AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0581980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581980 MtrunA17_Chr4g0070561 93.464 306 18 1 1 306 1 304 0.0 593
Msa0581980 MtrunA17_Chr5g0394771 53.986 276 113 2 17 292 36 297 5.95e-107 317
Msa0581980 MtrunA17_Chr5g0394891 52.797 286 127 2 13 298 4 281 5.22e-106 313
Msa0581980 MtrunA17_Chr5g0394751 53.676 272 112 2 21 292 8 265 5.75e-105 310
Msa0581980 MtrunA17_Chr3g0144001 54.480 279 113 3 14 291 3 268 2.25e-102 304
Msa0581980 MtrunA17_Chr5g0394861 53.381 281 119 4 21 298 12 283 1.54e-101 302
Msa0581980 MtrunA17_Chr5g0394811 53.480 273 117 3 21 292 121 384 3.88e-100 302
Msa0581980 MtrunA17_Chr5g0394811 43.312 157 69 4 27 164 1 156 2.23e-34 130
Msa0581980 MtrunA17_Chr5g0395841 52.206 272 122 2 21 292 14 277 4.16e-98 293
Msa0581980 MtrunA17_Chr5g0394841 52.190 274 121 3 21 292 12 277 1.07e-97 292
Msa0581980 MtrunA17_Chr5g0394791 50.595 168 73 3 21 187 11 169 2.80e-48 160
Msa0581980 MtrunA17_Chr5g0394801 59.091 88 36 0 186 273 26 113 3.79e-32 119
Msa0581980 MtrunA17_Chr3g0130471 65.116 43 15 0 205 247 1 43 4.69e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0581980 AT2G06050.2 73.220 295 78 1 11 304 2 296 3.24e-163 460
Msa0581980 AT2G06050.1 73.220 295 78 1 11 304 2 296 3.24e-163 460
Msa0581980 AT2G06050.3 73.220 295 78 1 11 304 2 296 3.24e-163 460
Msa0581980 AT1G76690.1 50.871 287 133 2 14 300 7 285 8.91e-104 308
Msa0581980 AT1G76680.1 51.748 286 130 2 14 299 5 282 8.51e-102 303
Msa0581980 AT1G09400.1 50.000 278 125 2 27 304 1 264 6.93e-100 296
Msa0581980 AT1G17990.1 52.874 261 116 2 13 273 1 254 7.10e-100 295
Msa0581980 AT1G18020.1 52.874 261 116 2 13 273 1 254 7.10e-100 295
Msa0581980 AT1G76680.2 47.588 311 130 3 14 299 5 307 4.73e-96 289
Msa0581980 AT1G17990.2 51.172 256 110 3 18 273 6 246 1.38e-91 273
Msa0581980 AT1G09400.2 50.228 219 95 2 86 304 1 205 7.33e-76 233

Find 118 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATAATCCTTGACTATTTC+TGG 0.074699 4_1:+87555003 None:intergenic
GTGGTTCGGGCCGTTGTTTC+TGG 0.111017 4_1:-87554637 Msa0581980:CDS
TCCATCTCTCCCACTGCTTA+TGG 0.186529 4_1:-87556205 Msa0581980:CDS
TACACACAGGATCCTGTTAT+TGG 0.238161 4_1:-87554200 Msa0581980:three_prime_UTR
CAAAACATTCTCTTAAGATT+TGG 0.238823 4_1:+87554043 None:intergenic
CGGGCCGTTGTTTCTGGTAT+TGG 0.254686 4_1:-87554631 Msa0581980:CDS
CAACCTCGATATACAGCTTA+TGG 0.287602 4_1:-87554457 Msa0581980:CDS
TTAAACCTTAAAACCAAATC+TGG 0.303799 4_1:+87554263 None:intergenic
TCGATGCAGGTTCTTAATTC+AGG 0.309680 4_1:-87554659 Msa0581980:CDS
GGTTCTTAATTCAGGTGGTT+CGG 0.310782 4_1:-87554651 Msa0581980:CDS
TTTCATCTCTAATCCAGATT+TGG 0.311746 4_1:-87554276 Msa0581980:three_prime_UTR
GATTCTGATCCACTTGGATT+AGG 0.315458 4_1:-87554553 Msa0581980:CDS
GGGTTTGGTTGTGGATGAAA+TGG 0.321666 4_1:+87555110 None:intergenic
CTCTTGAGTCTCATTTCCTT+TGG 0.330899 4_1:+87554154 None:intergenic
CCACCTCTCTAGAAGGGGTT+TGG 0.330917 4_1:+87555095 None:intergenic
GTTCTTAATTCAGGTGGTTC+GGG 0.333795 4_1:-87554650 Msa0581980:CDS
CCATCTCTCCCACTGCTTAT+GGG 0.338294 4_1:-87556204 Msa0581980:intron
CTCTAGAAGGGGTTTGGTTG+TGG 0.342961 4_1:+87555101 None:intergenic
GTTCGTACAACAAACCAAAA+TGG 0.347218 4_1:+87554077 None:intergenic
TGTTCCTCTGAATAAATTCC+AGG 0.349073 4_1:+87555433 None:intergenic
CTCCTCTGAACAAGTATAAC+AGG 0.362982 4_1:-87554232 Msa0581980:three_prime_UTR
GTTGGTCGTGCATCCCATTC+TGG 0.363613 4_1:-87555337 Msa0581980:intron
TTGAAGTTTGCAATCTTGTA+AGG 0.367147 4_1:+87556443 None:intergenic
TGTGCAGTGGTGGATATAAT+AGG 0.370705 4_1:-87554358 Msa0581980:three_prime_UTR
GTGGTAAGCGCTCGAGGCTC+CGG 0.373825 4_1:+87555036 None:intergenic
GGTGGATATAATAGGAAGTT+AGG 0.374612 4_1:-87554350 Msa0581980:three_prime_UTR
AGCTCAAGGTGATGCTGATT+TGG 0.377196 4_1:-87554315 Msa0581980:three_prime_UTR
GAATTCTCCACCTCTCTAGA+AGG 0.384447 4_1:+87555088 None:intergenic
ATTGATCAATTCTTGAAAGA+TGG 0.389055 4_1:-87554724 Msa0581980:CDS
CGCAGGTTTCCCCATGTACC+TGG 0.393784 4_1:-87555451 Msa0581980:intron
TAATCGGTGTAGCCAATAAC+AGG 0.415841 4_1:+87554188 None:intergenic
TGGTTTGTTGTACGAACAAT+TGG 0.420686 4_1:-87554071 Msa0581980:three_prime_UTR
GAACCTAAGAAAAGCTTATG+AGG 0.420831 4_1:-87554390 Msa0581980:three_prime_UTR
GATGCTGATTTGGTATCATT+TGG 0.426648 4_1:-87554305 Msa0581980:three_prime_UTR
CTGAATAAATTCCAGGTACA+TGG 0.427423 4_1:+87555440 None:intergenic
TGAAATCGAATGATTATTGA+AGG 0.436371 4_1:+87556613 None:intergenic
AATGATCGAACAGACGAGTA+TGG 0.441181 4_1:-87554697 Msa0581980:CDS
TATTCAGCAAGTGCTGCGTT+TGG 0.442605 4_1:+87556277 None:intergenic
AACGCTCTGCATCTCGTCAT+AGG 0.445839 4_1:+87556310 None:intergenic
TTTGGTGAGGAAAGGATAAT+CGG 0.449437 4_1:+87554172 None:intergenic
ATTGGAGTGCAGGGTGGTGT+TGG 0.449570 4_1:-87556335 Msa0581980:intron
GCTATTAATGCGATTCGAGC+AGG 0.471945 4_1:-87554973 Msa0581980:intron
TTGATCAATTCTTGAAAGAT+GGG 0.478672 4_1:-87554723 Msa0581980:CDS
CTCAAGCCAGATGGTTCCTA+TGG 0.479424 4_1:-87555066 Msa0581980:CDS
ACAAAAGATAATGCTGCCTT+TGG 0.485408 4_1:+87555372 None:intergenic
AGGTTGAGTAACATGAAGAT+AGG 0.492670 4_1:+87554474 None:intergenic
GAAACTGAGGTTACCAGAAT+GGG 0.497238 4_1:+87555324 None:intergenic
AGGCTCCGGGTATGCGCCAT+AGG 0.499161 4_1:+87555050 None:intergenic
GGTTTGGTTGTGGATGAAAT+GGG 0.499316 4_1:+87555111 None:intergenic
TTGATGGAGTCGAAATTCAC+GGG 0.503324 4_1:-87554762 Msa0581980:CDS
GCATCATTGTGATCAATTGC+TGG 0.504056 4_1:+87554580 None:intergenic
TGAAACTGAGGTTACCAGAA+TGG 0.512235 4_1:+87555323 None:intergenic
TTTGATGGAGTCGAAATTCA+CGG 0.512397 4_1:-87554763 Msa0581980:CDS
AGGAGAAAAGAGGGTGGTGC+TGG 0.515328 4_1:+87556463 None:intergenic
TGGTAAGCGCTCGAGGCTCC+GGG 0.517375 4_1:+87555037 None:intergenic
CCCATAAGCAGTGGGAGAGA+TGG 0.517721 4_1:+87556204 None:intergenic
TCATTTCCTTTGGTGAGGAA+AGG 0.518047 4_1:+87554164 None:intergenic
TCTGGAAGTTGATCCCAATC+AGG 0.520097 4_1:-87556573 Msa0581980:five_prime_UTR
CAATCTTGTAAGGAGAAAAG+AGG 0.521873 4_1:+87556453 None:intergenic
GAATCGCATTAATAGCTGAT+TGG 0.525902 4_1:+87554980 None:intergenic
GCTATTGATTCTGATCCACT+TGG 0.530394 4_1:-87554559 Msa0581980:CDS
TATGCGCCATAGGAACCATC+TGG 0.531081 4_1:+87555060 None:intergenic
GTCGAAATTCACGGGGCACA+CGG 0.533112 4_1:-87554754 Msa0581980:CDS
GAGTCTCATTTCCTTTGGTG+AGG 0.533954 4_1:+87554159 None:intergenic
ACGAGATGCAGAGCGTTGAA+TGG 0.535942 4_1:-87556304 Msa0581980:CDS
GAAATCGAATGATTATTGAA+GGG 0.540825 4_1:+87556614 None:intergenic
TTCCTGTTATACTTGTTCAG+AGG 0.544474 4_1:+87554230 None:intergenic
AATTCTCCACCTCTCTAGAA+GGG 0.550426 4_1:+87555089 None:intergenic
TCGAAATTCACGGGGCACAC+GGG 0.550944 4_1:-87554753 Msa0581980:CDS
TGAATAAATTCCAGGTACAT+GGG 0.553359 4_1:+87555441 None:intergenic
TGGTTCCTATGGCGCATACC+CGG 0.554696 4_1:-87555055 Msa0581980:CDS
GTATGACAAGGAAGAAGAAG+AGG 0.555686 4_1:-87554426 Msa0581980:CDS
GAAATGGGTGCAGCACCACC+AGG 0.555752 4_1:+87555126 None:intergenic
CAGAGGAACAAGTGGAGGCA+TGG 0.561445 4_1:-87555420 Msa0581980:CDS
GGTGGATTTCTCATCGGTGA+AGG 0.575119 4_1:-87556232 Msa0581980:CDS
AAATGTAATCTTATATATCG+TGG 0.578530 4_1:-87554015 Msa0581980:three_prime_UTR
GATCACTTGAAAATCGATGC+AGG 0.580483 4_1:-87554672 Msa0581980:CDS
ATTATATACCCATAAGCAGT+GGG 0.587040 4_1:+87556196 None:intergenic
ATGCAGGTTCTTAATTCAGG+TGG 0.588415 4_1:-87554656 Msa0581980:CDS
TCAGAAGTGGTAAGCGCTCG+AGG 0.590830 4_1:+87555030 None:intergenic
ATATAATAGGAAGTTAGGAA+TGG 0.592051 4_1:-87554345 Msa0581980:three_prime_UTR
GGAATGGAAGCTGTAGCTCA+AGG 0.592444 4_1:-87554329 Msa0581980:three_prime_UTR
CCAAACCCCTTCTAGAGAGG+TGG 0.592960 4_1:-87555095 Msa0581980:CDS
AGTTCAACAACACAAGTACA+AGG 0.594788 4_1:-87556491 Msa0581980:CDS
TATTTCTGGTATCTCAGAAG+TGG 0.602504 4_1:+87555017 None:intergenic
TACGCTCAAAGATCAACTCC+AGG 0.602812 4_1:-87556253 Msa0581980:CDS
GATTATCCTTTCCTCACCAA+AGG 0.604147 4_1:-87554170 Msa0581980:three_prime_UTR
GGAACATTCATGTGCAGTGG+TGG 0.609749 4_1:-87554368 Msa0581980:three_prime_UTR
TTATTCAGAGGAACAAGTGG+AGG 0.610128 4_1:-87555425 Msa0581980:CDS
AATTTATTCAGAGGAACAAG+TGG 0.610952 4_1:-87555428 Msa0581980:CDS
TTTCCATAAGCTGTATATCG+AGG 0.611276 4_1:+87554454 None:intergenic
ATAAGCAGTGGGAGAGATGG+AGG 0.611650 4_1:+87556207 None:intergenic
AAGAAGAGGCTCATTTGATG+AGG 0.613862 4_1:-87554412 Msa0581980:exon
CAACCAAACCCCTTCTAGAG+AGG 0.615926 4_1:-87555098 Msa0581980:CDS
ATTCTCCACCTCTCTAGAAG+GGG 0.617495 4_1:+87555090 None:intergenic
AACCTAAGAAAAGCTTATGA+GGG 0.618035 4_1:-87554389 Msa0581980:three_prime_UTR
TGGAGAATTCTCAAGCCAGA+TGG 0.619198 4_1:-87555075 Msa0581980:CDS
CAAAATATATATATTAACAA+TGG 0.622360 4_1:-87556531 Msa0581980:exon
GGTATTGGAGCTGAAAGAGT+TGG 0.624730 4_1:-87554616 Msa0581980:CDS
GAATAAATTCCAGGTACATG+GGG 0.626230 4_1:+87555442 None:intergenic
ACTCCAGGTGGATTTCTCAT+CGG 0.631444 4_1:-87556238 Msa0581980:CDS
AATTATATACCCATAAGCAG+TGG 0.632930 4_1:+87556195 None:intergenic
ACTGCTAAGCCTAATCCAAG+TGG 0.645958 4_1:+87554544 None:intergenic
AGCTCCAATACCAGAAACAA+CGG 0.648152 4_1:+87554627 None:intergenic
TGTGAAGCAGTGTATCAGCC+TGG 0.649524 4_1:-87555144 Msa0581980:intron
GTGGATGCTGCTCATGCCAA+AGG 0.652152 4_1:-87555388 Msa0581980:CDS
GATCGAACAGACGAGTATGG+TGG 0.652328 4_1:-87554694 Msa0581980:CDS
TGAGATACCAGAAATAGTCA+AGG 0.658712 4_1:-87555010 Msa0581980:CDS
TGTACCTGGAATTTATTCAG+AGG 0.660053 4_1:-87555437 Msa0581980:CDS
TCACCGATGAGAAATCCACC+TGG 0.665351 4_1:+87556235 None:intergenic
AATCTTGTAAGGAGAAAAGA+GGG 0.669109 4_1:+87556454 None:intergenic
GGAGGCATGGAGAAATGTCG+TGG 0.680628 4_1:-87555407 Msa0581980:CDS
ATGGAAAACTGAGTATGACA+AGG 0.683603 4_1:-87554438 Msa0581980:CDS
GCTCAAAGATCAACTCCAGG+TGG 0.683876 4_1:-87556250 Msa0581980:CDS
GAGGGAACATTCATGTGCAG+TGG 0.698747 4_1:-87554371 Msa0581980:three_prime_UTR
TGATGGAGTCGAAATTCACG+GGG 0.717446 4_1:-87554761 Msa0581980:CDS
GAAGCAGTGTATCAGCCTGG+TGG 0.735693 4_1:-87555141 Msa0581980:intron
CTTGTAAGGAGAAAAGAGGG+TGG 0.760384 4_1:+87556457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATATATATATTTTGTGA+TGG + chr4_1:87553999-87554018 None:intergenic 10.0%
!! CAAAATATATATATTAACAA+TGG - chr4_1:87554002-87554021 Msa0581980:three_prime_UTR 10.0%
!!! AAATTTGGATTATTTTTTTA+AGG + chr4_1:87554509-87554528 None:intergenic 10.0%
!!! TGTAATATTTTCATATATTT+AGG + chr4_1:87555031-87555050 None:intergenic 10.0%
!! TATATGAAAATATTACATTA+TGG - chr4_1:87555034-87555053 Msa0581980:CDS 10.0%
!!! TTTTTTTTTTTTTACCATTT+TGG - chr4_1:87556442-87556461 Msa0581980:CDS 10.0%
!! AAACAGTTATAAAGTAATAA+AGG - chr4_1:87554566-87554585 Msa0581980:CDS 15.0%
!!! ATAAAGTAATAAAGGTATAT+TGG - chr4_1:87554574-87554593 Msa0581980:CDS 15.0%
!! AATATAAAAGAAATATAGGA+CGG - chr4_1:87554641-87554660 Msa0581980:CDS 15.0%
!! AGTACATAAAACTAAATAAA+CGG - chr4_1:87554803-87554822 Msa0581980:intron 15.0%
!!! TATTTTCATATATTTAGGAT+TGG + chr4_1:87555026-87555045 None:intergenic 15.0%
!!! AATACTACATTTTTAGATTC+TGG - chr4_1:87553942-87553961 Msa0581980:three_prime_UTR 20.0%
!!! TTTTTTTTCTCTGTTATTGT+GGG - chr4_1:87554149-87554168 Msa0581980:three_prime_UTR 20.0%
!! AAATAATATTATGTTGATGC+TGG - chr4_1:87554462-87554481 Msa0581980:CDS 20.0%
!! ATACATCTAACAATCAAATT+TGG + chr4_1:87554524-87554543 None:intergenic 20.0%
!!! TTTATCCTTTGAAATTTGTA+AGG + chr4_1:87554619-87554638 None:intergenic 20.0%
!! ACATTATGGTATTGTAATAT+AGG - chr4_1:87555048-87555067 Msa0581980:CDS 20.0%
!! CATTATGGTATTGTAATATA+GGG - chr4_1:87555049-87555068 Msa0581980:CDS 20.0%
!! AAATGTAATCTTATATATCG+TGG - chr4_1:87556518-87556537 Msa0581980:exon 20.0%
! GAAATCGAATGATTATTGAA+GGG + chr4_1:87553922-87553941 None:intergenic 25.0%
! TGAAATCGAATGATTATTGA+AGG + chr4_1:87553923-87553942 None:intergenic 25.0%
!!! GTTTTTTTTCTCTGTTATTG+TGG - chr4_1:87554148-87554167 Msa0581980:three_prime_UTR 25.0%
! AAAGAAAAAGCTTGAAAAAG+AGG + chr4_1:87554364-87554383 None:intergenic 25.0%
! GACATCAATGAATATAAGTT+AGG + chr4_1:87554415-87554434 None:intergenic 25.0%
! TTTGATTGTTAGATGTATGT+TGG - chr4_1:87554526-87554545 Msa0581980:CDS 25.0%
! TGTATGTTGGTATTTACTAT+TGG - chr4_1:87554539-87554558 Msa0581980:CDS 25.0%
! ATTGTCCTTACAAATTTCAA+AGG - chr4_1:87554611-87554630 Msa0581980:CDS 25.0%
! TTGTAATATAGGGAAATGTT+TGG - chr4_1:87555059-87555078 Msa0581980:CDS 25.0%
! TTCACAATTAAATCATTGAG+TGG + chr4_1:87555375-87555394 None:intergenic 25.0%
! TATTTGTTTACATCTCTTCA+AGG - chr4_1:87555588-87555607 Msa0581980:intron 25.0%
! CTCACAAACATAAAATAGTA+AGG + chr4_1:87555628-87555647 None:intergenic 25.0%
! ATTGATCAATTCTTGAAAGA+TGG - chr4_1:87555809-87555828 Msa0581980:intron 25.0%
! TTGATCAATTCTTGAAAGAT+GGG - chr4_1:87555810-87555829 Msa0581980:intron 25.0%
! ATATAATAGGAAGTTAGGAA+TGG - chr4_1:87556188-87556207 Msa0581980:intron 25.0%
! TTAAACCTTAAAACCAAATC+TGG + chr4_1:87556273-87556292 None:intergenic 25.0%
! CAAAACATTCTCTTAAGATT+TGG + chr4_1:87556493-87556512 None:intergenic 25.0%
! ATGAATTTTCTCTCCTGATT+GGG + chr4_1:87553976-87553995 None:intergenic 30.0%
AATCTTGTAAGGAGAAAAGA+GGG + chr4_1:87554082-87554101 None:intergenic 30.0%
TTGAAGTTTGCAATCTTGTA+AGG + chr4_1:87554093-87554112 None:intergenic 30.0%
! CAAACTTCAACTTTTCTCAT+AGG - chr4_1:87554103-87554122 Msa0581980:three_prime_UTR 30.0%
ATTATATACCCATAAGCAGT+GGG + chr4_1:87554340-87554359 None:intergenic 30.0%
AATTATATACCCATAAGCAG+TGG + chr4_1:87554341-87554360 None:intergenic 30.0%
GAATAGTACTAAAACCATGT+AGG + chr4_1:87554842-87554861 None:intergenic 30.0%
TGAATAAATTCCAGGTACAT+GGG + chr4_1:87555095-87555114 None:intergenic 30.0%
AATTTATTCAGAGGAACAAG+TGG - chr4_1:87555105-87555124 Msa0581980:CDS 30.0%
! GCATTATCTTTTGTCAGTTA+TGG - chr4_1:87555170-87555189 Msa0581980:intron 30.0%
! GTTTTCAATGCAGTTCATTA+AGG + chr4_1:87555262-87555281 None:intergenic 30.0%
AACCTAAGAAAAGCTTATGA+GGG - chr4_1:87556144-87556163 Msa0581980:intron 30.0%
TTTCATCTCTAATCCAGATT+TGG - chr4_1:87556257-87556276 Msa0581980:CDS 30.0%
!!! CTAATCCAGATTTGGTTTTA+AGG - chr4_1:87556265-87556284 Msa0581980:CDS 30.0%
GAAGGAAAAACAACAATCAA+AGG + chr4_1:87556416-87556435 None:intergenic 30.0%
! GATGAATTTTCTCTCCTGAT+TGG + chr4_1:87553977-87553996 None:intergenic 35.0%
AGTTCAACAACACAAGTACA+AGG - chr4_1:87554042-87554061 Msa0581980:three_prime_UTR 35.0%
CAATCTTGTAAGGAGAAAAG+AGG + chr4_1:87554083-87554102 None:intergenic 35.0%
ACTATGATCTGATGTGGTTA+TGG - chr4_1:87554174-87554193 Msa0581980:three_prime_UTR 35.0%
ATCTGATGTGGTTATGGTAT+TGG - chr4_1:87554180-87554199 Msa0581980:three_prime_UTR 35.0%
! CATGCATCTGATTTTGTCAA+TGG - chr4_1:87554706-87554725 Msa0581980:CDS 35.0%
AGTTGCGACAAATGAATCAA+GGG + chr4_1:87554739-87554758 None:intergenic 35.0%
AAGTTGCGACAAATGAATCA+AGG + chr4_1:87554740-87554759 None:intergenic 35.0%
GTCAAACAAAAAGTCCTACA+TGG - chr4_1:87554825-87554844 Msa0581980:intron 35.0%
TTAAACACAATCAGCCTCAT+TGG + chr4_1:87554877-87554896 None:intergenic 35.0%
ATCAACGACAAAAAGAACAC+AGG + chr4_1:87554920-87554939 None:intergenic 35.0%
GAATAAATTCCAGGTACATG+GGG + chr4_1:87555094-87555113 None:intergenic 35.0%
CTGAATAAATTCCAGGTACA+TGG + chr4_1:87555096-87555115 None:intergenic 35.0%
TGTACCTGGAATTTATTCAG+AGG - chr4_1:87555096-87555115 Msa0581980:CDS 35.0%
TGTTCCTCTGAATAAATTCC+AGG + chr4_1:87555103-87555122 None:intergenic 35.0%
ACAAAAGATAATGCTGCCTT+TGG + chr4_1:87555164-87555183 None:intergenic 35.0%
! TTTTGTCAGTTATGGCATGT+TGG - chr4_1:87555178-87555197 Msa0581980:intron 35.0%
! CATTTAGGATAGTGAAACTG+AGG + chr4_1:87555225-87555244 None:intergenic 35.0%
! GATGAACTTGTGAAGCATTT+AGG + chr4_1:87555240-87555259 None:intergenic 35.0%
!! TATTTCTGGTATCTCAGAAG+TGG + chr4_1:87555519-87555538 None:intergenic 35.0%
TGAGATACCAGAAATAGTCA+AGG - chr4_1:87555523-87555542 Msa0581980:intron 35.0%
CGATAATCCTTGACTATTTC+TGG + chr4_1:87555533-87555552 None:intergenic 35.0%
GAATCGCATTAATAGCTGAT+TGG + chr4_1:87555556-87555575 None:intergenic 35.0%
ATGAAATGAGACGTACATAC+AGG - chr4_1:87555746-87555765 Msa0581980:intron 35.0%
TTTGATGGAGTCGAAATTCA+CGG - chr4_1:87555770-87555789 Msa0581980:intron 35.0%
AACAATCTCCAAAAAGAGCT+TGG - chr4_1:87556025-87556044 Msa0581980:intron 35.0%
! GTTTTCTACCAAGCTCTTTT+TGG + chr4_1:87556036-87556055 None:intergenic 35.0%
AGGTTGAGTAACATGAAGAT+AGG + chr4_1:87556062-87556081 None:intergenic 35.0%
TTTCCATAAGCTGTATATCG+AGG + chr4_1:87556082-87556101 None:intergenic 35.0%
ATGGAAAACTGAGTATGACA+AGG - chr4_1:87556095-87556114 Msa0581980:intron 35.0%
GAACCTAAGAAAAGCTTATG+AGG - chr4_1:87556143-87556162 Msa0581980:intron 35.0%
! TTCCCTCATAAGCTTTTCTT+AGG + chr4_1:87556149-87556168 None:intergenic 35.0%
GGTGGATATAATAGGAAGTT+AGG - chr4_1:87556183-87556202 Msa0581980:intron 35.0%
!! GATGCTGATTTGGTATCATT+TGG - chr4_1:87556228-87556247 Msa0581980:CDS 35.0%
TTCCTGTTATACTTGTTCAG+AGG + chr4_1:87556306-87556325 None:intergenic 35.0%
TTTGGTGAGGAAAGGATAAT+CGG + chr4_1:87556364-87556383 None:intergenic 35.0%
GTTCGTACAACAAACCAAAA+TGG + chr4_1:87556459-87556478 None:intergenic 35.0%
!! TGGTTTGTTGTACGAACAAT+TGG - chr4_1:87556462-87556481 Msa0581980:CDS 35.0%
! CATCTGATTTTGTCAATGGC+CGG - chr4_1:87554710-87554729 Msa0581980:CDS 40.0%
TTATTCAGAGGAACAAGTGG+AGG - chr4_1:87555108-87555127 Msa0581980:CDS 40.0%
GAAACTGAGGTTACCAGAAT+GGG + chr4_1:87555212-87555231 None:intergenic 40.0%
TGAAACTGAGGTTACCAGAA+TGG + chr4_1:87555213-87555232 None:intergenic 40.0%
! GGTTTGGTTGTGGATGAAAT+GGG + chr4_1:87555425-87555444 None:intergenic 40.0%
! AATTCTCCACCTCTCTAGAA+GGG + chr4_1:87555447-87555466 None:intergenic 40.0%
!! GACGTACATACAGGTTTTGA+TGG - chr4_1:87555755-87555774 Msa0581980:intron 40.0%
TTGATGGAGTCGAAATTCAC+GGG - chr4_1:87555771-87555790 Msa0581980:intron 40.0%
AATGATCGAACAGACGAGTA+TGG - chr4_1:87555836-87555855 Msa0581980:intron 40.0%
GATCACTTGAAAATCGATGC+AGG - chr4_1:87555861-87555880 Msa0581980:intron 40.0%
TCGATGCAGGTTCTTAATTC+AGG - chr4_1:87555874-87555893 Msa0581980:intron 40.0%
ATGCAGGTTCTTAATTCAGG+TGG - chr4_1:87555877-87555896 Msa0581980:intron 40.0%
GGTTCTTAATTCAGGTGGTT+CGG - chr4_1:87555882-87555901 Msa0581980:intron 40.0%
GTTCTTAATTCAGGTGGTTC+GGG - chr4_1:87555883-87555902 Msa0581980:intron 40.0%
AGCTCCAATACCAGAAACAA+CGG + chr4_1:87555909-87555928 None:intergenic 40.0%
GCATCATTGTGATCAATTGC+TGG + chr4_1:87555956-87555975 None:intergenic 40.0%
!! GCTATTGATTCTGATCCACT+TGG - chr4_1:87555974-87555993 Msa0581980:intron 40.0%
GATTCTGATCCACTTGGATT+AGG - chr4_1:87555980-87555999 Msa0581980:intron 40.0%
CAACCTCGATATACAGCTTA+TGG - chr4_1:87556076-87556095 Msa0581980:intron 40.0%
GTATGACAAGGAAGAAGAAG+AGG - chr4_1:87556107-87556126 Msa0581980:intron 40.0%
AAGAAGAGGCTCATTTGATG+AGG - chr4_1:87556121-87556140 Msa0581980:intron 40.0%
!! TGTGCAGTGGTGGATATAAT+AGG - chr4_1:87556175-87556194 Msa0581980:intron 40.0%
CTCCTCTGAACAAGTATAAC+AGG - chr4_1:87556301-87556320 Msa0581980:CDS 40.0%
! CAGGAAGACATTTTACACAC+AGG - chr4_1:87556320-87556339 Msa0581980:CDS 40.0%
TACACACAGGATCCTGTTAT+TGG - chr4_1:87556333-87556352 Msa0581980:intron 40.0%
TAATCGGTGTAGCCAATAAC+AGG + chr4_1:87556348-87556367 None:intergenic 40.0%
GATTATCCTTTCCTCACCAA+AGG - chr4_1:87556363-87556382 Msa0581980:intron 40.0%
TCATTTCCTTTGGTGAGGAA+AGG + chr4_1:87556372-87556391 None:intergenic 40.0%
CTCTTGAGTCTCATTTCCTT+TGG + chr4_1:87556382-87556401 None:intergenic 40.0%
! TCTGGAAGTTGATCCCAATC+AGG - chr4_1:87553960-87553979 Msa0581980:three_prime_UTR 45.0%
CTTGTAAGGAGAAAAGAGGG+TGG + chr4_1:87554079-87554098 None:intergenic 45.0%
TGGGTGACTATGATCTGATG+TGG - chr4_1:87554168-87554187 Msa0581980:three_prime_UTR 45.0%
! TGGTTATGGTATTGGAGTGC+AGG - chr4_1:87554188-87554207 Msa0581980:three_prime_UTR 45.0%
! GGTTATGGTATTGGAGTGCA+GGG - chr4_1:87554189-87554208 Msa0581980:three_prime_UTR 45.0%
! TATTCAGCAAGTGCTGCGTT+TGG + chr4_1:87554259-87554278 None:intergenic 45.0%
TACGCTCAAAGATCAACTCC+AGG - chr4_1:87554280-87554299 Msa0581980:three_prime_UTR 45.0%
! ACTCCAGGTGGATTTCTCAT+CGG - chr4_1:87554295-87554314 Msa0581980:three_prime_UTR 45.0%
ACAAATGAATCAAGGGTGGC+CGG + chr4_1:87554732-87554751 None:intergenic 45.0%
TGCGACAAATGAATCAAGGG+TGG + chr4_1:87554736-87554755 None:intergenic 45.0%
TCTATCGCTACCAACCAATG+AGG - chr4_1:87554860-87554879 Msa0581980:intron 45.0%
ACACAATCAGCCTCATTGGT+TGG + chr4_1:87554873-87554892 None:intergenic 45.0%
TATAGGGAAATGTTTGGCGC+AGG - chr4_1:87555065-87555084 Msa0581980:CDS 45.0%
! GGGTTTGGTTGTGGATGAAA+TGG + chr4_1:87555426-87555445 None:intergenic 45.0%
! ATTCTCCACCTCTCTAGAAG+GGG + chr4_1:87555446-87555465 None:intergenic 45.0%
GAATTCTCCACCTCTCTAGA+AGG + chr4_1:87555448-87555467 None:intergenic 45.0%
TGGAGAATTCTCAAGCCAGA+TGG - chr4_1:87555458-87555477 Msa0581980:intron 45.0%
GCTATTAATGCGATTCGAGC+AGG - chr4_1:87555560-87555579 Msa0581980:intron 45.0%
ATACTCGTGTTGCATCTGCA+CGG - chr4_1:87555672-87555691 Msa0581980:intron 45.0%
TGATGGAGTCGAAATTCACG+GGG - chr4_1:87555772-87555791 Msa0581980:intron 45.0%
! GGTATTGGAGCTGAAAGAGT+TGG - chr4_1:87555917-87555936 Msa0581980:intron 45.0%
ACTGCTAAGCCTAATCCAAG+TGG + chr4_1:87555992-87556011 None:intergenic 45.0%
! AGCTCAAGGTGATGCTGATT+TGG - chr4_1:87556218-87556237 Msa0581980:CDS 45.0%
GAGTCTCATTTCCTTTGGTG+AGG + chr4_1:87556377-87556396 None:intergenic 45.0%
!! AAAAAATATAAAAGAAATAT+AGG - chr4_1:87554637-87554656 Msa0581980:CDS 5.0%
!! AAAAAAAAAAAAAAAAAAGA+AGG + chr4_1:87556434-87556453 None:intergenic 5.0%
! TATGGTATTGGAGTGCAGGG+TGG - chr4_1:87554192-87554211 Msa0581980:three_prime_UTR 50.0%
AACGCTCTGCATCTCGTCAT+AGG + chr4_1:87554226-87554245 None:intergenic 50.0%
!! ACGAGATGCAGAGCGTTGAA+TGG - chr4_1:87554229-87554248 Msa0581980:three_prime_UTR 50.0%
GCTCAAAGATCAACTCCAGG+TGG - chr4_1:87554283-87554302 Msa0581980:three_prime_UTR 50.0%
TCACCGATGAGAAATCCACC+TGG + chr4_1:87554301-87554320 None:intergenic 50.0%
! GGTGGATTTCTCATCGGTGA+AGG - chr4_1:87554301-87554320 Msa0581980:three_prime_UTR 50.0%
ATAAGCAGTGGGAGAGATGG+AGG + chr4_1:87554329-87554348 None:intergenic 50.0%
TCCATCTCTCCCACTGCTTA+TGG - chr4_1:87554328-87554347 Msa0581980:three_prime_UTR 50.0%
CCATCTCTCCCACTGCTTAT+GGG - chr4_1:87554329-87554348 Msa0581980:three_prime_UTR 50.0%
TGTGAAGCAGTGTATCAGCC+TGG - chr4_1:87555389-87555408 Msa0581980:CDS 50.0%
! CTCTAGAAGGGGTTTGGTTG+TGG + chr4_1:87555435-87555454 None:intergenic 50.0%
! CAACCAAACCCCTTCTAGAG+AGG - chr4_1:87555435-87555454 Msa0581980:CDS 50.0%
CTCAAGCCAGATGGTTCCTA+TGG - chr4_1:87555467-87555486 Msa0581980:intron 50.0%
TATGCGCCATAGGAACCATC+TGG + chr4_1:87555476-87555495 None:intergenic 50.0%
GTGCAGATGCAACACGAGTA+TGG + chr4_1:87555673-87555692 None:intergenic 50.0%
TACTCGTGTTGCATCTGCAC+GGG - chr4_1:87555673-87555692 Msa0581980:intron 50.0%
TGTTGCATCTGCACGGGTAT+GGG - chr4_1:87555679-87555698 Msa0581980:intron 50.0%
GATCGAACAGACGAGTATGG+TGG - chr4_1:87555839-87555858 Msa0581980:intron 50.0%
GAGGGAACATTCATGTGCAG+TGG - chr4_1:87556162-87556181 Msa0581980:intron 50.0%
GGAACATTCATGTGCAGTGG+TGG - chr4_1:87556165-87556184 Msa0581980:intron 50.0%
GGAATGGAAGCTGTAGCTCA+AGG - chr4_1:87556204-87556223 Msa0581980:intron 50.0%
AGGAGAAAAGAGGGTGGTGC+TGG + chr4_1:87554073-87554092 None:intergenic 55.0%
ATTGGAGTGCAGGGTGGTGT+TGG - chr4_1:87554198-87554217 Msa0581980:three_prime_UTR 55.0%
CCCATAAGCAGTGGGAGAGA+TGG + chr4_1:87554332-87554351 None:intergenic 55.0%
CAGAGGAACAAGTGGAGGCA+TGG - chr4_1:87555113-87555132 Msa0581980:CDS 55.0%
! GGAGGCATGGAGAAATGTCG+TGG - chr4_1:87555126-87555145 Msa0581980:CDS 55.0%
GTGGATGCTGCTCATGCCAA+AGG - chr4_1:87555145-87555164 Msa0581980:intron 55.0%
GTTGGTCGTGCATCCCATTC+TGG - chr4_1:87555196-87555215 Msa0581980:intron 55.0%
GAAGCAGTGTATCAGCCTGG+TGG - chr4_1:87555392-87555411 Msa0581980:CDS 55.0%
! CCACCTCTCTAGAAGGGGTT+TGG + chr4_1:87555441-87555460 None:intergenic 55.0%
! CCAAACCCCTTCTAGAGAGG+TGG - chr4_1:87555438-87555457 Msa0581980:CDS 55.0%
TGGTTCCTATGGCGCATACC+CGG - chr4_1:87555478-87555497 Msa0581980:intron 55.0%
TCAGAAGTGGTAAGCGCTCG+AGG + chr4_1:87555506-87555525 None:intergenic 55.0%
GTGTTGCATCTGCACGGGTA+TGG - chr4_1:87555678-87555697 Msa0581980:intron 55.0%
GTCGAAATTCACGGGGCACA+CGG - chr4_1:87555779-87555798 Msa0581980:intron 55.0%
TCGAAATTCACGGGGCACAC+GGG - chr4_1:87555780-87555799 Msa0581980:intron 55.0%
!! CGGGCCGTTGTTTCTGGTAT+TGG - chr4_1:87555902-87555921 Msa0581980:intron 55.0%
CGCAGGTTTCCCCATGTACC+TGG - chr4_1:87555082-87555101 Msa0581980:CDS 60.0%
GAAATGGGTGCAGCACCACC+AGG + chr4_1:87555410-87555429 None:intergenic 60.0%
! GTGGTTCGGGCCGTTGTTTC+TGG - chr4_1:87555896-87555915 Msa0581980:intron 60.0%
AGGCTCCGGGTATGCGCCAT+AGG + chr4_1:87555486-87555505 None:intergenic 65.0%
TGGTAAGCGCTCGAGGCTCC+GGG + chr4_1:87555499-87555518 None:intergenic 65.0%
GTGGTAAGCGCTCGAGGCTC+CGG + chr4_1:87555500-87555519 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 87553912 87556643 87553912 ID=Msa0581980;Name=Msa0581980
chr4_1 mRNA 87553912 87556643 87553912 ID=Msa0581980-mRNA-1;Parent=Msa0581980;Name=Msa0581980-mRNA-1;_AED=0.04;_eAED=0.04;_QI=109|1|1|1|1|1|5|505|309
chr4_1 exon 87553912 87554787 87553912 ID=Msa0581980-mRNA-1:exon:32582;Parent=Msa0581980-mRNA-1
chr4_1 exon 87554974 87555156 87554974 ID=Msa0581980-mRNA-1:exon:32581;Parent=Msa0581980-mRNA-1
chr4_1 exon 87555338 87555468 87555338 ID=Msa0581980-mRNA-1:exon:32580;Parent=Msa0581980-mRNA-1
chr4_1 exon 87556205 87556345 87556205 ID=Msa0581980-mRNA-1:exon:32579;Parent=Msa0581980-mRNA-1
chr4_1 exon 87556431 87556643 87556431 ID=Msa0581980-mRNA-1:exon:32578;Parent=Msa0581980-mRNA-1
chr4_1 five_prime_UTR 87556535 87556643 87556535 ID=Msa0581980-mRNA-1:five_prime_utr;Parent=Msa0581980-mRNA-1
chr4_1 CDS 87556431 87556534 87556431 ID=Msa0581980-mRNA-1:cds;Parent=Msa0581980-mRNA-1
chr4_1 CDS 87556205 87556345 87556205 ID=Msa0581980-mRNA-1:cds;Parent=Msa0581980-mRNA-1
chr4_1 CDS 87555338 87555468 87555338 ID=Msa0581980-mRNA-1:cds;Parent=Msa0581980-mRNA-1
chr4_1 CDS 87554974 87555156 87554974 ID=Msa0581980-mRNA-1:cds;Parent=Msa0581980-mRNA-1
chr4_1 CDS 87554417 87554787 87554417 ID=Msa0581980-mRNA-1:cds;Parent=Msa0581980-mRNA-1
chr4_1 three_prime_UTR 87553912 87554416 87553912 ID=Msa0581980-mRNA-1:three_prime_utr;Parent=Msa0581980-mRNA-1
Gene Sequence

>Msa0581980

ATGGCAGAGTACAATAATTCAAGTTCAACAACACAAGTACAAGGAGACACCAGCACCACCCTCTTTTCTCCTTACAAGATTGCAAACTTCAACTTTTCTCATAGGGTGGTGTTGGCACCTATGACGAGATGCAGAGCGTTGAATGGTATTCCAAACGCAGCACTTGCTGAATACTACGCTCAAAGATCAACTCCAGGTGGATTTCTCATCGGTGAAGGAACCTCCATCTCTCCCACTGCTTATGGGTTTCCCCATGTACCTGGAATTTATTCAGAGGAACAAGTGGAGGCATGGAGAAATGTCGTGGATGCTGCTCATGCCAAAGGCAGCATTATCTTTTGTCAGTTATGGCATGTTGGTCGTGCATCCCATTCTGTGTATCAGCCTGGTGGTGCTGCACCCATTTCATCCACAACCAAACCCCTTCTAGAGAGGTGGAGAATTCTCAAGCCAGATGGTTCCTATGGCGCATACCCGGAGCCTCGAGCGCTTACCACTTCTGAGATACCAGAAATAGTCAAGGATTATCGCCAATCAGCTATTAATGCGATTCGAGCAGGTTTTGATGGAGTCGAAATTCACGGGGCACACGGGTATCTGATTGATCAATTCTTGAAAGATGGGATCAATGATCGAACAGACGAGTATGGTGGATCACTTGAAAATCGATGCAGGTTCTTAATTCAGGTGGTTCGGGCCGTTGTTTCTGGTATTGGAGCTGAAAGAGTTGGTGTCAGAATTTCACCAGCAATTGATCACAATGATGCTATTGATTCTGATCCACTTGGATTAGGCTTAGCAGTGATTGAGAGACTAAACAATCTCCAAAAAGAGCTTGGTAGAAAACTCACCTATCTTCATGTTACTCAACCTCGATATACAGCTTATGGAAAACTGAGTATGACAAGGAAGAAGAAGAGGCTCATTTGA
Protein sequence

>Msa0581980

MAEYNNSSSTTQVQGDTSTTLFSPYKIANFNFSHRVVLAPMTRCRALNGIPNAALAEYYAQRSTPGGFLIGEGTSISPTAYGFPHVPGIYSEEQVEAWRNVVDAAHAKGSIIFCQLWHVGRASHSVYQPGGAAPISSTTKPLLERWRILKPDGSYGAYPEPRALTTSEIPEIVKDYRQSAINAIRAGFDGVEIHGAHGYLIDQFLKDGINDRTDEYGGSLENRCRFLIQVVRAVVSGIGAERVGVRISPAIDHNDAIDSDPLGLGLAVIERLNNLQKELGRKLTYLHVTQPRYTAYGKLSMTRKKKRLI*