Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006070 | Msa0852420 | 0.829654 | 4.562301e-55 | -8.615850e-47 |
| Msa0802010 | Msa0852420 | 0.800002 | 1.767778e-48 | -8.615850e-47 |
| Msa0574590 | Msa0852420 | 0.835420 | 1.695605e-56 | -8.615850e-47 |
| Msa0852410 | Msa0852420 | 0.914887 | 1.130721e-84 | -8.615850e-47 |
| Msa0852420 | Msa0994030 | 0.814360 | 1.603210e-51 | -8.615850e-47 |
| Msa0852420 | Msa1228590 | 0.801170 | 1.021702e-48 | -8.615850e-47 |
| Msa0852420 | Msa1374470 | 0.812189 | 4.804221e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCAACTCTTCCTCTGTTAC+AGG | 0.223910 | 5_4:+43478232 | Msa0852420:CDS |
| AAAGACGTCCTACGGCGAAT+GGG | 0.388959 | 5_4:-43477796 | None:intergenic |
| AAAACTTGCAAATGAAGAAC+TGG | 0.423959 | 5_4:-43478186 | None:intergenic |
| TAAAGACGTCCTACGGCGAA+TGG | 0.471070 | 5_4:-43477797 | None:intergenic |
| AACAATTATGAATCGCCGAT+TGG | 0.486980 | 5_4:+43477757 | None:intergenic |
| ACACATCATTGTCCACCAAT+CGG | 0.532246 | 5_4:-43477772 | None:intergenic |
| TTCCTCTGTTACAGGCTCAC+GGG | 0.552148 | 5_4:+43478240 | Msa0852420:CDS |
| GCCTGTAACAGAGGAAGAGT+TGG | 0.557877 | 5_4:-43478233 | None:intergenic |
| CTCCCGTGAGCCTGTAACAG+AGG | 0.565108 | 5_4:-43478242 | None:intergenic |
| CTTCCTCTGTTACAGGCTCA+CGG | 0.569215 | 5_4:+43478239 | Msa0852420:CDS |
| AATTATGAATCGCCGATTGG+TGG | 0.569270 | 5_4:+43477760 | None:intergenic |
| AAGACGTCCTACGGCGAATG+GGG | 0.570942 | 5_4:-43477795 | None:intergenic |
| AGAACCTGAGTTATCAGCAC+AGG | 0.592586 | 5_4:-43478161 | None:intergenic |
| TAATCCTGTGCTGATAACTC+AGG | 0.594796 | 5_4:+43478157 | Msa0852420:CDS |
| AGAAACATAAAGACGTCCTA+CGG | 0.621863 | 5_4:-43477804 | None:intergenic |
| GATGTGTCCCCATTCGCCGT+AGG | 0.641646 | 5_4:+43477788 | Msa0852420:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATCATGCTTTAATTTAATT+TGG | + | chr5_4:43477995-43478014 | Msa0852420:intron | 15.0% |
| !!! | TTTCTAGTAATAATGTAATG+CGG | + | chr5_4:43477822-43477841 | Msa0852420:intron | 20.0% |
| !! | AATTAAAGCATGATAGAAAA+AGG | - | chr5_4:43477990-43478009 | None:intergenic | 20.0% |
| TGTTATATATAGCTTACCAC+AGG | + | chr5_4:43477953-43477972 | Msa0852420:intron | 30.0% | |
| !!! | TCTTCCACTTTTGTTTTCTA+TGG | + | chr5_4:43478109-43478128 | Msa0852420:intron | 30.0% |
| AGAACCATAGAAAACAAAAG+TGG | - | chr5_4:43478116-43478135 | None:intergenic | 30.0% | |
| AAAACTTGCAAATGAAGAAC+TGG | - | chr5_4:43478189-43478208 | None:intergenic | 30.0% | |
| ! | GTTCTTCATTTGCAAGTTTT+TGG | + | chr5_4:43478189-43478208 | Msa0852420:CDS | 30.0% |
| !!! | TTGCAAGTTTTTGGAATCTT+AGG | + | chr5_4:43478198-43478217 | Msa0852420:CDS | 30.0% |
| AGAAACATAAAGACGTCCTA+CGG | - | chr5_4:43477807-43477826 | None:intergenic | 35.0% | |
| AAAGTAGCTTGACTGTTGAA+TGG | + | chr5_4:43477850-43477869 | Msa0852420:intron | 35.0% | |
| AGTCAGATTCTCAAGTAAGT+TGG | - | chr5_4:43477934-43477953 | None:intergenic | 35.0% | |
| ACCATATTCTAGTATGAGTC+TGG | + | chr5_4:43478073-43478092 | Msa0852420:intron | 35.0% | |
| ACCAGACTCATACTAGAATA+TGG | - | chr5_4:43478077-43478096 | None:intergenic | 35.0% | |
| ACACATCATTGTCCACCAAT+CGG | - | chr5_4:43477775-43477794 | None:intergenic | 40.0% | |
| GCTTACCACAGGATATATTC+AGG | + | chr5_4:43477964-43477983 | Msa0852420:intron | 40.0% | |
| AAAGGCCTGAATATATCCTG+TGG | - | chr5_4:43477972-43477991 | None:intergenic | 40.0% | |
| ! | TAATCCTGTGCTGATAACTC+AGG | + | chr5_4:43478157-43478176 | Msa0852420:CDS | 40.0% |
| AGAACCTGAGTTATCAGCAC+AGG | - | chr5_4:43478164-43478183 | None:intergenic | 45.0% | |
| AAAGACGTCCTACGGCGAAT+GGG | - | chr5_4:43477799-43477818 | None:intergenic | 50.0% | |
| TAAAGACGTCCTACGGCGAA+TGG | - | chr5_4:43477800-43477819 | None:intergenic | 50.0% | |
| GCCAACTCTTCCTCTGTTAC+AGG | + | chr5_4:43478232-43478251 | Msa0852420:CDS | 50.0% | |
| GCCTGTAACAGAGGAAGAGT+TGG | - | chr5_4:43478236-43478255 | None:intergenic | 50.0% | |
| CTTCCTCTGTTACAGGCTCA+CGG | + | chr5_4:43478239-43478258 | Msa0852420:CDS | 50.0% | |
| TTCCTCTGTTACAGGCTCAC+GGG | + | chr5_4:43478240-43478259 | Msa0852420:CDS | 50.0% | |
| AAGACGTCCTACGGCGAATG+GGG | - | chr5_4:43477798-43477817 | None:intergenic | 55.0% | |
| GATGTGTCCCCATTCGCCGT+AGG | + | chr5_4:43477788-43477807 | Msa0852420:CDS | 60.0% | |
| CTCCCGTGAGCCTGTAACAG+AGG | - | chr5_4:43478245-43478264 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_4 | gene | 43477764 | 43478268 | 43477764 | ID=Msa0852420;Name=Msa0852420 |
| chr5_4 | mRNA | 43477764 | 43478268 | 43477764 | ID=Msa0852420-mRNA-1;Parent=Msa0852420;Name=Msa0852420-mRNA-1;_AED=0.47;_eAED=1.00;_QI=0|0|0|1|1|1|2|0|64 |
| chr5_4 | exon | 43477764 | 43477835 | 43477764 | ID=Msa0852420-mRNA-1:exon:13744;Parent=Msa0852420-mRNA-1 |
| chr5_4 | exon | 43478146 | 43478268 | 43478146 | ID=Msa0852420-mRNA-1:exon:13745;Parent=Msa0852420-mRNA-1 |
| chr5_4 | CDS | 43477764 | 43477835 | 43477764 | ID=Msa0852420-mRNA-1:cds;Parent=Msa0852420-mRNA-1 |
| chr5_4 | CDS | 43478146 | 43478268 | 43478146 | ID=Msa0852420-mRNA-1:cds;Parent=Msa0852420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
