AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0699010

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699010 MtrunA17_Chr4g0052621 95.966 471 9 1 1 471 1 461 0.0 931
Msa0699010 MtrunA17_Chr2g0293681 68.153 471 108 4 1 471 1 429 0.0 627
Msa0699010 MtrunA17_Chr1g0194231 63.077 390 142 1 74 461 80 469 0.0 541
Msa0699010 MtrunA17_Chr7g0270361 60.382 419 162 3 53 469 46 462 0.0 537
Msa0699010 MtrunA17_Chr5g0413411 43.763 473 225 7 21 458 56 522 2.37e-126 377
Msa0699010 MtrunA17_Chr8g0365891 46.838 427 193 7 58 458 91 509 9.60e-126 375
Msa0699010 MtrunA17_Chr6g0449961 46.599 397 194 6 75 470 45 424 2.58e-122 363
Msa0699010 MtrunA17_Chr5g0413141 40.000 400 227 6 60 456 50 439 5.41e-104 317
Msa0699010 MtrunA17_Chr8g0365901 40.863 394 220 6 74 465 64 446 1.14e-101 311
Msa0699010 MtrunA17_Chr2g0332801 40.263 380 214 7 74 450 58 427 9.27e-93 288
Msa0699010 MtrunA17_Chr1g0173451 43.380 355 182 6 70 422 71 408 2.52e-92 287
Msa0699010 MtrunA17_Chr4g0020771 43.251 363 191 7 69 427 68 419 4.37e-90 281
Msa0699010 MtrunA17_Chr2g0332971 36.031 383 233 7 75 453 28 402 1.37e-79 253
Msa0699010 MtrunA17_Chr3g0089021 41.873 363 187 8 76 432 72 416 2.99e-77 248
Msa0699010 MtrunA17_Chr7g0257701 39.589 341 155 4 69 373 59 384 2.87e-73 236
Msa0699010 MtrunA17_Chr3g0121891 35.961 406 232 10 53 453 13 395 4.56e-73 236
Msa0699010 MtrunA17_Chr2g0303941 37.629 388 223 9 68 453 21 391 5.82e-73 235
Msa0699010 MtrunA17_Chr7g0257721 45.387 271 138 4 69 338 70 331 2.05e-71 229
Msa0699010 MtrunA17_Chr4g0043661 35.616 365 208 8 75 428 23 371 5.46e-69 225
Msa0699010 MtrunA17_Chr5g0418411 36.272 397 225 12 66 455 23 398 1.49e-68 224
Msa0699010 MtrunA17_Chr4g0043431 37.173 382 221 11 75 453 35 400 3.68e-67 220
Msa0699010 MtrunA17_Chr6g0461181 33.729 421 221 10 75 453 30 434 4.28e-67 221
Msa0699010 MtrunA17_Chr4g0018151 35.606 396 227 8 66 455 69 442 4.38e-67 221
Msa0699010 MtrunA17_Chr1g0195221 36.387 382 215 13 68 438 28 392 7.22e-67 220
Msa0699010 MtrunA17_Chr7g0230891 38.177 351 199 11 75 422 37 372 2.07e-66 218
Msa0699010 MtrunA17_Chr8g0348981 35.754 358 210 7 74 424 26 370 2.34e-66 218
Msa0699010 MtrunA17_Chr7g0228411 34.328 402 230 12 74 452 39 429 3.04e-66 219
Msa0699010 MtrunA17_Chr8g0337571 35.733 389 219 11 70 453 26 388 2.04e-65 216
Msa0699010 MtrunA17_Chr2g0307811 35.754 358 217 8 74 428 24 371 5.06e-65 215
Msa0699010 MtrunA17_Chr3g0121881 35.278 360 211 9 75 428 33 376 1.09e-64 214
Msa0699010 MtrunA17_Chr2g0307831 35.227 352 216 7 74 422 24 366 1.16e-64 214
Msa0699010 MtrunA17_Chr2g0307821 35.227 352 216 7 74 422 24 366 1.16e-64 214
Msa0699010 MtrunA17_Chr8g0337581 32.648 389 232 10 70 453 26 389 7.68e-61 204
Msa0699010 MtrunA17_Chr1g0150301 32.984 382 237 9 75 453 27 392 7.06e-60 201
Msa0699010 MtrunA17_Chr2g0291841 38.580 324 179 8 107 422 23 334 1.30e-59 199
Msa0699010 MtrunA17_Chr1g0150291 32.552 384 240 9 75 455 45 412 1.96e-58 198
Msa0699010 MtrunA17_Chr3g0100091 33.419 389 193 9 75 461 30 354 4.21e-58 195
Msa0699010 MtrunA17_Chr4g0067631 33.249 394 242 9 67 450 38 420 1.76e-57 196
Msa0699010 MtrunA17_Chr5g0447901 35.276 326 181 7 74 388 24 330 2.03e-56 190
Msa0699010 MtrunA17_Chr2g0295451 34.839 310 186 6 74 375 52 353 4.63e-53 183
Msa0699010 MtrunA17_Chr1g0149241 31.510 384 213 10 75 455 28 364 3.13e-51 177
Msa0699010 MtrunA17_Chr3g0093011 38.800 250 147 4 208 453 14 261 2.23e-47 164
Msa0699010 MtrunA17_Chr8g0337511 38.618 246 144 3 207 452 4 242 2.71e-47 163
Msa0699010 MtrunA17_Chr8g0337521 35.294 272 148 8 70 337 26 273 2.07e-46 163
Msa0699010 MtrunA17_Chr8g0336481 35.472 265 147 6 133 394 13 256 4.11e-46 161
Msa0699010 MtrunA17_Chr5g0425811 33.096 281 167 6 93 369 3 266 1.11e-43 155
Msa0699010 MtrunA17_Chr8g0337601 37.959 245 144 5 210 453 3 240 1.84e-40 145
Msa0699010 MtrunA17_Chr8g0337351 43.275 171 95 2 218 386 1 171 1.21e-35 130
Msa0699010 MtrunA17_Chr8g0337551 46.094 128 69 0 210 337 7 134 6.82e-28 108
Msa0699010 MtrunA17_Chr7g0273191 37.179 156 89 3 179 332 2 150 3.08e-27 106
Msa0699010 MtrunA17_Chr4g0070281 26.961 408 226 15 56 422 61 437 2.56e-24 105
Msa0699010 MtrunA17_Chr2g0301091 27.154 383 228 14 73 422 54 418 2.80e-23 102
Msa0699010 MtrunA17_Chr5g0442221 27.532 316 188 9 77 364 46 348 1.82e-18 87.8
Msa0699010 MtrunA17_Chr1g0202641 25.788 349 212 10 77 391 557 892 3.14e-18 88.2
Msa0699010 MtrunA17_Chr1g0202641 25.641 351 210 14 77 391 57 392 1.74e-16 82.4
Msa0699010 MtrunA17_Chr3g0093021 35.714 112 67 1 74 185 26 132 9.60e-18 80.9
Msa0699010 MtrunA17_Chr8g0337541 34.146 123 71 5 70 188 26 142 1.47e-17 79.7
Msa0699010 MtrunA17_Chr3g0095221 26.074 326 200 10 66 364 39 350 2.24e-17 84.3
Msa0699010 MtrunA17_Chr7g0270821 26.935 323 189 10 77 368 45 351 4.11e-17 83.6
Msa0699010 MtrunA17_Chr1g0193961 27.414 321 189 9 77 368 45 350 4.39e-17 83.6
Msa0699010 MtrunA17_Chr3g0130251 25.393 382 226 13 7 358 22 374 6.10e-17 83.2
Msa0699010 MtrunA17_Chr7g0255311 25.962 312 186 10 77 358 65 361 1.09e-16 82.4
Msa0699010 MtrunA17_Chr2g0330221 28.723 188 118 6 70 255 20 193 1.11e-16 79.0
Msa0699010 MtrunA17_Chr8g0387391 26.183 317 179 12 77 358 46 342 1.29e-16 82.0
Msa0699010 MtrunA17_Chr1g0202651 24.069 349 218 9 77 391 57 392 1.37e-16 82.0
Msa0699010 MtrunA17_Chr8g0348491 24.340 341 216 10 80 391 60 387 5.12e-15 77.0
Msa0699010 MtrunA17_Chr2g0295241 27.190 331 186 13 65 362 31 339 1.15e-14 75.9
Msa0699010 MtrunA17_Chr7g0273181 47.126 87 43 3 355 440 1 85 2.17e-14 69.7
Msa0699010 MtrunA17_Chr4g0067811 24.922 321 196 9 77 367 42 347 3.10e-14 74.7
Msa0699010 MtrunA17_Chr1g0202621 24.148 352 208 12 77 391 50 379 4.72e-14 73.9
Msa0699010 MtrunA17_Chr8g0373171 37.209 86 54 0 207 292 6 91 5.32e-14 68.6
Msa0699010 MtrunA17_Chr7g0248451 25.723 311 188 10 77 358 57 353 1.22e-13 72.8
Msa0699010 MtrunA17_Chr7g0257711 41.935 93 51 2 338 428 1 92 2.57e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0699010 AT1G48100.1 68.898 463 139 3 1 458 1 463 0.0 659
Msa0699010 AT1G02460.1 61.381 391 146 3 74 459 88 478 5.96e-180 513
Msa0699010 AT1G56710.1 56.968 409 170 4 54 459 28 433 2.42e-169 484
Msa0699010 AT4G01890.1 55.583 403 173 3 63 460 53 454 1.21e-166 478
Msa0699010 AT5G14650.1 47.436 390 189 6 75 461 51 427 4.40e-121 361
Msa0699010 AT1G60590.1 43.855 415 200 6 75 462 123 531 3.62e-118 357
Msa0699010 AT1G10640.1 44.988 409 195 6 74 458 118 520 1.73e-116 352
Msa0699010 AT3G26610.1 38.925 465 256 10 1 457 7 451 6.28e-107 326
Msa0699010 AT1G80170.1 42.670 382 207 6 71 449 49 421 1.55e-106 324
Msa0699010 AT1G70500.1 41.414 396 219 6 74 467 66 450 6.84e-106 323
Msa0699010 AT1G23460.2 41.045 402 220 7 62 459 30 418 3.23e-100 307
Msa0699010 AT1G23460.1 41.045 402 220 7 62 459 54 442 5.52e-100 308
Msa0699010 AT3G57510.1 45.299 351 175 7 77 422 70 408 1.04e-93 290
Msa0699010 AT3G07970.1 39.173 411 232 9 35 441 33 429 9.87e-91 283
Msa0699010 AT2G41850.1 42.571 350 186 6 77 422 70 408 7.34e-88 275
Msa0699010 AT1G05660.1 38.144 388 222 8 70 455 23 394 3.06e-78 249
Msa0699010 AT1G05650.1 37.436 390 226 8 68 455 21 394 3.45e-76 244
Msa0699010 AT3G15720.3 38.718 390 206 12 75 454 23 389 2.71e-75 243
Msa0699010 AT3G15720.1 38.718 390 206 12 75 454 24 390 2.76e-75 244
Msa0699010 AT2G43890.1 37.240 384 223 11 67 448 21 388 3.01e-75 241
Msa0699010 AT4G35670.1 36.236 356 209 6 75 427 24 364 6.88e-75 241
Msa0699010 AT5G14650.2 45.113 266 134 3 75 339 51 305 4.07e-74 236
Msa0699010 AT2G43870.1 39.316 351 196 7 75 422 21 357 1.13e-73 237
Msa0699010 AT3G15720.2 38.974 390 202 13 75 454 23 386 8.07e-73 237
Msa0699010 AT2G43880.1 37.337 383 217 10 75 453 31 394 1.82e-72 234
Msa0699010 AT5G44840.1 34.492 374 219 8 74 440 23 377 1.36e-71 232
Msa0699010 AT1G80170.2 42.435 271 146 4 71 339 49 311 1.85e-71 229
Msa0699010 AT2G33160.1 37.871 404 224 11 56 452 19 402 3.86e-70 235
Msa0699010 AT5G27530.1 37.500 360 195 7 74 428 52 386 4.93e-70 230
Msa0699010 AT1G80140.1 34.857 350 210 6 107 453 2 336 4.97e-70 226
Msa0699010 AT3G59850.1 37.995 379 213 11 75 448 23 384 2.01e-69 226
Msa0699010 AT1G02790.1 36.872 358 203 8 68 422 45 382 4.87e-69 226
Msa0699010 AT3G59850.2 39.377 353 194 10 75 422 67 404 4.91e-69 226
Msa0699010 AT1G65570.1 37.760 384 217 12 75 453 31 397 1.93e-68 224
Msa0699010 AT2G43860.1 37.240 384 222 11 75 455 33 400 1.21e-67 222
Msa0699010 AT1G78400.1 37.151 358 200 9 74 423 34 374 3.99e-64 213
Msa0699010 AT1G17150.1 36.364 385 225 8 74 453 32 401 4.58e-64 213
Msa0699010 AT1G43080.1 36.975 357 203 9 74 422 30 372 2.65e-63 211
Msa0699010 AT4G13760.1 36.415 357 205 9 74 422 30 372 2.70e-63 211
Msa0699010 AT5G17200.1 34.307 411 220 12 71 455 35 421 1.32e-62 209
Msa0699010 AT1G43090.1 36.975 357 203 9 74 422 30 372 1.34e-62 210
Msa0699010 AT5G39910.1 31.579 399 191 10 74 454 35 369 1.96e-61 205
Msa0699010 AT3G14040.1 39.660 353 195 9 75 423 71 409 3.32e-61 206
Msa0699010 AT5G44830.1 32.840 338 197 6 106 440 8 318 9.52e-61 202
Msa0699010 AT2G43880.2 39.189 296 166 5 75 369 31 313 9.70e-61 202
Msa0699010 AT1G43100.1 36.415 357 205 9 74 422 30 372 1.14e-60 205
Msa0699010 AT4G18180.1 36.464 362 211 8 68 422 32 381 1.47e-60 204
Msa0699010 AT3G15720.4 37.356 348 186 11 116 454 2 326 1.90e-60 203
Msa0699010 AT2G26620.1 36.338 355 206 9 74 422 30 370 1.12e-59 201
Msa0699010 AT2G40310.1 36.236 356 205 9 75 422 31 372 1.55e-59 201
Msa0699010 AT3G07850.1 39.093 353 197 9 75 423 70 408 1.83e-59 202
Msa0699010 AT3G07820.1 36.628 344 201 8 83 422 27 357 3.74e-59 199
Msa0699010 AT3G07830.1 35.503 338 202 7 88 422 34 358 4.36e-59 199
Msa0699010 AT3G07840.1 34.971 346 209 7 80 422 26 358 5.09e-59 199
Msa0699010 AT2G15460.1 35.754 358 206 9 74 422 30 372 5.50e-59 199
Msa0699010 AT4G32380.1 33.244 373 221 8 89 452 41 394 7.01e-59 201
Msa0699010 AT5G48140.1 35.207 338 202 7 88 422 34 357 8.76e-59 199
Msa0699010 AT2G15450.1 35.475 358 207 9 74 422 30 372 2.20e-58 198
Msa0699010 AT2G15470.1 35.196 358 208 9 74 422 30 372 6.55e-57 194
Msa0699010 AT4G35670.2 35.185 270 161 3 158 427 2 257 3.76e-53 181
Msa0699010 AT4G32370.1 37.201 293 162 6 89 373 49 327 6.53e-53 182
Msa0699010 AT4G32375.1 36.667 270 149 4 158 422 13 265 2.52e-50 177
Msa0699010 AT4G32380.2 29.730 370 197 8 89 452 41 353 2.64e-46 166
Msa0699010 AT4G33440.1 28.070 342 192 12 48 358 52 370 7.74e-23 101
Msa0699010 AT3G42950.1 26.579 380 234 12 73 422 64 428 1.96e-20 94.4
Msa0699010 AT1G19170.1 25.126 398 238 13 63 422 75 450 9.42e-20 92.4
Msa0699010 AT3G62110.1 26.149 348 211 11 77 393 45 377 1.13e-19 92.0
Msa0699010 AT3G62110.2 26.149 348 211 11 77 393 45 377 1.13e-19 92.0
Msa0699010 AT5G41870.1 26.235 324 188 12 74 364 44 349 5.99e-19 89.4
Msa0699010 AT5G49215.1 25.789 380 228 13 77 422 45 404 2.82e-17 84.3
Msa0699010 AT5G49215.2 25.333 375 236 11 77 422 43 402 3.38e-17 84.3
Msa0699010 AT4G23820.1 25.552 317 192 8 77 364 43 344 4.45e-17 84.0
Msa0699010 AT3G48950.1 23.642 313 193 8 77 358 47 344 1.09e-16 82.8
Msa0699010 AT2G23900.1 26.923 312 184 12 77 358 58 355 1.17e-15 79.7
Msa0699010 AT3G61490.4 24.516 310 193 10 77 358 160 456 2.47e-14 75.9
Msa0699010 AT3G61490.2 24.516 310 193 10 77 358 47 343 2.62e-14 75.5
Msa0699010 AT3G61490.3 24.516 310 193 10 77 358 47 343 2.62e-14 75.5
Msa0699010 AT3G61490.1 24.516 310 193 10 77 358 47 343 2.62e-14 75.5
Msa0699010 AT3G61490.5 24.516 310 193 10 77 358 62 358 2.99e-14 75.1
Msa0699010 AT4G23500.1 26.214 309 177 11 80 355 73 363 7.18e-13 70.9

Find 145 sgRNAs with CRISPR-Local

Find 233 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTCCAATTAATTGTTTA+AGG 0.064758 4_4:+69549919 Msa0699010:CDS
TCTACCTAATCAACCAAATT+TGG 0.144380 4_4:-69550012 None:intergenic
TCTTAGGATTCGTCCCTATT+TGG 0.168680 4_4:+69550119 Msa0699010:three_prime_UTR
TCCTCGGAAATCCTTAATTT+TGG 0.179209 4_4:-69550258 None:intergenic
ATGTTCCTCGGTATTTAATT+TGG 0.184875 4_4:-69550352 None:intergenic
CAGTTCCACATGATATTTAA+TGG 0.196238 4_4:+69548883 Msa0699010:CDS
CCTTCCCTTAAACAATTAAT+TGG 0.200232 4_4:-69549924 None:intergenic
TTCATGGACTATTTATTCTT+TGG 0.220446 4_4:+69547317 Msa0699010:CDS
ATGGGAAAATACACCCAAAT+AGG 0.222592 4_4:-69550133 None:intergenic
TTAATTACCATTGCTTATTA+AGG 0.227433 4_4:-69549013 None:intergenic
TCTCAAATTAAGTAGTATTA+AGG 0.230171 4_4:-69547344 None:intergenic
CCATAAGCATTCCAACAAAA+AGG 0.243640 4_4:-69549876 None:intergenic
ATATTTGTGTTAAAGATAAT+TGG 0.246059 4_4:-69547185 None:intergenic
ATGATGTATTAGTCCAAATT+TGG 0.262749 4_4:+69549999 Msa0699010:three_prime_UTR
CAGCAACTCATCGCTCACTA+TGG 0.305602 4_4:-69550152 None:intergenic
GTTTACCCTGTGAGGTTTGC+AGG 0.305698 4_4:-69548064 None:intergenic
GACGAATCCCGAGGATCATT+TGG 0.309383 4_4:-69547493 None:intergenic
GTTACATCATTTGGAGCAAT+TGG 0.311153 4_4:+69547535 Msa0699010:CDS
CTTAGGATTCGTCCCTATTT+GGG 0.316030 4_4:+69550120 Msa0699010:three_prime_UTR
TAGTCCATTCACATACAAAT+TGG 0.324642 4_4:-69548842 None:intergenic
CTTACTTGAAATACTATTCC+TGG 0.325710 4_4:-69547694 None:intergenic
AACAATAGTATTGGTCTATA+AGG 0.326820 4_4:-69549665 None:intergenic
AACACAAATATTTCTGAAAC+AGG 0.328264 4_4:+69547197 Msa0699010:five_prime_UTR
TAGTCCAAATTTGGTTGATT+AGG 0.339571 4_4:+69550008 Msa0699010:three_prime_UTR
TGTAGAGTCAGGAGTTCTTC+TGG 0.355959 4_4:+69547615 Msa0699010:CDS
TATATATTATATATTACATT+AGG 0.360280 4_4:+69550038 Msa0699010:three_prime_UTR
TTGATCTCCATTTCCTTCAA+TGG 0.361661 4_4:-69548028 None:intergenic
ATGGTTTCTCGCCAAAATTA+AGG 0.369695 4_4:+69550247 Msa0699010:three_prime_UTR
GCGCGCCGCACTGAGTTTGC+AGG 0.375556 4_4:-69550325 None:intergenic
GAAAGATCACCCACAGTCTC+AGG 0.377658 4_4:-69549951 None:intergenic
GGATCATTTGAAGACTTAGA+AGG 0.381774 4_4:-69547424 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.384128 4_4:+69549157 Msa0699010:intron
ACATGGATTCCATCAGTGTT+GGG 0.385395 4_4:-69548958 None:intergenic
CTGTACCATTAAAACTCATT+TGG 0.389055 4_4:-69548002 None:intergenic
ACCTCCAATTAATTGTTTAA+GGG 0.389356 4_4:+69549920 Msa0699010:CDS
TCTGCCTCTGGCCATGAATC+AGG 0.393547 4_4:-69547943 None:intergenic
TGATCCTCGGGATTCGTCTT+CGG 0.398088 4_4:+69547498 Msa0699010:CDS
TCAGTGTTGGGGCTAAGTTT+TGG 0.401262 4_4:-69548946 None:intergenic
TACAACGGTCATACACGCTT+TGG 0.405221 4_4:-69550412 None:intergenic
GGCCATGAATCAGGTCCATC+TGG 0.406021 4_4:-69547934 None:intergenic
TCCCATATGGATCTCATTCA+TGG 0.407523 4_4:+69547301 Msa0699010:exon
GTGGTCCTAGCCACGGGATT+AGG 0.416076 4_4:+69549218 Msa0699010:CDS
TTCCATGCTTTCTTGAATGC+AGG 0.417079 4_4:-69547580 None:intergenic
TCACTACAAGCAAAATGAAT+AGG 0.422913 4_4:-69549780 None:intergenic
TGATTTCTGCTATCTGCCTC+TGG 0.424057 4_4:-69547955 None:intergenic
TGGGAAAATACACCCAAATA+GGG 0.426962 4_4:-69550132 None:intergenic
ACAGGAACCATTGAAGGAAA+TGG 0.436139 4_4:+69548021 Msa0699010:CDS
CCTTTGTCAAACAATAGTAT+TGG 0.440502 4_4:-69549674 None:intergenic
CACCAGATGGACCTGATTCA+TGG 0.440641 4_4:+69547932 Msa0699010:CDS
AGGAACACTAGGAGAAGAGT+TGG 0.444516 4_4:-69547458 None:intergenic
AACATGGATTCCATCAGTGT+TGG 0.447865 4_4:-69548959 None:intergenic
TCCCGAGGATCATTTGGATA+AGG 0.448420 4_4:-69547487 None:intergenic
AATGGTACAGGAACCATTGA+AGG 0.452512 4_4:+69548015 Msa0699010:CDS
CCCTTATCCAAATGATCCTC+GGG 0.453352 4_4:+69547486 Msa0699010:CDS
GTCCATGAATGAGATCCATA+TGG 0.453446 4_4:-69547303 None:intergenic
GCAGCTTGTGCTGTAGAGTC+AGG 0.454931 4_4:+69547604 Msa0699010:CDS
ATCTTCCCTGCAAACCTCAC+AGG 0.456078 4_4:+69548058 Msa0699010:CDS
AAAATTTGAGAACATACAAA+TGG 0.456117 4_4:+69549623 Msa0699010:CDS
TGAGCGATGAGTTGCTGAAT+TGG 0.456799 4_4:+69550158 Msa0699010:three_prime_UTR
GCCATTTGAAGGTGAATTGT+TGG 0.464227 4_4:+69549848 Msa0699010:CDS
TCCCTTAAACAATTAATTGG+AGG 0.466396 4_4:-69549921 None:intergenic
TGGACCTACCACTACAAGAA+AGG 0.466449 4_4:+69550191 Msa0699010:three_prime_UTR
TTTCATATCTATAGATGATA+CGG 0.467936 4_4:+69548806 Msa0699010:intron
AAGACATGGCAAGGTGGAAT+GGG 0.468398 4_4:+69549585 Msa0699010:CDS
TATTTCCACATTTGAAGTCC+CGG 0.469866 4_4:-69549182 None:intergenic
CCCGAGGATCATTTGGATAA+GGG 0.470657 4_4:-69547486 None:intergenic
GTTGAAAACTCACAAGATGT+TGG 0.482732 4_4:+69548979 Msa0699010:CDS
TAAGTTTGAGACACATGCTT+GGG 0.483234 4_4:-69549514 None:intergenic
TTGAAAACTCACAAGATGTT+GGG 0.483533 4_4:+69548980 Msa0699010:CDS
TGAAATTGAAGAAAGAAGAA+TGG 0.492537 4_4:-69547155 None:intergenic
AATACTATTCCTGGTTTGCA+TGG 0.493977 4_4:-69547685 None:intergenic
TCAGGTGGATGGTATGCTAA+TGG 0.494463 4_4:+69547909 Msa0699010:intron
AGCTCCAATTTGTATGTGAA+TGG 0.494817 4_4:+69548838 Msa0699010:CDS
CCAATTAATTGTTTAAGGGA+AGG 0.495839 4_4:+69549924 Msa0699010:CDS
TTGTACAGCATAGGAAGTCT+TGG 0.499986 4_4:+69549480 Msa0699010:intron
AAAGACATGGCAAGGTGGAA+TGG 0.501829 4_4:+69549584 Msa0699010:CDS
ACCCTTATCCAAATGATCCT+CGG 0.501972 4_4:+69547485 Msa0699010:CDS
GGCGAGAAACCATTGTTCCT+CGG 0.502286 4_4:-69550237 None:intergenic
TCCAACAATTCACCTTCAAA+TGG 0.505935 4_4:-69549849 None:intergenic
ACATATTGATCATTACTCCT+TGG 0.515439 4_4:-69548909 None:intergenic
ACTACTTAATTTGAGAAATG+TGG 0.520711 4_4:+69547351 Msa0699010:CDS
TAACTTGTGGTCCTAGCCAC+GGG 0.522097 4_4:+69549212 Msa0699010:CDS
ACTTCAAATGTGGAAATAGC+TGG 0.529833 4_4:+69549187 Msa0699010:CDS
AGCAACTCATCGCTCACTAT+GGG 0.537725 4_4:-69550151 None:intergenic
TCATTTGGAGCAATTGGAGA+TGG 0.537985 4_4:+69547541 Msa0699010:CDS
GTAACTTGTGGTCCTAGCCA+CGG 0.538941 4_4:+69549211 Msa0699010:CDS
ATTTGAGAAATGTGGATGGA+AGG 0.539093 4_4:+69547359 Msa0699010:CDS
ATGATATTTAATGGCTGCCA+AGG 0.542871 4_4:+69548892 Msa0699010:CDS
AACATACAAATGGAGAATGT+AGG 0.544153 4_4:+69549633 Msa0699010:CDS
ATTTCCACATTTGAAGTCCC+GGG 0.546166 4_4:-69549181 None:intergenic
AAGGAAATGGAGATCAATGG+TGG 0.551216 4_4:+69548034 Msa0699010:CDS
TTAAGTTTGAGACACATGCT+TGG 0.553610 4_4:-69549515 None:intergenic
AAACTTAGCCCCAACACTGA+TGG 0.554244 4_4:+69548949 Msa0699010:CDS
GATTGCATTTCAATTGGACC+CGG 0.555852 4_4:+69549163 Msa0699010:CDS
TACAACTCCTTAATAAGCAA+TGG 0.557489 4_4:+69549006 Msa0699010:CDS
TTGAAGACTTAGAAGGAACA+AGG 0.561772 4_4:-69547417 None:intergenic
CACCTGCATTCAAGAAAGCA+TGG 0.563512 4_4:+69547578 Msa0699010:CDS
TTGAAATGTAATCAGGTGGA+TGG 0.567449 4_4:+69547898 Msa0699010:intron
CTTAATTTGAGAAATGTGGA+TGG 0.570049 4_4:+69547355 Msa0699010:CDS
TTGAAGGAAATGGAGATCAA+TGG 0.574015 4_4:+69548031 Msa0699010:CDS
ATACACAACCTTTCTTGTAG+TGG 0.574452 4_4:-69550199 None:intergenic
TGAAGAAAGAAGAATGGCTA+AGG 0.575307 4_4:-69547149 None:intergenic
TTTACCCTGTGAGGTTTGCA+GGG 0.576342 4_4:-69548063 None:intergenic
TCATTTGGATAAGGGTCAGA+AGG 0.578630 4_4:-69547478 None:intergenic
CGCCGCACTGAGTTTGCAGG+AGG 0.586427 4_4:-69550322 None:intergenic
ACAACCTTTCTTGTAGTGGT+AGG 0.592005 4_4:-69550195 None:intergenic
ATGTACCTAATCCCGTGGCT+AGG 0.592436 4_4:-69549223 None:intergenic
CCAATACTATTGTTTGACAA+AGG 0.596001 4_4:+69549674 Msa0699010:CDS
GGAAGGTGACCCTGAGACTG+TGG 0.598346 4_4:+69549941 Msa0699010:CDS
TTAAATATCATGTGGAACTG+AGG 0.598983 4_4:-69548880 None:intergenic
TTTCCGAGGAACATGTTCCT+CGG 0.599886 4_4:-69550364 None:intergenic
TCTTCCCTGCAAACCTCACA+GGG 0.600082 4_4:+69548059 Msa0699010:CDS
AGGCAGATAGCAGAAATCAA+TGG 0.605028 4_4:+69547959 Msa0699010:CDS
TGGACCCGGGACTTCAAATG+TGG 0.605054 4_4:+69549177 Msa0699010:CDS
CTCACAGCTGGACTTCCACA+TGG 0.605942 4_4:-69548319 None:intergenic
TCCATGAATGAGATCCATAT+GGG 0.606027 4_4:-69547302 None:intergenic
ATACCGAGGAACATGTTCCT+CGG 0.606054 4_4:+69550361 Msa0699010:three_prime_UTR
TCTTATGACTGTGTCACGGA+CGG 0.610881 4_4:-69549538 None:intergenic
CTAAGAATAAAGACATGGCA+AGG 0.613229 4_4:+69549576 Msa0699010:CDS
GGTATGCTAATGGCACCAGA+TGG 0.619104 4_4:+69547919 Msa0699010:CDS
GCCAAAATTAAGGATTTCCG+AGG 0.620966 4_4:+69550257 Msa0699010:three_prime_UTR
GGCAGCCATTAAATATCATG+TGG 0.622918 4_4:-69548888 None:intergenic
CATGGATTCCATCAGTGTTG+GGG 0.624775 4_4:-69548957 None:intergenic
TAAGGGTCAGAAGGAACACT+AGG 0.625203 4_4:-69547469 None:intergenic
GCACTGAGTTTGCAGGAGGG+AGG 0.628274 4_4:-69550318 None:intergenic
ATTGCATTTCAATTGGACCC+GGG 0.630537 4_4:+69549164 Msa0699010:CDS
TGAAGACTTAGAAGGAACAA+GGG 0.632788 4_4:-69547416 None:intergenic
ACACTAGGAGAAGAGTTGGA+AGG 0.640153 4_4:-69547454 None:intergenic
AAAGATCACCCACAGTCTCA+GGG 0.643345 4_4:-69549950 None:intergenic
ATGTAATGTACCTAATCCCG+TGG 0.645529 4_4:-69549228 None:intergenic
GCCGCACTGAGTTTGCAGGA+GGG 0.648337 4_4:-69550321 None:intergenic
TGGACCTGATTCATGGCCAG+AGG 0.650317 4_4:+69547939 Msa0699010:CDS
GAAATAGCTGGTGTAACTTG+TGG 0.654498 4_4:+69549199 Msa0699010:CDS
GTCATAAGAGAATCAGACAA+TGG 0.654639 4_4:+69549552 Msa0699010:CDS
TCTTGTTTAATACATTACAA+CGG 0.655174 4_4:-69550427 None:intergenic
ATTCTCTTATGACTGTGTCA+CGG 0.659505 4_4:-69549542 None:intergenic
AAAACTTTGTCAGGACCATG+TGG 0.660723 4_4:+69548304 Msa0699010:CDS
AGGAAATGGAGATCAATGGT+GGG 0.664389 4_4:+69548035 Msa0699010:CDS
TACAAACATAACACTCTCTG+AGG 0.682981 4_4:+69549815 Msa0699010:CDS
ATTCTGACTTGTACATACCG+AGG 0.689097 4_4:-69550288 None:intergenic
AGAATAAAGACATGGCAAGG+TGG 0.690276 4_4:+69549579 Msa0699010:CDS
TCCCTCCTGCAAACTCAGTG+CGG 0.694919 4_4:+69550320 Msa0699010:three_prime_UTR
CAGTCCGAAGACGAATCCCG+AGG 0.710358 4_4:-69547502 None:intergenic
ATGGACTAAGAATAAAGACA+TGG 0.715833 4_4:+69549571 Msa0699010:CDS
GAAGGTGACCCTGAGACTGT+GGG 0.722175 4_4:+69549942 Msa0699010:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAAATAAATGATATATA+TGG - chr4_4:69547749-69547768 None:intergenic 10.0%
!! TCTATAAATAAATATTTACA+TGG - chr4_4:69548119-69548138 None:intergenic 10.0%
!! ATTCTATATGTAATTAATAT+AGG + chr4_4:69548372-69548391 Msa0699010:intron 10.0%
!! TTCTATATGTAATTAATATA+GGG + chr4_4:69548373-69548392 Msa0699010:intron 10.0%
!!! ATATCCTATTTTTATTTTTA+AGG + chr4_4:69548686-69548705 Msa0699010:intron 10.0%
!!! TTAAAATTCGTTTTATTTTT+AGG + chr4_4:69548716-69548735 Msa0699010:intron 10.0%
!!! TAAAATTCGTTTTATTTTTA+GGG + chr4_4:69548717-69548736 Msa0699010:intron 10.0%
!!! ATATTTTGAATAGAAAAATT+AGG - chr4_4:69549335-69549354 None:intergenic 10.0%
!! ATATTTGTGTTAAAGATAAT+TGG - chr4_4:69547188-69547207 None:intergenic 15.0%
!! TCAACTTAATAAAATATGAT+TGG + chr4_4:69547803-69547822 Msa0699010:intron 15.0%
!!! AAAATTTTCCATTTTGTTTT+TGG + chr4_4:69548481-69548500 Msa0699010:intron 15.0%
!! ATGTCAAAATTATATCAATT+AGG + chr4_4:69548627-69548646 Msa0699010:intron 15.0%
!! TGTCAAAATTATATCAATTA+GGG + chr4_4:69548628-69548647 Msa0699010:intron 15.0%
!! TAGTCCTTAAAAATAAAAAT+AGG - chr4_4:69548693-69548712 None:intergenic 15.0%
!!! AAAATTCGTTTTATTTTTAG+GGG + chr4_4:69548718-69548737 Msa0699010:intron 15.0%
!!! ATTATATGTTTTGATTGTTA+TGG + chr4_4:69550079-69550098 Msa0699010:three_prime_UTR 15.0%
!!! TAAACAAGAATTTTATATAG+AGG + chr4_4:69550441-69550460 Msa0699010:three_prime_UTR 15.0%
!! TCTCAAATTAAGTAGTATTA+AGG - chr4_4:69547347-69547366 None:intergenic 20.0%
!!! ATCATAAAAGTGTAATTTTC+TGG - chr4_4:69547643-69547662 None:intergenic 20.0%
!!! ATCACTTCAATTATTTTTTC+AGG + chr4_4:69547661-69547680 Msa0699010:CDS 20.0%
!! AACAAAATTGAAATGTAATC+AGG + chr4_4:69547891-69547910 Msa0699010:intron 20.0%
!!! ATAGTGTAATTTGTTTTACA+CGG + chr4_4:69548173-69548192 Msa0699010:intron 20.0%
!!! AAAATGCCAAGTTTTATTTT+TGG - chr4_4:69548269-69548288 None:intergenic 20.0%
!!! ACATCTCGTTTTTATTTTTA+TGG - chr4_4:69548449-69548468 None:intergenic 20.0%
!!! ACAAAATGGAAAATTTTGAT+AGG - chr4_4:69548478-69548497 None:intergenic 20.0%
!! TAAACATAAATTGGTCATTA+GGG - chr4_4:69548508-69548527 None:intergenic 20.0%
!! ATAAACATAAATTGGTCATT+AGG - chr4_4:69548509-69548528 None:intergenic 20.0%
!! ACATTAGCATAAACATAAAT+TGG - chr4_4:69548517-69548536 None:intergenic 20.0%
!! GACATTTCTTTAATATAACA+TGG - chr4_4:69548601-69548620 None:intergenic 20.0%
!!! AAATGAAACAAAAATTTTCG+AGG + chr4_4:69548656-69548675 Msa0699010:intron 20.0%
!!! AATGAAACAAAAATTTTCGA+GGG + chr4_4:69548657-69548676 Msa0699010:intron 20.0%
!! TTTCATATCTATAGATGATA+CGG + chr4_4:69548806-69548825 Msa0699010:intron 20.0%
!! TTAATTACCATTGCTTATTA+AGG - chr4_4:69549016-69549035 None:intergenic 20.0%
!! TAATAAGCAATGGTAATTAA+TGG + chr4_4:69549016-69549035 Msa0699010:intron 20.0%
!! AACATAGAAACAATAAAACT+GGG - chr4_4:69549067-69549086 None:intergenic 20.0%
!! TAACATAGAAACAATAAAAC+TGG - chr4_4:69549068-69549087 None:intergenic 20.0%
!! TTACTATTGACAAAATTTAG+AGG + chr4_4:69549354-69549373 Msa0699010:intron 20.0%
!! AAAATTTGAGAACATACAAA+TGG + chr4_4:69549623-69549642 Msa0699010:CDS 20.0%
!!! TCTTGTTTAATACATTACAA+CGG - chr4_4:69550430-69550449 None:intergenic 20.0%
! TGAAATTGAAGAAAGAAGAA+TGG - chr4_4:69547158-69547177 None:intergenic 25.0%
!!! AACACAAATATTTCTGAAAC+AGG + chr4_4:69547197-69547216 Msa0699010:five_prime_UTR 25.0%
!!! ATTTTGTTGTAGCACAATAT+TGG + chr4_4:69547259-69547278 Msa0699010:five_prime_UTR 25.0%
!! TTCATGGACTATTTATTCTT+TGG + chr4_4:69547317-69547336 Msa0699010:CDS 25.0%
!! ACTACTTAATTTGAGAAATG+TGG + chr4_4:69547351-69547370 Msa0699010:CDS 25.0%
!! ATTTTCGATGTTACATCATT+TGG + chr4_4:69547526-69547545 Msa0699010:CDS 25.0%
! AAAATTGAAATGTAATCAGG+TGG + chr4_4:69547894-69547913 Msa0699010:intron 25.0%
!!! CAAGTTTTATTTTTGGTTAG+TGG - chr4_4:69548262-69548281 None:intergenic 25.0%
! CACTAACCAAAAATAAAACT+TGG + chr4_4:69548260-69548279 Msa0699010:intron 25.0%
!! AAATAAAACTTGGCATTTTC+AGG + chr4_4:69548270-69548289 Msa0699010:intron 25.0%
!! AATAAAACTTGGCATTTTCA+GGG + chr4_4:69548271-69548290 Msa0699010:intron 25.0%
! TTTAATATAACATGGACTCA+GGG - chr4_4:69548593-69548612 None:intergenic 25.0%
! CTGGGTAAGTAAATTAATAT+TGG - chr4_4:69549049-69549068 None:intergenic 25.0%
!!! AACATGATTTGATTTTGAAC+AGG + chr4_4:69549136-69549155 Msa0699010:intron 25.0%
! TGAATAGAAAAATTAGGATC+TGG - chr4_4:69549329-69549348 None:intergenic 25.0%
! TTAGAGGTGAAATATGAAAT+CGG + chr4_4:69549370-69549389 Msa0699010:intron 25.0%
!! TTGTTAATTTTGTACAGCAT+AGG + chr4_4:69549471-69549490 Msa0699010:intron 25.0%
!!! AACAATAGTATTGGTCTATA+AGG - chr4_4:69549668-69549687 None:intergenic 25.0%
! TACCTCCAATTAATTGTTTA+AGG + chr4_4:69549919-69549938 Msa0699010:CDS 25.0%
! ACCTCCAATTAATTGTTTAA+GGG + chr4_4:69549920-69549939 Msa0699010:CDS 25.0%
! ATGATGTATTAGTCCAAATT+TGG + chr4_4:69549999-69550018 Msa0699010:three_prime_UTR 25.0%
!!! TTTTTTTATGTGTGCATCTT+AGG + chr4_4:69550103-69550122 Msa0699010:three_prime_UTR 25.0%
! CAAATTTTGAACTTCCCATA+TGG + chr4_4:69547288-69547307 Msa0699010:exon 30.0%
! CTTAATTTGAGAAATGTGGA+TGG + chr4_4:69547355-69547374 Msa0699010:CDS 30.0%
CTTACTTGAAATACTATTCC+TGG - chr4_4:69547697-69547716 None:intergenic 30.0%
!! CTTGACCAAATGAGTTTTAA+TGG + chr4_4:69547997-69548016 Msa0699010:CDS 30.0%
CTGTACCATTAAAACTCATT+TGG - chr4_4:69548005-69548024 None:intergenic 30.0%
!! CAAATGAGTTTTAATGGTAC+AGG + chr4_4:69548003-69548022 Msa0699010:CDS 30.0%
TATTTAATGACTGAGCTGAA+TGG - chr4_4:69548350-69548369 None:intergenic 30.0%
ATTAGGGACCAAAAACAAAA+TGG - chr4_4:69548492-69548511 None:intergenic 30.0%
TTAATATAACATGGACTCAG+GGG - chr4_4:69548592-69548611 None:intergenic 30.0%
CTTTAATATAACATGGACTC+AGG - chr4_4:69548594-69548613 None:intergenic 30.0%
TAGTCCATTCACATACAAAT+TGG - chr4_4:69548845-69548864 None:intergenic 30.0%
TTAAATATCATGTGGAACTG+AGG - chr4_4:69548883-69548902 None:intergenic 30.0%
CAGTTCCACATGATATTTAA+TGG + chr4_4:69548883-69548902 Msa0699010:CDS 30.0%
ACATATTGATCATTACTCCT+TGG - chr4_4:69548912-69548931 None:intergenic 30.0%
TTGAAAACTCACAAGATGTT+GGG + chr4_4:69548980-69548999 Msa0699010:CDS 30.0%
TACAACTCCTTAATAAGCAA+TGG + chr4_4:69549006-69549025 Msa0699010:CDS 30.0%
GCTGCTTGCTTATATAAAAA+TGG - chr4_4:69549106-69549125 None:intergenic 30.0%
ACTAAACTTCGTGACAAAAT+TGG - chr4_4:69549399-69549418 None:intergenic 30.0%
! ATGGACTAAGAATAAAGACA+TGG + chr4_4:69549571-69549590 Msa0699010:CDS 30.0%
AACATACAAATGGAGAATGT+AGG + chr4_4:69549633-69549652 Msa0699010:CDS 30.0%
CCTTTGTCAAACAATAGTAT+TGG - chr4_4:69549677-69549696 None:intergenic 30.0%
CCAATACTATTGTTTGACAA+AGG + chr4_4:69549674-69549693 Msa0699010:CDS 30.0%
TGTGTCCTACAAAAACATTA+AGG + chr4_4:69549734-69549753 Msa0699010:CDS 30.0%
GTGTCCTACAAAAACATTAA+GGG + chr4_4:69549735-69549754 Msa0699010:CDS 30.0%
!! TGTACCCTTAATGTTTTTGT+AGG - chr4_4:69549742-69549761 None:intergenic 30.0%
TCACTACAAGCAAAATGAAT+AGG - chr4_4:69549783-69549802 None:intergenic 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr4_4:69549878-69549897 None:intergenic 30.0%
TCCCTTAAACAATTAATTGG+AGG - chr4_4:69549924-69549943 None:intergenic 30.0%
CCTTCCCTTAAACAATTAAT+TGG - chr4_4:69549927-69549946 None:intergenic 30.0%
CCAATTAATTGTTTAAGGGA+AGG + chr4_4:69549924-69549943 Msa0699010:CDS 30.0%
TAGTCCAAATTTGGTTGATT+AGG + chr4_4:69550008-69550027 Msa0699010:three_prime_UTR 30.0%
TCTACCTAATCAACCAAATT+TGG - chr4_4:69550015-69550034 None:intergenic 30.0%
!!! GCTGAATTGGTTCATTTTTT+TGG + chr4_4:69550171-69550190 Msa0699010:three_prime_UTR 30.0%
! CTTTTCCAAATTAAATACCG+AGG + chr4_4:69550347-69550366 Msa0699010:three_prime_UTR 30.0%
ATGTTCCTCGGTATTTAATT+TGG - chr4_4:69550355-69550374 None:intergenic 30.0%
TGAAGAAAGAAGAATGGCTA+AGG - chr4_4:69547152-69547171 None:intergenic 35.0%
TCCATGAATGAGATCCATAT+GGG - chr4_4:69547305-69547324 None:intergenic 35.0%
ATTTGAGAAATGTGGATGGA+AGG + chr4_4:69547359-69547378 Msa0699010:CDS 35.0%
! GGAAGATACTTTCTTTTGCT+TGG - chr4_4:69547398-69547417 None:intergenic 35.0%
TGAAGACTTAGAAGGAACAA+GGG - chr4_4:69547419-69547438 None:intergenic 35.0%
TTGAAGACTTAGAAGGAACA+AGG - chr4_4:69547420-69547439 None:intergenic 35.0%
GGATCATTTGAAGACTTAGA+AGG - chr4_4:69547427-69547446 None:intergenic 35.0%
! GTTACATCATTTGGAGCAAT+TGG + chr4_4:69547535-69547554 Msa0699010:CDS 35.0%
! AATACTATTCCTGGTTTGCA+TGG - chr4_4:69547688-69547707 None:intergenic 35.0%
ATTGGACGTAAGTGTAAAAC+TGG + chr4_4:69547821-69547840 Msa0699010:intron 35.0%
TTGAAATGTAATCAGGTGGA+TGG + chr4_4:69547898-69547917 Msa0699010:intron 35.0%
TTGATCTCCATTTCCTTCAA+TGG - chr4_4:69548031-69548050 None:intergenic 35.0%
TTGAAGGAAATGGAGATCAA+TGG + chr4_4:69548031-69548050 Msa0699010:CDS 35.0%
TCAAAAAAGTTTACCCTGTG+AGG - chr4_4:69548075-69548094 None:intergenic 35.0%
TAATATAACATGGACTCAGG+GGG - chr4_4:69548591-69548610 None:intergenic 35.0%
AGCTCCAATTTGTATGTGAA+TGG + chr4_4:69548838-69548857 Msa0699010:CDS 35.0%
ATGATATTTAATGGCTGCCA+AGG + chr4_4:69548892-69548911 Msa0699010:CDS 35.0%
! CATCTTGTGAGTTTTCAACA+TGG - chr4_4:69548978-69548997 None:intergenic 35.0%
GTTGAAAACTCACAAGATGT+TGG + chr4_4:69548979-69548998 Msa0699010:CDS 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr4_4:69549157-69549176 Msa0699010:intron 35.0%
! TATTTCCACATTTGAAGTCC+CGG - chr4_4:69549185-69549204 None:intergenic 35.0%
ACTTCAAATGTGGAAATAGC+TGG + chr4_4:69549187-69549206 Msa0699010:CDS 35.0%
! TAAGTTTGAGACACATGCTT+GGG - chr4_4:69549517-69549536 None:intergenic 35.0%
! TTAAGTTTGAGACACATGCT+TGG - chr4_4:69549518-69549537 None:intergenic 35.0%
ATTCTCTTATGACTGTGTCA+CGG - chr4_4:69549545-69549564 None:intergenic 35.0%
GTCATAAGAGAATCAGACAA+TGG + chr4_4:69549552-69549571 Msa0699010:CDS 35.0%
CTAAGAATAAAGACATGGCA+AGG + chr4_4:69549576-69549595 Msa0699010:CDS 35.0%
TACAAACATAACACTCTCTG+AGG + chr4_4:69549815-69549834 Msa0699010:CDS 35.0%
TCCAACAATTCACCTTCAAA+TGG - chr4_4:69549852-69549871 None:intergenic 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr4_4:69549879-69549898 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr4_4:69549876-69549895 Msa0699010:CDS 35.0%
TGGGAAAATACACCCAAATA+GGG - chr4_4:69550135-69550154 None:intergenic 35.0%
ATGGGAAAATACACCCAAAT+AGG - chr4_4:69550136-69550155 None:intergenic 35.0%
ATACACAACCTTTCTTGTAG+TGG - chr4_4:69550202-69550221 None:intergenic 35.0%
! CATTGTTCCTCGGAAATTTT+TGG - chr4_4:69550230-69550249 None:intergenic 35.0%
CAAAAATTTCCGAGGAACAA+TGG + chr4_4:69550228-69550247 Msa0699010:three_prime_UTR 35.0%
ATGGTTTCTCGCCAAAATTA+AGG + chr4_4:69550247-69550266 Msa0699010:three_prime_UTR 35.0%
! TCCTCGGAAATCCTTAATTT+TGG - chr4_4:69550261-69550280 None:intergenic 35.0%
! TAATGACAACATGTTTTCCG+AGG - chr4_4:69550381-69550400 None:intergenic 35.0%
TCCCATATGGATCTCATTCA+TGG + chr4_4:69547301-69547320 Msa0699010:exon 40.0%
GTCCATGAATGAGATCCATA+TGG - chr4_4:69547306-69547325 None:intergenic 40.0%
!!! TCTTTTGCTTGGTGTGAAAG+TGG - chr4_4:69547387-69547406 None:intergenic 40.0%
! GAAGAGTTGGAAGGATTTTC+TGG - chr4_4:69547448-69547467 None:intergenic 40.0%
TCATTTGGATAAGGGTCAGA+AGG - chr4_4:69547481-69547500 None:intergenic 40.0%
ACCCTTATCCAAATGATCCT+CGG + chr4_4:69547485-69547504 Msa0699010:CDS 40.0%
! TCATTTGGAGCAATTGGAGA+TGG + chr4_4:69547541-69547560 Msa0699010:CDS 40.0%
TTCCATGCTTTCTTGAATGC+AGG - chr4_4:69547583-69547602 None:intergenic 40.0%
AGGCAGATAGCAGAAATCAA+TGG + chr4_4:69547959-69547978 Msa0699010:CDS 40.0%
AATGGTACAGGAACCATTGA+AGG + chr4_4:69548015-69548034 Msa0699010:CDS 40.0%
ACAGGAACCATTGAAGGAAA+TGG + chr4_4:69548021-69548040 Msa0699010:CDS 40.0%
AAGGAAATGGAGATCAATGG+TGG + chr4_4:69548034-69548053 Msa0699010:CDS 40.0%
AGGAAATGGAGATCAATGGT+GGG + chr4_4:69548035-69548054 Msa0699010:CDS 40.0%
! CCTGACAAAGTTTTTCCATC+TGG - chr4_4:69548298-69548317 None:intergenic 40.0%
CCAGATGGAAAAACTTTGTC+AGG + chr4_4:69548295-69548314 Msa0699010:CDS 40.0%
AAAACTTTGTCAGGACCATG+TGG + chr4_4:69548304-69548323 Msa0699010:CDS 40.0%
!! TGAATGGTTTTACTCACAGC+TGG - chr4_4:69548334-69548353 None:intergenic 40.0%
GGCAGCCATTAAATATCATG+TGG - chr4_4:69548891-69548910 None:intergenic 40.0%
!! GCTAAGTTTTGGTGCAGAAA+TGG - chr4_4:69548938-69548957 None:intergenic 40.0%
ACATGGATTCCATCAGTGTT+GGG - chr4_4:69548961-69548980 None:intergenic 40.0%
AACATGGATTCCATCAGTGT+TGG - chr4_4:69548962-69548981 None:intergenic 40.0%
GATTGCATTTCAATTGGACC+CGG + chr4_4:69549163-69549182 Msa0699010:CDS 40.0%
ATTGCATTTCAATTGGACCC+GGG + chr4_4:69549164-69549183 Msa0699010:CDS 40.0%
! ATTTCCACATTTGAAGTCCC+GGG - chr4_4:69549184-69549203 None:intergenic 40.0%
GAAATAGCTGGTGTAACTTG+TGG + chr4_4:69549199-69549218 Msa0699010:CDS 40.0%
ATGTAATGTACCTAATCCCG+TGG - chr4_4:69549231-69549250 None:intergenic 40.0%
CACTACCAAGTGAAACAACA+TGG + chr4_4:69549291-69549310 Msa0699010:intron 40.0%
!! GCAAACCATGTTGTTTCACT+TGG - chr4_4:69549299-69549318 None:intergenic 40.0%
TTGTACAGCATAGGAAGTCT+TGG + chr4_4:69549480-69549499 Msa0699010:intron 40.0%
AGAATAAAGACATGGCAAGG+TGG + chr4_4:69549579-69549598 Msa0699010:CDS 40.0%
!! GTTGAGCTTTTGCCATTTGA+AGG + chr4_4:69549837-69549856 Msa0699010:CDS 40.0%
GCCATTTGAAGGTGAATTGT+TGG + chr4_4:69549848-69549867 Msa0699010:CDS 40.0%
! TGTTGGATGACCCTTTTTGT+TGG + chr4_4:69549865-69549884 Msa0699010:CDS 40.0%
TCTTAGGATTCGTCCCTATT+TGG + chr4_4:69550119-69550138 Msa0699010:three_prime_UTR 40.0%
CTTAGGATTCGTCCCTATTT+GGG + chr4_4:69550120-69550139 Msa0699010:three_prime_UTR 40.0%
ACAACCTTTCTTGTAGTGGT+AGG - chr4_4:69550198-69550217 None:intergenic 40.0%
ATTCCGACCAAAAATTTCCG+AGG + chr4_4:69550220-69550239 Msa0699010:three_prime_UTR 40.0%
!!! GTTCCTCGGAAATTTTTGGT+CGG - chr4_4:69550226-69550245 None:intergenic 40.0%
GCCAAAATTAAGGATTTCCG+AGG + chr4_4:69550257-69550276 Msa0699010:three_prime_UTR 40.0%
!! TTTCCGAGGAACTTTTTCCT+CGG + chr4_4:69550271-69550290 Msa0699010:three_prime_UTR 40.0%
ATACCGAGGAAAAAGTTCCT+CGG - chr4_4:69550277-69550296 None:intergenic 40.0%
! ATTCTGACTTGTACATACCG+AGG - chr4_4:69550291-69550310 None:intergenic 40.0%
ACACTAGGAGAAGAGTTGGA+AGG - chr4_4:69547457-69547476 None:intergenic 45.0%
AGGAACACTAGGAGAAGAGT+TGG - chr4_4:69547461-69547480 None:intergenic 45.0%
TAAGGGTCAGAAGGAACACT+AGG - chr4_4:69547472-69547491 None:intergenic 45.0%
CCCGAGGATCATTTGGATAA+GGG - chr4_4:69547489-69547508 None:intergenic 45.0%
CCCTTATCCAAATGATCCTC+GGG + chr4_4:69547486-69547505 Msa0699010:CDS 45.0%
TCCCGAGGATCATTTGGATA+AGG - chr4_4:69547490-69547509 None:intergenic 45.0%
CACCTGCATTCAAGAAAGCA+TGG + chr4_4:69547578-69547597 Msa0699010:CDS 45.0%
TGTAGAGTCAGGAGTTCTTC+TGG + chr4_4:69547615-69547634 Msa0699010:CDS 45.0%
!!! TTTTCAGGTCCATGCAAACC+AGG + chr4_4:69547676-69547695 Msa0699010:CDS 45.0%
TCAGGTGGATGGTATGCTAA+TGG + chr4_4:69547909-69547928 Msa0699010:intron 45.0%
TGATTTCTGCTATCTGCCTC+TGG - chr4_4:69547958-69547977 None:intergenic 45.0%
TTTACCCTGTGAGGTTTGCA+GGG - chr4_4:69548066-69548085 None:intergenic 45.0%
!! TCAGTGTTGGGGCTAAGTTT+TGG - chr4_4:69548949-69548968 None:intergenic 45.0%
AAACTTAGCCCCAACACTGA+TGG + chr4_4:69548949-69548968 Msa0699010:CDS 45.0%
CATGGATTCCATCAGTGTTG+GGG - chr4_4:69548960-69548979 None:intergenic 45.0%
TCTTATGACTGTGTCACGGA+CGG - chr4_4:69549541-69549560 None:intergenic 45.0%
AAAGACATGGCAAGGTGGAA+TGG + chr4_4:69549584-69549603 Msa0699010:CDS 45.0%
AAGACATGGCAAGGTGGAAT+GGG + chr4_4:69549585-69549604 Msa0699010:CDS 45.0%
AAAGATCACCCACAGTCTCA+GGG - chr4_4:69549953-69549972 None:intergenic 45.0%
AGCAACTCATCGCTCACTAT+GGG - chr4_4:69550154-69550173 None:intergenic 45.0%
TGAGCGATGAGTTGCTGAAT+TGG + chr4_4:69550158-69550177 Msa0699010:three_prime_UTR 45.0%
TGGACCTACCACTACAAGAA+AGG + chr4_4:69550191-69550210 Msa0699010:three_prime_UTR 45.0%
ATACCGAGGAACATGTTCCT+CGG + chr4_4:69550361-69550380 Msa0699010:three_prime_UTR 45.0%
TTTCCGAGGAACATGTTCCT+CGG - chr4_4:69550367-69550386 None:intergenic 45.0%
TACAACGGTCATACACGCTT+TGG - chr4_4:69550415-69550434 None:intergenic 45.0%
!! TATATATTATATATTACATT+AGG + chr4_4:69550038-69550057 Msa0699010:three_prime_UTR 5.0%
GACGAATCCCGAGGATCATT+TGG - chr4_4:69547496-69547515 None:intergenic 50.0%
TGATCCTCGGGATTCGTCTT+CGG + chr4_4:69547498-69547517 Msa0699010:CDS 50.0%
GGTATGCTAATGGCACCAGA+TGG + chr4_4:69547919-69547938 Msa0699010:CDS 50.0%
CACCAGATGGACCTGATTCA+TGG + chr4_4:69547932-69547951 Msa0699010:CDS 50.0%
ATCTTCCCTGCAAACCTCAC+AGG + chr4_4:69548058-69548077 Msa0699010:CDS 50.0%
TCTTCCCTGCAAACCTCACA+GGG + chr4_4:69548059-69548078 Msa0699010:CDS 50.0%
GTTTACCCTGTGAGGTTTGC+AGG - chr4_4:69548067-69548086 None:intergenic 50.0%
!! TGGCATTTTCAGGGTCCAGA+TGG + chr4_4:69548280-69548299 Msa0699010:intron 50.0%
TATAACATGGACTCAGGGGG+TGG - chr4_4:69548588-69548607 None:intergenic 50.0%
! GTAACTTGTGGTCCTAGCCA+CGG + chr4_4:69549211-69549230 Msa0699010:CDS 50.0%
! TAACTTGTGGTCCTAGCCAC+GGG + chr4_4:69549212-69549231 Msa0699010:CDS 50.0%
ATGTACCTAATCCCGTGGCT+AGG - chr4_4:69549226-69549245 None:intergenic 50.0%
GAAAGATCACCCACAGTCTC+AGG - chr4_4:69549954-69549973 None:intergenic 50.0%
CAGCAACTCATCGCTCACTA+TGG - chr4_4:69550155-69550174 None:intergenic 50.0%
GGCGAGAAACCATTGTTCCT+CGG - chr4_4:69550240-69550259 None:intergenic 50.0%
GCAGCTTGTGCTGTAGAGTC+AGG + chr4_4:69547604-69547623 Msa0699010:CDS 55.0%
GGCCATGAATCAGGTCCATC+TGG - chr4_4:69547937-69547956 None:intergenic 55.0%
TGGACCTGATTCATGGCCAG+AGG + chr4_4:69547939-69547958 Msa0699010:CDS 55.0%
TCTGCCTCTGGCCATGAATC+AGG - chr4_4:69547946-69547965 None:intergenic 55.0%
CTCACAGCTGGACTTCCACA+TGG - chr4_4:69548322-69548341 None:intergenic 55.0%
TGGACCCGGGACTTCAAATG+TGG + chr4_4:69549177-69549196 Msa0699010:CDS 55.0%
GAAGGTGACCCTGAGACTGT+GGG + chr4_4:69549942-69549961 Msa0699010:CDS 55.0%
TCCCTCCTGCAAACTCAGTG+CGG + chr4_4:69550320-69550339 Msa0699010:three_prime_UTR 55.0%
CAGTCCGAAGACGAATCCCG+AGG - chr4_4:69547505-69547524 None:intergenic 60.0%
GTGGTCCTAGCCACGGGATT+AGG + chr4_4:69549218-69549237 Msa0699010:CDS 60.0%
GGAAGGTGACCCTGAGACTG+TGG + chr4_4:69549941-69549960 Msa0699010:CDS 60.0%
GCACTGAGTTTGCAGGAGGG+AGG - chr4_4:69550321-69550340 None:intergenic 60.0%
GCCGCACTGAGTTTGCAGGA+GGG - chr4_4:69550324-69550343 None:intergenic 60.0%
CGCCGCACTGAGTTTGCAGG+AGG - chr4_4:69550325-69550344 None:intergenic 65.0%
GCGCGCCGCACTGAGTTTGC+AGG - chr4_4:69550328-69550347 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 69547134 69550477 69547134 ID=Msa0699010;Name=Msa0699010
chr4_4 mRNA 69547134 69550477 69547134 ID=Msa0699010-mRNA-1;Parent=Msa0699010;Name=Msa0699010-mRNA-1;_AED=0.11;_eAED=0.11;_QI=173|1|1|1|1|1|6|482|471
chr4_4 exon 69547134 69547711 69547134 ID=Msa0699010-mRNA-1:exon:20893;Parent=Msa0699010-mRNA-1
chr4_4 exon 69547913 69548080 69547913 ID=Msa0699010-mRNA-1:exon:20894;Parent=Msa0699010-mRNA-1
chr4_4 exon 69548292 69548336 69548292 ID=Msa0699010-mRNA-1:exon:20895;Parent=Msa0699010-mRNA-1
chr4_4 exon 69548820 69549027 69548820 ID=Msa0699010-mRNA-1:exon:20896;Parent=Msa0699010-mRNA-1
chr4_4 exon 69549158 69549239 69549158 ID=Msa0699010-mRNA-1:exon:20897;Parent=Msa0699010-mRNA-1
chr4_4 exon 69549488 69550477 69549488 ID=Msa0699010-mRNA-1:exon:20898;Parent=Msa0699010-mRNA-1
chr4_4 five_prime_UTR 69547134 69547306 69547134 ID=Msa0699010-mRNA-1:five_prime_utr;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69547307 69547711 69547307 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69547913 69548080 69547913 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69548292 69548336 69548292 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69548820 69549027 69548820 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69549158 69549239 69549158 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 CDS 69549488 69549995 69549488 ID=Msa0699010-mRNA-1:cds;Parent=Msa0699010-mRNA-1
chr4_4 three_prime_UTR 69549996 69550477 69549996 ID=Msa0699010-mRNA-1:three_prime_utr;Parent=Msa0699010-mRNA-1
Gene Sequence

>Msa0699010

ATGGATCTCATTCATGGACTATTTATTCTTTGGATTACCTTAATACTACTTAATTTGAGAAATGTGGATGGAAGGAACCACTTTCACACCAAGCAAAAGAAAGTATCTTCCCTTGTTCCTTCTAAGTCTTCAAATGATCCAGAAAATCCTTCCAACTCTTCTCCTAGTGTTCCTTCTGACCCTTATCCAAATGATCCTCGGGATTCGTCTTCGGACTGTATTTTCGATGTTACATCATTTGGAGCAATTGGAGATGGTTCAGCTGATGACACACCTGCATTCAAGAAAGCATGGAAAGCAGCTTGTGCTGTAGAGTCAGGAGTTCTTCTGGTTCCAGAAAATTACACTTTTATGATCACTTCAATTATTTTTTCAGGTCCATGCAAACCAGGAATAGTATTTCAAGTGGATGGTATGCTAATGGCACCAGATGGACCTGATTCATGGCCAGAGGCAGATAGCAGAAATCAATGGCTTGTGTTCTATAAACTTGACCAAATGAGTTTTAATGGTACAGGAACCATTGAAGGAAATGGAGATCAATGGTGGGATCTTCCCTGCAAACCTCACAGGGGTCCAGATGGAAAAACTTTGTCAGGACCATGTGGAAGTCCAGCTATGATACGGTTCTTCATGAGCTCCAATTTGTATGTGAATGGACTAAAAATTCAAAACAGTCCTCAGTTCCACATGATATTTAATGGCTGCCAAGGAGTAATGATCAATATGTTATCCATTTCTGCACCAAAACTTAGCCCCAACACTGATGGAATCCATGTTGAAAACTCACAAGATGTTGGGATATACAACTCCTTAATAAGCAATGGTGATGATTGCATTTCAATTGGACCCGGGACTTCAAATGTGGAAATAGCTGGTGTAACTTGTGGTCCTAGCCACGGGATTAGCATAGGAAGTCTTGGTGTGCACAATTCCCAAGCATGTGTCTCAAACTTAACCGTCCGTGACACAGTCATAAGAGAATCAGACAATGGACTAAGAATAAAGACATGGCAAGGTGGAATGGGATCTGTGTCAAATTTAAAATTTGAGAACATACAAATGGAGAATGTAGGAAACTGCATCCTTATAGACCAATACTATTGTTTGACAAAGGAATGTCTAAATCAAACATCAGCAGTACATGTTAATGATGTGTCCTACAAAAACATTAAGGGTACATATGATGTTAGAACTGCTCCTATTCATTTTGCTTGTAGTGACACTGTTGCTTGTACAAACATAACACTCTCTGAGGTTGAGCTTTTGCCATTTGAAGGTGAATTGTTGGATGACCCTTTTTGTTGGAATGCTTATGGAGATCAAGAGACTTTGACTATACCTCCAATTAATTGTTTAAGGGAAGGTGACCCTGAGACTGTGGGTGATCTTTCTACATATCAATGTAGTAATTAG
Protein sequence

>Msa0699010

MDLIHGLFILWITLILLNLRNVDGRNHFHTKQKKVSSLVPSKSSNDPENPSNSSPSVPSDPYPNDPRDSSSDCIFDVTSFGAIGDGSADDTPAFKKAWKAACAVESGVLLVPENYTFMITSIIFSGPCKPGIVFQVDGMLMAPDGPDSWPEADSRNQWLVFYKLDQMSFNGTGTIEGNGDQWWDLPCKPHRGPDGKTLSGPCGSPAMIRFFMSSNLYVNGLKIQNSPQFHMIFNGCQGVMINMLSISAPKLSPNTDGIHVENSQDVGIYNSLISNGDDCISIGPGTSNVEIAGVTCGPSHGISIGSLGVHNSQACVSNLTVRDTVIRESDNGLRIKTWQGGMGSVSNLKFENIQMENVGNCILIDQYYCLTKECLNQTSAVHVNDVSYKNIKGTYDVRTAPIHFACSDTVACTNITLSEVELLPFEGELLDDPFCWNAYGDQETLTIPPINCLREGDPETVGDLSTYQCSN*