AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0625840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625840 MtrunA17_Chr4g0001401 95.732 164 7 0 1 164 1 164 9.41e-114 319
Msa0625840 MtrunA17_Chr4g0001391 87.805 164 20 0 1 164 1 164 5.27e-106 300
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0625840 AT1G80860.1 75.000 164 41 0 1 164 1 164 4.01e-90 260
Msa0625840 AT1G80860.2 75.000 164 41 0 1 164 1 164 4.01e-90 260

Find 59 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAATTCTGGCTTTGATTT+TGG 0.091794 4_3:+5177163 None:intergenic
ATCAATAGATCCTTCAATTC+TGG 0.252976 4_3:+5177151 None:intergenic
AAGAGACCCTTCAAAGATTA+TGG 0.253526 4_3:-5176990 Msa0625840:CDS
GGAACTGTCCAAATGCAAAA+AGG 0.303744 4_3:+5176876 None:intergenic
ACTGAACTTGATAGCTTGTT+TGG 0.319333 4_3:+5173966 None:intergenic
TAAAATTATTTCAAGCGTTA+AGG 0.334727 4_3:-5173919 Msa0625840:three_prime_UTR
TCTACTATTACCTTTGGAAT+TGG 0.341151 4_3:-5177045 Msa0625840:CDS
TTCCATTCTACTATTACCTT+TGG 0.347080 4_3:-5177051 Msa0625840:CDS
TTTGGAACCACGGCCACATT+TGG 0.358197 4_3:+5173984 None:intergenic
AAAAGGGGCCAAAAGTAAAA+AGG 0.363291 4_3:+5176893 None:intergenic
TTCTTGTTGGACGCGAGTTT+AGG 0.379462 4_3:-5174007 Msa0625840:three_prime_UTR
CAATTGATTCAGCATGTGTT+TGG 0.385430 4_3:-5174104 Msa0625840:exon
AAGAAAGTTACCTGAAGTTA+AGG 0.394770 4_3:+5176855 None:intergenic
CACAAGATGTAGCTTCTTGT+TGG 0.395054 4_3:-5174020 Msa0625840:three_prime_UTR
GATGACGCCGAAAGGGAATT+CGG 0.405825 4_3:+5174276 None:intergenic
TTGGACAGTTCCTTAACTTC+AGG 0.406977 4_3:-5176865 Msa0625840:intron
AAAATCAAAGCCAGAATTGA+AGG 0.415530 4_3:-5177161 Msa0625840:five_prime_UTR
GTCTTGTTGCATGCATTTCA+TGG 0.416231 4_3:-5174222 Msa0625840:CDS
TTTGGAATTGGCCACAATCA+TGG 0.439389 4_3:-5177033 Msa0625840:CDS
GGTGAACCCGGCACGTACTA+CGG 0.441291 4_3:-5174331 Msa0625840:CDS
AGGTAATAGTAGAATGGAAA+TGG 0.454712 4_3:+5177055 None:intergenic
TGGGTTGATTTCTGTGGTAA+AGG 0.470210 4_3:-5177013 Msa0625840:CDS
TTGGAATTGGCCACAATCAT+GGG 0.480101 4_3:-5177032 Msa0625840:CDS
TGGGTAACCGAATTCCCTTT+CGG 0.483152 4_3:-5174283 Msa0625840:CDS
TTCCAAAGGTAATAGTAGAA+TGG 0.492104 4_3:+5177049 None:intergenic
ACATAAGCCATAATCTTTGA+AGG 0.496602 4_3:+5176983 None:intergenic
TTTGATCCTTTGTCGAAAAG+AGG 0.496861 4_3:+5177187 None:intergenic
CACAGGAGGATGTACTGAAA+AGG 0.503339 4_3:+5174196 None:intergenic
GACAGGGTTTATCAGCTGCT+TGG 0.514598 4_3:-5174352 Msa0625840:intron
TCTTGTTGCATGCATTTCAT+GGG 0.516952 4_3:-5174221 Msa0625840:CDS
ACGTACTACGGTGTACGCTT+TGG 0.517896 4_3:-5174319 Msa0625840:CDS
TATCAGCTGCTTGGTGAACC+CGG 0.533558 4_3:-5174343 Msa0625840:CDS
TTCAATGGAGCTTGATGATG+TGG 0.542527 4_3:+5173756 None:intergenic
GATCACTGATGACGCCGAAA+GGG 0.542583 4_3:+5174269 None:intergenic
GCTGAATCAATTGAGTGGCT+TGG 0.543638 4_3:+5174114 None:intergenic
GTTTAGGCCAAATGTGGCCG+TGG 0.548256 4_3:-5173991 Msa0625840:three_prime_UTR
TTGATCCTTTGTCGAAAAGA+GGG 0.550624 4_3:+5177188 None:intergenic
AAAATTAAGAAATCAAATGA+GGG 0.556523 4_3:-5173828 Msa0625840:three_prime_UTR
TGATAGCTTGTTTGGAACCA+CGG 0.558931 4_3:+5173974 None:intergenic
CAACAAGAAGCTACATCTTG+TGG 0.560048 4_3:+5174021 None:intergenic
GGATCACTGATGACGCCGAA+AGG 0.568021 4_3:+5174268 None:intergenic
TATGATGCACGTGGAGTCGA+AGG 0.579407 4_3:-5174155 Msa0625840:CDS
TACATTTAGAGCGTCTCCAA+TGG 0.587734 4_3:+5173686 None:intergenic
AACATAATAGGACTCTCCAT+TGG 0.590478 4_3:-5173702 Msa0625840:three_prime_UTR
ATCAGTGATCCTCAGTATGT+CGG 0.592522 4_3:-5174256 Msa0625840:CDS
CATAAGCCATAATCTTTGAA+GGG 0.594358 4_3:+5176984 None:intergenic
CAATCATGGGTTGATTTCTG+TGG 0.597484 4_3:-5177019 Msa0625840:CDS
AAAGGGAATTCGGTTACCCA+TGG 0.609636 4_3:+5174286 None:intergenic
ACACATATCCTAAAATCCAC+AGG 0.612366 4_3:+5174179 None:intergenic
ACGCGAGTTTAGGCCAAATG+TGG 0.613292 4_3:-5173997 Msa0625840:three_prime_UTR
ATGATTGTGGCCAATTCCAA+AGG 0.614166 4_3:+5177035 None:intergenic
CAGAAATCAACCCATGATTG+TGG 0.620811 4_3:+5177022 None:intergenic
GCGTACACCGTAGTACGTGC+CGG 0.622625 4_3:+5174324 None:intergenic
CGTACACCGTAGTACGTGCC+GGG 0.630869 4_3:+5174325 None:intergenic
ATGATGCTGCCGACATACTG+AGG 0.631811 4_3:+5174247 None:intergenic
CACATGCTGAATCAATTGAG+TGG 0.661473 4_3:+5174109 None:intergenic
AAATTAAGAAATCAAATGAG+GGG 0.683127 4_3:-5173827 Msa0625840:three_prime_UTR
CATATCCTAAAATCCACAGG+AGG 0.712348 4_3:+5174182 None:intergenic
ATATGTGTTTATGATGCACG+TGG 0.741552 4_3:-5174164 Msa0625840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATAAAAATTAAATAAA+AGG + chr4_3:5174199-5174218 None:intergenic 0.0%
!!! AATTTTTATATAATGCTATA+TGG - chr4_3:5174206-5174225 Msa0625840:CDS 10.0%
!!! AATATTAGTATGTTTTAATT+TGG - chr4_3:5176251-5176270 Msa0625840:intron 10.0%
!!! ATATTAGTATGTTTTAATTT+GGG - chr4_3:5176252-5176271 Msa0625840:intron 10.0%
!!! TTTTCATTTTTTGTACTTTT+TGG - chr4_3:5174117-5174136 Msa0625840:exon 15.0%
!!! GATTTTTTTACTCAAAATTA+TGG - chr4_3:5174681-5174700 Msa0625840:intron 15.0%
!!! AAATTTGTTGTTATAGTATT+TGG - chr4_3:5174821-5174840 Msa0625840:intron 15.0%
!! AAAGTAAAATGATATATGTT+TGG + chr4_3:5175489-5175508 None:intergenic 15.0%
!!! ATAAGTTTGTTCTTTTTATT+TGG - chr4_3:5175572-5175591 Msa0625840:intron 15.0%
!!! TTATAACTTTTGTTAATTCA+AGG - chr4_3:5176085-5176104 Msa0625840:intron 15.0%
!! AAAACATTTCATAGTTTATT+TGG + chr4_3:5176330-5176349 None:intergenic 15.0%
!!! AAACTATCATTATTTTTTTG+AGG - chr4_3:5177070-5177089 Msa0625840:CDS 15.0%
!!! AACTATCATTATTTTTTTGA+GGG - chr4_3:5177071-5177090 Msa0625840:CDS 15.0%
!! AAAAATTAAGAAATCAAATG+AGG - chr4_3:5177121-5177140 Msa0625840:five_prime_UTR 15.0%
!! AAAATTAAGAAATCAAATGA+GGG - chr4_3:5177122-5177141 Msa0625840:five_prime_UTR 15.0%
!!! AAAAGCAAAGATTTTTTTAG+AGG - chr4_3:5173833-5173852 Msa0625840:three_prime_UTR 20.0%
!!! TTTAGTTTTATAATCCCTAT+TGG - chr4_3:5174281-5174300 Msa0625840:CDS 20.0%
!! TTGAGTAAAAAAATCATGTT+TGG + chr4_3:5174676-5174695 None:intergenic 20.0%
!! CTAATATCATAGAAAATGAA+AGG + chr4_3:5175251-5175270 None:intergenic 20.0%
!!! CTTTCATTTTCTATGATATT+AGG - chr4_3:5175249-5175268 Msa0625840:intron 20.0%
!! AAAAATCAACTTAATGTCTT+TGG + chr4_3:5175320-5175339 None:intergenic 20.0%
!!! AAAGACATTAAGTTGATTTT+TGG - chr4_3:5175319-5175338 Msa0625840:intron 20.0%
!!! TTTTTGTAATTTCGGTATAT+AGG + chr4_3:5175991-5176010 None:intergenic 20.0%
!!! CAATGATGTTTTTGTAATTT+CGG + chr4_3:5175999-5176018 None:intergenic 20.0%
!! GATTCTTCATTTATTATTCT+GGG + chr4_3:5176372-5176391 None:intergenic 20.0%
!! TGATTCTTCATTTATTATTC+TGG + chr4_3:5176373-5176392 None:intergenic 20.0%
!! AGCAATACAGTTAAAAATTA+AGG + chr4_3:5176539-5176558 None:intergenic 20.0%
!!! TAATTTTTAACTGTATTGCT+TGG - chr4_3:5176539-5176558 Msa0625840:intron 20.0%
!! TAAAATTATTTCAAGCGTTA+AGG - chr4_3:5177031-5177050 Msa0625840:CDS 20.0%
!!! ACTATCATTATTTTTTTGAG+GGG - chr4_3:5177072-5177091 Msa0625840:CDS 20.0%
!! AAATTAAGAAATCAAATGAG+GGG - chr4_3:5177123-5177142 Msa0625840:five_prime_UTR 20.0%
!!! CTAATTTTAAGGAACATAAT+AGG - chr4_3:5177236-5177255 Msa0625840:five_prime_UTR 20.0%
!!! ATTTTTTTAGAGGAATCTGA+TGG - chr4_3:5173843-5173862 Msa0625840:three_prime_UTR 25.0%
!!! TTTTTTTAGAGGAATCTGAT+GGG - chr4_3:5173844-5173863 Msa0625840:three_prime_UTR 25.0%
!!! TTTTTGTACTTTTTGGTTTC+TGG - chr4_3:5174124-5174143 Msa0625840:CDS 25.0%
!!! TTTTGTACTTTTTGGTTTCT+GGG - chr4_3:5174125-5174144 Msa0625840:CDS 25.0%
! TATAATCCCTATTGGATATT+AGG - chr4_3:5174289-5174308 Msa0625840:CDS 25.0%
! TCTTATCCTAATATCCAATA+GGG + chr4_3:5174298-5174317 None:intergenic 25.0%
! TTCTTATCCTAATATCCAAT+AGG + chr4_3:5174299-5174318 None:intergenic 25.0%
! TTGGATATTAGGATAAGAAA+AGG - chr4_3:5174300-5174319 Msa0625840:CDS 25.0%
! TTTCGTTAAAAGTGAGTTTA+AGG + chr4_3:5174752-5174771 None:intergenic 25.0%
! ACAACAAATTTAGTTGTGTT+TGG + chr4_3:5174812-5174831 None:intergenic 25.0%
! AATTCATAACACTGAGATTT+AGG - chr4_3:5175111-5175130 Msa0625840:intron 25.0%
! GATATTAGGATTAGAAAAGA+AGG - chr4_3:5175263-5175282 Msa0625840:intron 25.0%
!! AATTCACAATTTGAGGTAAA+AGG + chr4_3:5175422-5175441 None:intergenic 25.0%
! AGTGTAAAATTCACAATTTG+AGG + chr4_3:5175429-5175448 None:intergenic 25.0%
!!! TTATGTGTTTTATTCTACAG+TGG + chr4_3:5175556-5175575 None:intergenic 25.0%
! ATGATGAAATGATAGTATTG+AGG - chr4_3:5175595-5175614 Msa0625840:intron 25.0%
! GTGTATAATTCAGATCTTAA+AGG - chr4_3:5175648-5175667 Msa0625840:intron 25.0%
!! AAGAAGATATCCGTATTTTA+TGG - chr4_3:5175790-5175809 Msa0625840:intron 25.0%
!! AGAAGATATCCGTATTTTAT+GGG - chr4_3:5175791-5175810 Msa0625840:intron 25.0%
! ACGAATTTAATTAGATCCTT+TGG - chr4_3:5175863-5175882 Msa0625840:intron 25.0%
! TTTGTTAATTCAAGGACTAT+AGG - chr4_3:5176093-5176112 Msa0625840:intron 25.0%
! TTGTTAATTCAAGGACTATA+GGG - chr4_3:5176094-5176113 Msa0625840:intron 25.0%
!! CATAAAAGTTATGTTTTCCA+GGG + chr4_3:5176205-5176224 None:intergenic 25.0%
!! ACATAAAAGTTATGTTTTCC+AGG + chr4_3:5176206-5176225 None:intergenic 25.0%
! ACAATGTGATCATTATGATT+AGG + chr4_3:5176573-5176592 None:intergenic 25.0%
! AATGATAGTTTACGCAAAAA+AGG + chr4_3:5177061-5177080 None:intergenic 25.0%
! CTCCATTGAAAAACAATTAA+GGG - chr4_3:5177207-5177226 Msa0625840:five_prime_UTR 25.0%
!!! ATCCCTTAATTGTTTTTCAA+TGG + chr4_3:5177212-5177231 None:intergenic 25.0%
!!! TTCAATTCTGGCTTTGATTT+TGG + chr4_3:5173790-5173809 None:intergenic 30.0%
AAAATCAAAGCCAGAATTGA+AGG - chr4_3:5173789-5173808 Msa0625840:three_prime_UTR 30.0%
ATCAATAGATCCTTCAATTC+TGG + chr4_3:5173802-5173821 None:intergenic 30.0%
AGGTAATAGTAGAATGGAAA+TGG + chr4_3:5173898-5173917 None:intergenic 30.0%
TTCCATTCTACTATTACCTT+TGG - chr4_3:5173899-5173918 Msa0625840:three_prime_UTR 30.0%
TTCCAAAGGTAATAGTAGAA+TGG + chr4_3:5173904-5173923 None:intergenic 30.0%
!! TCTACTATTACCTTTGGAAT+TGG - chr4_3:5173905-5173924 Msa0625840:three_prime_UTR 30.0%
CATAAGCCATAATCTTTGAA+GGG + chr4_3:5173969-5173988 None:intergenic 30.0%
ACATAAGCCATAATCTTTGA+AGG + chr4_3:5173970-5173989 None:intergenic 30.0%
! TTTCTGTCTCTTCTTTTCAT+TGG - chr4_3:5174028-5174047 Msa0625840:three_prime_UTR 30.0%
AAGAAAGTTACCTGAAGTTA+AGG + chr4_3:5174098-5174117 None:intergenic 30.0%
TGAATTTGTGGATCTTGTAA+TGG - chr4_3:5174155-5174174 Msa0625840:CDS 30.0%
GAATTTGTGGATCTTGTAAT+GGG - chr4_3:5174156-5174175 Msa0625840:CDS 30.0%
AATTTGTGGATCTTGTAATG+GGG - chr4_3:5174157-5174176 Msa0625840:CDS 30.0%
AGGATAAGAAAAGGAGATTT+AGG - chr4_3:5174309-5174328 Msa0625840:CDS 30.0%
GGATAAGAAAAGGAGATTTA+GGG - chr4_3:5174310-5174329 Msa0625840:CDS 30.0%
AATTCATATGTTCTCGCTAA+CGG - chr4_3:5174401-5174420 Msa0625840:intron 30.0%
AAAAATAAGCCAATCCAAAC+AGG - chr4_3:5174525-5174544 Msa0625840:intron 30.0%
CAACTCATAATCAATTGTAG+CGG + chr4_3:5174628-5174647 None:intergenic 30.0%
CTACAATTGATTATGAGTTG+AGG - chr4_3:5174628-5174647 Msa0625840:intron 30.0%
! TAGTTGTGTTTGGTTCAAAT+GGG + chr4_3:5174802-5174821 None:intergenic 30.0%
! TTAGTTGTGTTTGGTTCAAA+TGG + chr4_3:5174803-5174822 None:intergenic 30.0%
CGATATATTTGACAGATTCA+TGG + chr4_3:5174970-5174989 None:intergenic 30.0%
GTGTACACATTAGATCTTAA+AGG - chr4_3:5175007-5175026 Msa0625840:intron 30.0%
TACAGTGGTGAAAATTGATT+AGG + chr4_3:5175541-5175560 None:intergenic 30.0%
!! TTTTGAGTGAACACTCATTT+TGG - chr4_3:5175932-5175951 Msa0625840:intron 30.0%
TGAATGTATCCTCTATTAGT+TGG + chr4_3:5176056-5176075 None:intergenic 30.0%
!! ATTTTGGTCCTTAAATGTGT+CGG + chr4_3:5176127-5176146 None:intergenic 30.0%
!!! GAAAGGAGTAAGTGTTATTT+TGG + chr4_3:5176143-5176162 None:intergenic 30.0%
! TGTTTGGTAGTAATGTAGAA+AGG + chr4_3:5176165-5176184 None:intergenic 30.0%
ACATCACAGTTTCATCAAAA+CGG + chr4_3:5176411-5176430 None:intergenic 30.0%
!! CACTTTTGAAGATACAATGA+CGG + chr4_3:5176487-5176506 None:intergenic 30.0%
TAAGGCTCAATGAGAATATT+CGG + chr4_3:5176521-5176540 None:intergenic 30.0%
GCTCCATTGAAAAACAATTA+AGG - chr4_3:5177206-5177225 Msa0625840:five_prime_UTR 30.0%
! AAGGGATGTTGCTAATTTTA+AGG - chr4_3:5177225-5177244 Msa0625840:five_prime_UTR 30.0%
TTGATCCTTTGTCGAAAAGA+GGG + chr4_3:5173765-5173784 None:intergenic 35.0%
TTTGATCCTTTGTCGAAAAG+AGG + chr4_3:5173766-5173785 None:intergenic 35.0%
!! ATGGGTATTTTTGCTGCTAT+TGG - chr4_3:5173862-5173881 Msa0625840:three_prime_UTR 35.0%
AAGAGACCCTTCAAAGATTA+TGG - chr4_3:5173960-5173979 Msa0625840:three_prime_UTR 35.0%
AAAAGGGGCCAAAAGTAAAA+AGG + chr4_3:5174060-5174079 None:intergenic 35.0%
AACTGTCCAAATGCAAAAAG+GGG + chr4_3:5174075-5174094 None:intergenic 35.0%
GAACTGTCCAAATGCAAAAA+GGG + chr4_3:5174076-5174095 None:intergenic 35.0%
!!! CTGGGTTTTGTTTGAATTTG+TGG - chr4_3:5174143-5174162 Msa0625840:CDS 35.0%
! AATGCTATATGGTGCTGAAT+TGG - chr4_3:5174217-5174236 Msa0625840:CDS 35.0%
! TCAATGCTTGGATTCACTTT+TGG - chr4_3:5174554-5174573 Msa0625840:intron 35.0%
! AGTTGTGTTTGGTTCAAATG+GGG + chr4_3:5174801-5174820 None:intergenic 35.0%
ATGGTGTATCACAATGATTG+TGG + chr4_3:5174951-5174970 None:intergenic 35.0%
TCTCAATTCGATATGCTAAC+TGG - chr4_3:5175076-5175095 Msa0625840:intron 35.0%
AGTAGTGAGTAGCGAATAAT+GGG - chr4_3:5175208-5175227 Msa0625840:intron 35.0%
!!! AGTTGATTTTTGGCATGTTC+AGG - chr4_3:5175329-5175348 Msa0625840:intron 35.0%
!!! GTTGATTTTTGGCATGTTCA+GGG - chr4_3:5175330-5175349 Msa0625840:intron 35.0%
AGGCGAAATCAATTCGAATT+AGG + chr4_3:5175358-5175377 None:intergenic 35.0%
AATGATCGCAGCTTGAAATT+AGG - chr4_3:5175382-5175401 Msa0625840:intron 35.0%
ATTATACACCACAGCAAAGT+GGG + chr4_3:5175637-5175656 None:intergenic 35.0%
AATTATACACCACAGCAAAG+TGG + chr4_3:5175638-5175657 None:intergenic 35.0%
TCTCAATTTGATATGCCGAT+TGG - chr4_3:5175717-5175736 Msa0625840:intron 35.0%
ATTTACACCATTGCACCAAT+CGG + chr4_3:5175735-5175754 None:intergenic 35.0%
! ATCCGTATTTTATGGGTTCT+TGG - chr4_3:5175798-5175817 Msa0625840:intron 35.0%
CACCAAGAACCCATAAAATA+CGG + chr4_3:5175803-5175822 None:intergenic 35.0%
GAGAATATCACAATTGCAGA+AGG - chr4_3:5175890-5175909 Msa0625840:intron 35.0%
! TATCCTCTATTAGTTGGCTT+GGG + chr4_3:5176050-5176069 None:intergenic 35.0%
! TTTTGGTCCTTAAATGTGTC+GGG + chr4_3:5176126-5176145 None:intergenic 35.0%
GGTAGTAATGTAGAAAGGAA+AGG + chr4_3:5176160-5176179 None:intergenic 35.0%
GCTTTCTTGGTAAGATTGTT+TGG + chr4_3:5176181-5176200 None:intergenic 35.0%
!! TTTTGAAGATACAATGACGG+AGG + chr4_3:5176484-5176503 None:intergenic 35.0%
AAGGCTCAATGAGAATATTC+GGG + chr4_3:5176520-5176539 None:intergenic 35.0%
TGATCACATTGTCAAATGAC+AGG - chr4_3:5176581-5176600 Msa0625840:intron 35.0%
GATCACATTGTCAAATGACA+GGG - chr4_3:5176582-5176601 Msa0625840:intron 35.0%
! CTTTGGAAAAACTCTTCCAT+GGG - chr4_3:5176648-5176667 Msa0625840:intron 35.0%
TCTTGTTGCATGCATTTCAT+GGG - chr4_3:5176729-5176748 Msa0625840:intron 35.0%
ACACATATCCTAAAATCCAC+AGG + chr4_3:5176774-5176793 None:intergenic 35.0%
ATATGTGTTTATGATGCACG+TGG - chr4_3:5176786-5176805 Msa0625840:intron 35.0%
! CAATTGATTCAGCATGTGTT+TGG - chr4_3:5176846-5176865 Msa0625840:intron 35.0%
!! ACTGAACTTGATAGCTTGTT+TGG + chr4_3:5176987-5177006 None:intergenic 35.0%
AACATAATAGGACTCTCCAT+TGG - chr4_3:5177248-5177267 Msa0625840:five_prime_UTR 35.0%
! TGTAACCCTCTTTTCGACAA+AGG - chr4_3:5173757-5173776 Msa0625840:three_prime_UTR 40.0%
ATGATTGTGGCCAATTCCAA+AGG + chr4_3:5173918-5173937 None:intergenic 40.0%
! TTTGGAATTGGCCACAATCA+TGG - chr4_3:5173917-5173936 Msa0625840:three_prime_UTR 40.0%
TTGGAATTGGCCACAATCAT+GGG - chr4_3:5173918-5173937 Msa0625840:three_prime_UTR 40.0%
CAGAAATCAACCCATGATTG+TGG + chr4_3:5173931-5173950 None:intergenic 40.0%
!! CAATCATGGGTTGATTTCTG+TGG - chr4_3:5173931-5173950 Msa0625840:three_prime_UTR 40.0%
!! TGGGTTGATTTCTGTGGTAA+AGG - chr4_3:5173937-5173956 Msa0625840:three_prime_UTR 40.0%
! TTTTGGCCCCTTTTTGCATT+TGG - chr4_3:5174066-5174085 Msa0625840:three_prime_UTR 40.0%
GGAACTGTCCAAATGCAAAA+AGG + chr4_3:5174077-5174096 None:intergenic 40.0%
TTGGACAGTTCCTTAACTTC+AGG - chr4_3:5174085-5174104 Msa0625840:three_prime_UTR 40.0%
! AAGGAGATTTAGGGTCTGTT+TGG - chr4_3:5174319-5174338 Msa0625840:CDS 40.0%
TGACTTAAGCCTGTTTGGAT+TGG + chr4_3:5174537-5174556 None:intergenic 40.0%
!! AGCATTGACTTAAGCCTGTT+TGG + chr4_3:5174542-5174561 None:intergenic 40.0%
AACAGGCTTAAGTCAATGCT+TGG - chr4_3:5174542-5174561 Msa0625840:intron 40.0%
CAATTCGATATGCTAACTGG+TGG - chr4_3:5175079-5175098 Msa0625840:intron 40.0%
GATATCCTGATGCCATGATA+CGG - chr4_3:5175154-5175173 Msa0625840:intron 40.0%
TGCATAGAAACTCCGTATCA+TGG + chr4_3:5175169-5175188 None:intergenic 40.0%
GAGTAGTGAGTAGCGAATAA+TGG - chr4_3:5175207-5175226 Msa0625840:intron 40.0%
GTAGTGAGTAGCGAATAATG+GGG - chr4_3:5175209-5175228 Msa0625840:intron 40.0%
TCAAGCTGCGATCATTCTAA+AGG + chr4_3:5175378-5175397 None:intergenic 40.0%
TTATACACCACAGCAAAGTG+GGG + chr4_3:5175636-5175655 None:intergenic 40.0%
TGGTTCTTACGTGTATGAAG+TGG - chr4_3:5175952-5175971 Msa0625840:intron 40.0%
GTATCCTCTATTAGTTGGCT+TGG + chr4_3:5176051-5176070 None:intergenic 40.0%
! TGATTTGCAGAATGAACTGC+AGG + chr4_3:5176302-5176321 None:intergenic 40.0%
CAAAACGGTGGTATTCATCT+TGG + chr4_3:5176396-5176415 None:intergenic 40.0%
TCACAGTTTCATCAAAACGG+TGG + chr4_3:5176408-5176427 None:intergenic 40.0%
!! GCTTTGGAAAAACTCTTCCA+TGG - chr4_3:5176647-5176666 Msa0625840:intron 40.0%
ATCAGTGATCCTCAGTATGT+CGG - chr4_3:5176694-5176713 Msa0625840:intron 40.0%
! GTCTTGTTGCATGCATTTCA+TGG - chr4_3:5176728-5176747 Msa0625840:intron 40.0%
! TACATCCTCCTGTGGATTTT+AGG - chr4_3:5176763-5176782 Msa0625840:intron 40.0%
CATATCCTAAAATCCACAGG+AGG + chr4_3:5176771-5176790 None:intergenic 40.0%
CACATGCTGAATCAATTGAG+TGG + chr4_3:5176844-5176863 None:intergenic 40.0%
CAACAAGAAGCTACATCTTG+TGG + chr4_3:5176932-5176951 None:intergenic 40.0%
CACAAGATGTAGCTTCTTGT+TGG - chr4_3:5176930-5176949 Msa0625840:CDS 40.0%
!! TGATAGCTTGTTTGGAACCA+CGG + chr4_3:5176979-5176998 None:intergenic 40.0%
!! TTCAATGGAGCTTGATGATG+TGG + chr4_3:5177197-5177216 None:intergenic 40.0%
! TACATTTAGAGCGTCTCCAA+TGG + chr4_3:5177267-5177286 None:intergenic 40.0%
! GGCCTCCTCCTTTTTACTTT+TGG - chr4_3:5174049-5174068 Msa0625840:three_prime_UTR 45.0%
GGCCAAAAGTAAAAAGGAGG+AGG + chr4_3:5174054-5174073 None:intergenic 45.0%
AGGGGCCAAAAGTAAAAAGG+AGG + chr4_3:5174057-5174076 None:intergenic 45.0%
! GCTATATGGTGCTGAATTGG+AGG - chr4_3:5174220-5174239 Msa0625840:CDS 45.0%
TCGATACATGCACGTGAAAG+TGG + chr4_3:5174713-5174732 None:intergenic 45.0%
AAACTCCGTATCATGGCATC+AGG + chr4_3:5175162-5175181 None:intergenic 45.0%
ATACTTTCCCCACTTTGCTG+TGG - chr4_3:5175626-5175645 Msa0625840:intron 45.0%
! TGATATGCCGATTGGTGCAA+TGG - chr4_3:5175725-5175744 Msa0625840:intron 45.0%
TGTGATATTCTCGCAGCCAA+AGG + chr4_3:5175882-5175901 None:intergenic 45.0%
TGACCCAAGCCAACTAATAG+AGG - chr4_3:5176044-5176063 Msa0625840:intron 45.0%
GTTAGTGCCCGACACATTTA+AGG - chr4_3:5176116-5176135 Msa0625840:intron 45.0%
!! ATCTTACCAAGAAAGCACCC+TGG - chr4_3:5176185-5176204 Msa0625840:intron 45.0%
!! TGTTTTCCAGGGTGCTTTCT+TGG + chr4_3:5176194-5176213 None:intergenic 45.0%
AAAGGGAATTCGGTTACCCA+TGG + chr4_3:5176667-5176686 None:intergenic 45.0%
TGGGTAACCGAATTCCCTTT+CGG - chr4_3:5176667-5176686 Msa0625840:intron 45.0%
CACAGGAGGATGTACTGAAA+AGG + chr4_3:5176757-5176776 None:intergenic 45.0%
! CTTTTCAGTACATCCTCCTG+TGG - chr4_3:5176755-5176774 Msa0625840:intron 45.0%
GCTGAATCAATTGAGTGGCT+TGG + chr4_3:5176839-5176858 None:intergenic 45.0%
CTCGAGACGCAAAAATCAAG+AGG + chr4_3:5176883-5176902 None:intergenic 45.0%
! CTCTTGATTTTTGCGTCTCG+AGG - chr4_3:5176881-5176900 Msa0625840:CDS 45.0%
TTCTTGTTGGACGCGAGTTT+AGG - chr4_3:5176943-5176962 Msa0625840:CDS 45.0%
GACAGGGTTTATCAGCTGCT+TGG - chr4_3:5176598-5176617 Msa0625840:intron 50.0%
! TATCAGCTGCTTGGTGAACC+CGG - chr4_3:5176607-5176626 Msa0625840:intron 50.0%
ACGTACTACGGTGTACGCTT+TGG - chr4_3:5176631-5176650 Msa0625840:intron 50.0%
GATGACGCCGAAAGGGAATT+CGG + chr4_3:5176677-5176696 None:intergenic 50.0%
! GATCACTGATGACGCCGAAA+GGG + chr4_3:5176684-5176703 None:intergenic 50.0%
ATGATGCTGCCGACATACTG+AGG + chr4_3:5176706-5176725 None:intergenic 50.0%
TATGATGCACGTGGAGTCGA+AGG - chr4_3:5176795-5176814 Msa0625840:intron 50.0%
ACGCGAGTTTAGGCCAAATG+TGG - chr4_3:5176953-5176972 Msa0625840:CDS 50.0%
! TTTGGAACCACGGCCACATT+TGG + chr4_3:5176969-5176988 None:intergenic 50.0%
TGCAGGTAGCAGTCTCTTGC+TGG + chr4_3:5176285-5176304 None:intergenic 55.0%
! GGATCACTGATGACGCCGAA+AGG + chr4_3:5176685-5176704 None:intergenic 55.0%
GTTTAGGCCAAATGTGGCCG+TGG - chr4_3:5176959-5176978 Msa0625840:CDS 55.0%
GGTGAACCCGGCACGTACTA+CGG - chr4_3:5176619-5176638 Msa0625840:intron 60.0%
CGTACACCGTAGTACGTGCC+GGG + chr4_3:5176628-5176647 None:intergenic 60.0%
GCGTACACCGTAGTACGTGC+CGG + chr4_3:5176629-5176648 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 5173677 5177295 5173677 ID=Msa0625840;Name=Msa0625840
chr4_3 mRNA 5173677 5177295 5173677 ID=Msa0625840-mRNA-1;Parent=Msa0625840;Name=Msa0625840-mRNA-1;_AED=0.16;_eAED=0.16;_QI=185|1|1|1|1|1|2|443|164
chr4_3 exon 5173677 5174369 5173677 ID=Msa0625840-mRNA-1:exon:1427;Parent=Msa0625840-mRNA-1
chr4_3 exon 5176866 5177295 5176866 ID=Msa0625840-mRNA-1:exon:1426;Parent=Msa0625840-mRNA-1
chr4_3 five_prime_UTR 5177111 5177295 5177111 ID=Msa0625840-mRNA-1:five_prime_utr;Parent=Msa0625840-mRNA-1
chr4_3 CDS 5176866 5177110 5176866 ID=Msa0625840-mRNA-1:cds;Parent=Msa0625840-mRNA-1
chr4_3 CDS 5174120 5174369 5174120 ID=Msa0625840-mRNA-1:cds;Parent=Msa0625840-mRNA-1
chr4_3 three_prime_UTR 5173677 5174119 5173677 ID=Msa0625840-mRNA-1:three_prime_utr;Parent=Msa0625840-mRNA-1
Gene Sequence

>Msa0625840

ATGGGTATTTTTGCTGCTATTGGAGTGTTATCACCATTTCCATTCTACTATTACCTTTGGAATTGGCCACAATCATGGGTTGATTTCTGTGGTAAAGGAAGAGACCCTTCAAAGATTATGGCTTATGTTGCTCATTTCTTGAAGATAATTCAGTTCATTTCACTCTTTTCTGTCTCTTCTTTTCATTGGCCTCCTCCTTTTTACTTTTGGCCCCTTTTTGCATTTGGACAGTTCCTTAACTTCAGGGTTTATCAGCTGCTTGGTGAACCCGGCACGTACTACGGTGTACGCTTTGGAAAAACTCTTCCATGGGTAACCGAATTCCCTTTCGGCGTCATCAGTGATCCTCAGTATGTCGGCAGCATCATGAGTCTTGTTGCATGCATTTCATGGGTTCCTTTTCAGTACATCCTCCTGTGGATTTTAGGATATGTGTTTATGATGCACGTGGAGTCGAAGGAAGATCAATCAACTCGAGCCAAGCCACTCAATTGA
Protein sequence

>Msa0625840

MGIFAAIGVLSPFPFYYYLWNWPQSWVDFCGKGRDPSKIMAYVAHFLKIIQFISLFSVSSFHWPPPFYFWPLFAFGQFLNFRVYQLLGEPGTYYGVRFGKTLPWVTEFPFGVISDPQYVGSIMSLVACISWVPFQYILLWILGYVFMMHVESKEDQSTRAKPLN*