AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0568000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0568000 MtrunA17_Chr8g0382521 95.035 282 8 3 1 282 1 276 0.0 538
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0568000 AT5G65120.1 34.810 316 146 13 14 282 14 316 2.50e-30 116
Msa0568000 AT5G10110.1 42.446 139 71 3 134 272 180 309 1.54e-29 114

Find 144 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTTGGAGGATAATGTTTA+TGG 0.122405 4_1:-69595100 Msa0568000:three_prime_UTR
GTGAAACTCTATAGAGTAAA+TGG 0.159932 4_1:+69597063 None:intergenic
GTCTTGGCTTTCCATAAAAT+TGG 0.186392 4_1:+69596952 None:intergenic
GGTTCCGCCGCTGGGTAAAC+TGG 0.190489 4_1:+69596730 None:intergenic
AAACCAAGATGGTGAGAATT+TGG 0.220471 4_1:-69596522 Msa0568000:CDS
AGACCTTTATCTTCGATTTC+CGG 0.227985 4_1:-69596863 Msa0568000:CDS
TGAACAAAATCAACCTTCTT+TGG 0.237716 4_1:-69595116 Msa0568000:three_prime_UTR
CGAATCATCGGTTCCAGTTC+CGG 0.247563 4_1:+69596709 None:intergenic
TCATGCATTATGCACCTTAT+TGG 0.263681 4_1:-69594687 Msa0568000:three_prime_UTR
TGTTCATATGCAAAACATAT+TGG 0.269367 4_1:-69595793 Msa0568000:CDS
AAATCTACACAAATACTTCT+AGG 0.270409 4_1:+69595171 None:intergenic
GGCAAACACCCCATCTGATA+TGG 0.276605 4_1:-69594749 Msa0568000:three_prime_UTR
TCCAGTTCCGGTTCCGCCGC+TGG 0.289847 4_1:+69596721 None:intergenic
TCTGGTGGTTCTATGTTTCC+AGG 0.296796 4_1:-69596569 Msa0568000:CDS
TTGGTTCGCGGGAGGGTAAA+AGG 0.297008 4_1:+69597166 None:intergenic
TATTGAACATGATGACAATT+TGG 0.303245 4_1:-69596636 Msa0568000:CDS
ATGTTTATGGGCTTTGTTGC+GGG 0.304148 4_1:-69595087 Msa0568000:three_prime_UTR
ATGGTATTTGCCTCATGAAA+TGG 0.314808 4_1:+69594645 None:intergenic
ACATGCGTCTACAGCATAAA+AGG 0.317327 4_1:+69594889 None:intergenic
TTCACGCGATTCTCAGTGTT+AGG 0.317799 4_1:-69597001 Msa0568000:five_prime_UTR
GATTACTAGCTGGTTATATC+TGG 0.321901 4_1:-69596334 Msa0568000:intron
CTTTGGAGGATAATGTTTAT+GGG 0.326551 4_1:-69595099 Msa0568000:three_prime_UTR
GTTCATATGCAAAACATATT+GGG 0.335860 4_1:-69595792 Msa0568000:CDS
CCCAGCGGCGGAACCGGAAC+TGG 0.355892 4_1:-69596722 Msa0568000:CDS
TTGTGTAGATTTGTTACATT+TGG 0.358897 4_1:-69595160 Msa0568000:three_prime_UTR
TGGTCCGTCGTCGTCGTTGC+CGG 0.366215 4_1:+69596844 None:intergenic
TACTGTATCTGTTGTGAATT+CGG 0.375771 4_1:-69595207 Msa0568000:three_prime_UTR
TGAGGAATTATTGCCTGAAA+AGG 0.388332 4_1:+69596760 None:intergenic
AGTAATCTTACAATCATAAT+AGG 0.389461 4_1:+69595284 None:intergenic
CATCCAAATTCTCACCATCT+TGG 0.392649 4_1:+69596519 None:intergenic
GAAGATAAAGGTCTCTTTGA+TGG 0.394472 4_1:+69596872 None:intergenic
TTTCAAATTATTTGCTCCAT+GGG 0.403609 4_1:-69595020 Msa0568000:three_prime_UTR
TGGTTCTATGTTTCCAGGGT+TGG 0.412354 4_1:-69596564 Msa0568000:CDS
AAATATACCTTTGGTGCAAA+AGG 0.413611 4_1:+69596445 None:intergenic
CACCGACCCACCAGCACATT+GGG 0.417686 4_1:+69596607 None:intergenic
CACAAACCACAAGACCAATA+AGG 0.420870 4_1:+69594673 None:intergenic
AGTTTACCCAGCGGCGGAAC+CGG 0.422323 4_1:-69596728 Msa0568000:CDS
AAAGATATGTCACAACGATA+AGG 0.427603 4_1:-69596807 Msa0568000:CDS
ATTGAACATGATGACAATTT+GGG 0.429114 4_1:-69596635 Msa0568000:CDS
CATAAGGCTTGATTCTAAAT+CGG 0.432090 4_1:+69595836 None:intergenic
CTAGAGGGACTAAACAATAA+AGG 0.438293 4_1:-69595061 Msa0568000:three_prime_UTR
TTACATTTGGCCGTTTCCAT+TGG 0.440169 4_1:-69595147 Msa0568000:three_prime_UTR
ATTGCTAGAGCTGCTGTTGT+TGG 0.450029 4_1:+69595323 None:intergenic
TTTGTTCATATTAGAACCAA+TGG 0.451639 4_1:+69595131 None:intergenic
AAGATATGTCACAACGATAA+GGG 0.453885 4_1:-69596806 Msa0568000:CDS
TGGTATTTGCCTCATGAAAT+GGG 0.453984 4_1:+69594646 None:intergenic
GATGTCAAGAGTAGACTAAC+TGG 0.454313 4_1:-69596293 Msa0568000:intron
TTCAATTTCCCTCATTGCCA+TGG 0.455649 4_1:-69597130 Msa0568000:five_prime_UTR
TACTCTTGACATCCTGGTAA+TGG 0.456519 4_1:+69596303 None:intergenic
TATGCACCTTATTGGTCTTG+TGG 0.456721 4_1:-69594679 Msa0568000:three_prime_UTR
GAGATTTCCACTGATGCATA+AGG 0.458079 4_1:+69595820 None:intergenic
CACCGTCGCTGAAATTGTCT+TGG 0.458165 4_1:+69596936 None:intergenic
TATAGTTCGCTAGCTTGAAC+AGG 0.462080 4_1:-69594944 Msa0568000:three_prime_UTR
CCAGTTCCGGTTCCGCCGCT+GGG 0.463705 4_1:+69596722 None:intergenic
AAATTATTTGCTCCATGGGT+TGG 0.465685 4_1:-69595016 Msa0568000:three_prime_UTR
GTTGGAAGATCTCAGAATGA+AGG 0.466090 4_1:-69596674 Msa0568000:CDS
GCTTGAACAGGAGGTGTGTT+GGG 0.468115 4_1:-69594932 Msa0568000:three_prime_UTR
TCACGCGATTCTCAGTGTTA+GGG 0.469236 4_1:-69597000 Msa0568000:five_prime_UTR
GAATTGAAACACGAAACTAA+TGG 0.470194 4_1:+69595249 None:intergenic
GGGGTGTTTGCCTCATGAAA+TGG 0.473414 4_1:+69594760 None:intergenic
TGAAACTCTATAGAGTAAAT+GGG 0.484942 4_1:+69597064 None:intergenic
TGGTTCGCGGGAGGGTAAAA+GGG 0.486314 4_1:+69597167 None:intergenic
GTGGAACCACCGATTGTGTC+TGG 0.487166 4_1:-69596587 Msa0568000:CDS
ATAAAACATGCCAAGACAAA+TGG 0.493431 4_1:+69594626 None:intergenic
CTTTGTTGCGGGTTCCTAGA+GGG 0.496242 4_1:-69595076 Msa0568000:three_prime_UTR
ACAAAATCAACCTTCTTTGG+AGG 0.496961 4_1:-69595113 Msa0568000:three_prime_UTR
GAGGAATTATTGCCTGAAAA+GGG 0.498330 4_1:+69596761 None:intergenic
AAAATCTGTTTAACAACCAA+TGG 0.503074 4_1:+69594863 None:intergenic
CCACCGACCCACCAGCACAT+TGG 0.503533 4_1:+69596606 None:intergenic
AGATGCGGCCATGGCAATGA+GGG 0.505697 4_1:+69597122 None:intergenic
ACACTGAGAATCGCGTGAAA+GGG 0.506319 4_1:+69597005 None:intergenic
GAGGCAAATACCATTTGTCT+TGG 0.506507 4_1:-69594636 Msa0568000:three_prime_UTR
GTTCCGCCGCTGGGTAAACT+GGG 0.508457 4_1:+69596731 None:intergenic
ACATATTGGGTCATGAAAGT+AGG 0.508962 4_1:-69595779 Msa0568000:CDS
CTTTATTGTTTAGTCCCTCT+AGG 0.512820 4_1:+69595062 None:intergenic
CTGGTGGTTCTATGTTTCCA+GGG 0.514828 4_1:-69596568 Msa0568000:CDS
AACACTGAGAATCGCGTGAA+AGG 0.518926 4_1:+69597004 None:intergenic
TTGGGATCTTCCCAATGTGC+TGG 0.522054 4_1:-69596617 Msa0568000:CDS
GCAAACACCCCATCTGATAT+GGG 0.522105 4_1:-69594748 Msa0568000:three_prime_UTR
GGATCTTCCCAATGTGCTGG+TGG 0.522163 4_1:-69596614 Msa0568000:CDS
GTGTCACGGAGATGCGGCCA+TGG 0.522513 4_1:+69597113 None:intergenic
TTAGTCTACTCTTGACATCC+TGG 0.525193 4_1:+69596297 None:intergenic
TTGCCGGAAATCGAAGATAA+AGG 0.527004 4_1:+69596860 None:intergenic
CATATCTGAGAGAGCGCGGC+GGG 0.528884 4_1:+69596904 None:intergenic
TTGGAAGATCTCAGAATGAA+GGG 0.529344 4_1:-69596673 Msa0568000:CDS
AAAACTATCATTCGAATCAT+CGG 0.533638 4_1:+69596697 None:intergenic
CATATTAGAACCAATGGAAA+CGG 0.533745 4_1:+69595137 None:intergenic
ATAAAACATCCCATATCAGA+TGG 0.534094 4_1:+69594739 None:intergenic
AGCTTGAACAGGAGGTGTGT+TGG 0.534934 4_1:-69594933 Msa0568000:three_prime_UTR
CTGGTCGGATCTCCATTACC+AGG 0.536348 4_1:-69596315 Msa0568000:CDS
CTATGTTTCCAGGGTTGGAA+CGG 0.536811 4_1:-69596559 Msa0568000:CDS
GAAAAGGGTAACTCGGAACA+AGG 0.537152 4_1:+69596776 None:intergenic
TAAACATTATCCTCCAAAGA+AGG 0.538929 4_1:+69595103 None:intergenic
AATGTTTATGGGCTTTGTTG+CGG 0.538930 4_1:-69595088 Msa0568000:three_prime_UTR
AAAGAGATTGGTTCGCGGGA+GGG 0.540312 4_1:+69597159 None:intergenic
GCACATGCTGGTGGTGATGT+TGG 0.540337 4_1:-69596497 Msa0568000:CDS
GCAGAACACCGTTCCAACCC+TGG 0.543491 4_1:+69596551 None:intergenic
CCACAGAGAGAGACTGTCCC+TGG 0.544188 4_1:+69594711 None:intergenic
GAGTCAGAAAGAAGCAAGAT+CGG 0.544651 4_1:-69595931 Msa0568000:CDS
GAGAATTTGGATGCACATGC+TGG 0.545412 4_1:-69596509 Msa0568000:CDS
TTCCCAATGTGCTGGTGGGT+CGG 0.546200 4_1:-69596609 Msa0568000:CDS
AAGATAAAGGTCTCTTTGAT+GGG 0.557432 4_1:+69596873 None:intergenic
TAAAACATCCCATATCAGAT+GGG 0.561998 4_1:+69594740 None:intergenic
AGGCTTGATTCTAAATCGGA+TGG 0.562907 4_1:+69595840 None:intergenic
CCAGGGACAGTCTCTCTCTG+TGG 0.569008 4_1:-69594711 Msa0568000:three_prime_UTR
ATAGAACCACCAGACACAAT+CGG 0.571824 4_1:+69596578 None:intergenic
CAGGGACAGTCTCTCTCTGT+GGG 0.573598 4_1:-69594710 Msa0568000:three_prime_UTR
TTGTGATTTCCCATTTCATG+AGG 0.581586 4_1:-69594655 Msa0568000:three_prime_UTR
CCAATGTGCTGGTGGGTCGG+TGG 0.593665 4_1:-69596606 Msa0568000:CDS
GTGAAAGGGTTTCAAAAGTG+TGG 0.595745 4_1:+69597019 None:intergenic
ATATATTGGTGGGTATGATC+AGG 0.600873 4_1:+69594558 None:intergenic
AGCCAAGACAATTTCAGCGA+CGG 0.606195 4_1:-69596938 Msa0568000:CDS
ACTAGCTGGTTATATCTGGT+CGG 0.607521 4_1:-69596330 Msa0568000:intron
ATAAAGGTCTCTTTGATGGG+TGG 0.611024 4_1:+69596876 None:intergenic
GGGTAAACTGGGTTCTTGTG+AGG 0.611769 4_1:+69596742 None:intergenic
AATCAAGCCTTATGCATCAG+TGG 0.612172 4_1:-69595827 Msa0568000:CDS
TGCTGGTGGTGATGTTGGCG+TGG 0.615038 4_1:-69596492 Msa0568000:CDS
GAGATGCGGCCATGGCAATG+AGG 0.616003 4_1:+69597121 None:intergenic
GCTTTGTTGCGGGTTCCTAG+AGG 0.620983 4_1:-69595077 Msa0568000:three_prime_UTR
ATTGCCTGAAAAGGGTAACT+CGG 0.621070 4_1:+69596769 None:intergenic
AAAAGAGATTGGTTCGCGGG+AGG 0.621429 4_1:+69597158 None:intergenic
GAGAGTGTGTCACGGAGATG+CGG 0.621654 4_1:+69597107 None:intergenic
ATTTCCGGCAACGACGACGA+CGG 0.627077 4_1:-69596848 Msa0568000:CDS
AAGTCATTGAAACCAACCCA+TGG 0.628801 4_1:+69595004 None:intergenic
GATCTTCCCAATGTGCTGGT+GGG 0.638562 4_1:-69596613 Msa0568000:CDS
TTCGCGGGAGGGTAAAAGGG+TGG 0.640230 4_1:+69597170 None:intergenic
AAAACATCCCATATCAGATG+GGG 0.642859 4_1:+69594741 None:intergenic
AGTTCGCTAGCTTGAACAGG+AGG 0.645084 4_1:-69594941 Msa0568000:three_prime_UTR
AATGAAGGGTTTATTGACCG+TGG 0.650468 4_1:-69596659 Msa0568000:CDS
TGTAGACGCATGTAGTCCAT+TGG 0.651903 4_1:-69594879 Msa0568000:three_prime_UTR
TCAGAAAGAAGCAAGATCGG+TGG 0.655563 4_1:-69595928 Msa0568000:CDS
AAAAGGGTAACTCGGAACAA+GGG 0.667155 4_1:+69596777 None:intergenic
TTGAAAGTGAGAGTGTGTCA+CGG 0.682072 4_1:+69597099 None:intergenic
TGTCATCATGTTCAATACCA+CGG 0.682574 4_1:+69596642 None:intergenic
GAACCCAGTTTACCCAGCGG+CGG 0.688543 4_1:-69596734 Msa0568000:CDS
TAGTCATATCTGAGAGAGCG+CGG 0.690778 4_1:+69596900 None:intergenic
CAAATAATTTGAAAACAAAG+CGG 0.699769 4_1:+69595029 None:intergenic
GAACCACCGATTGTGTCTGG+TGG 0.712426 4_1:-69596584 Msa0568000:CDS
AATTTGGATGCACATGCTGG+TGG 0.719457 4_1:-69596506 Msa0568000:CDS
TCATATCTGAGAGAGCGCGG+CGG 0.731681 4_1:+69596903 None:intergenic
GAACCACCAGACACAATCGG+TGG 0.757045 4_1:+69596581 None:intergenic
GCTATCATACATGAACAACA+AGG 0.760092 4_1:-69595873 Msa0568000:CDS
CAAGAACCCAGTTTACCCAG+CGG 0.766443 4_1:-69596737 Msa0568000:CDS
ATCATACATGAACAACAAGG+CGG 0.772123 4_1:-69595870 Msa0568000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTGAAAATTTATATAAAAT+GGG + chr4_1:69596093-69596112 None:intergenic 10.0%
!! ATTGAAAAAAAAAAAGAGAT+TGG + chr4_1:69594621-69594640 None:intergenic 15.0%
!! TTTATATTCAATTATCATAC+TGG - chr4_1:69595740-69595759 Msa0568000:intron 15.0%
!! CCTTGAAAATTTATATAAAA+TGG + chr4_1:69596094-69596113 None:intergenic 15.0%
!!! CCATTTTATATAAATTTTCA+AGG - chr4_1:69596091-69596110 Msa0568000:intron 15.0%
!!! TTTTATCAGAATATTTTGTT+TGG - chr4_1:69596921-69596940 Msa0568000:CDS 15.0%
!!! TTTCTTTAGATCCAATTTTA+TGG - chr4_1:69594802-69594821 Msa0568000:three_prime_UTR 20.0%
!!! ATCTTTTTGAGTAAATTTTC+TGG + chr4_1:69594944-69594963 None:intergenic 20.0%
!!! AATTTTTTAAGCACTTTTGT+AGG - chr4_1:69595511-69595530 Msa0568000:intron 20.0%
!!! AGTCTTTGACATTTTTATAT+TGG - chr4_1:69595627-69595646 Msa0568000:intron 20.0%
!!! GTCTTTGACATTTTTATATT+GGG - chr4_1:69595628-69595647 Msa0568000:intron 20.0%
!!! AAATTTGAAATATTAAGCAC+AGG + chr4_1:69596041-69596060 None:intergenic 20.0%
!! AACAAAATAAAAACAAACCA+GGG + chr4_1:69596322-69596341 None:intergenic 20.0%
!! AAACAAAATAAAAACAAACC+AGG + chr4_1:69596323-69596342 None:intergenic 20.0%
!! AGTAATCTTACAATCATAAT+AGG + chr4_1:69596484-69596503 None:intergenic 20.0%
!! CAAATAATTTGAAAACAAAG+CGG + chr4_1:69596739-69596758 None:intergenic 20.0%
!!! TTTTTAGCAACAAAGATTTT+TGG - chr4_1:69596782-69596801 Msa0568000:CDS 20.0%
! TGAAACTCTATAGAGTAAAT+GGG + chr4_1:69594704-69594723 None:intergenic 25.0%
! AAAACTATCATTCGAATCAT+CGG + chr4_1:69595071-69595090 None:intergenic 25.0%
! TATTGAACATGATGACAATT+TGG - chr4_1:69595129-69595148 Msa0568000:three_prime_UTR 25.0%
! ATTGAACATGATGACAATTT+GGG - chr4_1:69595130-69595149 Msa0568000:three_prime_UTR 25.0%
! TTTGCGTAAAAATATACCTT+TGG + chr4_1:69595332-69595351 None:intergenic 25.0%
!! AATTTATGTTGATTACTAGC+TGG - chr4_1:69595421-69595440 Msa0568000:intron 25.0%
!!! TTTTATGTTGTGGACTAAAT+TGG + chr4_1:69595595-69595614 None:intergenic 25.0%
!! AAAAATGTCAAAGACTAGTT+CGG + chr4_1:69595623-69595642 None:intergenic 25.0%
! ATATTCAATTATCATACTGG+AGG - chr4_1:69595743-69595762 Msa0568000:intron 25.0%
!! CGTTTATTCCAGAAAATTTT+TGG + chr4_1:69595864-69595883 None:intergenic 25.0%
! TGTTCATATGCAAAACATAT+TGG - chr4_1:69595972-69595991 Msa0568000:intron 25.0%
! GTTCATATGCAAAACATATT+GGG - chr4_1:69595973-69595992 Msa0568000:intron 25.0%
! TCCTTGATATTGCTAAAAAT+TGG - chr4_1:69596121-69596140 Msa0568000:intron 25.0%
! AAATCTACACAAATACTTCT+AGG + chr4_1:69596597-69596616 None:intergenic 25.0%
!!! TTGTGTAGATTTGTTACATT+TGG - chr4_1:69596605-69596624 Msa0568000:CDS 25.0%
! TTTGTTCATATTAGAACCAA+TGG + chr4_1:69596637-69596656 None:intergenic 25.0%
!! TTTTCAAATTATTTGCTCCA+TGG - chr4_1:69596744-69596763 Msa0568000:CDS 25.0%
! TTTCAAATTATTTGCTCCAT+GGG - chr4_1:69596745-69596764 Msa0568000:CDS 25.0%
! AAAATCTGTTTAACAACCAA+TGG + chr4_1:69596905-69596924 None:intergenic 25.0%
!! AAAAAAAAGAGATTGGTTCG+CGG + chr4_1:69594614-69594633 None:intergenic 30.0%
GTGAAACTCTATAGAGTAAA+TGG + chr4_1:69594705-69594724 None:intergenic 30.0%
AAGATAAAGGTCTCTTTGAT+GGG + chr4_1:69594895-69594914 None:intergenic 30.0%
AAAGATATGTCACAACGATA+AGG - chr4_1:69594958-69594977 Msa0568000:three_prime_UTR 30.0%
AAGATATGTCACAACGATAA+GGG - chr4_1:69594959-69594978 Msa0568000:three_prime_UTR 30.0%
!!! GATTCGAATGATAGTTTTGT+TGG - chr4_1:69595073-69595092 Msa0568000:three_prime_UTR 30.0%
! AAATATACCTTTGGTGCAAA+AGG + chr4_1:69595323-69595342 None:intergenic 30.0%
!!! TTCGGGTTTATTTTATGTTG+TGG + chr4_1:69595605-69595624 None:intergenic 30.0%
! AAAATGTCAAAGACTAGTTC+GGG + chr4_1:69595622-69595641 None:intergenic 30.0%
AAAATTGTGAGAGACCAAAT+TGG - chr4_1:69595705-69595724 Msa0568000:intron 30.0%
AATAAAGTGAAGAGCCAATT+TGG + chr4_1:69595722-69595741 None:intergenic 30.0%
TTAGAGCTAGCAAAAAGTTT+AGG + chr4_1:69595810-69595829 None:intergenic 30.0%
!!! TAAACTTTTTGCTAGCTCTA+AGG - chr4_1:69595809-69595828 Msa0568000:CDS 30.0%
CATAAGGCTTGATTCTAAAT+CGG + chr4_1:69595932-69595951 None:intergenic 30.0%
!!! GCCAATTTTTAGCAATATCA+AGG + chr4_1:69596125-69596144 None:intergenic 30.0%
CTAAAAATTGGCGTCAATTA+CGG - chr4_1:69596133-69596152 Msa0568000:intron 30.0%
!! ATTGATAGGCAAAAGTATCA+GGG + chr4_1:69596219-69596238 None:intergenic 30.0%
CAAAAAAAGGTTCCATTGAT+AGG + chr4_1:69596233-69596252 None:intergenic 30.0%
!!! CTATCAATGGAACCTTTTTT+TGG - chr4_1:69596231-69596250 Msa0568000:intron 30.0%
ATGATCCATATGCCAAAAAA+AGG + chr4_1:69596246-69596265 None:intergenic 30.0%
!! GGAAAACACTCAAAAGTATT+AGG + chr4_1:69596346-69596365 None:intergenic 30.0%
GAATTGAAACACGAAACTAA+TGG + chr4_1:69596519-69596538 None:intergenic 30.0%
TACTGTATCTGTTGTGAATT+CGG - chr4_1:69596558-69596577 Msa0568000:CDS 30.0%
CATATTAGAACCAATGGAAA+CGG + chr4_1:69596631-69596650 None:intergenic 30.0%
TGAACAAAATCAACCTTCTT+TGG - chr4_1:69596649-69596668 Msa0568000:CDS 30.0%
TAAACATTATCCTCCAAAGA+AGG + chr4_1:69596665-69596684 None:intergenic 30.0%
! TCTTTGGAGGATAATGTTTA+TGG - chr4_1:69596665-69596684 Msa0568000:CDS 30.0%
! CTTTGGAGGATAATGTTTAT+GGG - chr4_1:69596666-69596685 Msa0568000:CDS 30.0%
!! TTTTGTTTGGTAGCAATCAT+CGG - chr4_1:69596934-69596953 Msa0568000:CDS 30.0%
! TTGTGATTTTCCATTTCATG+AGG - chr4_1:69596995-69597014 Msa0568000:five_prime_UTR 30.0%
TAAAACATCCCATATCAGAT+GGG + chr4_1:69597028-69597047 None:intergenic 30.0%
ATAAAACATCCCATATCAGA+TGG + chr4_1:69597029-69597048 None:intergenic 30.0%
ATAAAACATGCCAAGACAAA+TGG + chr4_1:69597142-69597161 None:intergenic 30.0%
!!! GTCTTGGCATGTTTTATTTT+AGG - chr4_1:69597145-69597164 Msa0568000:five_prime_UTR 30.0%
!! AAAAAAAGAGATTGGTTCGC+GGG + chr4_1:69594613-69594632 None:intergenic 35.0%
! GTCTTGGCTTTCCATAAAAT+TGG + chr4_1:69594816-69594835 None:intergenic 35.0%
GAAGATAAAGGTCTCTTTGA+TGG + chr4_1:69594896-69594915 None:intergenic 35.0%
AGACCTTTATCTTCGATTTC+CGG - chr4_1:69594902-69594921 Msa0568000:three_prime_UTR 35.0%
GAGGAATTATTGCCTGAAAA+GGG + chr4_1:69595007-69595026 None:intergenic 35.0%
TGAGGAATTATTGCCTGAAA+AGG + chr4_1:69595008-69595027 None:intergenic 35.0%
TTGGAAGATCTCAGAATGAA+GGG - chr4_1:69595092-69595111 Msa0568000:three_prime_UTR 35.0%
TGTCATCATGTTCAATACCA+CGG + chr4_1:69595126-69595145 None:intergenic 35.0%
TCTGCTCTTAAAAACCAAGA+TGG - chr4_1:69595232-69595251 Msa0568000:three_prime_UTR 35.0%
AAACCAAGATGGTGAGAATT+TGG - chr4_1:69595243-69595262 Msa0568000:three_prime_UTR 35.0%
GATTACTAGCTGGTTATATC+TGG - chr4_1:69595431-69595450 Msa0568000:intron 35.0%
AAATTGTGCGTGTCCAAATT+GGG + chr4_1:69595564-69595583 None:intergenic 35.0%
AAAATTGTGCGTGTCCAAAT+TGG + chr4_1:69595565-69595584 None:intergenic 35.0%
CTCTCTTTGTCGTAATGAAT+CGG - chr4_1:69595659-69595678 Msa0568000:intron 35.0%
TCTCTTTGTCGTAATGAATC+GGG - chr4_1:69595660-69595679 Msa0568000:intron 35.0%
TGAATCGGGGACTAATTAAT+TGG - chr4_1:69595674-69595693 Msa0568000:intron 35.0%
!!! GTCTCTCACAATTTTTTCTC+AGG + chr4_1:69595700-69595719 None:intergenic 35.0%
GCTATCATACATGAACAACA+AGG - chr4_1:69595892-69595911 Msa0568000:CDS 35.0%
ATCATACATGAACAACAAGG+CGG - chr4_1:69595895-69595914 Msa0568000:CDS 35.0%
ACATATTGGGTCATGAAAGT+AGG - chr4_1:69595986-69596005 Msa0568000:intron 35.0%
ATCAGGGTCAAGGTATATTA+TGG + chr4_1:69596203-69596222 None:intergenic 35.0%
!! CATTGATAGGCAAAAGTATC+AGG + chr4_1:69596220-69596239 None:intergenic 35.0%
! CTGATACTTTTGCCTATCAA+TGG - chr4_1:69596218-69596237 Msa0568000:intron 35.0%
!!! TGGAACCTTTTTTTGGCATA+TGG - chr4_1:69596238-69596257 Msa0568000:intron 35.0%
ACAAAATCAACCTTCTTTGG+AGG - chr4_1:69596652-69596671 Msa0568000:CDS 35.0%
!! AATGTTTATGGGCTTTGTTG+CGG - chr4_1:69596677-69596696 Msa0568000:CDS 35.0%
CTTTATTGTTTAGTCCCTCT+AGG + chr4_1:69596706-69596725 None:intergenic 35.0%
CTAGAGGGACTAAACAATAA+AGG - chr4_1:69596704-69596723 Msa0568000:CDS 35.0%
AAATTATTTGCTCCATGGGT+TGG - chr4_1:69596749-69596768 Msa0568000:CDS 35.0%
AAAACATCCCATATCAGATG+GGG + chr4_1:69597027-69597046 None:intergenic 35.0%
!!! TGGGATGTTTTATTTTAGCC+AGG - chr4_1:69597036-69597055 Msa0568000:five_prime_UTR 35.0%
!!! GGGATGTTTTATTTTAGCCA+GGG - chr4_1:69597037-69597056 Msa0568000:five_prime_UTR 35.0%
TCATGCATTATGCACCTTAT+TGG - chr4_1:69597078-69597097 Msa0568000:five_prime_UTR 35.0%
TTGTGATTTCCCATTTCATG+AGG - chr4_1:69597110-69597129 Msa0568000:five_prime_UTR 35.0%
TGGTATTTGCCTCATGAAAT+GGG + chr4_1:69597122-69597141 None:intergenic 35.0%
ATGGTATTTGCCTCATGAAA+TGG + chr4_1:69597123-69597142 None:intergenic 35.0%
!!! TGGCATGTTTTATTTTAGGC+AGG - chr4_1:69597149-69597168 Msa0568000:five_prime_UTR 35.0%
!!! GGCATGTTTTATTTTAGGCA+GGG - chr4_1:69597150-69597169 Msa0568000:five_prime_UTR 35.0%
TTCAATTTCCCTCATTGCCA+TGG - chr4_1:69594635-69594654 Msa0568000:three_prime_UTR 40.0%
! TTGAAAGTGAGAGTGTGTCA+CGG + chr4_1:69594669-69594688 None:intergenic 40.0%
GTGAAAGGGTTTCAAAAGTG+TGG + chr4_1:69594749-69594768 None:intergenic 40.0%
ATAAAGGTCTCTTTGATGGG+TGG + chr4_1:69594892-69594911 None:intergenic 40.0%
TTGCCGGAAATCGAAGATAA+AGG + chr4_1:69594908-69594927 None:intergenic 40.0%
AAAAGGGTAACTCGGAACAA+GGG + chr4_1:69594991-69595010 None:intergenic 40.0%
ATTGCCTGAAAAGGGTAACT+CGG + chr4_1:69594999-69595018 None:intergenic 40.0%
GTTGGAAGATCTCAGAATGA+AGG - chr4_1:69595091-69595110 Msa0568000:three_prime_UTR 40.0%
AATGAAGGGTTTATTGACCG+TGG - chr4_1:69595106-69595125 Msa0568000:three_prime_UTR 40.0%
ATAGAACCACCAGACACAAT+CGG + chr4_1:69595190-69595209 None:intergenic 40.0%
CATCCAAATTCTCACCATCT+TGG + chr4_1:69595249-69595268 None:intergenic 40.0%
! ACTTGTTCCTTTTGCACCAA+AGG - chr4_1:69595313-69595332 Msa0568000:exon 40.0%
ACTAGCTGGTTATATCTGGT+CGG - chr4_1:69595435-69595454 Msa0568000:intron 40.0%
TACTCTTGACATCCTGGTAA+TGG + chr4_1:69595465-69595484 None:intergenic 40.0%
TTAGTCTACTCTTGACATCC+TGG + chr4_1:69595471-69595490 None:intergenic 40.0%
GATGTCAAGAGTAGACTAAC+TGG - chr4_1:69595472-69595491 Msa0568000:intron 40.0%
! TCAAGAGTGTAGACCCAATT+TGG - chr4_1:69595548-69595567 Msa0568000:intron 40.0%
CTCTTTGTCGTAATGAATCG+GGG - chr4_1:69595661-69595680 Msa0568000:intron 40.0%
GAGTCAGAAAGAAGCAAGAT+CGG - chr4_1:69595834-69595853 Msa0568000:CDS 40.0%
! TCGGTGGTCCAAAAATTTTC+TGG - chr4_1:69595853-69595872 Msa0568000:CDS 40.0%
AGGCTTGATTCTAAATCGGA+TGG + chr4_1:69595928-69595947 None:intergenic 40.0%
! AATCAAGCCTTATGCATCAG+TGG - chr4_1:69595938-69595957 Msa0568000:CDS 40.0%
GAGATTTCCACTGATGCATA+AGG + chr4_1:69595948-69595967 None:intergenic 40.0%
GTATCTGACTACTGCAATTG+AGG + chr4_1:69596168-69596187 None:intergenic 40.0%
TTACATTTGGCCGTTTCCAT+TGG - chr4_1:69596618-69596637 Msa0568000:CDS 40.0%
!! ATGTTTATGGGCTTTGTTGC+GGG - chr4_1:69596678-69596697 Msa0568000:CDS 40.0%
AAGTCATTGAAACCAACCCA+TGG + chr4_1:69596764-69596783 None:intergenic 40.0%
! TATAGTTCGCTAGCTTGAAC+AGG - chr4_1:69596821-69596840 Msa0568000:CDS 40.0%
ACATGCGTCTACAGCATAAA+AGG + chr4_1:69596879-69596898 None:intergenic 40.0%
!! TATGCACCTTATTGGTCTTG+TGG - chr4_1:69597086-69597105 Msa0568000:five_prime_UTR 40.0%
CACAAACCACAAGACCAATA+AGG + chr4_1:69597095-69597114 None:intergenic 40.0%
GAGGCAAATACCATTTGTCT+TGG - chr4_1:69597129-69597148 Msa0568000:five_prime_UTR 40.0%
! AGGGACAGTCTTTTTCTTTC+TGG - chr4_1:69597169-69597188 Msa0568000:five_prime_UTR 40.0%
! GGGACAGTCTTTTTCTTTCT+GGG - chr4_1:69597170-69597189 Msa0568000:five_prime_UTR 40.0%
AAAAAACACACACGGTGAGG+CGG - chr4_1:69594571-69594590 Msa0568000:three_prime_UTR 45.0%
ACACTGAGAATCGCGTGAAA+GGG + chr4_1:69594763-69594782 None:intergenic 45.0%
AACACTGAGAATCGCGTGAA+AGG + chr4_1:69594764-69594783 None:intergenic 45.0%
TTCACGCGATTCTCAGTGTT+AGG - chr4_1:69594764-69594783 Msa0568000:three_prime_UTR 45.0%
TCACGCGATTCTCAGTGTTA+GGG - chr4_1:69594765-69594784 Msa0568000:three_prime_UTR 45.0%
AGCCAAGACAATTTCAGCGA+CGG - chr4_1:69594827-69594846 Msa0568000:three_prime_UTR 45.0%
TAGTCATATCTGAGAGAGCG+CGG + chr4_1:69594868-69594887 None:intergenic 45.0%
GAAAAGGGTAACTCGGAACA+AGG + chr4_1:69594992-69595011 None:intergenic 45.0%
! TGTTCCGAGTTACCCTTTTC+AGG - chr4_1:69594992-69595011 Msa0568000:three_prime_UTR 45.0%
! TCTGGTGGTTCTATGTTTCC+AGG - chr4_1:69595196-69595215 Msa0568000:three_prime_UTR 45.0%
! CTGGTGGTTCTATGTTTCCA+GGG - chr4_1:69595197-69595216 Msa0568000:three_prime_UTR 45.0%
! TGGTTCTATGTTTCCAGGGT+TGG - chr4_1:69595201-69595220 Msa0568000:three_prime_UTR 45.0%
CTATGTTTCCAGGGTTGGAA+CGG - chr4_1:69595206-69595225 Msa0568000:three_prime_UTR 45.0%
GAGAATTTGGATGCACATGC+TGG - chr4_1:69595256-69595275 Msa0568000:three_prime_UTR 45.0%
AATTTGGATGCACATGCTGG+TGG - chr4_1:69595259-69595278 Msa0568000:three_prime_UTR 45.0%
! TGGACACGCACAATTTTCTC+CGG - chr4_1:69595568-69595587 Msa0568000:intron 45.0%
! TGTTGTGGACTAAATTGGCC+CGG + chr4_1:69595590-69595609 None:intergenic 45.0%
TCAGAAAGAAGCAAGATCGG+TGG - chr4_1:69595837-69595856 Msa0568000:CDS 45.0%
AGCACAGGTGATAGATAGCA+CGG + chr4_1:69596026-69596045 None:intergenic 45.0%
!! AGGCAAAAGTATCAGGGTCA+AGG + chr4_1:69596213-69596232 None:intergenic 45.0%
!! GTTTTCCTTCTGCTGCTTCT+AGG - chr4_1:69596359-69596378 Msa0568000:intron 45.0%
TGATTCCTAGAAGCAGCAGA+AGG + chr4_1:69596367-69596386 None:intergenic 45.0%
ATTGCTAGAGCTGCTGTTGT+TGG + chr4_1:69596445-69596464 None:intergenic 45.0%
TGTAGACGCATGTAGTCCAT+TGG - chr4_1:69596886-69596905 Msa0568000:CDS 45.0%
GCAAACACCCCATCTGATAT+GGG - chr4_1:69597017-69597036 Msa0568000:five_prime_UTR 45.0%
AAAAACACACACGGTGAGGC+GGG - chr4_1:69594572-69594591 Msa0568000:three_prime_UTR 50.0%
!! AAAGAGATTGGTTCGCGGGA+GGG + chr4_1:69594609-69594628 None:intergenic 50.0%
!! AAAAGAGATTGGTTCGCGGG+AGG + chr4_1:69594610-69594629 None:intergenic 50.0%
CACCGTCGCTGAAATTGTCT+TGG + chr4_1:69594832-69594851 None:intergenic 50.0%
GGGTAAACTGGGTTCTTGTG+AGG + chr4_1:69595026-69595045 None:intergenic 50.0%
CAAGAACCCAGTTTACCCAG+CGG - chr4_1:69595028-69595047 Msa0568000:three_prime_UTR 50.0%
!! CGAATCATCGGTTCCAGTTC+CGG + chr4_1:69595059-69595078 None:intergenic 50.0%
TTGGGATCTTCCCAATGTGC+TGG - chr4_1:69595148-69595167 Msa0568000:three_prime_UTR 50.0%
! GATCTTCCCAATGTGCTGGT+GGG - chr4_1:69595152-69595171 Msa0568000:three_prime_UTR 50.0%
! GGACACGCACAATTTTCTCC+GGG - chr4_1:69595569-69595588 Msa0568000:intron 50.0%
GTCTTCACTGACATGTACCC+TGG - chr4_1:69596302-69596321 Msa0568000:CDS 50.0%
CTTTGTTGCGGGTTCCTAGA+GGG - chr4_1:69596689-69596708 Msa0568000:CDS 50.0%
! AGTTCGCTAGCTTGAACAGG+AGG - chr4_1:69596824-69596843 Msa0568000:CDS 50.0%
! AGCTTGAACAGGAGGTGTGT+TGG - chr4_1:69596832-69596851 Msa0568000:CDS 50.0%
! GCTTGAACAGGAGGTGTGTT+GGG - chr4_1:69596833-69596852 Msa0568000:CDS 50.0%
! GGGGTGTTTGCCTCATGAAA+TGG + chr4_1:69597008-69597027 None:intergenic 50.0%
GGCAAACACCCCATCTGATA+TGG - chr4_1:69597016-69597035 Msa0568000:five_prime_UTR 50.0%
TGGTTCGCGGGAGGGTAAAA+GGG + chr4_1:69594601-69594620 None:intergenic 55.0%
! TTGGTTCGCGGGAGGGTAAA+AGG + chr4_1:69594602-69594621 None:intergenic 55.0%
AGATGCGGCCATGGCAATGA+GGG + chr4_1:69594646-69594665 None:intergenic 55.0%
! GAGAGTGTGTCACGGAGATG+CGG + chr4_1:69594661-69594680 None:intergenic 55.0%
! TCATATCTGAGAGAGCGCGG+CGG + chr4_1:69594865-69594884 None:intergenic 55.0%
ATTTCCGGCAACGACGACGA+CGG - chr4_1:69594917-69594936 Msa0568000:three_prime_UTR 55.0%
GGATCTTCCCAATGTGCTGG+TGG - chr4_1:69595151-69595170 Msa0568000:three_prime_UTR 55.0%
! TTCCCAATGTGCTGGTGGGT+CGG - chr4_1:69595156-69595175 Msa0568000:three_prime_UTR 55.0%
! GTGGAACCACCGATTGTGTC+TGG - chr4_1:69595178-69595197 Msa0568000:three_prime_UTR 55.0%
! GAACCACCGATTGTGTCTGG+TGG - chr4_1:69595181-69595200 Msa0568000:three_prime_UTR 55.0%
GAACCACCAGACACAATCGG+TGG + chr4_1:69595187-69595206 None:intergenic 55.0%
! GCACATGCTGGTGGTGATGT+TGG - chr4_1:69595268-69595287 Msa0568000:three_prime_UTR 55.0%
CTGGTCGGATCTCCATTACC+AGG - chr4_1:69595450-69595469 Msa0568000:intron 55.0%
! GCTTTGTTGCGGGTTCCTAG+AGG - chr4_1:69596688-69596707 Msa0568000:CDS 55.0%
CAGGGACAGTCTCTCTCTGT+GGG - chr4_1:69597055-69597074 Msa0568000:five_prime_UTR 55.0%
TTCGCGGGAGGGTAAAAGGG+TGG + chr4_1:69594598-69594617 None:intergenic 60.0%
GAGATGCGGCCATGGCAATG+AGG + chr4_1:69594647-69594666 None:intergenic 60.0%
! CATATCTGAGAGAGCGCGGC+GGG + chr4_1:69594864-69594883 None:intergenic 60.0%
GAACCCAGTTTACCCAGCGG+CGG - chr4_1:69595031-69595050 Msa0568000:three_prime_UTR 60.0%
GTTCCGCCGCTGGGTAAACT+GGG + chr4_1:69595037-69595056 None:intergenic 60.0%
AGTTTACCCAGCGGCGGAAC+CGG - chr4_1:69595037-69595056 Msa0568000:three_prime_UTR 60.0%
CACCGACCCACCAGCACATT+GGG + chr4_1:69595161-69595180 None:intergenic 60.0%
GCAGAACACCGTTCCAACCC+TGG + chr4_1:69595217-69595236 None:intergenic 60.0%
! TGCTGGTGGTGATGTTGGCG+TGG - chr4_1:69595273-69595292 Msa0568000:three_prime_UTR 60.0%
CCACAGAGAGAGACTGTCCC+TGG + chr4_1:69597057-69597076 None:intergenic 60.0%
CCAGGGACAGTCTCTCTCTG+TGG - chr4_1:69597054-69597073 Msa0568000:five_prime_UTR 60.0%
GTGTCACGGAGATGCGGCCA+TGG + chr4_1:69594655-69594674 None:intergenic 65.0%
TGGTCCGTCGTCGTCGTTGC+CGG + chr4_1:69594924-69594943 None:intergenic 65.0%
GGTTCCGCCGCTGGGTAAAC+TGG + chr4_1:69595038-69595057 None:intergenic 65.0%
CCACCGACCCACCAGCACAT+TGG + chr4_1:69595162-69595181 None:intergenic 65.0%
! CCAATGTGCTGGTGGGTCGG+TGG - chr4_1:69595159-69595178 Msa0568000:three_prime_UTR 65.0%
CCAGTTCCGGTTCCGCCGCT+GGG + chr4_1:69595046-69595065 None:intergenic 70.0%
TCCAGTTCCGGTTCCGCCGC+TGG + chr4_1:69595047-69595066 None:intergenic 70.0%
CCCAGCGGCGGAACCGGAAC+TGG - chr4_1:69595043-69595062 Msa0568000:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 69594570 69597217 69594570 ID=Msa0568000;Name=Msa0568000
chr4_1 mRNA 69594570 69597217 69594570 ID=Msa0568000-mRNA-1;Parent=Msa0568000;Name=Msa0568000-mRNA-1;_AED=0.03;_eAED=0.03;_QI=251|1|1|1|1|1|4|747|282
chr4_1 exon 69594570 69595406 69594570 ID=Msa0568000-mRNA-1:exon:23261;Parent=Msa0568000-mRNA-1
chr4_1 exon 69595773 69595963 69595773 ID=Msa0568000-mRNA-1:exon:23260;Parent=Msa0568000-mRNA-1
chr4_1 exon 69596294 69596347 69596294 ID=Msa0568000-mRNA-1:exon:23259;Parent=Msa0568000-mRNA-1
chr4_1 exon 69596453 69597217 69596453 ID=Msa0568000-mRNA-1:exon:23258;Parent=Msa0568000-mRNA-1
chr4_1 five_prime_UTR 69596967 69597217 69596967 ID=Msa0568000-mRNA-1:five_prime_utr;Parent=Msa0568000-mRNA-1
chr4_1 CDS 69596453 69596966 69596453 ID=Msa0568000-mRNA-1:cds;Parent=Msa0568000-mRNA-1
chr4_1 CDS 69596294 69596347 69596294 ID=Msa0568000-mRNA-1:cds;Parent=Msa0568000-mRNA-1
chr4_1 CDS 69595773 69595963 69595773 ID=Msa0568000-mRNA-1:cds;Parent=Msa0568000-mRNA-1
chr4_1 CDS 69595317 69595406 69595317 ID=Msa0568000-mRNA-1:cds;Parent=Msa0568000-mRNA-1
chr4_1 three_prime_UTR 69594570 69595316 69594570 ID=Msa0568000-mRNA-1:three_prime_utr;Parent=Msa0568000-mRNA-1
Gene Sequence

>Msa0568000

ATGGAAAGCCAAGACAATTTCAGCGACGGTGCTTTCAGAACCCGCCGCGCTCTCTCAGATATGACTAACCACCCATCAAAGAGACCTTTATCTTCGATTTCCGGCAACGACGACGACGGACCAGAAAATTTACTCAAAAAGATATGTCACAACGATAAGGGCAAAAACCCTTGTTCCGAGTTACCCTTTTCAGGCAATAATTCCTCACAAGAACCCAGTTTACCCAGCGGCGGAACCGGAACTGGAACCGATGATTCGAATGATAGTTTTGTTGGAAGATCTCAGAATGAAGGGTTTATTGACCGTGGTATTGAACATGATGACAATTTGGGATCTTCCCAATGTGCTGGTGGGTCGGTGGAACCACCGATTGTGTCTGGTGGTTCTATGTTTCCAGGGTTGGAACGGTGTTCTGCTCTTAAAAACCAAGATGGTGAGAATTTGGATGCACATGCTGGTGGTGATGTTGGCGTGGAATTTCTGAAAAATTGTACTTGTTCCTTTTGCACCAAAGCTGGTTATATCTGGTCGGATCTCCATTACCAGGATGTCAAGAGTAGACTAACTGCTCTAAGGAAGAGTCAGAAAGAAGCAAGATCGGTGGTCCAAAAATTTTCTGGAATAAACGATACTCATGCTATCATACATGAACAACAAGGCGGCATTGATCCATCCGATTTAGAATCAAGCCTTATGCATCAGTGGAAATCTCTGTTTGTTCATATGCAAAACATATTGGGTCATGAAAGTAGGCAACTTGAATCAAGCTTTGAAACACTGAAAGATGTGAGAGAGAACTGCAAGAATGATCTTGAGTTGACCAACAACAGCAGCTCTAGCAATCATTAG
Protein sequence

>Msa0568000

MESQDNFSDGAFRTRRALSDMTNHPSKRPLSSISGNDDDGPENLLKKICHNDKGKNPCSELPFSGNNSSQEPSLPSGGTGTGTDDSNDSFVGRSQNEGFIDRGIEHDDNLGSSQCAGGSVEPPIVSGGSMFPGLERCSALKNQDGENLDAHAGGDVGVEFLKNCTCSFCTKAGYIWSDLHYQDVKSRLTALRKSQKEARSVVQKFSGINDTHAIIHEQQGGIDPSDLESSLMHQWKSLFVHMQNILGHESRQLESSFETLKDVRENCKNDLELTNNSSSSNH*