AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0574590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574590 MtrunA17_Chr5g0447291 80.000 55 11 0 2 56 81 135 5.39e-26 92.8
Msa0574590 MtrunA17_Chr2g0285701 72.727 55 15 0 2 56 78 132 2.29e-22 83.6
Msa0574590 MtrunA17_Chr7g0259371 48.148 54 28 0 4 57 98 151 4.96e-12 57.4
Msa0574590 MtrunA17_Chr2g0310471 48.214 56 28 1 2 56 116 171 6.58e-12 57.8
Msa0574590 MtrunA17_Chr7g0259381 46.296 54 29 0 4 57 84 137 2.77e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574590 AT2G03170.1 45.283 53 29 0 4 56 89 141 8.01e-11 54.3

Find 98 sgRNAs with CRISPR-Local

Find 247 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCACCTCAAATGCATTT+AGG 0.189500 4_1:+78189492 None:intergenic
TTCAGTGATTTACTTGAATA+TGG 0.201160 4_1:-78193469 Msa0574590:CDS
TCAGAGAATGCAGTTTAATT+AGG 0.227209 4_1:+78192361 None:intergenic
GTCATCAAGGACTGCTAATT+TGG 0.240698 4_1:+78189994 None:intergenic
TTGCACCTCAAATGCATTTA+GGG 0.269078 4_1:+78189493 None:intergenic
TATCATGTATTTGTAATATA+TGG 0.301926 4_1:-78189593 Msa0574590:three_prime_UTR
TATCATGCATTTGAAATATA+TGG 0.303930 4_1:-78189640 Msa0574590:three_prime_UTR
GATGACTCCATCGTATTGTT+GGG 0.335570 4_1:+78193729 None:intergenic
TGATGTAATCTTGATATTAT+CGG 0.363331 4_1:-78192341 Msa0574590:three_prime_UTR
GTATTGTTGGGGTTAGAGTT+TGG 0.371516 4_1:+78193741 None:intergenic
TCTTTCTCTTTGCCATCATT+TGG 0.375386 4_1:+78193538 None:intergenic
TGCAGAGTCCATATTGGAAT+TGG 0.380229 4_1:+78193885 None:intergenic
ATTGGAGATGCAAGGTTCAT+TGG 0.383357 4_1:-78192276 Msa0574590:three_prime_UTR
GAATTTCAGGAACAACATAA+TGG 0.391209 4_1:-78190044 Msa0574590:intron
TTGAGGTGCAAAGGTGAAAA+TGG 0.395724 4_1:-78189481 Msa0574590:three_prime_UTR
TCACCTTGATCCACCGCTCC+GGG 0.397483 4_1:+78193302 None:intergenic
TTCTTTGTATAGAATATTAG+TGG 0.411001 4_1:+78189441 None:intergenic
GTCACCTTGATCCACCGCTC+CGG 0.413123 4_1:+78193301 None:intergenic
AAGGTTCATTGGTTCCTTAA+CGG 0.420149 4_1:-78192265 Msa0574590:three_prime_UTR
TGATGACTCCATCGTATTGT+TGG 0.421567 4_1:+78193728 None:intergenic
AGTTTGGACTTCGGAGGATA+GGG 0.427298 4_1:+78193757 None:intergenic
TATGCCAATAAAGAAAATAT+AGG 0.427707 4_1:+78193926 None:intergenic
GTATGCCCTAAATGCATTTG+AGG 0.429746 4_1:-78189498 Msa0574590:three_prime_UTR
GATACGTGCAGAGTCCATAT+TGG 0.430900 4_1:+78193879 None:intergenic
CTACACTCTGCCTCTTCCTC+CGG 0.432291 4_1:+78193345 None:intergenic
CAAAAGAAGAGAGAAGAGAA+GGG 0.435980 4_1:+78193827 None:intergenic
GAGTTTGGACTTCGGAGGAT+AGG 0.439564 4_1:+78193756 None:intergenic
AACCTTGCATCTCCAATCCT+TGG 0.444777 4_1:+78192282 None:intergenic
GCAGCAAGAAATTAATAGTC+TGG 0.445796 4_1:-78192482 Msa0574590:three_prime_UTR
ATGAAAAGTTATTTGTCAAA+GGG 0.455847 4_1:+78193786 None:intergenic
TCTTACATGGATATCCGTTA+AGG 0.465833 4_1:+78192251 None:intergenic
GTCATGAAATTGAACTTAGA+TGG 0.468107 4_1:-78189806 Msa0574590:three_prime_UTR
GATGAAAAGTTATTTGTCAA+AGG 0.470505 4_1:+78193785 None:intergenic
GATATATAAACGCAAATATG+TGG 0.477146 4_1:+78189674 None:intergenic
TTCCGTCGAGGAACTGAAGA+AGG 0.477660 4_1:-78193512 Msa0574590:five_prime_UTR
ACAAAAGAAGAGAGAAGAGA+AGG 0.486828 4_1:+78193826 None:intergenic
TGGAACACAATTCCAAGGAT+TGG 0.491650 4_1:-78192294 Msa0574590:three_prime_UTR
ATCGAAGATGCTGGTGGAAG+TGG 0.492902 4_1:-78192314 Msa0574590:three_prime_UTR
GGGTATGTGAGAAAGTATTT+CGG 0.493768 4_1:-78193387 Msa0574590:CDS
AAGGATTGCAGAATTTCAGT+TGG 0.497742 4_1:-78191208 Msa0574590:three_prime_UTR
ATGTAACTTTATTTCAAACC+TGG 0.500216 4_1:-78192511 Msa0574590:exon
GGATCAAGAATAAAAGCGTT+GGG 0.504221 4_1:-78193407 Msa0574590:CDS
CCATGGTTGAATGTGAAGTA+AGG 0.507997 4_1:-78191227 Msa0574590:three_prime_UTR
TAGCACGGCATGGTTCGTAC+TGG 0.513549 4_1:+78189896 None:intergenic
TTCATGTTCTTGGTGAGAGT+TGG 0.514925 4_1:+78193657 None:intergenic
CGGATCAAGAATAAAAGCGT+TGG 0.520127 4_1:-78193408 Msa0574590:CDS
GATCATCGAATACCAAATGA+TGG 0.523334 4_1:-78193550 Msa0574590:five_prime_UTR
GTGTCTGAAGCTGGTCATCA+AGG 0.531764 4_1:+78189981 None:intergenic
GTTTCTGACTGAGGTTGTTG+TGG 0.531819 4_1:-78193433 Msa0574590:CDS
GATGATCTTGTTGATGTGGC+TGG 0.532606 4_1:+78193566 None:intergenic
GCATTGGTTCATTCCTTGCG+TGG 0.536394 4_1:-78189829 Msa0574590:three_prime_UTR
ATTTCTTGCTGCCTACCACC+AGG 0.544336 4_1:+78192493 None:intergenic
TTCAGGAACAACATAATGGA+TGG 0.545973 4_1:-78190040 Msa0574590:intron
ATCTCTCTCAAAACCTCAGA+CGG 0.546220 4_1:-78193699 Msa0574590:five_prime_UTR
TGACTGAGGTTGTTGTGGAT+CGG 0.547463 4_1:-78193428 Msa0574590:CDS
CATTGGTTCATTCCTTGCGT+GGG 0.547592 4_1:-78189828 Msa0574590:three_prime_UTR
CTGCTAATTTGGCTCAAAGT+GGG 0.548274 4_1:+78190005 None:intergenic
ATGACTCCATCGTATTGTTG+GGG 0.548299 4_1:+78193730 None:intergenic
GGTCATTATGTGTCTGAAGC+TGG 0.552073 4_1:+78189972 None:intergenic
TTTAAAAGATTAGCACGGCA+TGG 0.561739 4_1:+78189886 None:intergenic
AAAATGGAGGAGAAAGGGGT+GGG 0.563879 4_1:+78193852 None:intergenic
TGTAATATATGGCAAGTATG+TGG 0.564078 4_1:-78189582 Msa0574590:three_prime_UTR
AACGTTGAGGAGAGGAATGA+CGG 0.567879 4_1:+78193593 None:intergenic
CACCTTGATCCACCGCTCCG+GGG 0.571687 4_1:+78193303 None:intergenic
CCTTACTTCACATTCAACCA+TGG 0.572791 4_1:+78191227 None:intergenic
GGAGAGGAATGACGGAGACG+TGG 0.575899 4_1:+78193601 None:intergenic
ATCCTTCTTCAGTTCCTCGA+CGG 0.577496 4_1:+78193510 None:intergenic
CAATAAAGAAAATATAGGCT+TGG 0.578683 4_1:+78193931 None:intergenic
ACCTTGATCCACCGCTCCGG+GGG 0.584692 4_1:+78193304 None:intergenic
TTCCAAGGATTGGAGATGCA+AGG 0.587603 4_1:-78192284 Msa0574590:three_prime_UTR
TGGGGTTAGAGTTTGGACTT+CGG 0.591201 4_1:+78193748 None:intergenic
GATAATTTAAAAGATTAGCA+CGG 0.593168 4_1:+78189881 None:intergenic
TCAATTTCATGACCCACGCA+AGG 0.594107 4_1:+78189816 None:intergenic
ACTGTGTGCCTTCAAACCCC+CGG 0.596255 4_1:-78193320 Msa0574590:CDS
ATATATAAACGCAAATATGT+GGG 0.596880 4_1:+78189675 None:intergenic
ATCGGATGGATCGAAGATGC+TGG 0.597079 4_1:-78192323 Msa0574590:three_prime_UTR
GTTTGGACTTCGGAGGATAG+GGG 0.597686 4_1:+78193758 None:intergenic
ACTGCTAATTTGGCTCAAAG+TGG 0.598326 4_1:+78190004 None:intergenic
GTTCTTGGTGAGAGTTGGTG+AGG 0.599357 4_1:+78193662 None:intergenic
TAAATGCATTTGAGGTGCAA+AGG 0.603823 4_1:-78189490 Msa0574590:three_prime_UTR
GAGAGGAATGACGGAGACGT+GGG 0.604113 4_1:+78193602 None:intergenic
TTTATTTCAAACCTGGTGGT+AGG 0.605476 4_1:-78192504 Msa0574590:three_prime_UTR
CGGAGGAAGAGGCAGAGTGT+AGG 0.610944 4_1:-78193344 Msa0574590:CDS
ATCTTGTTGATGTGGCTGGA+GGG 0.612025 4_1:+78193570 None:intergenic
GTAATCTTGATATTATCGGA+TGG 0.620607 4_1:-78192337 Msa0574590:three_prime_UTR
CTGGAGGGAACGTTGAGGAG+AGG 0.623739 4_1:+78193585 None:intergenic
GGAAGTGGAACACAATTCCA+AGG 0.626464 4_1:-78192299 Msa0574590:three_prime_UTR
ATTCGATGATCTTGTTGATG+TGG 0.627758 4_1:+78193562 None:intergenic
CTCTAACCCCAACAATACGA+TGG 0.635076 4_1:-78193736 Msa0574590:five_prime_UTR
GCATGCAATTCATGAAGCCA+TGG 0.636367 4_1:-78191244 Msa0574590:three_prime_UTR
GGTTAGAGTTTGGACTTCGG+AGG 0.640644 4_1:+78193751 None:intergenic
TAACTTTATTTCAAACCTGG+TGG 0.641501 4_1:-78192508 Msa0574590:three_prime_UTR
GATCTTGTTGATGTGGCTGG+AGG 0.641617 4_1:+78193569 None:intergenic
GGATGGATCGAAGATGCTGG+TGG 0.648917 4_1:-78192320 Msa0574590:three_prime_UTR
GCAAGAAATTAATAGTCTGG+TGG 0.650097 4_1:-78192479 Msa0574590:intron
GTATTCTATCAAGTCTTCAG+TGG 0.655763 4_1:+78189951 None:intergenic
TGAAATATATGGCAAGTGTG+TGG 0.717534 4_1:-78189629 Msa0574590:three_prime_UTR
TGTGGCTGGAGGGAACGTTG+AGG 0.718641 4_1:+78193580 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTAAATAGAAAAACATAA+AGG + chr4_1:78190848-78190867 None:intergenic 10.0%
!!! TACATTCTAAAATAAAATTT+GGG + chr4_1:78191449-78191468 None:intergenic 10.0%
!!! TTACATTCTAAAATAAAATT+TGG + chr4_1:78191450-78191469 None:intergenic 10.0%
!!! GTAAAGTTTTTTTTTTTTTT+AGG - chr4_1:78193551-78193570 Msa0574590:five_prime_UTR 10.0%
!!! ATAAGTTCAAAATCTTTTAA+AGG - chr4_1:78190880-78190899 Msa0574590:intron 15.0%
!!! ATATAGTTTTAAAACTTAAG+AGG + chr4_1:78191127-78191146 None:intergenic 15.0%
!! TATTATTCCACTTATATATT+TGG - chr4_1:78191144-78191163 Msa0574590:intron 15.0%
!! AAAAAAACCAAATATATAAG+TGG + chr4_1:78191154-78191173 None:intergenic 15.0%
!!! ACATTCTAAAATAAAATTTG+GGG + chr4_1:78191448-78191467 None:intergenic 15.0%
!!! ATAAATTGATTGATTTTTCT+TGG - chr4_1:78191894-78191913 Msa0574590:intron 15.0%
!! CAGCATAAAATATAAAAAAT+TGG - chr4_1:78192626-78192645 Msa0574590:intron 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr4_1:78192893-78192912 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr4_1:78192892-78192911 Msa0574590:intron 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - chr4_1:78192916-78192935 Msa0574590:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr4_1:78193019-78193038 Msa0574590:intron 15.0%
!!! AATCTTAAATTTTGAATTTC+AGG - chr4_1:78193214-78193233 Msa0574590:intron 15.0%
!! TATCATGTATTTGTAATATA+TGG - chr4_1:78193678-78193697 Msa0574590:five_prime_UTR 15.0%
!! TATGCCAATAAAGAAAATAT+AGG + chr4_1:78189348-78189367 None:intergenic 20.0%
!! ATGAAAAGTTATTTGTCAAA+GGG + chr4_1:78189488-78189507 None:intergenic 20.0%
!!! TGTTGAAAATGATTTTAATC+CGG - chr4_1:78189907-78189926 Msa0574590:three_prime_UTR 20.0%
!!! AAAAAAATCACTTTAGAACA+GGG + chr4_1:78189995-78190014 None:intergenic 20.0%
!!! AAAAAAAATCACTTTAGAAC+AGG + chr4_1:78189996-78190015 None:intergenic 20.0%
!! CTAGCAAAAAAAAAAATCAA+TGG + chr4_1:78190099-78190118 None:intergenic 20.0%
!! TTTAATGTGTAAACTTCTTA+AGG - chr4_1:78190662-78190681 Msa0574590:intron 20.0%
!!! TTCAAAATCTTTTAAAGGTT+TGG - chr4_1:78190885-78190904 Msa0574590:intron 20.0%
!! TGATGTAATCTTGATATTAT+CGG - chr4_1:78190930-78190949 Msa0574590:intron 20.0%
!! TAATCACACAATTAAAAAAG+AGG + chr4_1:78191058-78191077 None:intergenic 20.0%
!!! AACTTTGTTTGAATTTGTTT+TGG - chr4_1:78191081-78191100 Msa0574590:intron 20.0%
!! ACTTCGTAAAATATTTAGTA+TGG + chr4_1:78191309-78191328 None:intergenic 20.0%
!!! CTTTAATTGTTTTCTGTTAT+TGG - chr4_1:78191564-78191583 Msa0574590:intron 20.0%
!! AGATATAATGTTCTCATTTA+TGG - chr4_1:78191967-78191986 Msa0574590:intron 20.0%
!!! AAAGAGTATATAATCATATG+AGG + chr4_1:78192277-78192296 None:intergenic 20.0%
!!! TTTATGTCATTTTTGTTAGA+AGG - chr4_1:78192487-78192506 Msa0574590:three_prime_UTR 20.0%
!!! ACTTATAACTATAAGTTGAT+TGG - chr4_1:78192681-78192700 Msa0574590:intron 20.0%
!! AATATTAGAATATGTACATC+TGG + chr4_1:78192779-78192798 None:intergenic 20.0%
!! AATGATTAAAAGAAAGAGAA+TGG - chr4_1:78192859-78192878 Msa0574590:intron 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr4_1:78192902-78192921 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr4_1:78192979-78192998 Msa0574590:intron 20.0%
!! TATTGATCTTATCAATTGTA+TGG + chr4_1:78193191-78193210 None:intergenic 20.0%
!! GATAATTTAAAAGATTAGCA+CGG + chr4_1:78193393-78193412 None:intergenic 20.0%
!! ATATATAAACGCAAATATGT+GGG + chr4_1:78193599-78193618 None:intergenic 20.0%
!!! TATCATGCATTTGAAATATA+TGG - chr4_1:78193631-78193650 Msa0574590:five_prime_UTR 20.0%
!!! AAGTTTCTTTTAGAATTTGT+TGG - chr4_1:78193731-78193750 Msa0574590:five_prime_UTR 20.0%
!! TTCTTTGTATAGAATATTAG+TGG + chr4_1:78193833-78193852 None:intergenic 20.0%
! CAATAAAGAAAATATAGGCT+TGG + chr4_1:78189343-78189362 None:intergenic 25.0%
!! CAAGCCTATATTTTCTTTAT+TGG - chr4_1:78189341-78189360 Msa0574590:three_prime_UTR 25.0%
!! ATTTTTCTCCAATTCCAATA+TGG - chr4_1:78189378-78189397 Msa0574590:three_prime_UTR 25.0%
! GATGAAAAGTTATTTGTCAA+AGG + chr4_1:78189489-78189508 None:intergenic 25.0%
!! TTGAATAGTTTTCATGTTCT+TGG + chr4_1:78189627-78189646 None:intergenic 25.0%
! TTCAGTGATTTACTTGAATA+TGG - chr4_1:78189802-78189821 Msa0574590:three_prime_UTR 25.0%
! TTAGACTATATGTATGTTGT+TGG - chr4_1:78190178-78190197 Msa0574590:intron 25.0%
! ATCTATTTGCAGGAAAAAAA+TGG + chr4_1:78190211-78190230 None:intergenic 25.0%
! ATCTAATTGTTTCAGCAAAT+TGG + chr4_1:78190316-78190335 None:intergenic 25.0%
!! GTAAAAAAGTTTAGTCCTTT+TGG + chr4_1:78190406-78190425 None:intergenic 25.0%
!! ATGTAACTTTATTTCAAACC+TGG - chr4_1:78190760-78190779 Msa0574590:intron 25.0%
! GTCAAATCAAAATTCTTACA+TGG + chr4_1:78191036-78191055 None:intergenic 25.0%
! CGTAAAATATTTAGTATGGT+TGG + chr4_1:78191305-78191324 None:intergenic 25.0%
!!! TTCTAAAATAAAATTTGGGG+TGG + chr4_1:78191445-78191464 None:intergenic 25.0%
! AGTTGTTACATGATAAGAAA+AGG + chr4_1:78191660-78191679 None:intergenic 25.0%
! ATGAATAACTCATTGAAAGA+AGG + chr4_1:78191829-78191848 None:intergenic 25.0%
! TTCAATGAGTTATTCATATG+TGG - chr4_1:78191832-78191851 Msa0574590:intron 25.0%
! TGCATAAATAGAATGATCTA+CGG + chr4_1:78191865-78191884 None:intergenic 25.0%
! AAAAAGCAATAGAAAAAGAC+AGG - chr4_1:78192097-78192116 Msa0574590:intron 25.0%
! AAAAGCAATAGAAAAAGACA+GGG - chr4_1:78192098-78192117 Msa0574590:intron 25.0%
!! GTATAAGTACACAAATCTTT+TGG + chr4_1:78192171-78192190 None:intergenic 25.0%
!! ACATATCTAGCATTTGAAAT+AGG - chr4_1:78192201-78192220 Msa0574590:intron 25.0%
! AACATATCTAGCATTTGAAA+TGG - chr4_1:78192200-78192219 Msa0574590:intron 25.0%
!! ACATATCTAGCATTTGAAAT+GGG - chr4_1:78192201-78192220 Msa0574590:intron 25.0%
! TTTAAGAGTGAAAATTACCA+TGG + chr4_1:78192351-78192370 None:intergenic 25.0%
!! ATGGTAATTTTCACTCTTAA+AGG - chr4_1:78192350-78192369 Msa0574590:three_prime_UTR 25.0%
! TGTTAGAAGGTTACTATAAA+TGG - chr4_1:78192500-78192519 Msa0574590:three_prime_UTR 25.0%
! CTATAAATGGTAAATTCCAA+TGG - chr4_1:78192513-78192532 Msa0574590:exon 25.0%
! AAGACATTAACATATACCAT+TGG + chr4_1:78192532-78192551 None:intergenic 25.0%
! ACTGTCCTATTTAACTATTT+AGG - chr4_1:78192581-78192600 Msa0574590:intron 25.0%
! GGAATCCTAAATAGTTAAAT+AGG + chr4_1:78192589-78192608 None:intergenic 25.0%
!!! GATTGGTTTTACATAATACT+AGG - chr4_1:78192698-78192717 Msa0574590:intron 25.0%
! AATATAAAACACATCAAGGA+GGG + chr4_1:78193090-78193109 None:intergenic 25.0%
! ATGCAATATAAAACACATCA+AGG + chr4_1:78193094-78193113 None:intergenic 25.0%
! CCAACAAAAAATTATTTGGT+TGG + chr4_1:78193415-78193434 None:intergenic 25.0%
!!! CCAACCAAATAATTTTTTGT+TGG - chr4_1:78193412-78193431 Msa0574590:CDS 25.0%
! TCTGCCAACAAAAAATTATT+TGG + chr4_1:78193419-78193438 None:intergenic 25.0%
! GATATATAAACGCAAATATG+TGG + chr4_1:78193600-78193619 None:intergenic 25.0%
!! TGAAAATGATTTTAATCCGG+AGG - chr4_1:78189910-78189929 Msa0574590:three_prime_UTR 30.0%
! TCCAAAAATTTTGCTTGCTT+TGG - chr4_1:78190352-78190371 Msa0574590:intron 30.0%
! TCCAAAGCAAGCAAAATTTT+TGG + chr4_1:78190356-78190375 None:intergenic 30.0%
GTTGTATTCCAAAGCTATAA+AGG - chr4_1:78190436-78190455 Msa0574590:intron 30.0%
ATTGCAAACCTTTATAGCTT+TGG + chr4_1:78190447-78190466 None:intergenic 30.0%
GATTAATTCAAGTCAGTTTG+AGG - chr4_1:78190684-78190703 Msa0574590:intron 30.0%
ATATTTCACCTTTGTCTGTA+GGG - chr4_1:78190735-78190754 Msa0574590:intron 30.0%
! TAACTTTATTTCAAACCTGG+TGG - chr4_1:78190763-78190782 Msa0574590:intron 30.0%
TCAGAGAATGCAGTTTAATT+AGG + chr4_1:78190913-78190932 None:intergenic 30.0%
GTAATCTTGATATTATCGGA+TGG - chr4_1:78190934-78190953 Msa0574590:intron 30.0%
CAAGCATGAGATATAAGAAT+TGG + chr4_1:78191209-78191228 None:intergenic 30.0%
CAATTCTTATATCTCATGCT+TGG - chr4_1:78191207-78191226 Msa0574590:three_prime_UTR 30.0%
GTTGGCAGTATGTAAAAAAT+AGG + chr4_1:78191287-78191306 None:intergenic 30.0%
!! GACTTTAATGTTGGTGTTAT+GGG + chr4_1:78191344-78191363 None:intergenic 30.0%
!! TGACTTTAATGTTGGTGTTA+TGG + chr4_1:78191345-78191364 None:intergenic 30.0%
!! TAAAATTTGGGGTGGTTTTT+GGG + chr4_1:78191437-78191456 None:intergenic 30.0%
!! ATAAAATTTGGGGTGGTTTT+TGG + chr4_1:78191438-78191457 None:intergenic 30.0%
ATTGTGTCCATGATTATTGT+CGG - chr4_1:78191715-78191734 Msa0574590:intron 30.0%
ATGTTCTCATTTATGGTTTC+TGG - chr4_1:78191974-78191993 Msa0574590:intron 30.0%
TCAAATGCTAGATATGTTTG+AGG + chr4_1:78192198-78192217 None:intergenic 30.0%
TCAAATGCTAGATATGTTTG+AGG + chr4_1:78192198-78192217 None:intergenic 30.0%
! TACCATGGATTAAAGCTAAA+AGG + chr4_1:78192336-78192355 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr4_1:78192811-78192830 Msa0574590:intron 30.0%
ATATAAAACACATCAAGGAG+GGG + chr4_1:78193089-78193108 None:intergenic 30.0%
CAATATAAAACACATCAAGG+AGG + chr4_1:78193091-78193110 None:intergenic 30.0%
GAATTTCAGGAACAACATAA+TGG - chr4_1:78193227-78193246 Msa0574590:intron 30.0%
GTCATGAAATTGAACTTAGA+TGG - chr4_1:78193465-78193484 Msa0574590:CDS 30.0%
TGTAATATATGGCAAGTATG+TGG - chr4_1:78193689-78193708 Msa0574590:five_prime_UTR 30.0%
AGAGAAGAGAAGGGTAAAAA+TGG + chr4_1:78189438-78189457 None:intergenic 35.0%
CAAAAGAAGAGAGAAGAGAA+GGG + chr4_1:78189447-78189466 None:intergenic 35.0%
ACAAAAGAAGAGAGAAGAGA+AGG + chr4_1:78189448-78189467 None:intergenic 35.0%
ATTCGATGATCTTGTTGATG+TGG + chr4_1:78189712-78189731 None:intergenic 35.0%
GATCATCGAATACCAAATGA+TGG - chr4_1:78189721-78189740 Msa0574590:three_prime_UTR 35.0%
TCTTTCTCTTTGCCATCATT+TGG + chr4_1:78189736-78189755 None:intergenic 35.0%
!!! TCTTTTTGAGTTTCTGACTG+AGG - chr4_1:78189829-78189848 Msa0574590:three_prime_UTR 35.0%
!! GGATCAAGAATAAAAGCGTT+GGG - chr4_1:78189864-78189883 Msa0574590:three_prime_UTR 35.0%
!! GGGTATGTGAGAAAGTATTT+CGG - chr4_1:78189884-78189903 Msa0574590:three_prime_UTR 35.0%
TTCCTGCAAATAGATGTTGT+CGG - chr4_1:78190216-78190235 Msa0574590:intron 35.0%
TTATGCAAGTCTTCTCCAAA+AGG - chr4_1:78190388-78190407 Msa0574590:intron 35.0%
TTGCAATTGCAGCGAAAATT+CGG - chr4_1:78190460-78190479 Msa0574590:intron 35.0%
ACTGATACAAACTGATCAAC+TGG + chr4_1:78190495-78190514 None:intergenic 35.0%
AGCATTCAAAGCTATATGCA+AGG - chr4_1:78190585-78190604 Msa0574590:intron 35.0%
GATATTTCACCTTTGTCTGT+AGG - chr4_1:78190734-78190753 Msa0574590:intron 35.0%
TTACATTTCCCTACAGACAA+AGG + chr4_1:78190746-78190765 None:intergenic 35.0%
TTTATTTCAAACCTGGTGGT+AGG - chr4_1:78190767-78190786 Msa0574590:intron 35.0%
GCAGCAAGAAATTAATAGTC+TGG - chr4_1:78190789-78190808 Msa0574590:intron 35.0%
GCAAGAAATTAATAGTCTGG+TGG - chr4_1:78190792-78190811 Msa0574590:intron 35.0%
!! AAGGTTCATTGGTTCCTTAA+CGG - chr4_1:78191006-78191025 Msa0574590:intron 35.0%
TCTTACATGGATATCCGTTA+AGG + chr4_1:78191023-78191042 None:intergenic 35.0%
! GTGCTTTTGATACACATGTT+TGG - chr4_1:78191238-78191257 Msa0574590:three_prime_UTR 35.0%
!! TGGTTTTTGGGAGAAAATAG+TGG + chr4_1:78191425-78191444 None:intergenic 35.0%
TTTGTGTCCGACAATAATCA+TGG + chr4_1:78191725-78191744 None:intergenic 35.0%
AAGGATTGCAGAATTTCAGT+TGG - chr4_1:78192063-78192082 Msa0574590:intron 35.0%
!!! TGCCTTTTAGCTTTAATCCA+TGG - chr4_1:78192331-78192350 Msa0574590:three_prime_UTR 35.0%
TGTTTGATTGCTGATGTGTT+AGG - chr4_1:78192444-78192463 Msa0574590:intron 35.0%
TTGTGTTATCACACGAGAAA+AGG - chr4_1:78192747-78192766 Msa0574590:intron 35.0%
!!! GACACTCATTTTGAAACTGA+GGG + chr4_1:78192806-78192825 None:intergenic 35.0%
! GTAATACTTTGGAAGTAGTG+TGG - chr4_1:78192927-78192946 Msa0574590:intron 35.0%
!! GTAGTGTGGAGAGTATTAAT+TGG - chr4_1:78192941-78192960 Msa0574590:intron 35.0%
!! TGTGGAGAGTATTAATTGGA+GGG - chr4_1:78192945-78192964 Msa0574590:intron 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr4_1:78193045-78193064 Msa0574590:intron 35.0%
TATAAAACACATCAAGGAGG+GGG + chr4_1:78193088-78193107 None:intergenic 35.0%
! TTCAGGAACAACATAATGGA+TGG - chr4_1:78193231-78193250 Msa0574590:intron 35.0%
GTATTCTATCAAGTCTTCAG+TGG + chr4_1:78193323-78193342 None:intergenic 35.0%
TTTAAAAGATTAGCACGGCA+TGG + chr4_1:78193388-78193407 None:intergenic 35.0%
! TTTTGTTGGCAGAAATGCAT+TGG - chr4_1:78193426-78193445 Msa0574590:CDS 35.0%
TGAAATATATGGCAAGTGTG+TGG - chr4_1:78193642-78193661 Msa0574590:five_prime_UTR 35.0%
TTGCACCTCAAATGCATTTA+GGG + chr4_1:78193781-78193800 None:intergenic 35.0%
TTTGCACCTCAAATGCATTT+AGG + chr4_1:78193782-78193801 None:intergenic 35.0%
! TAAATGCATTTGAGGTGCAA+AGG - chr4_1:78193781-78193800 Msa0574590:five_prime_UTR 35.0%
TGCAGAGTCCATATTGGAAT+TGG + chr4_1:78189389-78189408 None:intergenic 40.0%
GGTAAAAATGGAGGAGAAAG+GGG + chr4_1:78189426-78189445 None:intergenic 40.0%
GGGTAAAAATGGAGGAGAAA+GGG + chr4_1:78189427-78189446 None:intergenic 40.0%
AGGGTAAAAATGGAGGAGAA+AGG + chr4_1:78189428-78189447 None:intergenic 40.0%
GAAGAGAAGGGTAAAAATGG+AGG + chr4_1:78189435-78189454 None:intergenic 40.0%
GTATTGTTGGGGTTAGAGTT+TGG + chr4_1:78189533-78189552 None:intergenic 40.0%
ATGACTCCATCGTATTGTTG+GGG + chr4_1:78189544-78189563 None:intergenic 40.0%
GATGACTCCATCGTATTGTT+GGG + chr4_1:78189545-78189564 None:intergenic 40.0%
TGATGACTCCATCGTATTGT+TGG + chr4_1:78189546-78189565 None:intergenic 40.0%
ATCTCTCTCAAAACCTCAGA+CGG - chr4_1:78189572-78189591 Msa0574590:three_prime_UTR 40.0%
TTCATGTTCTTGGTGAGAGT+TGG + chr4_1:78189617-78189636 None:intergenic 40.0%
! AGAGAAAGAATTTTCCGTCG+AGG - chr4_1:78189747-78189766 Msa0574590:three_prime_UTR 40.0%
! CGGATCAAGAATAAAAGCGT+TGG - chr4_1:78189863-78189882 Msa0574590:three_prime_UTR 40.0%
!! TGATTTTAATCCGGAGGAAG+AGG - chr4_1:78189916-78189935 Msa0574590:three_prime_UTR 40.0%
GATACAAACTGATCAACTGG+AGG + chr4_1:78190492-78190511 None:intergenic 40.0%
AGCTATATGCAAGGTTGAGA+CGG - chr4_1:78190594-78190613 Msa0574590:intron 40.0%
TGGAACACAATTCCAAGGAT+TGG - chr4_1:78190977-78190996 Msa0574590:intron 40.0%
ATTGGAGATGCAAGGTTCAT+TGG - chr4_1:78190995-78191014 Msa0574590:intron 40.0%
ACCAACATTAAAGTCAGCAG+AGG - chr4_1:78191349-78191368 Msa0574590:intron 40.0%
CCAACATTAAAGTCAGCAGA+GGG - chr4_1:78191350-78191369 Msa0574590:intron 40.0%
AGGGAGCTATTAAAACGAGA+TGG - chr4_1:78191369-78191388 Msa0574590:intron 40.0%
AAAACGAGATGGACCTGTTA+TGG - chr4_1:78191380-78191399 Msa0574590:intron 40.0%
! TTTGCCTTTTCGACCATAAC+AGG + chr4_1:78191396-78191415 None:intergenic 40.0%
GTTATGGTCGAAAAGGCAAA+AGG - chr4_1:78191396-78191415 Msa0574590:intron 40.0%
CCTTACTTCACATTCAACCA+TGG + chr4_1:78192047-78192066 None:intergenic 40.0%
CCATGGTTGAATGTGAAGTA+AGG - chr4_1:78192044-78192063 Msa0574590:intron 40.0%
AGAAAAAGACAGGGAATGCA+AGG - chr4_1:78192107-78192126 Msa0574590:intron 40.0%
GCTGAAACAGAACACAAACA+GGG + chr4_1:78192610-78192629 None:intergenic 40.0%
TGCTGAAACAGAACACAAAC+AGG + chr4_1:78192611-78192630 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACTG+AGG + chr4_1:78192807-78192826 None:intergenic 40.0%
!! GTGTGGAGAGTATTAATTGG+AGG - chr4_1:78192944-78192963 Msa0574590:intron 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr4_1:78193049-78193068 Msa0574590:intron 40.0%
ATAAAACACATCAAGGAGGG+GGG + chr4_1:78193087-78193106 None:intergenic 40.0%
! CTGCTAATTTGGCTCAAAGT+GGG + chr4_1:78193269-78193288 None:intergenic 40.0%
! ACTGCTAATTTGGCTCAAAG+TGG + chr4_1:78193270-78193289 None:intergenic 40.0%
GTCATCAAGGACTGCTAATT+TGG + chr4_1:78193280-78193299 None:intergenic 40.0%
GTATGCCCTAAATGCATTTG+AGG - chr4_1:78193773-78193792 Msa0574590:five_prime_UTR 40.0%
TTGAGGTGCAAAGGTGAAAA+TGG - chr4_1:78193790-78193809 Msa0574590:five_prime_UTR 40.0%
GATACGTGCAGAGTCCATAT+TGG + chr4_1:78189395-78189414 None:intergenic 45.0%
AAAATGGAGGAGAAAGGGGT+GGG + chr4_1:78189422-78189441 None:intergenic 45.0%
AAAAATGGAGGAGAAAGGGG+TGG + chr4_1:78189423-78189442 None:intergenic 45.0%
AGTTTGGACTTCGGAGGATA+GGG + chr4_1:78189517-78189536 None:intergenic 45.0%
TGGGGTTAGAGTTTGGACTT+CGG + chr4_1:78189526-78189545 None:intergenic 45.0%
CTCTAACCCCAACAATACGA+TGG - chr4_1:78189535-78189554 Msa0574590:three_prime_UTR 45.0%
AACGTTGAGGAGAGGAATGA+CGG + chr4_1:78189681-78189700 None:intergenic 45.0%
ATCTTGTTGATGTGGCTGGA+GGG + chr4_1:78189704-78189723 None:intergenic 45.0%
GATGATCTTGTTGATGTGGC+TGG + chr4_1:78189708-78189727 None:intergenic 45.0%
ATCCTTCTTCAGTTCCTCGA+CGG + chr4_1:78189764-78189783 None:intergenic 45.0%
GTTTCTGACTGAGGTTGTTG+TGG - chr4_1:78189838-78189857 Msa0574590:three_prime_UTR 45.0%
TGACTGAGGTTGTTGTGGAT+CGG - chr4_1:78189843-78189862 Msa0574590:three_prime_UTR 45.0%
CACCGACAACATCTATTTGC+AGG + chr4_1:78190221-78190240 None:intergenic 45.0%
GGAAGTGGAACACAATTCCA+AGG - chr4_1:78190972-78190991 Msa0574590:intron 45.0%
TTCCAAGGATTGGAGATGCA+AGG - chr4_1:78190987-78191006 Msa0574590:intron 45.0%
AACCTTGCATCTCCAATCCT+TGG + chr4_1:78190992-78191011 None:intergenic 45.0%
!! TATCTCATGCTTGGTGCTCT+TGG - chr4_1:78191216-78191235 Msa0574590:three_prime_UTR 45.0%
! GGTGTTATGGGCTGAGATTA+GGG + chr4_1:78191332-78191351 None:intergenic 45.0%
!! TGGTGTTATGGGCTGAGATT+AGG + chr4_1:78191333-78191352 None:intergenic 45.0%
!! CCCTCTGCTGACTTTAATGT+TGG + chr4_1:78191353-78191372 None:intergenic 45.0%
TGGACCTGTTATGGTCGAAA+AGG - chr4_1:78191389-78191408 Msa0574590:intron 45.0%
GCATGCAATTCATGAAGCCA+TGG - chr4_1:78192027-78192046 Msa0574590:intron 45.0%
GGACAGCGAATGAATGTAAG+AGG - chr4_1:78192128-78192147 Msa0574590:intron 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr4_1:78193048-78193067 Msa0574590:intron 45.0%
! GGTCATTATGTGTCTGAAGC+TGG + chr4_1:78193302-78193321 None:intergenic 45.0%
!! CATTGGTTCATTCCTTGCGT+GGG - chr4_1:78193443-78193462 Msa0574590:CDS 45.0%
TCAATTTCATGACCCACGCA+AGG + chr4_1:78193458-78193477 None:intergenic 45.0%
!!! TAAAGTTTTTTTTTTTTTTA+GGG - chr4_1:78193552-78193571 Msa0574590:five_prime_UTR 5.0%
GTTTGGACTTCGGAGGATAG+GGG + chr4_1:78189516-78189535 None:intergenic 50.0%
GAGTTTGGACTTCGGAGGAT+AGG + chr4_1:78189518-78189537 None:intergenic 50.0%
GGTTAGAGTTTGGACTTCGG+AGG + chr4_1:78189523-78189542 None:intergenic 50.0%
TTCAAAAACGACGCCGTCTG+AGG + chr4_1:78189588-78189607 None:intergenic 50.0%
!! GTTCTTGGTGAGAGTTGGTG+AGG + chr4_1:78189612-78189631 None:intergenic 50.0%
GATCTTGTTGATGTGGCTGG+AGG + chr4_1:78189705-78189724 None:intergenic 50.0%
TTCCGTCGAGGAACTGAAGA+AGG - chr4_1:78189759-78189778 Msa0574590:three_prime_UTR 50.0%
ATTTCTTGCTGCCTACCACC+AGG + chr4_1:78190781-78190800 None:intergenic 50.0%
ATCGGATGGATCGAAGATGC+TGG - chr4_1:78190948-78190967 Msa0574590:intron 50.0%
ATCGAAGATGCTGGTGGAAG+TGG - chr4_1:78190957-78190976 Msa0574590:intron 50.0%
! GTGTCTGAAGCTGGTCATCA+AGG + chr4_1:78193293-78193312 None:intergenic 50.0%
!! GCATTGGTTCATTCCTTGCG+TGG - chr4_1:78193442-78193461 Msa0574590:CDS 50.0%
! GAGAGGAATGACGGAGACGT+GGG + chr4_1:78189672-78189691 None:intergenic 55.0%
CTACACTCTGCCTCTTCCTC+CGG + chr4_1:78189929-78189948 None:intergenic 55.0%
ACTGTGTGCCTTCAAACCCC+CGG - chr4_1:78189951-78189970 Msa0574590:three_prime_UTR 55.0%
GGATGGATCGAAGATGCTGG+TGG - chr4_1:78190951-78190970 Msa0574590:intron 55.0%
TAGCACGGCATGGTTCGTAC+TGG + chr4_1:78193378-78193397 None:intergenic 55.0%
! GGAGAGGAATGACGGAGACG+TGG + chr4_1:78189673-78189692 None:intergenic 60.0%
CTGGAGGGAACGTTGAGGAG+AGG + chr4_1:78189689-78189708 None:intergenic 60.0%
TGTGGCTGGAGGGAACGTTG+AGG + chr4_1:78189694-78189713 None:intergenic 60.0%
CGGAGGAAGAGGCAGAGTGT+AGG - chr4_1:78189927-78189946 Msa0574590:three_prime_UTR 60.0%
TCACCTTGATCCACCGCTCC+GGG + chr4_1:78189972-78189991 None:intergenic 60.0%
GTCACCTTGATCCACCGCTC+CGG + chr4_1:78189973-78189992 None:intergenic 60.0%
GTGCCTTCAAACCCCCGGAG+CGG - chr4_1:78189956-78189975 Msa0574590:three_prime_UTR 65.0%
ACCTTGATCCACCGCTCCGG+GGG + chr4_1:78189970-78189989 None:intergenic 65.0%
CACCTTGATCCACCGCTCCG+GGG + chr4_1:78189971-78189990 None:intergenic 65.0%
CCACCGCTCCGGGGGTTTGA+AGG + chr4_1:78189962-78189981 None:intergenic 70.0%
CCTTCAAACCCCCGGAGCGG+TGG - chr4_1:78189959-78189978 Msa0574590:three_prime_UTR 70.0%
ACCCCCGGAGCGGTGGATCA+AGG - chr4_1:78189966-78189985 Msa0574590:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 78189319 78193974 78189319 ID=Msa0574590;Name=Msa0574590
chr4_1 mRNA 78189319 78193974 78189319 ID=Msa0574590-mRNA-1;Parent=Msa0574590;Name=Msa0574590-mRNA-1;_AED=0.17;_eAED=0.17;_QI=463|1|1|1|0.25|0.2|5|1046|71
chr4_1 exon 78189319 78190057 78189319 ID=Msa0574590-mRNA-1:exon:27746;Parent=Msa0574590-mRNA-1
chr4_1 exon 78191151 78191274 78191151 ID=Msa0574590-mRNA-1:exon:27745;Parent=Msa0574590-mRNA-1
chr4_1 exon 78192257 78192391 78192257 ID=Msa0574590-mRNA-1:exon:27744;Parent=Msa0574590-mRNA-1
chr4_1 exon 78192480 78192537 78192480 ID=Msa0574590-mRNA-1:exon:27743;Parent=Msa0574590-mRNA-1
chr4_1 exon 78193306 78193974 78193306 ID=Msa0574590-mRNA-1:exon:27742;Parent=Msa0574590-mRNA-1
chr4_1 five_prime_UTR 78193512 78193974 78193512 ID=Msa0574590-mRNA-1:five_prime_utr;Parent=Msa0574590-mRNA-1
chr4_1 CDS 78193306 78193511 78193306 ID=Msa0574590-mRNA-1:cds;Parent=Msa0574590-mRNA-1
chr4_1 CDS 78192528 78192537 78192528 ID=Msa0574590-mRNA-1:cds;Parent=Msa0574590-mRNA-1
chr4_1 three_prime_UTR 78192480 78192527 78192480 ID=Msa0574590-mRNA-1:three_prime_utr;Parent=Msa0574590-mRNA-1
chr4_1 three_prime_UTR 78192257 78192391 78192257 ID=Msa0574590-mRNA-1:three_prime_utr;Parent=Msa0574590-mRNA-1
chr4_1 three_prime_UTR 78191151 78191274 78191151 ID=Msa0574590-mRNA-1:three_prime_utr;Parent=Msa0574590-mRNA-1
chr4_1 three_prime_UTR 78189319 78190057 78189319 ID=Msa0574590-mRNA-1:three_prime_utr;Parent=Msa0574590-mRNA-1
Gene Sequence

>Msa0574590

ATGTTGAAAGAGTTTCTTCTTTCAGTGATTTACTTGAATATGGAGAGTCTTTTTGAGTTTCTGACTGAGGTTGTTGTGGATCGGATCAAGAATAAAAGCGTTGGGTATGTGAGAAAGTATTTCGGTGTTGAAAATGATTTTAATCCGGAGGAAGAGGCAGAGTGTAGGAACTGTGTGCCTTCAAACCCCCGGAGCGGTGGATCAAGGGAAATGTAA
Protein sequence

>Msa0574590

MLKEFLLSVIYLNMESLFEFLTEVVVDRIKNKSVGYVRKYFGVENDFNPEEEAECRNCVPSNPRSGGSREM*