AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0562000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562000 MtrunA17_Chr4g0052621 95.992 474 6 2 1 474 1 461 0.0 929
Msa0562000 MtrunA17_Chr2g0293681 68.143 474 106 4 1 474 1 429 0.0 628
Msa0562000 MtrunA17_Chr1g0194231 63.590 390 140 1 77 464 80 469 0.0 544
Msa0562000 MtrunA17_Chr7g0270361 60.859 419 160 3 56 472 46 462 0.0 540
Msa0562000 MtrunA17_Chr5g0413411 43.552 473 226 7 24 461 56 522 3.06e-126 377
Msa0562000 MtrunA17_Chr8g0365891 46.604 427 194 7 61 461 91 509 1.54e-125 375
Msa0562000 MtrunA17_Chr6g0449961 46.348 397 195 6 78 473 45 424 2.89e-122 363
Msa0562000 MtrunA17_Chr5g0413141 40.250 400 226 6 63 459 50 439 1.22e-104 319
Msa0562000 MtrunA17_Chr8g0365901 40.863 394 220 6 77 468 64 446 3.49e-101 310
Msa0562000 MtrunA17_Chr2g0332801 40.000 380 215 7 77 453 58 427 1.18e-92 288
Msa0562000 MtrunA17_Chr1g0173451 43.380 355 182 6 73 425 71 408 4.02e-92 287
Msa0562000 MtrunA17_Chr4g0020771 43.251 363 191 7 72 430 68 419 1.94e-90 282
Msa0562000 MtrunA17_Chr2g0332971 36.031 383 233 7 78 456 28 402 6.41e-80 254
Msa0562000 MtrunA17_Chr3g0089021 41.873 363 187 8 79 435 72 416 1.87e-77 248
Msa0562000 MtrunA17_Chr7g0257701 39.296 341 156 4 72 376 59 384 2.71e-73 236
Msa0562000 MtrunA17_Chr3g0121891 35.714 406 233 10 56 456 13 395 5.87e-73 236
Msa0562000 MtrunA17_Chr2g0303941 37.371 388 224 9 71 456 21 391 8.70e-73 235
Msa0562000 MtrunA17_Chr7g0257721 45.018 271 139 4 72 341 70 331 3.27e-71 229
Msa0562000 MtrunA17_Chr4g0043661 35.342 365 209 8 78 431 23 371 3.06e-69 226
Msa0562000 MtrunA17_Chr5g0418411 36.020 397 226 12 69 458 23 398 7.66e-69 225
Msa0562000 MtrunA17_Chr1g0195221 36.649 382 214 12 71 441 28 392 2.54e-67 221
Msa0562000 MtrunA17_Chr6g0461181 33.492 421 222 10 78 456 30 434 3.52e-67 221
Msa0562000 MtrunA17_Chr4g0018151 35.606 396 227 8 69 458 69 442 3.68e-67 222
Msa0562000 MtrunA17_Chr4g0043431 36.911 382 222 11 78 456 35 400 5.08e-67 220
Msa0562000 MtrunA17_Chr8g0348981 35.754 358 210 7 77 427 26 370 8.40e-67 219
Msa0562000 MtrunA17_Chr7g0230891 38.177 351 199 11 78 425 37 372 2.83e-66 218
Msa0562000 MtrunA17_Chr7g0228411 34.080 402 231 12 77 455 39 429 3.35e-66 219
Msa0562000 MtrunA17_Chr8g0337571 35.476 389 220 11 73 456 26 388 2.12e-65 216
Msa0562000 MtrunA17_Chr2g0307811 35.754 358 217 8 77 431 24 371 2.99e-65 216
Msa0562000 MtrunA17_Chr3g0121881 35.278 360 211 9 78 431 33 376 5.56e-65 215
Msa0562000 MtrunA17_Chr2g0307831 35.227 352 216 7 77 425 24 366 1.04e-64 214
Msa0562000 MtrunA17_Chr2g0307821 35.227 352 216 7 77 425 24 366 1.04e-64 214
Msa0562000 MtrunA17_Chr8g0337581 32.648 389 232 10 73 456 26 389 7.84e-61 204
Msa0562000 MtrunA17_Chr1g0150301 32.984 382 237 9 78 456 27 392 5.09e-60 201
Msa0562000 MtrunA17_Chr2g0291841 38.272 324 180 8 110 425 23 334 2.25e-59 199
Msa0562000 MtrunA17_Chr1g0150291 32.552 384 240 9 78 458 45 412 1.67e-58 198
Msa0562000 MtrunA17_Chr3g0100091 33.419 389 193 9 78 464 30 354 2.97e-58 196
Msa0562000 MtrunA17_Chr4g0067631 33.249 394 242 9 70 453 38 420 1.16e-57 196
Msa0562000 MtrunA17_Chr5g0447901 35.276 326 181 7 77 391 24 330 1.32e-56 191
Msa0562000 MtrunA17_Chr2g0295451 34.839 310 186 6 77 378 52 353 2.90e-53 183
Msa0562000 MtrunA17_Chr1g0149241 31.771 384 212 10 78 458 28 364 6.98e-52 179
Msa0562000 MtrunA17_Chr3g0093011 38.800 250 147 4 211 456 14 261 2.09e-47 164
Msa0562000 MtrunA17_Chr8g0337511 38.618 246 144 3 210 455 4 242 2.97e-47 163
Msa0562000 MtrunA17_Chr8g0336481 35.472 265 147 6 136 397 13 256 2.23e-46 162
Msa0562000 MtrunA17_Chr8g0337521 34.926 272 149 8 73 340 26 273 2.65e-46 162
Msa0562000 MtrunA17_Chr5g0425811 32.740 281 168 6 96 372 3 266 1.53e-43 154
Msa0562000 MtrunA17_Chr8g0337601 37.959 245 144 5 213 456 3 240 2.01e-40 145
Msa0562000 MtrunA17_Chr8g0337351 43.275 171 95 2 221 389 1 171 1.20e-35 130
Msa0562000 MtrunA17_Chr8g0337551 46.094 128 69 0 213 340 7 134 6.40e-28 108
Msa0562000 MtrunA17_Chr7g0273191 37.179 156 89 3 182 335 2 150 3.10e-27 106
Msa0562000 MtrunA17_Chr4g0070281 27.206 408 225 15 59 425 61 437 7.08e-25 107
Msa0562000 MtrunA17_Chr2g0301091 27.415 383 227 14 76 425 54 418 7.76e-24 103
Msa0562000 MtrunA17_Chr1g0202641 26.361 349 210 11 80 394 557 892 8.96e-19 89.7
Msa0562000 MtrunA17_Chr1g0202641 25.926 351 209 14 80 394 57 392 4.63e-17 84.3
Msa0562000 MtrunA17_Chr5g0442221 27.532 316 188 9 80 367 46 348 1.16e-18 88.2
Msa0562000 MtrunA17_Chr3g0093021 35.714 112 67 1 77 188 26 132 5.02e-18 81.6
Msa0562000 MtrunA17_Chr7g0270821 27.554 323 187 11 80 371 45 351 1.62e-17 84.7
Msa0562000 MtrunA17_Chr1g0193961 27.066 351 206 10 80 395 45 380 1.64e-17 84.7
Msa0562000 MtrunA17_Chr8g0337541 33.333 123 72 5 73 191 26 142 1.82e-17 79.3
Msa0562000 MtrunA17_Chr3g0095221 26.074 326 200 10 69 367 39 350 2.04e-17 84.3
Msa0562000 MtrunA17_Chr7g0255311 26.282 312 185 10 80 361 65 361 3.27e-17 84.0
Msa0562000 MtrunA17_Chr8g0387391 26.498 317 178 12 80 361 46 342 4.37e-17 83.6
Msa0562000 MtrunA17_Chr1g0202651 24.355 349 217 9 80 394 57 392 4.39e-17 83.6
Msa0562000 MtrunA17_Chr3g0130251 26.087 345 206 11 47 361 49 374 5.10e-17 83.6
Msa0562000 MtrunA17_Chr2g0330221 28.723 188 118 6 73 258 20 193 5.14e-17 79.7
Msa0562000 MtrunA17_Chr8g0348491 24.633 341 215 10 83 394 60 387 1.81e-15 78.6
Msa0562000 MtrunA17_Chr2g0295241 27.492 331 185 13 68 365 31 339 3.09e-15 77.8
Msa0562000 MtrunA17_Chr4g0067811 25.234 321 195 9 80 370 42 347 1.02e-14 76.3
Msa0562000 MtrunA17_Chr1g0202621 24.432 352 207 12 80 394 50 379 1.44e-14 75.9
Msa0562000 MtrunA17_Chr7g0273181 47.126 87 43 3 358 443 1 85 2.53e-14 69.7
Msa0562000 MtrunA17_Chr7g0248451 25.484 310 190 9 80 361 57 353 4.27e-14 74.3
Msa0562000 MtrunA17_Chr8g0373171 37.209 86 54 0 210 295 6 91 5.74e-14 68.6
Msa0562000 MtrunA17_Chr7g0257711 41.935 93 51 2 341 431 1 92 2.67e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0562000 AT1G48100.1 68.683 463 143 1 1 461 1 463 0.0 662
Msa0562000 AT1G02460.1 61.637 391 145 3 77 462 88 478 1.43e-180 514
Msa0562000 AT1G56710.1 57.213 409 169 4 57 462 28 433 5.92e-170 485
Msa0562000 AT4G01890.1 55.831 403 172 3 66 463 53 454 2.73e-167 480
Msa0562000 AT5G14650.1 47.179 390 190 6 78 464 51 427 5.85e-121 360
Msa0562000 AT1G60590.1 43.614 415 201 6 78 465 123 531 4.92e-118 357
Msa0562000 AT1G10640.1 44.743 409 196 6 77 461 118 520 1.81e-116 352
Msa0562000 AT1G80170.1 42.932 382 206 6 74 452 49 421 3.41e-107 326
Msa0562000 AT3G26610.1 39.394 462 252 10 7 460 10 451 4.35e-107 326
Msa0562000 AT1G70500.1 41.414 396 219 6 77 470 66 450 1.93e-105 322
Msa0562000 AT1G23460.2 41.045 402 220 7 65 462 30 418 1.10e-99 306
Msa0562000 AT1G23460.1 41.045 402 220 7 65 462 54 442 1.88e-99 306
Msa0562000 AT3G57510.1 45.299 351 175 7 80 425 70 408 4.14e-94 291
Msa0562000 AT3G07970.1 39.173 411 232 9 38 444 33 429 8.00e-91 283
Msa0562000 AT2G41850.1 42.857 350 185 6 80 425 70 408 1.73e-88 277
Msa0562000 AT1G05660.1 38.144 388 222 8 73 458 23 394 1.58e-78 250
Msa0562000 AT1G05650.1 37.436 390 226 8 71 458 21 394 2.35e-76 244
Msa0562000 AT3G15720.3 38.718 390 206 12 78 457 23 389 1.64e-75 244
Msa0562000 AT3G15720.1 38.718 390 206 12 78 457 24 390 1.84e-75 244
Msa0562000 AT2G43890.1 36.979 384 224 11 70 451 21 388 4.14e-75 241
Msa0562000 AT4G35670.1 35.955 356 210 6 78 430 24 364 7.40e-75 241
Msa0562000 AT5G14650.2 44.737 266 135 3 78 342 51 305 4.77e-74 236
Msa0562000 AT2G43870.1 39.316 351 196 7 78 425 21 357 1.18e-73 237
Msa0562000 AT3G15720.2 38.974 390 202 13 78 457 23 386 4.26e-73 238
Msa0562000 AT2G43880.1 37.076 383 218 10 78 456 31 394 2.20e-72 234
Msa0562000 AT1G80170.2 42.804 271 145 4 74 342 49 311 5.21e-72 231
Msa0562000 AT5G44840.1 34.492 374 219 8 77 443 23 377 1.46e-71 232
Msa0562000 AT1G80140.1 35.143 350 209 6 110 456 2 336 9.25e-71 228
Msa0562000 AT2G33160.1 37.624 404 225 11 59 455 19 402 3.01e-70 236
Msa0562000 AT5G27530.1 37.222 360 196 7 77 431 52 386 5.12e-70 230
Msa0562000 AT3G59850.1 37.995 379 213 11 78 451 23 384 1.79e-69 226
Msa0562000 AT1G02790.1 37.151 358 202 8 71 425 45 382 2.73e-69 227
Msa0562000 AT3G59850.2 39.377 353 194 10 78 425 67 404 3.99e-69 227
Msa0562000 AT1G65570.1 37.500 384 218 12 78 456 31 397 1.88e-68 224
Msa0562000 AT2G43860.1 36.979 384 223 11 78 458 33 400 1.72e-67 222
Msa0562000 AT1G78400.1 35.733 389 224 10 77 457 34 404 3.63e-64 213
Msa0562000 AT1G17150.1 36.104 385 226 8 77 456 32 401 7.16e-64 212
Msa0562000 AT1G43080.1 36.695 357 204 9 77 425 30 372 3.18e-63 211
Msa0562000 AT4G13760.1 36.134 357 206 9 77 425 30 372 3.21e-63 211
Msa0562000 AT5G17200.1 34.063 411 221 12 74 458 35 421 1.27e-62 209
Msa0562000 AT1G43090.1 36.695 357 204 9 77 425 30 372 1.38e-62 210
Msa0562000 AT3G14040.1 39.943 353 194 9 78 426 71 409 1.75e-61 207
Msa0562000 AT4G18180.1 37.017 362 209 8 71 425 32 381 2.00e-61 206
Msa0562000 AT5G39910.1 31.328 399 192 10 77 457 35 369 2.09e-61 205
Msa0562000 AT3G15720.4 37.356 348 186 11 119 457 2 326 6.91e-61 204
Msa0562000 AT5G44830.1 32.840 338 197 6 109 443 8 318 9.03e-61 202
Msa0562000 AT2G43880.2 38.851 296 167 5 78 372 31 313 1.08e-60 202
Msa0562000 AT1G43100.1 36.134 357 206 9 77 425 30 372 1.71e-60 204
Msa0562000 AT3G07850.1 39.377 353 196 9 78 426 70 408 1.08e-59 202
Msa0562000 AT2G26620.1 36.056 355 207 9 77 425 30 370 1.35e-59 201
Msa0562000 AT2G40310.1 35.955 356 206 9 78 425 31 372 1.85e-59 201
Msa0562000 AT3G07820.1 36.628 344 201 8 86 425 27 357 2.48e-59 200
Msa0562000 AT3G07830.1 35.799 338 201 7 91 425 34 358 2.89e-59 200
Msa0562000 AT3G07840.1 35.260 346 208 7 83 425 26 358 3.07e-59 200
Msa0562000 AT5G48140.1 35.207 338 202 7 91 425 34 357 4.46e-59 199
Msa0562000 AT4G32380.1 33.244 373 221 8 92 455 41 394 5.12e-59 201
Msa0562000 AT2G15460.1 35.475 358 207 9 77 425 30 372 7.22e-59 199
Msa0562000 AT2G15450.1 35.196 358 208 9 77 425 30 372 1.76e-58 198
Msa0562000 AT2G15470.1 34.916 358 209 9 77 425 30 372 6.67e-57 194
Msa0562000 AT4G35670.2 35.185 270 161 3 161 430 2 257 2.45e-53 181
Msa0562000 AT4G32370.1 36.860 293 163 6 92 376 49 327 7.92e-53 181
Msa0562000 AT4G32375.1 37.037 270 148 4 161 425 13 265 1.03e-50 178
Msa0562000 AT4G32380.2 29.730 370 197 8 92 455 41 353 2.15e-46 166
Msa0562000 AT4G33440.1 28.363 342 191 12 51 361 52 370 2.17e-23 103
Msa0562000 AT3G42950.1 26.842 380 233 12 76 425 64 428 7.68e-21 95.5
Msa0562000 AT3G62110.1 26.437 348 210 11 80 396 45 377 3.52e-20 93.6
Msa0562000 AT3G62110.2 26.437 348 210 11 80 396 45 377 3.52e-20 93.6
Msa0562000 AT1G19170.1 24.627 402 235 14 66 425 75 450 6.29e-20 92.8
Msa0562000 AT5G41870.1 26.316 323 189 11 77 367 44 349 2.97e-19 90.5
Msa0562000 AT5G49215.2 26.400 375 232 12 80 425 43 402 7.93e-18 86.3
Msa0562000 AT5G49215.1 26.400 375 232 12 80 425 45 404 8.08e-18 86.3
Msa0562000 AT4G23820.1 25.868 317 191 8 80 367 43 344 1.37e-17 85.5
Msa0562000 AT3G48950.1 23.962 313 192 8 80 361 47 344 3.26e-17 84.3
Msa0562000 AT2G23900.1 25.641 312 188 10 80 361 58 355 5.44e-16 80.5
Msa0562000 AT3G61490.4 24.839 310 192 10 80 361 160 456 6.83e-15 77.4
Msa0562000 AT3G61490.5 24.839 310 192 10 80 361 62 358 7.44e-15 77.0
Msa0562000 AT3G61490.2 24.839 310 192 10 80 361 47 343 8.36e-15 77.0
Msa0562000 AT3G61490.3 24.839 310 192 10 80 361 47 343 8.36e-15 77.0
Msa0562000 AT3G61490.1 24.839 310 192 10 80 361 47 343 8.36e-15 77.0
Msa0562000 AT4G23500.1 26.537 309 176 11 83 358 73 363 2.43e-13 72.4

Find 133 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4_1:-61650840 Msa0562000:CDS
TCTACCTAATCAACCAAATT+TGG 0.144380 4_1:+61650747 None:intergenic
CATCCACATTTCTCAAATTA+TGG 0.167033 4_1:+61653440 None:intergenic
TCTTAGGATTCGTCCCTATT+TGG 0.168680 4_1:-61650640 Msa0562000:three_prime_UTR
CAGTTCCACATGATATTTAA+TGG 0.196238 4_1:-61651907 Msa0562000:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4_1:+61650835 None:intergenic
CTTGACCAAATGAGTCTTAA+TGG 0.202575 4_1:-61652797 Msa0562000:CDS
TTCATGGACTATTTATTCTT+TGG 0.220446 4_1:-61653486 Msa0562000:CDS
ATGGGAAAATACACCCAAAT+AGG 0.222592 4_1:+61650626 None:intergenic
TTAATTACCATTGCTTATTA+AGG 0.227433 4_1:+61651777 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4_1:+61650883 None:intergenic
ATATTTGTGTTAAAGATAAT+TGG 0.246059 4_1:+61653618 None:intergenic
ATGATGTATTAGTCCAAATT+TGG 0.262749 4_1:-61650760 Msa0562000:three_prime_UTR
CAGCAACTCATCGCTCACTA+TGG 0.305602 4_1:+61650607 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4_1:+61652730 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4_1:+61653301 None:intergenic
CTTAGGATTCGTCCCTATTT+GGG 0.316030 4_1:-61650639 Msa0562000:three_prime_UTR
TAGTCCATTCACATACAAAT+TGG 0.324642 4_1:+61651948 None:intergenic
AACAATAGTATTGGTCTATA+AGG 0.326820 4_1:+61651094 None:intergenic
AACACAAATATTTCTGAAAC+AGG 0.328264 4_1:-61653606 Msa0562000:five_prime_UTR
TAGTCCAAATTTGGTTGATT+AGG 0.339571 4_1:-61650751 Msa0562000:three_prime_UTR
GTTACGTCATTTGGAGCAAT+TGG 0.352640 4_1:-61653259 Msa0562000:CDS
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4_1:-61653179 Msa0562000:CDS
TATATATTATATATTACATT+AGG 0.360280 4_1:-61650721 Msa0562000:three_prime_UTR
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4_1:+61652766 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4_1:+61650808 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4_1:+61653370 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4_1:-61651633 Msa0562000:CDS
ACATGGATTCCATCAGTGTT+GGG 0.385395 4_1:+61651832 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4_1:-61650839 Msa0562000:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4_1:+61652851 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4_1:-61653296 Msa0562000:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4_1:+61651844 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4_1:+61652860 None:intergenic
TCCCATATGGATCTCATTCA+TGG 0.407523 4_1:-61653502 Msa0562000:exon
CTGTACCATTAAGACTCATT+TGG 0.407881 4_1:+61652792 None:intergenic
CATTTATACTTATTATTATG+TGG 0.415534 4_1:-61650539 Msa0562000:three_prime_UTR
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4_1:-61651572 Msa0562000:intron
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4_1:+61653214 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4_1:+61650979 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4_1:+61652839 None:intergenic
TGGGAAAATACACCCAAATA+GGG 0.426962 4_1:+61650627 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4_1:+61653100 None:intergenic
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4_1:-61652773 Msa0562000:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4_1:+61651085 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4_1:-61652862 Msa0562000:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4_1:+61653336 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4_1:+61651831 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4_1:+61653307 None:intergenic
CCCTTATCCAAATGATCCTC+GGG 0.453352 4_1:-61653308 Msa0562000:CDS
GTCCATGAATGAGATCCATA+TGG 0.453446 4_1:+61653500 None:intergenic
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4_1:-61653190 Msa0562000:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4_1:-61652736 Msa0562000:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4_1:-61651136 Msa0562000:CDS
TGAGCGATGAGTTGCTGAAT+TGG 0.456799 4_1:-61650601 Msa0562000:three_prime_UTR
ATGGTTGACAAGTAATATAA+AGG 0.458468 4_1:+61653459 None:intergenic
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4_1:-61650911 Msa0562000:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4_1:+61650838 None:intergenic
TTTCATATCTATAGATGATA+CGG 0.467936 4_1:-61651984 Msa0562000:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4_1:-61651174 Msa0562000:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4_1:+61651608 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4_1:+61653308 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4_1:-61651811 Msa0562000:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4_1:+61651245 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4_1:-61651810 Msa0562000:CDS
AATGGTACAGGAACCATTGA+AGG 0.492445 4_1:-61652779 Msa0562000:CDS
TGAAATTGAAGAAAGAAGAA+TGG 0.492537 4_1:+61653648 None:intergenic
TCAGGTGGATGGTATGCTAA+TGG 0.494463 4_1:-61652885 Msa0562000:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4_1:-61651952 Msa0562000:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4_1:-61650835 Msa0562000:CDS
AAATCATGCTTTCACAATGT+AGG 0.499150 4_1:+61650564 None:intergenic
TAACATGTACTGCTGATGTT+TGG 0.499162 4_1:+61651052 None:intergenic
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4_1:-61651279 Msa0562000:intron
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4_1:-61651175 Msa0562000:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4_1:-61653309 Msa0562000:CDS
TCCAACAATTCACCTTCAAA+TGG 0.505935 4_1:+61650910 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4_1:+61653109 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4_1:+61651881 None:intergenic
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4_1:-61651578 Msa0562000:CDS
CAAATGAGTCTTAATGGTAC+AGG 0.523809 4_1:-61652791 Msa0562000:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4_1:-61651603 Msa0562000:CDS
CAACCATAATTTGAGAAATG+TGG 0.536333 4_1:-61653443 Msa0562000:CDS
AGCAACTCATCGCTCACTAT+GGG 0.537725 4_1:+61650608 None:intergenic
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4_1:-61651579 Msa0562000:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4_1:-61651898 Msa0562000:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4_1:-61651126 Msa0562000:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4_1:+61651609 None:intergenic
TCATTTGGAGCAATTGGAGA+TGG 0.546320 4_1:-61653253 Msa0562000:CDS
CTCACTGCTGGACTTCCACA+TGG 0.546524 4_1:+61652471 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4_1:-61652760 Msa0562000:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4_1:+61651244 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4_1:-61651841 Msa0562000:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4_1:-61651627 Msa0562000:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4_1:-61651784 Msa0562000:intron
ATTTGAGAAATGTGGATGGA+AGG 0.558934 4_1:-61653435 Msa0562000:CDS
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4_1:+61653377 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4_1:-61653216 Msa0562000:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4_1:-61652896 Msa0562000:intron
CATAATTTGAGAAATGTGGA+TGG 0.572176 4_1:-61653439 Msa0562000:CDS
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4_1:-61652763 Msa0562000:CDS
TGAAGAAAGAAGAATGGCTA+AGG 0.575307 4_1:+61653654 None:intergenic
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4_1:+61652731 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4_1:+61653316 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4_1:+61651567 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4_1:-61651085 Msa0562000:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4_1:-61650818 Msa0562000:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4_1:+61651910 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4_1:-61652735 Msa0562000:intron
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4_1:-61652835 Msa0562000:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4_1:-61651613 Msa0562000:CDS
TCCATGAATGAGATCCATAT+GGG 0.606027 4_1:+61653501 None:intergenic
TCTTATGACTGTGTCACGGA+CGG 0.610881 4_1:+61651221 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4_1:-61651183 Msa0562000:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4_1:-61652875 Msa0562000:CDS
GGCAGCCATTAAATATCATG+TGG 0.622918 4_1:+61651902 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4_1:+61651833 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4_1:+61653325 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4_1:-61651626 Msa0562000:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4_1:+61653378 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4_1:+61653340 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4_1:+61650809 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4_1:+61651562 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4_1:-61652855 Msa0562000:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4_1:-61651591 Msa0562000:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4_1:-61651207 Msa0562000:CDS
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4_1:+61651217 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4_1:-61652486 Msa0562000:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4_1:-61652759 Msa0562000:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4_1:-61650944 Msa0562000:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4_1:-61651180 Msa0562000:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4_1:+61653292 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4_1:-61651188 Msa0562000:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4_1:-61650817 Msa0562000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+TGG + chr4_1:61651147-61651166 None:intergenic 10.0%
!! TCTATAAATAAATATTTACA+TGG + chr4_1:61651517-61651536 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG - chr4_1:61651774-61651793 Msa0562000:intron 10.0%
!! TTCTATATGTAATTAATATA+GGG - chr4_1:61651775-61651794 Msa0562000:intron 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG - chr4_1:61652088-61652107 Msa0562000:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG - chr4_1:61652118-61652137 Msa0562000:intron 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG - chr4_1:61652119-61652138 Msa0562000:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG + chr4_1:61652768-61652787 None:intergenic 10.0%
!! ATATTTGTGTTAAAGATAAT+TGG + chr4_1:61650577-61650596 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG - chr4_1:61651201-61651220 Msa0562000:CDS 15.0%
!!! AAAATTTTCCATTTTGTTTT+TGG - chr4_1:61651883-61651902 Msa0562000:CDS 15.0%
!! ATGTCAAAATTATATCAATT+AGG - chr4_1:61652029-61652048 Msa0562000:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG - chr4_1:61652030-61652049 Msa0562000:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG + chr4_1:61652095-61652114 None:intergenic 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG - chr4_1:61652120-61652139 Msa0562000:intron 15.0%
!!! ATTATATGTTTTGATTGTTA+TGG - chr4_1:61653512-61653531 Msa0562000:exon 15.0%
!! CATTTATACTTATTATTATG+TGG - chr4_1:61653653-61653672 Msa0562000:five_prime_UTR 15.0%
!!! ATCATAAAAGTGTAATTTTC+TGG + chr4_1:61651041-61651060 None:intergenic 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG - chr4_1:61651059-61651078 Msa0562000:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG - chr4_1:61651289-61651308 Msa0562000:intron 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG - chr4_1:61651575-61651594 Msa0562000:CDS 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG + chr4_1:61651671-61651690 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG + chr4_1:61651851-61651870 None:intergenic 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG + chr4_1:61651880-61651899 None:intergenic 20.0%
!! ACATTAGCATAAACATAAAT+CGG + chr4_1:61651919-61651938 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG + chr4_1:61652003-61652022 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG - chr4_1:61652058-61652077 Msa0562000:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG - chr4_1:61652059-61652078 Msa0562000:intron 20.0%
!! TTTCATATCTATAGATGATA+CGG - chr4_1:61652208-61652227 Msa0562000:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG + chr4_1:61652418-61652437 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG - chr4_1:61652418-61652437 Msa0562000:intron 20.0%
!! AACATAGAAACAATAAAACT+GGG + chr4_1:61652469-61652488 None:intergenic 20.0%
!! TAACATAGAAACAATAAAAC+TGG + chr4_1:61652470-61652489 None:intergenic 20.0%
!! TTACTATTGACAAAATTTAG+AGG - chr4_1:61652787-61652806 Msa0562000:CDS 20.0%
!! AAAATTTGAGAACATACAAA+TGG - chr4_1:61653056-61653075 Msa0562000:intron 20.0%
! TGAAATTGAAGAAAGAAGAA+TGG + chr4_1:61650547-61650566 None:intergenic 25.0%
!!! AACACAAATATTTCTGAAAC+AGG - chr4_1:61650586-61650605 Msa0562000:three_prime_UTR 25.0%
!!! ATTTTGTTGTAGCACAATAT+TGG - chr4_1:61650648-61650667 Msa0562000:three_prime_UTR 25.0%
!! TTCATGGACTATTTATTCTT+TGG - chr4_1:61650706-61650725 Msa0562000:three_prime_UTR 25.0%
! ATGGTTGACAAGTAATATAA+AGG + chr4_1:61650736-61650755 None:intergenic 25.0%
! AAAATTGAAATGTAATCAGG+TGG - chr4_1:61651292-61651311 Msa0562000:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG + chr4_1:61651664-61651683 None:intergenic 25.0%
! CACTAACCAAAAATAAAACT+TGG - chr4_1:61651662-61651681 Msa0562000:intron 25.0%
!! AAATAAAACTTGGCATTTTC+AGG - chr4_1:61651672-61651691 Msa0562000:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG - chr4_1:61651673-61651692 Msa0562000:intron 25.0%
! TAAACATAAATCGGTCATTA+GGG + chr4_1:61651910-61651929 None:intergenic 25.0%
! ATAAACATAAATCGGTCATT+AGG + chr4_1:61651911-61651930 None:intergenic 25.0%
! TTAATATAACATGGACTCAA+GGG + chr4_1:61651994-61652013 None:intergenic 25.0%
! TTTAATATAACATGGACTCA+AGG + chr4_1:61651995-61652014 None:intergenic 25.0%
! CTGGGTAAGTAAATTAATAT+TGG + chr4_1:61652451-61652470 None:intergenic 25.0%
!!! AACATGATTTGATTTTGAAC+AGG - chr4_1:61652538-61652557 Msa0562000:intron 25.0%
! TGAATAGAAAAATTAGGATC+TGG + chr4_1:61652762-61652781 None:intergenic 25.0%
! TTAGAGGTGAAATATGAAAT+CGG - chr4_1:61652803-61652822 Msa0562000:CDS 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG - chr4_1:61652904-61652923 Msa0562000:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG + chr4_1:61653101-61653120 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG - chr4_1:61653352-61653371 Msa0562000:CDS 25.0%
! ACCTCCAATTAATTGTTTAA+GGG - chr4_1:61653353-61653372 Msa0562000:CDS 25.0%
! ATGATGTATTAGTCCAAATT+TGG - chr4_1:61653432-61653451 Msa0562000:CDS 25.0%
!!! TTTTTTTATGTGTGCATCTT+AGG - chr4_1:61653536-61653555 Msa0562000:five_prime_UTR 25.0%
! CAAATTTTGAACTTCCCATA+TGG - chr4_1:61650677-61650696 Msa0562000:three_prime_UTR 30.0%
CAACCATAATTTGAGAAATG+TGG - chr4_1:61650749-61650768 Msa0562000:three_prime_UTR 30.0%
CATCCACATTTCTCAAATTA+TGG + chr4_1:61650755-61650774 None:intergenic 30.0%
CATAATTTGAGAAATGTGGA+TGG - chr4_1:61650753-61650772 Msa0562000:three_prime_UTR 30.0%
! ATTTTCGATGTTACGTCATT+TGG - chr4_1:61650924-61650943 Msa0562000:CDS 30.0%
CTTACTTGAAATACTAATCC+TGG + chr4_1:61651095-61651114 None:intergenic 30.0%
TATTTAATGACTGAGCTGAA+TGG + chr4_1:61651752-61651771 None:intergenic 30.0%
ATTAGGGACCAAAAACAAAA+TGG + chr4_1:61651894-61651913 None:intergenic 30.0%
TAATATAACATGGACTCAAG+GGG + chr4_1:61651993-61652012 None:intergenic 30.0%
TAGTCCATTCACATACAAAT+TGG + chr4_1:61652247-61652266 None:intergenic 30.0%
TTAAATATCATGTGGAACTG+AGG + chr4_1:61652285-61652304 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG - chr4_1:61652285-61652304 Msa0562000:intron 30.0%
ACATATTGATCATTACTCCT+TGG + chr4_1:61652314-61652333 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG - chr4_1:61652382-61652401 Msa0562000:intron 30.0%
TACAACTCCTTAATAAGCAA+TGG - chr4_1:61652408-61652427 Msa0562000:intron 30.0%
GCTGCTTGCTTATATAAAAA+TGG + chr4_1:61652508-61652527 None:intergenic 30.0%
ACTAAACTTCGTGACAAAAT+TGG + chr4_1:61652832-61652851 None:intergenic 30.0%
! ATGGACTAAGAATAAAGACA+TGG - chr4_1:61653004-61653023 Msa0562000:intron 30.0%
AACATACAAATGGAGAATGT+AGG - chr4_1:61653066-61653085 Msa0562000:intron 30.0%
CCTTTGTCAAACAATAGTAT+TGG + chr4_1:61653110-61653129 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG - chr4_1:61653107-61653126 Msa0562000:CDS 30.0%
TGTGTCCTACAAAAACATTA+AGG - chr4_1:61653167-61653186 Msa0562000:CDS 30.0%
GTGTCCTACAAAAACATTAA+GGG - chr4_1:61653168-61653187 Msa0562000:CDS 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG + chr4_1:61653175-61653194 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG + chr4_1:61653216-61653235 None:intergenic 30.0%
CATAAGCATTCCAACAAAAA+GGG + chr4_1:61653311-61653330 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG + chr4_1:61653357-61653376 None:intergenic 30.0%
CCTTCCCTTAAACAATTAAT+TGG + chr4_1:61653360-61653379 None:intergenic 30.0%
CCAATTAATTGTTTAAGGGA+AGG - chr4_1:61653357-61653376 Msa0562000:CDS 30.0%
TAGTCCAAATTTGGTTGATT+AGG - chr4_1:61653441-61653460 Msa0562000:CDS 30.0%
TCTACCTAATCAACCAAATT+TGG + chr4_1:61653448-61653467 None:intergenic 30.0%
!!! GCTGAATTGGTTCATTTTTT+TGG - chr4_1:61653604-61653623 Msa0562000:five_prime_UTR 30.0%
AAATCATGCTTTCACAATGT+AGG + chr4_1:61653631-61653650 None:intergenic 30.0%
TGAAGAAAGAAGAATGGCTA+AGG + chr4_1:61650541-61650560 None:intergenic 35.0%
TCCATGAATGAGATCCATAT+GGG + chr4_1:61650694-61650713 None:intergenic 35.0%
ATTTGAGAAATGTGGATGGA+AGG - chr4_1:61650757-61650776 Msa0562000:three_prime_UTR 35.0%
! GGAAGATACTTTCTTTTGCT+TGG + chr4_1:61650796-61650815 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG + chr4_1:61650817-61650836 None:intergenic 35.0%
TTGAAGACTTAGAAGGAACA+AGG + chr4_1:61650818-61650837 None:intergenic 35.0%
GGATCATTTGAAGACTTAGA+AGG + chr4_1:61650825-61650844 None:intergenic 35.0%
! AATACTAATCCTGGTTTGCA+TGG + chr4_1:61651086-61651105 None:intergenic 35.0%
ATTGGACGTATGTGTAAAAC+TGG - chr4_1:61651219-61651238 Msa0562000:CDS 35.0%
TTGAAATGTAATCAGGTGGA+TGG - chr4_1:61651296-61651315 Msa0562000:intron 35.0%
CTTGACCAAATGAGTCTTAA+TGG - chr4_1:61651395-61651414 Msa0562000:intron 35.0%
CTGTACCATTAAGACTCATT+TGG + chr4_1:61651403-61651422 None:intergenic 35.0%
CAAATGAGTCTTAATGGTAC+AGG - chr4_1:61651401-61651420 Msa0562000:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG + chr4_1:61651429-61651448 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG - chr4_1:61651429-61651448 Msa0562000:intron 35.0%
TCAAAAAAGTTTACCCTGTG+AGG + chr4_1:61651473-61651492 None:intergenic 35.0%
AGCTCCAATTTGTATGTGAA+TGG - chr4_1:61652240-61652259 Msa0562000:intron 35.0%
ATGATATTTAATGGCTGCCA+AGG - chr4_1:61652294-61652313 Msa0562000:intron 35.0%
! CATCTTGTGAGTTTTCAACA+TGG + chr4_1:61652380-61652399 None:intergenic 35.0%
GTTGAAAACTCACAAGATGT+TGG - chr4_1:61652381-61652400 Msa0562000:intron 35.0%
GGTGATGATTGCATTTCAAT+TGG - chr4_1:61652559-61652578 Msa0562000:intron 35.0%
! TATTTCCACATTTGAAGTCC+CGG + chr4_1:61652587-61652606 None:intergenic 35.0%
ACTTCAAATGTGGAAATAGC+TGG - chr4_1:61652589-61652608 Msa0562000:intron 35.0%
! TAAGTTTGAGACACATGCTT+GGG + chr4_1:61652950-61652969 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG + chr4_1:61652951-61652970 None:intergenic 35.0%
ATTCTCTTATGACTGTGTCA+CGG + chr4_1:61652978-61652997 None:intergenic 35.0%
GTCATAAGAGAATCAGACAA+TGG - chr4_1:61652985-61653004 Msa0562000:intron 35.0%
CTAAGAATAAAGACATGGCA+AGG - chr4_1:61653009-61653028 Msa0562000:intron 35.0%
TAACATGTACTGCTGATGTT+TGG + chr4_1:61653143-61653162 None:intergenic 35.0%
TACAAACATAACACTCTCTG+AGG - chr4_1:61653248-61653267 Msa0562000:CDS 35.0%
TCCAACAATTCACCTTCAAA+TGG + chr4_1:61653285-61653304 None:intergenic 35.0%
CCATAAGCATTCCAACAAAA+AGG + chr4_1:61653312-61653331 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG - chr4_1:61653309-61653328 Msa0562000:CDS 35.0%
TGGGAAAATACACCCAAATA+GGG + chr4_1:61653568-61653587 None:intergenic 35.0%
ATGGGAAAATACACCCAAAT+AGG + chr4_1:61653569-61653588 None:intergenic 35.0%
TCCCATATGGATCTCATTCA+TGG - chr4_1:61650690-61650709 Msa0562000:three_prime_UTR 40.0%
GTCCATGAATGAGATCCATA+TGG + chr4_1:61650695-61650714 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG + chr4_1:61650785-61650804 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG + chr4_1:61650846-61650865 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG + chr4_1:61650879-61650898 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG - chr4_1:61650883-61650902 Msa0562000:CDS 40.0%
! GTTACGTCATTTGGAGCAAT+TGG - chr4_1:61650933-61650952 Msa0562000:CDS 40.0%
! TCATTTGGAGCAATTGGAGA+TGG - chr4_1:61650939-61650958 Msa0562000:CDS 40.0%
TTCCATGCTTTCTTGAATGC+AGG + chr4_1:61650981-61651000 None:intergenic 40.0%
AGGCAGATAGCAGAAATCAA+TGG - chr4_1:61651357-61651376 Msa0562000:intron 40.0%
AATGGTACAGGAACCATTGA+AGG - chr4_1:61651413-61651432 Msa0562000:intron 40.0%
ACAGGAACCATTGAAGGAAA+TGG - chr4_1:61651419-61651438 Msa0562000:intron 40.0%
AAGGAAATGGAGATCAATGG+TGG - chr4_1:61651432-61651451 Msa0562000:intron 40.0%
AGGAAATGGAGATCAATGGT+GGG - chr4_1:61651433-61651452 Msa0562000:intron 40.0%
! CCTGACAAAGTTTTTCCATC+TGG + chr4_1:61651700-61651719 None:intergenic 40.0%
CCAGATGGAAAAACTTTGTC+AGG - chr4_1:61651697-61651716 Msa0562000:intron 40.0%
AAAACTTTGTCAGGACCATG+TGG - chr4_1:61651706-61651725 Msa0562000:intron 40.0%
!! TGAATGGTTTTACTCACTGC+TGG + chr4_1:61651736-61651755 None:intergenic 40.0%
GGCAGCCATTAAATATCATG+TGG + chr4_1:61652293-61652312 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG + chr4_1:61652340-61652359 None:intergenic 40.0%
ACATGGATTCCATCAGTGTT+GGG + chr4_1:61652363-61652382 None:intergenic 40.0%
AACATGGATTCCATCAGTGT+TGG + chr4_1:61652364-61652383 None:intergenic 40.0%
GATTGCATTTCAATTGGACC+CGG - chr4_1:61652565-61652584 Msa0562000:intron 40.0%
ATTGCATTTCAATTGGACCC+GGG - chr4_1:61652566-61652585 Msa0562000:intron 40.0%
! ATTTCCACATTTGAAGTCCC+GGG + chr4_1:61652586-61652605 None:intergenic 40.0%
GAAATAGCTGGTGTAACTTG+TGG - chr4_1:61652601-61652620 Msa0562000:intron 40.0%
ATGTAATGTACCTAATCCCG+TGG + chr4_1:61652633-61652652 None:intergenic 40.0%
CACTACCAAGTGAAACAACA+TGG - chr4_1:61652724-61652743 Msa0562000:intron 40.0%
!! GCAAACCATGTTGTTTCACT+TGG + chr4_1:61652732-61652751 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG - chr4_1:61652913-61652932 Msa0562000:intron 40.0%
AGAATAAAGACATGGCAAGG+TGG - chr4_1:61653012-61653031 Msa0562000:intron 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG - chr4_1:61653270-61653289 Msa0562000:CDS 40.0%
GCCATTTGAAGGTGAATTGT+TGG - chr4_1:61653281-61653300 Msa0562000:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG - chr4_1:61653298-61653317 Msa0562000:CDS 40.0%
TCTTAGGATTCGTCCCTATT+TGG - chr4_1:61653552-61653571 Msa0562000:five_prime_UTR 40.0%
CTTAGGATTCGTCCCTATTT+GGG - chr4_1:61653553-61653572 Msa0562000:five_prime_UTR 40.0%
ACACTAGGAGAAGAGTTGGA+AGG + chr4_1:61650855-61650874 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG + chr4_1:61650859-61650878 None:intergenic 45.0%
TAAGGGTCAGAAGGAACACT+AGG + chr4_1:61650870-61650889 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG + chr4_1:61650887-61650906 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG - chr4_1:61650884-61650903 Msa0562000:CDS 45.0%
TCCCGAGGATCATTTGGATA+AGG + chr4_1:61650888-61650907 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG - chr4_1:61650976-61650995 Msa0562000:CDS 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG - chr4_1:61651013-61651032 Msa0562000:CDS 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG - chr4_1:61651074-61651093 Msa0562000:CDS 45.0%
TCAGGTGGATGGTATGCTAA+TGG - chr4_1:61651307-61651326 Msa0562000:intron 45.0%
TGATTTCTGCTATCTGCCTC+TGG + chr4_1:61651356-61651375 None:intergenic 45.0%
TTTACCCTGTGAGGTTTGCA+GGG + chr4_1:61651464-61651483 None:intergenic 45.0%
TATAACATGGACTCAAGGGG+TGG + chr4_1:61651990-61652009 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG + chr4_1:61652351-61652370 None:intergenic 45.0%
AAACTTAGCCCCAACACTGA+TGG - chr4_1:61652351-61652370 Msa0562000:intron 45.0%
CATGGATTCCATCAGTGTTG+GGG + chr4_1:61652362-61652381 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG + chr4_1:61652974-61652993 None:intergenic 45.0%
AAAGACATGGCAAGGTGGAA+TGG - chr4_1:61653017-61653036 Msa0562000:intron 45.0%
AAGACATGGCAAGGTGGAAT+GGG - chr4_1:61653018-61653037 Msa0562000:intron 45.0%
AAAGATCACCCACAGTCTCA+GGG + chr4_1:61653386-61653405 None:intergenic 45.0%
AGCAACTCATCGCTCACTAT+GGG + chr4_1:61653587-61653606 None:intergenic 45.0%
TGAGCGATGAGTTGCTGAAT+TGG - chr4_1:61653591-61653610 Msa0562000:five_prime_UTR 45.0%
!! TATATATTATATATTACATT+AGG - chr4_1:61653471-61653490 Msa0562000:CDS 5.0%
GACGAATCCCGAGGATCATT+TGG + chr4_1:61650894-61650913 None:intergenic 50.0%
TGATCCTCGGGATTCGTCTT+CGG - chr4_1:61650896-61650915 Msa0562000:CDS 50.0%
GGTATGCTAATGGCACCAGA+TGG - chr4_1:61651317-61651336 Msa0562000:intron 50.0%
CACCAGATGGACCTGATTCA+TGG - chr4_1:61651330-61651349 Msa0562000:intron 50.0%
ATCTTCCCTGCAAACCTCAC+AGG - chr4_1:61651456-61651475 Msa0562000:intron 50.0%
TCTTCCCTGCAAACCTCACA+GGG - chr4_1:61651457-61651476 Msa0562000:intron 50.0%
GTTTACCCTGTGAGGTTTGC+AGG + chr4_1:61651465-61651484 None:intergenic 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG - chr4_1:61651682-61651701 Msa0562000:intron 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG - chr4_1:61652613-61652632 Msa0562000:intron 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG - chr4_1:61652614-61652633 Msa0562000:intron 50.0%
ATGTACCTAATCCCGTGGCT+AGG + chr4_1:61652628-61652647 None:intergenic 50.0%
GAAAGATCACCCACAGTCTC+AGG + chr4_1:61653387-61653406 None:intergenic 50.0%
CAGCAACTCATCGCTCACTA+TGG + chr4_1:61653588-61653607 None:intergenic 50.0%
GCAGCTTGTGCTGTAGAGTC+AGG - chr4_1:61651002-61651021 Msa0562000:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG + chr4_1:61651335-61651354 None:intergenic 55.0%
TGGACCTGATTCATGGCCAG+AGG - chr4_1:61651337-61651356 Msa0562000:intron 55.0%
TCTGCCTCTGGCCATGAATC+AGG + chr4_1:61651344-61651363 None:intergenic 55.0%
CTCACTGCTGGACTTCCACA+TGG + chr4_1:61651724-61651743 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG - chr4_1:61652579-61652598 Msa0562000:intron 55.0%
GAAGGTGACCCTGAGACTGT+GGG - chr4_1:61653375-61653394 Msa0562000:CDS 55.0%
CAGTCCGAAGACGAATCCCG+AGG + chr4_1:61650903-61650922 None:intergenic 60.0%
GTGGTCCTAGCCACGGGATT+AGG - chr4_1:61652620-61652639 Msa0562000:intron 60.0%
GGAAGGTGACCCTGAGACTG+TGG - chr4_1:61653374-61653393 Msa0562000:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 61650523 61653691 61650523 ID=Msa0562000;Name=Msa0562000
chr4_1 mRNA 61650523 61653691 61650523 ID=Msa0562000-mRNA-1;Parent=Msa0562000;Name=Msa0562000-mRNA-1;_AED=0.12;_eAED=0.12;_QI=173|1|1|1|1|1|6|263|474
chr4_1 exon 61650523 61651293 61650523 ID=Msa0562000-mRNA-1:exon:19429;Parent=Msa0562000-mRNA-1
chr4_1 exon 61651573 61651654 61651573 ID=Msa0562000-mRNA-1:exon:19428;Parent=Msa0562000-mRNA-1
chr4_1 exon 61651785 61651992 61651785 ID=Msa0562000-mRNA-1:exon:19427;Parent=Msa0562000-mRNA-1
chr4_1 exon 61652476 61652520 61652476 ID=Msa0562000-mRNA-1:exon:19426;Parent=Msa0562000-mRNA-1
chr4_1 exon 61652736 61652903 61652736 ID=Msa0562000-mRNA-1:exon:19425;Parent=Msa0562000-mRNA-1
chr4_1 exon 61653105 61653691 61653105 ID=Msa0562000-mRNA-1:exon:19424;Parent=Msa0562000-mRNA-1
chr4_1 five_prime_UTR 61653519 61653691 61653519 ID=Msa0562000-mRNA-1:five_prime_utr;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61653105 61653518 61653105 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61652736 61652903 61652736 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61652476 61652520 61652476 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61651785 61651992 61651785 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61651573 61651654 61651573 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 CDS 61650786 61651293 61650786 ID=Msa0562000-mRNA-1:cds;Parent=Msa0562000-mRNA-1
chr4_1 three_prime_UTR 61650523 61650785 61650523 ID=Msa0562000-mRNA-1:three_prime_utr;Parent=Msa0562000-mRNA-1
Gene Sequence

>Msa0562000

ATGGATCTCATTCATGGACTATTTATTCTTTGGATTACCTTTATATTACTTGTCAACCATAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACGTCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTCTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCAATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTAAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTTAACCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>Msa0562000

MDLIHGLFILWITFILLVNHNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSLNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN*