AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0615920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615920 MtrunA17_Chr4g0052621 95.966 471 9 1 1 471 1 461 0.0 931
Msa0615920 MtrunA17_Chr2g0293681 68.365 471 107 4 1 471 1 429 0.0 628
Msa0615920 MtrunA17_Chr1g0194231 63.333 390 141 1 74 461 80 469 0.0 542
Msa0615920 MtrunA17_Chr7g0270361 60.621 419 161 3 53 469 46 462 0.0 538
Msa0615920 MtrunA17_Chr5g0413411 43.552 473 226 7 21 458 56 522 7.80e-126 376
Msa0615920 MtrunA17_Chr8g0365891 46.604 427 194 7 58 458 91 509 4.14e-125 374
Msa0615920 MtrunA17_Chr6g0449961 46.348 397 195 6 75 470 45 424 7.01e-122 362
Msa0615920 MtrunA17_Chr5g0413141 40.250 400 226 6 60 456 50 439 2.33e-104 318
Msa0615920 MtrunA17_Chr8g0365901 41.117 394 219 6 74 465 64 446 4.66e-102 313
Msa0615920 MtrunA17_Chr2g0332801 40.106 379 214 7 75 450 59 427 2.87e-92 287
Msa0615920 MtrunA17_Chr1g0173451 43.333 360 184 7 70 427 71 412 6.28e-92 286
Msa0615920 MtrunA17_Chr4g0020771 43.251 363 191 7 69 427 68 419 6.71e-90 281
Msa0615920 MtrunA17_Chr2g0332971 36.031 383 233 7 75 453 28 402 1.14e-79 253
Msa0615920 MtrunA17_Chr3g0089021 41.873 363 187 8 76 432 72 416 2.57e-77 248
Msa0615920 MtrunA17_Chr7g0257701 39.296 341 156 4 69 373 59 384 6.18e-73 236
Msa0615920 MtrunA17_Chr3g0121891 35.714 406 233 10 53 453 13 395 8.45e-73 235
Msa0615920 MtrunA17_Chr2g0303941 37.371 388 224 9 68 453 21 391 1.31e-72 234
Msa0615920 MtrunA17_Chr7g0257721 45.018 271 139 4 69 338 70 331 5.14e-71 228
Msa0615920 MtrunA17_Chr4g0043661 35.342 365 209 8 75 428 23 371 5.23e-69 225
Msa0615920 MtrunA17_Chr5g0418411 36.020 397 226 12 66 455 23 398 1.49e-68 224
Msa0615920 MtrunA17_Chr4g0018151 35.606 396 227 8 66 455 69 442 3.74e-67 222
Msa0615920 MtrunA17_Chr6g0461181 33.492 421 222 10 75 453 30 434 4.66e-67 221
Msa0615920 MtrunA17_Chr4g0043431 36.911 382 222 11 75 453 35 400 1.10e-66 219
Msa0615920 MtrunA17_Chr1g0195221 36.387 382 215 12 68 438 28 392 1.14e-66 219
Msa0615920 MtrunA17_Chr8g0348981 35.754 358 210 7 74 424 26 370 1.74e-66 219
Msa0615920 MtrunA17_Chr7g0230891 38.177 351 199 11 75 422 37 372 3.75e-66 218
Msa0615920 MtrunA17_Chr7g0228411 34.080 402 231 12 74 452 39 429 6.10e-66 218
Msa0615920 MtrunA17_Chr8g0337571 35.476 389 220 11 70 453 26 388 3.78e-65 215
Msa0615920 MtrunA17_Chr3g0121881 35.278 360 211 9 75 428 33 376 8.65e-65 214
Msa0615920 MtrunA17_Chr2g0307811 35.475 358 218 8 74 428 24 371 9.65e-65 214
Msa0615920 MtrunA17_Chr2g0307831 34.943 352 217 7 74 422 24 366 2.33e-64 214
Msa0615920 MtrunA17_Chr2g0307821 34.943 352 217 7 74 422 24 366 2.33e-64 214
Msa0615920 MtrunA17_Chr8g0337581 32.648 389 232 10 70 453 26 389 1.21e-60 203
Msa0615920 MtrunA17_Chr1g0150301 32.723 382 238 9 75 453 27 392 1.92e-59 200
Msa0615920 MtrunA17_Chr2g0291841 38.272 324 180 8 107 422 23 334 2.90e-59 199
Msa0615920 MtrunA17_Chr1g0150291 32.292 384 241 9 75 455 45 412 3.72e-58 197
Msa0615920 MtrunA17_Chr3g0100091 33.419 389 193 9 75 461 30 354 4.54e-58 195
Msa0615920 MtrunA17_Chr4g0067631 33.249 394 242 9 67 450 38 420 3.01e-57 195
Msa0615920 MtrunA17_Chr5g0447901 34.969 326 182 7 74 388 24 330 4.39e-56 189
Msa0615920 MtrunA17_Chr2g0295451 34.516 310 187 6 74 375 52 353 1.00e-52 182
Msa0615920 MtrunA17_Chr1g0149241 31.771 384 212 10 75 455 28 364 9.61e-52 179
Msa0615920 MtrunA17_Chr3g0093011 38.800 250 147 4 208 453 14 261 2.33e-47 164
Msa0615920 MtrunA17_Chr8g0337511 38.618 246 144 3 207 452 4 242 2.98e-47 163
Msa0615920 MtrunA17_Chr8g0337521 34.926 272 149 8 70 337 26 273 3.79e-46 162
Msa0615920 MtrunA17_Chr8g0336481 35.472 265 147 6 133 394 13 256 4.38e-46 161
Msa0615920 MtrunA17_Chr5g0425811 32.740 281 168 6 93 369 3 266 2.68e-43 154
Msa0615920 MtrunA17_Chr8g0337601 37.959 245 144 5 210 453 3 240 2.67e-40 145
Msa0615920 MtrunA17_Chr8g0337351 43.275 171 95 2 218 386 1 171 1.26e-35 130
Msa0615920 MtrunA17_Chr8g0337551 46.094 128 69 0 210 337 7 134 6.55e-28 108
Msa0615920 MtrunA17_Chr7g0273191 37.179 156 89 3 179 332 2 150 2.99e-27 106
Msa0615920 MtrunA17_Chr4g0070281 27.206 408 225 15 56 422 61 437 1.16e-24 106
Msa0615920 MtrunA17_Chr2g0301091 27.415 383 227 14 73 422 54 418 9.09e-24 103
Msa0615920 MtrunA17_Chr1g0202641 26.074 349 211 10 77 391 557 892 1.43e-18 89.0
Msa0615920 MtrunA17_Chr1g0202641 25.926 351 209 14 77 391 57 392 7.57e-17 83.6
Msa0615920 MtrunA17_Chr5g0442221 27.532 316 188 9 77 364 46 348 2.27e-18 87.4
Msa0615920 MtrunA17_Chr3g0093021 35.714 112 67 1 74 185 26 132 1.04e-17 80.9
Msa0615920 MtrunA17_Chr1g0193961 27.066 351 206 10 77 392 45 380 1.99e-17 84.7
Msa0615920 MtrunA17_Chr3g0095221 26.074 326 200 10 66 364 39 350 2.10e-17 84.3
Msa0615920 MtrunA17_Chr7g0270821 27.245 323 188 10 77 368 45 351 2.21e-17 84.3
Msa0615920 MtrunA17_Chr8g0337541 33.333 123 72 5 70 188 26 142 2.79e-17 79.0
Msa0615920 MtrunA17_Chr7g0255311 26.282 312 185 10 77 358 65 361 4.77e-17 83.6
Msa0615920 MtrunA17_Chr3g0130251 26.087 345 206 11 44 358 49 374 5.63e-17 83.2
Msa0615920 MtrunA17_Chr8g0387391 26.498 317 178 12 77 358 46 342 5.72e-17 83.2
Msa0615920 MtrunA17_Chr1g0202651 24.355 349 217 9 77 391 57 392 6.01e-17 83.2
Msa0615920 MtrunA17_Chr2g0330221 28.191 188 119 6 70 255 20 193 2.15e-16 77.8
Msa0615920 MtrunA17_Chr8g0348491 24.633 341 215 10 80 391 60 387 2.84e-15 77.8
Msa0615920 MtrunA17_Chr2g0295241 27.492 331 185 13 65 362 31 339 5.44e-15 77.0
Msa0615920 MtrunA17_Chr4g0067811 25.234 321 195 9 77 367 42 347 1.39e-14 75.9
Msa0615920 MtrunA17_Chr1g0202621 24.432 352 207 12 77 391 50 379 2.06e-14 75.1
Msa0615920 MtrunA17_Chr7g0273181 47.126 87 43 3 355 440 1 85 2.48e-14 69.7
Msa0615920 MtrunA17_Chr7g0248451 26.045 311 187 10 77 358 57 353 5.21e-14 73.9
Msa0615920 MtrunA17_Chr8g0373171 37.209 86 54 0 207 292 6 91 5.53e-14 68.6
Msa0615920 MtrunA17_Chr7g0257711 41.935 93 51 2 338 428 1 92 2.73e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0615920 AT1G48100.1 69.114 463 138 3 1 458 1 463 0.0 659
Msa0615920 AT1G02460.1 61.381 391 146 3 74 459 88 478 6.87e-180 513
Msa0615920 AT1G56710.1 57.213 409 169 4 54 459 28 433 1.63e-169 484
Msa0615920 AT4G01890.1 55.583 403 173 3 63 460 53 454 9.98e-167 478
Msa0615920 AT5G14650.1 47.179 390 190 6 75 461 51 427 1.09e-120 360
Msa0615920 AT1G60590.1 43.614 415 201 6 75 462 123 531 9.68e-118 356
Msa0615920 AT1G10640.1 44.743 409 196 6 74 458 118 520 5.19e-116 351
Msa0615920 AT1G80170.1 42.932 382 206 6 71 449 49 421 6.05e-107 325
Msa0615920 AT3G26610.1 40.225 445 238 10 21 457 27 451 9.46e-107 325
Msa0615920 AT1G70500.1 41.667 396 218 6 74 467 66 450 2.43e-106 324
Msa0615920 AT1G23460.2 41.294 402 219 7 62 459 30 418 1.35e-100 308
Msa0615920 AT1G23460.1 41.294 402 219 7 62 459 54 442 2.21e-100 308
Msa0615920 AT3G57510.1 45.299 351 175 7 77 422 70 408 7.12e-94 291
Msa0615920 AT3G07970.1 39.173 411 232 9 35 441 33 429 8.05e-91 283
Msa0615920 AT2G41850.1 42.857 350 185 6 77 422 70 408 2.85e-88 276
Msa0615920 AT1G05660.1 37.887 388 223 8 70 455 23 394 5.94e-78 248
Msa0615920 AT1G05650.1 37.179 390 227 8 68 455 21 394 9.95e-76 243
Msa0615920 AT3G15720.1 38.718 390 206 12 75 454 24 390 3.07e-75 243
Msa0615920 AT3G15720.3 38.718 390 206 12 75 454 23 389 3.18e-75 243
Msa0615920 AT2G43890.1 36.979 384 224 11 67 448 21 388 1.02e-74 240
Msa0615920 AT4G35670.1 35.955 356 210 6 75 427 24 364 1.88e-74 239
Msa0615920 AT5G14650.2 44.737 266 135 3 75 339 51 305 8.00e-74 235
Msa0615920 AT2G43870.1 39.316 351 196 7 75 422 21 357 2.00e-73 236
Msa0615920 AT3G15720.2 38.918 388 205 12 75 454 23 386 1.06e-72 237
Msa0615920 AT2G43880.1 37.076 383 218 10 75 453 31 394 5.89e-72 233
Msa0615920 AT1G80170.2 42.804 271 145 4 71 339 49 311 6.90e-72 231
Msa0615920 AT5G44840.1 34.492 374 219 8 74 440 23 377 2.10e-71 231
Msa0615920 AT1G80140.1 35.143 350 209 6 107 453 2 336 1.73e-70 227
Msa0615920 AT2G33160.1 37.624 404 225 11 56 452 19 402 7.28e-70 234
Msa0615920 AT5G27530.1 37.222 360 196 7 74 428 52 386 8.02e-70 229
Msa0615920 AT3G59850.1 37.995 379 213 11 75 448 23 384 3.31e-69 226
Msa0615920 AT1G02790.1 36.872 358 203 8 68 422 45 382 7.85e-69 226
Msa0615920 AT3G59850.2 39.377 353 194 10 75 422 67 404 8.43e-69 226
Msa0615920 AT1G65570.1 37.500 384 218 12 75 453 31 397 3.19e-68 223
Msa0615920 AT2G43860.1 36.979 384 223 11 75 455 33 400 2.26e-67 221
Msa0615920 AT1G17150.1 36.104 385 226 8 74 453 32 401 1.04e-63 212
Msa0615920 AT1G78400.1 35.733 389 224 10 74 454 34 404 1.05e-63 212
Msa0615920 AT4G13760.1 36.134 357 206 9 74 422 30 372 4.59e-63 210
Msa0615920 AT1G43080.1 36.695 357 204 9 74 422 30 372 6.71e-63 209
Msa0615920 AT5G17200.1 34.063 411 221 12 71 455 35 421 1.79e-62 209
Msa0615920 AT1G43090.1 36.695 357 204 9 74 422 30 372 3.13e-62 209
Msa0615920 AT5G39910.1 31.328 399 192 10 74 454 35 369 3.70e-61 204
Msa0615920 AT3G14040.1 39.660 353 195 9 75 423 71 409 4.70e-61 206
Msa0615920 AT4G18180.1 36.413 368 203 8 68 422 32 381 6.56e-61 205
Msa0615920 AT2G43880.2 38.851 296 167 5 75 369 31 313 1.68e-60 201
Msa0615920 AT5G44830.1 32.840 338 197 6 106 440 8 318 1.71e-60 201
Msa0615920 AT3G15720.4 37.356 348 186 11 116 454 2 326 1.82e-60 203
Msa0615920 AT1G43100.1 36.134 357 206 9 74 422 30 372 4.04e-60 203
Msa0615920 AT2G26620.1 36.056 355 207 9 74 422 30 370 1.94e-59 201
Msa0615920 AT3G07850.1 39.093 353 197 9 75 423 70 408 2.90e-59 201
Msa0615920 AT2G40310.1 35.955 356 206 9 75 422 31 372 3.56e-59 200
Msa0615920 AT3G07820.1 36.337 344 202 8 83 422 27 357 5.77e-59 199
Msa0615920 AT3G07830.1 35.503 338 202 7 88 422 34 358 6.79e-59 199
Msa0615920 AT3G07840.1 34.971 346 209 7 80 422 26 358 8.08e-59 199
Msa0615920 AT2G15460.1 35.475 358 207 9 74 422 30 372 1.28e-58 198
Msa0615920 AT4G32380.1 32.976 373 222 8 89 452 41 394 1.64e-58 199
Msa0615920 AT5G48140.1 34.911 338 203 7 88 422 34 357 1.66e-58 198
Msa0615920 AT2G15450.1 35.196 358 208 9 74 422 30 372 3.76e-58 197
Msa0615920 AT2G15470.1 34.916 358 209 9 74 422 30 372 1.30e-56 193
Msa0615920 AT4G35670.2 35.185 270 161 3 158 427 2 257 4.13e-53 180
Msa0615920 AT4G32370.1 36.860 293 163 6 89 373 49 327 1.14e-52 181
Msa0615920 AT4G32375.1 36.667 270 149 4 158 422 13 265 2.50e-50 177
Msa0615920 AT4G32380.2 29.459 370 198 8 89 452 41 353 6.28e-46 165
Msa0615920 AT4G33440.1 28.363 342 191 12 48 358 52 370 3.75e-23 102
Msa0615920 AT3G42950.1 26.842 380 233 12 73 422 64 428 7.50e-21 95.5
Msa0615920 AT3G62110.1 26.437 348 210 11 77 393 45 377 5.34e-20 92.8
Msa0615920 AT3G62110.2 26.437 348 210 11 77 393 45 377 5.34e-20 92.8
Msa0615920 AT1G19170.1 24.627 402 235 14 63 422 75 450 7.18e-20 92.8
Msa0615920 AT5G41870.1 26.000 350 206 12 47 364 21 349 4.20e-19 90.1
Msa0615920 AT5G49215.1 25.600 375 235 11 77 422 45 404 1.18e-17 85.5
Msa0615920 AT5G49215.2 25.600 375 235 11 77 422 43 402 1.44e-17 85.5
Msa0615920 AT4G23820.1 25.868 317 191 8 77 364 43 344 2.00e-17 84.7
Msa0615920 AT3G48950.1 23.962 313 192 8 77 358 47 344 5.20e-17 83.6
Msa0615920 AT2G23900.1 25.641 312 188 10 77 358 58 355 5.64e-16 80.5
Msa0615920 AT3G61490.5 24.839 310 192 10 77 358 62 358 1.17e-14 76.6
Msa0615920 AT3G61490.2 24.839 310 192 10 77 358 47 343 1.31e-14 76.3
Msa0615920 AT3G61490.3 24.839 310 192 10 77 358 47 343 1.31e-14 76.3
Msa0615920 AT3G61490.1 24.839 310 192 10 77 358 47 343 1.31e-14 76.3
Msa0615920 AT3G61490.4 24.839 310 192 10 77 358 160 456 1.34e-14 76.6
Msa0615920 AT4G23500.1 26.537 309 176 11 80 355 73 363 3.47e-13 72.0

Find 129 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4_2:+65534278 Msa0615920:CDS
TCTTAGGATTTGTCCCTATT+TGG 0.124261 4_2:+65534478 Msa0615920:three_prime_UTR
TCTACCTAATCAACCAAATT+TGG 0.144380 4_2:-65534371 None:intergenic
CAGTTCCACATGATATTTAA+TGG 0.196238 4_2:+65533280 Msa0615920:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4_2:-65534283 None:intergenic
TTCATGGACTATTTATTCTT+TGG 0.220446 4_2:+65531709 Msa0615920:CDS
ATGGGAAAATACACCCAAAT+AGG 0.222592 4_2:-65534492 None:intergenic
TTAATTACCATTGCTTATTA+AGG 0.227433 4_2:-65533410 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4_2:-65534235 None:intergenic
ATATTTGTGTTAAAGATAAT+TGG 0.246059 4_2:-65531577 None:intergenic
CAGTCCATTCACATACAAAT+TGG 0.246918 4_2:-65533239 None:intergenic
ATGATGTATTAGTCCAAATT+TGG 0.262749 4_2:+65534358 Msa0615920:three_prime_UTR
CTTAGGATTTGTCCCTATTT+GGG 0.283443 4_2:+65534479 Msa0615920:three_prime_UTR
TCTCAAATTAAATATTATAA+AGG 0.297000 4_2:-65531736 None:intergenic
CAGCAACTCATCGCTCACTA+TGG 0.305602 4_2:-65534511 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4_2:-65532459 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4_2:-65531885 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4_2:+65531927 Msa0615920:CDS
AACAATAGTATTGGTCTATA+AGG 0.326820 4_2:-65534024 None:intergenic
AACACAAATATTTCTGAAAC+AGG 0.328264 4_2:+65531589 Msa0615920:five_prime_UTR
TAGTCCAAATTTGGTTGATT+AGG 0.339571 4_2:+65534367 Msa0615920:three_prime_UTR
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4_2:+65532007 Msa0615920:CDS
TATATATTATATATTACATT+AGG 0.360280 4_2:+65534397 Msa0615920:three_prime_UTR
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4_2:-65532423 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4_2:-65534310 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4_2:-65531816 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4_2:+65533548 Msa0615920:intron
ACATGGATTCCATCAGTGTT+GGG 0.385395 4_2:-65533355 None:intergenic
CTGTACCATTAAAACTCATT+TGG 0.389055 4_2:-65532397 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4_2:+65534279 Msa0615920:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4_2:-65532338 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4_2:+65531890 Msa0615920:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4_2:-65533343 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4_2:-65532329 None:intergenic
TCCCATATGGATCTCATTCA+TGG 0.407523 4_2:+65531693 Msa0615920:exon
CATTTATACTTATTATTATG+TGG 0.415534 4_2:+65534579 Msa0615920:three_prime_UTR
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4_2:+65533609 Msa0615920:CDS
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4_2:-65531972 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4_2:-65534139 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4_2:-65532350 None:intergenic
TGGGAAAATACACCCAAATA+GGG 0.426962 4_2:-65534491 None:intergenic
CTTACTTGAAATACTAATCC+TGG 0.430828 4_2:-65532086 None:intergenic
AATATTTAATTTGAGAAATG+TGG 0.432106 4_2:+65531743 Msa0615920:CDS
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4_2:+65532416 Msa0615920:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4_2:-65534033 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4_2:+65532327 Msa0615920:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4_2:-65531850 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4_2:-65533356 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4_2:-65531879 None:intergenic
AATGGTACAGGAACCATTGA+AGG 0.452512 4_2:+65532410 Msa0615920:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4_2:+65531878 Msa0615920:CDS
GTCCATGAATGAGATCCATA+TGG 0.453446 4_2:-65531695 None:intergenic
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4_2:+65531996 Msa0615920:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4_2:+65532453 Msa0615920:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4_2:+65533982 Msa0615920:CDS
TGAGCGATGAGTTGCTGAAT+TGG 0.456799 4_2:+65534517 Msa0615920:three_prime_UTR
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4_2:+65534207 Msa0615920:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4_2:-65534280 None:intergenic
TTTCATATTTGTAGATGATA+CGG 0.467936 4_2:+65533203 Msa0615920:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4_2:+65533944 Msa0615920:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4_2:-65533573 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4_2:-65531878 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4_2:+65533376 Msa0615920:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4_2:-65533873 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4_2:+65533377 Msa0615920:CDS
TGAAATTGAAGAAAGAAGAA+TGG 0.492537 4_2:-65531547 None:intergenic
TCAGGTGGATGGTATGCTAA+TGG 0.494463 4_2:+65532304 Msa0615920:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4_2:+65533235 Msa0615920:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4_2:+65534283 Msa0615920:CDS
AAATCATGCTTTCACAATGT+AGG 0.499150 4_2:-65534554 None:intergenic
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4_2:+65533839 Msa0615920:intron
ATTTGAGAAATGTGGATGGA+AGG 0.500205 4_2:+65531751 Msa0615920:CDS
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4_2:+65533943 Msa0615920:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4_2:+65531877 Msa0615920:CDS
TCCAACAATTCACCTTCAAA+TGG 0.505935 4_2:-65534208 None:intergenic
AATACTAATCCTGGTTTGCA+TGG 0.507411 4_2:-65532077 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4_2:-65533306 None:intergenic
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4_2:+65533603 Msa0615920:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4_2:+65533578 Msa0615920:CDS
AGCAACTCATCGCTCACTAT+GGG 0.537725 4_2:-65534510 None:intergenic
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4_2:+65531933 Msa0615920:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4_2:+65533602 Msa0615920:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4_2:+65533289 Msa0615920:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4_2:+65533992 Msa0615920:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4_2:-65533572 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4_2:+65532429 Msa0615920:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4_2:-65533874 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4_2:+65533346 Msa0615920:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4_2:+65533554 Msa0615920:CDS
TTTAATTTGAGAAATGTGGA+TGG 0.556957 4_2:+65531747 Msa0615920:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4_2:+65533403 Msa0615920:CDS
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4_2:-65531809 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4_2:+65531970 Msa0615920:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4_2:+65532293 Msa0615920:intron
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4_2:+65532426 Msa0615920:CDS
TGAAGAAAGAAGAATGGCTA+AGG 0.575307 4_2:-65531541 None:intergenic
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4_2:-65532458 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4_2:-65531870 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4_2:-65533614 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4_2:+65534033 Msa0615920:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4_2:+65534300 Msa0615920:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4_2:-65533277 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4_2:+65532454 Msa0615920:CDS
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4_2:+65532354 Msa0615920:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4_2:+65533568 Msa0615920:CDS
CTCACAGCTGGACTTCCACA+TGG 0.605942 4_2:-65532718 None:intergenic
TCCATGAATGAGATCCATAT+GGG 0.606027 4_2:-65531694 None:intergenic
TCTTATGACTGTGTCACGGA+CGG 0.610881 4_2:-65533897 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4_2:+65533935 Msa0615920:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4_2:+65532314 Msa0615920:CDS
GGCAGCCATTAAATATCATG+TGG 0.622918 4_2:-65533285 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4_2:-65533354 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4_2:-65531861 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4_2:+65533555 Msa0615920:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4_2:-65531808 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4_2:-65531846 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4_2:-65534309 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4_2:-65533619 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4_2:+65532334 Msa0615920:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4_2:+65533590 Msa0615920:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4_2:+65533911 Msa0615920:CDS
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4_2:-65533901 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4_2:+65532703 Msa0615920:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4_2:+65532430 Msa0615920:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4_2:+65534174 Msa0615920:CDS
AGAATAAAGACATGGCAAGG+TGG 0.690276 4_2:+65533938 Msa0615920:CDS
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4_2:-65531894 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4_2:+65533930 Msa0615920:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4_2:+65534301 Msa0615920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTCAAATTAAATATTATAA+AGG - chr4_2:65531739-65531758 None:intergenic 10.0%
!! TCTATAAATAAATATTTACA+TGG - chr4_2:65532514-65532533 None:intergenic 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG + chr4_2:65533086-65533105 Msa0615920:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG + chr4_2:65533116-65533135 Msa0615920:intron 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG + chr4_2:65533117-65533136 Msa0615920:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG - chr4_2:65533724-65533743 None:intergenic 10.0%
!! ATATTTGTGTTAAAGATAAT+TGG - chr4_2:65531580-65531599 None:intergenic 15.0%
!!! AATATTTAATTTGAGAAATG+TGG + chr4_2:65531743-65531762 Msa0615920:CDS 15.0%
!! TCAACTTAATAAAATATGAT+TGG + chr4_2:65532195-65532214 Msa0615920:intron 15.0%
!! ATTCTATATGTAATTAATAG+AGG + chr4_2:65532771-65532790 Msa0615920:intron 15.0%
!! TTCTATATGTAATTAATAGA+GGG + chr4_2:65532772-65532791 Msa0615920:intron 15.0%
!! ATGTCAAAATTATATCAATT+AGG + chr4_2:65533027-65533046 Msa0615920:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG + chr4_2:65533028-65533047 Msa0615920:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG - chr4_2:65533093-65533112 None:intergenic 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG + chr4_2:65533118-65533137 Msa0615920:intron 15.0%
!!! ATTATATGTTTTGATTGTTA+TGG + chr4_2:65534438-65534457 Msa0615920:three_prime_UTR 15.0%
!! CATTTATACTTATTATTATG+TGG + chr4_2:65534579-65534598 Msa0615920:three_prime_UTR 15.0%
!!! ATCATAAAAGTGTAATTTTC+TGG - chr4_2:65532035-65532054 None:intergenic 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG + chr4_2:65532053-65532072 Msa0615920:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG + chr4_2:65532286-65532305 Msa0615920:intron 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG + chr4_2:65532568-65532587 Msa0615920:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG - chr4_2:65532668-65532687 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG - chr4_2:65532849-65532868 None:intergenic 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG - chr4_2:65532878-65532897 None:intergenic 20.0%
!! TAAACATAAATCAGTCATTA+GGG - chr4_2:65532908-65532927 None:intergenic 20.0%
!! ATAAACATAAATCAGTCATT+AGG - chr4_2:65532909-65532928 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG - chr4_2:65533001-65533020 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG + chr4_2:65533056-65533075 Msa0615920:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG + chr4_2:65533057-65533076 Msa0615920:intron 20.0%
!! TTTCATATTTGTAGATGATA+CGG + chr4_2:65533203-65533222 Msa0615920:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG - chr4_2:65533413-65533432 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG + chr4_2:65533413-65533432 Msa0615920:intron 20.0%
!! TATAATAACATGATTTGAAC+AGG + chr4_2:65533527-65533546 Msa0615920:intron 20.0%
!! AAAATTTGAGAACATACAAA+TGG + chr4_2:65533982-65534001 Msa0615920:CDS 20.0%
! TGAAATTGAAGAAAGAAGAA+TGG - chr4_2:65531550-65531569 None:intergenic 25.0%
!!! AACACAAATATTTCTGAAAC+AGG + chr4_2:65531589-65531608 Msa0615920:five_prime_UTR 25.0%
!!! ATTTTGTTGTAGCACAATAT+TGG + chr4_2:65531651-65531670 Msa0615920:five_prime_UTR 25.0%
!! TTCATGGACTATTTATTCTT+TGG + chr4_2:65531709-65531728 Msa0615920:CDS 25.0%
!!! TTTAATTTGAGAAATGTGGA+TGG + chr4_2:65531747-65531766 Msa0615920:CDS 25.0%
!! ATTTTCGATGTTACATCATT+TGG + chr4_2:65531918-65531937 Msa0615920:CDS 25.0%
! AAAATTGAAATGTAATCAGG+TGG + chr4_2:65532289-65532308 Msa0615920:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG - chr4_2:65532661-65532680 None:intergenic 25.0%
! CACTAACCAAAAATAAAACT+TGG + chr4_2:65532659-65532678 Msa0615920:intron 25.0%
!! AAATAAAACTTGGCATTTTC+AGG + chr4_2:65532669-65532688 Msa0615920:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG + chr4_2:65532670-65532689 Msa0615920:intron 25.0%
! ATTAGGGACTAAAAACAAAA+TGG - chr4_2:65532892-65532911 None:intergenic 25.0%
! TTTAATATAACATGGACTCA+GGG - chr4_2:65532993-65533012 None:intergenic 25.0%
!! TAGAAACAAAGTATCAAACA+GGG - chr4_2:65533460-65533479 None:intergenic 25.0%
!! ATAGAAACAAAGTATCAAAC+AGG - chr4_2:65533461-65533480 None:intergenic 25.0%
! TGAATAGAAAAATTAGGATC+TGG - chr4_2:65533718-65533737 None:intergenic 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG + chr4_2:65533830-65533849 Msa0615920:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG - chr4_2:65534027-65534046 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG + chr4_2:65534278-65534297 Msa0615920:CDS 25.0%
! ACCTCCAATTAATTGTTTAA+GGG + chr4_2:65534279-65534298 Msa0615920:CDS 25.0%
! ATGATGTATTAGTCCAAATT+TGG + chr4_2:65534358-65534377 Msa0615920:three_prime_UTR 25.0%
!!! TTTTTTTATGTGTGCATCTT+AGG + chr4_2:65534462-65534481 Msa0615920:three_prime_UTR 25.0%
! CAAATTTTGAACTTCCCATA+TGG + chr4_2:65531680-65531699 Msa0615920:exon 30.0%
CTTACTTGAAATACTAATCC+TGG - chr4_2:65532089-65532108 None:intergenic 30.0%
!! CTTGACCAAATGAGTTTTAA+TGG + chr4_2:65532392-65532411 Msa0615920:CDS 30.0%
CTGTACCATTAAAACTCATT+TGG - chr4_2:65532400-65532419 None:intergenic 30.0%
!! CAAATGAGTTTTAATGGTAC+AGG + chr4_2:65532398-65532417 Msa0615920:CDS 30.0%
TATTTAATGACTGAGCTGAA+TGG - chr4_2:65532749-65532768 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG - chr4_2:65532992-65533011 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG - chr4_2:65532994-65533013 None:intergenic 30.0%
TTAAATATCATGTGGAACTG+AGG - chr4_2:65533280-65533299 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG + chr4_2:65533280-65533299 Msa0615920:CDS 30.0%
ACATATTGATCATTACTCCT+TGG - chr4_2:65533309-65533328 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG + chr4_2:65533377-65533396 Msa0615920:CDS 30.0%
TACAACTCCTTAATAAGCAA+TGG + chr4_2:65533403-65533422 Msa0615920:CDS 30.0%
CAAACAGGGTAAGTAAATAT+TGG - chr4_2:65533446-65533465 None:intergenic 30.0%
! ATGGACTAAGAATAAAGACA+TGG + chr4_2:65533930-65533949 Msa0615920:CDS 30.0%
AACATACAAATGGAGAATGT+AGG + chr4_2:65533992-65534011 Msa0615920:CDS 30.0%
CCTTTGTCAAACAATAGTAT+TGG - chr4_2:65534036-65534055 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG + chr4_2:65534033-65534052 Msa0615920:CDS 30.0%
TGTGTCCTACAAAAACATTA+AGG + chr4_2:65534093-65534112 Msa0615920:CDS 30.0%
GTGTCCTACAAAAACATTAA+GGG + chr4_2:65534094-65534113 Msa0615920:CDS 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG - chr4_2:65534101-65534120 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG - chr4_2:65534142-65534161 None:intergenic 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr4_2:65534237-65534256 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG - chr4_2:65534283-65534302 None:intergenic 30.0%
CCTTCCCTTAAACAATTAAT+TGG - chr4_2:65534286-65534305 None:intergenic 30.0%
CCAATTAATTGTTTAAGGGA+AGG + chr4_2:65534283-65534302 Msa0615920:CDS 30.0%
TAGTCCAAATTTGGTTGATT+AGG + chr4_2:65534367-65534386 Msa0615920:three_prime_UTR 30.0%
TCTACCTAATCAACCAAATT+TGG - chr4_2:65534374-65534393 None:intergenic 30.0%
!!! GCTGAATTGGTTCATTTTTT+TGG + chr4_2:65534530-65534549 Msa0615920:three_prime_UTR 30.0%
AAATCATGCTTTCACAATGT+AGG - chr4_2:65534557-65534576 None:intergenic 30.0%
TGAAGAAAGAAGAATGGCTA+AGG - chr4_2:65531544-65531563 None:intergenic 35.0%
TCCATGAATGAGATCCATAT+GGG - chr4_2:65531697-65531716 None:intergenic 35.0%
ATTTGAGAAATGTGGATGGA+AGG + chr4_2:65531751-65531770 Msa0615920:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG - chr4_2:65531790-65531809 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG - chr4_2:65531811-65531830 None:intergenic 35.0%
TTGAAGACTTAGAAGGAACA+AGG - chr4_2:65531812-65531831 None:intergenic 35.0%
GGATCATTTGAAGACTTAGA+AGG - chr4_2:65531819-65531838 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG + chr4_2:65531927-65531946 Msa0615920:CDS 35.0%
! AATACTAATCCTGGTTTGCA+TGG - chr4_2:65532080-65532099 None:intergenic 35.0%
ATTGGACGTATGTGTAAAAC+TGG + chr4_2:65532213-65532232 Msa0615920:intron 35.0%
TTGAAATGTAATCAGGTGGA+TGG + chr4_2:65532293-65532312 Msa0615920:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG - chr4_2:65532426-65532445 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG + chr4_2:65532426-65532445 Msa0615920:CDS 35.0%
TCAAAAAAGTTTACCCTGTG+AGG - chr4_2:65532470-65532489 None:intergenic 35.0%
TAATATAACATGGACTCAGG+GGG - chr4_2:65532991-65533010 None:intergenic 35.0%
AGCTCCAATTTGTATGTGAA+TGG + chr4_2:65533235-65533254 Msa0615920:CDS 35.0%
CAGTCCATTCACATACAAAT+TGG - chr4_2:65533242-65533261 None:intergenic 35.0%
ATGATATTTAATGGCTGCCA+AGG + chr4_2:65533289-65533308 Msa0615920:CDS 35.0%
! CATCTTGTGAGTTTTCAACA+TGG - chr4_2:65533375-65533394 None:intergenic 35.0%
GTTGAAAACTCACAAGATGT+TGG + chr4_2:65533376-65533395 Msa0615920:CDS 35.0%
GCTGCTTGCTTACATAAAAA+TGG - chr4_2:65533503-65533522 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr4_2:65533548-65533567 Msa0615920:intron 35.0%
! TATTTCCACATTTGAAGTCC+CGG - chr4_2:65533576-65533595 None:intergenic 35.0%
ACTTCAAATGTGGAAATAGC+TGG + chr4_2:65533578-65533597 Msa0615920:CDS 35.0%
! TAAGTTTGAGACACATGCTT+GGG - chr4_2:65533876-65533895 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG - chr4_2:65533877-65533896 None:intergenic 35.0%
ATTCTCTTATGACTGTGTCA+CGG - chr4_2:65533904-65533923 None:intergenic 35.0%
GTCATAAGAGAATCAGACAA+TGG + chr4_2:65533911-65533930 Msa0615920:CDS 35.0%
CTAAGAATAAAGACATGGCA+AGG + chr4_2:65533935-65533954 Msa0615920:CDS 35.0%
TACAAACATAACACTCTCTG+AGG + chr4_2:65534174-65534193 Msa0615920:CDS 35.0%
TCCAACAATTCACCTTCAAA+TGG - chr4_2:65534211-65534230 None:intergenic 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr4_2:65534238-65534257 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr4_2:65534235-65534254 Msa0615920:CDS 35.0%
TCTTAGGATTTGTCCCTATT+TGG + chr4_2:65534478-65534497 Msa0615920:three_prime_UTR 35.0%
CTTAGGATTTGTCCCTATTT+GGG + chr4_2:65534479-65534498 Msa0615920:three_prime_UTR 35.0%
TGGGAAAATACACCCAAATA+GGG - chr4_2:65534494-65534513 None:intergenic 35.0%
ATGGGAAAATACACCCAAAT+AGG - chr4_2:65534495-65534514 None:intergenic 35.0%
TCCCATATGGATCTCATTCA+TGG + chr4_2:65531693-65531712 Msa0615920:exon 40.0%
GTCCATGAATGAGATCCATA+TGG - chr4_2:65531698-65531717 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG - chr4_2:65531779-65531798 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG - chr4_2:65531840-65531859 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG - chr4_2:65531873-65531892 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG + chr4_2:65531877-65531896 Msa0615920:CDS 40.0%
! TCATTTGGAGCAATTGGAGA+TGG + chr4_2:65531933-65531952 Msa0615920:CDS 40.0%
TTCCATGCTTTCTTGAATGC+AGG - chr4_2:65531975-65531994 None:intergenic 40.0%
AGGCAGATAGCAGAAATCAA+TGG + chr4_2:65532354-65532373 Msa0615920:CDS 40.0%
AATGGTACAGGAACCATTGA+AGG + chr4_2:65532410-65532429 Msa0615920:CDS 40.0%
ACAGGAACCATTGAAGGAAA+TGG + chr4_2:65532416-65532435 Msa0615920:CDS 40.0%
AAGGAAATGGAGATCAATGG+TGG + chr4_2:65532429-65532448 Msa0615920:CDS 40.0%
AGGAAATGGAGATCAATGGT+GGG + chr4_2:65532430-65532449 Msa0615920:CDS 40.0%
! CCTGACAAAGTTTTTCCATC+TGG - chr4_2:65532697-65532716 None:intergenic 40.0%
CCAGATGGAAAAACTTTGTC+AGG + chr4_2:65532694-65532713 Msa0615920:CDS 40.0%
AAAACTTTGTCAGGACCATG+TGG + chr4_2:65532703-65532722 Msa0615920:CDS 40.0%
!! TGAATGGTTTTACTCACAGC+TGG - chr4_2:65532733-65532752 None:intergenic 40.0%
GGCAGCCATTAAATATCATG+TGG - chr4_2:65533288-65533307 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG - chr4_2:65533335-65533354 None:intergenic 40.0%
ACATGGATTCCATCAGTGTT+GGG - chr4_2:65533358-65533377 None:intergenic 40.0%
AACATGGATTCCATCAGTGT+TGG - chr4_2:65533359-65533378 None:intergenic 40.0%
GATTGCATTTCAATTGGACC+CGG + chr4_2:65533554-65533573 Msa0615920:CDS 40.0%
ATTGCATTTCAATTGGACCC+GGG + chr4_2:65533555-65533574 Msa0615920:CDS 40.0%
! ATTTCCACATTTGAAGTCCC+GGG - chr4_2:65533575-65533594 None:intergenic 40.0%
GAAATAGCTGGTGTAACTTG+TGG + chr4_2:65533590-65533609 Msa0615920:CDS 40.0%
ATGTAATGTACCTAATCCCG+TGG - chr4_2:65533622-65533641 None:intergenic 40.0%
CACTACCAAGTGAAACAACA+TGG + chr4_2:65533680-65533699 Msa0615920:intron 40.0%
!! GCAAACCATGTTGTTTCACT+TGG - chr4_2:65533688-65533707 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG + chr4_2:65533839-65533858 Msa0615920:intron 40.0%
AGAATAAAGACATGGCAAGG+TGG + chr4_2:65533938-65533957 Msa0615920:CDS 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG + chr4_2:65534196-65534215 Msa0615920:CDS 40.0%
GCCATTTGAAGGTGAATTGT+TGG + chr4_2:65534207-65534226 Msa0615920:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG + chr4_2:65534224-65534243 Msa0615920:CDS 40.0%
ACACTAGGAGAAGAGTTGGA+AGG - chr4_2:65531849-65531868 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG - chr4_2:65531853-65531872 None:intergenic 45.0%
TAAGGGTCAGAAGGAACACT+AGG - chr4_2:65531864-65531883 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG - chr4_2:65531881-65531900 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG + chr4_2:65531878-65531897 Msa0615920:CDS 45.0%
TCCCGAGGATCATTTGGATA+AGG - chr4_2:65531882-65531901 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG + chr4_2:65531970-65531989 Msa0615920:CDS 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG + chr4_2:65532007-65532026 Msa0615920:CDS 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG + chr4_2:65532068-65532087 Msa0615920:CDS 45.0%
TCAGGTGGATGGTATGCTAA+TGG + chr4_2:65532304-65532323 Msa0615920:intron 45.0%
TGATTTCTGCTATCTGCCTC+TGG - chr4_2:65532353-65532372 None:intergenic 45.0%
TTTACCCTGTGAGGTTTGCA+GGG - chr4_2:65532461-65532480 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG - chr4_2:65533346-65533365 None:intergenic 45.0%
AAACTTAGCCCCAACACTGA+TGG + chr4_2:65533346-65533365 Msa0615920:CDS 45.0%
CATGGATTCCATCAGTGTTG+GGG - chr4_2:65533357-65533376 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG - chr4_2:65533900-65533919 None:intergenic 45.0%
AAAGACATGGCAAGGTGGAA+TGG + chr4_2:65533943-65533962 Msa0615920:CDS 45.0%
AAGACATGGCAAGGTGGAAT+GGG + chr4_2:65533944-65533963 Msa0615920:CDS 45.0%
AAAGATCACCCACAGTCTCA+GGG - chr4_2:65534312-65534331 None:intergenic 45.0%
AGCAACTCATCGCTCACTAT+GGG - chr4_2:65534513-65534532 None:intergenic 45.0%
TGAGCGATGAGTTGCTGAAT+TGG + chr4_2:65534517-65534536 Msa0615920:three_prime_UTR 45.0%
!! TATATATTATATATTACATT+AGG + chr4_2:65534397-65534416 Msa0615920:three_prime_UTR 5.0%
GACGAATCCCGAGGATCATT+TGG - chr4_2:65531888-65531907 None:intergenic 50.0%
TGATCCTCGGGATTCGTCTT+CGG + chr4_2:65531890-65531909 Msa0615920:CDS 50.0%
GGTATGCTAATGGCACCAGA+TGG + chr4_2:65532314-65532333 Msa0615920:CDS 50.0%
CACCAGATGGACCTGATTCA+TGG + chr4_2:65532327-65532346 Msa0615920:CDS 50.0%
ATCTTCCCTGCAAACCTCAC+AGG + chr4_2:65532453-65532472 Msa0615920:CDS 50.0%
TCTTCCCTGCAAACCTCACA+GGG + chr4_2:65532454-65532473 Msa0615920:CDS 50.0%
GTTTACCCTGTGAGGTTTGC+AGG - chr4_2:65532462-65532481 None:intergenic 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG + chr4_2:65532679-65532698 Msa0615920:intron 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG + chr4_2:65533602-65533621 Msa0615920:CDS 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG + chr4_2:65533603-65533622 Msa0615920:CDS 50.0%
ATGTACCTAATCCCGTGGCT+AGG - chr4_2:65533617-65533636 None:intergenic 50.0%
GAAAGATCACCCACAGTCTC+AGG - chr4_2:65534313-65534332 None:intergenic 50.0%
CAGCAACTCATCGCTCACTA+TGG - chr4_2:65534514-65534533 None:intergenic 50.0%
GCAGCTTGTGCTGTAGAGTC+AGG + chr4_2:65531996-65532015 Msa0615920:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG - chr4_2:65532332-65532351 None:intergenic 55.0%
TGGACCTGATTCATGGCCAG+AGG + chr4_2:65532334-65532353 Msa0615920:CDS 55.0%
TCTGCCTCTGGCCATGAATC+AGG - chr4_2:65532341-65532360 None:intergenic 55.0%
CTCACAGCTGGACTTCCACA+TGG - chr4_2:65532721-65532740 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG + chr4_2:65533568-65533587 Msa0615920:CDS 55.0%
GAAGGTGACCCTGAGACTGT+GGG + chr4_2:65534301-65534320 Msa0615920:CDS 55.0%
CAGTCCGAAGACGAATCCCG+AGG - chr4_2:65531897-65531916 None:intergenic 60.0%
GTGGTCCTAGCCACGGGATT+AGG + chr4_2:65533609-65533628 Msa0615920:CDS 60.0%
GGAAGGTGACCCTGAGACTG+TGG + chr4_2:65534300-65534319 Msa0615920:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 65531526 65534617 65531526 ID=Msa0615920;Name=Msa0615920
chr4_2 mRNA 65531526 65534617 65531526 ID=Msa0615920-mRNA-1;Parent=Msa0615920;Name=Msa0615920-mRNA-1;_AED=0.12;_eAED=0.12;_QI=173|1|1|1|1|1|6|263|471
chr4_2 exon 65531526 65532103 65531526 ID=Msa0615920-mRNA-1:exon:18573;Parent=Msa0615920-mRNA-1
chr4_2 exon 65532308 65532475 65532308 ID=Msa0615920-mRNA-1:exon:18574;Parent=Msa0615920-mRNA-1
chr4_2 exon 65532691 65532735 65532691 ID=Msa0615920-mRNA-1:exon:18575;Parent=Msa0615920-mRNA-1
chr4_2 exon 65533217 65533424 65533217 ID=Msa0615920-mRNA-1:exon:18576;Parent=Msa0615920-mRNA-1
chr4_2 exon 65533549 65533630 65533549 ID=Msa0615920-mRNA-1:exon:18577;Parent=Msa0615920-mRNA-1
chr4_2 exon 65533847 65534617 65533847 ID=Msa0615920-mRNA-1:exon:18578;Parent=Msa0615920-mRNA-1
chr4_2 five_prime_UTR 65531526 65531698 65531526 ID=Msa0615920-mRNA-1:five_prime_utr;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65531699 65532103 65531699 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65532308 65532475 65532308 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65532691 65532735 65532691 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65533217 65533424 65533217 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65533549 65533630 65533549 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 CDS 65533847 65534354 65533847 ID=Msa0615920-mRNA-1:cds;Parent=Msa0615920-mRNA-1
chr4_2 three_prime_UTR 65534355 65534617 65534355 ID=Msa0615920-mRNA-1:three_prime_utr;Parent=Msa0615920-mRNA-1
Gene Sequence

>Msa0615920

ATGGATCTCATTCATGGACTATTTATTCTTTGGATTACCTTTATAATATTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGATTAGTATTTCAAGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTTTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCTATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTGAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTAAATCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>Msa0615920

MDLIHGLFILWITFIIFNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGLVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSFNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN*