AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0658650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0658650 MtrunA17_Chr8g0381641 92.523 107 8 0 1 107 1 107 4.34e-72 209
Msa0658650 MtrunA17_Chr8g0381621 74.286 105 22 4 1 103 1 102 6.90e-49 150
Msa0658650 MtrunA17_Chr8g0381631 57.831 83 22 2 25 107 44 113 1.31e-21 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGTTGTGGTTTCGGTTT+TGG 0.230297 4_3:-68852084 None:intergenic
ACCCGTAATTGTTGTGGTTT+CGG 0.263916 4_3:-68852090 None:intergenic
AACCGAAACCACAACAATTA+CGG 0.350207 4_3:+68852088 Msa0658650:CDS
GATTGATCTTCCTCAACTAT+TGG 0.360870 4_3:-68852196 None:intergenic
CCTCATTGATGACATGAGTT+TGG 0.382010 4_3:-68852368 None:intergenic
ACCGAAACCACAACAATTAC+GGG 0.390353 4_3:+68852089 Msa0658650:CDS
AGATCAATCCTACTATTTAG+AGG 0.394992 4_3:+68852210 Msa0658650:CDS
TTACGAACCCACATACTATC+CGG 0.475257 4_3:+68852132 Msa0658650:CDS
ACGACGAACCTCTAAATAGT+AGG 0.482372 4_3:-68852218 None:intergenic
AGACCGATCGACGAGGTTTG+AGG 0.483942 4_3:+68852254 Msa0658650:CDS
TACGAACCCACATACTATCC+GGG 0.510321 4_3:+68852133 Msa0658650:CDS
GATCGACGAGGTTTGAGGTA+TGG 0.517220 4_3:+68852259 Msa0658650:CDS
GCGTCGTGTTGCAATATATG+AGG 0.518107 4_3:+68852171 Msa0658650:CDS
ATACCTCAAACCTCGTCGAT+CGG 0.542925 4_3:-68852257 None:intergenic
GTGATGCCCCGGATAGTATG+TGG 0.565451 4_3:-68852140 None:intergenic
TTATTCAACACGAGCATAGG+AGG 0.567055 4_3:+68852335 Msa0658650:CDS
CAAACTCATGTCATCAATGA+GGG 0.571315 4_3:+68852369 Msa0658650:CDS
GACTCCACCCGTAATTGTTG+TGG 0.580902 4_3:-68852096 None:intergenic
CCAAACTCATGTCATCAATG+AGG 0.606593 4_3:+68852368 Msa0658650:CDS
ATATGAGGAACCAATAGTTG+AGG 0.635820 4_3:+68852186 Msa0658650:CDS
TCAACACGAGCATAGGAGGA+CGG 0.662040 4_3:+68852339 Msa0658650:CDS
GAAACCACAACAATTACGGG+TGG 0.673197 4_3:+68852092 Msa0658650:CDS
ACGAACCCACATACTATCCG+GGG 0.676259 4_3:+68852134 Msa0658650:CDS
TGATGCCCCGGATAGTATGT+GGG 0.689506 4_3:-68852139 None:intergenic
GAGACTGAGACCGATCGACG+AGG 0.700755 4_3:+68852247 Msa0658650:CDS
AAGTGTTGATGAAGAAGCTG+AGG 0.703686 4_3:+68852309 Msa0658650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGATCAATCCTACTATTTAG+AGG + chr4_3:68852210-68852229 Msa0658650:CDS 30.0%
! AATTGTTGTGGTTTCGGTTT+TGG - chr4_3:68852087-68852106 None:intergenic 35.0%
AACCGAAACCACAACAATTA+CGG + chr4_3:68852088-68852107 Msa0658650:CDS 35.0%
ATATGAGGAACCAATAGTTG+AGG + chr4_3:68852186-68852205 Msa0658650:CDS 35.0%
GATTGATCTTCCTCAACTAT+TGG - chr4_3:68852199-68852218 None:intergenic 35.0%
! CTTTTATTCAACACGAGCAT+AGG + chr4_3:68852332-68852351 Msa0658650:CDS 35.0%
CAAACTCATGTCATCAATGA+GGG + chr4_3:68852369-68852388 Msa0658650:CDS 35.0%
ACCGAAACCACAACAATTAC+GGG + chr4_3:68852089-68852108 Msa0658650:CDS 40.0%
ACCCGTAATTGTTGTGGTTT+CGG - chr4_3:68852093-68852112 None:intergenic 40.0%
TTACGAACCCACATACTATC+CGG + chr4_3:68852132-68852151 Msa0658650:CDS 40.0%
ACGACGAACCTCTAAATAGT+AGG - chr4_3:68852221-68852240 None:intergenic 40.0%
!! AAGTGTTGATGAAGAAGCTG+AGG + chr4_3:68852309-68852328 Msa0658650:CDS 40.0%
TTATTCAACACGAGCATAGG+AGG + chr4_3:68852335-68852354 Msa0658650:CDS 40.0%
! CCTCATTGATGACATGAGTT+TGG - chr4_3:68852371-68852390 None:intergenic 40.0%
CCAAACTCATGTCATCAATG+AGG + chr4_3:68852368-68852387 Msa0658650:CDS 40.0%
GAAACCACAACAATTACGGG+TGG + chr4_3:68852092-68852111 Msa0658650:CDS 45.0%
TACGAACCCACATACTATCC+GGG + chr4_3:68852133-68852152 Msa0658650:CDS 45.0%
! TTGCAACACGACGCTTTTGA+TGG - chr4_3:68852165-68852184 None:intergenic 45.0%
GCGTCGTGTTGCAATATATG+AGG + chr4_3:68852171-68852190 Msa0658650:CDS 45.0%
ATACCTCAAACCTCGTCGAT+CGG - chr4_3:68852260-68852279 None:intergenic 45.0%
GACTCCACCCGTAATTGTTG+TGG - chr4_3:68852099-68852118 None:intergenic 50.0%
ACGAACCCACATACTATCCG+GGG + chr4_3:68852134-68852153 Msa0658650:CDS 50.0%
TGATGCCCCGGATAGTATGT+GGG - chr4_3:68852142-68852161 None:intergenic 50.0%
! GATCGACGAGGTTTGAGGTA+TGG + chr4_3:68852259-68852278 Msa0658650:CDS 50.0%
TCAACACGAGCATAGGAGGA+CGG + chr4_3:68852339-68852358 Msa0658650:CDS 50.0%
GTGATGCCCCGGATAGTATG+TGG - chr4_3:68852143-68852162 None:intergenic 55.0%
!!! CGCTTTTGATGGTGATGCCC+CGG - chr4_3:68852154-68852173 None:intergenic 55.0%
AGACCGATCGACGAGGTTTG+AGG + chr4_3:68852254-68852273 Msa0658650:CDS 55.0%
GAGACTGAGACCGATCGACG+AGG + chr4_3:68852247-68852266 Msa0658650:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 68852076 68852399 68852076 ID=Msa0658650;Name=Msa0658650
chr4_3 mRNA 68852076 68852399 68852076 ID=Msa0658650-mRNA-1;Parent=Msa0658650;Name=Msa0658650-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|107
chr4_3 exon 68852076 68852399 68852076 ID=Msa0658650-mRNA-1:exon:20024;Parent=Msa0658650-mRNA-1
chr4_3 CDS 68852076 68852399 68852076 ID=Msa0658650-mRNA-1:cds;Parent=Msa0658650-mRNA-1
Gene Sequence

>Msa0658650

ATGGCATACCAAAACCGAAACCACAACAATTACGGGTGGAGTCACATGCACGAAATTTACGAACCCACATACTATCCGGGGCATCACCATCAAAAGCGTCGTGTTGCAATATATGAGGAACCAATAGTTGAGGAAGATCAATCCTACTATTTAGAGGTTCGTCGTGAGACTGAGACTGAGACCGATCGACGAGGTTTGAGGTATGGAGATCAAAATGTTAGCACTACTTATGAAAGTGTTGATGAAGAAGCTGAGGCTTTTATTCAACACGAGCATAGGAGGACGGCATTAGCCAAACTCATGTCATCAATGAGGGCTACTTAA

Protein sequence

>Msa0658650

MAYQNRNHNNYGWSHMHEIYEPTYYPGHHHQKRRVAIYEEPIVEEDQSYYLEVRRETETETDRRGLRYGDQNVSTTYESVDEEAEAFIQHEHRRTALAKLMSSMRAT*