AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0655250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0655250 MtrunA17_Chr4g0052621 99.492 197 1 0 170 366 265 461 1.69e-139 404
Msa0655250 MtrunA17_Chr4g0052621 89.143 175 9 1 1 175 1 165 6.24e-106 318
Msa0655250 MtrunA17_Chr7g0270361 66.154 195 66 0 170 364 268 462 1.74e-91 281
Msa0655250 MtrunA17_Chr7g0270361 44.134 179 88 5 53 224 46 219 2.32e-36 137
Msa0655250 MtrunA17_Chr1g0194231 66.310 187 63 0 170 356 283 469 1.56e-88 275
Msa0655250 MtrunA17_Chr1g0194231 52.885 104 47 1 74 175 80 183 3.14e-30 120
Msa0655250 MtrunA17_Chr2g0293681 64.467 197 36 1 170 366 267 429 2.16e-82 257
Msa0655250 MtrunA17_Chr2g0293681 64.571 175 54 3 1 175 1 167 3.84e-60 199
Msa0655250 MtrunA17_Chr5g0413411 55.435 184 79 2 171 353 341 522 1.59e-62 208
Msa0655250 MtrunA17_Chr5g0413411 35.329 167 94 3 21 175 56 220 3.90e-23 100
Msa0655250 MtrunA17_Chr8g0365891 54.348 184 81 2 171 353 328 509 2.85e-62 207
Msa0655250 MtrunA17_Chr8g0365891 44.828 116 60 3 58 172 91 203 6.78e-23 99.8
Msa0655250 MtrunA17_Chr6g0449961 49.744 195 94 2 171 365 234 424 9.45e-60 198
Msa0655250 MtrunA17_Chr5g0413141 41.304 184 103 3 171 351 258 439 4.15e-44 158
Msa0655250 MtrunA17_Chr5g0413141 40.179 112 60 2 60 171 50 154 2.21e-20 92.0
Msa0655250 MtrunA17_Chr8g0365901 43.455 191 104 3 171 360 259 446 6.97e-44 157
Msa0655250 MtrunA17_Chr8g0365901 40.196 102 60 1 74 175 64 164 2.87e-19 89.0
Msa0655250 MtrunA17_Chr2g0332801 45.604 182 96 3 165 345 248 427 2.72e-43 155
Msa0655250 MtrunA17_Chr2g0332801 33.333 99 64 2 74 171 58 155 5.42e-15 75.9
Msa0655250 MtrunA17_Chr4g0020771 51.634 153 72 2 171 322 268 419 3.30e-40 147
Msa0655250 MtrunA17_Chr4g0020771 37.864 103 60 3 69 171 68 166 4.00e-13 70.1
Msa0655250 MtrunA17_Chr1g0173451 52.703 148 68 2 171 317 262 408 1.83e-39 145
Msa0655250 MtrunA17_Chr1g0173451 37.383 107 63 3 70 175 71 174 5.92e-16 79.0
Msa0655250 MtrunA17_Chr3g0093011 41.758 182 100 4 171 348 82 261 3.00e-36 132
Msa0655250 MtrunA17_Chr2g0332971 36.404 228 127 6 128 348 186 402 8.22e-36 134
Msa0655250 MtrunA17_Chr2g0332971 33.654 104 65 3 75 175 28 130 2.28e-13 70.9
Msa0655250 MtrunA17_Chr2g0303941 41.989 181 101 4 169 348 214 391 1.60e-35 133
Msa0655250 MtrunA17_Chr2g0303941 30.400 125 82 3 68 191 21 141 1.90e-11 65.1
Msa0655250 MtrunA17_Chr2g0291841 46.309 149 77 3 171 317 187 334 9.83e-33 125
Msa0655250 MtrunA17_Chr3g0089021 50.633 158 73 3 171 327 263 416 7.18e-32 124
Msa0655250 MtrunA17_Chr8g0337511 38.983 177 101 3 171 347 73 242 1.36e-31 119
Msa0655250 MtrunA17_Chr4g0018151 37.566 189 112 4 165 350 257 442 1.61e-31 123
Msa0655250 MtrunA17_Chr4g0018151 38.235 102 59 3 66 164 69 169 7.36e-16 78.6
Msa0655250 MtrunA17_Chr4g0043431 41.111 180 100 5 171 348 225 400 2.80e-31 122
Msa0655250 MtrunA17_Chr7g0230891 44.295 149 80 3 171 317 225 372 1.59e-30 120
Msa0655250 MtrunA17_Chr7g0230891 32.203 118 75 3 75 191 37 150 1.19e-11 65.5
Msa0655250 MtrunA17_Chr1g0149241 28.324 346 169 14 75 350 28 364 5.37e-30 118
Msa0655250 MtrunA17_Chr8g0348981 40.523 153 86 3 171 319 219 370 5.52e-30 119
Msa0655250 MtrunA17_Chr8g0348981 33.333 99 58 3 74 169 26 119 9.67e-14 72.0
Msa0655250 MtrunA17_Chr2g0307831 40.000 150 86 3 171 317 218 366 6.30e-30 119
Msa0655250 MtrunA17_Chr2g0307821 40.000 150 86 3 171 317 218 366 6.30e-30 119
Msa0655250 MtrunA17_Chr3g0100091 38.503 187 105 5 171 356 177 354 6.94e-30 117
Msa0655250 MtrunA17_Chr1g0195221 41.916 167 90 5 171 333 229 392 2.74e-29 117
Msa0655250 MtrunA17_Chr2g0307811 40.385 156 88 4 171 323 218 371 3.31e-29 116
Msa0655250 MtrunA17_Chr7g0228411 34.052 232 130 7 130 347 207 429 7.19e-29 116
Msa0655250 MtrunA17_Chr7g0228411 29.703 101 64 3 74 171 39 135 4.26e-11 63.9
Msa0655250 MtrunA17_Chr3g0121891 35.426 223 129 8 128 348 186 395 2.34e-28 114
Msa0655250 MtrunA17_Chr3g0121891 34.375 128 73 4 53 175 13 134 2.61e-15 76.6
Msa0655250 MtrunA17_Chr6g0461181 38.122 181 105 4 168 348 261 434 2.68e-27 112
Msa0655250 MtrunA17_Chr6g0461181 32.075 106 67 3 75 175 30 135 8.94e-14 72.4
Msa0655250 MtrunA17_Chr7g0257701 56.122 98 43 0 171 268 287 384 1.66e-26 109
Msa0655250 MtrunA17_Chr7g0257701 32.215 149 62 4 69 181 59 204 6.82e-15 75.5
Msa0655250 MtrunA17_Chr4g0043661 37.500 160 91 3 171 323 214 371 3.15e-26 108
Msa0655250 MtrunA17_Chr4g0043661 34.653 101 62 3 75 171 23 123 5.00e-13 69.7
Msa0655250 MtrunA17_Chr1g0150301 33.708 178 113 3 171 348 220 392 3.99e-26 107
Msa0655250 MtrunA17_Chr8g0337601 37.079 178 105 4 171 348 70 240 8.70e-26 103
Msa0655250 MtrunA17_Chr3g0121881 37.013 154 94 3 171 323 225 376 1.05e-25 107
Msa0655250 MtrunA17_Chr1g0150291 33.333 180 115 3 171 350 238 412 1.56e-25 106
Msa0655250 MtrunA17_Chr8g0337581 34.783 184 113 4 165 348 213 389 1.72e-25 106
Msa0655250 MtrunA17_Chr8g0337571 38.547 179 103 4 170 348 217 388 3.04e-25 105
Msa0655250 MtrunA17_Chr8g0336481 43.697 119 64 1 171 289 141 256 3.10e-25 103
Msa0655250 MtrunA17_Chr5g0418411 36.612 183 106 6 171 350 223 398 8.92e-24 101
Msa0655250 MtrunA17_Chr5g0418411 37.719 114 64 4 66 175 23 133 6.62e-17 81.6
Msa0655250 MtrunA17_Chr4g0067631 33.005 203 130 5 146 345 221 420 3.79e-23 99.8
Msa0655250 MtrunA17_Chr7g0257721 43.925 107 58 2 69 175 70 174 1.39e-21 94.4
Msa0655250 MtrunA17_Chr7g0257721 58.730 63 26 0 171 233 269 331 2.35e-14 73.2
Msa0655250 MtrunA17_Chr5g0447901 39.130 115 68 1 171 283 216 330 5.90e-21 92.4
Msa0655250 MtrunA17_Chr5g0447901 37.755 98 57 2 74 171 24 117 7.11e-12 65.9
Msa0655250 MtrunA17_Chr8g0337351 44.248 113 61 2 171 281 59 171 7.13e-21 89.0
Msa0655250 MtrunA17_Chr2g0295451 44.340 106 53 2 171 270 248 353 2.50e-18 85.5
Msa0655250 MtrunA17_Chr5g0425811 42.268 97 53 1 171 264 170 266 8.00e-18 82.4
Msa0655250 MtrunA17_Chr7g0273181 47.126 87 43 3 250 335 1 85 1.08e-14 70.1
Msa0655250 MtrunA17_Chr3g0093021 35.714 98 58 1 74 171 26 118 2.38e-14 70.5
Msa0655250 MtrunA17_Chr8g0337551 42.857 91 50 1 144 232 44 134 5.17e-14 68.9
Msa0655250 MtrunA17_Chr8g0337541 31.731 104 65 3 70 171 26 125 1.28e-13 68.2
Msa0655250 MtrunA17_Chr8g0337521 31.429 105 66 4 70 172 26 126 4.42e-13 69.3
Msa0655250 MtrunA17_Chr7g0257711 41.935 93 51 2 233 323 1 92 1.64e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0655250 AT1G48100.1 82.065 184 33 0 170 353 280 463 3.62e-105 317
Msa0655250 AT1G48100.1 51.705 176 80 3 1 171 1 176 7.29e-50 174
Msa0655250 AT1G02460.1 64.516 186 65 1 170 354 293 478 3.67e-82 259
Msa0655250 AT1G02460.1 52.381 105 47 1 74 175 88 192 1.75e-30 122
Msa0655250 AT1G56710.1 64.737 190 66 1 165 354 245 433 2.23e-81 255
Msa0655250 AT1G56710.1 41.317 167 85 6 54 217 28 184 6.91e-30 119
Msa0655250 AT4G01890.1 59.893 187 73 2 170 355 269 454 4.20e-75 240
Msa0655250 AT4G01890.1 44.444 117 61 1 63 175 53 169 2.84e-28 115
Msa0655250 AT5G14650.1 56.383 188 78 3 171 356 242 427 5.16e-65 213
Msa0655250 AT5G14650.1 36.697 109 66 2 68 175 44 150 1.17e-15 78.2
Msa0655250 AT1G60590.1 52.660 188 86 2 171 357 346 531 1.74e-61 206
Msa0655250 AT1G60590.1 41.667 108 59 3 75 181 123 227 5.75e-20 91.7
Msa0655250 AT1G10640.1 53.261 184 83 2 171 353 339 520 7.89e-59 199
Msa0655250 AT1G10640.1 42.718 103 56 2 74 175 118 218 6.36e-20 91.7
Msa0655250 AT1G80170.1 46.286 175 92 2 171 344 248 421 7.70e-47 165
Msa0655250 AT1G80170.1 44.118 102 55 2 71 171 49 149 6.94e-21 94.0
Msa0655250 AT3G26610.1 45.263 190 94 3 171 352 264 451 1.03e-46 166
Msa0655250 AT3G26610.1 33.333 171 99 6 1 171 7 162 5.90e-22 97.4
Msa0655250 AT1G23460.2 44.809 183 97 3 171 352 237 416 1.44e-43 156
Msa0655250 AT1G23460.2 33.742 163 88 4 62 208 30 188 1.06e-20 93.2
Msa0655250 AT1G23460.1 44.809 183 97 3 171 352 261 440 2.14e-43 156
Msa0655250 AT1G23460.1 33.742 163 88 4 62 208 54 212 1.37e-20 93.2
Msa0655250 AT1G70500.1 40.933 193 110 3 171 362 261 450 3.59e-43 156
Msa0655250 AT1G70500.1 42.593 108 60 2 74 181 66 171 1.59e-20 93.2
Msa0655250 AT3G57510.1 54.422 147 66 1 171 317 263 408 2.85e-41 150
Msa0655250 AT3G57510.1 40.625 96 53 2 77 171 70 162 3.92e-17 82.8
Msa0655250 AT3G07970.1 35.274 292 166 9 63 336 143 429 3.07e-40 147
Msa0655250 AT3G07970.1 33.094 139 85 5 35 171 33 165 8.67e-16 79.0
Msa0655250 AT2G41850.1 52.381 147 69 1 171 317 263 408 2.10e-39 145
Msa0655250 AT2G41850.1 38.776 98 56 2 77 173 70 164 8.89e-15 75.9
Msa0655250 AT1G05660.1 42.857 182 99 5 171 350 216 394 8.98e-36 134
Msa0655250 AT1G05660.1 35.484 124 77 1 70 193 23 143 1.91e-15 77.4
Msa0655250 AT3G14040.1 49.333 150 73 3 171 318 261 409 2.57e-35 134
Msa0655250 AT2G43890.1 43.429 175 94 5 171 343 217 388 7.83e-35 132
Msa0655250 AT2G43890.1 31.429 105 69 1 67 171 21 122 8.60e-13 69.3
Msa0655250 AT3G07850.1 48.667 150 74 3 171 318 260 408 1.92e-34 132
Msa0655250 AT2G33160.1 44.505 182 94 4 171 347 223 402 3.63e-34 133
Msa0655250 AT2G33160.1 32.203 118 67 4 56 171 19 125 3.15e-13 71.2
Msa0655250 AT1G05650.1 41.209 182 102 5 171 350 216 394 1.76e-33 128
Msa0655250 AT1G05650.1 34.615 156 92 3 68 216 21 173 6.11e-18 84.7
Msa0655250 AT1G65570.1 44.444 180 94 6 171 348 222 397 2.13e-33 128
Msa0655250 AT2G43860.1 41.989 181 100 4 171 350 224 400 3.22e-33 128
Msa0655250 AT2G43880.1 40.659 182 98 6 171 348 219 394 3.41e-32 125
Msa0655250 AT2G43880.1 32.479 117 76 2 75 191 31 144 2.31e-14 74.3
Msa0655250 AT4G35670.2 38.994 159 95 2 164 322 101 257 1.88e-31 120
Msa0655250 AT1G80140.1 35.754 179 112 3 171 348 160 336 2.69e-31 121
Msa0655250 AT3G15720.4 40.107 187 99 6 171 349 145 326 6.15e-31 121
Msa0655250 AT2G43870.1 43.243 148 82 2 171 317 211 357 6.59e-31 121
Msa0655250 AT2G43870.1 28.415 183 112 6 75 255 21 186 3.69e-11 64.3
Msa0655250 AT4G18180.1 40.994 161 90 4 160 317 223 381 6.85e-31 122
Msa0655250 AT4G35670.1 38.994 159 95 2 164 322 208 364 7.30e-31 121
Msa0655250 AT4G35670.1 36.538 104 62 3 75 175 24 126 9.94e-14 72.4
Msa0655250 AT2G40310.1 44.079 152 79 3 171 317 222 372 7.50e-31 121
Msa0655250 AT3G15720.2 40.107 187 99 6 171 349 205 386 8.43e-31 122
Msa0655250 AT3G15720.2 40.517 116 63 5 75 188 23 134 2.09e-15 77.8
Msa0655250 AT1G43080.1 44.079 152 79 3 171 317 222 372 8.99e-31 121
Msa0655250 AT3G15720.3 40.107 187 99 6 171 349 208 389 9.09e-31 122
Msa0655250 AT3G15720.3 40.517 116 63 5 75 188 23 134 2.13e-15 77.8
Msa0655250 AT3G15720.1 40.107 187 99 6 171 349 209 390 9.19e-31 122
Msa0655250 AT3G15720.1 40.517 116 63 5 75 188 24 135 2.27e-15 77.4
Msa0655250 AT1G43100.1 44.079 152 79 3 171 317 222 372 9.35e-31 122
Msa0655250 AT1G43090.1 44.079 152 79 3 171 317 222 372 1.11e-30 121
Msa0655250 AT1G17150.1 39.779 181 104 4 171 348 223 401 1.28e-30 120
Msa0655250 AT1G17150.1 35.354 99 60 1 74 172 32 126 2.65e-11 65.1
Msa0655250 AT5G48140.1 42.000 150 83 3 171 317 209 357 1.89e-30 120
Msa0655250 AT3G07820.1 40.764 157 89 3 164 317 202 357 2.51e-30 120
Msa0655250 AT3G07840.1 41.401 157 88 3 164 317 203 358 4.34e-30 119
Msa0655250 AT3G07830.1 41.333 150 84 3 171 317 210 358 6.98e-30 119
Msa0655250 AT3G59850.1 35.071 211 132 4 135 343 177 384 1.14e-29 118
Msa0655250 AT3G59850.1 29.891 184 108 8 75 255 23 188 7.23e-11 63.5
Msa0655250 AT3G59850.2 42.953 149 82 3 171 317 257 404 1.79e-29 118
Msa0655250 AT3G59850.2 29.891 184 108 8 75 255 67 232 3.98e-11 64.7
Msa0655250 AT4G13760.1 42.105 152 82 3 171 317 222 372 2.80e-29 117
Msa0655250 AT5G27530.1 41.358 162 91 3 164 323 227 386 1.03e-28 116
Msa0655250 AT5G27530.1 35.714 112 67 4 74 182 52 161 3.72e-14 73.9
Msa0655250 AT1G78400.1 39.011 182 106 4 171 349 225 404 1.13e-28 115
Msa0655250 AT1G78400.1 38.235 102 51 3 74 171 34 127 1.20e-14 75.1
Msa0655250 AT2G26620.1 41.333 150 84 3 171 317 222 370 1.73e-28 115
Msa0655250 AT2G15460.1 41.447 152 83 3 171 317 222 372 4.16e-28 114
Msa0655250 AT1G02790.1 41.290 155 85 3 164 317 233 382 5.27e-28 114
Msa0655250 AT1G02790.1 36.449 107 56 4 68 171 45 142 1.45e-11 65.9
Msa0655250 AT2G15450.1 40.789 152 84 3 171 317 222 372 1.15e-27 112
Msa0655250 AT5G44830.1 35.928 167 101 3 171 335 156 318 1.41e-27 111
Msa0655250 AT2G15470.1 40.789 152 84 3 171 317 222 372 1.71e-27 112
Msa0655250 AT4G32375.1 38.158 152 85 3 171 317 118 265 2.13e-26 109
Msa0655250 AT5G44840.1 34.731 167 103 3 171 335 215 377 4.44e-26 108
Msa0655250 AT5G44840.1 36.607 112 66 4 74 182 23 132 7.20e-15 75.9
Msa0655250 AT4G32380.1 35.556 180 111 3 171 347 217 394 4.77e-25 105
Msa0655250 AT5G17200.1 31.905 210 113 6 165 350 218 421 2.31e-22 98.2
Msa0655250 AT5G17200.1 39.252 107 60 4 71 175 35 138 2.22e-13 71.2
Msa0655250 AT1G80170.2 44.118 102 55 2 71 171 49 149 1.14e-21 94.7
Msa0655250 AT1G80170.2 51.562 64 31 0 171 234 248 311 4.70e-13 69.7
Msa0655250 AT2G43880.2 49.474 95 47 1 171 264 219 313 4.25e-21 93.2
Msa0655250 AT2G43880.2 32.479 117 76 2 75 191 31 144 1.84e-14 73.9
Msa0655250 AT5G39910.1 25.073 343 182 13 74 349 35 369 5.91e-21 93.6
Msa0655250 AT5G14650.2 64.062 64 23 0 171 234 242 305 1.56e-19 88.6
Msa0655250 AT5G14650.2 36.697 109 66 2 68 175 44 150 5.97e-16 78.2
Msa0655250 AT4G32370.1 44.954 109 56 2 164 268 219 327 1.87e-19 88.6
Msa0655250 AT4G32380.2 28.249 177 87 3 171 347 217 353 2.61e-13 71.2

Find 102 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4_3:+63800822 Msa0655250:CDS
TCTACCTAATCAACCAAATT+TGG 0.144380 4_3:-63800915 None:intergenic
TCTTAGGATTCGTCCCTATT+TGG 0.168680 4_3:+63801022 Msa0655250:three_prime_UTR
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4_3:-63800827 None:intergenic
CTTGACCAAATGAGTCTTAA+TGG 0.202575 4_3:+63798931 Msa0655250:CDS
TTCATGGACTATTTATTCTT+TGG 0.220446 4_3:+63798247 Msa0655250:CDS
ATGGGAAAATACACCCAAAT+AGG 0.222592 4_3:-63801036 None:intergenic
TCTCAAATTAAGTAGTATTA+AGG 0.230171 4_3:-63798274 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4_3:-63800779 None:intergenic
ATATTTGTGTTAAAGATAAT+TGG 0.246059 4_3:-63798115 None:intergenic
ATGATGTATTAGTCCAAATT+TGG 0.262749 4_3:+63800902 Msa0655250:three_prime_UTR
CAGCAACTCATCGCTCACTA+TGG 0.305602 4_3:-63801055 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4_3:-63798423 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4_3:+63798465 Msa0655250:CDS
CTTAGGATTCGTCCCTATTT+GGG 0.316030 4_3:+63801023 Msa0655250:three_prime_UTR
AACAATAGTATTGGTCTATA+AGG 0.326820 4_3:-63800568 None:intergenic
AACACAAATATTTCTGAAAC+AGG 0.328264 4_3:+63798127 Msa0655250:five_prime_UTR
TAGTCCAAATTTGGTTGATT+AGG 0.339571 4_3:+63800911 Msa0655250:three_prime_UTR
TGTAGACTCAGGAGTTCTTC+TGG 0.355959 4_3:+63798545 Msa0655250:CDS
TATATATTATATATTACATT+AGG 0.360280 4_3:+63800941 Msa0655250:three_prime_UTR
GAAAGATCACCCACAGTCTC+AGG 0.377658 4_3:-63800854 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4_3:-63798354 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4_3:+63800079 Msa0655250:intron
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4_3:+63800823 Msa0655250:CDS
GTGGTCCTAGCCACGGGATT+AGG 0.393113 4_3:+63800140 Msa0655250:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4_3:-63798877 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4_3:+63798428 Msa0655250:CDS
GGCCATGAATCAGGTCCATC+TGG 0.406021 4_3:-63798868 None:intergenic
TCCCATATGGATCTCATTCA+TGG 0.407523 4_3:+63798231 Msa0655250:exon
CTGTACCATTAAGACTCATT+TGG 0.407881 4_3:-63798936 None:intergenic
CATTTATACTTATTATTATG+TGG 0.415534 4_3:+63801125 Msa0655250:three_prime_UTR
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4_3:-63798510 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4_3:-63800683 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4_3:-63798889 None:intergenic
TGGGAAAATACACCCAAATA+GGG 0.426962 4_3:-63801035 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4_3:-63798624 None:intergenic
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4_3:-63800577 None:intergenic
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4_3:-63798388 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4_3:-63798417 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.453059 4_3:+63798866 Msa0655250:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4_3:+63798416 Msa0655250:CDS
GTCCATGAATGAGATCCATA+TGG 0.453446 4_3:-63798233 None:intergenic
AAAATTTGAGAACATACAAA+TGG 0.456117 4_3:+63800526 Msa0655250:CDS
TGAGCGATGAGTTGCTGAAT+TGG 0.456799 4_3:+63801061 Msa0655250:three_prime_UTR
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4_3:+63800751 Msa0655250:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4_3:-63800824 None:intergenic
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4_3:+63800488 Msa0655250:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4_3:-63800104 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4_3:-63798416 None:intergenic
TAAGTTTGAGACACATGCTT+GGG 0.483234 4_3:-63800417 None:intergenic
TGAAATTGAAGAAAGAAGAA+TGG 0.492537 4_3:-63798085 None:intergenic
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4_3:+63800827 Msa0655250:CDS
TAACATGTACTGCTGATGTT+TGG 0.499162 4_3:-63800610 None:intergenic
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4_3:+63800383 Msa0655250:intron
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4_3:+63800487 Msa0655250:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4_3:+63798415 Msa0655250:CDS
ATCATATGCTTTCACAATGT+AGG 0.502088 4_3:-63801098 None:intergenic
TCCAACAATTCACCTTCAAA+TGG 0.505935 4_3:-63800752 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4_3:-63798615 None:intergenic
ACTACTTAATTTGAGAAATG+TGG 0.520711 4_3:+63798281 Msa0655250:CDS
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4_3:+63800134 Msa0655250:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4_3:+63800109 Msa0655250:CDS
AGCAACTCATCGCTCACTAT+GGG 0.537725 4_3:-63801054 None:intergenic
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4_3:+63798471 Msa0655250:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4_3:+63800133 Msa0655250:CDS
ATTTGAGAAATGTGGATGGA+AGG 0.539093 4_3:+63798289 Msa0655250:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4_3:+63800536 Msa0655250:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4_3:-63800103 None:intergenic
TTAAGTTTGAGACACATGCT+TGG 0.553610 4_3:-63800418 None:intergenic
GATTGCATTTCAATTGGACC+CGG 0.555852 4_3:+63800085 Msa0655250:CDS
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4_3:-63798347 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4_3:+63798508 Msa0655250:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4_3:+63798832 Msa0655250:intron
CTTAATTTGAGAAATGTGGA+TGG 0.570049 4_3:+63798285 Msa0655250:CDS
GCAGCTTGTGCTGTAGACTC+AGG 0.571057 4_3:+63798534 Msa0655250:CDS
TGAAGAAAGAAGAATGGCTA+AGG 0.575307 4_3:-63798079 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4_3:-63798408 None:intergenic
ATATACCTAATCCCGTGGCT+AGG 0.594875 4_3:-63800145 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4_3:+63800577 Msa0655250:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4_3:+63800844 Msa0655250:CDS
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4_3:+63798893 Msa0655250:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4_3:+63800099 Msa0655250:CDS
TCCATGAATGAGATCCATAT+GGG 0.606027 4_3:-63798232 None:intergenic
GGTATGCTAACGGCACCAGA+TGG 0.608877 4_3:+63798853 Msa0655250:CDS
TCTTATGACTGTGTCACGGA+CGG 0.610881 4_3:-63800441 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4_3:+63800479 Msa0655250:CDS
TCAGGTGGATGGTATGCTAA+CGG 0.623376 4_3:+63798843 Msa0655250:intron
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4_3:-63798399 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4_3:+63800086 Msa0655250:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4_3:-63798346 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4_3:-63798384 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4_3:-63800853 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4_3:+63798873 Msa0655250:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4_3:+63800121 Msa0655250:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4_3:+63800455 Msa0655250:CDS
ATGTAATATACCTAATCCCG+TGG 0.656414 4_3:-63800150 None:intergenic
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4_3:-63800445 None:intergenic
TACAAACATAACACTCTCTG+AGG 0.682981 4_3:+63800718 Msa0655250:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4_3:+63800482 Msa0655250:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4_3:-63798432 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4_3:+63800474 Msa0655250:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4_3:+63800845 Msa0655250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+TGG - chr4_3:63798679-63798698 None:intergenic 10.0%
!! TCTATAAATAAATATTTACA+TGG - chr4_3:63799054-63799073 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG + chr4_3:63799311-63799330 Msa0655250:intron 10.0%
!! TTCTATATGTAATTAATATA+GGG + chr4_3:63799312-63799331 Msa0655250:intron 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG + chr4_3:63799626-63799645 Msa0655250:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG + chr4_3:63799656-63799675 Msa0655250:intron 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG + chr4_3:63799657-63799676 Msa0655250:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG - chr4_3:63800255-63800274 None:intergenic 10.0%
!! ATATTTGTGTTAAAGATAAT+TGG - chr4_3:63798118-63798137 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG + chr4_3:63798733-63798752 Msa0655250:intron 15.0%
!! ATGTCAAAATTATATCAATT+AGG + chr4_3:63799566-63799585 Msa0655250:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG + chr4_3:63799567-63799586 Msa0655250:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG - chr4_3:63799633-63799652 None:intergenic 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG + chr4_3:63799658-63799677 Msa0655250:intron 15.0%
!!! ATTATATGTTTTGATTGTTA+TGG + chr4_3:63800982-63801001 Msa0655250:three_prime_UTR 15.0%
!! CATTTATACTTATTATTATG+TGG + chr4_3:63801125-63801144 Msa0655250:three_prime_UTR 15.0%
!! TCTCAAATTAAGTAGTATTA+AGG - chr4_3:63798277-63798296 None:intergenic 20.0%
!!! ATCATAAAAGTGTAATTTTC+TGG - chr4_3:63798573-63798592 None:intergenic 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG + chr4_3:63798591-63798610 Msa0655250:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG + chr4_3:63798825-63798844 Msa0655250:intron 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG + chr4_3:63799112-63799131 Msa0655250:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG - chr4_3:63799208-63799227 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG - chr4_3:63799388-63799407 None:intergenic 20.0%
!!! AAAATTTCCCATTTTGTTTT+TGG + chr4_3:63799420-63799439 Msa0655250:intron 20.0%
!! ACATTAGCATAAACATAAAT+CGG - chr4_3:63799456-63799475 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG - chr4_3:63799540-63799559 None:intergenic 20.0%
!! TTTCATATCTATAGATGATA+CGG + chr4_3:63799743-63799762 Msa0655250:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG - chr4_3:63799953-63799972 None:intergenic 20.0%
!! TATAATAACATGATTTGAAC+AGG + chr4_3:63800058-63800077 Msa0655250:intron 20.0%
!! TTACTATTGACAAAATTTAG+AGG + chr4_3:63800274-63800293 Msa0655250:intron 20.0%
!! AAAATTTGAGAACATACAAA+TGG + chr4_3:63800526-63800545 Msa0655250:CDS 20.0%
! TGAAATTGAAGAAAGAAGAA+TGG - chr4_3:63798088-63798107 None:intergenic 25.0%
!!! AACACAAATATTTCTGAAAC+AGG + chr4_3:63798127-63798146 Msa0655250:five_prime_UTR 25.0%
!!! ATTTTGTTGTAGCACAATAT+TGG + chr4_3:63798189-63798208 Msa0655250:five_prime_UTR 25.0%
!! TTCATGGACTATTTATTCTT+TGG + chr4_3:63798247-63798266 Msa0655250:CDS 25.0%
!! ACTACTTAATTTGAGAAATG+TGG + chr4_3:63798281-63798300 Msa0655250:CDS 25.0%
!! ATTTTCGATGTTACATCATT+TGG + chr4_3:63798456-63798475 Msa0655250:CDS 25.0%
! AAAATTGAAATGTAATCAGG+TGG + chr4_3:63798828-63798847 Msa0655250:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG - chr4_3:63799201-63799220 None:intergenic 25.0%
! CACTAACCAAAAATAAAACT+TGG + chr4_3:63799199-63799218 Msa0655250:intron 25.0%
!! AAATAAAACTTGGCATTTTC+AGG + chr4_3:63799209-63799228 Msa0655250:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG + chr4_3:63799210-63799229 Msa0655250:intron 25.0%
!!! ACAAAATGGGAAATTTTGAT+AGG - chr4_3:63799417-63799436 None:intergenic 25.0%
! TAAACATAAATCGGTCATTA+GGG - chr4_3:63799447-63799466 None:intergenic 25.0%
! ATAAACATAAATCGGTCATT+AGG - chr4_3:63799448-63799467 None:intergenic 25.0%
! TTTAATATAACATGGACTCA+GGG - chr4_3:63799532-63799551 None:intergenic 25.0%
! ATTAGGGATAAAACATGAAA+CGG + chr4_3:63799583-63799602 Msa0655250:intron 25.0%
!! TAGTAACAAAGTATCAAACA+GGG - chr4_3:63800000-63800019 None:intergenic 25.0%
!! ATAGTAACAAAGTATCAAAC+AGG - chr4_3:63800001-63800020 None:intergenic 25.0%
! GCTGATTGCTTATATAAAAA+TGG - chr4_3:63800034-63800053 None:intergenic 25.0%
! TGAATAGAAAAATTAGGATC+TGG - chr4_3:63800249-63800268 None:intergenic 25.0%
! TTAGAGGTGAAATATGAAAT+CGG + chr4_3:63800290-63800309 Msa0655250:intron 25.0%
! ATTAAACTTCGTGACAAAAT+TGG - chr4_3:63800319-63800338 None:intergenic 25.0%
!! AATTTTGTCACGAAGTTTAA+TGG + chr4_3:63800318-63800337 Msa0655250:intron 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG + chr4_3:63800374-63800393 Msa0655250:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG - chr4_3:63800571-63800590 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG + chr4_3:63800822-63800841 Msa0655250:CDS 25.0%
! ACCTCCAATTAATTGTTTAA+GGG + chr4_3:63800823-63800842 Msa0655250:CDS 25.0%
! ATGATGTATTAGTCCAAATT+TGG + chr4_3:63800902-63800921 Msa0655250:three_prime_UTR 25.0%
!!! TTTTTTTATGTGTGCATCTT+AGG + chr4_3:63801006-63801025 Msa0655250:three_prime_UTR 25.0%
! CAAATTTTGAACTTCCCATA+TGG + chr4_3:63798218-63798237 Msa0655250:exon 30.0%
! CTTAATTTGAGAAATGTGGA+TGG + chr4_3:63798285-63798304 Msa0655250:CDS 30.0%
CTTACTTGAAATACTAATCC+TGG - chr4_3:63798627-63798646 None:intergenic 30.0%
TTTACATGGTGAGATATGTT+TGG - chr4_3:63799040-63799059 None:intergenic 30.0%
TATTTAATGACTGAGCTGAA+TGG - chr4_3:63799289-63799308 None:intergenic 30.0%
TTAGGGACCAAAAACAAAAT+GGG - chr4_3:63799430-63799449 None:intergenic 30.0%
ATTAGGGACCAAAAACAAAA+TGG - chr4_3:63799431-63799450 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG - chr4_3:63799531-63799550 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG - chr4_3:63799533-63799552 None:intergenic 30.0%
! ATGAAACGGAAAATTTTCGA+GGG + chr4_3:63799597-63799616 Msa0655250:intron 30.0%
TAGTCCATTCACATACAAAT+TGG - chr4_3:63799782-63799801 None:intergenic 30.0%
TTAAATATCATGTGGAACTG+AGG - chr4_3:63799820-63799839 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG + chr4_3:63799820-63799839 Msa0655250:intron 30.0%
ACATATTGATCATTACTCCT+TGG - chr4_3:63799849-63799868 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG + chr4_3:63799917-63799936 Msa0655250:intron 30.0%
TACAACTCCTTAATAAGCAA+TGG + chr4_3:63799943-63799962 Msa0655250:intron 30.0%
CAAACAGGGTAAGTAAATAT+TGG - chr4_3:63799986-63800005 None:intergenic 30.0%
! ATGGACTAAGAATAAAGACA+TGG + chr4_3:63800474-63800493 Msa0655250:CDS 30.0%
AACATACAAATGGAGAATGT+AGG + chr4_3:63800536-63800555 Msa0655250:CDS 30.0%
CCTTTGTCAAACAATAGTAT+TGG - chr4_3:63800580-63800599 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG + chr4_3:63800577-63800596 Msa0655250:CDS 30.0%
TGTGTCCTACAAAAACATTA+AGG + chr4_3:63800637-63800656 Msa0655250:CDS 30.0%
GTGTCCTACAAAAACATTAA+GGG + chr4_3:63800638-63800657 Msa0655250:CDS 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG - chr4_3:63800645-63800664 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG - chr4_3:63800686-63800705 None:intergenic 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr4_3:63800781-63800800 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG - chr4_3:63800827-63800846 None:intergenic 30.0%
CCTTCCCTTAAACAATTAAT+TGG - chr4_3:63800830-63800849 None:intergenic 30.0%
CCAATTAATTGTTTAAGGGA+AGG + chr4_3:63800827-63800846 Msa0655250:CDS 30.0%
TAGTCCAAATTTGGTTGATT+AGG + chr4_3:63800911-63800930 Msa0655250:three_prime_UTR 30.0%
TCTACCTAATCAACCAAATT+TGG - chr4_3:63800918-63800937 None:intergenic 30.0%
!!! GCTGAATTGGTTCATTTTTT+TGG + chr4_3:63801074-63801093 Msa0655250:three_prime_UTR 30.0%
ATCATATGCTTTCACAATGT+AGG - chr4_3:63801101-63801120 None:intergenic 30.0%
TGAAGAAAGAAGAATGGCTA+AGG - chr4_3:63798082-63798101 None:intergenic 35.0%
TCCATGAATGAGATCCATAT+GGG - chr4_3:63798235-63798254 None:intergenic 35.0%
ATTTGAGAAATGTGGATGGA+AGG + chr4_3:63798289-63798308 Msa0655250:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG - chr4_3:63798328-63798347 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG - chr4_3:63798349-63798368 None:intergenic 35.0%
TTGAAGACTTAGAAGGAACA+AGG - chr4_3:63798350-63798369 None:intergenic 35.0%
GGATCATTTGAAGACTTAGA+AGG - chr4_3:63798357-63798376 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG + chr4_3:63798465-63798484 Msa0655250:CDS 35.0%
! AATACTAATCCTGGTTTGCA+TGG - chr4_3:63798618-63798637 None:intergenic 35.0%
ATTGGACGTATGTGTAAAAC+TGG + chr4_3:63798751-63798770 Msa0655250:intron 35.0%
TTGAAATGTAATCAGGTGGA+TGG + chr4_3:63798832-63798851 Msa0655250:intron 35.0%
CTTGACCAAATGAGTCTTAA+TGG + chr4_3:63798931-63798950 Msa0655250:CDS 35.0%
CTGTACCATTAAGACTCATT+TGG - chr4_3:63798939-63798958 None:intergenic 35.0%
CAAATGAGTCTTAATGGTAC+AGG + chr4_3:63798937-63798956 Msa0655250:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG - chr4_3:63798965-63798984 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG + chr4_3:63798965-63798984 Msa0655250:intron 35.0%
TCAAAAAAGTTTACCCTGTG+AGG - chr4_3:63799009-63799028 None:intergenic 35.0%
TAATATAACATGGACTCAGG+GGG - chr4_3:63799530-63799549 None:intergenic 35.0%
! CATGAAACGGAAAATTTTCG+AGG + chr4_3:63799596-63799615 Msa0655250:intron 35.0%
AGCTCCAATTTGTATGTGAA+TGG + chr4_3:63799775-63799794 Msa0655250:intron 35.0%
ATGATATTTAATGGCTGCCA+AGG + chr4_3:63799829-63799848 Msa0655250:intron 35.0%
! CATCTTGTGAGTTTTCAACA+TGG - chr4_3:63799915-63799934 None:intergenic 35.0%
GTTGAAAACTCACAAGATGT+TGG + chr4_3:63799916-63799935 Msa0655250:intron 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr4_3:63800079-63800098 Msa0655250:intron 35.0%
! TATTTCCACATTTGAAGTCC+CGG - chr4_3:63800107-63800126 None:intergenic 35.0%
ACTTCAAATGTGGAAATAGC+TGG + chr4_3:63800109-63800128 Msa0655250:CDS 35.0%
ATGTAATATACCTAATCCCG+TGG - chr4_3:63800153-63800172 None:intergenic 35.0%
! TAAGTTTGAGACACATGCTT+GGG - chr4_3:63800420-63800439 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG - chr4_3:63800421-63800440 None:intergenic 35.0%
ATTCTCTTATGACTGTGTCA+CGG - chr4_3:63800448-63800467 None:intergenic 35.0%
GTCATAAGAGAATCAGACAA+TGG + chr4_3:63800455-63800474 Msa0655250:CDS 35.0%
CTAAGAATAAAGACATGGCA+AGG + chr4_3:63800479-63800498 Msa0655250:CDS 35.0%
TAACATGTACTGCTGATGTT+TGG - chr4_3:63800613-63800632 None:intergenic 35.0%
TACAAACATAACACTCTCTG+AGG + chr4_3:63800718-63800737 Msa0655250:CDS 35.0%
TCCAACAATTCACCTTCAAA+TGG - chr4_3:63800755-63800774 None:intergenic 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr4_3:63800782-63800801 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr4_3:63800779-63800798 Msa0655250:CDS 35.0%
TGGGAAAATACACCCAAATA+GGG - chr4_3:63801038-63801057 None:intergenic 35.0%
ATGGGAAAATACACCCAAAT+AGG - chr4_3:63801039-63801058 None:intergenic 35.0%
TCCCATATGGATCTCATTCA+TGG + chr4_3:63798231-63798250 Msa0655250:exon 40.0%
GTCCATGAATGAGATCCATA+TGG - chr4_3:63798236-63798255 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG - chr4_3:63798317-63798336 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG - chr4_3:63798378-63798397 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG - chr4_3:63798411-63798430 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG + chr4_3:63798415-63798434 Msa0655250:CDS 40.0%
! TCATTTGGAGCAATTGGAGA+TGG + chr4_3:63798471-63798490 Msa0655250:CDS 40.0%
TTCCATGCTTTCTTGAATGC+AGG - chr4_3:63798513-63798532 None:intergenic 40.0%
AGGCAGATAGCAGAAATCAA+TGG + chr4_3:63798893-63798912 Msa0655250:CDS 40.0%
AATGGTACAGGAACCATTGA+AGG + chr4_3:63798949-63798968 Msa0655250:intron 40.0%
ACAGGAACCATTGAAGGAAA+TGG + chr4_3:63798955-63798974 Msa0655250:intron 40.0%
AAGGAAATGGAGATCAATGG+TGG + chr4_3:63798968-63798987 Msa0655250:intron 40.0%
AGGAAATGGAGATCAATGGT+GGG + chr4_3:63798969-63798988 Msa0655250:intron 40.0%
! CCTGACAAAGTTTTTCCATC+TGG - chr4_3:63799237-63799256 None:intergenic 40.0%
CCAGATGGAAAAACTTTGTC+AGG + chr4_3:63799234-63799253 Msa0655250:intron 40.0%
AAAACTTTGTCAGGACCATG+TGG + chr4_3:63799243-63799262 Msa0655250:intron 40.0%
!! TGAATGGTTTTACTCACAGC+TGG - chr4_3:63799273-63799292 None:intergenic 40.0%
GGCAGCCATTAAATATCATG+TGG - chr4_3:63799828-63799847 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG - chr4_3:63799875-63799894 None:intergenic 40.0%
ACATGGATTCCATCAGTGTT+GGG - chr4_3:63799898-63799917 None:intergenic 40.0%
AACATGGATTCCATCAGTGT+TGG - chr4_3:63799899-63799918 None:intergenic 40.0%
GATTGCATTTCAATTGGACC+CGG + chr4_3:63800085-63800104 Msa0655250:CDS 40.0%
ATTGCATTTCAATTGGACCC+GGG + chr4_3:63800086-63800105 Msa0655250:CDS 40.0%
! ATTTCCACATTTGAAGTCCC+GGG - chr4_3:63800106-63800125 None:intergenic 40.0%
GAAATAGCTGGTGTAACTTG+TGG + chr4_3:63800121-63800140 Msa0655250:CDS 40.0%
TTGTACAGCATAGGAAGTCT+TGG + chr4_3:63800383-63800402 Msa0655250:intron 40.0%
AGAATAAAGACATGGCAAGG+TGG + chr4_3:63800482-63800501 Msa0655250:CDS 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG + chr4_3:63800740-63800759 Msa0655250:CDS 40.0%
GCCATTTGAAGGTGAATTGT+TGG + chr4_3:63800751-63800770 Msa0655250:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG + chr4_3:63800768-63800787 Msa0655250:CDS 40.0%
TCTTAGGATTCGTCCCTATT+TGG + chr4_3:63801022-63801041 Msa0655250:three_prime_UTR 40.0%
CTTAGGATTCGTCCCTATTT+GGG + chr4_3:63801023-63801042 Msa0655250:three_prime_UTR 40.0%
ACACTAGGAGAAGAGTTGGA+AGG - chr4_3:63798387-63798406 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG - chr4_3:63798391-63798410 None:intergenic 45.0%
TAAGGGTCAGAAGGAACACT+AGG - chr4_3:63798402-63798421 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG - chr4_3:63798419-63798438 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG + chr4_3:63798416-63798435 Msa0655250:CDS 45.0%
TCCCGAGGATCATTTGGATA+AGG - chr4_3:63798420-63798439 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG + chr4_3:63798508-63798527 Msa0655250:CDS 45.0%
TGTAGACTCAGGAGTTCTTC+TGG + chr4_3:63798545-63798564 Msa0655250:CDS 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG + chr4_3:63798606-63798625 Msa0655250:CDS 45.0%
TCAGGTGGATGGTATGCTAA+CGG + chr4_3:63798843-63798862 Msa0655250:intron 45.0%
TGATTTCTGCTATCTGCCTC+TGG - chr4_3:63798892-63798911 None:intergenic 45.0%
TTTACCCTGTGAGGTTTGCA+GGG - chr4_3:63799000-63799019 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG - chr4_3:63799886-63799905 None:intergenic 45.0%
AAACTTAGCCCCAACACTGA+TGG + chr4_3:63799886-63799905 Msa0655250:intron 45.0%
CATGGATTCCATCAGTGTTG+GGG - chr4_3:63799897-63799916 None:intergenic 45.0%
ATATACCTAATCCCGTGGCT+AGG - chr4_3:63800148-63800167 None:intergenic 45.0%
CACTACCAAGTGAAACAGCA+TGG + chr4_3:63800211-63800230 Msa0655250:intron 45.0%
!! GCAAACCATGCTGTTTCACT+TGG - chr4_3:63800219-63800238 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG - chr4_3:63800444-63800463 None:intergenic 45.0%
AAAGACATGGCAAGGTGGAA+TGG + chr4_3:63800487-63800506 Msa0655250:CDS 45.0%
AAGACATGGCAAGGTGGAAT+GGG + chr4_3:63800488-63800507 Msa0655250:CDS 45.0%
AAAGATCACCCACAGTCTCA+GGG - chr4_3:63800856-63800875 None:intergenic 45.0%
AGCAACTCATCGCTCACTAT+GGG - chr4_3:63801057-63801076 None:intergenic 45.0%
TGAGCGATGAGTTGCTGAAT+TGG + chr4_3:63801061-63801080 Msa0655250:three_prime_UTR 45.0%
!! TATATATTATATATTACATT+AGG + chr4_3:63800941-63800960 Msa0655250:three_prime_UTR 5.0%
GACGAATCCCGAGGATCATT+TGG - chr4_3:63798426-63798445 None:intergenic 50.0%
TGATCCTCGGGATTCGTCTT+CGG + chr4_3:63798428-63798447 Msa0655250:CDS 50.0%
CACCAGATGGACCTGATTCA+TGG + chr4_3:63798866-63798885 Msa0655250:CDS 50.0%
TTACCCTGTGAGGTTTGCAG+GGG - chr4_3:63798999-63799018 None:intergenic 50.0%
GTTTACCCTGTGAGGTTTGC+AGG - chr4_3:63799001-63799020 None:intergenic 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG + chr4_3:63799219-63799238 Msa0655250:intron 50.0%
TATAACATGGACTCAGGGGG+TGG - chr4_3:63799527-63799546 None:intergenic 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG + chr4_3:63800133-63800152 Msa0655250:CDS 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG + chr4_3:63800134-63800153 Msa0655250:CDS 50.0%
GAAAGATCACCCACAGTCTC+AGG - chr4_3:63800857-63800876 None:intergenic 50.0%
CAGCAACTCATCGCTCACTA+TGG - chr4_3:63801058-63801077 None:intergenic 50.0%
GCAGCTTGTGCTGTAGACTC+AGG + chr4_3:63798534-63798553 Msa0655250:CDS 55.0%
GGTATGCTAACGGCACCAGA+TGG + chr4_3:63798853-63798872 Msa0655250:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG - chr4_3:63798871-63798890 None:intergenic 55.0%
TGGACCTGATTCATGGCCAG+AGG + chr4_3:63798873-63798892 Msa0655250:CDS 55.0%
TCTGCCTCTGGCCATGAATC+AGG - chr4_3:63798880-63798899 None:intergenic 55.0%
ATCTCCCCTGCAAACCTCAC+AGG + chr4_3:63798992-63799011 Msa0655250:intron 55.0%
TCTCCCCTGCAAACCTCACA+GGG + chr4_3:63798993-63799012 Msa0655250:intron 55.0%
CTCACAGCTGGACTTCCACA+TGG - chr4_3:63799261-63799280 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG + chr4_3:63800099-63800118 Msa0655250:CDS 55.0%
GAAGGTGACCCTGAGACTGT+GGG + chr4_3:63800845-63800864 Msa0655250:CDS 55.0%
CAGTCCGAAGACGAATCCCG+AGG - chr4_3:63798435-63798454 None:intergenic 60.0%
GTGGTCCTAGCCACGGGATT+AGG + chr4_3:63800140-63800159 Msa0655250:CDS 60.0%
GGAAGGTGACCCTGAGACTG+TGG + chr4_3:63800844-63800863 Msa0655250:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 63798064 63801163 63798064 ID=Msa0655250;Name=Msa0655250
chr4_3 mRNA 63798064 63801163 63798064 ID=Msa0655250-mRNA-1;Parent=Msa0655250;Name=Msa0655250-mRNA-1;_AED=0.19;_eAED=0.19;_QI=173|0.66|0.75|1|1|1|4|265|366
chr4_3 exon 63798064 63798641 63798064 ID=Msa0655250-mRNA-1:exon:17966;Parent=Msa0655250-mRNA-1
chr4_3 exon 63798847 63798952 63798847 ID=Msa0655250-mRNA-1:exon:17967;Parent=Msa0655250-mRNA-1
chr4_3 exon 63800080 63800161 63800080 ID=Msa0655250-mRNA-1:exon:17968;Parent=Msa0655250-mRNA-1
chr4_3 exon 63800391 63801163 63800391 ID=Msa0655250-mRNA-1:exon:17969;Parent=Msa0655250-mRNA-1
chr4_3 five_prime_UTR 63798064 63798236 63798064 ID=Msa0655250-mRNA-1:five_prime_utr;Parent=Msa0655250-mRNA-1
chr4_3 CDS 63798237 63798641 63798237 ID=Msa0655250-mRNA-1:cds;Parent=Msa0655250-mRNA-1
chr4_3 CDS 63798847 63798952 63798847 ID=Msa0655250-mRNA-1:cds;Parent=Msa0655250-mRNA-1
chr4_3 CDS 63800080 63800161 63800080 ID=Msa0655250-mRNA-1:cds;Parent=Msa0655250-mRNA-1
chr4_3 CDS 63800391 63800898 63800391 ID=Msa0655250-mRNA-1:cds;Parent=Msa0655250-mRNA-1
chr4_3 three_prime_UTR 63800899 63801163 63800899 ID=Msa0655250-mRNA-1:three_prime_utr;Parent=Msa0655250-mRNA-1
Gene Sequence

>Msa0655250

ATGGATCTCATTCATGGACTATTTATTCTTTGGATTACCTTAATACTACTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGACTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTGGATGGTATGCTAACGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTCTTAATGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTTAACCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>Msa0655250

MDLIHGLFILWITLILLNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVDSGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLTAPDGPDSWPEADSRNQWLVFYKLDQMSLNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN*