AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0585790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585790 MtrunA17_Chr4g0001401 95.732 164 7 0 1 164 1 164 9.41e-114 319
Msa0585790 MtrunA17_Chr4g0001391 87.805 164 20 0 1 164 1 164 5.27e-106 300
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0585790 AT1G80860.1 75.000 164 41 0 1 164 1 164 4.01e-90 260
Msa0585790 AT1G80860.2 75.000 164 41 0 1 164 1 164 4.01e-90 260

Find 67 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTCTGGCTTTGATTT+TGG 0.091794 4_2:-6890368 None:intergenic
ATCTCACGGGGACGGCTCTT+TGG 0.250844 4_2:+6893761 Msa0585790:three_prime_UTR
ATCAATAGATCCTTCAATTC+TGG 0.252976 4_2:-6890380 None:intergenic
AAGAGACCCTTCAAAGATTA+TGG 0.253526 4_2:+6890541 Msa0585790:CDS
GGAACTGTCCAAATGCAAAA+AGG 0.303744 4_2:-6890655 None:intergenic
ACTGAACTTGATAGCTTGTT+TGG 0.319333 4_2:-6893570 None:intergenic
TAAAATTATTTCAAGCGTTA+AGG 0.334727 4_2:+6893617 Msa0585790:three_prime_UTR
TCTACTATTACCTTTGGAAT+TGG 0.341151 4_2:+6890486 Msa0585790:CDS
TTTGGAACCACGGCCACATT+TGG 0.358197 4_2:-6893552 None:intergenic
TCCCATTCTACTATTACCTT+TGG 0.362758 4_2:+6890480 Msa0585790:CDS
AAAAGGGGCCAAAAGTAAAA+AGG 0.363291 4_2:-6890638 None:intergenic
TTCTTGTTGGACGCGAGTTT+AGG 0.379462 4_2:+6893529 Msa0585790:three_prime_UTR
CAATTGATTCAGCATGTGTT+TGG 0.385430 4_2:+6893432 Msa0585790:exon
AAGAAAGTTACCTGAAGTTA+AGG 0.394770 4_2:-6890676 None:intergenic
CACAAGATGTAGCTTCTTGT+TGG 0.395054 4_2:+6893516 Msa0585790:three_prime_UTR
GATGACGCCGAAAGGGAATT+CGG 0.405825 4_2:-6893260 None:intergenic
TTGGACAGTTCCTTAACTTC+AGG 0.406977 4_2:+6890666 Msa0585790:CDS
TGTAAACTAGCTGGATTGTC+TGG 0.412955 4_2:+6893684 Msa0585790:three_prime_UTR
AAAATCAAAGCCAGAATTGA+AGG 0.415530 4_2:+6890370 Msa0585790:five_prime_UTR
GTCTTGTTGCATGCATTTCA+TGG 0.416231 4_2:+6893314 Msa0585790:CDS
GTGAGATTAACTCGGTTGAC+AGG 0.416348 4_2:-6893745 None:intergenic
TCCAAAGGTAATAGTAGAAT+GGG 0.428209 4_2:-6890481 None:intergenic
TTCCAAAGGTAATAGTAGAA+TGG 0.435473 4_2:-6890482 None:intergenic
TTTGGAATTGGCCACAATCA+TGG 0.439389 4_2:+6890498 Msa0585790:CDS
GGTGAACCCGGCACGTACTA+CGG 0.441291 4_2:+6893205 Msa0585790:CDS
GAGGGGTTGTGTAAACTAGC+TGG 0.467869 4_2:+6893675 Msa0585790:three_prime_UTR
TGGGTTGATTTCTGTGGTAA+AGG 0.470210 4_2:+6890518 Msa0585790:CDS
TTGGAATTGGCCACAATCAT+GGG 0.480101 4_2:+6890499 Msa0585790:CDS
TGGGTAACCGAATTCCCTTT+CGG 0.483152 4_2:+6893253 Msa0585790:CDS
ACATAAGCCATAATCTTTGA+AGG 0.496602 4_2:-6890548 None:intergenic
CACAGGAGGATGTACTGAAA+AGG 0.503339 4_2:-6893340 None:intergenic
TTTGATCCTTTGTGGAAAAG+AGG 0.508643 4_2:-6890344 None:intergenic
GACAGGGTTTATCAGCTGCT+TGG 0.514598 4_2:+6893184 Msa0585790:intron
TCTTGTTGCATGCATTTCAT+GGG 0.516952 4_2:+6893315 Msa0585790:CDS
ACGTACTACGGTGTACGCTT+TGG 0.517896 4_2:+6893217 Msa0585790:CDS
TTGATCCTTTGTGGAAAAGA+GGG 0.529871 4_2:-6890343 None:intergenic
TATCAGCTGCTTGGTGAACC+CGG 0.533558 4_2:+6893193 Msa0585790:CDS
GATCACTGATGACGCCGAAA+GGG 0.542583 4_2:-6893267 None:intergenic
GCTGAATCAATTGAGTGGCT+TGG 0.543638 4_2:-6893422 None:intergenic
GTTTAGGCCAAATGTGGCCG+TGG 0.548256 4_2:+6893545 Msa0585790:three_prime_UTR
AGGTAATAGTAGAATGGGAA+TGG 0.556497 4_2:-6890476 None:intergenic
TGATAGCTTGTTTGGAACCA+CGG 0.558931 4_2:-6893562 None:intergenic
GTCAACCGAGTTAATCTCAC+GGG 0.559924 4_2:+6893748 Msa0585790:three_prime_UTR
CAACAAGAAGCTACATCTTG+TGG 0.560048 4_2:-6893515 None:intergenic
GGATCACTGATGACGCCGAA+AGG 0.568021 4_2:-6893268 None:intergenic
TATGATGCACGTGGAGTCGA+AGG 0.579407 4_2:+6893381 Msa0585790:CDS
TGAGATTAACTCGGTTGACA+GGG 0.581053 4_2:-6893744 None:intergenic
ATCAGTGATCCTCAGTATGT+CGG 0.592522 4_2:+6893280 Msa0585790:CDS
CATAAGCCATAATCTTTGAA+GGG 0.594358 4_2:-6890547 None:intergenic
CAATCATGGGTTGATTTCTG+TGG 0.597484 4_2:+6890512 Msa0585790:CDS
AAAGGGAATTCGGTTACCCA+TGG 0.609636 4_2:-6893250 None:intergenic
ACACATATCCTAAAATCCAC+AGG 0.612366 4_2:-6893357 None:intergenic
ACGCGAGTTTAGGCCAAATG+TGG 0.613292 4_2:+6893539 Msa0585790:three_prime_UTR
ATGATTGTGGCCAATTCCAA+AGG 0.614166 4_2:-6890496 None:intergenic
CAGAAATCAACCCATGATTG+TGG 0.620811 4_2:-6890509 None:intergenic
GCGTACACCGTAGTACGTGC+CGG 0.622625 4_2:-6893212 None:intergenic
CCGTCCCCGTGAGATTAACT+CGG 0.630497 4_2:-6893753 None:intergenic
CGTACACCGTAGTACGTGCC+GGG 0.630869 4_2:-6893211 None:intergenic
ATGATGCTGCCGACATACTG+AGG 0.631811 4_2:-6893289 None:intergenic
CCGAGTTAATCTCACGGGGA+CGG 0.641880 4_2:+6893753 Msa0585790:three_prime_UTR
CACATGCTGAATCAATTGAG+TGG 0.661473 4_2:-6893427 None:intergenic
GAGATTAACTCGGTTGACAG+GGG 0.673866 4_2:-6893743 None:intergenic
TGTCAACCGAGTTAATCTCA+CGG 0.674358 4_2:+6893747 Msa0585790:three_prime_UTR
GCATTGCATAATATATGCAG+AGG 0.683340 4_2:-6893721 None:intergenic
CATATCCTAAAATCCACAGG+AGG 0.712348 4_2:-6893354 None:intergenic
ATATGTGTTTATGATGCACG+TGG 0.741552 4_2:+6893372 Msa0585790:CDS
TCAACCGAGTTAATCTCACG+GGG 0.771126 4_2:+6893749 Msa0585790:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTATATAATGCTATA+TGG + chr4_2:6890787-6890806 Msa0585790:intron 10.0%
!!! AAATTTGTTGTTATAATATT+TGG + chr4_2:6891379-6891398 Msa0585790:intron 10.0%
!!! AATATTAGTATGTTTTAATT+TGG + chr4_2:6892837-6892856 Msa0585790:intron 10.0%
!!! ATATTAGTATGTTTTAATTT+GGG + chr4_2:6892838-6892857 Msa0585790:intron 10.0%
!!! TTTTCATTTTTTGTACTTTT+TGG + chr4_2:6890698-6890717 Msa0585790:intron 15.0%
!!! GATTTTTTTACTCAAAATTA+TGG + chr4_2:6891239-6891258 Msa0585790:intron 15.0%
!! AAAGTAAAATGATATATGTT+TGG - chr4_2:6892055-6892074 None:intergenic 15.0%
!!! ATAAGTTTGTTCTTTTTATT+TGG + chr4_2:6892138-6892157 Msa0585790:intron 15.0%
!!! TTATAACTTTTGTTAATTCA+AGG + chr4_2:6892671-6892690 Msa0585790:intron 15.0%
!!! AAACTATCATTATTTTTTTG+AGG + chr4_2:6893656-6893675 Msa0585790:three_prime_UTR 15.0%
!!! AACTATCATTATTTTTTTGA+GGG + chr4_2:6893657-6893676 Msa0585790:three_prime_UTR 15.0%
!!! AAAAGCAAAGATTTTTTTAG+AGG + chr4_2:6890414-6890433 Msa0585790:five_prime_UTR 20.0%
!! TATGTTAATTCATATGTTCT+CGG + chr4_2:6890953-6890972 Msa0585790:intron 20.0%
!! TTGAGTAAAAAAATCATGTT+TGG - chr4_2:6891234-6891253 None:intergenic 20.0%
!! CTAATATCATAGAAAATGAA+AGG - chr4_2:6891816-6891835 None:intergenic 20.0%
!!! CTTTCATTTTCTATGATATT+AGG + chr4_2:6891814-6891833 Msa0585790:intron 20.0%
!! AAAAATCAACTTAATGTCTT+TGG - chr4_2:6891885-6891904 None:intergenic 20.0%
!!! AAAGACATTAAGTTGATTTT+TGG + chr4_2:6891884-6891903 Msa0585790:intron 20.0%
!! ACAAATTTAATTAGATCCTT+TGG + chr4_2:6892449-6892468 Msa0585790:intron 20.0%
!!! TTTTTGTAATTTCGGTATAT+AGG - chr4_2:6892577-6892596 None:intergenic 20.0%
!!! CAATGATGTTTTTGTAATTT+CGG - chr4_2:6892585-6892604 None:intergenic 20.0%
!! AAAACATTTCATAGCTTATT+TGG - chr4_2:6892916-6892935 None:intergenic 20.0%
!! GATTCTTCATTTATTATTCT+GGG - chr4_2:6892958-6892977 None:intergenic 20.0%
!! TGATTCTTCATTTATTATTC+TGG - chr4_2:6892959-6892978 None:intergenic 20.0%
!! AGCAATACAGTTAAAAATTA+AGG - chr4_2:6893125-6893144 None:intergenic 20.0%
!!! TAATTTTTAACTGTATTGCT+TGG + chr4_2:6893125-6893144 Msa0585790:intron 20.0%
!! TAAAATTATTTCAAGCGTTA+AGG + chr4_2:6893617-6893636 Msa0585790:three_prime_UTR 20.0%
!! AATGATAGTTTACACAAAAA+AGG - chr4_2:6893647-6893666 None:intergenic 20.0%
!!! ACTATCATTATTTTTTTGAG+GGG + chr4_2:6893658-6893677 Msa0585790:three_prime_UTR 20.0%
!!! ATTTTTTTAGAGGAATCTGA+TGG + chr4_2:6890424-6890443 Msa0585790:exon 25.0%
!!! TTTTTTTAGAGGAATCTGAT+GGG + chr4_2:6890425-6890444 Msa0585790:exon 25.0%
!!! TTTTTGTACTTTTTGGTTTC+TGG + chr4_2:6890705-6890724 Msa0585790:intron 25.0%
!!! TTTTGTACTTTTTGGTTTCT+GGG + chr4_2:6890706-6890725 Msa0585790:intron 25.0%
!! ATGTGTTTTATAATCCCTAT+TGG + chr4_2:6890839-6890858 Msa0585790:intron 25.0%
! TATAATCCCTATTGGATATT+AGG + chr4_2:6890847-6890866 Msa0585790:intron 25.0%
! TCTTATCCTAATATCCAATA+GGG - chr4_2:6890856-6890875 None:intergenic 25.0%
! TTCTTATCCTAATATCCAAT+AGG - chr4_2:6890857-6890876 None:intergenic 25.0%
! TTGGATATTAGGATAAGAAA+AGG + chr4_2:6890858-6890877 Msa0585790:intron 25.0%
! TTTCGTTAAAAGTGAGTTTA+AGG - chr4_2:6891310-6891329 None:intergenic 25.0%
! ACAACAAATTTAGTTGTGTT+TGG - chr4_2:6891370-6891389 None:intergenic 25.0%
! AATTCATAACACTGAGATTT+AGG + chr4_2:6891669-6891688 Msa0585790:intron 25.0%
! GATATTAGGATTAGAAAAGA+AGG + chr4_2:6891828-6891847 Msa0585790:intron 25.0%
!! AATTCACAATTTGAGGTAAA+AGG - chr4_2:6891988-6892007 None:intergenic 25.0%
! AGTGTAAAATTCACAATTTG+AGG - chr4_2:6891995-6892014 None:intergenic 25.0%
! TTATGTGTTTAATTCTACAG+TGG - chr4_2:6892122-6892141 None:intergenic 25.0%
! ATGATGAAATGATAGTATTG+AGG + chr4_2:6892161-6892180 Msa0585790:intron 25.0%
! GTGTATAATTCAGATCTTAA+AGG + chr4_2:6892214-6892233 Msa0585790:intron 25.0%
!! AAGAAGATATCCGTATTTTA+TGG + chr4_2:6892356-6892375 Msa0585790:intron 25.0%
! TGAATGTATCTTCTATTAGT+TGG - chr4_2:6892642-6892661 None:intergenic 25.0%
! TTTGTTAATTCAAGGACTAT+AGG + chr4_2:6892679-6892698 Msa0585790:intron 25.0%
! TTGTTAATTCAAGGACTATA+GGG + chr4_2:6892680-6892699 Msa0585790:intron 25.0%
! ACAATGTGATCATTATGATT+AGG - chr4_2:6893159-6893178 None:intergenic 25.0%
! ATTTTGGATTTGATCCTTTG+TGG - chr4_2:6890355-6890374 None:intergenic 30.0%
!!! TTCAATTCTGGCTTTGATTT+TGG - chr4_2:6890371-6890390 None:intergenic 30.0%
AAAATCAAAGCCAGAATTGA+AGG + chr4_2:6890370-6890389 Msa0585790:five_prime_UTR 30.0%
ATCAATAGATCCTTCAATTC+TGG - chr4_2:6890383-6890402 None:intergenic 30.0%
TCCAAAGGTAATAGTAGAAT+GGG - chr4_2:6890484-6890503 None:intergenic 30.0%
TTCCAAAGGTAATAGTAGAA+TGG - chr4_2:6890485-6890504 None:intergenic 30.0%
!! TCTACTATTACCTTTGGAAT+TGG + chr4_2:6890486-6890505 Msa0585790:CDS 30.0%
CATAAGCCATAATCTTTGAA+GGG - chr4_2:6890550-6890569 None:intergenic 30.0%
ACATAAGCCATAATCTTTGA+AGG - chr4_2:6890551-6890570 None:intergenic 30.0%
! TTTCTGTCTCTTCTTTTCAT+TGG + chr4_2:6890609-6890628 Msa0585790:CDS 30.0%
AAGAAAGTTACCTGAAGTTA+AGG - chr4_2:6890679-6890698 None:intergenic 30.0%
TGAATTTGTGGATCTTGTAA+TGG + chr4_2:6890736-6890755 Msa0585790:intron 30.0%
GAATTTGTGGATCTTGTAAT+GGG + chr4_2:6890737-6890756 Msa0585790:intron 30.0%
AATTTGTGGATCTTGTAATG+GGG + chr4_2:6890738-6890757 Msa0585790:intron 30.0%
AGGATAAGAAAAGGAGATTT+AGG + chr4_2:6890867-6890886 Msa0585790:intron 30.0%
GGATAAGAAAAGGAGATTTA+GGG + chr4_2:6890868-6890887 Msa0585790:intron 30.0%
AATTCATATGTTCTCGGTAA+CGG + chr4_2:6890959-6890978 Msa0585790:intron 30.0%
AAAAATAAGCCAATCCAAAC+AGG + chr4_2:6891083-6891102 Msa0585790:intron 30.0%
CGATATATTTGACAGATTCA+TGG - chr4_2:6891528-6891547 None:intergenic 30.0%
GTGTACACATTAGATCTTAA+AGG + chr4_2:6891565-6891584 Msa0585790:intron 30.0%
!!! AGTTGATTTTTGGTATGTTC+AGG + chr4_2:6891894-6891913 Msa0585790:intron 30.0%
!!! GTTGATTTTTGGTATGTTCA+GGG + chr4_2:6891895-6891914 Msa0585790:intron 30.0%
ATGATTGCAGCTTGAAAATT+AGG + chr4_2:6891948-6891967 Msa0585790:intron 30.0%
TACAGTGGTGAAAATTGATT+AGG - chr4_2:6892107-6892126 None:intergenic 30.0%
! GATATCCGTATTTTATGGTT+TGG + chr4_2:6892361-6892380 Msa0585790:intron 30.0%
CAACTCCAAACCATAAAATA+CGG - chr4_2:6892369-6892388 None:intergenic 30.0%
!! TTTTGAGTGAACACTCATTT+TGG + chr4_2:6892518-6892537 Msa0585790:intron 30.0%
! TATCTTCTATTAGTTGGCTT+GGG - chr4_2:6892636-6892655 None:intergenic 30.0%
!! ATTTTGGTCCTTAAATGTGT+CGG - chr4_2:6892713-6892732 None:intergenic 30.0%
!!! GAAAGGAGTAAGTGTTATTT+TGG - chr4_2:6892729-6892748 None:intergenic 30.0%
! TGTTTGGTAGTAATGTAGAA+AGG - chr4_2:6892751-6892770 None:intergenic 30.0%
ACATCACAGTTTCATCAAAA+CGG - chr4_2:6892997-6893016 None:intergenic 30.0%
!! CACTTTTGAAGATACAATGA+CGG - chr4_2:6893073-6893092 None:intergenic 30.0%
TAAGGCTCAATGAGAATATT+CGG - chr4_2:6893107-6893126 None:intergenic 30.0%
TTGATCCTTTGTGGAAAAGA+GGG - chr4_2:6890346-6890365 None:intergenic 35.0%
TTTGATCCTTTGTGGAAAAG+AGG - chr4_2:6890347-6890366 None:intergenic 35.0%
!! ATGGGTATTTTTGCTGCTAT+TGG + chr4_2:6890443-6890462 Msa0585790:CDS 35.0%
AGGTAATAGTAGAATGGGAA+TGG - chr4_2:6890479-6890498 None:intergenic 35.0%
TCCCATTCTACTATTACCTT+TGG + chr4_2:6890480-6890499 Msa0585790:CDS 35.0%
AAGAGACCCTTCAAAGATTA+TGG + chr4_2:6890541-6890560 Msa0585790:CDS 35.0%
AAAAGGGGCCAAAAGTAAAA+AGG - chr4_2:6890641-6890660 None:intergenic 35.0%
AACTGTCCAAATGCAAAAAG+GGG - chr4_2:6890656-6890675 None:intergenic 35.0%
GAACTGTCCAAATGCAAAAA+GGG - chr4_2:6890657-6890676 None:intergenic 35.0%
!!! CTGGGTTTTGTTTGAATTTG+TGG + chr4_2:6890724-6890743 Msa0585790:intron 35.0%
! AATGCTATATGGTGCTGAAT+TGG + chr4_2:6890798-6890817 Msa0585790:intron 35.0%
! TCAATGCTTGGATTCACTTT+TGG + chr4_2:6891112-6891131 Msa0585790:intron 35.0%
CAACTCAGAATCAATTGTAG+CGG - chr4_2:6891186-6891205 None:intergenic 35.0%
! CTACAATTGATTCTGAGTTG+AGG + chr4_2:6891186-6891205 Msa0585790:intron 35.0%
! AGTTGTGTTTGGTTCAAATG+TGG - chr4_2:6891359-6891378 None:intergenic 35.0%
ATGGTGTATCACAATGATTG+TGG - chr4_2:6891509-6891528 None:intergenic 35.0%
TCTCAATTCGATATGCTAAC+TGG + chr4_2:6891634-6891653 Msa0585790:intron 35.0%
AGTAGTGAGTAGCGAATAAT+GGG + chr4_2:6891773-6891792 Msa0585790:intron 35.0%
AGGCGAAATCAATTCGAATT+AGG - chr4_2:6891923-6891942 None:intergenic 35.0%
TCAAGCTGCAATCATTCTAA+AGG - chr4_2:6891943-6891962 None:intergenic 35.0%
ATTATACACCACAGCAAAGT+GGG - chr4_2:6892203-6892222 None:intergenic 35.0%
AATTATACACCACAGCAAAG+TGG - chr4_2:6892204-6892223 None:intergenic 35.0%
TCTCAATTTGATATGCCGAT+TGG + chr4_2:6892283-6892302 Msa0585790:intron 35.0%
ATTTACACCATTGCACCAAT+CGG - chr4_2:6892301-6892320 None:intergenic 35.0%
! AGTTGATATGTCTGTGTTCT+TGG + chr4_2:6892384-6892403 Msa0585790:intron 35.0%
GAGAATATCACAATTGCAGA+AGG + chr4_2:6892476-6892495 Msa0585790:intron 35.0%
TGGTTCTTATGTGTATGAAG+TGG + chr4_2:6892538-6892557 Msa0585790:intron 35.0%
GTATCTTCTATTAGTTGGCT+TGG - chr4_2:6892637-6892656 None:intergenic 35.0%
! TTTTGGTCCTTAAATGTGTC+GGG - chr4_2:6892712-6892731 None:intergenic 35.0%
GGTAGTAATGTAGAAAGGAA+AGG - chr4_2:6892746-6892765 None:intergenic 35.0%
GCTTTCTTGGTAAGATTGTT+TGG - chr4_2:6892767-6892786 None:intergenic 35.0%
!! TTTTGAAGATACAATGACGG+AGG - chr4_2:6893070-6893089 None:intergenic 35.0%
AAGGCTCAATGAGAATATTC+GGG - chr4_2:6893106-6893125 None:intergenic 35.0%
TGATCACATTGTCAAATGAC+AGG + chr4_2:6893167-6893186 Msa0585790:intron 35.0%
GATCACATTGTCAAATGACA+GGG + chr4_2:6893168-6893187 Msa0585790:intron 35.0%
! CTTTGGAAAAACTCTTCCAT+GGG + chr4_2:6893234-6893253 Msa0585790:CDS 35.0%
TCTTGTTGCATGCATTTCAT+GGG + chr4_2:6893315-6893334 Msa0585790:CDS 35.0%
ACACATATCCTAAAATCCAC+AGG - chr4_2:6893360-6893379 None:intergenic 35.0%
ATATGTGTTTATGATGCACG+TGG + chr4_2:6893372-6893391 Msa0585790:CDS 35.0%
! CAATTGATTCAGCATGTGTT+TGG + chr4_2:6893432-6893451 Msa0585790:exon 35.0%
!! ACTGAACTTGATAGCTTGTT+TGG - chr4_2:6893573-6893592 None:intergenic 35.0%
GCATTGCATAATATATGCAG+AGG - chr4_2:6893724-6893743 None:intergenic 35.0%
! TGTAACCCTCTTTTCCACAA+AGG + chr4_2:6890338-6890357 Msa0585790:five_prime_UTR 40.0%
ATGATTGTGGCCAATTCCAA+AGG - chr4_2:6890499-6890518 None:intergenic 40.0%
! TTTGGAATTGGCCACAATCA+TGG + chr4_2:6890498-6890517 Msa0585790:CDS 40.0%
TTGGAATTGGCCACAATCAT+GGG + chr4_2:6890499-6890518 Msa0585790:CDS 40.0%
CAGAAATCAACCCATGATTG+TGG - chr4_2:6890512-6890531 None:intergenic 40.0%
!! CAATCATGGGTTGATTTCTG+TGG + chr4_2:6890512-6890531 Msa0585790:CDS 40.0%
!! TGGGTTGATTTCTGTGGTAA+AGG + chr4_2:6890518-6890537 Msa0585790:CDS 40.0%
! TTTTGGCCCCTTTTTGCATT+TGG + chr4_2:6890647-6890666 Msa0585790:CDS 40.0%
GGAACTGTCCAAATGCAAAA+AGG - chr4_2:6890658-6890677 None:intergenic 40.0%
TTGGACAGTTCCTTAACTTC+AGG + chr4_2:6890666-6890685 Msa0585790:CDS 40.0%
! AAGGAGATTTAGGGTCTGTT+TGG + chr4_2:6890877-6890896 Msa0585790:intron 40.0%
TGACTTAAGCCTGTTTGGAT+TGG - chr4_2:6891095-6891114 None:intergenic 40.0%
!! AGCATTGACTTAAGCCTGTT+TGG - chr4_2:6891100-6891119 None:intergenic 40.0%
AACAGGCTTAAGTCAATGCT+TGG + chr4_2:6891100-6891119 Msa0585790:intron 40.0%
CAATTCGATATGCTAACTGG+TGG + chr4_2:6891637-6891656 Msa0585790:intron 40.0%
GATATCCTGATGCCATGATA+CGG + chr4_2:6891712-6891731 Msa0585790:intron 40.0%
TGCATAGAAACTCCGTATCA+TGG - chr4_2:6891727-6891746 None:intergenic 40.0%
GAGTAGTGAGTAGCGAATAA+TGG + chr4_2:6891772-6891791 Msa0585790:intron 40.0%
GTAGTGAGTAGCGAATAATG+GGG + chr4_2:6891774-6891793 Msa0585790:intron 40.0%
TTATACACCACAGCAAAGTG+GGG - chr4_2:6892202-6892221 None:intergenic 40.0%
!! ATCTTACCAAGAAAGCACTC+TGG + chr4_2:6892771-6892790 Msa0585790:intron 40.0%
!! TGTTTTCCAGAGTGCTTTCT+TGG - chr4_2:6892780-6892799 None:intergenic 40.0%
! TGATTTGCAGAATGAACTGC+AGG - chr4_2:6892888-6892907 None:intergenic 40.0%
CAAAACGGTGGTATTCATCT+TGG - chr4_2:6892982-6893001 None:intergenic 40.0%
TCACAGTTTCATCAAAACGG+TGG - chr4_2:6892994-6893013 None:intergenic 40.0%
!! GCTTTGGAAAAACTCTTCCA+TGG + chr4_2:6893233-6893252 Msa0585790:CDS 40.0%
ATCAGTGATCCTCAGTATGT+CGG + chr4_2:6893280-6893299 Msa0585790:CDS 40.0%
! GTCTTGTTGCATGCATTTCA+TGG + chr4_2:6893314-6893333 Msa0585790:CDS 40.0%
! TACATCCTCCTGTGGATTTT+AGG + chr4_2:6893349-6893368 Msa0585790:CDS 40.0%
CATATCCTAAAATCCACAGG+AGG - chr4_2:6893357-6893376 None:intergenic 40.0%
CACATGCTGAATCAATTGAG+TGG - chr4_2:6893430-6893449 None:intergenic 40.0%
CAACAAGAAGCTACATCTTG+TGG - chr4_2:6893518-6893537 None:intergenic 40.0%
CACAAGATGTAGCTTCTTGT+TGG + chr4_2:6893516-6893535 Msa0585790:three_prime_UTR 40.0%
!! TGATAGCTTGTTTGGAACCA+CGG - chr4_2:6893565-6893584 None:intergenic 40.0%
TGTAAACTAGCTGGATTGTC+TGG + chr4_2:6893684-6893703 Msa0585790:three_prime_UTR 40.0%
TGAGATTAACTCGGTTGACA+GGG - chr4_2:6893747-6893766 None:intergenic 40.0%
TGTCAACCGAGTTAATCTCA+CGG + chr4_2:6893747-6893766 Msa0585790:three_prime_UTR 40.0%
! GGCCTCCTCCTTTTTACTTT+TGG + chr4_2:6890630-6890649 Msa0585790:CDS 45.0%
GGCCAAAAGTAAAAAGGAGG+AGG - chr4_2:6890635-6890654 None:intergenic 45.0%
AGGGGCCAAAAGTAAAAAGG+AGG - chr4_2:6890638-6890657 None:intergenic 45.0%
! GCTATATGGTGCTGAATTGG+AGG + chr4_2:6890801-6890820 Msa0585790:intron 45.0%
TCGATACATGCACGTGAAAG+TGG - chr4_2:6891271-6891290 None:intergenic 45.0%
AAACTCCGTATCATGGCATC+AGG - chr4_2:6891720-6891739 None:intergenic 45.0%
ATACTTTCCCCACTTTGCTG+TGG + chr4_2:6892192-6892211 Msa0585790:intron 45.0%
! TGATATGCCGATTGGTGCAA+TGG + chr4_2:6892291-6892310 Msa0585790:intron 45.0%
TGTGATATTCTCGCAGCCAA+AGG - chr4_2:6892468-6892487 None:intergenic 45.0%
GTTAGTGCCCGACACATTTA+AGG + chr4_2:6892702-6892721 Msa0585790:intron 45.0%
AAAGGGAATTCGGTTACCCA+TGG - chr4_2:6893253-6893272 None:intergenic 45.0%
TGGGTAACCGAATTCCCTTT+CGG + chr4_2:6893253-6893272 Msa0585790:CDS 45.0%
CACAGGAGGATGTACTGAAA+AGG - chr4_2:6893343-6893362 None:intergenic 45.0%
! CTTTTCAGTACATCCTCCTG+TGG + chr4_2:6893341-6893360 Msa0585790:CDS 45.0%
GCTGAATCAATTGAGTGGCT+TGG - chr4_2:6893425-6893444 None:intergenic 45.0%
CTCGAGACGCAAAAATCAAG+AGG - chr4_2:6893469-6893488 None:intergenic 45.0%
! CTCTTGATTTTTGCGTCTCG+AGG + chr4_2:6893467-6893486 Msa0585790:three_prime_UTR 45.0%
TTCTTGTTGGACGCGAGTTT+AGG + chr4_2:6893529-6893548 Msa0585790:three_prime_UTR 45.0%
GAGATTAACTCGGTTGACAG+GGG - chr4_2:6893746-6893765 None:intergenic 45.0%
GTGAGATTAACTCGGTTGAC+AGG - chr4_2:6893748-6893767 None:intergenic 45.0%
GTCAACCGAGTTAATCTCAC+GGG + chr4_2:6893748-6893767 Msa0585790:three_prime_UTR 45.0%
TCAACCGAGTTAATCTCACG+GGG + chr4_2:6893749-6893768 Msa0585790:three_prime_UTR 45.0%
!! TTATATAAAAATTACATAAA+AGG - chr4_2:6890780-6890799 None:intergenic 5.0%
GACAGGGTTTATCAGCTGCT+TGG + chr4_2:6893184-6893203 Msa0585790:intron 50.0%
! TATCAGCTGCTTGGTGAACC+CGG + chr4_2:6893193-6893212 Msa0585790:CDS 50.0%
ACGTACTACGGTGTACGCTT+TGG + chr4_2:6893217-6893236 Msa0585790:CDS 50.0%
GATGACGCCGAAAGGGAATT+CGG - chr4_2:6893263-6893282 None:intergenic 50.0%
! GATCACTGATGACGCCGAAA+GGG - chr4_2:6893270-6893289 None:intergenic 50.0%
ATGATGCTGCCGACATACTG+AGG - chr4_2:6893292-6893311 None:intergenic 50.0%
TATGATGCACGTGGAGTCGA+AGG + chr4_2:6893381-6893400 Msa0585790:CDS 50.0%
ACGCGAGTTTAGGCCAAATG+TGG + chr4_2:6893539-6893558 Msa0585790:three_prime_UTR 50.0%
! TTTGGAACCACGGCCACATT+TGG - chr4_2:6893555-6893574 None:intergenic 50.0%
GAGGGGTTGTGTAAACTAGC+TGG + chr4_2:6893675-6893694 Msa0585790:three_prime_UTR 50.0%
TGCAGGTAGCAGTCTCTTGC+TGG - chr4_2:6892871-6892890 None:intergenic 55.0%
! GGATCACTGATGACGCCGAA+AGG - chr4_2:6893271-6893290 None:intergenic 55.0%
GTTTAGGCCAAATGTGGCCG+TGG + chr4_2:6893545-6893564 Msa0585790:three_prime_UTR 55.0%
CCGTCCCCGTGAGATTAACT+CGG - chr4_2:6893756-6893775 None:intergenic 55.0%
CCGAGTTAATCTCACGGGGA+CGG + chr4_2:6893753-6893772 Msa0585790:three_prime_UTR 55.0%
GGTGAACCCGGCACGTACTA+CGG + chr4_2:6893205-6893224 Msa0585790:CDS 60.0%
CGTACACCGTAGTACGTGCC+GGG - chr4_2:6893214-6893233 None:intergenic 60.0%
GCGTACACCGTAGTACGTGC+CGG - chr4_2:6893215-6893234 None:intergenic 60.0%
ATCTCACGGGGACGGCTCTT+TGG + chr4_2:6893761-6893780 Msa0585790:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 6890238 6893854 6890238 ID=Msa0585790;Name=Msa0585790
chr4_2 mRNA 6890238 6893854 6890238 ID=Msa0585790-mRNA-1;Parent=Msa0585790;Name=Msa0585790-mRNA-1;_AED=0.16;_eAED=0.16;_QI=205|1|1|1|1|1|2|416|164
chr4_2 exon 6890238 6890687 6890238 ID=Msa0585790-mRNA-1:exon:1728;Parent=Msa0585790-mRNA-1
chr4_2 exon 6893189 6893854 6893189 ID=Msa0585790-mRNA-1:exon:1729;Parent=Msa0585790-mRNA-1
chr4_2 five_prime_UTR 6890238 6890442 6890238 ID=Msa0585790-mRNA-1:five_prime_utr;Parent=Msa0585790-mRNA-1
chr4_2 CDS 6890443 6890687 6890443 ID=Msa0585790-mRNA-1:cds;Parent=Msa0585790-mRNA-1
chr4_2 CDS 6893189 6893438 6893189 ID=Msa0585790-mRNA-1:cds;Parent=Msa0585790-mRNA-1
chr4_2 three_prime_UTR 6893439 6893854 6893439 ID=Msa0585790-mRNA-1:three_prime_utr;Parent=Msa0585790-mRNA-1
Gene Sequence

>Msa0585790

ATGGGTATTTTTGCTGCTATTGGAGTGTTATCACCATTCCCATTCTACTATTACCTTTGGAATTGGCCACAATCATGGGTTGATTTCTGTGGTAAAGGAAGAGACCCTTCAAAGATTATGGCTTATGTTGCTCATTTCTTGAAGATAATTCAGTTCATTTCACTCTTTTCTGTCTCTTCTTTTCATTGGCCTCCTCCTTTTTACTTTTGGCCCCTTTTTGCATTTGGACAGTTCCTTAACTTCAGGGTTTATCAGCTGCTTGGTGAACCCGGCACGTACTACGGTGTACGCTTTGGAAAAACTCTTCCATGGGTAACCGAATTCCCTTTCGGCGTCATCAGTGATCCTCAGTATGTCGGCAGCATCATGAGTCTTGTTGCATGCATTTCATGGGTTCCTTTTCAGTACATCCTCCTGTGGATTTTAGGATATGTGTTTATGATGCACGTGGAGTCGAAGGAAGATCAATCAACTCGAGCCAAGCCACTCAATTGA
Protein sequence

>Msa0585790

MGIFAAIGVLSPFPFYYYLWNWPQSWVDFCGKGRDPSKIMAYVAHFLKIIQFISLFSVSSFHWPPPFYFWPLFAFGQFLNFRVYQLLGEPGTYYGVRFGKTLPWVTEFPFGVISDPQYVGSIMSLVACISWVPFQYILLWILGYVFMMHVESKEDQSTRAKPLN*