AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0574720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574720 MtrunA17_Chr8g0391461 95.000 320 16 0 1 320 1 320 0.0 624
Msa0574720 MtrunA17_Chr8g0376931 30.935 278 166 5 26 278 279 555 3.89e-39 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0574720 AT2G01620.1 40.956 293 130 7 26 307 17 277 7.92e-69 216
Msa0574720 AT3G26000.1 33.582 268 173 3 12 278 187 450 4.65e-47 164
Msa0574720 AT3G27290.1 29.644 253 166 6 26 272 128 374 1.32e-29 116

Find 84 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTATTGCAGTCCACTTT+TGG 0.183685 4_1:-78407981 None:intergenic
TCCTCATGAAGCCATGTTTC+TGG 0.211699 4_1:+78407013 Msa0574720:CDS
AAACAAGAAGATTATTGATT+TGG 0.242718 4_1:+78407914 Msa0574720:CDS
GATTTCGGTCATGCTTAAAA+AGG 0.265888 4_1:+78408445 Msa0574720:three_prime_UTR
CACAGCCGTTAGAACTCCTT+CGG 0.318340 4_1:-78407703 None:intergenic
CGCAACTTCTTCAAGTTCTT+CGG 0.331082 4_1:-78408081 None:intergenic
CATACACTGATGTTCTATAA+TGG 0.342115 4_1:+78408258 Msa0574720:CDS
GAATCAAATCAAAACAAATA+TGG 0.347356 4_1:+78406923 Msa0574720:exon
TAACTGAGCCAACATGATTT+CGG 0.370607 4_1:+78408430 Msa0574720:three_prime_UTR
TGGTTAAGCAGTTGGCTCTT+TGG 0.371025 4_1:-78407737 None:intergenic
TTGAAGCCTACCATTTGCTT+TGG 0.382156 4_1:-78407195 None:intergenic
GCCCAAAAGTGGACTGCAAT+AGG 0.399387 4_1:+78407979 Msa0574720:CDS
TGTGTTGGCTCGTCTTCTAA+TGG 0.400719 4_1:-78407837 None:intergenic
TTAACCACCTTGAGTTTAAA+CGG 0.409604 4_1:+78407753 Msa0574720:CDS
TTAATATCATATGATATGAT+TGG 0.419761 4_1:+78406860 Msa0574720:five_prime_UTR
CTGATTTAAATGCCAAGTAT+TGG 0.431362 4_1:-78408534 None:intergenic
ATTGTAAGCAGCACACAAAT+TGG 0.441410 4_1:+78408171 Msa0574720:CDS
ACAGCCGTTAGAACTCCTTC+GGG 0.460182 4_1:-78407702 None:intergenic
CTTCATATACCAGCATGCAC+TGG 0.461330 4_1:+78407672 Msa0574720:CDS
ATAAAAGAAGGAGGTAAGCT+TGG 0.466353 4_1:-78406824 None:intergenic
TTTGTGTGCTGCTTACAATA+TGG 0.466361 4_1:-78408167 None:intergenic
CCACGTTTATGATAGTAGAT+AGG 0.466789 4_1:-78407864 None:intergenic
GATATATGACTGCCCAAAAG+TGG 0.473918 4_1:+78407968 Msa0574720:CDS
ACATTACCTCTTCAATAAAG+AGG 0.477963 4_1:-78407283 None:intergenic
ACAAATATATTTATAAAAGA+AGG 0.480069 4_1:-78406836 None:intergenic
TGCATCCGTATCTATGTGGC+TGG 0.483813 4_1:-78406784 None:intergenic
CATTCTCACCTCAAAGCACT+TGG 0.484732 4_1:-78407946 None:intergenic
TACTGACCATGGACTACAAA+GGG 0.495839 4_1:+78407247 Msa0574720:CDS
AAGATCACATCCAAAGCAAA+TGG 0.499356 4_1:+78407185 Msa0574720:CDS
CAACAACCCTTTGTAGTCCA+TGG 0.509847 4_1:-78407253 None:intergenic
ACAAAACCTCTTTATTGAAG+AGG 0.511417 4_1:+78407277 Msa0574720:CDS
TGCATCCCCTACATTGAGCT+TGG 0.519283 4_1:-78408013 None:intergenic
ATTTCTTGTGCCTTGAATGT+TGG 0.519349 4_1:+78408111 Msa0574720:CDS
CTGAGAGTCTTGAATTGAGA+GGG 0.521633 4_1:-78407148 None:intergenic
TTGGCTCGTCTTCTAATGGA+AGG 0.527622 4_1:-78407833 None:intergenic
AATCGCGCCAAGCTCAATGT+AGG 0.530209 4_1:+78408006 Msa0574720:CDS
ATCGCGCCAAGCTCAATGTA+GGG 0.531342 4_1:+78408007 Msa0574720:CDS
GTAAATGCCGTTTAAACTCA+AGG 0.533829 4_1:-78407760 None:intergenic
ACTCAAGGTGGTTAAGCAGT+TGG 0.536638 4_1:-78407745 None:intergenic
GAAAACTGTGGTGGATGTGT+TGG 0.545086 4_1:+78408056 Msa0574720:CDS
TTCATATACCAGCATGCACT+GGG 0.550386 4_1:+78407673 Msa0574720:CDS
AGCATCGAGAGACCAATACT+TGG 0.551005 4_1:+78408522 Msa0574720:three_prime_UTR
GGGGTTATCCCAGTGCATGC+TGG 0.558348 4_1:-78407681 None:intergenic
TTTGTTACAGAAATTGCACT+TGG 0.560253 4_1:-78408144 None:intergenic
TGTTCAACAATAATTTCTGT+AGG 0.561774 4_1:-78408363 None:intergenic
GTTTCCCAGCCACATAGATA+CGG 0.571916 4_1:+78406779 Msa0574720:five_prime_UTR
GAATTGTCCCAAGTGCTTTG+AGG 0.572633 4_1:+78407938 Msa0574720:CDS
TCAACAATGATGTATTGCTA+TGG 0.574377 4_1:+78407105 Msa0574720:CDS
TGCATCCACATTACTGACCA+TGG 0.576552 4_1:+78407236 Msa0574720:CDS
GAAGCAGTAACCAACATTCA+AGG 0.577227 4_1:-78408121 None:intergenic
AGTTGAGGAAGCAGAACAAG+AGG 0.586847 4_1:+78406986 Msa0574720:CDS
TGAAACGAGCACCACAGTTG+AGG 0.592271 4_1:+78406971 Msa0574720:CDS
AATATATTTATAAAAGAAGG+AGG 0.594808 4_1:-78406833 None:intergenic
CAGCCGTTAGAACTCCTTCG+GGG 0.597481 4_1:-78407701 None:intergenic
TTGTTCTGCTTCCTCAACTG+TGG 0.601003 4_1:-78406982 None:intergenic
AAGCATGACCGAAATCATGT+TGG 0.602708 4_1:-78408438 None:intergenic
TCACATCCAAAGCAAATGGT+AGG 0.604181 4_1:+78407189 Msa0574720:CDS
AGCTTACCTTCGAGTGTACG+AGG 0.604187 4_1:+78407040 Msa0574720:CDS
AAAAGTGGACTGCAATAGGA+AGG 0.608381 4_1:+78407983 Msa0574720:CDS
GTAAGCAGCACACAAATTGG+TGG 0.609215 4_1:+78408174 Msa0574720:CDS
GTAAGCTATCACCAGAAACA+TGG 0.609754 4_1:-78407024 None:intergenic
AGAAACATGGCTTCATGAGG+AGG 0.615086 4_1:-78407011 None:intergenic
ATTCTCACCTCAAAGCACTT+GGG 0.615893 4_1:-78407945 None:intergenic
GTTGAGGAAGCAGAACAAGA+GGG 0.631563 4_1:+78406987 Msa0574720:CDS
ACCAGAAACATGGCTTCATG+AGG 0.632114 4_1:-78407014 None:intergenic
TGAGAGTCTTGAATTGAGAG+GGG 0.638788 4_1:-78407147 None:intergenic
TGCAGTAACTCAAAATGACT+AGG 0.639487 4_1:-78408319 None:intergenic
TTAAAACCTCGTACACTCGA+AGG 0.640853 4_1:-78407046 None:intergenic
GTAGTCCATGGTCAGTAATG+TGG 0.645529 4_1:-78407241 None:intergenic
TCTGAGAGTCTTGAATTGAG+AGG 0.649051 4_1:-78407149 None:intergenic
TGATGTGCAAACACAAGACA+TGG 0.656078 4_1:-78407069 None:intergenic
GTGTGTGACGAAAATTCTCA+CGG 0.664023 4_1:+78408230 Msa0574720:CDS
TTACTGACCATGGACTACAA+AGG 0.669179 4_1:+78407246 Msa0574720:CDS
TCGCGCCAAGCTCAATGTAG+GGG 0.671237 4_1:+78408008 Msa0574720:CDS
CCAAGGTGTGAAAACTGTGG+TGG 0.671238 4_1:+78408047 Msa0574720:CDS
AGCCGTTAGAACTCCTTCGG+GGG 0.675366 4_1:-78407700 None:intergenic
TTGTTACAGAAATTGCACTT+GGG 0.679098 4_1:-78408143 None:intergenic
GCATCCGTATCTATGTGGCT+GGG 0.680249 4_1:-78406783 None:intergenic
TAGTAGATAGGCTGTTGTGT+TGG 0.686387 4_1:-78407852 None:intergenic
CCTATCTACTATCATAAACG+TGG 0.693271 4_1:+78407864 Msa0574720:CDS
GAACTTGAAGAAGTTGCGTG+CGG 0.702138 4_1:+78408086 Msa0574720:CDS
AATGCCGTTTAAACTCAAGG+TGG 0.706837 4_1:-78407757 None:intergenic
ATGTTGCATCCGTATCTATG+TGG 0.729142 4_1:-78406788 None:intergenic
ATTCCAAGGTGTGAAAACTG+TGG 0.793812 4_1:+78408044 Msa0574720:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAATATATTTATAAAAGA+AGG - chr4_1:78406839-78406858 None:intergenic 10.0%
!!! TAAAATTTTATAAAAACCAT+AGG - chr4_1:78407572-78407591 None:intergenic 10.0%
!! ACAAATATATTTATAAAAGA+AGG - chr4_1:78406839-78406858 None:intergenic 10.0%
!!! TAAAATTTTATAAAAACCAT+AGG - chr4_1:78407572-78407591 None:intergenic 10.0%
!! AATATATTTATAAAAGAAGG+AGG - chr4_1:78406836-78406855 None:intergenic 15.0%
!! TTAATATCATATGATATGAT+TGG + chr4_1:78406860-78406879 Msa0574720:five_prime_UTR 15.0%
!!! AATACAAAATTGAGTTTTTT+AGG + chr4_1:78407606-78407625 Msa0574720:intron 15.0%
!! AATATATTTATAAAAGAAGG+AGG - chr4_1:78406836-78406855 None:intergenic 15.0%
!! TTAATATCATATGATATGAT+TGG + chr4_1:78406860-78406879 Msa0574720:five_prime_UTR 15.0%
!!! AATACAAAATTGAGTTTTTT+AGG + chr4_1:78407606-78407625 Msa0574720:intron 15.0%
!! GAATCAAATCAAAACAAATA+TGG + chr4_1:78406923-78406942 Msa0574720:exon 20.0%
!!! AAACAAGAAGATTATTGATT+TGG + chr4_1:78407914-78407933 Msa0574720:CDS 20.0%
!! GAATCAAATCAAAACAAATA+TGG + chr4_1:78406923-78406942 Msa0574720:exon 20.0%
!!! AAACAAGAAGATTATTGATT+TGG + chr4_1:78407914-78407933 Msa0574720:CDS 20.0%
!! ATTTTGCTACTATGATTCAA+TGG + chr4_1:78407322-78407341 Msa0574720:intron 25.0%
! TGTTCAACAATAATTTCTGT+AGG - chr4_1:78408366-78408385 None:intergenic 25.0%
!! ATTTTGCTACTATGATTCAA+TGG + chr4_1:78407322-78407341 Msa0574720:intron 25.0%
! TGTTCAACAATAATTTCTGT+AGG - chr4_1:78408366-78408385 None:intergenic 25.0%
TCAACAATGATGTATTGCTA+TGG + chr4_1:78407105-78407124 Msa0574720:CDS 30.0%
ACAAAACCTCTTTATTGAAG+AGG + chr4_1:78407277-78407296 Msa0574720:CDS 30.0%
ACATTACCTCTTCAATAAAG+AGG - chr4_1:78407286-78407305 None:intergenic 30.0%
!!! GTTTTAGCTGCTTAACAATT+AGG + chr4_1:78407394-78407413 Msa0574720:intron 30.0%
!!! ACTATTCCTAGGTTTTGAAT+TGG + chr4_1:78407494-78407513 Msa0574720:intron 30.0%
TTAACCACCTTGAGTTTAAA+CGG + chr4_1:78407753-78407772 Msa0574720:CDS 30.0%
! GATGCATATTTTGTATTCCA+AGG + chr4_1:78408030-78408049 Msa0574720:CDS 30.0%
TTGTTACAGAAATTGCACTT+GGG - chr4_1:78408146-78408165 None:intergenic 30.0%
TTTGTTACAGAAATTGCACT+TGG - chr4_1:78408147-78408166 None:intergenic 30.0%
CATACACTGATGTTCTATAA+TGG + chr4_1:78408258-78408277 Msa0574720:CDS 30.0%
TCAACAATGATGTATTGCTA+TGG + chr4_1:78407105-78407124 Msa0574720:CDS 30.0%
ACAAAACCTCTTTATTGAAG+AGG + chr4_1:78407277-78407296 Msa0574720:CDS 30.0%
ACATTACCTCTTCAATAAAG+AGG - chr4_1:78407286-78407305 None:intergenic 30.0%
!!! GTTTTAGCTGCTTAACAATT+AGG + chr4_1:78407394-78407413 Msa0574720:intron 30.0%
!!! ACTATTCCTAGGTTTTGAAT+TGG + chr4_1:78407494-78407513 Msa0574720:intron 30.0%
TTAACCACCTTGAGTTTAAA+CGG + chr4_1:78407753-78407772 Msa0574720:CDS 30.0%
! GATGCATATTTTGTATTCCA+AGG + chr4_1:78408030-78408049 Msa0574720:CDS 30.0%
TTGTTACAGAAATTGCACTT+GGG - chr4_1:78408146-78408165 None:intergenic 30.0%
TTTGTTACAGAAATTGCACT+TGG - chr4_1:78408147-78408166 None:intergenic 30.0%
CATACACTGATGTTCTATAA+TGG + chr4_1:78408258-78408277 Msa0574720:CDS 30.0%
ATAAAAGAAGGAGGTAAGCT+TGG - chr4_1:78406827-78406846 None:intergenic 35.0%
AAGATCACATCCAAAGCAAA+TGG + chr4_1:78407185-78407204 Msa0574720:CDS 35.0%
ACAATTAGGATAGTGAGCAA+AGG + chr4_1:78407408-78407427 Msa0574720:intron 35.0%
CAATTAGGATAGTGAGCAAA+GGG + chr4_1:78407409-78407428 Msa0574720:intron 35.0%
TTCAATCACAGTCTGATTTC+TGG + chr4_1:78407632-78407651 Msa0574720:intron 35.0%
! GTAAATGCCGTTTAAACTCA+AGG - chr4_1:78407763-78407782 None:intergenic 35.0%
!!! AAGGTTTTTTCTGAGGTTTG+AGG - chr4_1:78407817-78407836 None:intergenic 35.0%
!!! CTAATGGAAGGTTTTTTCTG+AGG - chr4_1:78407824-78407843 None:intergenic 35.0%
CCACGTTTATGATAGTAGAT+AGG - chr4_1:78407867-78407886 None:intergenic 35.0%
CCTATCTACTATCATAAACG+TGG + chr4_1:78407864-78407883 Msa0574720:CDS 35.0%
ATTTCTTGTGCCTTGAATGT+TGG + chr4_1:78408111-78408130 Msa0574720:CDS 35.0%
!! TTTGTGTGCTGCTTACAATA+TGG - chr4_1:78408170-78408189 None:intergenic 35.0%
ATTGTAAGCAGCACACAAAT+TGG + chr4_1:78408171-78408190 Msa0574720:CDS 35.0%
TGCAGTAACTCAAAATGACT+AGG - chr4_1:78408322-78408341 None:intergenic 35.0%
TAACTGAGCCAACATGATTT+CGG + chr4_1:78408430-78408449 Msa0574720:three_prime_UTR 35.0%
GATTTCGGTCATGCTTAAAA+AGG + chr4_1:78408445-78408464 Msa0574720:three_prime_UTR 35.0%
ATGCTTAAAAAGGCTGAGTT+TGG + chr4_1:78408455-78408474 Msa0574720:three_prime_UTR 35.0%
ATAAAAGAAGGAGGTAAGCT+TGG - chr4_1:78406827-78406846 None:intergenic 35.0%
AAGATCACATCCAAAGCAAA+TGG + chr4_1:78407185-78407204 Msa0574720:CDS 35.0%
ACAATTAGGATAGTGAGCAA+AGG + chr4_1:78407408-78407427 Msa0574720:intron 35.0%
CAATTAGGATAGTGAGCAAA+GGG + chr4_1:78407409-78407428 Msa0574720:intron 35.0%
TTCAATCACAGTCTGATTTC+TGG + chr4_1:78407632-78407651 Msa0574720:intron 35.0%
! GTAAATGCCGTTTAAACTCA+AGG - chr4_1:78407763-78407782 None:intergenic 35.0%
!!! AAGGTTTTTTCTGAGGTTTG+AGG - chr4_1:78407817-78407836 None:intergenic 35.0%
!!! CTAATGGAAGGTTTTTTCTG+AGG - chr4_1:78407824-78407843 None:intergenic 35.0%
CCACGTTTATGATAGTAGAT+AGG - chr4_1:78407867-78407886 None:intergenic 35.0%
CCTATCTACTATCATAAACG+TGG + chr4_1:78407864-78407883 Msa0574720:CDS 35.0%
ATTTCTTGTGCCTTGAATGT+TGG + chr4_1:78408111-78408130 Msa0574720:CDS 35.0%
!! TTTGTGTGCTGCTTACAATA+TGG - chr4_1:78408170-78408189 None:intergenic 35.0%
ATTGTAAGCAGCACACAAAT+TGG + chr4_1:78408171-78408190 Msa0574720:CDS 35.0%
TGCAGTAACTCAAAATGACT+AGG - chr4_1:78408322-78408341 None:intergenic 35.0%
TAACTGAGCCAACATGATTT+CGG + chr4_1:78408430-78408449 Msa0574720:three_prime_UTR 35.0%
GATTTCGGTCATGCTTAAAA+AGG + chr4_1:78408445-78408464 Msa0574720:three_prime_UTR 35.0%
ATGCTTAAAAAGGCTGAGTT+TGG + chr4_1:78408455-78408474 Msa0574720:three_prime_UTR 35.0%
ATGTTGCATCCGTATCTATG+TGG - chr4_1:78406791-78406810 None:intergenic 40.0%
GTAAGCTATCACCAGAAACA+TGG - chr4_1:78407027-78407046 None:intergenic 40.0%
TTAAAACCTCGTACACTCGA+AGG - chr4_1:78407049-78407068 None:intergenic 40.0%
TGATGTGCAAACACAAGACA+TGG - chr4_1:78407072-78407091 None:intergenic 40.0%
TGAGAGTCTTGAATTGAGAG+GGG - chr4_1:78407150-78407169 None:intergenic 40.0%
CTGAGAGTCTTGAATTGAGA+GGG - chr4_1:78407151-78407170 None:intergenic 40.0%
TCTGAGAGTCTTGAATTGAG+AGG - chr4_1:78407152-78407171 None:intergenic 40.0%
TCACATCCAAAGCAAATGGT+AGG + chr4_1:78407189-78407208 Msa0574720:CDS 40.0%
TTGAAGCCTACCATTTGCTT+TGG - chr4_1:78407198-78407217 None:intergenic 40.0%
! TTACTGACCATGGACTACAA+AGG + chr4_1:78407246-78407265 Msa0574720:CDS 40.0%
! TACTGACCATGGACTACAAA+GGG + chr4_1:78407247-78407266 Msa0574720:CDS 40.0%
AGGTAGCCAATTCAAAACCT+AGG - chr4_1:78407503-78407522 None:intergenic 40.0%
CAATCCTCAACACAATGGTA+AGG - chr4_1:78407545-78407564 None:intergenic 40.0%
GTGAACAATCCTCAACACAA+TGG - chr4_1:78407550-78407569 None:intergenic 40.0%
TGTTGAGGATTGTTCACCTA+TGG + chr4_1:78407553-78407572 Msa0574720:intron 40.0%
TTCATATACCAGCATGCACT+GGG + chr4_1:78407673-78407692 Msa0574720:CDS 40.0%
! AATGCCGTTTAAACTCAAGG+TGG - chr4_1:78407760-78407779 None:intergenic 40.0%
! CGTCAAGATGATCGTTTTGT+AGG - chr4_1:78407788-78407807 None:intergenic 40.0%
TAGTAGATAGGCTGTTGTGT+TGG - chr4_1:78407855-78407874 None:intergenic 40.0%
! ATTCTCACCTCAAAGCACTT+GGG - chr4_1:78407948-78407967 None:intergenic 40.0%
GATATATGACTGCCCAAAAG+TGG + chr4_1:78407968-78407987 Msa0574720:CDS 40.0%
!! TCCTATTGCAGTCCACTTTT+GGG - chr4_1:78407983-78408002 None:intergenic 40.0%
! TTCCTATTGCAGTCCACTTT+TGG - chr4_1:78407984-78408003 None:intergenic 40.0%
!! AAAAGTGGACTGCAATAGGA+AGG + chr4_1:78407983-78408002 Msa0574720:CDS 40.0%
ATTCCAAGGTGTGAAAACTG+TGG + chr4_1:78408044-78408063 Msa0574720:CDS 40.0%
! CGCAACTTCTTCAAGTTCTT+CGG - chr4_1:78408084-78408103 None:intergenic 40.0%
GAAGCAGTAACCAACATTCA+AGG - chr4_1:78408124-78408143 None:intergenic 40.0%
GTGTGTGACGAAAATTCTCA+CGG + chr4_1:78408230-78408249 Msa0574720:CDS 40.0%
AAGCATGACCGAAATCATGT+TGG - chr4_1:78408441-78408460 None:intergenic 40.0%
ATGTTGCATCCGTATCTATG+TGG - chr4_1:78406791-78406810 None:intergenic 40.0%
GTAAGCTATCACCAGAAACA+TGG - chr4_1:78407027-78407046 None:intergenic 40.0%
TTAAAACCTCGTACACTCGA+AGG - chr4_1:78407049-78407068 None:intergenic 40.0%
TGATGTGCAAACACAAGACA+TGG - chr4_1:78407072-78407091 None:intergenic 40.0%
TGAGAGTCTTGAATTGAGAG+GGG - chr4_1:78407150-78407169 None:intergenic 40.0%
CTGAGAGTCTTGAATTGAGA+GGG - chr4_1:78407151-78407170 None:intergenic 40.0%
TCTGAGAGTCTTGAATTGAG+AGG - chr4_1:78407152-78407171 None:intergenic 40.0%
TCACATCCAAAGCAAATGGT+AGG + chr4_1:78407189-78407208 Msa0574720:CDS 40.0%
TTGAAGCCTACCATTTGCTT+TGG - chr4_1:78407198-78407217 None:intergenic 40.0%
! TTACTGACCATGGACTACAA+AGG + chr4_1:78407246-78407265 Msa0574720:CDS 40.0%
! TACTGACCATGGACTACAAA+GGG + chr4_1:78407247-78407266 Msa0574720:CDS 40.0%
AGGTAGCCAATTCAAAACCT+AGG - chr4_1:78407503-78407522 None:intergenic 40.0%
CAATCCTCAACACAATGGTA+AGG - chr4_1:78407545-78407564 None:intergenic 40.0%
GTGAACAATCCTCAACACAA+TGG - chr4_1:78407550-78407569 None:intergenic 40.0%
TGTTGAGGATTGTTCACCTA+TGG + chr4_1:78407553-78407572 Msa0574720:intron 40.0%
TTCATATACCAGCATGCACT+GGG + chr4_1:78407673-78407692 Msa0574720:CDS 40.0%
! AATGCCGTTTAAACTCAAGG+TGG - chr4_1:78407760-78407779 None:intergenic 40.0%
! CGTCAAGATGATCGTTTTGT+AGG - chr4_1:78407788-78407807 None:intergenic 40.0%
TAGTAGATAGGCTGTTGTGT+TGG - chr4_1:78407855-78407874 None:intergenic 40.0%
! ATTCTCACCTCAAAGCACTT+GGG - chr4_1:78407948-78407967 None:intergenic 40.0%
GATATATGACTGCCCAAAAG+TGG + chr4_1:78407968-78407987 Msa0574720:CDS 40.0%
!! TCCTATTGCAGTCCACTTTT+GGG - chr4_1:78407983-78408002 None:intergenic 40.0%
! TTCCTATTGCAGTCCACTTT+TGG - chr4_1:78407984-78408003 None:intergenic 40.0%
!! AAAAGTGGACTGCAATAGGA+AGG + chr4_1:78407983-78408002 Msa0574720:CDS 40.0%
ATTCCAAGGTGTGAAAACTG+TGG + chr4_1:78408044-78408063 Msa0574720:CDS 40.0%
! CGCAACTTCTTCAAGTTCTT+CGG - chr4_1:78408084-78408103 None:intergenic 40.0%
GAAGCAGTAACCAACATTCA+AGG - chr4_1:78408124-78408143 None:intergenic 40.0%
GTGTGTGACGAAAATTCTCA+CGG + chr4_1:78408230-78408249 Msa0574720:CDS 40.0%
AAGCATGACCGAAATCATGT+TGG - chr4_1:78408441-78408460 None:intergenic 40.0%
GTTTCCCAGCCACATAGATA+CGG + chr4_1:78406779-78406798 Msa0574720:five_prime_UTR 45.0%
TTGTTCTGCTTCCTCAACTG+TGG - chr4_1:78406985-78407004 None:intergenic 45.0%
AGTTGAGGAAGCAGAACAAG+AGG + chr4_1:78406986-78407005 Msa0574720:CDS 45.0%
GTTGAGGAAGCAGAACAAGA+GGG + chr4_1:78406987-78407006 Msa0574720:CDS 45.0%
AGAAACATGGCTTCATGAGG+AGG - chr4_1:78407014-78407033 None:intergenic 45.0%
TCCTCATGAAGCCATGTTTC+TGG + chr4_1:78407013-78407032 Msa0574720:CDS 45.0%
ACCAGAAACATGGCTTCATG+AGG - chr4_1:78407017-78407036 None:intergenic 45.0%
TGCATCCACATTACTGACCA+TGG + chr4_1:78407236-78407255 Msa0574720:CDS 45.0%
GTAGTCCATGGTCAGTAATG+TGG - chr4_1:78407244-78407263 None:intergenic 45.0%
CAACAACCCTTTGTAGTCCA+TGG - chr4_1:78407256-78407275 None:intergenic 45.0%
AGGAATAGTCTCTGTGCTTG+CGG - chr4_1:78407483-78407502 None:intergenic 45.0%
CAAGCACAGAGACTATTCCT+AGG + chr4_1:78407483-78407502 Msa0574720:intron 45.0%
GCAACCTTACCATTGTGTTG+AGG + chr4_1:78407538-78407557 Msa0574720:intron 45.0%
CTTCATATACCAGCATGCAC+TGG + chr4_1:78407672-78407691 Msa0574720:CDS 45.0%
! TGGTTAAGCAGTTGGCTCTT+TGG - chr4_1:78407740-78407759 None:intergenic 45.0%
ACTCAAGGTGGTTAAGCAGT+TGG - chr4_1:78407748-78407767 None:intergenic 45.0%
! TTGGCTCGTCTTCTAATGGA+AGG - chr4_1:78407836-78407855 None:intergenic 45.0%
!! TGTGTTGGCTCGTCTTCTAA+TGG - chr4_1:78407840-78407859 None:intergenic 45.0%
GAATTGTCCCAAGTGCTTTG+AGG + chr4_1:78407938-78407957 Msa0574720:CDS 45.0%
! CATTCTCACCTCAAAGCACT+TGG - chr4_1:78407949-78407968 None:intergenic 45.0%
!! GAAAACTGTGGTGGATGTGT+TGG + chr4_1:78408056-78408075 Msa0574720:CDS 45.0%
GAACTTGAAGAAGTTGCGTG+CGG + chr4_1:78408086-78408105 Msa0574720:CDS 45.0%
GTAAGCAGCACACAAATTGG+TGG + chr4_1:78408174-78408193 Msa0574720:CDS 45.0%
GTTTCCCAGCCACATAGATA+CGG + chr4_1:78406779-78406798 Msa0574720:five_prime_UTR 45.0%
TTGTTCTGCTTCCTCAACTG+TGG - chr4_1:78406985-78407004 None:intergenic 45.0%
AGTTGAGGAAGCAGAACAAG+AGG + chr4_1:78406986-78407005 Msa0574720:CDS 45.0%
GTTGAGGAAGCAGAACAAGA+GGG + chr4_1:78406987-78407006 Msa0574720:CDS 45.0%
AGAAACATGGCTTCATGAGG+AGG - chr4_1:78407014-78407033 None:intergenic 45.0%
TCCTCATGAAGCCATGTTTC+TGG + chr4_1:78407013-78407032 Msa0574720:CDS 45.0%
ACCAGAAACATGGCTTCATG+AGG - chr4_1:78407017-78407036 None:intergenic 45.0%
TGCATCCACATTACTGACCA+TGG + chr4_1:78407236-78407255 Msa0574720:CDS 45.0%
GTAGTCCATGGTCAGTAATG+TGG - chr4_1:78407244-78407263 None:intergenic 45.0%
CAACAACCCTTTGTAGTCCA+TGG - chr4_1:78407256-78407275 None:intergenic 45.0%
AGGAATAGTCTCTGTGCTTG+CGG - chr4_1:78407483-78407502 None:intergenic 45.0%
CAAGCACAGAGACTATTCCT+AGG + chr4_1:78407483-78407502 Msa0574720:intron 45.0%
GCAACCTTACCATTGTGTTG+AGG + chr4_1:78407538-78407557 Msa0574720:intron 45.0%
CTTCATATACCAGCATGCAC+TGG + chr4_1:78407672-78407691 Msa0574720:CDS 45.0%
! TGGTTAAGCAGTTGGCTCTT+TGG - chr4_1:78407740-78407759 None:intergenic 45.0%
ACTCAAGGTGGTTAAGCAGT+TGG - chr4_1:78407748-78407767 None:intergenic 45.0%
! TTGGCTCGTCTTCTAATGGA+AGG - chr4_1:78407836-78407855 None:intergenic 45.0%
!! TGTGTTGGCTCGTCTTCTAA+TGG - chr4_1:78407840-78407859 None:intergenic 45.0%
GAATTGTCCCAAGTGCTTTG+AGG + chr4_1:78407938-78407957 Msa0574720:CDS 45.0%
! CATTCTCACCTCAAAGCACT+TGG - chr4_1:78407949-78407968 None:intergenic 45.0%
!! GAAAACTGTGGTGGATGTGT+TGG + chr4_1:78408056-78408075 Msa0574720:CDS 45.0%
GAACTTGAAGAAGTTGCGTG+CGG + chr4_1:78408086-78408105 Msa0574720:CDS 45.0%
GTAAGCAGCACACAAATTGG+TGG + chr4_1:78408174-78408193 Msa0574720:CDS 45.0%
GCATCCGTATCTATGTGGCT+GGG - chr4_1:78406786-78406805 None:intergenic 50.0%
TGCATCCGTATCTATGTGGC+TGG - chr4_1:78406787-78406806 None:intergenic 50.0%
TGAAACGAGCACCACAGTTG+AGG + chr4_1:78406971-78406990 Msa0574720:CDS 50.0%
AGCTTACCTTCGAGTGTACG+AGG + chr4_1:78407040-78407059 Msa0574720:CDS 50.0%
GGATAGTGAGCAAAGGGATG+TGG + chr4_1:78407415-78407434 Msa0574720:intron 50.0%
AATAGTCTCTGTGCTTGCGG+TGG - chr4_1:78407480-78407499 None:intergenic 50.0%
TTGGCTACCTCTGTGTCACA+AGG + chr4_1:78407513-78407532 Msa0574720:intron 50.0%
! AACCCCCGAAGGAGTTCTAA+CGG + chr4_1:78407698-78407717 Msa0574720:CDS 50.0%
ACAGCCGTTAGAACTCCTTC+GGG - chr4_1:78407705-78407724 None:intergenic 50.0%
CACAGCCGTTAGAACTCCTT+CGG - chr4_1:78407706-78407725 None:intergenic 50.0%
!! GCCCAAAAGTGGACTGCAAT+AGG + chr4_1:78407979-78407998 Msa0574720:CDS 50.0%
AATCGCGCCAAGCTCAATGT+AGG + chr4_1:78408006-78408025 Msa0574720:CDS 50.0%
ATCGCGCCAAGCTCAATGTA+GGG + chr4_1:78408007-78408026 Msa0574720:CDS 50.0%
TGCATCCCCTACATTGAGCT+TGG - chr4_1:78408016-78408035 None:intergenic 50.0%
! CCACCACAGTTTTCACACCT+TGG - chr4_1:78408050-78408069 None:intergenic 50.0%
CCAAGGTGTGAAAACTGTGG+TGG + chr4_1:78408047-78408066 Msa0574720:CDS 50.0%
GCATCCGTATCTATGTGGCT+GGG - chr4_1:78406786-78406805 None:intergenic 50.0%
TGCATCCGTATCTATGTGGC+TGG - chr4_1:78406787-78406806 None:intergenic 50.0%
TGAAACGAGCACCACAGTTG+AGG + chr4_1:78406971-78406990 Msa0574720:CDS 50.0%
AGCTTACCTTCGAGTGTACG+AGG + chr4_1:78407040-78407059 Msa0574720:CDS 50.0%
GGATAGTGAGCAAAGGGATG+TGG + chr4_1:78407415-78407434 Msa0574720:intron 50.0%
AATAGTCTCTGTGCTTGCGG+TGG - chr4_1:78407480-78407499 None:intergenic 50.0%
TTGGCTACCTCTGTGTCACA+AGG + chr4_1:78407513-78407532 Msa0574720:intron 50.0%
! AACCCCCGAAGGAGTTCTAA+CGG + chr4_1:78407698-78407717 Msa0574720:CDS 50.0%
ACAGCCGTTAGAACTCCTTC+GGG - chr4_1:78407705-78407724 None:intergenic 50.0%
CACAGCCGTTAGAACTCCTT+CGG - chr4_1:78407706-78407725 None:intergenic 50.0%
!! GCCCAAAAGTGGACTGCAAT+AGG + chr4_1:78407979-78407998 Msa0574720:CDS 50.0%
AATCGCGCCAAGCTCAATGT+AGG + chr4_1:78408006-78408025 Msa0574720:CDS 50.0%
ATCGCGCCAAGCTCAATGTA+GGG + chr4_1:78408007-78408026 Msa0574720:CDS 50.0%
TGCATCCCCTACATTGAGCT+TGG - chr4_1:78408016-78408035 None:intergenic 50.0%
! CCACCACAGTTTTCACACCT+TGG - chr4_1:78408050-78408069 None:intergenic 50.0%
CCAAGGTGTGAAAACTGTGG+TGG + chr4_1:78408047-78408066 Msa0574720:CDS 50.0%
GTTGCTGCCTTGTGACACAG+AGG - chr4_1:78407523-78407542 None:intergenic 55.0%
AGCCGTTAGAACTCCTTCGG+GGG - chr4_1:78407703-78407722 None:intergenic 55.0%
CAGCCGTTAGAACTCCTTCG+GGG - chr4_1:78407704-78407723 None:intergenic 55.0%
TCGCGCCAAGCTCAATGTAG+GGG + chr4_1:78408008-78408027 Msa0574720:CDS 55.0%
GTTGCTGCCTTGTGACACAG+AGG - chr4_1:78407523-78407542 None:intergenic 55.0%
AGCCGTTAGAACTCCTTCGG+GGG - chr4_1:78407703-78407722 None:intergenic 55.0%
CAGCCGTTAGAACTCCTTCG+GGG - chr4_1:78407704-78407723 None:intergenic 55.0%
TCGCGCCAAGCTCAATGTAG+GGG + chr4_1:78408008-78408027 Msa0574720:CDS 55.0%
GGGGTTATCCCAGTGCATGC+TGG - chr4_1:78407684-78407703 None:intergenic 60.0%
TGCACTGGGATAACCCCCGA+AGG + chr4_1:78407687-78407706 Msa0574720:CDS 60.0%
GGGGTTATCCCAGTGCATGC+TGG - chr4_1:78407684-78407703 None:intergenic 60.0%
TGCACTGGGATAACCCCCGA+AGG + chr4_1:78407687-78407706 Msa0574720:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 78406748 78408543 78406748 ID=Msa0574720;Name=Msa0574720
chr4_1 mRNA 78406748 78408543 78406748 ID=Msa0574720-mRNA-1;Parent=Msa0574720;Name=Msa0574720-mRNA-1;_AED=0.00;_eAED=0.00;_QI=194|1|1|1|1|1|2|266|320
chr4_1 exon 78406748 78407298 78406748 ID=Msa0574720-mRNA-1:exon:27807;Parent=Msa0574720-mRNA-1
chr4_1 exon 78407672 78408543 78407672 ID=Msa0574720-mRNA-1:exon:27808;Parent=Msa0574720-mRNA-1
chr4_1 five_prime_UTR 78406748 78406941 78406748 ID=Msa0574720-mRNA-1:five_prime_utr;Parent=Msa0574720-mRNA-1
chr4_1 CDS 78406942 78407298 78406942 ID=Msa0574720-mRNA-1:cds;Parent=Msa0574720-mRNA-1
chr4_1 CDS 78407672 78408277 78407672 ID=Msa0574720-mRNA-1:cds;Parent=Msa0574720-mRNA-1
chr4_1 three_prime_UTR 78408278 78408543 78408278 ID=Msa0574720-mRNA-1:three_prime_utr;Parent=Msa0574720-mRNA-1
Gene Sequence

>Msa0574720

ATGGAAGAGTCTTGCAAAGCTTCTTCTTCTGAAACGAGCACCACAGTTGAGGAAGCAGAACAAGAGGGTCCTCCTCATGAAGCCATGTTTCTGGTGATAGCTTACCTTCGAGTGTACGAGGTTTTAACCATGTCTTGTGTTTGCACATCACTGAGATATGCAGTCAACAATGATGTATTGCTATGGCTTAAAATTATTGTTGAAACCCCTCTCAATTCAAGACTCTCAGATGACACACTATTGAAGATCACATCCAAAGCAAATGGTAGGCTTCAAACACTTGCTCTCATGAACTGCATCCACATTACTGACCATGGACTACAAAGGGTTGTTGAACAAAACCTCTTTATTGAAGAGCTTCATATACCAGCATGCACTGGGATAACCCCCGAAGGAGTTCTAACGGCTGTGAAAACACTCTGCCAAAGAGCCAACTGCTTAACCACCTTGAGTTTAAACGGCATTTACAACCTACAAAACGATCATCTTGACGTGCTTACCTCAAACCTCAGAAAAAACCTTCCATTAGAAGACGAGCCAACACAACAGCCTATCTACTATCATAAACGTGGCAGCGTTTCAGCATTCAAGCACAACGAAAACAAGAAGATTATTGATTTGGAGAATTGTCCCAAGTGCTTTGAGGTGAGAATGATATATGACTGCCCAAAAGTGGACTGCAATAGGAAGGAATCGCGCCAAGCTCAATGTAGGGGATGCATATTTTGTATTCCAAGGTGTGAAAACTGTGGTGGATGTGTTGGATCCGAAGAACTTGAAGAAGTTGCGTGCGGAGATTTCTTGTGCCTTGAATGTTGGTTACTGCTTCCCAAGTGCAATTTCTGTAACAAACCATATTGTAAGCAGCACACAAATTGGTGGCGCACTTCTTCAGAATCTTCATTTCTCTGCAGAGTGTGTGACGAAAATTCTCACGGATATACATACACTGATGTTCTATAA
Protein sequence

>Msa0574720

MEESCKASSSETSTTVEEAEQEGPPHEAMFLVIAYLRVYEVLTMSCVCTSLRYAVNNDVLLWLKIIVETPLNSRLSDDTLLKITSKANGRLQTLALMNCIHITDHGLQRVVEQNLFIEELHIPACTGITPEGVLTAVKTLCQRANCLTTLSLNGIYNLQNDHLDVLTSNLRKNLPLEDEPTQQPIYYHKRGSVSAFKHNENKKIIDLENCPKCFEVRMIYDCPKVDCNRKESRQAQCRGCIFCIPRCENCGGCVGSEELEEVACGDFLCLECWLLLPKCNFCNKPYCKQHTNWWRTSSESSFLCRVCDENSHGYTYTDVL*