AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1266320

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266320 MtrunA17_Chr4g0070561 95.341 279 11 1 1 279 1 277 0.0 551
Msa1266320 MtrunA17_Chr5g0394771 56.420 257 104 1 17 273 36 284 6.06e-105 311
Msa1266320 MtrunA17_Chr5g0394891 55.939 261 113 1 13 273 4 262 9.79e-104 308
Msa1266320 MtrunA17_Chr5g0394751 56.126 253 103 1 21 273 8 252 4.19e-103 306
Msa1266320 MtrunA17_Chr3g0144001 56.274 263 107 2 14 275 3 258 3.34e-101 301
Msa1266320 MtrunA17_Chr5g0394861 56.641 256 105 3 21 273 12 264 1.96e-99 296
Msa1266320 MtrunA17_Chr5g0394811 52.083 288 129 4 21 304 121 403 1.41e-98 298
Msa1266320 MtrunA17_Chr5g0394811 42.949 156 71 3 27 164 1 156 1.84e-34 130
Msa1266320 MtrunA17_Chr5g0395841 50.871 287 134 3 21 304 14 296 5.53e-97 290
Msa1266320 MtrunA17_Chr5g0394841 50.865 289 133 4 21 304 12 296 4.91e-96 288
Msa1266320 MtrunA17_Chr5g0394791 50.595 168 73 3 21 187 11 169 2.65e-48 160
Msa1266320 MtrunA17_Chr5g0394801 59.091 88 36 0 186 273 26 113 4.43e-32 119
Msa1266320 MtrunA17_Chr3g0130471 65.116 43 15 0 205 247 1 43 4.25e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1266320 AT2G06050.2 74.906 267 66 1 11 276 2 268 1.84e-151 430
Msa1266320 AT2G06050.1 74.906 267 66 1 11 276 2 268 1.84e-151 430
Msa1266320 AT2G06050.3 74.906 267 66 1 11 276 2 268 1.84e-151 430
Msa1266320 AT1G76690.1 54.023 261 118 1 14 274 7 265 3.81e-101 301
Msa1266320 AT1G76680.1 55.000 260 115 1 14 273 5 262 2.67e-100 299
Msa1266320 AT1G17990.1 52.364 275 120 4 18 292 6 269 8.00e-100 294
Msa1266320 AT1G18020.1 52.364 275 120 4 18 292 6 269 8.00e-100 294
Msa1266320 AT1G09400.1 53.600 250 108 1 27 276 1 242 1.95e-97 290
Msa1266320 AT1G76680.2 50.000 286 116 2 14 274 5 288 1.06e-94 286
Msa1266320 AT1G17990.2 49.818 275 119 5 18 292 6 261 1.52e-91 273
Msa1266320 AT1G09400.2 54.974 191 78 1 86 276 1 183 2.66e-73 227

Find 119 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATAATCCTTGACTATTTC+TGG 0.074699 8_2:-71110780 None:intergenic
GTGGTTCGGGCCGTTGTTTC+TGG 0.111017 8_2:+71111146 Msa1266320:CDS
TCCATCTCTCCCACTGCTTA+TGG 0.186529 8_2:+71109556 Msa1266320:CDS
TACACACAGGATCCTGTTAT+TGG 0.238161 8_2:+71111583 Msa1266320:three_prime_UTR
CAAAACATTCTCTTAAGATT+TGG 0.238823 8_2:-71111739 None:intergenic
CGGGCCGTTGTTTCTGGTAT+TGG 0.254686 8_2:+71111152 Msa1266320:CDS
CAACCTCGATATACAGCTTA+TGG 0.287602 8_2:+71111325 Msa1266320:CDS
TTAAACCTTAAAACCAAATC+TGG 0.303799 8_2:-71111520 None:intergenic
TCGATGCAGGTTCTTAATTC+AGG 0.309680 8_2:+71111124 Msa1266320:CDS
GGTTCTTAATTCAGGTGGTT+CGG 0.310782 8_2:+71111132 Msa1266320:CDS
TTTCATCTCTAATCCAGATT+TGG 0.311746 8_2:+71111507 Msa1266320:three_prime_UTR
GATTCTGATCCACTTGGATT+AGG 0.315458 8_2:+71111230 Msa1266320:CDS
GGGTTTGGTTGTGGATGAAA+TGG 0.321666 8_2:-71110673 None:intergenic
CTCTTGAGTCTCATTTCCTT+TGG 0.330899 8_2:-71111629 None:intergenic
CCACCTCTCTAGAAGGGGTT+TGG 0.330917 8_2:-71110688 None:intergenic
GTTCTTAATTCAGGTGGTTC+GGG 0.333795 8_2:+71111133 Msa1266320:CDS
CCATCTCTCCCACTGCTTAT+GGG 0.338294 8_2:+71109557 Msa1266320:CDS
CTCTAGAAGGGGTTTGGTTG+TGG 0.342961 8_2:-71110682 None:intergenic
TGTTCCTCTGAATAAATTCC+AGG 0.349073 8_2:-71110350 None:intergenic
CTCCTCTGAACAAGTATAAC+AGG 0.362982 8_2:+71111551 Msa1266320:three_prime_UTR
GTTGGTCGTGCATCCCATTC+TGG 0.363613 8_2:+71110446 Msa1266320:CDS
TTGAAGTTTGCAATCTTGTA+AGG 0.367147 8_2:-71109318 None:intergenic
TGTGCAGTGGTGGATATAAT+AGG 0.370705 8_2:+71111425 Msa1266320:three_prime_UTR
GTGGTAAGCGCTCGAGGCTC+CGG 0.373825 8_2:-71110747 None:intergenic
GGTGGATATAATAGGAAGTT+AGG 0.374612 8_2:+71111433 Msa1266320:three_prime_UTR
AGCTCAAGGTGATGCTGATT+TGG 0.377196 8_2:+71111468 Msa1266320:three_prime_UTR
GAATTCTCCACCTCTCTAGA+AGG 0.384447 8_2:-71110695 None:intergenic
ATTGATCAATTCTTGAAAGA+TGG 0.389055 8_2:+71111059 Msa1266320:CDS
CGCAGGTTTCCCCATGTACC+TGG 0.393784 8_2:+71110332 Msa1266320:intron
TTCGTACAACAAAACCAAAA+TGG 0.405006 8_2:-71111704 None:intergenic
TAATCGGTGTAGCCAATAAC+AGG 0.415841 8_2:-71111595 None:intergenic
GAACCTAAGAAAAGCTTATG+AGG 0.420831 8_2:+71111393 Msa1266320:three_prime_UTR
GATGCTGATTTGGTATCATT+TGG 0.426648 8_2:+71111478 Msa1266320:three_prime_UTR
CTGAATAAATTCCAGGTACA+TGG 0.427423 8_2:-71110343 None:intergenic
GCATCATTGTGATCAATTGC+TGG 0.434877 8_2:-71111203 None:intergenic
TGAAATCGAATGATTATTGA+AGG 0.436371 8_2:-71109148 None:intergenic
AATGATCGAACAGACGAGTA+TGG 0.441181 8_2:+71111086 Msa1266320:CDS
TATTCAGCAAGTGCTGCGTT+TGG 0.442605 8_2:-71109484 None:intergenic
AACGCTCTGCATCTCGTCAT+AGG 0.445839 8_2:-71109451 None:intergenic
TTTGGTGAGGAAAGGATAAT+CGG 0.449437 8_2:-71111611 None:intergenic
ATTGGAGTGCAGGGTGGTGT+TGG 0.449570 8_2:+71109426 Msa1266320:intron
GCTATTAATGCGATTCGAGC+AGG 0.471945 8_2:+71110810 Msa1266320:CDS
TTGATCAATTCTTGAAAGAT+GGG 0.478672 8_2:+71111060 Msa1266320:CDS
CTCAAGCCAGATGGTTCCTA+TGG 0.479424 8_2:+71110717 Msa1266320:CDS
AGCCCTTAGGAATATTATGA+TGG 0.482377 8_2:-71111776 None:intergenic
ACAAAAGATAATGCTGCCTT+TGG 0.485408 8_2:-71110411 None:intergenic
AGGTTGAGTAACATGAAGAT+AGG 0.492670 8_2:-71111308 None:intergenic
GAAACTGAGGTTACCAGAAT+GGG 0.497238 8_2:-71110459 None:intergenic
AGGCTCCGGGTATGCGCCAT+AGG 0.499161 8_2:-71110733 None:intergenic
GGTTTGGTTGTGGATGAAAT+GGG 0.499316 8_2:-71110672 None:intergenic
TTGATGGAGTCGAAATTCAC+GGG 0.503324 8_2:+71111021 Msa1266320:CDS
CATCATTGTGATCAATTGCT+GGG 0.505996 8_2:-71111202 None:intergenic
TGAAACTGAGGTTACCAGAA+TGG 0.512235 8_2:-71110460 None:intergenic
TTTGATGGAGTCGAAATTCA+CGG 0.512397 8_2:+71111020 Msa1266320:CDS
AGGAGAAAAGAGGGTGGTGC+TGG 0.515328 8_2:-71109298 None:intergenic
TGGTAAGCGCTCGAGGCTCC+GGG 0.517375 8_2:-71110746 None:intergenic
CCCATAAGCAGTGGGAGAGA+TGG 0.517721 8_2:-71109557 None:intergenic
TCATTTCCTTTGGTGAGGAA+AGG 0.518047 8_2:-71111619 None:intergenic
TCTGGAAGTTGATCCCAATC+AGG 0.520097 8_2:+71109188 Msa1266320:five_prime_UTR
CAATCTTGTAAGGAGAAAAG+AGG 0.521873 8_2:-71109308 None:intergenic
GAATCGCATTAATAGCTGAT+TGG 0.525902 8_2:-71110803 None:intergenic
GCTATTGATTCTGATCCACT+TGG 0.530394 8_2:+71111224 Msa1266320:CDS
TATGCGCCATAGGAACCATC+TGG 0.531081 8_2:-71110723 None:intergenic
GTCGAAATTCACGGGGCACA+CGG 0.533112 8_2:+71111029 Msa1266320:CDS
GAGTCTCATTTCCTTTGGTG+AGG 0.533954 8_2:-71111624 None:intergenic
ACGAGATGCAGAGCGTTGAA+TGG 0.535942 8_2:+71109457 Msa1266320:CDS
AAACAATCTCCAAAAGAGCT+TGG 0.540370 8_2:+71111274 Msa1266320:CDS
GAAATCGAATGATTATTGAA+GGG 0.540825 8_2:-71109147 None:intergenic
TTCCTGTTATACTTGTTCAG+AGG 0.544474 8_2:-71111553 None:intergenic
AATTCTCCACCTCTCTAGAA+GGG 0.550426 8_2:-71110694 None:intergenic
TCGAAATTCACGGGGCACAC+GGG 0.550944 8_2:+71111030 Msa1266320:CDS
TGAATAAATTCCAGGTACAT+GGG 0.553359 8_2:-71110342 None:intergenic
TGGTTCCTATGGCGCATACC+CGG 0.554696 8_2:+71110728 Msa1266320:CDS
GTATGACAAGGAAGAAGAAG+AGG 0.555686 8_2:+71111357 Msa1266320:CDS
GAAATGGGTGCAGCACCACC+AGG 0.555752 8_2:-71110657 None:intergenic
CAGAGGAACAAGTGGAGGCA+TGG 0.561445 8_2:+71110363 Msa1266320:CDS
GGTGGATTTCTCATCGGTGA+AGG 0.575119 8_2:+71109529 Msa1266320:CDS
GATCACTTGAAAATCGATGC+AGG 0.580483 8_2:+71111111 Msa1266320:CDS
ATTATATACCCATAAGCAGT+GGG 0.587040 8_2:-71109565 None:intergenic
ATGCAGGTTCTTAATTCAGG+TGG 0.588415 8_2:+71111127 Msa1266320:CDS
TCAGAAGTGGTAAGCGCTCG+AGG 0.590830 8_2:-71110753 None:intergenic
ATATAATAGGAAGTTAGGAA+TGG 0.592051 8_2:+71111438 Msa1266320:three_prime_UTR
GGAATGGAAGCTGTAGCTCA+AGG 0.592444 8_2:+71111454 Msa1266320:three_prime_UTR
CCAAACCCCTTCTAGAGAGG+TGG 0.592960 8_2:+71110688 Msa1266320:CDS
AGTTCAACAACACAAGTACA+AGG 0.594788 8_2:+71109270 Msa1266320:CDS
TATTTCTGGTATCTCAGAAG+TGG 0.602504 8_2:-71110766 None:intergenic
TACGCTCAAAGATCAACTCC+AGG 0.602812 8_2:+71109508 Msa1266320:CDS
GATTATCCTTTCCTCACCAA+AGG 0.604147 8_2:+71111613 Msa1266320:three_prime_UTR
GGAACATTCATGTGCAGTGG+TGG 0.609749 8_2:+71111415 Msa1266320:three_prime_UTR
TTATTCAGAGGAACAAGTGG+AGG 0.610128 8_2:+71110358 Msa1266320:CDS
AATTTATTCAGAGGAACAAG+TGG 0.610952 8_2:+71110355 Msa1266320:CDS
TTTCCATAAGCTGTATATCG+AGG 0.611276 8_2:-71111328 None:intergenic
ATAAGCAGTGGGAGAGATGG+AGG 0.611650 8_2:-71109554 None:intergenic
AAGAAGAGGCTCATTTGATG+AGG 0.613862 8_2:+71111371 Msa1266320:exon
CAACCAAACCCCTTCTAGAG+AGG 0.615926 8_2:+71110685 Msa1266320:CDS
ATTCTCCACCTCTCTAGAAG+GGG 0.617495 8_2:-71110693 None:intergenic
AACCTAAGAAAAGCTTATGA+GGG 0.618035 8_2:+71111394 Msa1266320:three_prime_UTR
TGGAGAATTCTCAAGCCAGA+TGG 0.619198 8_2:+71110708 Msa1266320:CDS
CAAAATATATATATTAACAA+TGG 0.622360 8_2:+71109230 Msa1266320:exon
GGTATTGGAGCTGAAAGAGT+TGG 0.624730 8_2:+71111167 Msa1266320:CDS
GAATAAATTCCAGGTACATG+GGG 0.626230 8_2:-71110341 None:intergenic
ACTCCAGGTGGATTTCTCAT+CGG 0.631444 8_2:+71109523 Msa1266320:CDS
AATTATATACCCATAAGCAG+TGG 0.632930 8_2:-71109566 None:intergenic
ACTGCTAAGCCTAATCCAAG+TGG 0.645958 8_2:-71111239 None:intergenic
AGCTCCAATACCAGAAACAA+CGG 0.648152 8_2:-71111156 None:intergenic
TGTGAAGCAGTGTATCAGCC+TGG 0.649524 8_2:+71110639 Msa1266320:intron
GTGGATGCTGCTCATGCCAA+AGG 0.652152 8_2:+71110395 Msa1266320:CDS
GATCGAACAGACGAGTATGG+TGG 0.652328 8_2:+71111089 Msa1266320:CDS
TGAGATACCAGAAATAGTCA+AGG 0.658712 8_2:+71110773 Msa1266320:CDS
TGTACCTGGAATTTATTCAG+AGG 0.660053 8_2:+71110346 Msa1266320:CDS
ATCATTGTGATCAATTGCTG+GGG 0.663260 8_2:-71111201 None:intergenic
TCACCGATGAGAAATCCACC+TGG 0.665351 8_2:-71109526 None:intergenic
AATCTTGTAAGGAGAAAAGA+GGG 0.669109 8_2:-71109307 None:intergenic
GGAGGCATGGAGAAATGTCG+TGG 0.680628 8_2:+71110376 Msa1266320:CDS
GCTCAAAGATCAACTCCAGG+TGG 0.683876 8_2:+71109511 Msa1266320:CDS
GAGGGAACATTCATGTGCAG+TGG 0.698747 8_2:+71111412 Msa1266320:three_prime_UTR
TGATGGAGTCGAAATTCACG+GGG 0.717446 8_2:+71111022 Msa1266320:CDS
GAAGCAGTGTATCAGCCTGG+TGG 0.735693 8_2:+71110642 Msa1266320:intron
CTTGTAAGGAGAAAAGAGGG+TGG 0.760384 8_2:-71109304 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATATATATATTTTGTGA+TGG - chr8_2:71109227-71109246 None:intergenic 10.0%
!! CAAAATATATATATTAACAA+TGG + chr8_2:71109230-71109249 Msa1266320:exon 10.0%
!!! AAATTTGGATTATTTTTTTA+AGG - chr8_2:71109736-71109755 None:intergenic 10.0%
!!! TGTAATATTTTCATATATTT+AGG - chr8_2:71110281-71110300 None:intergenic 10.0%
!! TATATGAAAATATTACATTA+TGG + chr8_2:71110284-71110303 Msa1266320:intron 10.0%
!!! TTTTTTTTTTTTTACCATTT+TGG + chr8_2:71111690-71111709 Msa1266320:three_prime_UTR 10.0%
!! AAACAGTTATAAAGTAATAA+AGG + chr8_2:71109793-71109812 Msa1266320:intron 15.0%
!!! ATAAAGTAATAAAGGTATAT+TGG + chr8_2:71109801-71109820 Msa1266320:intron 15.0%
!! ATACTGTATAAAAGAAATAT+AGG + chr8_2:71109870-71109889 Msa1266320:intron 15.0%
!! AGTACATAAAACTAAATAAA+CGG + chr8_2:71110053-71110072 Msa1266320:intron 15.0%
!!! TATTTTCATATATTTAGGAT+TGG - chr8_2:71110276-71110295 None:intergenic 15.0%
!!! AATACTACATTTTTAGATTC+TGG + chr8_2:71109170-71109189 Msa1266320:five_prime_UTR 20.0%
!!! TTTTTTTTCTCTGTTATTGT+GGG + chr8_2:71109377-71109396 Msa1266320:intron 20.0%
!! AAATAATATTATGTTGATGC+TGG + chr8_2:71109689-71109708 Msa1266320:intron 20.0%
!! ATACATCTAACAATCAAATT+TGG - chr8_2:71109751-71109770 None:intergenic 20.0%
!!! TTTATCCTTTGAAATTTGTA+AGG - chr8_2:71109846-71109865 None:intergenic 20.0%
!! TGTATAAAAGAAATATAGGA+CGG + chr8_2:71109874-71109893 Msa1266320:intron 20.0%
!! ACATTATGGTATTGTAATAT+AGG + chr8_2:71110298-71110317 Msa1266320:intron 20.0%
!! CATTATGGTATTGTAATATA+GGG + chr8_2:71110299-71110318 Msa1266320:intron 20.0%
!!! GAATGTTTTGATTATAAATG+TGG + chr8_2:71111752-71111771 Msa1266320:three_prime_UTR 20.0%
! GAAATCGAATGATTATTGAA+GGG - chr8_2:71109150-71109169 None:intergenic 25.0%
! TGAAATCGAATGATTATTGA+AGG - chr8_2:71109151-71109170 None:intergenic 25.0%
!!! GTTTTTTTTCTCTGTTATTG+TGG + chr8_2:71109376-71109395 Msa1266320:intron 25.0%
! AAAGAAAAAGCTTGAAAAAG+AGG - chr8_2:71109592-71109611 None:intergenic 25.0%
! GACATCAATGAATATAAGTT+AGG - chr8_2:71109642-71109661 None:intergenic 25.0%
! TTTGATTGTTAGATGTATGT+TGG + chr8_2:71109753-71109772 Msa1266320:intron 25.0%
! TGTATGTTGGTATTTACTAT+TGG + chr8_2:71109766-71109785 Msa1266320:intron 25.0%
! ATTGTCCTTACAAATTTCAA+AGG + chr8_2:71109838-71109857 Msa1266320:intron 25.0%
! TTGTAATATAGGGAAATGTT+TGG + chr8_2:71110309-71110328 Msa1266320:intron 25.0%
! TTCACAATTAAATCATTGAG+TGG - chr8_2:71110625-71110644 None:intergenic 25.0%
! TATTTGTTTACATCTCTTCA+AGG + chr8_2:71110838-71110857 Msa1266320:intron 25.0%
! CTCACAAACATAAAATAGTA+AGG - chr8_2:71110878-71110897 None:intergenic 25.0%
! ATTGATCAATTCTTGAAAGA+TGG + chr8_2:71111059-71111078 Msa1266320:CDS 25.0%
! TTGATCAATTCTTGAAAGAT+GGG + chr8_2:71111060-71111079 Msa1266320:CDS 25.0%
! ATATAATAGGAAGTTAGGAA+TGG + chr8_2:71111438-71111457 Msa1266320:three_prime_UTR 25.0%
! TTAAACCTTAAAACCAAATC+TGG - chr8_2:71111523-71111542 None:intergenic 25.0%
! CAAAACATTCTCTTAAGATT+TGG - chr8_2:71111742-71111761 None:intergenic 25.0%
! ATGAATTTTCTCTCCTGATT+GGG - chr8_2:71109204-71109223 None:intergenic 30.0%
AATCTTGTAAGGAGAAAAGA+GGG - chr8_2:71109310-71109329 None:intergenic 30.0%
TTGAAGTTTGCAATCTTGTA+AGG - chr8_2:71109321-71109340 None:intergenic 30.0%
! CAAACTTCAACTTTTCTCAT+AGG + chr8_2:71109331-71109350 Msa1266320:CDS 30.0%
ATTATATACCCATAAGCAGT+GGG - chr8_2:71109568-71109587 None:intergenic 30.0%
AATTATATACCCATAAGCAG+TGG - chr8_2:71109569-71109588 None:intergenic 30.0%
GAATAGTACTAAAACCATGT+AGG - chr8_2:71110092-71110111 None:intergenic 30.0%
TGAATAAATTCCAGGTACAT+GGG - chr8_2:71110345-71110364 None:intergenic 30.0%
AATTTATTCAGAGGAACAAG+TGG + chr8_2:71110355-71110374 Msa1266320:CDS 30.0%
! GCATTATCTTTTGTCAGTTA+TGG + chr8_2:71110420-71110439 Msa1266320:CDS 30.0%
! GTTTTCAATGCAGTTCATTA+AGG - chr8_2:71110512-71110531 None:intergenic 30.0%
AACCTAAGAAAAGCTTATGA+GGG + chr8_2:71111394-71111413 Msa1266320:three_prime_UTR 30.0%
TTTCATCTCTAATCCAGATT+TGG + chr8_2:71111507-71111526 Msa1266320:three_prime_UTR 30.0%
!!! CTAATCCAGATTTGGTTTTA+AGG + chr8_2:71111515-71111534 Msa1266320:three_prime_UTR 30.0%
GAAGGAAAAACAACAATCAA+AGG - chr8_2:71111666-71111685 None:intergenic 30.0%
TTCGTACAACAAAACCAAAA+TGG - chr8_2:71111707-71111726 None:intergenic 30.0%
! GATGAATTTTCTCTCCTGAT+TGG - chr8_2:71109205-71109224 None:intergenic 35.0%
AGTTCAACAACACAAGTACA+AGG + chr8_2:71109270-71109289 Msa1266320:CDS 35.0%
CAATCTTGTAAGGAGAAAAG+AGG - chr8_2:71109311-71109330 None:intergenic 35.0%
ACTATGATCTGATGTGGTTA+TGG + chr8_2:71109402-71109421 Msa1266320:intron 35.0%
ATCTGATGTGGTTATGGTAT+TGG + chr8_2:71109408-71109427 Msa1266320:intron 35.0%
! CATGCATCTGATTTTGTCAA+TGG + chr8_2:71109939-71109958 Msa1266320:intron 35.0%
AGTTGCGACAAATGAATCAA+GGG - chr8_2:71109972-71109991 None:intergenic 35.0%
AAGTTGCGACAAATGAATCA+AGG - chr8_2:71109973-71109992 None:intergenic 35.0%
CACATGACAAAAAGAAACTC+CGG - chr8_2:71110004-71110023 None:intergenic 35.0%
GTCAAACAAAAAGTCCTACA+TGG + chr8_2:71110075-71110094 Msa1266320:intron 35.0%
TTAAACACAATCAGCCTCAT+TGG - chr8_2:71110127-71110146 None:intergenic 35.0%
ATCAACGACAAAAAGAACAC+AGG - chr8_2:71110170-71110189 None:intergenic 35.0%
GAATAAATTCCAGGTACATG+GGG - chr8_2:71110344-71110363 None:intergenic 35.0%
CTGAATAAATTCCAGGTACA+TGG - chr8_2:71110346-71110365 None:intergenic 35.0%
TGTACCTGGAATTTATTCAG+AGG + chr8_2:71110346-71110365 Msa1266320:CDS 35.0%
TGTTCCTCTGAATAAATTCC+AGG - chr8_2:71110353-71110372 None:intergenic 35.0%
ACAAAAGATAATGCTGCCTT+TGG - chr8_2:71110414-71110433 None:intergenic 35.0%
! TTTTGTCAGTTATGGCATGT+TGG + chr8_2:71110428-71110447 Msa1266320:CDS 35.0%
! CATTTAGGATAGTGAAACTG+AGG - chr8_2:71110475-71110494 None:intergenic 35.0%
! GATGAACTTGTGAAGCATTT+AGG - chr8_2:71110490-71110509 None:intergenic 35.0%
!! TATTTCTGGTATCTCAGAAG+TGG - chr8_2:71110769-71110788 None:intergenic 35.0%
TGAGATACCAGAAATAGTCA+AGG + chr8_2:71110773-71110792 Msa1266320:CDS 35.0%
CGATAATCCTTGACTATTTC+TGG - chr8_2:71110783-71110802 None:intergenic 35.0%
GAATCGCATTAATAGCTGAT+TGG - chr8_2:71110806-71110825 None:intergenic 35.0%
ATGAAATGAGACGTACATAC+AGG + chr8_2:71110996-71111015 Msa1266320:intron 35.0%
TTTGATGGAGTCGAAATTCA+CGG + chr8_2:71111020-71111039 Msa1266320:CDS 35.0%
ATCATTGTGATCAATTGCTG+GGG - chr8_2:71111204-71111223 None:intergenic 35.0%
CATCATTGTGATCAATTGCT+GGG - chr8_2:71111205-71111224 None:intergenic 35.0%
AAACAATCTCCAAAAGAGCT+TGG + chr8_2:71111274-71111293 Msa1266320:CDS 35.0%
! AGTTTTCTACCAAGCTCTTT+TGG - chr8_2:71111286-71111305 None:intergenic 35.0%
AGGTTGAGTAACATGAAGAT+AGG - chr8_2:71111311-71111330 None:intergenic 35.0%
TTTCCATAAGCTGTATATCG+AGG - chr8_2:71111331-71111350 None:intergenic 35.0%
TGGAAAAACTGAGTATGACA+AGG + chr8_2:71111345-71111364 Msa1266320:CDS 35.0%
GAACCTAAGAAAAGCTTATG+AGG + chr8_2:71111393-71111412 Msa1266320:three_prime_UTR 35.0%
! TTCCCTCATAAGCTTTTCTT+AGG - chr8_2:71111399-71111418 None:intergenic 35.0%
GGTGGATATAATAGGAAGTT+AGG + chr8_2:71111433-71111452 Msa1266320:three_prime_UTR 35.0%
!! GATGCTGATTTGGTATCATT+TGG + chr8_2:71111478-71111497 Msa1266320:three_prime_UTR 35.0%
TTCCTGTTATACTTGTTCAG+AGG - chr8_2:71111556-71111575 None:intergenic 35.0%
TTTGGTGAGGAAAGGATAAT+CGG - chr8_2:71111614-71111633 None:intergenic 35.0%
!!! GGTTTTGTTGTACGAACAAT+TGG + chr8_2:71111711-71111730 Msa1266320:three_prime_UTR 35.0%
! CATCTGATTTTGTCAATGGC+CGG + chr8_2:71109943-71109962 Msa1266320:intron 40.0%
! TGTCGCAACTTCTTTTTGAC+CGG + chr8_2:71109982-71110001 Msa1266320:intron 40.0%
TTATTCAGAGGAACAAGTGG+AGG + chr8_2:71110358-71110377 Msa1266320:CDS 40.0%
GAAACTGAGGTTACCAGAAT+GGG - chr8_2:71110462-71110481 None:intergenic 40.0%
TGAAACTGAGGTTACCAGAA+TGG - chr8_2:71110463-71110482 None:intergenic 40.0%
! GGTTTGGTTGTGGATGAAAT+GGG - chr8_2:71110675-71110694 None:intergenic 40.0%
! AATTCTCCACCTCTCTAGAA+GGG - chr8_2:71110697-71110716 None:intergenic 40.0%
!! GACGTACATACAGGTTTTGA+TGG + chr8_2:71111005-71111024 Msa1266320:intron 40.0%
TTGATGGAGTCGAAATTCAC+GGG + chr8_2:71111021-71111040 Msa1266320:CDS 40.0%
AATGATCGAACAGACGAGTA+TGG + chr8_2:71111086-71111105 Msa1266320:CDS 40.0%
GATCACTTGAAAATCGATGC+AGG + chr8_2:71111111-71111130 Msa1266320:CDS 40.0%
TCGATGCAGGTTCTTAATTC+AGG + chr8_2:71111124-71111143 Msa1266320:CDS 40.0%
ATGCAGGTTCTTAATTCAGG+TGG + chr8_2:71111127-71111146 Msa1266320:CDS 40.0%
GGTTCTTAATTCAGGTGGTT+CGG + chr8_2:71111132-71111151 Msa1266320:CDS 40.0%
GTTCTTAATTCAGGTGGTTC+GGG + chr8_2:71111133-71111152 Msa1266320:CDS 40.0%
AGCTCCAATACCAGAAACAA+CGG - chr8_2:71111159-71111178 None:intergenic 40.0%
GCATCATTGTGATCAATTGC+TGG - chr8_2:71111206-71111225 None:intergenic 40.0%
!! GCTATTGATTCTGATCCACT+TGG + chr8_2:71111224-71111243 Msa1266320:CDS 40.0%
GATTCTGATCCACTTGGATT+AGG + chr8_2:71111230-71111249 Msa1266320:CDS 40.0%
CAACCTCGATATACAGCTTA+TGG + chr8_2:71111325-71111344 Msa1266320:CDS 40.0%
GTATGACAAGGAAGAAGAAG+AGG + chr8_2:71111357-71111376 Msa1266320:CDS 40.0%
AAGAAGAGGCTCATTTGATG+AGG + chr8_2:71111371-71111390 Msa1266320:exon 40.0%
!! TGTGCAGTGGTGGATATAAT+AGG + chr8_2:71111425-71111444 Msa1266320:three_prime_UTR 40.0%
CTCCTCTGAACAAGTATAAC+AGG + chr8_2:71111551-71111570 Msa1266320:three_prime_UTR 40.0%
! CAGGAAGACATTTTACACAC+AGG + chr8_2:71111570-71111589 Msa1266320:three_prime_UTR 40.0%
TACACACAGGATCCTGTTAT+TGG + chr8_2:71111583-71111602 Msa1266320:three_prime_UTR 40.0%
TAATCGGTGTAGCCAATAAC+AGG - chr8_2:71111598-71111617 None:intergenic 40.0%
GATTATCCTTTCCTCACCAA+AGG + chr8_2:71111613-71111632 Msa1266320:three_prime_UTR 40.0%
TCATTTCCTTTGGTGAGGAA+AGG - chr8_2:71111622-71111641 None:intergenic 40.0%
CTCTTGAGTCTCATTTCCTT+TGG - chr8_2:71111632-71111651 None:intergenic 40.0%
! TCTGGAAGTTGATCCCAATC+AGG + chr8_2:71109188-71109207 Msa1266320:five_prime_UTR 45.0%
CTTGTAAGGAGAAAAGAGGG+TGG - chr8_2:71109307-71109326 None:intergenic 45.0%
TGGGTGACTATGATCTGATG+TGG + chr8_2:71109396-71109415 Msa1266320:intron 45.0%
! TGGTTATGGTATTGGAGTGC+AGG + chr8_2:71109416-71109435 Msa1266320:intron 45.0%
! GGTTATGGTATTGGAGTGCA+GGG + chr8_2:71109417-71109436 Msa1266320:intron 45.0%
! TATTCAGCAAGTGCTGCGTT+TGG - chr8_2:71109487-71109506 None:intergenic 45.0%
TACGCTCAAAGATCAACTCC+AGG + chr8_2:71109508-71109527 Msa1266320:CDS 45.0%
! ACTCCAGGTGGATTTCTCAT+CGG + chr8_2:71109523-71109542 Msa1266320:CDS 45.0%
ACAAATGAATCAAGGGTGGC+CGG - chr8_2:71109965-71109984 None:intergenic 45.0%
TGCGACAAATGAATCAAGGG+TGG - chr8_2:71109969-71109988 None:intergenic 45.0%
TCTATCGCTACCAACCAATG+AGG + chr8_2:71110110-71110129 Msa1266320:intron 45.0%
ACACAATCAGCCTCATTGGT+TGG - chr8_2:71110123-71110142 None:intergenic 45.0%
TATAGGGAAATGTTTGGCGC+AGG + chr8_2:71110315-71110334 Msa1266320:intron 45.0%
! GGGTTTGGTTGTGGATGAAA+TGG - chr8_2:71110676-71110695 None:intergenic 45.0%
! ATTCTCCACCTCTCTAGAAG+GGG - chr8_2:71110696-71110715 None:intergenic 45.0%
GAATTCTCCACCTCTCTAGA+AGG - chr8_2:71110698-71110717 None:intergenic 45.0%
TGGAGAATTCTCAAGCCAGA+TGG + chr8_2:71110708-71110727 Msa1266320:CDS 45.0%
GCTATTAATGCGATTCGAGC+AGG + chr8_2:71110810-71110829 Msa1266320:CDS 45.0%
ATACTCGTGTTGCATCTGCA+CGG + chr8_2:71110922-71110941 Msa1266320:intron 45.0%
TGATGGAGTCGAAATTCACG+GGG + chr8_2:71111022-71111041 Msa1266320:CDS 45.0%
! GGTATTGGAGCTGAAAGAGT+TGG + chr8_2:71111167-71111186 Msa1266320:CDS 45.0%
ACTGCTAAGCCTAATCCAAG+TGG - chr8_2:71111242-71111261 None:intergenic 45.0%
! AGCTCAAGGTGATGCTGATT+TGG + chr8_2:71111468-71111487 Msa1266320:three_prime_UTR 45.0%
GAGTCTCATTTCCTTTGGTG+AGG - chr8_2:71111627-71111646 None:intergenic 45.0%
!! TAAAAAAAAAAAAAAAAAGA+AGG - chr8_2:71111684-71111703 None:intergenic 5.0%
! TATGGTATTGGAGTGCAGGG+TGG + chr8_2:71109420-71109439 Msa1266320:intron 50.0%
AACGCTCTGCATCTCGTCAT+AGG - chr8_2:71109454-71109473 None:intergenic 50.0%
!! ACGAGATGCAGAGCGTTGAA+TGG + chr8_2:71109457-71109476 Msa1266320:CDS 50.0%
GCTCAAAGATCAACTCCAGG+TGG + chr8_2:71109511-71109530 Msa1266320:CDS 50.0%
TCACCGATGAGAAATCCACC+TGG - chr8_2:71109529-71109548 None:intergenic 50.0%
! GGTGGATTTCTCATCGGTGA+AGG + chr8_2:71109529-71109548 Msa1266320:CDS 50.0%
ATAAGCAGTGGGAGAGATGG+AGG - chr8_2:71109557-71109576 None:intergenic 50.0%
TCCATCTCTCCCACTGCTTA+TGG + chr8_2:71109556-71109575 Msa1266320:CDS 50.0%
CCATCTCTCCCACTGCTTAT+GGG + chr8_2:71109557-71109576 Msa1266320:CDS 50.0%
TGTGAAGCAGTGTATCAGCC+TGG + chr8_2:71110639-71110658 Msa1266320:intron 50.0%
! CTCTAGAAGGGGTTTGGTTG+TGG - chr8_2:71110685-71110704 None:intergenic 50.0%
! CAACCAAACCCCTTCTAGAG+AGG + chr8_2:71110685-71110704 Msa1266320:CDS 50.0%
CTCAAGCCAGATGGTTCCTA+TGG + chr8_2:71110717-71110736 Msa1266320:CDS 50.0%
TATGCGCCATAGGAACCATC+TGG - chr8_2:71110726-71110745 None:intergenic 50.0%
GTGCAGATGCAACACGAGTA+TGG - chr8_2:71110923-71110942 None:intergenic 50.0%
TACTCGTGTTGCATCTGCAC+GGG + chr8_2:71110923-71110942 Msa1266320:intron 50.0%
TGTTGCATCTGCACGGGTAT+GGG + chr8_2:71110929-71110948 Msa1266320:intron 50.0%
GATCGAACAGACGAGTATGG+TGG + chr8_2:71111089-71111108 Msa1266320:CDS 50.0%
GAGGGAACATTCATGTGCAG+TGG + chr8_2:71111412-71111431 Msa1266320:three_prime_UTR 50.0%
GGAACATTCATGTGCAGTGG+TGG + chr8_2:71111415-71111434 Msa1266320:three_prime_UTR 50.0%
GGAATGGAAGCTGTAGCTCA+AGG + chr8_2:71111454-71111473 Msa1266320:three_prime_UTR 50.0%
AGGAGAAAAGAGGGTGGTGC+TGG - chr8_2:71109301-71109320 None:intergenic 55.0%
ATTGGAGTGCAGGGTGGTGT+TGG + chr8_2:71109426-71109445 Msa1266320:intron 55.0%
CCCATAAGCAGTGGGAGAGA+TGG - chr8_2:71109560-71109579 None:intergenic 55.0%
CAGAGGAACAAGTGGAGGCA+TGG + chr8_2:71110363-71110382 Msa1266320:CDS 55.0%
! GGAGGCATGGAGAAATGTCG+TGG + chr8_2:71110376-71110395 Msa1266320:CDS 55.0%
GTGGATGCTGCTCATGCCAA+AGG + chr8_2:71110395-71110414 Msa1266320:CDS 55.0%
GTTGGTCGTGCATCCCATTC+TGG + chr8_2:71110446-71110465 Msa1266320:CDS 55.0%
GAAGCAGTGTATCAGCCTGG+TGG + chr8_2:71110642-71110661 Msa1266320:intron 55.0%
! CCACCTCTCTAGAAGGGGTT+TGG - chr8_2:71110691-71110710 None:intergenic 55.0%
! CCAAACCCCTTCTAGAGAGG+TGG + chr8_2:71110688-71110707 Msa1266320:CDS 55.0%
TGGTTCCTATGGCGCATACC+CGG + chr8_2:71110728-71110747 Msa1266320:CDS 55.0%
TCAGAAGTGGTAAGCGCTCG+AGG - chr8_2:71110756-71110775 None:intergenic 55.0%
GTGTTGCATCTGCACGGGTA+TGG + chr8_2:71110928-71110947 Msa1266320:intron 55.0%
GTCGAAATTCACGGGGCACA+CGG + chr8_2:71111029-71111048 Msa1266320:CDS 55.0%
TCGAAATTCACGGGGCACAC+GGG + chr8_2:71111030-71111049 Msa1266320:CDS 55.0%
!! CGGGCCGTTGTTTCTGGTAT+TGG + chr8_2:71111152-71111171 Msa1266320:CDS 55.0%
CGCAGGTTTCCCCATGTACC+TGG + chr8_2:71110332-71110351 Msa1266320:intron 60.0%
GAAATGGGTGCAGCACCACC+AGG - chr8_2:71110660-71110679 None:intergenic 60.0%
! GTGGTTCGGGCCGTTGTTTC+TGG + chr8_2:71111146-71111165 Msa1266320:CDS 60.0%
AGGCTCCGGGTATGCGCCAT+AGG - chr8_2:71110736-71110755 None:intergenic 65.0%
TGGTAAGCGCTCGAGGCTCC+GGG - chr8_2:71110749-71110768 None:intergenic 65.0%
GTGGTAAGCGCTCGAGGCTC+CGG - chr8_2:71110750-71110769 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 71109140 71111787 71109140 ID=Msa1266320;Name=Msa1266320
chr8_2 mRNA 71109140 71111787 71109140 ID=Msa1266320-mRNA-1;Parent=Msa1266320;Name=Msa1266320-mRNA-1;_AED=0.04;_eAED=0.04;_QI=109|1|1|1|1|1|5|399|309
chr8_2 exon 71109140 71109352 71109140 ID=Msa1266320-mRNA-1:exon:23735;Parent=Msa1266320-mRNA-1
chr8_2 exon 71109438 71109578 71109438 ID=Msa1266320-mRNA-1:exon:23736;Parent=Msa1266320-mRNA-1
chr8_2 exon 71110337 71110467 71110337 ID=Msa1266320-mRNA-1:exon:23737;Parent=Msa1266320-mRNA-1
chr8_2 exon 71110649 71110831 71110649 ID=Msa1266320-mRNA-1:exon:23738;Parent=Msa1266320-mRNA-1
chr8_2 exon 71111018 71111787 71111018 ID=Msa1266320-mRNA-1:exon:23739;Parent=Msa1266320-mRNA-1
chr8_2 five_prime_UTR 71109140 71109248 71109140 ID=Msa1266320-mRNA-1:five_prime_utr;Parent=Msa1266320-mRNA-1
chr8_2 CDS 71109249 71109352 71109249 ID=Msa1266320-mRNA-1:cds;Parent=Msa1266320-mRNA-1
chr8_2 CDS 71109438 71109578 71109438 ID=Msa1266320-mRNA-1:cds;Parent=Msa1266320-mRNA-1
chr8_2 CDS 71110337 71110467 71110337 ID=Msa1266320-mRNA-1:cds;Parent=Msa1266320-mRNA-1
chr8_2 CDS 71110649 71110831 71110649 ID=Msa1266320-mRNA-1:cds;Parent=Msa1266320-mRNA-1
chr8_2 CDS 71111018 71111388 71111018 ID=Msa1266320-mRNA-1:cds;Parent=Msa1266320-mRNA-1
chr8_2 three_prime_UTR 71111389 71111787 71111389 ID=Msa1266320-mRNA-1:three_prime_utr;Parent=Msa1266320-mRNA-1
Gene Sequence

>Msa1266320

ATGGCAGAGTACAATAATTCAAGTTCAACAACACAAGTACAAGGAGACACCAGCACCACCCTCTTTTCTCCTTACAAGATTGCAAACTTCAACTTTTCTCATAGGGTGGTGTTGGCACCTATGACGAGATGCAGAGCGTTGAATGGTATTCCAAACGCAGCACTTGCTGAATACTACGCTCAAAGATCAACTCCAGGTGGATTTCTCATCGGTGAAGGAACCTCCATCTCTCCCACTGCTTATGGGTTTCCCCATGTACCTGGAATTTATTCAGAGGAACAAGTGGAGGCATGGAGAAATGTCGTGGATGCTGCTCATGCCAAAGGCAGCATTATCTTTTGTCAGTTATGGCATGTTGGTCGTGCATCCCATTCTGTGTATCAGCCTGGTGGTGCTGCACCCATTTCATCCACAACCAAACCCCTTCTAGAGAGGTGGAGAATTCTCAAGCCAGATGGTTCCTATGGCGCATACCCGGAGCCTCGAGCGCTTACCACTTCTGAGATACCAGAAATAGTCAAGGATTATCGCCAATCAGCTATTAATGCGATTCGAGCAGGTTTTGATGGAGTCGAAATTCACGGGGCACACGGGTATCTGATTGATCAATTCTTGAAAGATGGGATCAATGATCGAACAGACGAGTATGGTGGATCACTTGAAAATCGATGCAGGTTCTTAATTCAGGTGGTTCGGGCCGTTGTTTCTGGTATTGGAGCTGAAAGAGTTGGTGTCAGAATTTCCCCAGCAATTGATCACAATGATGCTATTGATTCTGATCCACTTGGATTAGGCTTAGCAGTGATTGAGAGACTAAACAATCTCCAAAAGAGCTTGGTAGAAAACTCACCTATCTTCATGTTACTCAACCTCGATATACAGCTTATGGAAAAACTGAGTATGACAAGGAAGAAGAAGAGGCTCATTTGA
Protein sequence

>Msa1266320

MAEYNNSSSTTQVQGDTSTTLFSPYKIANFNFSHRVVLAPMTRCRALNGIPNAALAEYYAQRSTPGGFLIGEGTSISPTAYGFPHVPGIYSEEQVEAWRNVVDAAHAKGSIIFCQLWHVGRASHSVYQPGGAAPISSTTKPLLERWRILKPDGSYGAYPEPRALTTSEIPEIVKDYRQSAINAIRAGFDGVEIHGAHGYLIDQFLKDGINDRTDEYGGSLENRCRFLIQVVRAVVSGIGAERVGVRISPAIDHNDAIDSDPLGLGLAVIERLNNLQKSLVENSPIFMLLNLDIQLMEKLSMTRKKKRLI*