AlfalfaGEDB | Msa0591400

M. sativa cultivar zhongmu-4 / Msa0591400

KEGG: K03264
GO: GO:0000003 GO:0000054 GO:0000460 GO:0000470 GO:0001101 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016072 GO:0022414 GO:0022607 GO:0022613 GO:0022618 GO:0030684 GO:0030687 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032870 GO:0032991 GO:0033036 GO:0033750 GO:0033993 GO:0034470 GO:0034613 GO:0034622 GO:0034641 GO:0034660 GO:0042221 GO:0042254 GO:0042273 GO:0042886 GO:0043021 GO:0043023 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050896 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0051716 GO:0061458 GO:0065003 GO:0070013 GO:0070727 GO:0070887 GO:0071166 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071426 GO:0071428 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0071826 GO:0071840 GO:0090304 GO:0097305 GO:0097306 GO:1901360 GO:1901700 GO:1901701 GO:1902626 GO:1990904
PFAM: PF01912.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591400	XP_003604849.1	76.543	81	7	1	12	80	5	85	3.64e-30	117

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591400	sp\|Q9M060\|IF62_ARATH	55.000	80	25	1	12	80	5	84	2.03e-22	89.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591400	G7JSA7	76.543	81	7	1	12	80	5	85	1.74e-30	117

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0205510	Msa0591400	0.807646	4.565773e-50	-8.615850e-47
Msa0466390	Msa0591400	0.801493	8.773483e-49	-8.615850e-47
Msa0315290	Msa0591400	0.812041	5.174850e-51	-8.615850e-47
Msa0536220	Msa0591400	0.831547	1.569214e-55	-8.615850e-47
Msa0591400	Msa1451620	-0.801304	9.592861e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591400	MtrunA17_Chr4g0008971	52.756	127	7	2	7	80	1	127	7.87e-30	106
Msa0591400	MtrunA17_Chr1g0188551	58.750	80	22	1	12	80	5	84	4.00e-25	94.0
Msa0591400	MtrunA17_Chr1g0188541	57.500	80	23	1	12	80	5	84	2.51e-23	89.4
Msa0591400	MtrunA17_Chr6g0477401	48.750	80	30	1	12	80	5	84	1.70e-19	79.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0591400	AT3G55620.1	55.000	80	25	1	12	80	5	84	2.07e-23	89.7
Msa0591400	AT2G39820.1	46.341	82	31	3	12	80	5	86	1.21e-13	64.3

Find 37 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAAACCTGTAGAAGTTAT+TGG	0.207613	4_2:-15631993	None:intergenic
TTTGACGACAGCACTGAAAT+TGG	0.236025	4_2:+15631920	Msa0591400:CDS
ACTCCTATGTACCACATATT+TGG	0.300196	4_2:+15633155	Msa0591400:three_prime_UTR
CACCTTCGAGTGTGGTTGTA+TGG	0.328182	4_2:-15632569	None:intergenic
GATGGCGCCATGGTGCTTTA+TGG	0.335060	4_2:+15633060	Msa0591400:three_prime_UTR
TGGCGCCATCCTTCACAAAA+TGG	0.346609	4_2:-15633047	None:intergenic
GCCTATTGCTTGGTTGGCAC+TGG	0.370534	4_2:+15631965	Msa0591400:CDS
TGTATAATTGTTCACCTCTC+TGG	0.386106	4_2:-15633249	None:intergenic
GCGTTGCCATGGAGGATTGT+GGG	0.387581	4_2:+15632999	Msa0591400:CDS
GTGCTGTCAAATATCTGTTA+TGG	0.425670	4_2:+15632977	Msa0591400:intron
GAGGTTCCAATAACTTCTAC+AGG	0.446983	4_2:+15631987	Msa0591400:CDS
ACCTTCGAGTGTGGTTGTAT+GGG	0.453764	4_2:-15632568	None:intergenic
TCCAGTGCCAACCAAGCAAT+AGG	0.468620	4_2:-15631966	None:intergenic
ACCAATGCCTATTGCTTGGT+TGG	0.469601	4_2:+15631959	Msa0591400:CDS
TTACCAAATATGTGGTACAT+AGG	0.470414	4_2:-15633158	None:intergenic
TATTGCTTGGTTGGCACTGG+AGG	0.513975	4_2:+15631968	Msa0591400:CDS
TCGTCAAACATAAGTCCTAC+AGG	0.523522	4_2:-15631905	None:intergenic
ACTCACCAATGCCTATTGCT+TGG	0.530940	4_2:+15631955	Msa0591400:CDS
GGCGTTGCCATGGAGGATTG+TGG	0.531864	4_2:+15632998	Msa0591400:CDS
CGTTGCCATGGAGGATTGTG+GGG	0.532448	4_2:+15633000	Msa0591400:CDS
GCCAACCAAGCAATAGGCAT+TGG	0.568365	4_2:-15631960	None:intergenic
GGCGCCATGGTGCTTTATGG+TGG	0.580629	4_2:+15633063	Msa0591400:three_prime_UTR
CCTTCGAGTGTGGTTGTATG+GGG	0.585052	4_2:-15632567	None:intergenic
CTGTTATGGCGTTGCCATGG+AGG	0.585152	4_2:+15632991	Msa0591400:CDS
CCTATCATCAAAACCTCCGT+TGG	0.591023	4_2:+15632139	Msa0591400:CDS
TATCTGTTATGGCGTTGCCA+TGG	0.598011	4_2:+15632988	Msa0591400:CDS
CCATCCTTCACAAAATGGCA+TGG	0.599834	4_2:-15633042	None:intergenic
CAATGGTATATGTCTCCTGT+AGG	0.600639	4_2:+15631890	Msa0591400:CDS
CCCCATACAACCACACTCGA+AGG	0.604581	4_2:+15632567	Msa0591400:CDS
GTTGCCATGGAGGATTGTGG+GGG	0.610371	4_2:+15633001	Msa0591400:CDS
GCTGAAATCACCTTCGAGTG+TGG	0.638684	4_2:-15632577	None:intergenic
GCTTTCAATAGTGTTGTCGA+TGG	0.643781	4_2:-15633101	None:intergenic
TTGCTACAATGAACACACAA+TGG	0.664922	4_2:+15631873	None:intergenic
TAGTGTTATTAGCACCAGAG+AGG	0.677823	4_2:+15633235	Msa0591400:three_prime_UTR
AAATCCACCATAAAGCACCA+TGG	0.697271	4_2:-15633067	None:intergenic
GTACATAGGAGTGACTTAGT+CGG	0.699352	4_2:-15633144	None:intergenic
ACCAGAACAGCCACCAACGG+AGG	0.715006	4_2:-15632152	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTAGATATTACAATAAAAA+GGG	+	chr4_2:15632442-15632461	Msa0591400:intron	10.0%
!!!	AAAAAATATTTTAAACAATC+TGG	-	chr4_2:15633332-15633351	None:intergenic	10.0%
!!	TTTAGATATTACAATAAAAA+GGG	+	chr4_2:15632442-15632461	Msa0591400:intron	10.0%
!!!	AAAAAATATTTTAAACAATC+TGG	-	chr4_2:15633332-15633351	None:intergenic	10.0%
!!	TAATGAAATATATTTCCTAT+TGG	+	chr4_2:15632030-15632049	Msa0591400:intron	15.0%
!!	ATATAAGAATATTACATTGT+TGG	-	chr4_2:15632317-15632336	None:intergenic	15.0%
!!!	ATTATATGCAAATTTGTTTA+AGG	+	chr4_2:15632364-15632383	Msa0591400:intron	15.0%
!!!	TTATATGCAAATTTGTTTAA+GGG	+	chr4_2:15632365-15632384	Msa0591400:intron	15.0%
!!	ATATCTAAACTATATGTAAA+GGG	-	chr4_2:15632431-15632450	None:intergenic	15.0%
!!	AATATCTAAACTATATGTAA+AGG	-	chr4_2:15632432-15632451	None:intergenic	15.0%
!!	GTTTAGATATTACAATAAAA+AGG	+	chr4_2:15632441-15632460	Msa0591400:intron	15.0%
!!!	ATTACTCTCTTATTTTATAA+AGG	+	chr4_2:15632831-15632850	Msa0591400:intron	15.0%
!!!	TCCATGTTTTTTTTTTTAAT+GGG	+	chr4_2:15632917-15632936	Msa0591400:intron	15.0%
!!	TAATGAAATATATTTCCTAT+TGG	+	chr4_2:15632030-15632049	Msa0591400:intron	15.0%
!!	ATATAAGAATATTACATTGT+TGG	-	chr4_2:15632317-15632336	None:intergenic	15.0%
!!!	ATTATATGCAAATTTGTTTA+AGG	+	chr4_2:15632364-15632383	Msa0591400:intron	15.0%
!!!	TTATATGCAAATTTGTTTAA+GGG	+	chr4_2:15632365-15632384	Msa0591400:intron	15.0%
!!	ATATCTAAACTATATGTAAA+GGG	-	chr4_2:15632431-15632450	None:intergenic	15.0%
!!	AATATCTAAACTATATGTAA+AGG	-	chr4_2:15632432-15632451	None:intergenic	15.0%
!!	GTTTAGATATTACAATAAAA+AGG	+	chr4_2:15632441-15632460	Msa0591400:intron	15.0%
!!!	ATTACTCTCTTATTTTATAA+AGG	+	chr4_2:15632831-15632850	Msa0591400:intron	15.0%
!!!	TCCATGTTTTTTTTTTTAAT+GGG	+	chr4_2:15632917-15632936	Msa0591400:intron	15.0%
!!!	AATTTGTTTAAGGGTATAAT+TGG	+	chr4_2:15632374-15632393	Msa0591400:intron	20.0%
!!	GCATAAACAAAACAATAAAT+AGG	-	chr4_2:15632484-15632503	None:intergenic	20.0%
!!!	TATTGTTTTGTTTATGCTAA+TGG	+	chr4_2:15632487-15632506	Msa0591400:intron	20.0%
!!!	ATTGTTTTGTTTATGCTAAT+GGG	+	chr4_2:15632488-15632507	Msa0591400:intron	20.0%
!!	AATAGAAAGGTAAACTTATA+AGG	-	chr4_2:15632515-15632534	None:intergenic	20.0%
!!	TGTACTAAGAATAAACTAAA+TGG	-	chr4_2:15632614-15632633	None:intergenic	20.0%
!!	TTGTAGATAGTAAAATACAT+AGG	+	chr4_2:15632795-15632814	Msa0591400:intron	20.0%
!!	TGTAGATAGTAAAATACATA+GGG	+	chr4_2:15632796-15632815	Msa0591400:intron	20.0%
!!!	GTCCATGTTTTTTTTTTTAA+TGG	+	chr4_2:15632916-15632935	Msa0591400:intron	20.0%
!!	TCCCATTAAAAAAAAAAACA+TGG	-	chr4_2:15632921-15632940	None:intergenic	20.0%
!!!	AATTTGTTTAAGGGTATAAT+TGG	+	chr4_2:15632374-15632393	Msa0591400:intron	20.0%
!!	GCATAAACAAAACAATAAAT+AGG	-	chr4_2:15632484-15632503	None:intergenic	20.0%
!!!	TATTGTTTTGTTTATGCTAA+TGG	+	chr4_2:15632487-15632506	Msa0591400:intron	20.0%
!!!	ATTGTTTTGTTTATGCTAAT+GGG	+	chr4_2:15632488-15632507	Msa0591400:intron	20.0%
!!	AATAGAAAGGTAAACTTATA+AGG	-	chr4_2:15632515-15632534	None:intergenic	20.0%
!!	TGTACTAAGAATAAACTAAA+TGG	-	chr4_2:15632614-15632633	None:intergenic	20.0%
!!	TTGTAGATAGTAAAATACAT+AGG	+	chr4_2:15632795-15632814	Msa0591400:intron	20.0%
!!	TGTAGATAGTAAAATACATA+GGG	+	chr4_2:15632796-15632815	Msa0591400:intron	20.0%
!!!	GTCCATGTTTTTTTTTTTAA+TGG	+	chr4_2:15632916-15632935	Msa0591400:intron	20.0%
!!	TCCCATTAAAAAAAAAAACA+TGG	-	chr4_2:15632921-15632940	None:intergenic	20.0%
!!!	ATTTTGCATTTGGTTTCAAA+TGG	+	chr4_2:15632054-15632073	Msa0591400:intron	25.0%
!!	GGAATAAATGTATGTGTTTT+TGG	+	chr4_2:15632080-15632099	Msa0591400:intron	25.0%
!	AATGCTTATGTTAATGTAGT+GGG	+	chr4_2:15632638-15632657	Msa0591400:intron	25.0%
!	GCTATTTAAAAGAACAGTTT+AGG	-	chr4_2:15632740-15632759	None:intergenic	25.0%
!!	ATTTTACTATCTACAATGCT+GGG	-	chr4_2:15632791-15632810	None:intergenic	25.0%
!!	TATTTTACTATCTACAATGC+TGG	-	chr4_2:15632792-15632811	None:intergenic	25.0%
!!	GGTAATTTTTACCAAATATG+TGG	-	chr4_2:15633169-15633188	None:intergenic	25.0%
!	AAAAGCCATAAAAATAGTCT+AGG	-	chr4_2:15633278-15633297	None:intergenic	25.0%
!!!	TAGACTATTTTTATGGCTTT+TGG	+	chr4_2:15633277-15633296	Msa0591400:three_prime_UTR	25.0%
!!!	ATTTTGCATTTGGTTTCAAA+TGG	+	chr4_2:15632054-15632073	Msa0591400:intron	25.0%
!!	GGAATAAATGTATGTGTTTT+TGG	+	chr4_2:15632080-15632099	Msa0591400:intron	25.0%
!	AATGCTTATGTTAATGTAGT+GGG	+	chr4_2:15632638-15632657	Msa0591400:intron	25.0%
!	GCTATTTAAAAGAACAGTTT+AGG	-	chr4_2:15632740-15632759	None:intergenic	25.0%
!!	ATTTTACTATCTACAATGCT+GGG	-	chr4_2:15632791-15632810	None:intergenic	25.0%
!!	TATTTTACTATCTACAATGC+TGG	-	chr4_2:15632792-15632811	None:intergenic	25.0%
!!	GGTAATTTTTACCAAATATG+TGG	-	chr4_2:15633169-15633188	None:intergenic	25.0%
!	AAAAGCCATAAAAATAGTCT+AGG	-	chr4_2:15633278-15633297	None:intergenic	25.0%
!!!	TAGACTATTTTTATGGCTTT+TGG	+	chr4_2:15633277-15633296	Msa0591400:three_prime_UTR	25.0%
	TGAAAACCTGTAGAAGTTAT+TGG	-	chr4_2:15631996-15632015	None:intergenic	30.0%
!	TCCTATTGGAATTTTGCATT+TGG	+	chr4_2:15632044-15632063	Msa0591400:intron	30.0%
	ACCAAATGCAAAATTCCAAT+AGG	-	chr4_2:15632048-15632067	None:intergenic	30.0%
!	TATTTAAAACCCCACAACTT+CGG	-	chr4_2:15632225-15632244	None:intergenic	30.0%
!!!	GTTGTGGGGTTTTAAATAAA+AGG	+	chr4_2:15632227-15632246	Msa0591400:intron	30.0%
!	TAAAGGGAATACATGGTTTT+GGG	-	chr4_2:15632415-15632434	None:intergenic	30.0%
	CTATATGTAAAGGGAATACA+TGG	-	chr4_2:15632422-15632441	None:intergenic	30.0%
!!	TTACCTTTCTATTGTTTTGC+AGG	+	chr4_2:15632522-15632541	Msa0591400:intron	30.0%
	TTTCCTGCAAAACAATAGAA+AGG	-	chr4_2:15632528-15632547	None:intergenic	30.0%
!	TGTTTTGCAGGAAACAAAAA+TGG	+	chr4_2:15632534-15632553	Msa0591400:intron	30.0%
!	GTTTTGCAGGAAACAAAAAT+GGG	+	chr4_2:15632535-15632554	Msa0591400:intron	30.0%
	GAATGCTTATGTTAATGTAG+TGG	+	chr4_2:15632637-15632656	Msa0591400:intron	30.0%
	CTTTAGTGACCTAAACAATT+GGG	-	chr4_2:15632715-15632734	None:intergenic	30.0%
	TCTTTAGTGACCTAAACAAT+TGG	-	chr4_2:15632716-15632735	None:intergenic	30.0%
	TTACCAAATATGTGGTACAT+AGG	-	chr4_2:15633161-15633180	None:intergenic	30.0%
	TTCTTCAGTGCAAGTAAAAA+TGG	-	chr4_2:15633190-15633209	None:intergenic	30.0%
!!	ACTGAAGAATATTTTGAGCT+AGG	+	chr4_2:15633201-15633220	Msa0591400:three_prime_UTR	30.0%
!!	TATTTTGAGCTAGGAAACTT+TGG	+	chr4_2:15633210-15633229	Msa0591400:three_prime_UTR	30.0%
!!!	CACTACCTAGACTATTTTTA+TGG	+	chr4_2:15633270-15633289	Msa0591400:three_prime_UTR	30.0%
	TGAAAACCTGTAGAAGTTAT+TGG	-	chr4_2:15631996-15632015	None:intergenic	30.0%
!	TCCTATTGGAATTTTGCATT+TGG	+	chr4_2:15632044-15632063	Msa0591400:intron	30.0%
	ACCAAATGCAAAATTCCAAT+AGG	-	chr4_2:15632048-15632067	None:intergenic	30.0%
!	TATTTAAAACCCCACAACTT+CGG	-	chr4_2:15632225-15632244	None:intergenic	30.0%
!!!	GTTGTGGGGTTTTAAATAAA+AGG	+	chr4_2:15632227-15632246	Msa0591400:intron	30.0%
!	TAAAGGGAATACATGGTTTT+GGG	-	chr4_2:15632415-15632434	None:intergenic	30.0%
	CTATATGTAAAGGGAATACA+TGG	-	chr4_2:15632422-15632441	None:intergenic	30.0%
!!	TTACCTTTCTATTGTTTTGC+AGG	+	chr4_2:15632522-15632541	Msa0591400:intron	30.0%
	TTTCCTGCAAAACAATAGAA+AGG	-	chr4_2:15632528-15632547	None:intergenic	30.0%
!	TGTTTTGCAGGAAACAAAAA+TGG	+	chr4_2:15632534-15632553	Msa0591400:intron	30.0%
!	GTTTTGCAGGAAACAAAAAT+GGG	+	chr4_2:15632535-15632554	Msa0591400:intron	30.0%
	GAATGCTTATGTTAATGTAG+TGG	+	chr4_2:15632637-15632656	Msa0591400:intron	30.0%
	CTTTAGTGACCTAAACAATT+GGG	-	chr4_2:15632715-15632734	None:intergenic	30.0%
	TCTTTAGTGACCTAAACAAT+TGG	-	chr4_2:15632716-15632735	None:intergenic	30.0%
	TTACCAAATATGTGGTACAT+AGG	-	chr4_2:15633161-15633180	None:intergenic	30.0%
	TTCTTCAGTGCAAGTAAAAA+TGG	-	chr4_2:15633190-15633209	None:intergenic	30.0%
!!	ACTGAAGAATATTTTGAGCT+AGG	+	chr4_2:15633201-15633220	Msa0591400:three_prime_UTR	30.0%
!!	TATTTTGAGCTAGGAAACTT+TGG	+	chr4_2:15633210-15633229	Msa0591400:three_prime_UTR	30.0%
!!!	CACTACCTAGACTATTTTTA+TGG	+	chr4_2:15633270-15633289	Msa0591400:three_prime_UTR	30.0%
!	GCATTTGGTTTCAAATGGAT+TGG	+	chr4_2:15632059-15632078	Msa0591400:intron	35.0%
!!	TTTTTGGTACAGTGCAATTG+AGG	+	chr4_2:15632096-15632115	Msa0591400:intron	35.0%
!	GTAAAGGGAATACATGGTTT+TGG	-	chr4_2:15632416-15632435	None:intergenic	35.0%
	AACAACTCACCCAATTGTTT+AGG	+	chr4_2:15632703-15632722	Msa0591400:intron	35.0%
	AAGGAAAGTCAGTTAGTATG+AGG	+	chr4_2:15632850-15632869	Msa0591400:intron	35.0%
!!	GTTCTCTTGGTAATGATTGA+TGG	+	chr4_2:15632872-15632891	Msa0591400:intron	35.0%
	GTGCTGTCAAATATCTGTTA+TGG	+	chr4_2:15632977-15632996	Msa0591400:intron	35.0%
	ACTCCTATGTACCACATATT+TGG	+	chr4_2:15633155-15633174	Msa0591400:three_prime_UTR	35.0%
	TGTATAATTGTTCACCTCTC+TGG	-	chr4_2:15633252-15633271	None:intergenic	35.0%
!	GCATTTGGTTTCAAATGGAT+TGG	+	chr4_2:15632059-15632078	Msa0591400:intron	35.0%
!!	TTTTTGGTACAGTGCAATTG+AGG	+	chr4_2:15632096-15632115	Msa0591400:intron	35.0%
!	GTAAAGGGAATACATGGTTT+TGG	-	chr4_2:15632416-15632435	None:intergenic	35.0%
	AACAACTCACCCAATTGTTT+AGG	+	chr4_2:15632703-15632722	Msa0591400:intron	35.0%
	AAGGAAAGTCAGTTAGTATG+AGG	+	chr4_2:15632850-15632869	Msa0591400:intron	35.0%
!!	GTTCTCTTGGTAATGATTGA+TGG	+	chr4_2:15632872-15632891	Msa0591400:intron	35.0%
	GTGCTGTCAAATATCTGTTA+TGG	+	chr4_2:15632977-15632996	Msa0591400:intron	35.0%
	ACTCCTATGTACCACATATT+TGG	+	chr4_2:15633155-15633174	Msa0591400:three_prime_UTR	35.0%
	TGTATAATTGTTCACCTCTC+TGG	-	chr4_2:15633252-15633271	None:intergenic	35.0%
	CAATGGTATATGTCTCCTGT+AGG	+	chr4_2:15631890-15631909	Msa0591400:CDS	40.0%
	TCGTCAAACATAAGTCCTAC+AGG	-	chr4_2:15631908-15631927	None:intergenic	40.0%
!	TTTGACGACAGCACTGAAAT+TGG	+	chr4_2:15631920-15631939	Msa0591400:CDS	40.0%
	GAGGTTCCAATAACTTCTAC+AGG	+	chr4_2:15631987-15632006	Msa0591400:CDS	40.0%
!!	CAACGGAGGTTTTGATGATA+GGG	-	chr4_2:15632141-15632160	None:intergenic	40.0%
!	AATGAGTATTGCCGAAGTTG+TGG	+	chr4_2:15632211-15632230	Msa0591400:intron	40.0%
!	ATGAGTATTGCCGAAGTTGT+GGG	+	chr4_2:15632212-15632231	Msa0591400:intron	40.0%
!	CAATTGGGTGAGTTGTTTCA+CGG	-	chr4_2:15632700-15632719	None:intergenic	40.0%
	CTATCTACAATGCTGGGAAA+TGG	-	chr4_2:15632785-15632804	None:intergenic	40.0%
	CAGTTAGTATGAGGTTCTCT+TGG	+	chr4_2:15632859-15632878	Msa0591400:intron	40.0%
!	AAATCCACCATAAAGCACCA+TGG	-	chr4_2:15633070-15633089	None:intergenic	40.0%
!!!	GGTGCTTTATGGTGGATTTT+TGG	+	chr4_2:15633071-15633090	Msa0591400:three_prime_UTR	40.0%
	GCTTTCAATAGTGTTGTCGA+TGG	-	chr4_2:15633104-15633123	None:intergenic	40.0%
!!!	AGTCGGTGCTACGTTTTAAT+TGG	-	chr4_2:15633130-15633149	None:intergenic	40.0%
	GTACATAGGAGTGACTTAGT+CGG	-	chr4_2:15633147-15633166	None:intergenic	40.0%
!	TAGTGTTATTAGCACCAGAG+AGG	+	chr4_2:15633235-15633254	Msa0591400:three_prime_UTR	40.0%
	CAATGGTATATGTCTCCTGT+AGG	+	chr4_2:15631890-15631909	Msa0591400:CDS	40.0%
	TCGTCAAACATAAGTCCTAC+AGG	-	chr4_2:15631908-15631927	None:intergenic	40.0%
!	TTTGACGACAGCACTGAAAT+TGG	+	chr4_2:15631920-15631939	Msa0591400:CDS	40.0%
	GAGGTTCCAATAACTTCTAC+AGG	+	chr4_2:15631987-15632006	Msa0591400:CDS	40.0%
!!	CAACGGAGGTTTTGATGATA+GGG	-	chr4_2:15632141-15632160	None:intergenic	40.0%
!	AATGAGTATTGCCGAAGTTG+TGG	+	chr4_2:15632211-15632230	Msa0591400:intron	40.0%
!	ATGAGTATTGCCGAAGTTGT+GGG	+	chr4_2:15632212-15632231	Msa0591400:intron	40.0%
!	CAATTGGGTGAGTTGTTTCA+CGG	-	chr4_2:15632700-15632719	None:intergenic	40.0%
	CTATCTACAATGCTGGGAAA+TGG	-	chr4_2:15632785-15632804	None:intergenic	40.0%
	CAGTTAGTATGAGGTTCTCT+TGG	+	chr4_2:15632859-15632878	Msa0591400:intron	40.0%
!	AAATCCACCATAAAGCACCA+TGG	-	chr4_2:15633070-15633089	None:intergenic	40.0%
!!!	GGTGCTTTATGGTGGATTTT+TGG	+	chr4_2:15633071-15633090	Msa0591400:three_prime_UTR	40.0%
	GCTTTCAATAGTGTTGTCGA+TGG	-	chr4_2:15633104-15633123	None:intergenic	40.0%
!!!	AGTCGGTGCTACGTTTTAAT+TGG	-	chr4_2:15633130-15633149	None:intergenic	40.0%
	GTACATAGGAGTGACTTAGT+CGG	-	chr4_2:15633147-15633166	None:intergenic	40.0%
!	TAGTGTTATTAGCACCAGAG+AGG	+	chr4_2:15633235-15633254	Msa0591400:three_prime_UTR	40.0%
	ACTCACCAATGCCTATTGCT+TGG	+	chr4_2:15631955-15631974	Msa0591400:CDS	45.0%
	ACCAATGCCTATTGCTTGGT+TGG	+	chr4_2:15631959-15631978	Msa0591400:CDS	45.0%
!!	CCAACGGAGGTTTTGATGAT+AGG	-	chr4_2:15632142-15632161	None:intergenic	45.0%
	CCTATCATCAAAACCTCCGT+TGG	+	chr4_2:15632139-15632158	Msa0591400:CDS	45.0%
	ATCATCAAAACCTCCGTTGG+TGG	+	chr4_2:15632142-15632161	Msa0591400:intron	45.0%
!!!	GGTGTTGGTCGTTTTTGTGT+TGG	+	chr4_2:15632172-15632191	Msa0591400:intron	45.0%
!	TGAGTATTGCCGAAGTTGTG+GGG	+	chr4_2:15632213-15632232	Msa0591400:intron	45.0%
	ACCTTCGAGTGTGGTTGTAT+GGG	-	chr4_2:15632571-15632590	None:intergenic	45.0%
	CGAAGGTGATTTCAGCTGTT+AGG	+	chr4_2:15632584-15632603	Msa0591400:intron	45.0%
!	AGTTGTTTCACGGTGGAAAG+AGG	-	chr4_2:15632690-15632709	None:intergenic	45.0%
	TCTACAATGCTGGGAAATGG+AGG	-	chr4_2:15632782-15632801	None:intergenic	45.0%
	TATCTGTTATGGCGTTGCCA+TGG	+	chr4_2:15632988-15633007	Msa0591400:CDS	45.0%
!!!	ACTGCCATGCCATTTTGTGA+AGG	+	chr4_2:15633038-15633057	Msa0591400:three_prime_UTR	45.0%
	CCATCCTTCACAAAATGGCA+TGG	-	chr4_2:15633045-15633064	None:intergenic	45.0%
!!!	CCATGCCATTTTGTGAAGGA+TGG	+	chr4_2:15633042-15633061	Msa0591400:three_prime_UTR	45.0%
	ACTCACCAATGCCTATTGCT+TGG	+	chr4_2:15631955-15631974	Msa0591400:CDS	45.0%
	ACCAATGCCTATTGCTTGGT+TGG	+	chr4_2:15631959-15631978	Msa0591400:CDS	45.0%
!!	CCAACGGAGGTTTTGATGAT+AGG	-	chr4_2:15632142-15632161	None:intergenic	45.0%
	CCTATCATCAAAACCTCCGT+TGG	+	chr4_2:15632139-15632158	Msa0591400:CDS	45.0%
	ATCATCAAAACCTCCGTTGG+TGG	+	chr4_2:15632142-15632161	Msa0591400:intron	45.0%
!!!	GGTGTTGGTCGTTTTTGTGT+TGG	+	chr4_2:15632172-15632191	Msa0591400:intron	45.0%
!	TGAGTATTGCCGAAGTTGTG+GGG	+	chr4_2:15632213-15632232	Msa0591400:intron	45.0%
	ACCTTCGAGTGTGGTTGTAT+GGG	-	chr4_2:15632571-15632590	None:intergenic	45.0%
	CGAAGGTGATTTCAGCTGTT+AGG	+	chr4_2:15632584-15632603	Msa0591400:intron	45.0%
!	AGTTGTTTCACGGTGGAAAG+AGG	-	chr4_2:15632690-15632709	None:intergenic	45.0%
	TCTACAATGCTGGGAAATGG+AGG	-	chr4_2:15632782-15632801	None:intergenic	45.0%
	TATCTGTTATGGCGTTGCCA+TGG	+	chr4_2:15632988-15633007	Msa0591400:CDS	45.0%
!!!	ACTGCCATGCCATTTTGTGA+AGG	+	chr4_2:15633038-15633057	Msa0591400:three_prime_UTR	45.0%
	CCATCCTTCACAAAATGGCA+TGG	-	chr4_2:15633045-15633064	None:intergenic	45.0%
!!!	CCATGCCATTTTGTGAAGGA+TGG	+	chr4_2:15633042-15633061	Msa0591400:three_prime_UTR	45.0%
!!	CATATAATAATATATATTAT+TGG	-	chr4_2:15632352-15632371	None:intergenic	5.0%
!!!	TAAAATATTTTTTTAACAAA+AGG	+	chr4_2:15633339-15633358	Msa0591400:three_prime_UTR	5.0%
!!!	AAAATATTTTTTTAACAAAA+GGG	+	chr4_2:15633340-15633359	Msa0591400:three_prime_UTR	5.0%
!!	CATATAATAATATATATTAT+TGG	-	chr4_2:15632352-15632371	None:intergenic	5.0%
!!!	TAAAATATTTTTTTAACAAA+AGG	+	chr4_2:15633339-15633358	Msa0591400:three_prime_UTR	5.0%
!!!	AAAATATTTTTTTAACAAAA+GGG	+	chr4_2:15633340-15633359	Msa0591400:three_prime_UTR	5.0%
	GCCAACCAAGCAATAGGCAT+TGG	-	chr4_2:15631963-15631982	None:intergenic	50.0%
	TCCAGTGCCAACCAAGCAAT+AGG	-	chr4_2:15631969-15631988	None:intergenic	50.0%
!	TATTGCTTGGTTGGCACTGG+AGG	+	chr4_2:15631968-15631987	Msa0591400:CDS	50.0%
	AACACCAGAACAGCCACCAA+CGG	-	chr4_2:15632158-15632177	None:intergenic	50.0%
	CCTTCGAGTGTGGTTGTATG+GGG	-	chr4_2:15632570-15632589	None:intergenic	50.0%
	CACCTTCGAGTGTGGTTGTA+TGG	-	chr4_2:15632572-15632591	None:intergenic	50.0%
	GCTGAAATCACCTTCGAGTG+TGG	-	chr4_2:15632580-15632599	None:intergenic	50.0%
!	TTGGGTGAGTTGTTTCACGG+TGG	-	chr4_2:15632697-15632716	None:intergenic	50.0%
	TGGCGCCATCCTTCACAAAA+TGG	-	chr4_2:15633050-15633069	None:intergenic	50.0%
!	TTTTGTGAAGGATGGCGCCA+TGG	+	chr4_2:15633050-15633069	Msa0591400:three_prime_UTR	50.0%
	GCCAACCAAGCAATAGGCAT+TGG	-	chr4_2:15631963-15631982	None:intergenic	50.0%
	TCCAGTGCCAACCAAGCAAT+AGG	-	chr4_2:15631969-15631988	None:intergenic	50.0%
!	TATTGCTTGGTTGGCACTGG+AGG	+	chr4_2:15631968-15631987	Msa0591400:CDS	50.0%
	AACACCAGAACAGCCACCAA+CGG	-	chr4_2:15632158-15632177	None:intergenic	50.0%
	CCTTCGAGTGTGGTTGTATG+GGG	-	chr4_2:15632570-15632589	None:intergenic	50.0%
	CACCTTCGAGTGTGGTTGTA+TGG	-	chr4_2:15632572-15632591	None:intergenic	50.0%
	GCTGAAATCACCTTCGAGTG+TGG	-	chr4_2:15632580-15632599	None:intergenic	50.0%
!	TTGGGTGAGTTGTTTCACGG+TGG	-	chr4_2:15632697-15632716	None:intergenic	50.0%
	TGGCGCCATCCTTCACAAAA+TGG	-	chr4_2:15633050-15633069	None:intergenic	50.0%
!	TTTTGTGAAGGATGGCGCCA+TGG	+	chr4_2:15633050-15633069	Msa0591400:three_prime_UTR	50.0%
	GCCTATTGCTTGGTTGGCAC+TGG	+	chr4_2:15631965-15631984	Msa0591400:CDS	55.0%
!!	GTTGGTGGCTGTTCTGGTGT+TGG	+	chr4_2:15632157-15632176	Msa0591400:intron	55.0%
	CCCCATACAACCACACTCGA+AGG	+	chr4_2:15632567-15632586	Msa0591400:CDS	55.0%
	CTGTTATGGCGTTGCCATGG+AGG	+	chr4_2:15632991-15633010	Msa0591400:CDS	55.0%
	GCGTTGCCATGGAGGATTGT+GGG	+	chr4_2:15632999-15633018	Msa0591400:CDS	55.0%
	CGTTGCCATGGAGGATTGTG+GGG	+	chr4_2:15633000-15633019	Msa0591400:CDS	55.0%
	GTTGCCATGGAGGATTGTGG+GGG	+	chr4_2:15633001-15633020	Msa0591400:CDS	55.0%
	GATGGCGCCATGGTGCTTTA+TGG	+	chr4_2:15633060-15633079	Msa0591400:three_prime_UTR	55.0%
	GCCTATTGCTTGGTTGGCAC+TGG	+	chr4_2:15631965-15631984	Msa0591400:CDS	55.0%
!!	GTTGGTGGCTGTTCTGGTGT+TGG	+	chr4_2:15632157-15632176	Msa0591400:intron	55.0%
	CCCCATACAACCACACTCGA+AGG	+	chr4_2:15632567-15632586	Msa0591400:CDS	55.0%
	CTGTTATGGCGTTGCCATGG+AGG	+	chr4_2:15632991-15633010	Msa0591400:CDS	55.0%
	GCGTTGCCATGGAGGATTGT+GGG	+	chr4_2:15632999-15633018	Msa0591400:CDS	55.0%
	CGTTGCCATGGAGGATTGTG+GGG	+	chr4_2:15633000-15633019	Msa0591400:CDS	55.0%
	GTTGCCATGGAGGATTGTGG+GGG	+	chr4_2:15633001-15633020	Msa0591400:CDS	55.0%
	GATGGCGCCATGGTGCTTTA+TGG	+	chr4_2:15633060-15633079	Msa0591400:three_prime_UTR	55.0%
!!	ACCTCCGTTGGTGGCTGTTC+TGG	+	chr4_2:15632151-15632170	Msa0591400:intron	60.0%
	ACCAGAACAGCCACCAACGG+AGG	-	chr4_2:15632155-15632174	None:intergenic	60.0%
	GGCGTTGCCATGGAGGATTG+TGG	+	chr4_2:15632998-15633017	Msa0591400:CDS	60.0%
	TCAGCCCCCACAATCCTCCA+TGG	-	chr4_2:15633008-15633027	None:intergenic	60.0%
	GGCGCCATGGTGCTTTATGG+TGG	+	chr4_2:15633063-15633082	Msa0591400:three_prime_UTR	60.0%
!!	ACCTCCGTTGGTGGCTGTTC+TGG	+	chr4_2:15632151-15632170	Msa0591400:intron	60.0%
	ACCAGAACAGCCACCAACGG+AGG	-	chr4_2:15632155-15632174	None:intergenic	60.0%
	GGCGTTGCCATGGAGGATTG+TGG	+	chr4_2:15632998-15633017	Msa0591400:CDS	60.0%
	TCAGCCCCCACAATCCTCCA+TGG	-	chr4_2:15633008-15633027	None:intergenic	60.0%
	GGCGCCATGGTGCTTTATGG+TGG	+	chr4_2:15633063-15633082	Msa0591400:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	15631881	15633371	15631881	ID=Msa0591400;Name=Msa0591400
chr4_2	mRNA	15631881	15633371	15631881	ID=Msa0591400-mRNA-1;Parent=Msa0591400;Name=Msa0591400-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0.66\|0.5\|1\|0.66\|0.75\|4\|344\|91
chr4_2	exon	15631881	15632008	15631881	ID=Msa0591400-mRNA-1:exon:4466;Parent=Msa0591400-mRNA-1
chr4_2	exon	15632108	15632160	15632108	ID=Msa0591400-mRNA-1:exon:4467;Parent=Msa0591400-mRNA-1
chr4_2	exon	15632544	15632588	15632544	ID=Msa0591400-mRNA-1:exon:4468;Parent=Msa0591400-mRNA-1
chr4_2	exon	15632978	15633371	15632978	ID=Msa0591400-mRNA-1:exon:4469;Parent=Msa0591400-mRNA-1
chr4_2	CDS	15631881	15632008	15631881	ID=Msa0591400-mRNA-1:cds;Parent=Msa0591400-mRNA-1
chr4_2	CDS	15632108	15632160	15632108	ID=Msa0591400-mRNA-1:cds;Parent=Msa0591400-mRNA-1
chr4_2	CDS	15632544	15632588	15632544	ID=Msa0591400-mRNA-1:cds;Parent=Msa0591400-mRNA-1
chr4_2	CDS	15632978	15633027	15632978	ID=Msa0591400-mRNA-1:cds;Parent=Msa0591400-mRNA-1
chr4_2	three_prime_UTR	15633028	15633371	15633028	ID=Msa0591400-mRNA-1:three_prime_utr;Parent=Msa0591400-mRNA-1

Gene Sequence	>Msa0591400 ATGAACACACAATGGTATATGTCTCCTGTAGGACTTATGTTTGACGACAGCACTGAAATTGGATTTTATTGTAAACTCACCAATGCCTATTGCTTGGTTGGCACTGGAGGTTCCAATAACTTCTACAGTGCAATTGAGGCTGAACTAGAAGATGCGATCCCTATCATCAAAACCTCCGTTGGAAACAAAAATGGGCTTCTCTTGCCCCATACAACCACACTCGAAGTGCTGTCAAATATCTGTTATGGCGTTGCCATGGAGGATTGTGGGGGCTGA
Protein sequence	>Msa0591400 MNTQWYMSPVGLMFDDSTEIGFYCKLTNAYCLVGTGGSNNFYSAIEAELEDAIPIIKTSVGNKNGLLLPHTTTLEVLSNICYGVAMEDCGG*