Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566980 | KEH20775.1 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 9.00e-69 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566980 | A0A072U4M2 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 4.30e-69 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0566980 | Msa0610280 | 0.928499 | 2.648477e-92 | -8.615850e-47 |
| Msa0566980 | Msa0658650 | 0.823505 | 1.335915e-53 | -8.615850e-47 |
| Msa0566980 | Msa0703460 | 0.834702 | 2.572914e-56 | -8.615850e-47 |
| Msa0566980 | Msa1088440 | 0.999761 | 0.000000e+00 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0566980 | MtrunA17_Chr8g0381641 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 8.28e-73 | 211 |
| Msa0566980 | MtrunA17_Chr8g0381621 | 75.238 | 105 | 21 | 4 | 1 | 103 | 1 | 102 | 1.70e-49 | 152 |
| Msa0566980 | MtrunA17_Chr8g0381631 | 57.831 | 83 | 22 | 2 | 25 | 107 | 44 | 113 | 1.44e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTTGTGGTTTCGGTTT+TGG | 0.230297 | 4_1:+68204535 | None:intergenic |
| ACCCGTAATTGTTGTGGTTT+CGG | 0.263916 | 4_1:+68204529 | None:intergenic |
| AACCGAAACCACAACAATTA+CGG | 0.350207 | 4_1:-68204531 | Msa0566980:CDS |
| GATTGATCTTCCTCAACTAT+TGG | 0.360870 | 4_1:+68204423 | None:intergenic |
| CCTCATTGATGACATGAGTT+TGG | 0.382010 | 4_1:+68204251 | None:intergenic |
| ACCGAAACCACAACAATTAC+GGG | 0.390353 | 4_1:-68204530 | Msa0566980:CDS |
| AGATCAATCCTACTATTTAG+AGG | 0.394992 | 4_1:-68204409 | Msa0566980:CDS |
| TTACGAACCCGCATACTATC+CGG | 0.409986 | 4_1:-68204487 | Msa0566980:CDS |
| TACGAACCCGCATACTATCC+GGG | 0.436849 | 4_1:-68204486 | Msa0566980:CDS |
| ACGACGAACCTCTAAATAGT+AGG | 0.482372 | 4_1:+68204401 | None:intergenic |
| AGACCGATCGACGAGGTTTG+AGG | 0.483942 | 4_1:-68204365 | Msa0566980:CDS |
| GATCGACGAGGTTTGAGGTA+TGG | 0.517220 | 4_1:-68204360 | Msa0566980:CDS |
| GCGTCGTGTTGCAATATATG+AGG | 0.518107 | 4_1:-68204448 | Msa0566980:CDS |
| ATACCTCAAACCTCGTCGAT+CGG | 0.542925 | 4_1:+68204362 | None:intergenic |
| TTATTCAACACGAGCATAGG+AGG | 0.567055 | 4_1:-68204284 | Msa0566980:CDS |
| CAAACTCATGTCATCAATGA+GGG | 0.571315 | 4_1:-68204250 | Msa0566980:CDS |
| GACTCCACCCGTAATTGTTG+TGG | 0.580902 | 4_1:+68204523 | None:intergenic |
| GTGATGCCCCGGATAGTATG+CGG | 0.591042 | 4_1:+68204479 | None:intergenic |
| CCAAACTCATGTCATCAATG+AGG | 0.606593 | 4_1:-68204251 | Msa0566980:CDS |
| TGATGCCCCGGATAGTATGC+GGG | 0.606955 | 4_1:+68204480 | None:intergenic |
| ATATGAGGAACCAATAGTTG+AGG | 0.635820 | 4_1:-68204433 | Msa0566980:CDS |
| TCAACACGAGCATAGGAGGA+CGG | 0.662040 | 4_1:-68204280 | Msa0566980:CDS |
| GAAACCACAACAATTACGGG+TGG | 0.673197 | 4_1:-68204527 | Msa0566980:CDS |
| ACGAACCCGCATACTATCCG+GGG | 0.673282 | 4_1:-68204485 | Msa0566980:CDS |
| GAGACTGAGACCGATCGACG+AGG | 0.700755 | 4_1:-68204372 | Msa0566980:CDS |
| AAGTGTTGATGAAGAAGCTG+AGG | 0.703686 | 4_1:-68204310 | Msa0566980:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATCAATCCTACTATTTAG+AGG | - | chr4_1:68204376-68204395 | Msa0566980:CDS | 30.0% | |
| ! | AATTGTTGTGGTTTCGGTTT+TGG | + | chr4_1:68204253-68204272 | None:intergenic | 35.0% |
| AACCGAAACCACAACAATTA+CGG | - | chr4_1:68204254-68204273 | Msa0566980:CDS | 35.0% | |
| ATATGAGGAACCAATAGTTG+AGG | - | chr4_1:68204352-68204371 | Msa0566980:CDS | 35.0% | |
| GATTGATCTTCCTCAACTAT+TGG | + | chr4_1:68204365-68204384 | None:intergenic | 35.0% | |
| ! | CTTTTATTCAACACGAGCAT+AGG | - | chr4_1:68204498-68204517 | Msa0566980:CDS | 35.0% |
| CAAACTCATGTCATCAATGA+GGG | - | chr4_1:68204535-68204554 | Msa0566980:CDS | 35.0% | |
| ACCGAAACCACAACAATTAC+GGG | - | chr4_1:68204255-68204274 | Msa0566980:CDS | 40.0% | |
| ACCCGTAATTGTTGTGGTTT+CGG | + | chr4_1:68204259-68204278 | None:intergenic | 40.0% | |
| ACGACGAACCTCTAAATAGT+AGG | + | chr4_1:68204387-68204406 | None:intergenic | 40.0% | |
| !! | AAGTGTTGATGAAGAAGCTG+AGG | - | chr4_1:68204475-68204494 | Msa0566980:CDS | 40.0% |
| TTATTCAACACGAGCATAGG+AGG | - | chr4_1:68204501-68204520 | Msa0566980:CDS | 40.0% | |
| ! | CCTCATTGATGACATGAGTT+TGG | + | chr4_1:68204537-68204556 | None:intergenic | 40.0% |
| CCAAACTCATGTCATCAATG+AGG | - | chr4_1:68204534-68204553 | Msa0566980:CDS | 40.0% | |
| GAAACCACAACAATTACGGG+TGG | - | chr4_1:68204258-68204277 | Msa0566980:CDS | 45.0% | |
| TTACGAACCCGCATACTATC+CGG | - | chr4_1:68204298-68204317 | Msa0566980:CDS | 45.0% | |
| ! | TTGCAACACGACGCTTTTGA+TGG | + | chr4_1:68204331-68204350 | None:intergenic | 45.0% |
| GCGTCGTGTTGCAATATATG+AGG | - | chr4_1:68204337-68204356 | Msa0566980:CDS | 45.0% | |
| ATACCTCAAACCTCGTCGAT+CGG | + | chr4_1:68204426-68204445 | None:intergenic | 45.0% | |
| GACTCCACCCGTAATTGTTG+TGG | + | chr4_1:68204265-68204284 | None:intergenic | 50.0% | |
| TACGAACCCGCATACTATCC+GGG | - | chr4_1:68204299-68204318 | Msa0566980:CDS | 50.0% | |
| ! | GATCGACGAGGTTTGAGGTA+TGG | - | chr4_1:68204425-68204444 | Msa0566980:CDS | 50.0% |
| TCAACACGAGCATAGGAGGA+CGG | - | chr4_1:68204505-68204524 | Msa0566980:CDS | 50.0% | |
| ACGAACCCGCATACTATCCG+GGG | - | chr4_1:68204300-68204319 | Msa0566980:CDS | 55.0% | |
| TGATGCCCCGGATAGTATGC+GGG | + | chr4_1:68204308-68204327 | None:intergenic | 55.0% | |
| GTGATGCCCCGGATAGTATG+CGG | + | chr4_1:68204309-68204328 | None:intergenic | 55.0% | |
| !!! | CGCTTTTGATGGTGATGCCC+CGG | + | chr4_1:68204320-68204339 | None:intergenic | 55.0% |
| AGACCGATCGACGAGGTTTG+AGG | - | chr4_1:68204420-68204439 | Msa0566980:CDS | 55.0% | |
| GAGACTGAGACCGATCGACG+AGG | - | chr4_1:68204413-68204432 | Msa0566980:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 68204242 | 68204565 | 68204242 | ID=Msa0566980;Name=Msa0566980 |
| chr4_1 | mRNA | 68204242 | 68204565 | 68204242 | ID=Msa0566980-mRNA-1;Parent=Msa0566980;Name=Msa0566980-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr4_1 | exon | 68204242 | 68204565 | 68204242 | ID=Msa0566980-mRNA-1:exon:22511;Parent=Msa0566980-mRNA-1 |
| chr4_1 | CDS | 68204242 | 68204565 | 68204242 | ID=Msa0566980-mRNA-1:cds;Parent=Msa0566980-mRNA-1 |
| Gene Sequence |
| Protein sequence |
