AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1272230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTCCAGTTTATTAATT+TGG 0.140240 8_3:+5734298 Msa1272230:five_prime_UTR
ATTCTTCAGGGTGCTGTTTC+CGG 0.182480 8_3:-5735530 None:intergenic
GAAGATTAACCGTAGTTTAA+TGG 0.206013 8_3:+5734606 Msa1272230:five_prime_UTR
ATATTTCCAGTTTAATTAAA+AGG 0.245381 8_3:-5734651 None:intergenic
ATTGATCACTCAAGGCAATT+TGG 0.255887 8_3:+5735497 Msa1272230:three_prime_UTR
CCTTGAGTGATCAATTTCTA+TGG 0.275219 8_3:-5735489 None:intergenic
TAGCAATGTTCAAATTCTTC+AGG 0.275366 8_3:-5735543 None:intergenic
TATATTCCAAATTAATAAAC+TGG 0.334256 8_3:-5734304 None:intergenic
AAATAAGTACAATGGAATTA+TGG 0.343125 8_3:+5734802 Msa1272230:exon
TACAATGGAATTATGGAAGT+TGG 0.379712 8_3:+5734809 Msa1272230:exon
CAAGGCAATTTGGACTATAA+TGG 0.384200 8_3:+5735507 Msa1272230:three_prime_UTR
CATTGCTATAATCCTATAGT+TGG 0.391238 8_3:+5735558 Msa1272230:three_prime_UTR
ATCAGTTTAAATCCAACTAT+AGG 0.412017 8_3:-5735570 None:intergenic
AACTGGAAATATTTGAGAGC+TGG 0.415904 8_3:+5734662 Msa1272230:five_prime_UTR
GGATTTAAACTGATGACCTT+AGG 0.438673 8_3:+5735579 Msa1272230:three_prime_UTR
ACTGGAAATATTTGAGAGCT+GGG 0.463256 8_3:+5734663 Msa1272230:five_prime_UTR
CAAAGAGAAGAAGCTGCAAA+AGG 0.479266 8_3:-5734210 None:intergenic
CAGTGTTTCAGTTTAACCTA+AGG 0.497072 8_3:-5735595 None:intergenic
TGTGTTCACCTTCTAAGAAA+AGG 0.512552 8_3:-5734744 None:intergenic
GACATGGATGATTTGTCCAC+CGG 0.528848 8_3:+5735440 Msa1272230:CDS
AGCAATGTTCAAATTCTTCA+GGG 0.534834 8_3:-5735542 None:intergenic
TAAAGCTGAATTATATTCTG+TGG 0.543595 8_3:-5734778 None:intergenic
CAATGGTAAATGAAGAAGAA+TGG 0.546725 8_3:-5734181 None:intergenic
CTTTGTTGATCAATGAGACA+TGG 0.564063 8_3:+5735424 Msa1272230:CDS
ATCGACCTTGTACAAAATGC+AGG 0.570197 8_3:+5734860 Msa1272230:CDS
CTAGCACGGATCACCGTTGT+GGG 0.575018 8_3:+5734700 Msa1272230:five_prime_UTR
CCATAGAAATTGATCACTCA+AGG 0.576539 8_3:+5735489 Msa1272230:exon
ACATAGAGGCCATTAAACTA+CGG 0.579332 8_3:-5734615 None:intergenic
GCAATTTGGACTATAATGGC+CGG 0.581730 8_3:+5735511 Msa1272230:three_prime_UTR
ACATGGATGATTTGTCCACC+GGG 0.590553 8_3:+5735441 Msa1272230:CDS
ACTAGCACGGATCACCGTTG+TGG 0.591441 8_3:+5734699 Msa1272230:five_prime_UTR
AGCTTTACAAATAAGTACAA+TGG 0.598879 8_3:+5734794 Msa1272230:five_prime_UTR
GATTGTATGATACGCTAGAA+CGG 0.606609 8_3:-5734259 None:intergenic
TGAGTCTTACGACAAAATCC+CGG 0.608610 8_3:-5735459 None:intergenic
GCTGCAAAAGGAAGAAGCAA+TGG 0.610138 8_3:-5734198 None:intergenic
AATTAATCAATATTAAGCTG+TGG 0.613201 8_3:-5734281 None:intergenic
GTCTTACGACAAAATCCCGG+TGG 0.729703 8_3:-5735456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCCAGTTTATTAATT+TGG + chr8_3:5734298-5734317 Msa1272230:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_3:5734307-5734326 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_3:5734481-5734500 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_3:5734546-5734565 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_3:5734654-5734673 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_3:5734883-5734902 Msa1272230:intron 15.0%
!! TTAATTCCAGTTTATTAATT+TGG + chr8_3:5734298-5734317 Msa1272230:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_3:5734307-5734326 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_3:5734481-5734500 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_3:5734546-5734565 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_3:5734654-5734673 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_3:5734883-5734902 Msa1272230:intron 15.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_3:5734284-5734303 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_3:5734561-5734580 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_3:5734645-5734664 Msa1272230:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_3:5734802-5734821 Msa1272230:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_3:5734932-5734951 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_3:5734933-5734952 Msa1272230:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_3:5734934-5734953 Msa1272230:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_3:5734970-5734989 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_3:5734987-5735006 Msa1272230:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG - chr8_3:5735035-5735054 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_3:5735093-5735112 Msa1272230:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_3:5735105-5735124 Msa1272230:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_3:5735208-5735227 Msa1272230:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_3:5735209-5735228 Msa1272230:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_3:5735234-5735253 Msa1272230:intron 20.0%
!!! TTTTCTTTTCATTGATTTCA+TGG + chr8_3:5735276-5735295 Msa1272230:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_3:5735641-5735660 None:intergenic 20.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_3:5734284-5734303 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_3:5734561-5734580 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_3:5734645-5734664 Msa1272230:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_3:5734802-5734821 Msa1272230:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_3:5734932-5734951 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_3:5734933-5734952 Msa1272230:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_3:5734934-5734953 Msa1272230:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_3:5734970-5734989 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_3:5734987-5735006 Msa1272230:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG - chr8_3:5735035-5735054 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_3:5735093-5735112 Msa1272230:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_3:5735105-5735124 Msa1272230:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_3:5735208-5735227 Msa1272230:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_3:5735209-5735228 Msa1272230:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_3:5735234-5735253 Msa1272230:intron 20.0%
!!! TTTTCTTTTCATTGATTTCA+TGG + chr8_3:5735276-5735295 Msa1272230:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_3:5735641-5735660 None:intergenic 20.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_3:5734320-5734339 Msa1272230:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_3:5734346-5734365 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_3:5734514-5734533 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_3:5734541-5734560 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_3:5734632-5734651 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_3:5734686-5734705 Msa1272230:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_3:5734781-5734800 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_3:5734794-5734813 Msa1272230:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_3:5734894-5734913 Msa1272230:intron 25.0%
!!! GTATTTTTGGAAACCTATTT+TGG + chr8_3:5734916-5734935 Msa1272230:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_3:5734935-5734954 Msa1272230:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_3:5735573-5735592 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_3:5735626-5735645 Msa1272230:three_prime_UTR 25.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_3:5734320-5734339 Msa1272230:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_3:5734346-5734365 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_3:5734514-5734533 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_3:5734541-5734560 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_3:5734632-5734651 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_3:5734686-5734705 Msa1272230:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_3:5734781-5734800 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_3:5734794-5734813 Msa1272230:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_3:5734894-5734913 Msa1272230:intron 25.0%
!!! GTATTTTTGGAAACCTATTT+TGG + chr8_3:5734916-5734935 Msa1272230:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_3:5734935-5734954 Msa1272230:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_3:5735573-5735592 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_3:5735626-5735645 Msa1272230:three_prime_UTR 25.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_3:5734184-5734203 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_3:5734501-5734520 Msa1272230:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_3:5734606-5734625 Msa1272230:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_3:5734736-5734755 Msa1272230:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_3:5734809-5734828 Msa1272230:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_3:5734818-5734837 Msa1272230:exon 30.0%
! AGGTAAAATGCAGGTATTTT+TGG + chr8_3:5734903-5734922 Msa1272230:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_3:5734936-5734955 Msa1272230:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_3:5735251-5735270 Msa1272230:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_3:5735545-5735564 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_3:5735546-5735565 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_3:5735558-5735577 Msa1272230:three_prime_UTR 30.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_3:5734184-5734203 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_3:5734501-5734520 Msa1272230:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_3:5734606-5734625 Msa1272230:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_3:5734736-5734755 Msa1272230:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_3:5734809-5734828 Msa1272230:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_3:5734818-5734837 Msa1272230:exon 30.0%
! AGGTAAAATGCAGGTATTTT+TGG + chr8_3:5734903-5734922 Msa1272230:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_3:5734936-5734955 Msa1272230:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_3:5735251-5735270 Msa1272230:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_3:5735545-5735564 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_3:5735546-5735565 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_3:5735558-5735577 Msa1272230:three_prime_UTR 30.0%
GATTGTATGATACGCTAGAA+CGG - chr8_3:5734262-5734281 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_3:5734618-5734637 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_3:5734662-5734681 Msa1272230:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_3:5734663-5734682 Msa1272230:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_3:5734716-5734735 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_3:5734747-5734766 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG - chr8_3:5734868-5734887 None:intergenic 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_3:5734937-5734956 Msa1272230:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_3:5734938-5734957 Msa1272230:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_3:5735134-5735153 Msa1272230:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_3:5735331-5735350 Msa1272230:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_3:5735348-5735367 Msa1272230:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_3:5735424-5735443 Msa1272230:CDS 35.0%
CCTTGAGTGATCAATTTCTA+TGG - chr8_3:5735492-5735511 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG + chr8_3:5735489-5735508 Msa1272230:exon 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_3:5735497-5735516 Msa1272230:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_3:5735507-5735526 Msa1272230:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_3:5735579-5735598 Msa1272230:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_3:5735598-5735617 None:intergenic 35.0%
GATTGTATGATACGCTAGAA+CGG - chr8_3:5734262-5734281 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_3:5734618-5734637 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_3:5734662-5734681 Msa1272230:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_3:5734663-5734682 Msa1272230:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_3:5734716-5734735 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_3:5734747-5734766 None:intergenic 35.0%
! ATGTACCTGCATTTTGTACA+AGG - chr8_3:5734868-5734887 None:intergenic 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_3:5734937-5734956 Msa1272230:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_3:5734938-5734957 Msa1272230:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_3:5735134-5735153 Msa1272230:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_3:5735331-5735350 Msa1272230:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_3:5735348-5735367 Msa1272230:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_3:5735424-5735443 Msa1272230:CDS 35.0%
CCTTGAGTGATCAATTTCTA+TGG - chr8_3:5735492-5735511 None:intergenic 35.0%
CCATAGAAATTGATCACTCA+AGG + chr8_3:5735489-5735508 Msa1272230:exon 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_3:5735497-5735516 Msa1272230:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_3:5735507-5735526 Msa1272230:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_3:5735579-5735598 Msa1272230:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_3:5735598-5735617 None:intergenic 35.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_3:5734213-5734232 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_3:5734211-5734230 Msa1272230:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_3:5734326-5734345 Msa1272230:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_3:5734832-5734851 Msa1272230:CDS 40.0%
ATCGACCTTGTACAAAATGC+AGG + chr8_3:5734860-5734879 Msa1272230:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_3:5735122-5735141 Msa1272230:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_3:5735167-5735186 Msa1272230:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_3:5735462-5735481 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_3:5735511-5735530 Msa1272230:three_prime_UTR 40.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_3:5734213-5734232 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_3:5734211-5734230 Msa1272230:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_3:5734326-5734345 Msa1272230:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_3:5734832-5734851 Msa1272230:CDS 40.0%
ATCGACCTTGTACAAAATGC+AGG + chr8_3:5734860-5734879 Msa1272230:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_3:5735122-5735141 Msa1272230:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_3:5735167-5735186 Msa1272230:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_3:5735462-5735481 None:intergenic 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_3:5735511-5735530 Msa1272230:three_prime_UTR 40.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_3:5734201-5734220 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_3:5735440-5735459 Msa1272230:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_3:5735441-5735460 Msa1272230:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_3:5735533-5735552 None:intergenic 45.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_3:5734201-5734220 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_3:5735440-5735459 Msa1272230:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_3:5735441-5735460 Msa1272230:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_3:5735533-5735552 None:intergenic 45.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_3:5735459-5735478 None:intergenic 50.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_3:5735459-5735478 None:intergenic 50.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_3:5734699-5734718 Msa1272230:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_3:5734700-5734719 Msa1272230:five_prime_UTR 55.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_3:5734699-5734718 Msa1272230:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_3:5734700-5734719 Msa1272230:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr8_3 gene 5734178 5735744 5734178 ID=Msa1272230;Name=Msa1272230
chr8_3 mRNA 5734178 5735744 5734178 ID=Msa1272230-mRNA-1;Parent=Msa1272230;Name=Msa1272230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=643|1|1|1|1|1|2|250|61
chr8_3 exon 5734178 5734881 5734178 ID=Msa1272230-mRNA-1:exon:2055;Parent=Msa1272230-mRNA-1
chr8_3 exon 5735370 5735744 5735370 ID=Msa1272230-mRNA-1:exon:2056;Parent=Msa1272230-mRNA-1
chr8_3 five_prime_UTR 5734178 5734820 5734178 ID=Msa1272230-mRNA-1:five_prime_utr;Parent=Msa1272230-mRNA-1
chr8_3 CDS 5734821 5734881 5734821 ID=Msa1272230-mRNA-1:cds;Parent=Msa1272230-mRNA-1
chr8_3 CDS 5735370 5735494 5735370 ID=Msa1272230-mRNA-1:cds;Parent=Msa1272230-mRNA-1
chr8_3 three_prime_UTR 5735495 5735744 5735495 ID=Msa1272230-mRNA-1:three_prime_utr;Parent=Msa1272230-mRNA-1
Gene Sequence

>Msa1272230

ATGGAAGTTGGAAAAAGTGGTCATTCGTGTGTGGTGACGATCGACCTTGTACAAAATGCAGGTTGTGAGATTGACATCAACTATTTACAAGTTCAATCAATTACTTTAGCTTCAACTTTGTTGATCAATGAGACATGGATGATTTGTCCACCGGGATTTTGTCGTAAGACTCACTATAAACCATAG
Protein sequence

>Msa1272230

MEVGKSGHSCVVTIDLVQNAGCEIDINYLQVQSITLASTLLINETWMICPPGFCRKTHYKP*