AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1158830


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158830 MtrunA17_Chr7g0253661 88.406 207 18 2 1 206 1 202 2.82e-114 324
Msa1158830 MtrunA17_Chr6g0481571 40.000 105 55 2 1 102 1 100 1.62e-19 81.6
Msa1158830 MtrunA17_Chr5g0440291 38.947 95 53 1 8 102 11 100 2.81e-17 77.4
Msa1158830 MtrunA17_Chr5g0440311 35.200 125 67 4 8 123 10 129 3.42e-16 74.7
Msa1158830 MtrunA17_Chr5g0440321 31.111 135 80 4 8 136 10 137 5.50e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1158830 AT1G54400.1 40.566 106 57 2 1 106 5 104 3.45e-20 84.3
Msa1158830 AT2G27140.1 33.945 109 66 2 5 112 7 110 5.02e-16 73.9
Msa1158830 AT5G20970.1 34.615 104 63 1 9 112 8 106 7.37e-15 71.2
Msa1158830 AT5G04890.1 32.692 104 60 3 11 109 16 114 1.07e-13 69.3

Find 28 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATCTTCATTGCAATATTT+TGG 0.150093 7_4:-48253113 Msa1158820:intron
TCTGGCATTTGTTCTTCTTC+TGG 0.224256 7_4:-48253212 Msa1158820:intron
ACATATCAGAGTTTGGAATT+TGG 0.240193 7_4:+48253269 Msa1158830:CDS
ACCGTTGAGCTTCATCTTCC+AGG 0.311460 7_4:+48252773 Msa1158830:CDS
TACACAACAGACAACAAGTT+TGG 0.336570 7_4:+48253338 Msa1158830:CDS
AGAGGTTGCTCTTAAATTCA+TGG 0.354880 7_4:+48253385 Msa1158830:CDS
GTCCATTTGATGGAACTAAA+TGG 0.359566 7_4:+48253063 Msa1158830:CDS
ATATCCTCATCTTCAAGTTC+TGG 0.365913 7_4:-48253230 Msa1158820:intron
TTCCATTTAGTTCCATCAAA+TGG 0.385038 7_4:-48253065 Msa1158820:intron
CGAATTCAAATCAACCATCT+TGG 0.396315 7_4:+48253017 Msa1158830:CDS
AGTGGAGAGCGTCCATTTGA+TGG 0.411520 7_4:+48253053 Msa1158830:CDS
GGTTGTGATAATTGTTGTAA+TGG 0.450646 7_4:+48253406 Msa1158830:CDS
AACACAAACATGCACATAAC+CGG 0.451506 7_4:+48252696 Msa1158830:CDS
TCTTCTTCTGGTTCCTCTTG+AGG 0.457813 7_4:-48253200 Msa1158820:intron
GGTTATTGTAAATTATCTTG+TGG 0.496692 7_4:+48253427 Msa1158830:CDS
TGCAATGAAGATGCCATTCG+TGG 0.501501 7_4:+48253122 Msa1158830:CDS
TATCAGAGTTTGGAATTTGG+AGG 0.521412 7_4:+48253272 Msa1158830:CDS
ATACATGAAATTGACATGCC+TGG 0.528684 7_4:-48252791 Msa1158820:intron
TTGCATGAAATTGCCACGAA+TGG 0.562966 7_4:-48253135 Msa1158820:intron
AGCAAAACCGTCATGGCTCA+AGG 0.573165 7_4:+48253455 Msa1158830:CDS
GATCAAAATCTTCATAAGAC+CGG 0.586407 7_4:-48252715 Msa1158820:intron
AATGCCAGAACTTGAAGATG+AGG 0.591611 7_4:+48253226 Msa1158830:CDS
AGTTGATCTGATACCTCAAG+AGG 0.599832 7_4:+48253187 Msa1158830:CDS
TGATTGACCTTGAGCCATGA+CGG 0.602155 7_4:-48253462 Msa1158820:intron
GATGAAGCTCAACGGTGTCA+CGG 0.610092 7_4:-48252766 Msa1158820:intron
GCCTGGAAGATGAAGCTCAA+CGG 0.610375 7_4:-48252774 Msa1158820:intron
GGATATGAGCAAAACCGTCA+TGG 0.621622 7_4:+48253448 Msa1158830:CDS
TGATGTAGAAACTACTAGAG+AGG 0.643103 7_4:+48253367 Msa1158830:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATGTATCTAAATTATATA+TGG + chr7_4:48252806-48252825 Msa1158830:intron 15.0%
!!! CATTGTTTTTGTTAAATTTA+CGG - chr7_4:48252909-48252928 Msa1158820:intron 15.0%
!! TCATGTATCTAAATTATATA+TGG + chr7_4:48252806-48252825 Msa1158830:intron 15.0%
!!! CATTGTTTTTGTTAAATTTA+CGG - chr7_4:48252909-48252928 Msa1158820:intron 15.0%
!! AAATGAAATTGAACTAAACA+AGG - chr7_4:48252833-48252852 Msa1158820:intron 20.0%
!! AAATGAAATTGAACTAAACA+AGG - chr7_4:48252833-48252852 Msa1158820:intron 20.0%
!!! ATTTTGATCCTGTTTTTAAG+TGG + chr7_4:48252729-48252748 Msa1158830:CDS 25.0%
! GAAAAATACATATCAGAGTT+TGG + chr7_4:48253262-48253281 Msa1158830:CDS 25.0%
! GGTTATTGTAAATTATCTTG+TGG + chr7_4:48253427-48253446 Msa1158830:CDS 25.0%
!!! ATTTTGATCCTGTTTTTAAG+TGG + chr7_4:48252729-48252748 Msa1158830:CDS 25.0%
! GAAAAATACATATCAGAGTT+TGG + chr7_4:48253262-48253281 Msa1158830:CDS 25.0%
! GGTTATTGTAAATTATCTTG+TGG + chr7_4:48253427-48253446 Msa1158830:CDS 25.0%
GATCAAAATCTTCATAAGAC+CGG - chr7_4:48252718-48252737 Msa1158820:intron 30.0%
!!! TTAGACTTTTTTCACAGACT+AGG + chr7_4:48252932-48252951 Msa1158830:intron 30.0%
!!! TAGACTTTTTTCACAGACTA+GGG + chr7_4:48252933-48252952 Msa1158830:intron 30.0%
!! GCTTTATTTGAGAGTTATGA+GGG + chr7_4:48252955-48252974 Msa1158830:intron 30.0%
! CTTTATTTGAGAGTTATGAG+GGG + chr7_4:48252956-48252975 Msa1158830:intron 30.0%
!!! GGGGTTATTAATTTGTTTTC+AGG + chr7_4:48252975-48252994 Msa1158830:intron 30.0%
!!! AATCAACCATCTTGGTTTTT+TGG + chr7_4:48253025-48253044 Msa1158830:CDS 30.0%
TTATCACCAAAAAACCAAGA+TGG - chr7_4:48253034-48253053 Msa1158820:intron 30.0%
! TTCCATTTAGTTCCATCAAA+TGG - chr7_4:48253068-48253087 Msa1158820:intron 30.0%
! GCATCTTCATTGCAATATTT+TGG - chr7_4:48253116-48253135 Msa1158820:intron 30.0%
!!! GGTATCAGATCAACTTTTTT+TGG - chr7_4:48253182-48253201 Msa1158820:intron 30.0%
! ACATATCAGAGTTTGGAATT+TGG + chr7_4:48253269-48253288 Msa1158830:CDS 30.0%
GGTTGTGATAATTGTTGTAA+TGG + chr7_4:48253406-48253425 Msa1158830:CDS 30.0%
GATCAAAATCTTCATAAGAC+CGG - chr7_4:48252718-48252737 Msa1158820:intron 30.0%
!!! TTAGACTTTTTTCACAGACT+AGG + chr7_4:48252932-48252951 Msa1158830:intron 30.0%
!!! TAGACTTTTTTCACAGACTA+GGG + chr7_4:48252933-48252952 Msa1158830:intron 30.0%
!! GCTTTATTTGAGAGTTATGA+GGG + chr7_4:48252955-48252974 Msa1158830:intron 30.0%
! CTTTATTTGAGAGTTATGAG+GGG + chr7_4:48252956-48252975 Msa1158830:intron 30.0%
!!! GGGGTTATTAATTTGTTTTC+AGG + chr7_4:48252975-48252994 Msa1158830:intron 30.0%
!!! AATCAACCATCTTGGTTTTT+TGG + chr7_4:48253025-48253044 Msa1158830:CDS 30.0%
TTATCACCAAAAAACCAAGA+TGG - chr7_4:48253034-48253053 Msa1158820:intron 30.0%
! TTCCATTTAGTTCCATCAAA+TGG - chr7_4:48253068-48253087 Msa1158820:intron 30.0%
! GCATCTTCATTGCAATATTT+TGG - chr7_4:48253116-48253135 Msa1158820:intron 30.0%
!!! GGTATCAGATCAACTTTTTT+TGG - chr7_4:48253182-48253201 Msa1158820:intron 30.0%
! ACATATCAGAGTTTGGAATT+TGG + chr7_4:48253269-48253288 Msa1158830:CDS 30.0%
GGTTGTGATAATTGTTGTAA+TGG + chr7_4:48253406-48253425 Msa1158830:CDS 30.0%
AACACAAACATGCACATAAC+CGG + chr7_4:48252696-48252715 Msa1158830:CDS 35.0%
ATACATGAAATTGACATGCC+TGG - chr7_4:48252794-48252813 Msa1158820:intron 35.0%
!! GGCTTTATTTGAGAGTTATG+AGG + chr7_4:48252954-48252973 Msa1158830:intron 35.0%
CGAATTCAAATCAACCATCT+TGG + chr7_4:48253017-48253036 Msa1158830:CDS 35.0%
!!! CTTGGTTTTTTGGTGATAAG+TGG + chr7_4:48253035-48253054 Msa1158830:CDS 35.0%
GTCCATTTGATGGAACTAAA+TGG + chr7_4:48253063-48253082 Msa1158830:CDS 35.0%
! ATATCCTCATCTTCAAGTTC+TGG - chr7_4:48253233-48253252 Msa1158820:intron 35.0%
! TATCAGAGTTTGGAATTTGG+AGG + chr7_4:48253272-48253291 Msa1158830:CDS 35.0%
TACACAACAGACAACAAGTT+TGG + chr7_4:48253338-48253357 Msa1158830:CDS 35.0%
TGATGTAGAAACTACTAGAG+AGG + chr7_4:48253367-48253386 Msa1158830:CDS 35.0%
AGAGGTTGCTCTTAAATTCA+TGG + chr7_4:48253385-48253404 Msa1158830:CDS 35.0%
AACACAAACATGCACATAAC+CGG + chr7_4:48252696-48252715 Msa1158830:CDS 35.0%
ATACATGAAATTGACATGCC+TGG - chr7_4:48252794-48252813 Msa1158820:intron 35.0%
!! GGCTTTATTTGAGAGTTATG+AGG + chr7_4:48252954-48252973 Msa1158830:intron 35.0%
CGAATTCAAATCAACCATCT+TGG + chr7_4:48253017-48253036 Msa1158830:CDS 35.0%
!!! CTTGGTTTTTTGGTGATAAG+TGG + chr7_4:48253035-48253054 Msa1158830:CDS 35.0%
GTCCATTTGATGGAACTAAA+TGG + chr7_4:48253063-48253082 Msa1158830:CDS 35.0%
! ATATCCTCATCTTCAAGTTC+TGG - chr7_4:48253233-48253252 Msa1158820:intron 35.0%
! TATCAGAGTTTGGAATTTGG+AGG + chr7_4:48253272-48253291 Msa1158830:CDS 35.0%
TACACAACAGACAACAAGTT+TGG + chr7_4:48253338-48253357 Msa1158830:CDS 35.0%
TGATGTAGAAACTACTAGAG+AGG + chr7_4:48253367-48253386 Msa1158830:CDS 35.0%
AGAGGTTGCTCTTAAATTCA+TGG + chr7_4:48253385-48253404 Msa1158830:CDS 35.0%
TCTCTACGCCACTTAAAAAC+AGG - chr7_4:48252740-48252759 Msa1158820:intron 40.0%
TTGCATGAAATTGCCACGAA+TGG - chr7_4:48253138-48253157 Msa1158820:intron 40.0%
AGTTGATCTGATACCTCAAG+AGG + chr7_4:48253187-48253206 Msa1158830:CDS 40.0%
TCTGGCATTTGTTCTTCTTC+TGG - chr7_4:48253215-48253234 Msa1158820:intron 40.0%
AATGCCAGAACTTGAAGATG+AGG + chr7_4:48253226-48253245 Msa1158830:CDS 40.0%
TCTCTACGCCACTTAAAAAC+AGG - chr7_4:48252740-48252759 Msa1158820:intron 40.0%
TTGCATGAAATTGCCACGAA+TGG - chr7_4:48253138-48253157 Msa1158820:intron 40.0%
AGTTGATCTGATACCTCAAG+AGG + chr7_4:48253187-48253206 Msa1158830:CDS 40.0%
TCTGGCATTTGTTCTTCTTC+TGG - chr7_4:48253215-48253234 Msa1158820:intron 40.0%
AATGCCAGAACTTGAAGATG+AGG + chr7_4:48253226-48253245 Msa1158830:CDS 40.0%
TGCAATGAAGATGCCATTCG+TGG + chr7_4:48253122-48253141 Msa1158830:CDS 45.0%
!! TCTTCTTCTGGTTCCTCTTG+AGG - chr7_4:48253203-48253222 Msa1158820:intron 45.0%
GGATATGAGCAAAACCGTCA+TGG + chr7_4:48253448-48253467 Msa1158830:CDS 45.0%
TGATTGACCTTGAGCCATGA+CGG - chr7_4:48253465-48253484 Msa1158820:intron 45.0%
TGCAATGAAGATGCCATTCG+TGG + chr7_4:48253122-48253141 Msa1158830:CDS 45.0%
!! TCTTCTTCTGGTTCCTCTTG+AGG - chr7_4:48253203-48253222 Msa1158820:intron 45.0%
GGATATGAGCAAAACCGTCA+TGG + chr7_4:48253448-48253467 Msa1158830:CDS 45.0%
TGATTGACCTTGAGCCATGA+CGG - chr7_4:48253465-48253484 Msa1158820:intron 45.0%
! GATGAAGCTCAACGGTGTCA+CGG - chr7_4:48252769-48252788 Msa1158820:intron 50.0%
ACCGTTGAGCTTCATCTTCC+AGG + chr7_4:48252773-48252792 Msa1158830:CDS 50.0%
GCCTGGAAGATGAAGCTCAA+CGG - chr7_4:48252777-48252796 Msa1158820:intron 50.0%
!! AGTGGAGAGCGTCCATTTGA+TGG + chr7_4:48253053-48253072 Msa1158830:CDS 50.0%
AGCAAAACCGTCATGGCTCA+AGG + chr7_4:48253455-48253474 Msa1158830:CDS 50.0%
! GATGAAGCTCAACGGTGTCA+CGG - chr7_4:48252769-48252788 Msa1158820:intron 50.0%
ACCGTTGAGCTTCATCTTCC+AGG + chr7_4:48252773-48252792 Msa1158830:CDS 50.0%
GCCTGGAAGATGAAGCTCAA+CGG - chr7_4:48252777-48252796 Msa1158820:intron 50.0%
!! AGTGGAGAGCGTCCATTTGA+TGG + chr7_4:48253053-48253072 Msa1158830:CDS 50.0%
AGCAAAACCGTCATGGCTCA+AGG + chr7_4:48253455-48253474 Msa1158830:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 48252692 48253499 48252692 ID=Msa1158830;Name=Msa1158830
chr7_4 mRNA 48252692 48253499 48252692 ID=Msa1158830-mRNA-1;Parent=Msa1158830;Name=Msa1158830-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|206
chr7_4 exon 48252692 48252809 48252692 ID=Msa1158830-mRNA-1:exon:12987;Parent=Msa1158830-mRNA-1
chr7_4 exon 48252997 48253499 48252997 ID=Msa1158830-mRNA-1:exon:12988;Parent=Msa1158830-mRNA-1
chr7_4 CDS 48252692 48252809 48252692 ID=Msa1158830-mRNA-1:cds;Parent=Msa1158830-mRNA-1
chr7_4 CDS 48252997 48253499 48252997 ID=Msa1158830-mRNA-1:cds;Parent=Msa1158830-mRNA-1
Gene Sequence

>Msa1158830

ATGGAACACAAACATGCACATAACCGGTCTTATGAAGATTTTGATCCTGTTTTTAAGTGGCGTAGAGAACAAGACCGTGACACCGTTGAGCTTCATCTTCCAGGCATGTCAATTTCATGTTTTAAGAGAGAGCAAATACGAATTCAAATCAACCATCTTGGTTTTTTGGTGATAAGTGGAGAGCGTCCATTTGATGGAACTAAATGGAAACGTTTCAAGAAAGAATTTGAACTTCCAAAATATTGCAATGAAGATGCCATTCGTGGCAATTTCATGCAAAACATTCTTTCAGTAGTATTGCCAAAAAAAGTTGATCTGATACCTCAAGAGGAACCAGAAGAAGAACAAATGCCAGAACTTGAAGATGAGGATATATATCAAGAGAAAAATACATATCAGAGTTTGGAATTTGGAGGAAGAAATAGAGAAGCAGAGATTGAAAAACTATCAGAATATAATTACACAACAGACAACAAGTTTGGAGAAAATGATGTAGAAACTACTAGAGAGGTTGCTCTTAAATTCATGGTTGTGATAATTGTTGTAATGGTTATTGTAAATTATCTTGTGGATATGAGCAAAACCGTCATGGCTCAAGGTCAATCATATTTTCAAAATTAG

Protein sequence

>Msa1158830

MEHKHAHNRSYEDFDPVFKWRREQDRDTVELHLPGMSISCFKREQIRIQINHLGFLVISGERPFDGTKWKRFKKEFELPKYCNEDAIRGNFMQNILSVVLPKKVDLIPQEEPEEEQMPELEDEDIYQEKNTYQSLEFGGRNREAEIEKLSEYNYTTDNKFGENDVETTREVALKFMVVIIVVMVIVNYLVDMSKTVMAQGQSYFQN*