AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0592030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592030 MtrunA17_Chr4g0010061 97.959 147 3 0 39 185 2 148 2.52e-105 298
Msa0592030 MtrunA17_Chr3g0127451 64.394 132 47 0 51 182 1 132 1.44e-61 187
Msa0592030 MtrunA17_Chr8g0346311 50.725 138 65 2 51 185 1 138 4.07e-49 155
Msa0592030 MtrunA17_Chr7g0276081 53.030 132 60 1 56 185 7 138 1.74e-39 131
Msa0592030 MtrunA17_Chr5g0419651 51.948 77 37 0 58 134 8 84 2.86e-29 105
Msa0592030 MtrunA17_Chr3g0134591 40.876 137 70 3 55 181 4 139 2.40e-28 103
Msa0592030 MtrunA17_Chr6g0486891 43.421 76 42 1 56 131 44 118 3.19e-19 79.0
Msa0592030 MtrunA17_Chr5g0424491 31.624 117 78 2 51 167 513 627 1.94e-16 76.6
Msa0592030 MtrunA17_Chr3g0115151 37.333 75 46 1 56 130 462 535 3.91e-16 75.5
Msa0592030 MtrunA17_Chr3g0115131 30.392 102 70 1 29 130 490 590 3.12e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0592030 AT2G32720.1 75.188 133 33 0 51 183 1 133 3.01e-75 222
Msa0592030 AT5G48810.1 63.704 135 47 1 51 183 1 135 6.98e-63 191
Msa0592030 AT5G53560.1 62.121 132 50 0 51 182 1 132 3.94e-62 189
Msa0592030 AT2G32720.2 66.165 133 45 0 51 183 1 133 2.72e-60 184
Msa0592030 AT2G46650.1 47.541 122 62 1 62 181 9 130 8.72e-33 114
Msa0592030 AT1G26340.1 37.037 135 73 3 56 183 6 135 8.36e-30 107
Msa0592030 AT1G37130.1 36.667 90 56 1 41 130 528 616 2.57e-18 82.4
Msa0592030 AT1G60660.1 40.000 75 44 1 56 130 47 120 1.96e-17 74.7
Msa0592030 AT1G77760.1 31.731 104 66 2 45 148 535 633 2.10e-16 76.6

Find 58 sgRNAs with CRISPR-Local

Find 261 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAATTGATATATCAAAAT+TGG 0.243550 4_2:-17101513 None:intergenic
TCTAGTTCCCTTGTTTATCT+TGG 0.247089 4_2:+17101170 Msa0592030:CDS
TGCTGCAAATGATCGGTTTA+TGG 0.264365 4_2:+17101542 Msa0592030:three_prime_UTR
TTCATCAACCATCCCTTCTA+AGG 0.292140 4_2:+17101071 Msa0592030:CDS
TAAACAAGGGAACTAGAAAT+TGG 0.327562 4_2:-17101164 None:intergenic
CTAGTTCCCTTGTTTATCTT+GGG 0.328366 4_2:+17101171 Msa0592030:CDS
ATTTCTAGAAGTTGAGAGTA+TGG 0.328710 4_2:+17099230 None:intergenic
TTATGATGTGACAAAGTTCT+TGG 0.338674 4_2:+17100803 Msa0592030:CDS
CCATAAAACTGTTCCGGTTC+CGG 0.345429 4_2:-17101440 None:intergenic
TTTAAATCATTGTGTTGATT+TGG 0.351861 4_2:-17101220 None:intergenic
AATAACAAGCCAGCAGTCTT+TGG 0.354976 4_2:-17099459 None:intergenic
CAAAGAATTGTGGTTGGTAT+AGG 0.368208 4_2:-17099303 None:intergenic
CATTCATTTGCTACCGGAAC+CGG 0.374004 4_2:+17101427 Msa0592030:three_prime_UTR
TGATTATAGTGAGGTTGTTT+CGG 0.378211 4_2:-17101108 None:intergenic
GGAGTGTAATCAACCTTAGA+AGG 0.397893 4_2:-17101084 None:intergenic
AAAACTCCATAAAACTGTTC+CGG 0.398008 4_2:-17101446 None:intergenic
AAACTATGGTATCTCAACTA+TGG 0.402447 4_2:-17101568 None:intergenic
ATTGTGGTTGGTATAGGTAA+TGG 0.406295 4_2:-17099297 None:intergenic
TTGTGGTTGGTATAGGTAAT+GGG 0.412176 4_2:-17099296 None:intergenic
CTCTGTCATTCATTTGCTAC+CGG 0.416188 4_2:+17101421 Msa0592030:three_prime_UTR
AACAGAAAATTACAAGGAAA+AGG 0.427313 4_2:-17101380 None:intergenic
GTATGGTATGTTATTATTGT+TGG 0.433443 4_2:+17099247 Msa0592030:exon
GAAACAACCTCACTATAATC+AGG 0.469523 4_2:+17101110 Msa0592030:CDS
GCTGCAAATGATCGGTTTAT+GGG 0.470730 4_2:+17101543 Msa0592030:three_prime_UTR
TTCAAATGTTCAAACTAATG+AGG 0.473057 4_2:+17101320 Msa0592030:three_prime_UTR
TCCCTTGTTTATCTTGGGTG+TGG 0.477784 4_2:+17101176 Msa0592030:CDS
GGTAATGGGTTGGTGAAGGT+TGG 0.478047 4_2:-17099282 None:intergenic
ATTGTTGAGTGTCGAAAGAC+AGG 0.483965 4_2:+17101286 Msa0592030:three_prime_UTR
TTTATCTTGGGTGTGGCAGT+TGG 0.495453 4_2:+17101183 Msa0592030:CDS
CACAAGTGCTTATACAATGA+TGG 0.506012 4_2:+17101023 Msa0592030:CDS
GAAGTTCTAATTTCTTCCAC+AGG 0.521676 4_2:+17100846 Msa0592030:CDS
TCAAACTAATGAGGTTTGCT+AGG 0.523619 4_2:+17101329 Msa0592030:three_prime_UTR
ATAACAGTGCCAAAGACTGC+TGG 0.526724 4_2:+17099450 Msa0592030:CDS
TGATGTGACAAAGTTCTTGG+AGG 0.531325 4_2:+17100806 Msa0592030:CDS
TATAGGTAATGGGTTGGTGA+AGG 0.531491 4_2:-17099286 None:intergenic
AACAATGGGTGAAGAGCGTA+AGG 0.547987 4_2:+17099400 Msa0592030:CDS
AACACAATGATTTAAACAGC+AGG 0.555728 4_2:+17101227 Msa0592030:exon
AGAGCGTAAGGTGTTCACTT+TGG 0.558742 4_2:+17099412 Msa0592030:CDS
CTGGCTTGTTATTCATAACA+AGG 0.559254 4_2:+17099469 Msa0592030:CDS
GGTTGGTATAGGTAATGGGT+TGG 0.563883 4_2:-17099292 None:intergenic
TTATAGTGAGGTTGTTTCGG+TGG 0.569690 4_2:-17101105 None:intergenic
GTAATCAACCTTAGAAGGGA+TGG 0.570185 4_2:-17101079 None:intergenic
AGTACTGATCAATCAACAAT+GGG 0.587252 4_2:+17099386 Msa0592030:CDS
ACAAGGGAACTAGAAATTGG+AGG 0.592277 4_2:-17101161 None:intergenic
AGATTTAACAGAAAATTACA+AGG 0.596906 4_2:-17101386 None:intergenic
GAATAGCAAAGAATTGTGGT+TGG 0.609280 4_2:-17099309 None:intergenic
ATTAGAACTTCATCTCCACC+AGG 0.612765 4_2:-17100834 None:intergenic
GGTGTGATGCTGCAAATGAT+CGG 0.622927 4_2:+17101535 Msa0592030:three_prime_UTR
GAGTGTAATCAACCTTAGAA+GGG 0.626121 4_2:-17101083 None:intergenic
TTCTTGGAGGACCACCCTGG+TGG 0.634311 4_2:+17100819 Msa0592030:CDS
GAATGAATAGCAAAGAATTG+TGG 0.638555 4_2:-17099313 None:intergenic
AAGTTCTTGGAGGACCACCC+TGG 0.645731 4_2:+17100816 Msa0592030:CDS
TGCCACACCCAAGATAAACA+AGG 0.648597 4_2:-17101178 None:intergenic
GAGTACTGATCAATCAACAA+TGG 0.648670 4_2:+17099385 Msa0592030:CDS
GTCTTGTCCTGATTATAGTG+AGG 0.656778 4_2:-17101117 None:intergenic
GCCACACCCAAGATAAACAA+GGG 0.664866 4_2:-17101177 None:intergenic
GAACTTCATCTCCACCAGGG+TGG 0.732823 4_2:-17100830 None:intergenic
TTAGAACTTCATCTCCACCA+GGG 0.757569 4_2:-17100833 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTGTTTGTAATTTGTT+TGG + chr4_2:17099517-17099536 Msa0592030:intron 15.0%
!! ATTTAAATCTATGTAAGAAT+TGG + chr4_2:17099711-17099730 Msa0592030:intron 15.0%
!!! AAATGTTACATTTTGTATAA+AGG + chr4_2:17099750-17099769 Msa0592030:intron 15.0%
!!! AATGTTACATTTTGTATAAA+GGG + chr4_2:17099751-17099770 Msa0592030:intron 15.0%
!!! AAGTTTCTTTTTATCTTTTA+GGG + chr4_2:17100712-17100731 Msa0592030:intron 15.0%
!!! GTTTTAACAGTATAAAATTT+TGG + chr4_2:17101464-17101483 Msa0592030:three_prime_UTR 15.0%
!! CATAATTGATATATCAAAAT+TGG - chr4_2:17101516-17101535 None:intergenic 15.0%
!!! CAATTTTGATATATCAATTA+TGG + chr4_2:17101514-17101533 Msa0592030:three_prime_UTR 15.0%
!!! TTTTTTGTTTGTAATTTGTT+TGG + chr4_2:17099517-17099536 Msa0592030:intron 15.0%
!! ATTTAAATCTATGTAAGAAT+TGG + chr4_2:17099711-17099730 Msa0592030:intron 15.0%
!!! AAATGTTACATTTTGTATAA+AGG + chr4_2:17099750-17099769 Msa0592030:intron 15.0%
!!! AATGTTACATTTTGTATAAA+GGG + chr4_2:17099751-17099770 Msa0592030:intron 15.0%
!!! AAGTTTCTTTTTATCTTTTA+GGG + chr4_2:17100712-17100731 Msa0592030:intron 15.0%
!!! GTTTTAACAGTATAAAATTT+TGG + chr4_2:17101464-17101483 Msa0592030:three_prime_UTR 15.0%
!! CATAATTGATATATCAAAAT+TGG - chr4_2:17101516-17101535 None:intergenic 15.0%
!!! CAATTTTGATATATCAATTA+TGG + chr4_2:17101514-17101533 Msa0592030:three_prime_UTR 15.0%
!! TTTGTTTGTTTGTGTTAAAA+TGG + chr4_2:17099570-17099589 Msa0592030:intron 20.0%
!!! TTTGATTGTGTTTTTTTTAC+TGG + chr4_2:17099801-17099820 Msa0592030:intron 20.0%
!!! TTTAGATTGTGTTTTTATTC+TGG + chr4_2:17099878-17099897 Msa0592030:intron 20.0%
!! AACAAATTGAAATTTGCATA+TGG - chr4_2:17100172-17100191 None:intergenic 20.0%
!! AGAATACAATTCAAAATAGT+AGG - chr4_2:17100342-17100361 None:intergenic 20.0%
!!! ACTTATTTGAGTTTATCTAT+GGG + chr4_2:17100432-17100451 Msa0592030:intron 20.0%
!! ACTTATAAACATGTCATAAT+AGG - chr4_2:17100488-17100507 None:intergenic 20.0%
!!! GAAGTTTCTTTTTATCTTTT+AGG + chr4_2:17100711-17100730 Msa0592030:intron 20.0%
!!! TTTAAATCATTGTGTTGATT+TGG - chr4_2:17101223-17101242 None:intergenic 20.0%
!! AGATTTAACAGAAAATTACA+AGG - chr4_2:17101389-17101408 None:intergenic 20.0%
!! TTTGTTTGTTTGTGTTAAAA+TGG + chr4_2:17099570-17099589 Msa0592030:intron 20.0%
!!! TTTGATTGTGTTTTTTTTAC+TGG + chr4_2:17099801-17099820 Msa0592030:intron 20.0%
!!! TTTAGATTGTGTTTTTATTC+TGG + chr4_2:17099878-17099897 Msa0592030:intron 20.0%
!! AACAAATTGAAATTTGCATA+TGG - chr4_2:17100172-17100191 None:intergenic 20.0%
!! AGAATACAATTCAAAATAGT+AGG - chr4_2:17100342-17100361 None:intergenic 20.0%
!!! ACTTATTTGAGTTTATCTAT+GGG + chr4_2:17100432-17100451 Msa0592030:intron 20.0%
!! ACTTATAAACATGTCATAAT+AGG - chr4_2:17100488-17100507 None:intergenic 20.0%
!!! GAAGTTTCTTTTTATCTTTT+AGG + chr4_2:17100711-17100730 Msa0592030:intron 20.0%
!!! TTTAAATCATTGTGTTGATT+TGG - chr4_2:17101223-17101242 None:intergenic 20.0%
!! AGATTTAACAGAAAATTACA+AGG - chr4_2:17101389-17101408 None:intergenic 20.0%
! GTATGGTATGTTATTATTGT+TGG + chr4_2:17099247-17099266 Msa0592030:exon 25.0%
! AGTATTTGAAAAAAAGAGGT+GGG - chr4_2:17099353-17099372 None:intergenic 25.0%
!!! TGAGAGTATTTGAAAAAAAG+AGG - chr4_2:17099357-17099376 None:intergenic 25.0%
!! TTGTTTGAAATTCTAGTGTT+TGG + chr4_2:17099543-17099562 Msa0592030:intron 25.0%
!!! ATGTGTGTTATTTTGTCAAA+TGG + chr4_2:17099913-17099932 Msa0592030:intron 25.0%
! TTGAACAAATCGAAATTGTT+TGG + chr4_2:17099947-17099966 Msa0592030:intron 25.0%
!!! ATAGCGGAATTTTAACAAAA+TGG + chr4_2:17099993-17100012 Msa0592030:intron 25.0%
! TTTGTATTGAAAGCTTAAGT+TGG + chr4_2:17100361-17100380 Msa0592030:intron 25.0%
! AGAAAAGTAGATGTTGAATT+AGG + chr4_2:17100395-17100414 Msa0592030:intron 25.0%
! GAAAAGTAGATGTTGAATTA+GGG + chr4_2:17100396-17100415 Msa0592030:intron 25.0%
! TCAAATAAGTCAATCCAAAT+AGG - chr4_2:17100422-17100441 None:intergenic 25.0%
!! GACTTATTTGAGTTTATCTA+TGG + chr4_2:17100431-17100450 Msa0592030:intron 25.0%
!!! ATCTTTTTCAGAGAAAATGT+AGG - chr4_2:17100875-17100894 None:intergenic 25.0%
!!! TCAACTGATTTTGATGATAT+CGG + chr4_2:17100994-17101013 Msa0592030:CDS 25.0%
! TTCAAATGTTCAAACTAATG+AGG + chr4_2:17101320-17101339 Msa0592030:three_prime_UTR 25.0%
! AACAGAAAATTACAAGGAAA+AGG - chr4_2:17101383-17101402 None:intergenic 25.0%
! GTATGGTATGTTATTATTGT+TGG + chr4_2:17099247-17099266 Msa0592030:exon 25.0%
! AGTATTTGAAAAAAAGAGGT+GGG - chr4_2:17099353-17099372 None:intergenic 25.0%
!!! TGAGAGTATTTGAAAAAAAG+AGG - chr4_2:17099357-17099376 None:intergenic 25.0%
!! TTGTTTGAAATTCTAGTGTT+TGG + chr4_2:17099543-17099562 Msa0592030:intron 25.0%
!!! ATGTGTGTTATTTTGTCAAA+TGG + chr4_2:17099913-17099932 Msa0592030:intron 25.0%
! TTGAACAAATCGAAATTGTT+TGG + chr4_2:17099947-17099966 Msa0592030:intron 25.0%
!!! ATAGCGGAATTTTAACAAAA+TGG + chr4_2:17099993-17100012 Msa0592030:intron 25.0%
! TTTGTATTGAAAGCTTAAGT+TGG + chr4_2:17100361-17100380 Msa0592030:intron 25.0%
! AGAAAAGTAGATGTTGAATT+AGG + chr4_2:17100395-17100414 Msa0592030:intron 25.0%
! GAAAAGTAGATGTTGAATTA+GGG + chr4_2:17100396-17100415 Msa0592030:intron 25.0%
! TCAAATAAGTCAATCCAAAT+AGG - chr4_2:17100422-17100441 None:intergenic 25.0%
!! GACTTATTTGAGTTTATCTA+TGG + chr4_2:17100431-17100450 Msa0592030:intron 25.0%
!!! ATCTTTTTCAGAGAAAATGT+AGG - chr4_2:17100875-17100894 None:intergenic 25.0%
!!! TCAACTGATTTTGATGATAT+CGG + chr4_2:17100994-17101013 Msa0592030:CDS 25.0%
! TTCAAATGTTCAAACTAATG+AGG + chr4_2:17101320-17101339 Msa0592030:three_prime_UTR 25.0%
! AACAGAAAATTACAAGGAAA+AGG - chr4_2:17101383-17101402 None:intergenic 25.0%
GAATGAATAGCAAAGAATTG+TGG - chr4_2:17099316-17099335 None:intergenic 30.0%
GAGTATTTGAAAAAAAGAGG+TGG - chr4_2:17099354-17099373 None:intergenic 30.0%
!! AGTACTGATCAATCAACAAT+GGG + chr4_2:17099386-17099405 Msa0592030:CDS 30.0%
AATGGAATTCACAAGTGATA+TGG + chr4_2:17099588-17099607 Msa0592030:intron 30.0%
!!! GTGTTTTTTTTACTGGTATG+CGG + chr4_2:17099808-17099827 Msa0592030:intron 30.0%
AAACACAATCTAAATGCAAG+TGG - chr4_2:17099872-17099891 None:intergenic 30.0%
! TATTTTGTCAAATGGCTTAG+CGG + chr4_2:17099921-17099940 Msa0592030:intron 30.0%
!!! ATGGTATTTTTTGCAATCTG+TGG + chr4_2:17100012-17100031 Msa0592030:intron 30.0%
!! ACAACTATGAAAGAGTACTT+TGG - chr4_2:17100133-17100152 None:intergenic 30.0%
ACGTGTTAAGTTTCATATTC+TGG + chr4_2:17100251-17100270 Msa0592030:intron 30.0%
!! ATATTCTGGAATGAAGATCA+TGG + chr4_2:17100265-17100284 Msa0592030:intron 30.0%
! CAAAATCAACTTTTCCGTAT+TGG - chr4_2:17100293-17100312 None:intergenic 30.0%
!! AAAGCTTAAGTTGGTAAACA+AGG + chr4_2:17100370-17100389 Msa0592030:intron 30.0%
AAACACTTATGAGACTGTTT+AGG + chr4_2:17100458-17100477 Msa0592030:intron 30.0%
TAAGATCTCAGAATAGCTTA+TGG + chr4_2:17100527-17100546 Msa0592030:intron 30.0%
AATAAGTTGTTCATCCAAAC+AGG + chr4_2:17100643-17100662 Msa0592030:intron 30.0%
ATAAGTTGTTCATCCAAACA+GGG + chr4_2:17100644-17100663 Msa0592030:intron 30.0%
! GATTTGAAATTATCCTGTGT+TGG + chr4_2:17100736-17100755 Msa0592030:intron 30.0%
AATAGACATGGTAAGGTAAA+GGG - chr4_2:17100772-17100791 None:intergenic 30.0%
AAATAGACATGGTAAGGTAA+AGG - chr4_2:17100773-17100792 None:intergenic 30.0%
! TTACCTTACCATGTCTATTT+TGG + chr4_2:17100773-17100792 Msa0592030:intron 30.0%
TTATGATGTGACAAAGTTCT+TGG + chr4_2:17100803-17100822 Msa0592030:CDS 30.0%
!! GTGTTGTTTCGTTTATCAAA+AGG - chr4_2:17100953-17100972 None:intergenic 30.0%
!! ATGATGGAAGAGTTTTATGT+TGG + chr4_2:17101039-17101058 Msa0592030:CDS 30.0%
TGATTATAGTGAGGTTGTTT+CGG - chr4_2:17101111-17101130 None:intergenic 30.0%
TAAACAAGGGAACTAGAAAT+TGG - chr4_2:17101167-17101186 None:intergenic 30.0%
AACACAATGATTTAAACAGC+AGG + chr4_2:17101227-17101246 Msa0592030:exon 30.0%
AAAACTCCATAAAACTGTTC+CGG - chr4_2:17101449-17101468 None:intergenic 30.0%
GAATGAATAGCAAAGAATTG+TGG - chr4_2:17099316-17099335 None:intergenic 30.0%
GAGTATTTGAAAAAAAGAGG+TGG - chr4_2:17099354-17099373 None:intergenic 30.0%
!! AGTACTGATCAATCAACAAT+GGG + chr4_2:17099386-17099405 Msa0592030:CDS 30.0%
AATGGAATTCACAAGTGATA+TGG + chr4_2:17099588-17099607 Msa0592030:intron 30.0%
!!! GTGTTTTTTTTACTGGTATG+CGG + chr4_2:17099808-17099827 Msa0592030:intron 30.0%
AAACACAATCTAAATGCAAG+TGG - chr4_2:17099872-17099891 None:intergenic 30.0%
! TATTTTGTCAAATGGCTTAG+CGG + chr4_2:17099921-17099940 Msa0592030:intron 30.0%
!!! ATGGTATTTTTTGCAATCTG+TGG + chr4_2:17100012-17100031 Msa0592030:intron 30.0%
!! ACAACTATGAAAGAGTACTT+TGG - chr4_2:17100133-17100152 None:intergenic 30.0%
ACGTGTTAAGTTTCATATTC+TGG + chr4_2:17100251-17100270 Msa0592030:intron 30.0%
!! ATATTCTGGAATGAAGATCA+TGG + chr4_2:17100265-17100284 Msa0592030:intron 30.0%
! CAAAATCAACTTTTCCGTAT+TGG - chr4_2:17100293-17100312 None:intergenic 30.0%
!! AAAGCTTAAGTTGGTAAACA+AGG + chr4_2:17100370-17100389 Msa0592030:intron 30.0%
AAACACTTATGAGACTGTTT+AGG + chr4_2:17100458-17100477 Msa0592030:intron 30.0%
TAAGATCTCAGAATAGCTTA+TGG + chr4_2:17100527-17100546 Msa0592030:intron 30.0%
AATAAGTTGTTCATCCAAAC+AGG + chr4_2:17100643-17100662 Msa0592030:intron 30.0%
ATAAGTTGTTCATCCAAACA+GGG + chr4_2:17100644-17100663 Msa0592030:intron 30.0%
! GATTTGAAATTATCCTGTGT+TGG + chr4_2:17100736-17100755 Msa0592030:intron 30.0%
AATAGACATGGTAAGGTAAA+GGG - chr4_2:17100772-17100791 None:intergenic 30.0%
AAATAGACATGGTAAGGTAA+AGG - chr4_2:17100773-17100792 None:intergenic 30.0%
! TTACCTTACCATGTCTATTT+TGG + chr4_2:17100773-17100792 Msa0592030:intron 30.0%
TTATGATGTGACAAAGTTCT+TGG + chr4_2:17100803-17100822 Msa0592030:CDS 30.0%
!! GTGTTGTTTCGTTTATCAAA+AGG - chr4_2:17100953-17100972 None:intergenic 30.0%
!! ATGATGGAAGAGTTTTATGT+TGG + chr4_2:17101039-17101058 Msa0592030:CDS 30.0%
TGATTATAGTGAGGTTGTTT+CGG - chr4_2:17101111-17101130 None:intergenic 30.0%
TAAACAAGGGAACTAGAAAT+TGG - chr4_2:17101167-17101186 None:intergenic 30.0%
AACACAATGATTTAAACAGC+AGG + chr4_2:17101227-17101246 Msa0592030:exon 30.0%
AAAACTCCATAAAACTGTTC+CGG - chr4_2:17101449-17101468 None:intergenic 30.0%
! TTGTGGTTGGTATAGGTAAT+GGG - chr4_2:17099299-17099318 None:intergenic 35.0%
! ATTGTGGTTGGTATAGGTAA+TGG - chr4_2:17099300-17099319 None:intergenic 35.0%
! CAAAGAATTGTGGTTGGTAT+AGG - chr4_2:17099306-17099325 None:intergenic 35.0%
GAATAGCAAAGAATTGTGGT+TGG - chr4_2:17099312-17099331 None:intergenic 35.0%
ATTTGAAAAAAAGAGGTGGG+TGG - chr4_2:17099350-17099369 None:intergenic 35.0%
!! GAGTACTGATCAATCAACAA+TGG + chr4_2:17099385-17099404 Msa0592030:CDS 35.0%
!! CTGGCTTGTTATTCATAACA+AGG + chr4_2:17099469-17099488 Msa0592030:CDS 35.0%
CTATTTAGCACATAGCATAG+CGG + chr4_2:17099977-17099996 Msa0592030:intron 35.0%
TGAAGATCATGGTTCCAATA+CGG + chr4_2:17100276-17100295 Msa0592030:intron 35.0%
ATGTTGAATTAGGGCCTATT+TGG + chr4_2:17100405-17100424 Msa0592030:intron 35.0%
GTTCCAAAATAGACATGGTA+AGG - chr4_2:17100779-17100798 None:intergenic 35.0%
!!! TACCATGTCTATTTTGGAAC+AGG + chr4_2:17100779-17100798 Msa0592030:intron 35.0%
AACCTGTTCCAAAATAGACA+TGG - chr4_2:17100784-17100803 None:intergenic 35.0%
! GAAGTTCTAATTTCTTCCAC+AGG + chr4_2:17100846-17100865 Msa0592030:CDS 35.0%
CACAAGTGCTTATACAATGA+TGG + chr4_2:17101023-17101042 Msa0592030:CDS 35.0%
GAGTGTAATCAACCTTAGAA+GGG - chr4_2:17101086-17101105 None:intergenic 35.0%
GAAACAACCTCACTATAATC+AGG + chr4_2:17101110-17101129 Msa0592030:CDS 35.0%
! TCTAGTTCCCTTGTTTATCT+TGG + chr4_2:17101170-17101189 Msa0592030:CDS 35.0%
! CTAGTTCCCTTGTTTATCTT+GGG + chr4_2:17101171-17101190 Msa0592030:CDS 35.0%
! TCAAACTAATGAGGTTTGCT+AGG + chr4_2:17101329-17101348 Msa0592030:three_prime_UTR 35.0%
! TTGTGGTTGGTATAGGTAAT+GGG - chr4_2:17099299-17099318 None:intergenic 35.0%
! ATTGTGGTTGGTATAGGTAA+TGG - chr4_2:17099300-17099319 None:intergenic 35.0%
! CAAAGAATTGTGGTTGGTAT+AGG - chr4_2:17099306-17099325 None:intergenic 35.0%
GAATAGCAAAGAATTGTGGT+TGG - chr4_2:17099312-17099331 None:intergenic 35.0%
ATTTGAAAAAAAGAGGTGGG+TGG - chr4_2:17099350-17099369 None:intergenic 35.0%
!! GAGTACTGATCAATCAACAA+TGG + chr4_2:17099385-17099404 Msa0592030:CDS 35.0%
!! CTGGCTTGTTATTCATAACA+AGG + chr4_2:17099469-17099488 Msa0592030:CDS 35.0%
CTATTTAGCACATAGCATAG+CGG + chr4_2:17099977-17099996 Msa0592030:intron 35.0%
TGAAGATCATGGTTCCAATA+CGG + chr4_2:17100276-17100295 Msa0592030:intron 35.0%
ATGTTGAATTAGGGCCTATT+TGG + chr4_2:17100405-17100424 Msa0592030:intron 35.0%
GTTCCAAAATAGACATGGTA+AGG - chr4_2:17100779-17100798 None:intergenic 35.0%
!!! TACCATGTCTATTTTGGAAC+AGG + chr4_2:17100779-17100798 Msa0592030:intron 35.0%
AACCTGTTCCAAAATAGACA+TGG - chr4_2:17100784-17100803 None:intergenic 35.0%
! GAAGTTCTAATTTCTTCCAC+AGG + chr4_2:17100846-17100865 Msa0592030:CDS 35.0%
CACAAGTGCTTATACAATGA+TGG + chr4_2:17101023-17101042 Msa0592030:CDS 35.0%
GAGTGTAATCAACCTTAGAA+GGG - chr4_2:17101086-17101105 None:intergenic 35.0%
GAAACAACCTCACTATAATC+AGG + chr4_2:17101110-17101129 Msa0592030:CDS 35.0%
! TCTAGTTCCCTTGTTTATCT+TGG + chr4_2:17101170-17101189 Msa0592030:CDS 35.0%
! CTAGTTCCCTTGTTTATCTT+GGG + chr4_2:17101171-17101190 Msa0592030:CDS 35.0%
! TCAAACTAATGAGGTTTGCT+AGG + chr4_2:17101329-17101348 Msa0592030:three_prime_UTR 35.0%
!! TATAGGTAATGGGTTGGTGA+AGG - chr4_2:17099289-17099308 None:intergenic 40.0%
AATAACAAGCCAGCAGTCTT+TGG - chr4_2:17099462-17099481 None:intergenic 40.0%
ACAAAGACCGACTAATCTGA+GGG + chr4_2:17099832-17099851 Msa0592030:intron 40.0%
!! TCTAAATGCAAGTGGACTGT+TGG - chr4_2:17099864-17099883 None:intergenic 40.0%
TCTTCTACTAAAGCCCTGTT+TGG - chr4_2:17100660-17100679 None:intergenic 40.0%
TGATGTGACAAAGTTCTTGG+AGG + chr4_2:17100806-17100825 Msa0592030:CDS 40.0%
TTAGAACTTCATCTCCACCA+GGG - chr4_2:17100836-17100855 None:intergenic 40.0%
ATTAGAACTTCATCTCCACC+AGG - chr4_2:17100837-17100856 None:intergenic 40.0%
GAGAAAATGTAGGATACCTG+TGG - chr4_2:17100865-17100884 None:intergenic 40.0%
!! ATCTCACTTCTGAGATCATG+AGG - chr4_2:17100917-17100936 None:intergenic 40.0%
ACGAAACAACACATGTTTGC+AGG + chr4_2:17100961-17100980 Msa0592030:intron 40.0%
CGAAACAACACATGTTTGCA+GGG + chr4_2:17100962-17100981 Msa0592030:intron 40.0%
TTCATCAACCATCCCTTCTA+AGG + chr4_2:17101071-17101090 Msa0592030:CDS 40.0%
GTAATCAACCTTAGAAGGGA+TGG - chr4_2:17101082-17101101 None:intergenic 40.0%
GGAGTGTAATCAACCTTAGA+AGG - chr4_2:17101087-17101106 None:intergenic 40.0%
! TTATAGTGAGGTTGTTTCGG+TGG - chr4_2:17101108-17101127 None:intergenic 40.0%
GTCTTGTCCTGATTATAGTG+AGG - chr4_2:17101120-17101139 None:intergenic 40.0%
ACAAGGGAACTAGAAATTGG+AGG - chr4_2:17101164-17101183 None:intergenic 40.0%
!! ATTGTTGAGTGTCGAAAGAC+AGG + chr4_2:17101286-17101305 Msa0592030:three_prime_UTR 40.0%
! CTCTGTCATTCATTTGCTAC+CGG + chr4_2:17101421-17101440 Msa0592030:three_prime_UTR 40.0%
! TGCTGCAAATGATCGGTTTA+TGG + chr4_2:17101542-17101561 Msa0592030:three_prime_UTR 40.0%
! GCTGCAAATGATCGGTTTAT+GGG + chr4_2:17101543-17101562 Msa0592030:three_prime_UTR 40.0%
GTTTATGGGAACCATAGTTG+AGG + chr4_2:17101557-17101576 Msa0592030:three_prime_UTR 40.0%
!! TATAGGTAATGGGTTGGTGA+AGG - chr4_2:17099289-17099308 None:intergenic 40.0%
AATAACAAGCCAGCAGTCTT+TGG - chr4_2:17099462-17099481 None:intergenic 40.0%
ACAAAGACCGACTAATCTGA+GGG + chr4_2:17099832-17099851 Msa0592030:intron 40.0%
!! TCTAAATGCAAGTGGACTGT+TGG - chr4_2:17099864-17099883 None:intergenic 40.0%
TCTTCTACTAAAGCCCTGTT+TGG - chr4_2:17100660-17100679 None:intergenic 40.0%
TGATGTGACAAAGTTCTTGG+AGG + chr4_2:17100806-17100825 Msa0592030:CDS 40.0%
TTAGAACTTCATCTCCACCA+GGG - chr4_2:17100836-17100855 None:intergenic 40.0%
ATTAGAACTTCATCTCCACC+AGG - chr4_2:17100837-17100856 None:intergenic 40.0%
GAGAAAATGTAGGATACCTG+TGG - chr4_2:17100865-17100884 None:intergenic 40.0%
!! ATCTCACTTCTGAGATCATG+AGG - chr4_2:17100917-17100936 None:intergenic 40.0%
ACGAAACAACACATGTTTGC+AGG + chr4_2:17100961-17100980 Msa0592030:intron 40.0%
CGAAACAACACATGTTTGCA+GGG + chr4_2:17100962-17100981 Msa0592030:intron 40.0%
TTCATCAACCATCCCTTCTA+AGG + chr4_2:17101071-17101090 Msa0592030:CDS 40.0%
GTAATCAACCTTAGAAGGGA+TGG - chr4_2:17101082-17101101 None:intergenic 40.0%
GGAGTGTAATCAACCTTAGA+AGG - chr4_2:17101087-17101106 None:intergenic 40.0%
! TTATAGTGAGGTTGTTTCGG+TGG - chr4_2:17101108-17101127 None:intergenic 40.0%
GTCTTGTCCTGATTATAGTG+AGG - chr4_2:17101120-17101139 None:intergenic 40.0%
ACAAGGGAACTAGAAATTGG+AGG - chr4_2:17101164-17101183 None:intergenic 40.0%
!! ATTGTTGAGTGTCGAAAGAC+AGG + chr4_2:17101286-17101305 Msa0592030:three_prime_UTR 40.0%
! CTCTGTCATTCATTTGCTAC+CGG + chr4_2:17101421-17101440 Msa0592030:three_prime_UTR 40.0%
! TGCTGCAAATGATCGGTTTA+TGG + chr4_2:17101542-17101561 Msa0592030:three_prime_UTR 40.0%
! GCTGCAAATGATCGGTTTAT+GGG + chr4_2:17101543-17101562 Msa0592030:three_prime_UTR 40.0%
!! GGTTGGTATAGGTAATGGGT+TGG - chr4_2:17099295-17099314 None:intergenic 45.0%
GAAAAAAAGAGGTGGGTGGT+TGG - chr4_2:17099346-17099365 None:intergenic 45.0%
! AACAATGGGTGAAGAGCGTA+AGG + chr4_2:17099400-17099419 Msa0592030:CDS 45.0%
! AGAGCGTAAGGTGTTCACTT+TGG + chr4_2:17099412-17099431 Msa0592030:CDS 45.0%
ATAACAGTGCCAAAGACTGC+TGG + chr4_2:17099450-17099469 Msa0592030:CDS 45.0%
GTTGTGACAACCTTAGTTGC+AGG + chr4_2:17099664-17099683 Msa0592030:intron 45.0%
GACAACCTTAGTTGCAGGAT+TGG + chr4_2:17099669-17099688 Msa0592030:intron 45.0%
CCAATCCAATCCTGCAACTA+AGG - chr4_2:17099677-17099696 None:intergenic 45.0%
CCTTAGTTGCAGGATTGGAT+TGG + chr4_2:17099674-17099693 Msa0592030:intron 45.0%
! GTTGCAGGATTGGATTGGAT+TGG + chr4_2:17099679-17099698 Msa0592030:intron 45.0%
CACAAAGACCGACTAATCTG+AGG + chr4_2:17099831-17099850 Msa0592030:intron 45.0%
! GATTGGTCCCTCAGATTAGT+CGG - chr4_2:17099842-17099861 None:intergenic 45.0%
!! ATGCAAGTGGACTGTTGGAT+TGG - chr4_2:17099859-17099878 None:intergenic 45.0%
! TCCCTTGTTTATCTTGGGTG+TGG + chr4_2:17101176-17101195 Msa0592030:CDS 45.0%
GCCACACCCAAGATAAACAA+GGG - chr4_2:17101180-17101199 None:intergenic 45.0%
TGCCACACCCAAGATAAACA+AGG - chr4_2:17101181-17101200 None:intergenic 45.0%
TTTATCTTGGGTGTGGCAGT+TGG + chr4_2:17101183-17101202 Msa0592030:CDS 45.0%
! CATTCATTTGCTACCGGAAC+CGG + chr4_2:17101427-17101446 Msa0592030:three_prime_UTR 45.0%
CCATAAAACTGTTCCGGTTC+CGG - chr4_2:17101443-17101462 None:intergenic 45.0%
GGTGTGATGCTGCAAATGAT+CGG + chr4_2:17101535-17101554 Msa0592030:three_prime_UTR 45.0%
!! GGTTGGTATAGGTAATGGGT+TGG - chr4_2:17099295-17099314 None:intergenic 45.0%
GAAAAAAAGAGGTGGGTGGT+TGG - chr4_2:17099346-17099365 None:intergenic 45.0%
! AACAATGGGTGAAGAGCGTA+AGG + chr4_2:17099400-17099419 Msa0592030:CDS 45.0%
! AGAGCGTAAGGTGTTCACTT+TGG + chr4_2:17099412-17099431 Msa0592030:CDS 45.0%
ATAACAGTGCCAAAGACTGC+TGG + chr4_2:17099450-17099469 Msa0592030:CDS 45.0%
GTTGTGACAACCTTAGTTGC+AGG + chr4_2:17099664-17099683 Msa0592030:intron 45.0%
GACAACCTTAGTTGCAGGAT+TGG + chr4_2:17099669-17099688 Msa0592030:intron 45.0%
CCAATCCAATCCTGCAACTA+AGG - chr4_2:17099677-17099696 None:intergenic 45.0%
CCTTAGTTGCAGGATTGGAT+TGG + chr4_2:17099674-17099693 Msa0592030:intron 45.0%
! GTTGCAGGATTGGATTGGAT+TGG + chr4_2:17099679-17099698 Msa0592030:intron 45.0%
CACAAAGACCGACTAATCTG+AGG + chr4_2:17099831-17099850 Msa0592030:intron 45.0%
! GATTGGTCCCTCAGATTAGT+CGG - chr4_2:17099842-17099861 None:intergenic 45.0%
!! ATGCAAGTGGACTGTTGGAT+TGG - chr4_2:17099859-17099878 None:intergenic 45.0%
! TCCCTTGTTTATCTTGGGTG+TGG + chr4_2:17101176-17101195 Msa0592030:CDS 45.0%
GCCACACCCAAGATAAACAA+GGG - chr4_2:17101180-17101199 None:intergenic 45.0%
TGCCACACCCAAGATAAACA+AGG - chr4_2:17101181-17101200 None:intergenic 45.0%
TTTATCTTGGGTGTGGCAGT+TGG + chr4_2:17101183-17101202 Msa0592030:CDS 45.0%
! CATTCATTTGCTACCGGAAC+CGG + chr4_2:17101427-17101446 Msa0592030:three_prime_UTR 45.0%
CCATAAAACTGTTCCGGTTC+CGG - chr4_2:17101443-17101462 None:intergenic 45.0%
GGTGTGATGCTGCAAATGAT+CGG + chr4_2:17101535-17101554 Msa0592030:three_prime_UTR 45.0%
!! GGTAATGGGTTGGTGAAGGT+TGG - chr4_2:17099285-17099304 None:intergenic 50.0%
! CCGGAACCGGAACAGTTTTA+TGG + chr4_2:17101440-17101459 Msa0592030:three_prime_UTR 50.0%
!! GGTAATGGGTTGGTGAAGGT+TGG - chr4_2:17099285-17099304 None:intergenic 50.0%
! CCGGAACCGGAACAGTTTTA+TGG + chr4_2:17101440-17101459 Msa0592030:three_prime_UTR 50.0%
GGGACAGAAGACACCAACAC+AGG - chr4_2:17100752-17100771 None:intergenic 55.0%
AAGTTCTTGGAGGACCACCC+TGG + chr4_2:17100816-17100835 Msa0592030:CDS 55.0%
GAACTTCATCTCCACCAGGG+TGG - chr4_2:17100833-17100852 None:intergenic 55.0%
GGGACAGAAGACACCAACAC+AGG - chr4_2:17100752-17100771 None:intergenic 55.0%
AAGTTCTTGGAGGACCACCC+TGG + chr4_2:17100816-17100835 Msa0592030:CDS 55.0%
GAACTTCATCTCCACCAGGG+TGG - chr4_2:17100833-17100852 None:intergenic 55.0%
TTCTTGGAGGACCACCCTGG+TGG + chr4_2:17100819-17100838 Msa0592030:CDS 60.0%
TTCTTGGAGGACCACCCTGG+TGG + chr4_2:17100819-17100838 Msa0592030:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 17099245 17101578 17099245 ID=Msa0592030;Name=Msa0592030
chr4_2 mRNA 17099245 17101578 17099245 ID=Msa0592030-mRNA-1;Parent=Msa0592030;Name=Msa0592030-mRNA-1;_AED=0.03;_eAED=0.03;_QI=9|1|1|1|1|1|3|342|185
chr4_2 exon 17099245 17099490 17099245 ID=Msa0592030-mRNA-1:exon:4949;Parent=Msa0592030-mRNA-1
chr4_2 exon 17100801 17100867 17100801 ID=Msa0592030-mRNA-1:exon:4950;Parent=Msa0592030-mRNA-1
chr4_2 exon 17100983 17101578 17100983 ID=Msa0592030-mRNA-1:exon:4951;Parent=Msa0592030-mRNA-1
chr4_2 five_prime_UTR 17099245 17099253 17099245 ID=Msa0592030-mRNA-1:five_prime_utr;Parent=Msa0592030-mRNA-1
chr4_2 CDS 17099254 17099490 17099254 ID=Msa0592030-mRNA-1:cds;Parent=Msa0592030-mRNA-1
chr4_2 CDS 17100801 17100867 17100801 ID=Msa0592030-mRNA-1:cds;Parent=Msa0592030-mRNA-1
chr4_2 CDS 17100983 17101236 17100983 ID=Msa0592030-mRNA-1:cds;Parent=Msa0592030-mRNA-1
chr4_2 three_prime_UTR 17101237 17101578 17101237 ID=Msa0592030-mRNA-1:three_prime_utr;Parent=Msa0592030-mRNA-1
Gene Sequence

>Msa0592030

ATGTTATTATTGTTGGTGACAATCATTTCCAACCTTCACCAACCCATTACCTATACCAACCACAATTCTTTGCTATTCATTCATTTTCACCAACCACCCACCTCTTTTTTTCAAATACTCTCAATTTTTGTGAGTACTGATCAATCAACAATGGGTGAAGAGCGTAAGGTGTTCACTTTGGCTGATGTCTCTAAGCATAACAGTGCCAAAGACTGCTGGCTTGTTATTCATAACAAGGTTTATGATGTGACAAAGTTCTTGGAGGACCACCCTGGTGGAGATGAAGTTCTAATTTCTTCCACAGGGAAAGACGCATCAACTGATTTTGATGATATCGGTCACAGCACAAGTGCTTATACAATGATGGAAGAGTTTTATGTTGGAGACATTGATTCATCAACCATCCCTTCTAAGGTTGATTACACTCCACCGAAACAACCTCACTATAATCAGGACAAGACATCAGAGTTCGTCATCAAGATCCTCCAATTTCTAGTTCCCTTGTTTATCTTGGGTGTGGCAGTTGGTATTCGTTTCTACACCAAATCAACACAATGA
Protein sequence

>Msa0592030

MLLLLVTIISNLHQPITYTNHNSLLFIHFHQPPTSFFQILSIFVSTDQSTMGEERKVFTLADVSKHNSAKDCWLVIHNKVYDVTKFLEDHPGGDEVLISSTGKDASTDFDDIGHSTSAYTMMEEFYVGDIDSSTIPSKVDYTPPKQPHYNQDKTSEFVIKILQFLVPLFILGVAVGIRFYTKSTQ*