AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1309980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 37 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTCCAGTTTATTAATT+TGG 0.140240 8_4:+6527346 Msa1309980:five_prime_UTR
ATTCTTCAGGGTGCTGTTTC+CGG 0.182480 8_4:-6528578 None:intergenic
GAAGATTAACCGTAGTTTAA+TGG 0.215491 8_4:+6527654 Msa1309980:five_prime_UTR
ATATTTCCAGTTTAATTAAA+AGG 0.245381 8_4:-6527699 None:intergenic
ATTGATCACTCAAGGCAATT+TGG 0.255887 8_4:+6528545 Msa1309980:three_prime_UTR
TAGCAATGTTCAAATTCTTC+AGG 0.275366 8_4:-6528591 None:intergenic
TATATTCCAAATTAATAAAC+TGG 0.334256 8_4:-6527352 None:intergenic
AAATAAGTACAATGGAATTA+TGG 0.343125 8_4:+6527850 Msa1309980:exon
TACAATGGAATTATGGAAGT+TGG 0.379712 8_4:+6527857 Msa1309980:exon
CAAGGCAATTTGGACTATAA+TGG 0.384200 8_4:+6528555 Msa1309980:three_prime_UTR
CATTGCTATAATCCTATAGT+TGG 0.391238 8_4:+6528606 Msa1309980:three_prime_UTR
ATCAGTTTAAATCCAACTAT+AGG 0.412017 8_4:-6528618 None:intergenic
AACTGGAAATATTTGAGAGC+TGG 0.415904 8_4:+6527710 Msa1309980:five_prime_UTR
GGATTTAAACTGATGACCTT+AGG 0.438673 8_4:+6528627 Msa1309980:three_prime_UTR
ACTGGAAATATTTGAGAGCT+GGG 0.463256 8_4:+6527711 Msa1309980:five_prime_UTR
CAAAGAGAAGAAGCTGCAAA+AGG 0.479266 8_4:-6527258 None:intergenic
CAGTGTTTCAGTTTAACCTA+AGG 0.497072 8_4:-6528643 None:intergenic
TGTGTTCACCTTCTAAGAAA+AGG 0.512552 8_4:-6527792 None:intergenic
CCTTGAGTGATCAATTTCTA+CGG 0.513394 8_4:-6528537 None:intergenic
GACATGGATGATTTGTCCAC+CGG 0.528848 8_4:+6528488 Msa1309980:CDS
AGCAATGTTCAAATTCTTCA+GGG 0.534834 8_4:-6528590 None:intergenic
TAAAGCTGAATTATATTCTG+TGG 0.543595 8_4:-6527826 None:intergenic
CAATGGTAAATGAAGAAGAA+TGG 0.546725 8_4:-6527229 None:intergenic
ATCGATCTTGTACAAAATGC+AGG 0.553196 8_4:+6527908 Msa1309980:CDS
CCGTAGAAATTGATCACTCA+AGG 0.563073 8_4:+6528537 Msa1309980:exon
CTTTGTTGATCAATGAGACA+TGG 0.564063 8_4:+6528472 Msa1309980:CDS
CTAGCACGGATCACCGTTGT+GGG 0.575018 8_4:+6527748 Msa1309980:five_prime_UTR
ACATAGAGGCCATTAAACTA+CGG 0.579332 8_4:-6527663 None:intergenic
GCAATTTGGACTATAATGGC+CGG 0.581730 8_4:+6528559 Msa1309980:three_prime_UTR
ACATGGATGATTTGTCCACC+GGG 0.590553 8_4:+6528489 Msa1309980:CDS
ACTAGCACGGATCACCGTTG+TGG 0.591441 8_4:+6527747 Msa1309980:five_prime_UTR
AGCTTTACAAATAAGTACAA+TGG 0.598879 8_4:+6527842 Msa1309980:five_prime_UTR
GATTGTATGATACGCTAGAA+CGG 0.606609 8_4:-6527307 None:intergenic
TGAGTCTTACGACAAAATCC+CGG 0.608610 8_4:-6528507 None:intergenic
GCTGCAAAAGGAAGAAGCAA+TGG 0.610138 8_4:-6527246 None:intergenic
AATTAATCAATATTAAGCTG+TGG 0.613201 8_4:-6527329 None:intergenic
GTCTTACGACAAAATCCCGG+TGG 0.729703 8_4:-6528504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCCAGTTTATTAATT+TGG + chr8_4:6527346-6527365 Msa1309980:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_4:6527355-6527374 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_4:6527529-6527548 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_4:6527594-6527613 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_4:6527702-6527721 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_4:6527931-6527950 Msa1309980:intron 15.0%
!! TTAATTCCAGTTTATTAATT+TGG + chr8_4:6527346-6527365 Msa1309980:five_prime_UTR 15.0%
!! TATATTCCAAATTAATAAAC+TGG - chr8_4:6527355-6527374 None:intergenic 15.0%
!!! TAAGAATTTGATATTTTGTA+AGG - chr8_4:6527529-6527548 None:intergenic 15.0%
!! AAAAATGGAAGAAAAAAAAA+TGG - chr8_4:6527594-6527613 None:intergenic 15.0%
!! ATATTTCCAGTTTAATTAAA+AGG - chr8_4:6527702-6527721 None:intergenic 15.0%
!!! TACATTTGTTTTTATTTTGA+AGG + chr8_4:6527931-6527950 Msa1309980:intron 15.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_4:6527332-6527351 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_4:6527609-6527628 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_4:6527693-6527712 Msa1309980:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_4:6527850-6527869 Msa1309980:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_4:6527980-6527999 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_4:6527981-6528000 Msa1309980:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_4:6527982-6528001 Msa1309980:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_4:6528018-6528037 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_4:6528035-6528054 Msa1309980:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG - chr8_4:6528083-6528102 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_4:6528141-6528160 Msa1309980:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_4:6528153-6528172 Msa1309980:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_4:6528256-6528275 Msa1309980:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_4:6528257-6528276 Msa1309980:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_4:6528282-6528301 Msa1309980:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_4:6528689-6528708 None:intergenic 20.0%
!! AATTAATCAATATTAAGCTG+TGG - chr8_4:6527332-6527351 None:intergenic 20.0%
!! TTATATGTCTCAACAAAAAA+TGG - chr8_4:6527609-6527628 None:intergenic 20.0%
!!! TTATGTCCTTTTAATTAAAC+TGG + chr8_4:6527693-6527712 Msa1309980:five_prime_UTR 20.0%
!! AAATAAGTACAATGGAATTA+TGG + chr8_4:6527850-6527869 Msa1309980:exon 20.0%
!! AAATATACAGTATCCAAAAT+AGG - chr8_4:6527980-6527999 None:intergenic 20.0%
!!! TTTTGGATACTGTATATTTT+TGG + chr8_4:6527981-6528000 Msa1309980:intron 20.0%
!!! TTTGGATACTGTATATTTTT+GGG + chr8_4:6527982-6528001 Msa1309980:intron 20.0%
!! TAACGTTAAATGAAATACTA+CGG - chr8_4:6528018-6528037 None:intergenic 20.0%
!! TTAAGTTTAAGAAAGACAAA+AGG + chr8_4:6528035-6528054 Msa1309980:intron 20.0%
!!! TATTTTTGCGTATATGAATA+TGG - chr8_4:6528083-6528102 None:intergenic 20.0%
!! ATGAAATACATGAAACATAT+TGG + chr8_4:6528141-6528160 Msa1309980:intron 20.0%
!!! AAACATATTGGTGTTAAATT+TGG + chr8_4:6528153-6528172 Msa1309980:intron 20.0%
!! TGAATTATTATAACTACACT+TGG + chr8_4:6528256-6528275 Msa1309980:intron 20.0%
!! GAATTATTATAACTACACTT+GGG + chr8_4:6528257-6528276 Msa1309980:intron 20.0%
!!! AATTTGTGTATTTTTCTAAG+TGG + chr8_4:6528282-6528301 Msa1309980:intron 20.0%
!! TAAAAGAAAAATCCTTCTAA+TGG - chr8_4:6528689-6528708 None:intergenic 20.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_4:6527368-6527387 Msa1309980:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_4:6527394-6527413 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_4:6527562-6527581 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_4:6527589-6527608 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_4:6527680-6527699 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_4:6527734-6527753 Msa1309980:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_4:6527829-6527848 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_4:6527842-6527861 Msa1309980:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_4:6527942-6527961 Msa1309980:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_4:6527983-6528002 Msa1309980:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_4:6528621-6528640 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_4:6528674-6528693 Msa1309980:three_prime_UTR 25.0%
!!! GAATATAGATGTTTTGATAG+AGG + chr8_4:6527368-6527387 Msa1309980:five_prime_UTR 25.0%
! AACAAGAAAAATAATGACCT+AGG - chr8_4:6527394-6527413 None:intergenic 25.0%
! GATATAACTACCAAAAAAGA+AGG - chr8_4:6527562-6527581 None:intergenic 25.0%
! TGGAAGAAAAAAAAATGGTA+AGG - chr8_4:6527589-6527608 None:intergenic 25.0%
! GACATAAAAATTTCACATAG+AGG - chr8_4:6527680-6527699 None:intergenic 25.0%
!!! ATATTTTTGATACACTAGCA+CGG + chr8_4:6527734-6527753 Msa1309980:five_prime_UTR 25.0%
! TAAAGCTGAATTATATTCTG+TGG - chr8_4:6527829-6527848 None:intergenic 25.0%
! AGCTTTACAAATAAGTACAA+TGG + chr8_4:6527842-6527861 Msa1309980:five_prime_UTR 25.0%
!!! TTATTTTGAAGGTAAAATGC+AGG + chr8_4:6527942-6527961 Msa1309980:intron 25.0%
!!! TTGGATACTGTATATTTTTG+GGG + chr8_4:6527983-6528002 Msa1309980:intron 25.0%
! ATCAGTTTAAATCCAACTAT+AGG - chr8_4:6528621-6528640 None:intergenic 25.0%
!! TCAATTTTGTATCCATTAGA+AGG + chr8_4:6528674-6528693 Msa1309980:three_prime_UTR 25.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_4:6527232-6527251 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_4:6527549-6527568 Msa1309980:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_4:6527654-6527673 Msa1309980:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_4:6527784-6527803 Msa1309980:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_4:6527857-6527876 Msa1309980:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_4:6527866-6527885 Msa1309980:exon 30.0%
! GTACATTTGGAAACCTATTT+TGG + chr8_4:6527964-6527983 Msa1309980:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_4:6527984-6528003 Msa1309980:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_4:6528299-6528318 Msa1309980:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_4:6528593-6528612 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_4:6528594-6528613 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_4:6528606-6528625 Msa1309980:three_prime_UTR 30.0%
CAATGGTAAATGAAGAAGAA+TGG - chr8_4:6527232-6527251 None:intergenic 30.0%
! CGTTCTTTTTCCTTCTTTTT+TGG + chr8_4:6527549-6527568 Msa1309980:five_prime_UTR 30.0%
GAAGATTAACCGTAGTTTAA+TGG + chr8_4:6527654-6527673 Msa1309980:five_prime_UTR 30.0%
! AATTGTGTCCTTTTCTTAGA+AGG + chr8_4:6527784-6527803 Msa1309980:five_prime_UTR 30.0%
TACAATGGAATTATGGAAGT+TGG + chr8_4:6527857-6527876 Msa1309980:exon 30.0%
ATTATGGAAGTTGGAAAAAG+TGG + chr8_4:6527866-6527885 Msa1309980:exon 30.0%
! GTACATTTGGAAACCTATTT+TGG + chr8_4:6527964-6527983 Msa1309980:intron 30.0%
!!! TGGATACTGTATATTTTTGG+GGG + chr8_4:6527984-6528003 Msa1309980:intron 30.0%
AAGTGGTTTCTATGTAGATT+TGG + chr8_4:6528299-6528318 Msa1309980:intron 30.0%
AGCAATGTTCAAATTCTTCA+GGG - chr8_4:6528593-6528612 None:intergenic 30.0%
TAGCAATGTTCAAATTCTTC+AGG - chr8_4:6528594-6528613 None:intergenic 30.0%
CATTGCTATAATCCTATAGT+TGG + chr8_4:6528606-6528625 Msa1309980:three_prime_UTR 30.0%
GATTGTATGATACGCTAGAA+CGG - chr8_4:6527310-6527329 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_4:6527666-6527685 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_4:6527710-6527729 Msa1309980:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_4:6527711-6527730 Msa1309980:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_4:6527764-6527783 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_4:6527795-6527814 None:intergenic 35.0%
ATCGATCTTGTACAAAATGC+AGG + chr8_4:6527908-6527927 Msa1309980:CDS 35.0%
AGGTAAAATGCAGGTACATT+TGG + chr8_4:6527951-6527970 Msa1309980:intron 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_4:6527985-6528004 Msa1309980:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_4:6527986-6528005 Msa1309980:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_4:6528182-6528201 Msa1309980:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_4:6528379-6528398 Msa1309980:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_4:6528396-6528415 Msa1309980:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_4:6528472-6528491 Msa1309980:CDS 35.0%
CCTTGAGTGATCAATTTCTA+CGG - chr8_4:6528540-6528559 None:intergenic 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_4:6528545-6528564 Msa1309980:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_4:6528555-6528574 Msa1309980:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_4:6528627-6528646 Msa1309980:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_4:6528646-6528665 None:intergenic 35.0%
GATTGTATGATACGCTAGAA+CGG - chr8_4:6527310-6527329 None:intergenic 35.0%
ACATAGAGGCCATTAAACTA+CGG - chr8_4:6527666-6527685 None:intergenic 35.0%
AACTGGAAATATTTGAGAGC+TGG + chr8_4:6527710-6527729 Msa1309980:five_prime_UTR 35.0%
ACTGGAAATATTTGAGAGCT+GGG + chr8_4:6527711-6527730 Msa1309980:five_prime_UTR 35.0%
!!! ATTTTTTACGTGACCCACAA+CGG - chr8_4:6527764-6527783 None:intergenic 35.0%
! TGTGTTCACCTTCTAAGAAA+AGG - chr8_4:6527795-6527814 None:intergenic 35.0%
ATCGATCTTGTACAAAATGC+AGG + chr8_4:6527908-6527927 Msa1309980:CDS 35.0%
AGGTAAAATGCAGGTACATT+TGG + chr8_4:6527951-6527970 Msa1309980:intron 35.0%
!!! GGATACTGTATATTTTTGGG+GGG + chr8_4:6527985-6528004 Msa1309980:intron 35.0%
!!! GATACTGTATATTTTTGGGG+GGG + chr8_4:6527986-6528005 Msa1309980:intron 35.0%
TTTGTCAGTGGAATAAGAGT+TGG + chr8_4:6528182-6528201 Msa1309980:intron 35.0%
! TTGATTCTTTTCGTTTGTCG+TGG + chr8_4:6528379-6528398 Msa1309980:intron 35.0%
TCGTGGTGTTTATTTGTTTC+AGG + chr8_4:6528396-6528415 Msa1309980:intron 35.0%
CTTTGTTGATCAATGAGACA+TGG + chr8_4:6528472-6528491 Msa1309980:CDS 35.0%
CCTTGAGTGATCAATTTCTA+CGG - chr8_4:6528540-6528559 None:intergenic 35.0%
ATTGATCACTCAAGGCAATT+TGG + chr8_4:6528545-6528564 Msa1309980:three_prime_UTR 35.0%
CAAGGCAATTTGGACTATAA+TGG + chr8_4:6528555-6528574 Msa1309980:three_prime_UTR 35.0%
GGATTTAAACTGATGACCTT+AGG + chr8_4:6528627-6528646 Msa1309980:three_prime_UTR 35.0%
CAGTGTTTCAGTTTAACCTA+AGG - chr8_4:6528646-6528665 None:intergenic 35.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_4:6527261-6527280 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_4:6527259-6527278 Msa1309980:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_4:6527374-6527393 Msa1309980:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_4:6527880-6527899 Msa1309980:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_4:6528170-6528189 Msa1309980:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_4:6528215-6528234 Msa1309980:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_4:6528510-6528529 None:intergenic 40.0%
CCGTAGAAATTGATCACTCA+AGG + chr8_4:6528537-6528556 Msa1309980:exon 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_4:6528559-6528578 Msa1309980:three_prime_UTR 40.0%
CAAAGAGAAGAAGCTGCAAA+AGG - chr8_4:6527261-6527280 None:intergenic 40.0%
! CTTTTGCAGCTTCTTCTCTT+TGG + chr8_4:6527259-6527278 Msa1309980:five_prime_UTR 40.0%
!!! AGATGTTTTGATAGAGGCCT+AGG + chr8_4:6527374-6527393 Msa1309980:five_prime_UTR 40.0%
AAAAAGTGGTCATTCGTGTG+TGG + chr8_4:6527880-6527899 Msa1309980:CDS 40.0%
! ATTTGGTCACGTTTTGTCAG+TGG + chr8_4:6528170-6528189 Msa1309980:intron 40.0%
TATCACACACACGTGACATA+TGG + chr8_4:6528215-6528234 Msa1309980:intron 40.0%
TGAGTCTTACGACAAAATCC+CGG - chr8_4:6528510-6528529 None:intergenic 40.0%
CCGTAGAAATTGATCACTCA+AGG + chr8_4:6528537-6528556 Msa1309980:exon 40.0%
GCAATTTGGACTATAATGGC+CGG + chr8_4:6528559-6528578 Msa1309980:three_prime_UTR 40.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_4:6527249-6527268 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_4:6528488-6528507 Msa1309980:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_4:6528489-6528508 Msa1309980:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_4:6528581-6528600 None:intergenic 45.0%
GCTGCAAAAGGAAGAAGCAA+TGG - chr8_4:6527249-6527268 None:intergenic 45.0%
! GACATGGATGATTTGTCCAC+CGG + chr8_4:6528488-6528507 Msa1309980:CDS 45.0%
! ACATGGATGATTTGTCCACC+GGG + chr8_4:6528489-6528508 Msa1309980:CDS 45.0%
!! ATTCTTCAGGGTGCTGTTTC+CGG - chr8_4:6528581-6528600 None:intergenic 45.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_4:6528507-6528526 None:intergenic 50.0%
GTCTTACGACAAAATCCCGG+TGG - chr8_4:6528507-6528526 None:intergenic 50.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_4:6527747-6527766 Msa1309980:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_4:6527748-6527767 Msa1309980:five_prime_UTR 55.0%
ACTAGCACGGATCACCGTTG+TGG + chr8_4:6527747-6527766 Msa1309980:five_prime_UTR 55.0%
CTAGCACGGATCACCGTTGT+GGG + chr8_4:6527748-6527767 Msa1309980:five_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 6527226 6528792 6527226 ID=Msa1309980;Name=Msa1309980
chr8_4 mRNA 6527226 6528792 6527226 ID=Msa1309980-mRNA-1;Parent=Msa1309980;Name=Msa1309980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=643|1|1|1|1|1|2|250|61
chr8_4 exon 6527226 6527929 6527226 ID=Msa1309980-mRNA-1:exon:2201;Parent=Msa1309980-mRNA-1
chr8_4 exon 6528418 6528792 6528418 ID=Msa1309980-mRNA-1:exon:2202;Parent=Msa1309980-mRNA-1
chr8_4 five_prime_UTR 6527226 6527868 6527226 ID=Msa1309980-mRNA-1:five_prime_utr;Parent=Msa1309980-mRNA-1
chr8_4 CDS 6527869 6527929 6527869 ID=Msa1309980-mRNA-1:cds;Parent=Msa1309980-mRNA-1
chr8_4 CDS 6528418 6528542 6528418 ID=Msa1309980-mRNA-1:cds;Parent=Msa1309980-mRNA-1
chr8_4 three_prime_UTR 6528543 6528792 6528543 ID=Msa1309980-mRNA-1:three_prime_utr;Parent=Msa1309980-mRNA-1
Gene Sequence

>Msa1309980

ATGGAAGTTGGAAAAAGTGGTCATTCGTGTGTGGTGACGATCGATCTTGTACAAAATGCAGGTTGTGAGATTGACATCAACTATTTACAAGTTCAATCAATTACTTTAGCTTCAACTTTGTTGATCAATGAGACATGGATGATTTGTCCACCGGGATTTTGTCGTAAGACTCACTATAAACCGTAG
Protein sequence

>Msa1309980

MEVGKSGHSCVVTIDLVQNAGCEIDINYLQVQSITLASTLLINETWMICPPGFCRKTHYKP*