AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1071770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1071770 MtrunA17_Chr7g0253661 88.406 207 18 2 1 206 1 202 2.82e-114 324
Msa1071770 MtrunA17_Chr6g0481571 40.000 105 55 2 1 102 1 100 1.62e-19 81.6
Msa1071770 MtrunA17_Chr5g0440291 38.947 95 53 1 8 102 11 100 2.81e-17 77.4
Msa1071770 MtrunA17_Chr5g0440311 35.200 125 67 4 8 123 10 129 3.42e-16 74.7
Msa1071770 MtrunA17_Chr5g0440321 31.111 135 80 4 8 136 10 137 5.50e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1071770 AT1G54400.1 40.566 106 57 2 1 106 5 104 3.45e-20 84.3
Msa1071770 AT2G27140.1 33.945 109 66 2 5 112 7 110 5.02e-16 73.9
Msa1071770 AT5G20970.1 34.615 104 63 1 9 112 8 106 7.37e-15 71.2
Msa1071770 AT5G04890.1 32.692 104 60 3 11 109 16 114 1.07e-13 69.3

Find 28 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCTTCATTGCAATATTT+TGG 0.150093 7_2:-68612716 Msa1071760:intron
TCTGGCATTTGTTCTTCTTC+TGG 0.224256 7_2:-68612815 Msa1071760:intron
ACATATCAGAGTTTGGAATT+TGG 0.240193 7_2:+68612872 Msa1071770:CDS
ACCGTTGAGCTTCATCTTCC+AGG 0.311460 7_2:+68612376 Msa1071770:CDS
TACACAACAGACAACAAGTT+TGG 0.336570 7_2:+68612941 Msa1071770:CDS
AGAGGTTGCTCTTAAATTCA+TGG 0.354880 7_2:+68612988 Msa1071770:CDS
GTCCATTTGATGGAACTAAA+TGG 0.359566 7_2:+68612666 Msa1071770:CDS
ATATCCTCATCTTCAAGTTC+TGG 0.365913 7_2:-68612833 Msa1071760:intron
TTCCATTTAGTTCCATCAAA+TGG 0.385038 7_2:-68612668 Msa1071760:intron
CGAATTCAAATCAACCATCT+TGG 0.396315 7_2:+68612620 Msa1071770:CDS
AGTGGAGAGCGTCCATTTGA+TGG 0.411520 7_2:+68612656 Msa1071770:CDS
GGTTGTGATAATTGTTGTAA+TGG 0.450646 7_2:+68613009 Msa1071770:CDS
AACACAAACATGCACATAAC+CGG 0.451506 7_2:+68612299 Msa1071770:CDS
TCTTCTTCTGGTTCCTCTTG+AGG 0.457813 7_2:-68612803 Msa1071760:intron
GGTTATTGTAAATTATCTTG+TGG 0.496692 7_2:+68613030 Msa1071770:CDS
TGCAATGAAGATGCCATTCG+TGG 0.501501 7_2:+68612725 Msa1071770:CDS
TATCAGAGTTTGGAATTTGG+AGG 0.521412 7_2:+68612875 Msa1071770:CDS
ATACATGAAATTGACATGCC+TGG 0.528684 7_2:-68612394 Msa1071760:intron
TTGCATGAAATTGCCACGAA+TGG 0.562966 7_2:-68612738 Msa1071760:intron
AGCAAAACCGTCATGGCTCA+AGG 0.573165 7_2:+68613058 Msa1071770:CDS
GATCAAAATCTTCATAAGAC+CGG 0.586407 7_2:-68612318 Msa1071760:intron
AATGCCAGAACTTGAAGATG+AGG 0.591611 7_2:+68612829 Msa1071770:CDS
AGTTGATCTGATACCTCAAG+AGG 0.599832 7_2:+68612790 Msa1071770:CDS
TGATTGACCTTGAGCCATGA+CGG 0.602155 7_2:-68613065 Msa1071760:intron
GATGAAGCTCAACGGTGTCA+CGG 0.610092 7_2:-68612369 Msa1071760:intron
GCCTGGAAGATGAAGCTCAA+CGG 0.610375 7_2:-68612377 Msa1071760:intron
GGATATGAGCAAAACCGTCA+TGG 0.621622 7_2:+68613051 Msa1071770:CDS
TGATGTAGAAACTACTAGAG+AGG 0.643103 7_2:+68612970 Msa1071770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATGTATCTAAATTATATA+TGG + chr7_2:68612409-68612428 Msa1071770:intron 15.0%
!!! CATTGTTTTTGTTAAATTTA+CGG - chr7_2:68612512-68612531 Msa1071760:intron 15.0%
!! TCATGTATCTAAATTATATA+TGG + chr7_2:68612409-68612428 Msa1071770:intron 15.0%
!!! CATTGTTTTTGTTAAATTTA+CGG - chr7_2:68612512-68612531 Msa1071760:intron 15.0%
!! AAATGAAATTGAACTAAACA+AGG - chr7_2:68612436-68612455 Msa1071760:intron 20.0%
!! AAATGAAATTGAACTAAACA+AGG - chr7_2:68612436-68612455 Msa1071760:intron 20.0%
!!! ATTTTGATCCTGTTTTTAAG+TGG + chr7_2:68612332-68612351 Msa1071770:CDS 25.0%
! GAAAAATACATATCAGAGTT+TGG + chr7_2:68612865-68612884 Msa1071770:CDS 25.0%
! GGTTATTGTAAATTATCTTG+TGG + chr7_2:68613030-68613049 Msa1071770:CDS 25.0%
!!! ATTTTGATCCTGTTTTTAAG+TGG + chr7_2:68612332-68612351 Msa1071770:CDS 25.0%
! GAAAAATACATATCAGAGTT+TGG + chr7_2:68612865-68612884 Msa1071770:CDS 25.0%
! GGTTATTGTAAATTATCTTG+TGG + chr7_2:68613030-68613049 Msa1071770:CDS 25.0%
GATCAAAATCTTCATAAGAC+CGG - chr7_2:68612321-68612340 Msa1071760:intron 30.0%
!!! TTAGACTTTTTTCACAGACT+AGG + chr7_2:68612535-68612554 Msa1071770:intron 30.0%
!!! TAGACTTTTTTCACAGACTA+GGG + chr7_2:68612536-68612555 Msa1071770:intron 30.0%
!! GCTTTATTTGAGAGTTATGA+GGG + chr7_2:68612558-68612577 Msa1071770:intron 30.0%
! CTTTATTTGAGAGTTATGAG+GGG + chr7_2:68612559-68612578 Msa1071770:intron 30.0%
!!! GGGGTTATTAATTTGTTTTC+AGG + chr7_2:68612578-68612597 Msa1071770:intron 30.0%
!!! AATCAACCATCTTGGTTTTT+TGG + chr7_2:68612628-68612647 Msa1071770:CDS 30.0%
TTATCACCAAAAAACCAAGA+TGG - chr7_2:68612637-68612656 Msa1071760:intron 30.0%
! TTCCATTTAGTTCCATCAAA+TGG - chr7_2:68612671-68612690 Msa1071760:intron 30.0%
! GCATCTTCATTGCAATATTT+TGG - chr7_2:68612719-68612738 Msa1071760:intron 30.0%
!!! GGTATCAGATCAACTTTTTT+TGG - chr7_2:68612785-68612804 Msa1071760:intron 30.0%
! ACATATCAGAGTTTGGAATT+TGG + chr7_2:68612872-68612891 Msa1071770:CDS 30.0%
GGTTGTGATAATTGTTGTAA+TGG + chr7_2:68613009-68613028 Msa1071770:CDS 30.0%
GATCAAAATCTTCATAAGAC+CGG - chr7_2:68612321-68612340 Msa1071760:intron 30.0%
!!! TTAGACTTTTTTCACAGACT+AGG + chr7_2:68612535-68612554 Msa1071770:intron 30.0%
!!! TAGACTTTTTTCACAGACTA+GGG + chr7_2:68612536-68612555 Msa1071770:intron 30.0%
!! GCTTTATTTGAGAGTTATGA+GGG + chr7_2:68612558-68612577 Msa1071770:intron 30.0%
! CTTTATTTGAGAGTTATGAG+GGG + chr7_2:68612559-68612578 Msa1071770:intron 30.0%
!!! GGGGTTATTAATTTGTTTTC+AGG + chr7_2:68612578-68612597 Msa1071770:intron 30.0%
!!! AATCAACCATCTTGGTTTTT+TGG + chr7_2:68612628-68612647 Msa1071770:CDS 30.0%
TTATCACCAAAAAACCAAGA+TGG - chr7_2:68612637-68612656 Msa1071760:intron 30.0%
! TTCCATTTAGTTCCATCAAA+TGG - chr7_2:68612671-68612690 Msa1071760:intron 30.0%
! GCATCTTCATTGCAATATTT+TGG - chr7_2:68612719-68612738 Msa1071760:intron 30.0%
!!! GGTATCAGATCAACTTTTTT+TGG - chr7_2:68612785-68612804 Msa1071760:intron 30.0%
! ACATATCAGAGTTTGGAATT+TGG + chr7_2:68612872-68612891 Msa1071770:CDS 30.0%
GGTTGTGATAATTGTTGTAA+TGG + chr7_2:68613009-68613028 Msa1071770:CDS 30.0%
AACACAAACATGCACATAAC+CGG + chr7_2:68612299-68612318 Msa1071770:CDS 35.0%
ATACATGAAATTGACATGCC+TGG - chr7_2:68612397-68612416 Msa1071760:intron 35.0%
!! GGCTTTATTTGAGAGTTATG+AGG + chr7_2:68612557-68612576 Msa1071770:intron 35.0%
CGAATTCAAATCAACCATCT+TGG + chr7_2:68612620-68612639 Msa1071770:CDS 35.0%
!!! CTTGGTTTTTTGGTGATAAG+TGG + chr7_2:68612638-68612657 Msa1071770:CDS 35.0%
GTCCATTTGATGGAACTAAA+TGG + chr7_2:68612666-68612685 Msa1071770:CDS 35.0%
! ATATCCTCATCTTCAAGTTC+TGG - chr7_2:68612836-68612855 Msa1071760:intron 35.0%
! TATCAGAGTTTGGAATTTGG+AGG + chr7_2:68612875-68612894 Msa1071770:CDS 35.0%
TACACAACAGACAACAAGTT+TGG + chr7_2:68612941-68612960 Msa1071770:CDS 35.0%
TGATGTAGAAACTACTAGAG+AGG + chr7_2:68612970-68612989 Msa1071770:CDS 35.0%
AGAGGTTGCTCTTAAATTCA+TGG + chr7_2:68612988-68613007 Msa1071770:CDS 35.0%
AACACAAACATGCACATAAC+CGG + chr7_2:68612299-68612318 Msa1071770:CDS 35.0%
ATACATGAAATTGACATGCC+TGG - chr7_2:68612397-68612416 Msa1071760:intron 35.0%
!! GGCTTTATTTGAGAGTTATG+AGG + chr7_2:68612557-68612576 Msa1071770:intron 35.0%
CGAATTCAAATCAACCATCT+TGG + chr7_2:68612620-68612639 Msa1071770:CDS 35.0%
!!! CTTGGTTTTTTGGTGATAAG+TGG + chr7_2:68612638-68612657 Msa1071770:CDS 35.0%
GTCCATTTGATGGAACTAAA+TGG + chr7_2:68612666-68612685 Msa1071770:CDS 35.0%
! ATATCCTCATCTTCAAGTTC+TGG - chr7_2:68612836-68612855 Msa1071760:intron 35.0%
! TATCAGAGTTTGGAATTTGG+AGG + chr7_2:68612875-68612894 Msa1071770:CDS 35.0%
TACACAACAGACAACAAGTT+TGG + chr7_2:68612941-68612960 Msa1071770:CDS 35.0%
TGATGTAGAAACTACTAGAG+AGG + chr7_2:68612970-68612989 Msa1071770:CDS 35.0%
AGAGGTTGCTCTTAAATTCA+TGG + chr7_2:68612988-68613007 Msa1071770:CDS 35.0%
TCTCTACGCCACTTAAAAAC+AGG - chr7_2:68612343-68612362 Msa1071760:intron 40.0%
TTGCATGAAATTGCCACGAA+TGG - chr7_2:68612741-68612760 Msa1071760:intron 40.0%
AGTTGATCTGATACCTCAAG+AGG + chr7_2:68612790-68612809 Msa1071770:CDS 40.0%
TCTGGCATTTGTTCTTCTTC+TGG - chr7_2:68612818-68612837 Msa1071760:intron 40.0%
AATGCCAGAACTTGAAGATG+AGG + chr7_2:68612829-68612848 Msa1071770:CDS 40.0%
TCTCTACGCCACTTAAAAAC+AGG - chr7_2:68612343-68612362 Msa1071760:intron 40.0%
TTGCATGAAATTGCCACGAA+TGG - chr7_2:68612741-68612760 Msa1071760:intron 40.0%
AGTTGATCTGATACCTCAAG+AGG + chr7_2:68612790-68612809 Msa1071770:CDS 40.0%
TCTGGCATTTGTTCTTCTTC+TGG - chr7_2:68612818-68612837 Msa1071760:intron 40.0%
AATGCCAGAACTTGAAGATG+AGG + chr7_2:68612829-68612848 Msa1071770:CDS 40.0%
TGCAATGAAGATGCCATTCG+TGG + chr7_2:68612725-68612744 Msa1071770:CDS 45.0%
!! TCTTCTTCTGGTTCCTCTTG+AGG - chr7_2:68612806-68612825 Msa1071760:intron 45.0%
GGATATGAGCAAAACCGTCA+TGG + chr7_2:68613051-68613070 Msa1071770:CDS 45.0%
TGATTGACCTTGAGCCATGA+CGG - chr7_2:68613068-68613087 Msa1071760:intron 45.0%
TGCAATGAAGATGCCATTCG+TGG + chr7_2:68612725-68612744 Msa1071770:CDS 45.0%
!! TCTTCTTCTGGTTCCTCTTG+AGG - chr7_2:68612806-68612825 Msa1071760:intron 45.0%
GGATATGAGCAAAACCGTCA+TGG + chr7_2:68613051-68613070 Msa1071770:CDS 45.0%
TGATTGACCTTGAGCCATGA+CGG - chr7_2:68613068-68613087 Msa1071760:intron 45.0%
! GATGAAGCTCAACGGTGTCA+CGG - chr7_2:68612372-68612391 Msa1071760:intron 50.0%
ACCGTTGAGCTTCATCTTCC+AGG + chr7_2:68612376-68612395 Msa1071770:CDS 50.0%
GCCTGGAAGATGAAGCTCAA+CGG - chr7_2:68612380-68612399 Msa1071760:intron 50.0%
!! AGTGGAGAGCGTCCATTTGA+TGG + chr7_2:68612656-68612675 Msa1071770:CDS 50.0%
AGCAAAACCGTCATGGCTCA+AGG + chr7_2:68613058-68613077 Msa1071770:CDS 50.0%
! GATGAAGCTCAACGGTGTCA+CGG - chr7_2:68612372-68612391 Msa1071760:intron 50.0%
ACCGTTGAGCTTCATCTTCC+AGG + chr7_2:68612376-68612395 Msa1071770:CDS 50.0%
GCCTGGAAGATGAAGCTCAA+CGG - chr7_2:68612380-68612399 Msa1071760:intron 50.0%
!! AGTGGAGAGCGTCCATTTGA+TGG + chr7_2:68612656-68612675 Msa1071770:CDS 50.0%
AGCAAAACCGTCATGGCTCA+AGG + chr7_2:68613058-68613077 Msa1071770:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 68612295 68613102 68612295 ID=Msa1071770;Name=Msa1071770
chr7_2 mRNA 68612295 68613102 68612295 ID=Msa1071770-mRNA-1;Parent=Msa1071770;Name=Msa1071770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|206
chr7_2 exon 68612295 68612412 68612295 ID=Msa1071770-mRNA-1:exon:15043;Parent=Msa1071770-mRNA-1
chr7_2 exon 68612600 68613102 68612600 ID=Msa1071770-mRNA-1:exon:15044;Parent=Msa1071770-mRNA-1
chr7_2 CDS 68612295 68612412 68612295 ID=Msa1071770-mRNA-1:cds;Parent=Msa1071770-mRNA-1
chr7_2 CDS 68612600 68613102 68612600 ID=Msa1071770-mRNA-1:cds;Parent=Msa1071770-mRNA-1
Gene Sequence

>Msa1071770

ATGGAACACAAACATGCACATAACCGGTCTTATGAAGATTTTGATCCTGTTTTTAAGTGGCGTAGAGAACAAGACCGTGACACCGTTGAGCTTCATCTTCCAGGCATGTCAATTTCATGTTTTAAGAGAGAGCAAATACGAATTCAAATCAACCATCTTGGTTTTTTGGTGATAAGTGGAGAGCGTCCATTTGATGGAACTAAATGGAAACGTTTCAAGAAAGAATTTGAACTTCCAAAATATTGCAATGAAGATGCCATTCGTGGCAATTTCATGCAAAACATTCTTTCAGTAGTATTGCCAAAAAAAGTTGATCTGATACCTCAAGAGGAACCAGAAGAAGAACAAATGCCAGAACTTGAAGATGAGGATATATATCAAGAGAAAAATACATATCAGAGTTTGGAATTTGGAGGAAGAAATAGAGAAGCAGAGATTGAAAAACTATCAGAATATAATTACACAACAGACAACAAGTTTGGAGAAAATGATGTAGAAACTACTAGAGAGGTTGCTCTTAAATTCATGGTTGTGATAATTGTTGTAATGGTTATTGTAAATTATCTTGTGGATATGAGCAAAACCGTCATGGCTCAAGGTCAATCATATTTTCAAAATTAG

Protein sequence

>Msa1071770

MEHKHAHNRSYEDFDPVFKWRREQDRDTVELHLPGMSISCFKREQIRIQINHLGFLVISGERPFDGTKWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEPEEEQMPELEDEDIYQEKNTYQSLEFGGRNREAEIEKLSEYNYTTDNKFGENDVETTREVALKFMVVIIVVMVIVNYLVDMSKTVMAQGQSYFQN*