AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0583140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTCCAGTTTATTAATT+TGG 0.140240 4_2:-1587052 Msa0583140:five_prime_UTR
ATTCTTCAGGGTGCTGTTTC+CGG 0.182480 4_2:+1585820 None:intergenic
GAAGATTAACCGTAGTTTAA+TGG 0.206013 4_2:-1586744 Msa0583140:five_prime_UTR
ATATTTCCAGTTTAATTAAA+AGG 0.245381 4_2:+1586699 None:intergenic
ATTGATCACTCAAGGCAATT+TGG 0.255887 4_2:-1585853 Msa0583140:three_prime_UTR
CCTTGAGTGATCAATTTCTA+TGG 0.275219 4_2:+1585861 None:intergenic
TAGCAATGTTCAAATTCTTC+AGG 0.275366 4_2:+1585807 None:intergenic
TATATTCCAAATTAATAAAC+TGG 0.334256 4_2:+1587046 None:intergenic
AAATAAGTACAATGGAATTA+TGG 0.343125 4_2:-1586548 Msa0583140:exon
TACAATGGAATTATGGAAGT+TGG 0.379712 4_2:-1586541 Msa0583140:exon
CAAGGCAATTTGGACTATAA+TGG 0.384200 4_2:-1585843 Msa0583140:three_prime_UTR
CATTGCTATAATCCTATAGT+TGG 0.391238 4_2:-1585792 Msa0583140:three_prime_UTR
ATCAGTTTAAATCCAACTAT+AGG 0.412017 4_2:+1585780 None:intergenic
AACTGGAAATATTTGAGAGC+TGG 0.415904 4_2:-1586688 Msa0583140:five_prime_UTR
GGATTTAAACTGATGACCTT+AGG 0.438673 4_2:-1585771 Msa0583140:three_prime_UTR
ACTGGAAATATTTGAGAGCT+GGG 0.463256 4_2:-1586687 Msa0583140:five_prime_UTR
CAAAGAGAAGAAGCTGCAAA+AGG 0.479266 4_2:+1587140 None:intergenic
CAGTGTTTCAGTTTAACCTA+AGG 0.497072 4_2:+1585755 None:intergenic
TGTGTTCACCTTCTAAGAAA+AGG 0.512552 4_2:+1586606 None:intergenic
GACATGGATGATTTGTCCAC+CGG 0.528848 4_2:-1585910 Msa0583140:CDS
AGCAATGTTCAAATTCTTCA+GGG 0.534834 4_2:+1585808 None:intergenic
TAAAGCTGAATTATATTCTG+TGG 0.543595 4_2:+1586572 None:intergenic
CAATGGTAAATGAAGAAGAA+TGG 0.546725 4_2:+1587169 None:intergenic
CTTTGTTGATCAATGAGACA+TGG 0.564063 4_2:-1585926 Msa0583140:CDS
ATCGACCTTGTACAAAATGC+AGG 0.570197 4_2:-1586490 Msa0583140:intron
CTAGCACGGATCACCGTTGT+GGG 0.575018 4_2:-1586650 Msa0583140:five_prime_UTR
CCATAGAAATTGATCACTCA+AGG 0.576539 4_2:-1585861 Msa0583140:exon
ACATAGAGGCCATTAAACTA+CGG 0.579332 4_2:+1586735 None:intergenic
GCAATTTGGACTATAATGGC+CGG 0.581730 4_2:-1585839 Msa0583140:three_prime_UTR
ACATGGATGATTTGTCCACC+GGG 0.590553 4_2:-1585909 Msa0583140:CDS
ACTAGCACGGATCACCGTTG+TGG 0.591441 4_2:-1586651 Msa0583140:five_prime_UTR
AGCTTTACAAATAAGTACAA+TGG 0.598879 4_2:-1586556 Msa0583140:five_prime_UTR
GATTGTATGATACGCTAGAA+CGG 0.606609 4_2:+1587091 None:intergenic
TGAGTCTTACGACAAAATCC+CGG 0.608610 4_2:+1585891 None:intergenic
GCTGCAAAAGGAAGAAGCAA+TGG 0.610138 4_2:+1587152 None:intergenic
AATTAATCAATATTAAGCTG+TGG 0.613201 4_2:+1587069 None:intergenic
GTCTTACGACAAAATCCCGG+TGG 0.729703 4_2:+1585894 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCCAGTTTATTAATT+TGG - chr4_2:1585748-1585767 Msa0583140:three_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG + chr4_2:1585757-1585776 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG + chr4_2:1585931-1585950 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG + chr4_2:1585996-1586015 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG + chr4_2:1586104-1586123 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG - chr4_2:1586333-1586352 Msa0583140:intron 15.0%
!! TTAATTCCAGTTTATTAATT+TGG - chr4_2:1585748-1585767 Msa0583140:three_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG + chr4_2:1585757-1585776 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG + chr4_2:1585931-1585950 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG + chr4_2:1585996-1586015 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG + chr4_2:1586104-1586123 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG - chr4_2:1586333-1586352 Msa0583140:intron 15.0%
!! AATTAATCAATATTAAGCTG+TGG + chr4_2:1585734-1585753 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG + chr4_2:1586011-1586030 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG - chr4_2:1586095-1586114 Msa0583140:intron 20.0%
!! AAATAAGTACAATGGAATTA+TGG - chr4_2:1586252-1586271 Msa0583140:intron 20.0%
!! AAATATACAGTATCCAAAAT+AGG + chr4_2:1586382-1586401 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG - chr4_2:1586383-1586402 Msa0583140:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG - chr4_2:1586384-1586403 Msa0583140:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG + chr4_2:1586420-1586439 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG - chr4_2:1586437-1586456 Msa0583140:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG + chr4_2:1586485-1586504 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG - chr4_2:1586543-1586562 Msa0583140:exon 20.0%
!!! AAACATATTGGTGTTAAATT+TGG - chr4_2:1586555-1586574 Msa0583140:five_prime_UTR 20.0%
!! TGAATTATTATAACTACACT+TGG - chr4_2:1586658-1586677 Msa0583140:five_prime_UTR 20.0%
!! GAATTATTATAACTACACTT+GGG - chr4_2:1586659-1586678 Msa0583140:five_prime_UTR 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG - chr4_2:1586684-1586703 Msa0583140:five_prime_UTR 20.0%
!!! TTTTCTTTTCATTGATTTCA+TGG - chr4_2:1586726-1586745 Msa0583140:five_prime_UTR 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG + chr4_2:1587091-1587110 None:intergenic 20.0%
!! AATTAATCAATATTAAGCTG+TGG + chr4_2:1585734-1585753 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG + chr4_2:1586011-1586030 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG - chr4_2:1586095-1586114 Msa0583140:intron 20.0%
!! AAATAAGTACAATGGAATTA+TGG - chr4_2:1586252-1586271 Msa0583140:intron 20.0%
!! AAATATACAGTATCCAAAAT+AGG + chr4_2:1586382-1586401 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG - chr4_2:1586383-1586402 Msa0583140:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG - chr4_2:1586384-1586403 Msa0583140:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG + chr4_2:1586420-1586439 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG - chr4_2:1586437-1586456 Msa0583140:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG + chr4_2:1586485-1586504 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG - chr4_2:1586543-1586562 Msa0583140:exon 20.0%
!!! AAACATATTGGTGTTAAATT+TGG - chr4_2:1586555-1586574 Msa0583140:five_prime_UTR 20.0%
!! TGAATTATTATAACTACACT+TGG - chr4_2:1586658-1586677 Msa0583140:five_prime_UTR 20.0%
!! GAATTATTATAACTACACTT+GGG - chr4_2:1586659-1586678 Msa0583140:five_prime_UTR 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG - chr4_2:1586684-1586703 Msa0583140:five_prime_UTR 20.0%
!!! TTTTCTTTTCATTGATTTCA+TGG - chr4_2:1586726-1586745 Msa0583140:five_prime_UTR 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG + chr4_2:1587091-1587110 None:intergenic 20.0%
!!! GAATATAGATGTTTTGATAG+AGG - chr4_2:1585770-1585789 Msa0583140:three_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG + chr4_2:1585796-1585815 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG + chr4_2:1585964-1585983 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG + chr4_2:1585991-1586010 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG + chr4_2:1586082-1586101 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG - chr4_2:1586136-1586155 Msa0583140:intron 25.0%
! TAAAGCTGAATTATATTCTG+TGG + chr4_2:1586231-1586250 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG - chr4_2:1586244-1586263 Msa0583140:intron 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG - chr4_2:1586344-1586363 Msa0583140:intron 25.0%
!!! GTATTTTTGGAAACCTATTT+TGG - chr4_2:1586366-1586385 Msa0583140:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG - chr4_2:1586385-1586404 Msa0583140:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG + chr4_2:1587023-1587042 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG - chr4_2:1587076-1587095 Msa0583140:five_prime_UTR 25.0%
!!! GAATATAGATGTTTTGATAG+AGG - chr4_2:1585770-1585789 Msa0583140:three_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG + chr4_2:1585796-1585815 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG + chr4_2:1585964-1585983 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG + chr4_2:1585991-1586010 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG + chr4_2:1586082-1586101 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG - chr4_2:1586136-1586155 Msa0583140:intron 25.0%
! TAAAGCTGAATTATATTCTG+TGG + chr4_2:1586231-1586250 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG - chr4_2:1586244-1586263 Msa0583140:intron 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG - chr4_2:1586344-1586363 Msa0583140:intron 25.0%
!!! GTATTTTTGGAAACCTATTT+TGG - chr4_2:1586366-1586385 Msa0583140:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG - chr4_2:1586385-1586404 Msa0583140:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG + chr4_2:1587023-1587042 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG - chr4_2:1587076-1587095 Msa0583140:five_prime_UTR 25.0%
CAATGGTAAATGAAGAAGAA+TGG + chr4_2:1585634-1585653 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG - chr4_2:1585951-1585970 Msa0583140:CDS 30.0%
GAAGATTAACCGTAGTTTAA+TGG - chr4_2:1586056-1586075 Msa0583140:intron 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG - chr4_2:1586186-1586205 Msa0583140:intron 30.0%
TACAATGGAATTATGGAAGT+TGG - chr4_2:1586259-1586278 Msa0583140:intron 30.0%
ATTATGGAAGTTGGAAAAAG+TGG - chr4_2:1586268-1586287 Msa0583140:intron 30.0%
! AGGTAAAATGCAGGTATTTT+TGG - chr4_2:1586353-1586372 Msa0583140:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG - chr4_2:1586386-1586405 Msa0583140:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG - chr4_2:1586701-1586720 Msa0583140:five_prime_UTR 30.0%
AGCAATGTTCAAATTCTTCA+GGG + chr4_2:1586995-1587014 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG + chr4_2:1586996-1587015 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG - chr4_2:1587008-1587027 Msa0583140:five_prime_UTR 30.0%
CAATGGTAAATGAAGAAGAA+TGG + chr4_2:1585634-1585653 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG - chr4_2:1585951-1585970 Msa0583140:CDS 30.0%
GAAGATTAACCGTAGTTTAA+TGG - chr4_2:1586056-1586075 Msa0583140:intron 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG - chr4_2:1586186-1586205 Msa0583140:intron 30.0%
TACAATGGAATTATGGAAGT+TGG - chr4_2:1586259-1586278 Msa0583140:intron 30.0%
ATTATGGAAGTTGGAAAAAG+TGG - chr4_2:1586268-1586287 Msa0583140:intron 30.0%
! AGGTAAAATGCAGGTATTTT+TGG - chr4_2:1586353-1586372 Msa0583140:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG - chr4_2:1586386-1586405 Msa0583140:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG - chr4_2:1586701-1586720 Msa0583140:five_prime_UTR 30.0%
AGCAATGTTCAAATTCTTCA+GGG + chr4_2:1586995-1587014 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG + chr4_2:1586996-1587015 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG - chr4_2:1587008-1587027 Msa0583140:five_prime_UTR 30.0%
GATTGTATGATACGCTAGAA+CGG + chr4_2:1585712-1585731 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG + chr4_2:1586068-1586087 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG - chr4_2:1586112-1586131 Msa0583140:intron 35.0%
ACTGGAAATATTTGAGAGCT+GGG - chr4_2:1586113-1586132 Msa0583140:intron 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG + chr4_2:1586166-1586185 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG + chr4_2:1586197-1586216 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG + chr4_2:1586318-1586337 None:intergenic 35.0%
!!! GGATACTGTATATTTTTGGG+GGG - chr4_2:1586387-1586406 Msa0583140:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG - chr4_2:1586388-1586407 Msa0583140:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG - chr4_2:1586584-1586603 Msa0583140:five_prime_UTR 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG - chr4_2:1586781-1586800 Msa0583140:five_prime_UTR 35.0%
TCGTGGTGTTTATTTGTTTC+AGG - chr4_2:1586798-1586817 Msa0583140:five_prime_UTR 35.0%
CTTTGTTGATCAATGAGACA+TGG - chr4_2:1586874-1586893 Msa0583140:five_prime_UTR 35.0%
CCTTGAGTGATCAATTTCTA+TGG + chr4_2:1586942-1586961 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG - chr4_2:1586939-1586958 Msa0583140:five_prime_UTR 35.0%
ATTGATCACTCAAGGCAATT+TGG - chr4_2:1586947-1586966 Msa0583140:five_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG - chr4_2:1586957-1586976 Msa0583140:five_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG - chr4_2:1587029-1587048 Msa0583140:five_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG + chr4_2:1587048-1587067 None:intergenic 35.0%
GATTGTATGATACGCTAGAA+CGG + chr4_2:1585712-1585731 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG + chr4_2:1586068-1586087 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG - chr4_2:1586112-1586131 Msa0583140:intron 35.0%
ACTGGAAATATTTGAGAGCT+GGG - chr4_2:1586113-1586132 Msa0583140:intron 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG + chr4_2:1586166-1586185 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG + chr4_2:1586197-1586216 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG + chr4_2:1586318-1586337 None:intergenic 35.0%
!!! GGATACTGTATATTTTTGGG+GGG - chr4_2:1586387-1586406 Msa0583140:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG - chr4_2:1586388-1586407 Msa0583140:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG - chr4_2:1586584-1586603 Msa0583140:five_prime_UTR 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG - chr4_2:1586781-1586800 Msa0583140:five_prime_UTR 35.0%
TCGTGGTGTTTATTTGTTTC+AGG - chr4_2:1586798-1586817 Msa0583140:five_prime_UTR 35.0%
CTTTGTTGATCAATGAGACA+TGG - chr4_2:1586874-1586893 Msa0583140:five_prime_UTR 35.0%
CCTTGAGTGATCAATTTCTA+TGG + chr4_2:1586942-1586961 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG - chr4_2:1586939-1586958 Msa0583140:five_prime_UTR 35.0%
ATTGATCACTCAAGGCAATT+TGG - chr4_2:1586947-1586966 Msa0583140:five_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG - chr4_2:1586957-1586976 Msa0583140:five_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG - chr4_2:1587029-1587048 Msa0583140:five_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG + chr4_2:1587048-1587067 None:intergenic 35.0%
CAAAGAGAAGAAGCTGCAAA+AGG + chr4_2:1585663-1585682 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG - chr4_2:1585661-1585680 Msa0583140:three_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG - chr4_2:1585776-1585795 Msa0583140:three_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG - chr4_2:1586282-1586301 Msa0583140:intron 40.0%
ATCGACCTTGTACAAAATGC+AGG - chr4_2:1586310-1586329 Msa0583140:intron 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG - chr4_2:1586572-1586591 Msa0583140:five_prime_UTR 40.0%
TATCACACACACGTGACATA+TGG - chr4_2:1586617-1586636 Msa0583140:five_prime_UTR 40.0%
TGAGTCTTACGACAAAATCC+CGG + chr4_2:1586912-1586931 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG - chr4_2:1586961-1586980 Msa0583140:five_prime_UTR 40.0%
CAAAGAGAAGAAGCTGCAAA+AGG + chr4_2:1585663-1585682 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG - chr4_2:1585661-1585680 Msa0583140:three_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG - chr4_2:1585776-1585795 Msa0583140:three_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG - chr4_2:1586282-1586301 Msa0583140:intron 40.0%
ATCGACCTTGTACAAAATGC+AGG - chr4_2:1586310-1586329 Msa0583140:intron 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG - chr4_2:1586572-1586591 Msa0583140:five_prime_UTR 40.0%
TATCACACACACGTGACATA+TGG - chr4_2:1586617-1586636 Msa0583140:five_prime_UTR 40.0%
TGAGTCTTACGACAAAATCC+CGG + chr4_2:1586912-1586931 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG - chr4_2:1586961-1586980 Msa0583140:five_prime_UTR 40.0%
GCTGCAAAAGGAAGAAGCAA+TGG + chr4_2:1585651-1585670 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG - chr4_2:1586890-1586909 Msa0583140:five_prime_UTR 45.0%
! ACATGGATGATTTGTCCACC+GGG - chr4_2:1586891-1586910 Msa0583140:five_prime_UTR 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG + chr4_2:1586983-1587002 None:intergenic 45.0%
GCTGCAAAAGGAAGAAGCAA+TGG + chr4_2:1585651-1585670 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG - chr4_2:1586890-1586909 Msa0583140:five_prime_UTR 45.0%
! ACATGGATGATTTGTCCACC+GGG - chr4_2:1586891-1586910 Msa0583140:five_prime_UTR 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG + chr4_2:1586983-1587002 None:intergenic 45.0%
GTCTTACGACAAAATCCCGG+TGG + chr4_2:1586909-1586928 None:intergenic 50.0%
GTCTTACGACAAAATCCCGG+TGG + chr4_2:1586909-1586928 None:intergenic 50.0%
ACTAGCACGGATCACCGTTG+TGG - chr4_2:1586149-1586168 Msa0583140:intron 55.0%
CTAGCACGGATCACCGTTGT+GGG - chr4_2:1586150-1586169 Msa0583140:intron 55.0%
ACTAGCACGGATCACCGTTG+TGG - chr4_2:1586149-1586168 Msa0583140:intron 55.0%
CTAGCACGGATCACCGTTGT+GGG - chr4_2:1586150-1586169 Msa0583140:intron 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 1585628 1587194 1585628 ID=Msa0583140;Name=Msa0583140
chr4_2 mRNA 1585628 1587194 1585628 ID=Msa0583140-mRNA-1;Parent=Msa0583140;Name=Msa0583140-mRNA-1;_AED=0.00;_eAED=0.00;_QI=643|1|1|1|1|1|2|250|61
chr4_2 exon 1585628 1586002 1585628 ID=Msa0583140-mRNA-1:exon:412;Parent=Msa0583140-mRNA-1
chr4_2 exon 1586491 1587194 1586491 ID=Msa0583140-mRNA-1:exon:411;Parent=Msa0583140-mRNA-1
chr4_2 five_prime_UTR 1586552 1587194 1586552 ID=Msa0583140-mRNA-1:five_prime_utr;Parent=Msa0583140-mRNA-1
chr4_2 CDS 1586491 1586551 1586491 ID=Msa0583140-mRNA-1:cds;Parent=Msa0583140-mRNA-1
chr4_2 CDS 1585878 1586002 1585878 ID=Msa0583140-mRNA-1:cds;Parent=Msa0583140-mRNA-1
chr4_2 three_prime_UTR 1585628 1585877 1585628 ID=Msa0583140-mRNA-1:three_prime_utr;Parent=Msa0583140-mRNA-1
Gene Sequence

>Msa0583140

ATGGAAGTTGGAAAAAGTGGTCATTCGTGTGTGGTGACGATCGACCTTGTACAAAATGCAGGTTGTGAGATTGACATCAACTATTTACAAGTTCAATCAATTACTTTAGCTTCAACTTTGTTGATCAATGAGACATGGATGATTTGTCCACCGGGATTTTGTCGTAAGACTCACTATAAACCATAG
Protein sequence

>Msa0583140

MEVGKSGHSCVVTIDLVQNAGCEIDINYLQVQSITLASTLLINETWMICPPGFCRKTHYKP*