Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703460 | KEH20775.1 | 92.523 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 7.17e-68 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703460 | A0A072U4M2 | 92.523 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 3.42e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008850 | Msa0703460 | 0.807195 | 5.691348e-50 | -8.615850e-47 |
| Msa0038310 | Msa0703460 | 0.800685 | 1.283490e-48 | -8.615850e-47 |
| Msa0047470 | Msa0703460 | 0.807918 | 3.997445e-50 | -8.615850e-47 |
| Msa0060600 | Msa0703460 | 0.808960 | 2.395812e-50 | -8.615850e-47 |
| Msa0610280 | Msa0703460 | 0.935984 | 3.579255e-97 | -8.615850e-47 |
| Msa0616610 | Msa0703460 | 0.820434 | 6.872399e-53 | -8.615850e-47 |
| Msa0617830 | Msa0703460 | 0.824825 | 6.542325e-54 | -8.615850e-47 |
| Msa0620750 | Msa0703460 | 0.809431 | 1.899144e-50 | -8.615850e-47 |
| Msa0634420 | Msa0703460 | 0.809282 | 2.044177e-50 | -8.615850e-47 |
| Msa0656680 | Msa0703460 | 0.808423 | 3.119674e-50 | -8.615850e-47 |
| Msa0658650 | Msa0703460 | 0.872621 | 2.763505e-67 | -8.615850e-47 |
| Msa0703460 | Msa0714340 | 0.832334 | 1.003204e-55 | -8.615850e-47 |
| Msa0703460 | Msa0714380 | 0.816424 | 5.569523e-52 | -8.615850e-47 |
| Msa0703460 | Msa0738850 | 0.824564 | 7.538518e-54 | -8.615850e-47 |
| Msa0703460 | Msa0738860 | 0.825398 | 4.791212e-54 | -8.615850e-47 |
| Msa0703460 | Msa0738870 | 0.803311 | 3.703005e-49 | -8.615850e-47 |
| Msa0703460 | Msa0763880 | 0.810508 | 1.112962e-50 | -8.615850e-47 |
| Msa0703460 | Msa0763910 | 0.804216 | 2.402657e-49 | -8.615850e-47 |
| Msa0703460 | Msa0783260 | 0.806016 | 1.009633e-49 | -8.615850e-47 |
| Msa0703460 | Msa0783270 | 0.822420 | 2.391090e-53 | -8.615850e-47 |
| Msa0703460 | Msa0787720 | 0.808914 | 2.449982e-50 | -8.615850e-47 |
| Msa0703460 | Msa0812660 | 0.803301 | 3.722185e-49 | -8.615850e-47 |
| Msa0703460 | Msa0838660 | 0.811318 | 7.434778e-51 | -8.615850e-47 |
| Msa0703460 | Msa0859520 | 0.802470 | 5.524477e-49 | -8.615850e-47 |
| Msa0703460 | Msa0859530 | 0.840256 | 9.700059e-58 | -8.615850e-47 |
| Msa0703460 | Msa0971860 | 0.820739 | 5.849460e-53 | -8.615850e-47 |
| Msa0703460 | Msa1088440 | 0.836010 | 1.201795e-56 | -8.615850e-47 |
| Msa0703460 | Msa1116140 | 0.801866 | 7.356018e-49 | -8.615850e-47 |
| Msa0703460 | Msa1198450 | 0.846340 | 2.309441e-59 | -8.615850e-47 |
| Msa0703460 | Msa1241820 | 0.811162 | 8.035647e-51 | -8.615850e-47 |
| Msa0703460 | Msa1302780 | 0.814967 | 1.176334e-51 | -8.615850e-47 |
| Msa0703460 | Msa1378470 | 0.811983 | 5.329137e-51 | -8.615850e-47 |
| Msa0703460 | Msa1431730 | 0.818082 | 2.359380e-52 | -8.615850e-47 |
| Msa0132620 | Msa0703460 | 0.812763 | 3.599149e-51 | -8.615850e-47 |
| Msa0155610 | Msa0703460 | 0.805674 | 1.190954e-49 | -8.615850e-47 |
| Msa0200840 | Msa0703460 | 0.807411 | 5.122760e-50 | -8.615850e-47 |
| Msa0203180 | Msa0703460 | 0.803037 | 4.219376e-49 | -8.615850e-47 |
| Msa0243470 | Msa0703460 | 0.806805 | 6.882046e-50 | -8.615850e-47 |
| Msa0281940 | Msa0703460 | 0.836685 | 8.096259e-57 | -8.615850e-47 |
| Msa0284870 | Msa0703460 | 0.821560 | 3.784564e-53 | -8.615850e-47 |
| Msa0299790 | Msa0703460 | 0.804423 | 2.175802e-49 | -8.615850e-47 |
| Msa0534420 | Msa0703460 | 0.830794 | 2.402653e-55 | -8.615850e-47 |
| Msa0538260 | Msa0703460 | 0.828302 | 9.693390e-55 | -8.615850e-47 |
| Msa0538270 | Msa0703460 | 0.818762 | 1.654593e-52 | -8.615850e-47 |
| Msa0566980 | Msa0703460 | 0.834702 | 2.572914e-56 | -8.615850e-47 |
| Msa0576600 | Msa0703460 | 0.829265 | 5.670992e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0703460 | MtrunA17_Chr8g0381641 | 92.523 | 107 | 8 | 0 | 1 | 107 | 1 | 107 | 6.59e-72 | 209 |
| Msa0703460 | MtrunA17_Chr8g0381621 | 76.190 | 105 | 20 | 4 | 1 | 103 | 1 | 102 | 2.07e-50 | 154 |
| Msa0703460 | MtrunA17_Chr8g0381631 | 59.036 | 83 | 21 | 2 | 25 | 107 | 44 | 113 | 1.30e-22 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTGTTGTGGTTTCGGTTT+TGG | 0.230297 | 4_4:+75379575 | None:intergenic |
| ACCCGTAATTGTTGTGGTTT+CGG | 0.263916 | 4_4:+75379569 | None:intergenic |
| AACCGAAACCACAACAATTA+CGG | 0.350207 | 4_4:-75379571 | Msa0703460:CDS |
| GATTGATCTTCCTCAACTAT+TGG | 0.360870 | 4_4:+75379463 | None:intergenic |
| CCTCATTGATGACATGAGTT+TGG | 0.382010 | 4_4:+75379291 | None:intergenic |
| ACCGAAACCACAACAATTAC+GGG | 0.390353 | 4_4:-75379570 | Msa0703460:CDS |
| AGATCAATCCTACTATTTAG+AGG | 0.394992 | 4_4:-75379449 | Msa0703460:CDS |
| TTACGAACCCGCATACTATC+CGG | 0.409986 | 4_4:-75379527 | Msa0703460:CDS |
| TACGAACCCGCATACTATCC+GGG | 0.436849 | 4_4:-75379526 | Msa0703460:CDS |
| ACGACGAACCTCTAAATAGT+AGG | 0.482372 | 4_4:+75379441 | None:intergenic |
| AGACCGATCGACGAGGTTTG+AGG | 0.483942 | 4_4:-75379405 | Msa0703460:CDS |
| GATCGACGAGGTTTGAGGTA+TGG | 0.517220 | 4_4:-75379400 | Msa0703460:CDS |
| GCGTCGTGTTGCAATATATG+AGG | 0.518107 | 4_4:-75379488 | Msa0703460:CDS |
| ATACCTCAAACCTCGTCGAT+CGG | 0.542925 | 4_4:+75379402 | None:intergenic |
| TTATTCAACACGAGCATAGG+AGG | 0.555341 | 4_4:-75379324 | Msa0703460:CDS |
| CAAACTCATGTCATCAATGA+GGG | 0.571315 | 4_4:-75379290 | Msa0703460:CDS |
| GACTCCACCCGTAATTGTTG+TGG | 0.580902 | 4_4:+75379563 | None:intergenic |
| GTGATGCCCCGGATAGTATG+CGG | 0.591042 | 4_4:+75379519 | None:intergenic |
| CCAAACTCATGTCATCAATG+AGG | 0.606593 | 4_4:-75379291 | Msa0703460:CDS |
| TGATGCCCCGGATAGTATGC+GGG | 0.606955 | 4_4:+75379520 | None:intergenic |
| TCAACACGAGCATAGGAGGA+TGG | 0.624383 | 4_4:-75379320 | Msa0703460:CDS |
| ATATGAGGAACCAATAGTTG+AGG | 0.635820 | 4_4:-75379473 | Msa0703460:CDS |
| GAAACCACAACAATTACGGG+TGG | 0.673197 | 4_4:-75379567 | Msa0703460:CDS |
| ACGAACCCGCATACTATCCG+GGG | 0.673282 | 4_4:-75379525 | Msa0703460:CDS |
| GAGACTGAGACCGATCGACG+AGG | 0.700755 | 4_4:-75379412 | Msa0703460:CDS |
| AAGTGTTGATGAAGAAGCTG+AGG | 0.703686 | 4_4:-75379350 | Msa0703460:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATCAATCCTACTATTTAG+AGG | - | chr4_4:75379416-75379435 | Msa0703460:CDS | 30.0% | |
| ! | AATTGTTGTGGTTTCGGTTT+TGG | + | chr4_4:75379293-75379312 | None:intergenic | 35.0% |
| AACCGAAACCACAACAATTA+CGG | - | chr4_4:75379294-75379313 | Msa0703460:CDS | 35.0% | |
| ATATGAGGAACCAATAGTTG+AGG | - | chr4_4:75379392-75379411 | Msa0703460:CDS | 35.0% | |
| GATTGATCTTCCTCAACTAT+TGG | + | chr4_4:75379405-75379424 | None:intergenic | 35.0% | |
| ! | CTTTTATTCAACACGAGCAT+AGG | - | chr4_4:75379538-75379557 | Msa0703460:CDS | 35.0% |
| CAAACTCATGTCATCAATGA+GGG | - | chr4_4:75379575-75379594 | Msa0703460:CDS | 35.0% | |
| ACCGAAACCACAACAATTAC+GGG | - | chr4_4:75379295-75379314 | Msa0703460:CDS | 40.0% | |
| ACCCGTAATTGTTGTGGTTT+CGG | + | chr4_4:75379299-75379318 | None:intergenic | 40.0% | |
| ACGACGAACCTCTAAATAGT+AGG | + | chr4_4:75379427-75379446 | None:intergenic | 40.0% | |
| !! | AAGTGTTGATGAAGAAGCTG+AGG | - | chr4_4:75379515-75379534 | Msa0703460:CDS | 40.0% |
| TTATTCAACACGAGCATAGG+AGG | - | chr4_4:75379541-75379560 | Msa0703460:CDS | 40.0% | |
| ! | CCTCATTGATGACATGAGTT+TGG | + | chr4_4:75379577-75379596 | None:intergenic | 40.0% |
| CCAAACTCATGTCATCAATG+AGG | - | chr4_4:75379574-75379593 | Msa0703460:CDS | 40.0% | |
| GAAACCACAACAATTACGGG+TGG | - | chr4_4:75379298-75379317 | Msa0703460:CDS | 45.0% | |
| TTACGAACCCGCATACTATC+CGG | - | chr4_4:75379338-75379357 | Msa0703460:CDS | 45.0% | |
| ! | TTGCAACACGACGCTTTTGA+TGG | + | chr4_4:75379371-75379390 | None:intergenic | 45.0% |
| GCGTCGTGTTGCAATATATG+AGG | - | chr4_4:75379377-75379396 | Msa0703460:CDS | 45.0% | |
| ATACCTCAAACCTCGTCGAT+CGG | + | chr4_4:75379466-75379485 | None:intergenic | 45.0% | |
| GACTCCACCCGTAATTGTTG+TGG | + | chr4_4:75379305-75379324 | None:intergenic | 50.0% | |
| TACGAACCCGCATACTATCC+GGG | - | chr4_4:75379339-75379358 | Msa0703460:CDS | 50.0% | |
| ! | GATCGACGAGGTTTGAGGTA+TGG | - | chr4_4:75379465-75379484 | Msa0703460:CDS | 50.0% |
| TCAACACGAGCATAGGAGGA+TGG | - | chr4_4:75379545-75379564 | Msa0703460:CDS | 50.0% | |
| ACGAACCCGCATACTATCCG+GGG | - | chr4_4:75379340-75379359 | Msa0703460:CDS | 55.0% | |
| TGATGCCCCGGATAGTATGC+GGG | + | chr4_4:75379348-75379367 | None:intergenic | 55.0% | |
| GTGATGCCCCGGATAGTATG+CGG | + | chr4_4:75379349-75379368 | None:intergenic | 55.0% | |
| !!! | CGCTTTTGATGGTGATGCCC+CGG | + | chr4_4:75379360-75379379 | None:intergenic | 55.0% |
| AGACCGATCGACGAGGTTTG+AGG | - | chr4_4:75379460-75379479 | Msa0703460:CDS | 55.0% | |
| GAGACTGAGACCGATCGACG+AGG | - | chr4_4:75379453-75379472 | Msa0703460:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 75379282 | 75379605 | 75379282 | ID=Msa0703460;Name=Msa0703460 |
| chr4_4 | mRNA | 75379282 | 75379605 | 75379282 | ID=Msa0703460-mRNA-1;Parent=Msa0703460;Name=Msa0703460-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|107 |
| chr4_4 | exon | 75379282 | 75379605 | 75379282 | ID=Msa0703460-mRNA-1:exon:23658;Parent=Msa0703460-mRNA-1 |
| chr4_4 | CDS | 75379282 | 75379605 | 75379282 | ID=Msa0703460-mRNA-1:cds;Parent=Msa0703460-mRNA-1 |
| Gene Sequence |
| Protein sequence |
