Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0610280 | KEH20775.1 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 9.00e-69 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0610280 | A0A072U4M2 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 4.30e-69 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0100250 | Msa0610280 | 0.801479 | 8.830738e-49 | -8.615850e-47 |
| Msa0610280 | Msa0616610 | 0.822954 | 1.796286e-53 | -8.615850e-47 |
| Msa0610280 | Msa0616710 | 0.805003 | 1.646109e-49 | -8.615850e-47 |
| Msa0610280 | Msa0634420 | 0.809369 | 1.957958e-50 | -8.615850e-47 |
| Msa0610280 | Msa0658650 | 0.901237 | 3.284039e-78 | -8.615850e-47 |
| Msa0610280 | Msa0703460 | 0.935984 | 3.579255e-97 | -8.615850e-47 |
| Msa0610280 | Msa0738860 | 0.807147 | 5.824726e-50 | -8.615850e-47 |
| Msa0610280 | Msa0738870 | 0.801458 | 8.921381e-49 | -8.615850e-47 |
| Msa0610280 | Msa0758820 | 0.814964 | 1.177905e-51 | -8.615850e-47 |
| Msa0610280 | Msa0838660 | 0.814313 | 1.641532e-51 | -8.615850e-47 |
| Msa0610280 | Msa0850740 | 0.806875 | 6.649797e-50 | -8.615850e-47 |
| Msa0610280 | Msa0859520 | 0.803642 | 3.163123e-49 | -8.615850e-47 |
| Msa0610280 | Msa0859530 | 0.815978 | 7.007574e-52 | -8.615850e-47 |
| Msa0610280 | Msa0971860 | 0.803646 | 3.156976e-49 | -8.615850e-47 |
| Msa0610280 | Msa1088440 | 0.929629 | 5.283487e-93 | -8.615850e-47 |
| Msa0610280 | Msa1198450 | 0.840512 | 8.313905e-58 | -8.615850e-47 |
| Msa0610280 | Msa1220740 | 0.805796 | 1.123059e-49 | -8.615850e-47 |
| Msa0610280 | Msa1302780 | 0.823175 | 1.595768e-53 | -8.615850e-47 |
| Msa0132620 | Msa0610280 | 0.807235 | 5.580205e-50 | -8.615850e-47 |
| Msa0155610 | Msa0610280 | 0.801039 | 1.086637e-48 | -8.615850e-47 |
| Msa0200840 | Msa0610280 | 0.817967 | 2.505040e-52 | -8.615850e-47 |
| Msa0200870 | Msa0610280 | 0.803761 | 2.988023e-49 | -8.615850e-47 |
| Msa0203180 | Msa0610280 | 0.815776 | 7.772267e-52 | -8.615850e-47 |
| Msa0243470 | Msa0610280 | 0.809948 | 1.470092e-50 | -8.615850e-47 |
| Msa0246300 | Msa0610280 | 0.823673 | 1.220236e-53 | -8.615850e-47 |
| Msa0281940 | Msa0610280 | 0.827568 | 1.456207e-54 | -8.615850e-47 |
| Msa0284870 | Msa0610280 | 0.816091 | 6.610862e-52 | -8.615850e-47 |
| Msa0503090 | Msa0610280 | 0.804045 | 2.608175e-49 | -8.615850e-47 |
| Msa0534420 | Msa0610280 | 0.813024 | 3.155087e-51 | -8.615850e-47 |
| Msa0538260 | Msa0610280 | 0.818185 | 2.236605e-52 | -8.615850e-47 |
| Msa0538270 | Msa0610280 | 0.802110 | 6.555167e-49 | -8.615850e-47 |
| Msa0557490 | Msa0610280 | 0.803775 | 2.968343e-49 | -8.615850e-47 |
| Msa0566980 | Msa0610280 | 0.928499 | 2.648477e-92 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0610280 | MtrunA17_Chr8g0381641 | 93.458 | 107 | 7 | 0 | 1 | 107 | 1 | 107 | 8.28e-73 | 211 |
| Msa0610280 | MtrunA17_Chr8g0381621 | 75.238 | 105 | 21 | 4 | 1 | 103 | 1 | 102 | 1.70e-49 | 152 |
| Msa0610280 | MtrunA17_Chr8g0381631 | 57.831 | 83 | 22 | 2 | 25 | 107 | 44 | 113 | 1.44e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTTGTGGTTTCGGTTT+TGG | 0.230297 | 4_2:+57988049 | None:intergenic |
| ACCCGTAATTGTTGTGGTTT+CGG | 0.263916 | 4_2:+57988043 | None:intergenic |
| AACCGAAACCACAACAATTA+CGG | 0.350207 | 4_2:-57988045 | Msa0610280:CDS |
| GATTGATCTTCCTCAACTAT+TGG | 0.360870 | 4_2:+57987937 | None:intergenic |
| CCTCATTGATGACATGAGTT+TGG | 0.382010 | 4_2:+57987765 | None:intergenic |
| ACCGAAACCACAACAATTAC+GGG | 0.390353 | 4_2:-57988044 | Msa0610280:CDS |
| AGATCAATCCTACTATTTAG+AGG | 0.394992 | 4_2:-57987923 | Msa0610280:CDS |
| TTACGAACCCGCATACTATC+CGG | 0.409986 | 4_2:-57988001 | Msa0610280:CDS |
| TACGAACCCGCATACTATCC+GGG | 0.436849 | 4_2:-57988000 | Msa0610280:CDS |
| ACGACGAACCTCTAAATAGT+AGG | 0.482372 | 4_2:+57987915 | None:intergenic |
| AGACCGATCGACGAGGTTTG+AGG | 0.483942 | 4_2:-57987879 | Msa0610280:CDS |
| GATCGACGAGGTTTGAGGTA+TGG | 0.517220 | 4_2:-57987874 | Msa0610280:CDS |
| GCGTCGTGTTGCAATATATG+AGG | 0.518107 | 4_2:-57987962 | Msa0610280:CDS |
| ATACCTCAAACCTCGTCGAT+CGG | 0.542925 | 4_2:+57987876 | None:intergenic |
| TTATTCAACACGAGCATAGG+AGG | 0.567055 | 4_2:-57987798 | Msa0610280:CDS |
| CAAACTCATGTCATCAATGA+GGG | 0.571315 | 4_2:-57987764 | Msa0610280:CDS |
| GACTCCACCCGTAATTGTTG+TGG | 0.580902 | 4_2:+57988037 | None:intergenic |
| GTGATGCCCCGGATAGTATG+CGG | 0.591042 | 4_2:+57987993 | None:intergenic |
| CCAAACTCATGTCATCAATG+AGG | 0.606593 | 4_2:-57987765 | Msa0610280:CDS |
| TGATGCCCCGGATAGTATGC+GGG | 0.606955 | 4_2:+57987994 | None:intergenic |
| ATATGAGGAACCAATAGTTG+AGG | 0.635820 | 4_2:-57987947 | Msa0610280:CDS |
| TCAACACGAGCATAGGAGGA+CGG | 0.662040 | 4_2:-57987794 | Msa0610280:CDS |
| GAAACCACAACAATTACGGG+TGG | 0.673197 | 4_2:-57988041 | Msa0610280:CDS |
| ACGAACCCGCATACTATCCG+GGG | 0.673282 | 4_2:-57987999 | Msa0610280:CDS |
| GAGACTGAGACCGATCGACG+AGG | 0.700755 | 4_2:-57987886 | Msa0610280:CDS |
| AAGTGTTGATGAAGAAGCTG+AGG | 0.703686 | 4_2:-57987824 | Msa0610280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATCAATCCTACTATTTAG+AGG | - | chr4_2:57987890-57987909 | Msa0610280:CDS | 30.0% | |
| ! | AATTGTTGTGGTTTCGGTTT+TGG | + | chr4_2:57987767-57987786 | None:intergenic | 35.0% |
| AACCGAAACCACAACAATTA+CGG | - | chr4_2:57987768-57987787 | Msa0610280:CDS | 35.0% | |
| ATATGAGGAACCAATAGTTG+AGG | - | chr4_2:57987866-57987885 | Msa0610280:CDS | 35.0% | |
| GATTGATCTTCCTCAACTAT+TGG | + | chr4_2:57987879-57987898 | None:intergenic | 35.0% | |
| ! | CTTTTATTCAACACGAGCAT+AGG | - | chr4_2:57988012-57988031 | Msa0610280:CDS | 35.0% |
| CAAACTCATGTCATCAATGA+GGG | - | chr4_2:57988049-57988068 | Msa0610280:CDS | 35.0% | |
| ACCGAAACCACAACAATTAC+GGG | - | chr4_2:57987769-57987788 | Msa0610280:CDS | 40.0% | |
| ACCCGTAATTGTTGTGGTTT+CGG | + | chr4_2:57987773-57987792 | None:intergenic | 40.0% | |
| ACGACGAACCTCTAAATAGT+AGG | + | chr4_2:57987901-57987920 | None:intergenic | 40.0% | |
| !! | AAGTGTTGATGAAGAAGCTG+AGG | - | chr4_2:57987989-57988008 | Msa0610280:CDS | 40.0% |
| TTATTCAACACGAGCATAGG+AGG | - | chr4_2:57988015-57988034 | Msa0610280:CDS | 40.0% | |
| ! | CCTCATTGATGACATGAGTT+TGG | + | chr4_2:57988051-57988070 | None:intergenic | 40.0% |
| CCAAACTCATGTCATCAATG+AGG | - | chr4_2:57988048-57988067 | Msa0610280:CDS | 40.0% | |
| GAAACCACAACAATTACGGG+TGG | - | chr4_2:57987772-57987791 | Msa0610280:CDS | 45.0% | |
| TTACGAACCCGCATACTATC+CGG | - | chr4_2:57987812-57987831 | Msa0610280:CDS | 45.0% | |
| ! | TTGCAACACGACGCTTTTGA+TGG | + | chr4_2:57987845-57987864 | None:intergenic | 45.0% |
| GCGTCGTGTTGCAATATATG+AGG | - | chr4_2:57987851-57987870 | Msa0610280:CDS | 45.0% | |
| ATACCTCAAACCTCGTCGAT+CGG | + | chr4_2:57987940-57987959 | None:intergenic | 45.0% | |
| GACTCCACCCGTAATTGTTG+TGG | + | chr4_2:57987779-57987798 | None:intergenic | 50.0% | |
| TACGAACCCGCATACTATCC+GGG | - | chr4_2:57987813-57987832 | Msa0610280:CDS | 50.0% | |
| ! | GATCGACGAGGTTTGAGGTA+TGG | - | chr4_2:57987939-57987958 | Msa0610280:CDS | 50.0% |
| TCAACACGAGCATAGGAGGA+CGG | - | chr4_2:57988019-57988038 | Msa0610280:CDS | 50.0% | |
| ACGAACCCGCATACTATCCG+GGG | - | chr4_2:57987814-57987833 | Msa0610280:CDS | 55.0% | |
| TGATGCCCCGGATAGTATGC+GGG | + | chr4_2:57987822-57987841 | None:intergenic | 55.0% | |
| GTGATGCCCCGGATAGTATG+CGG | + | chr4_2:57987823-57987842 | None:intergenic | 55.0% | |
| !!! | CGCTTTTGATGGTGATGCCC+CGG | + | chr4_2:57987834-57987853 | None:intergenic | 55.0% |
| AGACCGATCGACGAGGTTTG+AGG | - | chr4_2:57987934-57987953 | Msa0610280:CDS | 55.0% | |
| GAGACTGAGACCGATCGACG+AGG | - | chr4_2:57987927-57987946 | Msa0610280:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 57987756 | 57988079 | 57987756 | ID=Msa0610280;Name=Msa0610280 |
| chr4_2 | mRNA | 57987756 | 57988079 | 57987756 | ID=Msa0610280-mRNA-1;Parent=Msa0610280;Name=Msa0610280-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr4_2 | exon | 57987756 | 57988079 | 57987756 | ID=Msa0610280-mRNA-1:exon:15155;Parent=Msa0610280-mRNA-1 |
| chr4_2 | CDS | 57987756 | 57988079 | 57987756 | ID=Msa0610280-mRNA-1:cds;Parent=Msa0610280-mRNA-1 |
| Gene Sequence |
| Protein sequence |
