AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1235160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTCCAGTTTATTAATT+TGG 0.140240 8_2:+12965478 Msa1235160:five_prime_UTR
ATTCTTCAGGGTGCTGTTTC+CGG 0.182480 8_2:-12966710 None:intergenic
GAAGATTAACCGTAGTTTAA+TGG 0.206013 8_2:+12965786 Msa1235160:five_prime_UTR
ATATTTCCAGTTTAATTAAA+AGG 0.245381 8_2:-12965831 None:intergenic
ATTGATCACTCAAGGCAATT+TGG 0.255887 8_2:+12966677 Msa1235160:three_prime_UTR
CCTTGAGTGATCAATTTCTA+TGG 0.275219 8_2:-12966669 None:intergenic
TAGCAATGTTCAAATTCTTC+AGG 0.275366 8_2:-12966723 None:intergenic
TATATTCCAAATTAATAAAC+TGG 0.334256 8_2:-12965484 None:intergenic
AAATAAGTACAATGGAATTA+TGG 0.343125 8_2:+12965982 Msa1235160:exon
TACAATGGAATTATGGAAGT+TGG 0.379712 8_2:+12965989 Msa1235160:exon
CAAGGCAATTTGGACTATAA+TGG 0.384200 8_2:+12966687 Msa1235160:three_prime_UTR
CATTGCTATAATCCTATAGT+TGG 0.391238 8_2:+12966738 Msa1235160:three_prime_UTR
ATCAGTTTAAATCCAACTAT+AGG 0.412017 8_2:-12966750 None:intergenic
AACTGGAAATATTTGAGAGC+TGG 0.415904 8_2:+12965842 Msa1235160:five_prime_UTR
GGATTTAAACTGATGACCTT+AGG 0.438673 8_2:+12966759 Msa1235160:three_prime_UTR
ACTGGAAATATTTGAGAGCT+GGG 0.463256 8_2:+12965843 Msa1235160:five_prime_UTR
CAAAGAGAAGAAGCTGCAAA+AGG 0.479266 8_2:-12965390 None:intergenic
CAGTGTTTCAGTTTAACCTA+AGG 0.497072 8_2:-12966775 None:intergenic
TGTGTTCACCTTCTAAGAAA+AGG 0.512552 8_2:-12965924 None:intergenic
GACATGGATGATTTGTCCAC+CGG 0.528848 8_2:+12966620 Msa1235160:CDS
AGCAATGTTCAAATTCTTCA+GGG 0.534834 8_2:-12966722 None:intergenic
TAAAGCTGAATTATATTCTG+TGG 0.543595 8_2:-12965958 None:intergenic
CAATGGTAAATGAAGAAGAA+TGG 0.546725 8_2:-12965361 None:intergenic
CTTTGTTGATCAATGAGACA+TGG 0.564063 8_2:+12966604 Msa1235160:CDS
ATCGACCTTGTACAAAATGC+AGG 0.570197 8_2:+12966040 Msa1235160:CDS
CTAGCACGGATCACCGTTGT+GGG 0.575018 8_2:+12965880 Msa1235160:five_prime_UTR
CCATAGAAATTGATCACTCA+AGG 0.576539 8_2:+12966669 Msa1235160:exon
ACATAGAGGCCATTAAACTA+CGG 0.579332 8_2:-12965795 None:intergenic
GCAATTTGGACTATAATGGC+CGG 0.581730 8_2:+12966691 Msa1235160:three_prime_UTR
ACATGGATGATTTGTCCACC+GGG 0.590553 8_2:+12966621 Msa1235160:CDS
ACTAGCACGGATCACCGTTG+TGG 0.591441 8_2:+12965879 Msa1235160:five_prime_UTR
AGCTTTACAAATAAGTACAA+TGG 0.598879 8_2:+12965974 Msa1235160:five_prime_UTR
GATTGTATGATACGCTAGAA+CGG 0.606609 8_2:-12965439 None:intergenic
TGAGTCTTACGACAAAATCC+CGG 0.608610 8_2:-12966639 None:intergenic
GCTGCAAAAGGAAGAAGCAA+TGG 0.610138 8_2:-12965378 None:intergenic
AATTAATCAATATTAAGCTG+TGG 0.613201 8_2:-12965461 None:intergenic
GTCTTACGACAAAATCCCGG+TGG 0.729703 8_2:-12966636 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCCAGTTTATTAATT+TGG + chr8_2:12965478-12965497 Msa1235160:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_2:12965487-12965506 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_2:12965661-12965680 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_2:12965726-12965745 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_2:12965834-12965853 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_2:12966063-12966082 Msa1235160:intron 15.0%
!! TTAATTCCAGTTTATTAATT+TGG + chr8_2:12965478-12965497 Msa1235160:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_2:12965487-12965506 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_2:12965661-12965680 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_2:12965726-12965745 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_2:12965834-12965853 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_2:12966063-12966082 Msa1235160:intron 15.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_2:12965464-12965483 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_2:12965741-12965760 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_2:12965825-12965844 Msa1235160:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_2:12965982-12966001 Msa1235160:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_2:12966112-12966131 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_2:12966113-12966132 Msa1235160:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_2:12966114-12966133 Msa1235160:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_2:12966150-12966169 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_2:12966167-12966186 Msa1235160:intron 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_2:12966273-12966292 Msa1235160:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_2:12966285-12966304 Msa1235160:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_2:12966388-12966407 Msa1235160:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_2:12966389-12966408 Msa1235160:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_2:12966414-12966433 Msa1235160:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_2:12966821-12966840 None:intergenic 20.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_2:12965464-12965483 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_2:12965741-12965760 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_2:12965825-12965844 Msa1235160:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_2:12965982-12966001 Msa1235160:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_2:12966112-12966131 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_2:12966113-12966132 Msa1235160:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_2:12966114-12966133 Msa1235160:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_2:12966150-12966169 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_2:12966167-12966186 Msa1235160:intron 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_2:12966273-12966292 Msa1235160:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_2:12966285-12966304 Msa1235160:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_2:12966388-12966407 Msa1235160:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_2:12966389-12966408 Msa1235160:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_2:12966414-12966433 Msa1235160:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_2:12966821-12966840 None:intergenic 20.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_2:12965500-12965519 Msa1235160:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_2:12965526-12965545 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_2:12965694-12965713 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_2:12965721-12965740 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_2:12965812-12965831 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_2:12965866-12965885 Msa1235160:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_2:12965961-12965980 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_2:12965974-12965993 Msa1235160:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_2:12966074-12966093 Msa1235160:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_2:12966115-12966134 Msa1235160:intron 25.0%
!! CATTTTTGCGTATATGAATA+TGG - chr8_2:12966215-12966234 None:intergenic 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_2:12966753-12966772 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_2:12966806-12966825 Msa1235160:three_prime_UTR 25.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_2:12965500-12965519 Msa1235160:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_2:12965526-12965545 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_2:12965694-12965713 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_2:12965721-12965740 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_2:12965812-12965831 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_2:12965866-12965885 Msa1235160:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_2:12965961-12965980 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_2:12965974-12965993 Msa1235160:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_2:12966074-12966093 Msa1235160:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_2:12966115-12966134 Msa1235160:intron 25.0%
!! CATTTTTGCGTATATGAATA+TGG - chr8_2:12966215-12966234 None:intergenic 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_2:12966753-12966772 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_2:12966806-12966825 Msa1235160:three_prime_UTR 25.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_2:12965364-12965383 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_2:12965681-12965700 Msa1235160:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_2:12965786-12965805 Msa1235160:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_2:12965916-12965935 Msa1235160:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_2:12965989-12966008 Msa1235160:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_2:12965998-12966017 Msa1235160:exon 30.0%
! GTACATTTGGAAACCTATTT+TGG + chr8_2:12966096-12966115 Msa1235160:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_2:12966116-12966135 Msa1235160:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_2:12966431-12966450 Msa1235160:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_2:12966725-12966744 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_2:12966726-12966745 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_2:12966738-12966757 Msa1235160:three_prime_UTR 30.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_2:12965364-12965383 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_2:12965681-12965700 Msa1235160:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_2:12965786-12965805 Msa1235160:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_2:12965916-12965935 Msa1235160:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_2:12965989-12966008 Msa1235160:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_2:12965998-12966017 Msa1235160:exon 30.0%
! GTACATTTGGAAACCTATTT+TGG + chr8_2:12966096-12966115 Msa1235160:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_2:12966116-12966135 Msa1235160:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_2:12966431-12966450 Msa1235160:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_2:12966725-12966744 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_2:12966726-12966745 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_2:12966738-12966757 Msa1235160:three_prime_UTR 30.0%
GATTGTATGATACGCTAGAA+CGG - chr8_2:12965442-12965461 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_2:12965798-12965817 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_2:12965842-12965861 Msa1235160:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_2:12965843-12965862 Msa1235160:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_2:12965896-12965915 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_2:12965927-12965946 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG - chr8_2:12966048-12966067 None:intergenic 35.0%
AGGTAAAATGCAGGTACATT+TGG + chr8_2:12966083-12966102 Msa1235160:intron 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_2:12966117-12966136 Msa1235160:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_2:12966118-12966137 Msa1235160:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_2:12966314-12966333 Msa1235160:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_2:12966511-12966530 Msa1235160:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_2:12966528-12966547 Msa1235160:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_2:12966604-12966623 Msa1235160:CDS 35.0%
CCTTGAGTGATCAATTTCTA+TGG - chr8_2:12966672-12966691 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG + chr8_2:12966669-12966688 Msa1235160:exon 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_2:12966677-12966696 Msa1235160:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_2:12966687-12966706 Msa1235160:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_2:12966759-12966778 Msa1235160:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_2:12966778-12966797 None:intergenic 35.0%
GATTGTATGATACGCTAGAA+CGG - chr8_2:12965442-12965461 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_2:12965798-12965817 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_2:12965842-12965861 Msa1235160:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_2:12965843-12965862 Msa1235160:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_2:12965896-12965915 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_2:12965927-12965946 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG - chr8_2:12966048-12966067 None:intergenic 35.0%
AGGTAAAATGCAGGTACATT+TGG + chr8_2:12966083-12966102 Msa1235160:intron 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_2:12966117-12966136 Msa1235160:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_2:12966118-12966137 Msa1235160:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_2:12966314-12966333 Msa1235160:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_2:12966511-12966530 Msa1235160:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_2:12966528-12966547 Msa1235160:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_2:12966604-12966623 Msa1235160:CDS 35.0%
CCTTGAGTGATCAATTTCTA+TGG - chr8_2:12966672-12966691 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG + chr8_2:12966669-12966688 Msa1235160:exon 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_2:12966677-12966696 Msa1235160:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_2:12966687-12966706 Msa1235160:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_2:12966759-12966778 Msa1235160:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_2:12966778-12966797 None:intergenic 35.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_2:12965393-12965412 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_2:12965391-12965410 Msa1235160:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_2:12965506-12965525 Msa1235160:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_2:12966012-12966031 Msa1235160:CDS 40.0%
ATCGACCTTGTACAAAATGC+AGG + chr8_2:12966040-12966059 Msa1235160:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_2:12966302-12966321 Msa1235160:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_2:12966347-12966366 Msa1235160:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_2:12966642-12966661 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_2:12966691-12966710 Msa1235160:three_prime_UTR 40.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_2:12965393-12965412 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_2:12965391-12965410 Msa1235160:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_2:12965506-12965525 Msa1235160:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_2:12966012-12966031 Msa1235160:CDS 40.0%
ATCGACCTTGTACAAAATGC+AGG + chr8_2:12966040-12966059 Msa1235160:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_2:12966302-12966321 Msa1235160:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_2:12966347-12966366 Msa1235160:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_2:12966642-12966661 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_2:12966691-12966710 Msa1235160:three_prime_UTR 40.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_2:12965381-12965400 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_2:12966620-12966639 Msa1235160:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_2:12966621-12966640 Msa1235160:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_2:12966713-12966732 None:intergenic 45.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_2:12965381-12965400 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_2:12966620-12966639 Msa1235160:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_2:12966621-12966640 Msa1235160:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_2:12966713-12966732 None:intergenic 45.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_2:12966639-12966658 None:intergenic 50.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_2:12966639-12966658 None:intergenic 50.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_2:12965879-12965898 Msa1235160:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_2:12965880-12965899 Msa1235160:five_prime_UTR 55.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_2:12965879-12965898 Msa1235160:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_2:12965880-12965899 Msa1235160:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 12965358 12966924 12965358 ID=Msa1235160;Name=Msa1235160
chr8_2 mRNA 12965358 12966924 12965358 ID=Msa1235160-mRNA-1;Parent=Msa1235160;Name=Msa1235160-mRNA-1;_AED=0.00;_eAED=0.00;_QI=643|1|1|1|1|1|2|250|61
chr8_2 exon 12965358 12966061 12965358 ID=Msa1235160-mRNA-1:exon:5017;Parent=Msa1235160-mRNA-1
chr8_2 exon 12966550 12966924 12966550 ID=Msa1235160-mRNA-1:exon:5018;Parent=Msa1235160-mRNA-1
chr8_2 five_prime_UTR 12965358 12966000 12965358 ID=Msa1235160-mRNA-1:five_prime_utr;Parent=Msa1235160-mRNA-1
chr8_2 CDS 12966001 12966061 12966001 ID=Msa1235160-mRNA-1:cds;Parent=Msa1235160-mRNA-1
chr8_2 CDS 12966550 12966674 12966550 ID=Msa1235160-mRNA-1:cds;Parent=Msa1235160-mRNA-1
chr8_2 three_prime_UTR 12966675 12966924 12966675 ID=Msa1235160-mRNA-1:three_prime_utr;Parent=Msa1235160-mRNA-1
Gene Sequence

>Msa1235160

ATGGAAGTTGGAAAAAGTGGTCATTCGTGTGTGGTGACGATCGACCTTGTACAAAATGCAGGTTGTGAGATTGACATCAACTATTTACAAGTTCAATCAATTACTTTAGCTTCAACTTTGTTGATCAATGAGACATGGATGATTTGTCCACCGGGATTTTGTCGTAAGACTCACTATAAACCATAG
Protein sequence

>Msa1235160

MEVGKSGHSCVVTIDLVQNAGCEIDINYLQVQSITLASTLLINETWMICPPGFCRKTHYKP*