATGTTGGATTT+TGG 0.145397 4_4:-51699991 Msa0687030:three_prime_UTR
TTACTCTTTAATTATGAAAA+TGG 0.192136 4_4:-51699849 Msa0687030:three_prime_UTR
AAAATCCAACATTTAATTAA+AGG 0.207337 4_4:+51699993 None:intergenic
AATTAAGAGGGGCATCTATT+TGG 0.279597 4_4:+51700149 None:intergenic
AATGGCGATGGCTTTCAAAA+TGG 0.335809 4_4:-51701115 Msa0687030:intron
GTTGACAAGGTTGGTGGTCC+TGG 0.380021 4_4:-51700819 Msa0687030:CDS
TCTCTTATTCTTGATGCTGT+TGG 0.381519 4_4:+51700288 None:intergenic
GGATGGGTTAAGAAGAGCTT+TGG 0.389969 4_4:-51700241 Msa0687030:CDS
GTCGGGTGTATGATAAGTTA+AGG 0.403852 4_4:-51699892 Msa0687030:three_prime_UTR
AAGTTATTGTATATGATTGT+CGG 0.404555 4_4:-51699910 Msa0687030:three_prime_UTR
AGAACATGTCATAGAGTCAT+TGG 0.413308 4_4:+51700043 None:intergenic
AGTTAAGGGCAACAAACCTT+TGG 0.419776 4_4:-51699877 Msa0687030:three_prime_UTR
AATGGTTGACACAAATTTGA+AGG 0.424205 4_4:+51701207 None:intergenic
GAATCTGAACAGGCTACTAC+TGG 0.427593 4_4:-51700948 Msa0687030:intron
AGTTATTGTATATGATTGTC+GGG 0.430533 4_4:-51699909 Msa0687030:three_prime_UTR
GACACAAATTTGAAGGGAAT+GGG 0.436624 4_4:+51701214 None:intergenic
TAGCTTGGCACATAATTAAG+AGG 0.439808 4_4:+51700136 None:intergenic
TCTCAGTTGGATCGGTTGCT+TGG 0.449998 4_4:+51700199 None:intergenic
TGACACAAATTTGAAGGGAA+TGG 0.472077 4_4:+51701213 None:intergenic
GCATATCTACAATCATCATT+AGG 0.474554 4_4:+51700765 None:intergenic
GTAGTCCTTTAATTAAATGT+TGG 0.512500 4_4:-51699998 Msa0687030:three_prime_UTR
ATTTGAAGGGAATGGGACTC+AGG 0.515122 4_4:+51701221 None:intergenic
TCGGGTGTATGATAAGTTAA+GGG 0.515851 4_4:-51699891 Msa0687030:three_prime_UTR
GGTAGAATAATTGATTAGCT+TGG 0.516735 4_4:+51700121 None:intergenic
TATATAATAAAGAGTAAGAT+CGG 0.519052 4_4:+51700100 None:intergenic
GGCACCATTGTGATGTAAGT+GGG 0.522332 4_4:-51699970 Msa0687030:three_prime_UTR
AACAGGCTACTACTGGGATG+TGG 0.527418 4_4:-51700941 Msa0687030:intron
AGCAACCGATCCAACTGAGA+TGG 0.533493 4_4:-51700196 Msa0687030:CDS
ACTCTTTCATGGAGATGAAG+TGG 0.533958 4_4:-51700899 Msa0687030:CDS
ACATCGAATCCCATCTCAGT+TGG 0.534338 4_4:+51700186 None:intergenic
CATGGAGATGAAGTGGAAGA+AGG 0.543453 4_4:-51700892 Msa0687030:CDS
ATGGTTGACACAAATTTGAA+GGG 0.545737 4_4:+51701208 None:intergenic
GCAACCGATCCAACTGAGAT+GGG 0.549755 4_4:-51700195 Msa0687030:CDS
TGGCACCATTGTGATGTAAG+TGG 0.550458 4_4:-51699971 Msa0687030:three_prime_UTR
GCAAGATCTTCTTCATTGCA+TGG 0.552818 4_4:-51700698 Msa0687030:intron
TGAAGAAGATCTTGCTCTTG+CGG 0.553047 4_4:+51700706 None:intergenic
TTAAAGAGTAATACAACCAA+AGG 0.560788 4_4:+51699861 None:intergenic
GATACTATACGCAACATCAA+AGG 0.562150 4_4:-51700262 Msa0687030:CDS
AGCTTGGCACATAATTAAGA+GGG 0.562790 4_4:+51700137 None:intergenic
GGGTTAAGAAGAGCTTTGGA+TGG 0.582337 4_4:-51700237 Msa0687030:CDS
GTGACCGTTGACAAGGTTGG+TGG 0.590709 4_4:-51700825 Msa0687030:CDS
GATGAGAAGAAGAACCAAGT+TGG 0.592365 4_4:+51701145 None:intergenic
GAATCCCATCTCAGTTGGAT+CGG 0.598606 4_4:+51700191 None:intergenic
TGAAGTGGAAGAAGGTGCAT+AGG 0.607407 4_4:-51700884 Msa0687030:CDS
CTATACGCAACATCAAAGGA+TGG 0.610779 4_4:-51700258 Msa0687030:CDS
AAGGCTTGTGACCGTTGACA+AGG 0.611864 4_4:-51700832 Msa0687030:CDS
ACAGGCTACTACTGGGATGT+GGG 0.613046 4_4:-51700940 Msa0687030:intron
TTCTTCTCATCAGATAACAA+TGG 0.614668 4_4:-51701133 Msa0687030:exon
TGGTTGAGACTTGAGAGCAA+TGG 0.618268 4_4:-51699829 Msa0687030:three_prime_UTR
TCATCAGATAACAATGGCGA+TGG 0.623350 4_4:-51701127 Msa0687030:exon
TATACGCAACATCAAAGGAT+GGG 0.626471 4_4:-51700257 Msa0687030:CDS
AATCTGAACAGGCTACTACT+GGG 0.632062 4_4:-51700947 Msa0687030:intron
AGGACCACCAACCTTGTCAA+CGG 0.633960 4_4:+51700821 None:intergenic
CTTGTGACCGTTGACAAGGT+TGG 0.653631 4_4:-51700828 Msa0687030:CDS
GCTTGGCACATAATTAAGAG+GGG 0.677123 4_4:+51700138 None:intergenic
GCACCATTGTGATGTAAGTG+GGG 0.688234 4_4:-51699969 Msa0687030:three_prime_UTR
ATACCCCACTTACATCACAA+TGG 0.734161 4_4:+51699966 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAGTCAATTTAGTAT+AGG + chr4_4:51700510-51700529 None:intergenic 15.0%
!!! ACTTTTTTTTTACTTTTACT+TGG - chr4_4:51700521-51700540 Msa0687030:intron 15.0%
!! TATATAATAAAGAGTAAGAT+CGG + chr4_4:51700905-51700924 None:intergenic 15.0%
!! AAAATCCAACATTTAATTAA+AGG + chr4_4:51701012-51701031 None:intergenic 15.0%
!!! TTTAATTAAATGTTGGATTT+TGG - chr4_4:51701011-51701030 Msa0687030:intron 15.0%
!! TTACTCTTTAATTATGAAAA+TGG - chr4_4:51701153-51701172 Msa0687030:five_prime_UTR 15.0%
!! AAAAAAAGTCAATTTAGTAT+AGG + chr4_4:51700510-51700529 None:intergenic 15.0%
!!! ACTTTTTTTTTACTTTTACT+TGG - chr4_4:51700521-51700540 Msa0687030:intron 15.0%
!! TATATAATAAAGAGTAAGAT+CGG + chr4_4:51700905-51700924 None:intergenic 15.0%
!! AAAATCCAACATTTAATTAA+AGG + chr4_4:51701012-51701031 None:intergenic 15.0%
!!! TTTAATTAAATGTTGGATTT+TGG - chr4_4:51701011-51701030 Msa0687030:intron 15.0%
!! TTACTCTTTAATTATGAAAA+TGG - chr4_4:51701153-51701172 Msa0687030:five_prime_UTR 15.0%
!! TTTGTTTATACACATGTTTA+TGG - chr4_4:51700001-51700020 Msa0687030:three_prime_UTR 20.0%
!! TTTAATCCAACAATTGAAAT+TGG - chr4_4:51700398-51700417 Msa0687030:intron 20.0%
!!! TAATTTTGGGTAAAATTTTC+AGG - chr4_4:51700688-51700707 Msa0687030:intron 20.0%
!!! CTTTTTCCTTTTTCTTTTTA+TGG - chr4_4:51700982-51701001 Msa0687030:intron 20.0%
!! AAGTTATTGTATATGATTGT+CGG - chr4_4:51701092-51701111 Msa0687030:intron 20.0%
!! TTTGTTTATACACATGTTTA+TGG - chr4_4:51700001-51700020 Msa0687030:three_prime_UTR 20.0%
!! TTTAATCCAACAATTGAAAT+TGG - chr4_4:51700398-51700417 Msa0687030:intron 20.0%
!!! TAATTTTGGGTAAAATTTTC+AGG - chr4_4:51700688-51700707 Msa0687030:intron 20.0%
!!! CTTTTTCCTTTTTCTTTTTA+TGG - chr4_4:51700982-51701001 Msa0687030:intron 20.0%
!! AAGTTATTGTATATGATTGT+CGG - chr4_4:51701092-51701111 Msa0687030:intron 20.0%
! TATGAGTGAGATGATTTAAA+AGG + chr4_4:51699923-51699942 None:intergenic 25.0%
! CATATAATATGAATCTGAAC+AGG - chr4_4:51700044-51700063 Msa0687030:three_prime_UTR 25.0%
! GATTAATCTAACAATTGAGA+TGG + chr4_4:51700349-51700368 None:intergenic 25.0%
! TCAATTGTTGGATTAAACAA+AGG + chr4_4:51700395-51700414 None:intergenic 25.0%
! TAAATTGTACTACTAGTACT+TGG + chr4_4:51700553-51700572 None:intergenic 25.0%
! TCACATCATATGGTATTATT+GGG + chr4_4:51700603-51700622 None:intergenic 25.0%
! ATCACATCATATGGTATTAT+TGG + chr4_4:51700604-51700623 None:intergenic 25.0%
! GTAGTCCTTTAATTAAATGT+TGG - chr4_4:51701004-51701023 Msa0687030:intron 25.0%
! AGTTATTGTATATGATTGTC+GGG - chr4_4:51701093-51701112 Msa0687030:intron 25.0%
! TTAAAGAGTAATACAACCAA+AGG + chr4_4:51701144-51701163 None:intergenic 25.0%
! TATGAGTGAGATGATTTAAA+AGG + chr4_4:51699923-51699942 None:intergenic 25.0%
! CATATAATATGAATCTGAAC+AGG - chr4_4:51700044-51700063 Msa0687030:three_prime_UTR 25.0%
! GATTAATCTAACAATTGAGA+TGG + chr4_4:51700349-51700368 None:intergenic 25.0%
! TCAATTGTTGGATTAAACAA+AGG + chr4_4:51700395-51700414 None:intergenic 25.0%
! TAAATTGTACTACTAGTACT+TGG + chr4_4:51700553-51700572 None:intergenic 25.0%
! TCACATCATATGGTATTATT+GGG + chr4_4:51700603-51700622 None:intergenic 25.0%
! ATCACATCATATGGTATTAT+TGG + chr4_4:51700604-51700623 None:intergenic 25.0%
! GTAGTCCTTTAATTAAATGT+TGG - chr4_4:51701004-51701023 Msa0687030:intron 25.0%
! AGTTATTGTATATGATTGTC+GGG - chr4_4:51701093-51701112 Msa0687030:intron 25.0%
! TTAAAGAGTAATACAACCAA+AGG + chr4_4:51701144-51701163 None:intergenic 25.0%
ATGGTTGACACAAATTTGAA+GGG + chr4_4:51699797-51699816 None:intergenic 30.0%
AATGGTTGACACAAATTTGA+AGG + chr4_4:51699798-51699817 None:intergenic 30.0%
GAAAAAAAGTAAGACCAACT+TGG - chr4_4:51699843-51699862 Msa0687030:three_prime_UTR 30.0%
TTCTTCTCATCAGATAACAA+TGG - chr4_4:51699869-51699888 Msa0687030:three_prime_UTR 30.0%
TCAAGATCGATGAAAAAACA+AGG - chr4_4:51700151-51700170 Msa0687030:exon 30.0%
GCATATCTACAATCATCATT+AGG + chr4_4:51700240-51700259 None:intergenic 30.0%
CATTGAGCAAACATTGTTAT+TGG + chr4_4:51700371-51700390 None:intergenic 30.0%
TAGAGTCCAATTTCAATTGT+TGG + chr4_4:51700407-51700426 None:intergenic 30.0%
ACACACAATTATATCGTCTA+CGG + chr4_4:51700443-51700462 None:intergenic 30.0%
ACGATATAATTGTGTGTGTT+TGG - chr4_4:51700446-51700465 Msa0687030:intron 30.0%
TATATGAGTCTAATACAAGC+TGG - chr4_4:51700577-51700596 Msa0687030:intron 30.0%
TGTACATTCATCACATCATA+TGG + chr4_4:51700613-51700632 None:intergenic 30.0%
! TGAATGTGAGGGATAATTTT+GGG - chr4_4:51700675-51700694 Msa0687030:intron 30.0%
! GGTAGAATAATTGATTAGCT+TGG + chr4_4:51700884-51700903 None:intergenic 30.0%
GGACTACCATAAAAAGAAAA+AGG + chr4_4:51700991-51701010 None:intergenic 30.0%
!!! GCTCAAACTTTTTTCATATG+TGG - chr4_4:51701200-51701219 Msa0687030:five_prime_UTR 30.0%
ATGGTTGACACAAATTTGAA+GGG + chr4_4:51699797-51699816 None:intergenic 30.0%
AATGGTTGACACAAATTTGA+AGG + chr4_4:51699798-51699817 None:intergenic 30.0%
GAAAAAAAGTAAGACCAACT+TGG - chr4_4:51699843-51699862 Msa0687030:three_prime_UTR 30.0%
TTCTTCTCATCAGATAACAA+TGG - chr4_4:51699869-51699888 Msa0687030:three_prime_UTR 30.0%
TCAAGATCGATGAAAAAACA+AGG - chr4_4:51700151-51700170 Msa0687030:exon 30.0%
GCATATCTACAATCATCATT+AGG + chr4_4:51700240-51700259 None:intergenic 30.0%
CATTGAGCAAACATTGTTAT+TGG + chr4_4:51700371-51700390 None:intergenic 30.0%
TAGAGTCCAATTTCAATTGT+TGG + chr4_4:51700407-51700426 None:intergenic 30.0%
ACACACAATTATATCGTCTA+CGG + chr4_4:51700443-51700462 None:intergenic 30.0%
ACGATATAATTGTGTGTGTT+TGG - chr4_4:51700446-51700465 Msa0687030:intron 30.0%
TATATGAGTCTAATACAAGC+TGG - chr4_4:51700577-51700596 Msa0687030:intron 30.0%
TGTACATTCATCACATCATA+TGG + chr4_4:51700613-51700632 None:intergenic 30.0%
! TGAATGTGAGGGATAATTTT+GGG - chr4_4:51700675-51700694 Msa0687030:intron 30.0%
! GGTAGAATAATTGATTAGCT+TGG + chr4_4:51700884-51700903 None:intergenic 30.0%
GGACTACCATAAAAAGAAAA+AGG + chr4_4:51700991-51701010 None:intergenic 30.0%
!!! GCTCAAACTTTTTTCATATG+TGG - chr4_4:51701200-51701219 Msa0687030:five_prime_UTR 30.0%
GACACAAATTTGAAGGGAAT+GGG + chr4_4:51699791-51699810 None:intergenic 35.0%
TGACACAAATTTGAAGGGAA+TGG + chr4_4:51699792-51699811 None:intergenic 35.0%
TGAGTGCAAAAACTCTTTCA+TGG - chr4_4:51700092-51700111 Msa0687030:three_prime_UTR 35.0%
! AGATCATCATAGTTTTCTCC+AGG + chr4_4:51700204-51700223 None:intergenic 35.0%
! TGATGATGAATGTGAATGTG+AGG - chr4_4:51700663-51700682 Msa0687030:intron 35.0%
GATGATGAATGTGAATGTGA+GGG - chr4_4:51700664-51700683 Msa0687030:intron 35.0%
! GTGAATGTGAGGGATAATTT+TGG - chr4_4:51700674-51700693 Msa0687030:intron 35.0%
TCTCTTATTCTTGATGCTGT+TGG + chr4_4:51700717-51700736 None:intergenic 35.0%
GATACTATACGCAACATCAA+AGG - chr4_4:51700740-51700759 Msa0687030:CDS 35.0%
TATACGCAACATCAAAGGAT+GGG - chr4_4:51700745-51700764 Msa0687030:CDS 35.0%
!! AATTAAGAGGGGCATCTATT+TGG + chr4_4:51700856-51700875 None:intergenic 35.0%
AGCTTGGCACATAATTAAGA+GGG + chr4_4:51700868-51700887 None:intergenic 35.0%
TAGCTTGGCACATAATTAAG+AGG + chr4_4:51700869-51700888 None:intergenic 35.0%
AGAACATGTCATAGAGTCAT+TGG + chr4_4:51700962-51700981 None:intergenic 35.0%
TCGGGTGTATGATAAGTTAA+GGG - chr4_4:51701111-51701130 Msa0687030:intron 35.0%
GACACAAATTTGAAGGGAAT+GGG + chr4_4:51699791-51699810 None:intergenic 35.0%
TGACACAAATTTGAAGGGAA+TGG + chr4_4:51699792-51699811 None:intergenic 35.0%
TGAGTGCAAAAACTCTTTCA+TGG - chr4_4:51700092-51700111 Msa0687030:three_prime_UTR 35.0%
! AGATCATCATAGTTTTCTCC+AGG + chr4_4:51700204-51700223 None:intergenic 35.0%
! TGATGATGAATGTGAATGTG+AGG - chr4_4:51700663-51700682 Msa0687030:intron 35.0%
GATGATGAATGTGAATGTGA+GGG - chr4_4:51700664-51700683 Msa0687030:intron 35.0%
! GTGAATGTGAGGGATAATTT+TGG - chr4_4:51700674-51700693 Msa0687030:intron 35.0%
TCTCTTATTCTTGATGCTGT+TGG + chr4_4:51700717-51700736 None:intergenic 35.0%
GATACTATACGCAACATCAA+AGG - chr4_4:51700740-51700759 Msa0687030:CDS 35.0%
TATACGCAACATCAAAGGAT+GGG - chr4_4:51700745-51700764 Msa0687030:CDS 35.0%
!! AATTAAGAGGGGCATCTATT+TGG + chr4_4:51700856-51700875 None:intergenic 35.0%
AGCTTGGCACATAATTAAGA+GGG + chr4_4:51700868-51700887 None:intergenic 35.0%
TAGCTTGGCACATAATTAAG+AGG + chr4_4:51700869-51700888 None:intergenic 35.0%
AGAACATGTCATAGAGTCAT+TGG + chr4_4:51700962-51700981 None:intergenic 35.0%
TCGGGTGTATGATAAGTTAA+GGG - chr4_4:51701111-51701130 Msa0687030:intron 35.0%
GATGAGAAGAAGAACCAAGT+TGG + chr4_4:51699860-51699879 None:intergenic 40.0%
TCATCAGATAACAATGGCGA+TGG - chr4_4:51699875-51699894 Msa0687030:three_prime_UTR 40.0%
AATGGCGATGGCTTTCAAAA+TGG - chr4_4:51699887-51699906 Msa0687030:three_prime_UTR 40.0%
AATCTGAACAGGCTACTACT+GGG - chr4_4:51700055-51700074 Msa0687030:three_prime_UTR 40.0%
ACTCTTTCATGGAGATGAAG+TGG - chr4_4:51700103-51700122 Msa0687030:three_prime_UTR 40.0%
TGAAGAAGATCTTGCTCTTG+CGG + chr4_4:51700299-51700318 None:intergenic 40.0%
GCAAGATCTTCTTCATTGCA+TGG - chr4_4:51700304-51700323 Msa0687030:intron 40.0%
CTATACGCAACATCAAAGGA+TGG - chr4_4:51700744-51700763 Msa0687030:CDS 40.0%
GCTTGGCACATAATTAAGAG+GGG + chr4_4:51700867-51700886 None:intergenic 40.0%
ATACCCCACTTACATCACAA+TGG + chr4_4:51701039-51701058 None:intergenic 40.0%
GTCGGGTGTATGATAAGTTA+AGG - chr4_4:51701110-51701129 Msa0687030:intron 40.0%
AGTTAAGGGCAACAAACCTT+TGG - chr4_4:51701125-51701144 Msa0687030:exon 40.0%
GATGAGAAGAAGAACCAAGT+TGG + chr4_4:51699860-51699879 None:intergenic 40.0%
TCATCAGATAACAATGGCGA+TGG - chr4_4:51699875-51699894 Msa0687030:three_prime_UTR 40.0%
AATGGCGATGGCTTTCAAAA+TGG - chr4_4:51699887-51699906 Msa0687030:three_prime_UTR 40.0%
AATCTGAACAGGCTACTACT+GGG - chr4_4:51700055-51700074 Msa0687030:three_prime_UTR 40.0%
ACTCTTTCATGGAGATGAAG+TGG - chr4_4:51700103-51700122 Msa0687030:three_prime_UTR 40.0%
TGAAGAAGATCTTGCTCTTG+CGG + chr4_4:51700299-51700318 None:intergenic 40.0%
GCAAGATCTTCTTCATTGCA+TGG - chr4_4:51700304-51700323 Msa0687030:intron 40.0%
CTATACGCAACATCAAAGGA+TGG - chr4_4:51700744-51700763 Msa0687030:CDS 40.0%
GCTTGGCACATAATTAAGAG+GGG + chr4_4:51700867-51700886 None:intergenic 40.0%
ATACCCCACTTACATCACAA+TGG + chr4_4:51701039-51701058 None:intergenic 40.0%
GTCGGGTGTATGATAAGTTA+AGG - chr4_4:51701110-51701129 Msa0687030:intron 40.0%
AGTTAAGGGCAACAAACCTT+TGG - chr4_4:51701125-51701144 Msa0687030:exon 40.0%
GAATCTGAACAGGCTACTAC+TGG - chr4_4:51700054-51700073 Msa0687030:three_prime_UTR 45.0%
CATGGAGATGAAGTGGAAGA+AGG - chr4_4:51700110-51700129 Msa0687030:three_prime_UTR 45.0%
! TGAAGTGGAAGAAGGTGCAT+AGG - chr4_4:51700118-51700137 Msa0687030:three_prime_UTR 45.0%
AATTGGACTCTACAAAGCCG+TGG - chr4_4:51700415-51700434 Msa0687030:intron 45.0%
!! GGATGGGTTAAGAAGAGCTT+TGG - chr4_4:51700761-51700780 Msa0687030:CDS 45.0%
!! GGGTTAAGAAGAGCTTTGGA+TGG - chr4_4:51700765-51700784 Msa0687030:CDS 45.0%
GAATCCCATCTCAGTTGGAT+CGG + chr4_4:51700814-51700833 None:intergenic 45.0%
ACATCGAATCCCATCTCAGT+TGG + chr4_4:51700819-51700838 None:intergenic 45.0%
TGGCACCATTGTGATGTAAG+TGG - chr4_4:51701031-51701050 Msa0687030:intron 45.0%
GGCACCATTGTGATGTAAGT+GGG - chr4_4:51701032-51701051 Msa0687030:intron 45.0%
GCACCATTGTGATGTAAGTG+GGG - chr4_4:51701033-51701052 Msa0687030:intron 45.0%
TGGTTGAGACTTGAGAGCAA+TGG - chr4_4:51701173-51701192 Msa0687030:five_prime_UTR 45.0%
ATTTGAAGGGAATGGGACTC+TGG + chr4_4:51699784-51699803 None:intergenic 45.0%
GAATCTGAACAGGCTACTAC+TGG - chr4_4:51700054-51700073 Msa0687030:three_prime_UTR 45.0%
CATGGAGATGAAGTGGAAGA+AGG - chr4_4:51700110-51700129 Msa0687030:three_prime_UTR 45.0%
! TGAAGTGGAAGAAGGTGCAT+AGG - chr4_4:51700118-51700137 Msa0687030:three_prime_UTR 45.0%
AATTGGACTCTACAAAGCCG+TGG - chr4_4:51700415-51700434 Msa0687030:intron 45.0%
!! GGATGGGTTAAGAAGAGCTT+TGG - chr4_4:51700761-51700780 Msa0687030:CDS 45.0%
!! GGGTTAAGAAGAGCTTTGGA+TGG - chr4_4:51700765-51700784 Msa0687030:CDS 45.0%
GAATCCCATCTCAGTTGGAT+CGG + chr4_4:51700814-51700833 None:intergenic 45.0%
ACATCGAATCCCATCTCAGT+TGG + chr4_4:51700819-51700838 None:intergenic 45.0%
TGGCACCATTGTGATGTAAG+TGG - chr4_4:51701031-51701050 Msa0687030:intron 45.0%
GGCACCATTGTGATGTAAGT+GGG - chr4_4:51701032-51701051 Msa0687030:intron 45.0%
GCACCATTGTGATGTAAGTG+GGG - chr4_4:51701033-51701052 Msa0687030:intron 45.0%
TGGTTGAGACTTGAGAGCAA+TGG - chr4_4:51701173-51701192 Msa0687030:five_prime_UTR 45.0%
!!! AATTTATGATATTTTTATAA+TGG + chr4_4:51699816-51699835 None:intergenic 5.0%
!!! AATTTATGATATTTTTATAA+TGG + chr4_4:51699816-51699835 None:intergenic 5.0%
AACAGGCTACTACTGGGATG+TGG - chr4_4:51700061-51700080 Msa0687030:three_prime_UTR 50.0%
ACAGGCTACTACTGGGATGT+GGG - chr4_4:51700062-51700081 Msa0687030:three_prime_UTR 50.0%
AAGGCTTGTGACCGTTGACA+AGG - chr4_4:51700170-51700189 Msa0687030:CDS 50.0%
CTTGTGACCGTTGACAAGGT+TGG - chr4_4:51700174-51700193 Msa0687030:CDS 50.0%
AGGACCACCAACCTTGTCAA+CGG + chr4_4:51700184-51700203 None:intergenic 50.0%
TTATATCGTCTACGGCGCCA+CGG + chr4_4:51700435-51700454 None:intergenic 50.0%
TCTCAGTTGGATCGGTTGCT+TGG + chr4_4:51700806-51700825 None:intergenic 50.0%
AGCAACCGATCCAACTGAGA+TGG - chr4_4:51700806-51700825 Msa0687030:CDS 50.0%
GCAACCGATCCAACTGAGAT+GGG - chr4_4:51700807-51700826 Msa0687030:CDS 50.0%
AACAGGCTACTACTGGGATG+TGG - chr4_4:51700061-51700080 Msa0687030:three_prime_UTR 50.0%
ACAGGCTACTACTGGGATGT+GGG - chr4_4:51700062-51700081 Msa0687030:three_prime_UTR 50.0%
AAGGCTTGTGACCGTTGACA+AGG - chr4_4:51700170-51700189 Msa0687030:CDS 50.0%
CTTGTGACCGTTGACAAGGT+TGG - chr4_4:51700174-51700193 Msa0687030:CDS 50.0%
AGGACCACCAACCTTGTCAA+CGG + chr4_4:51700184-51700203 None:intergenic 50.0%
TTATATCGTCTACGGCGCCA+CGG + chr4_4:51700435-51700454 None:intergenic 50.0%
TCTCAGTTGGATCGGTTGCT+TGG + chr4_4:51700806-51700825 None:intergenic 50.0%
AGCAACCGATCCAACTGAGA+TGG - chr4_4:51700806-51700825 Msa0687030:CDS 50.0%
GCAACCGATCCAACTGAGAT+GGG - chr4_4:51700807-51700826 Msa0687030:CDS 50.0%
GTGACCGTTGACAAGGTTGG+TGG - chr4_4:51700177-51700196 Msa0687030:CDS 55.0%
!! GTTGACAAGGTTGGTGGTCC+TGG - chr4_4:51700183-51700202 Msa0687030:CDS 55.0%
GTGACCGTTGACAAGGTTGG+TGG - chr4_4:51700177-51700196 Msa0687030:CDS 55.0%
!! GTTGACAAGGTTGGTGGTCC+TGG - chr4_4:51700183-51700202 Msa0687030:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 51699784 51701240 51699784 ID=Msa0687030;Name=Msa0687030
chr4_4 mRNA 51699784 51701240 51699784 ID=Msa0687030-mRNA-1;Parent=Msa0687030;Name=Msa0687030-mRNA-1;_AED=0.03;_eAED=0.03;_QI=104|1|1|1|1|1|3|380|143
chr4_4 exon 51699784 51700314 51699784 ID=Msa0687030-mRNA-1:exon:13460;Parent=Msa0687030-mRNA-1
chr4_4 exon 51700699 51700958 51700699 ID=Msa0687030-mRNA-1:exon:13459;Parent=Msa0687030-mRNA-1
chr4_4 exon 51701116 51701240 51701116 ID=Msa0687030-mRNA-1:exon:13458;Parent=Msa0687030-mRNA-1
chr4_4 five_prime_UTR 51701137 51701240 51701137 ID=Msa0687030-mRNA-1:five_prime_utr;Parent=Msa0687030-mRNA-1
chr4_4 CDS 51701116 51701136 51701116 ID=Msa0687030-mRNA-1:cds;Parent=Msa0687030-mRNA-1
chr4_4 CDS 51700699 51700958 51700699 ID=Msa0687030-mRNA-1:cds;Parent=Msa0687030-mRNA-1
chr4_4 CDS 51700164 51700314 51700164 ID=Msa0687030-mRNA-1:cds;Parent=Msa0687030-mRNA-1
chr4_4 three_prime_UTR 51699784 51700163 51699784 ID=Msa0687030-mRNA-1:three_prime_utr;Parent=Msa0687030-mRNA-1
Gene Sequence

>Msa0687030

ATGGCGATGGCTTTCAAAATGGCTACTACTGGGATGTGGGTAACAGATGAGTGCAAAAACTCTTTCATGGAGATGAAGTGGAAGAAGGTGCATAGGTACATAGTTTTCAAGATCGATGAAAAAACAAGGCTTGTGACCGTTGACAAGGTTGGTGGTCCTGGAGAAAACTATGATGATCTTGCTGCTTCTTTGCCTAATGATGATTGTAGATATGCTGTCTTTGATTTTGATTTTGTCACTGTTGACAACTGCCGCAAGAGCAAGATCTTCTTCATTGCATGGTCACCAACAGCATCAAGAATAAGAGAAAAGATACTATACGCAACATCAAAGGATGGGTTAAGAAGAGCTTTGGATGGAATCTCATATGAACTCCAAGCAACCGATCCAACTGAGATGGGATTCGATGTAATTCAAGACAGAGCCAAATAG
Protein sequence

>Msa0687030

MAMAFKMATTGMWVTDECKNSFMEMKWKKVHRYIVFKIDEKTRLVTVDKVGGPGENYDDLAASLPNDDCRYAVFDFDFVTVDNCRKSKIFFIAWSPTASRIREKILYATSKDGLRRALDGISYELQATDPTEMGFDVIQDRAK*