AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0207350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0207350 MtrunA17_Chr3g0121191 83.721 215 20 3 3 202 5 219 1.48e-103 304
Msa0207350 MtrunA17_Chr3g0121191 45.833 168 62 5 202 359 13 161 1.36e-24 100
Msa0207350 MtrunA17_Chr8g0391691 53.030 132 47 2 81 197 36 167 7.05e-35 126
Msa0207350 MtrunA17_Chr1g0161211 46.377 138 59 2 81 203 46 183 3.34e-29 111
Msa0207350 MtrunA17_Chr3g0111371 36.765 136 73 3 81 205 5 138 1.72e-20 86.7
Msa0207350 MtrunA17_Chr5g0434951 35.294 136 70 3 81 200 4 137 1.39e-19 84.0
Msa0207350 MtrunA17_Chr4g0057651 39.130 138 66 4 79 200 4 139 2.40e-19 83.6
Msa0207350 MtrunA17_Chr1g0169581 32.117 137 76 2 81 200 4 140 4.09e-19 82.8
Msa0207350 MtrunA17_Chr5g0402331 32.692 156 90 1 61 201 28 183 4.48e-19 84.0
Msa0207350 MtrunA17_Chr4g0057641 37.226 137 68 4 80 200 5 139 8.12e-19 82.0
Msa0207350 MtrunA17_Chr5g0402361 32.716 162 94 3 61 207 28 189 1.51e-18 85.9
Msa0207350 MtrunA17_Chr5g0402361 32.692 156 90 1 61 201 200 355 1.36e-17 83.2
Msa0207350 MtrunA17_Chr8g0392951 33.099 142 81 2 81 208 24 165 1.69e-18 82.0
Msa0207350 MtrunA17_Chr7g0225921 33.333 138 73 2 81 201 6 141 7.84e-18 79.3
Msa0207350 MtrunA17_Chr4g0057661 38.686 137 66 5 80 200 5 139 1.35e-17 78.6
Msa0207350 MtrunA17_Chr1g0169621 30.909 165 72 4 78 200 1 165 6.70e-16 74.7
Msa0207350 MtrunA17_Chr6g0482731 31.655 139 72 3 81 200 26 160 1.84e-15 73.6
Msa0207350 MtrunA17_Chr7g0256701 31.293 147 81 3 78 204 70 216 7.06e-15 72.8
Msa0207350 MtrunA17_Chr8g0351681 33.793 145 73 3 81 202 49 193 7.84e-15 72.4
Msa0207350 MtrunA17_Chr7g0245911 32.639 144 73 3 81 200 47 190 7.94e-15 72.4
Msa0207350 MtrunA17_Chr6g0459231 33.333 132 74 2 84 201 11 142 9.16e-15 70.9
Msa0207350 MtrunA17_Chr1g0188171 28.249 177 97 5 31 204 1 150 1.67e-14 70.5
Msa0207350 MtrunA17_Chr4g0033601 32.168 143 68 6 81 200 5 141 3.21e-13 66.6
Msa0207350 MtrunA17_Chr3g0093271 32.857 140 73 3 81 202 63 199 3.70e-13 67.8
Msa0207350 MtrunA17_Chr3g0093271 45.588 68 37 0 133 200 57 124 1.59e-12 65.9
Msa0207350 MtrunA17_Chr1g0166281 31.387 137 79 2 80 201 11 147 4.88e-13 66.2
Msa0207350 MtrunA17_Chr5g0440931 43.421 76 43 0 133 208 6 81 6.16e-13 65.9
Msa0207350 MtrunA17_Chr5g0440931 32.857 140 73 3 81 202 12 148 1.62e-12 64.7
Msa0207350 MtrunA17_Chr4g0062221 31.973 147 81 4 81 210 11 155 8.32e-13 65.9
Msa0207350 MtrunA17_Chr3g0116151 31.818 132 76 4 83 200 8 139 1.54e-12 64.3
Msa0207350 MtrunA17_Chr7g0254731 45.588 68 37 0 133 200 6 73 1.84e-12 64.3
Msa0207350 MtrunA17_Chr7g0254731 32.143 140 74 3 81 202 12 148 3.54e-12 63.5
Msa0207350 MtrunA17_Chr3g0116141 31.818 132 76 4 83 200 7 138 1.93e-12 64.3
Msa0207350 MtrunA17_Chr6g0460211 32.857 140 73 3 81 202 12 148 2.07e-12 64.3
Msa0207350 MtrunA17_Chr6g0460211 42.105 76 44 0 133 208 6 81 2.22e-12 64.3
Msa0207350 MtrunA17_Chr3g0121181 65.854 41 14 0 161 201 5 45 2.98e-12 60.8
Msa0207350 MtrunA17_Chr3g0125311 27.333 150 82 3 81 203 13 162 3.43e-12 63.9
Msa0207350 MtrunA17_Chr4g0044961 30.147 136 82 2 81 203 57 192 4.12e-12 64.7
Msa0207350 MtrunA17_Chr1g0160151 31.293 147 72 3 81 203 22 163 7.99e-12 63.2
Msa0207350 MtrunA17_Chr8g0336891 42.157 102 38 3 211 291 105 206 1.42e-11 63.5
Msa0207350 MtrunA17_Chr7g0245231 33.663 101 61 1 126 226 17 111 2.23e-11 62.0
Msa0207350 MtrunA17_Chr5g0396261 40.323 62 37 0 138 199 51 112 2.39e-11 60.5
Msa0207350 MtrunA17_Chr6g0450921 33.663 101 61 1 126 226 16 110 2.52e-11 61.6
Msa0207350 MtrunA17_Chr8g0373691 37.500 88 55 0 122 209 36 123 3.68e-11 61.6
Msa0207350 MtrunA17_Chr1g0185001 29.688 128 68 3 76 203 43 148 4.10e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0207350 AT1G24620.1 39.444 180 81 6 49 204 1 176 6.14e-27 105
Msa0207350 AT1G66400.1 35.211 142 75 2 81 205 15 156 3.95e-23 94.7
Msa0207350 AT1G18210.2 34.351 131 75 2 81 200 23 153 8.87e-19 83.2
Msa0207350 AT1G18210.1 34.351 131 75 2 81 200 23 153 8.87e-19 83.2
Msa0207350 AT5G37770.1 32.624 141 72 3 81 200 17 155 1.70e-18 82.0
Msa0207350 AT5G37770.2 32.624 141 72 3 81 200 17 155 1.70e-18 82.0
Msa0207350 AT1G73630.1 32.237 152 87 3 77 217 16 162 2.80e-18 81.6
Msa0207350 AT2G41410.1 36.879 141 71 3 81 203 70 210 7.25e-18 81.6
Msa0207350 AT2G43290.1 35.211 142 70 4 81 200 65 206 1.67e-17 80.9
Msa0207350 AT3G10190.1 35.664 143 72 4 80 204 69 209 6.75e-15 73.2
Msa0207350 AT3G59440.1 32.847 137 76 3 80 200 50 186 7.93e-15 72.8
Msa0207350 AT5G17470.1 30.952 126 68 2 81 206 38 144 1.41e-14 70.9
Msa0207350 AT4G03290.1 35.417 144 73 3 81 204 5 148 2.42e-14 70.5
Msa0207350 AT2G15680.1 33.333 138 74 5 81 203 50 184 4.99e-14 70.5
Msa0207350 AT1G66410.4 33.071 127 64 3 76 202 7 112 7.12e-14 67.8
Msa0207350 AT1G66410.3 33.071 127 64 3 76 202 7 112 7.12e-14 67.8
Msa0207350 AT2G36180.1 33.333 120 62 3 81 200 36 137 1.23e-13 68.2
Msa0207350 AT5G37780.4 33.071 127 64 3 76 202 30 135 1.88e-13 67.4
Msa0207350 AT1G66410.2 33.071 127 64 3 76 202 53 158 3.70e-13 67.0
Msa0207350 AT5G37780.2 33.071 127 64 3 76 202 58 163 3.84e-13 67.4
Msa0207350 AT1G66410.1 33.071 127 64 3 76 202 43 148 4.68e-13 66.6
Msa0207350 AT1G66410.1 43.421 76 43 0 133 208 6 81 7.49e-13 65.9
Msa0207350 AT5G37780.1 33.071 127 64 3 76 202 43 148 4.68e-13 66.6
Msa0207350 AT5G37780.1 43.421 76 43 0 133 208 6 81 7.49e-13 65.9
Msa0207350 AT2G27030.3 33.099 142 74 3 81 204 12 150 5.65e-13 67.0
Msa0207350 AT2G27030.3 45.588 68 37 0 133 200 6 73 6.75e-12 63.9
Msa0207350 AT5G37780.3 33.071 127 64 3 76 202 69 174 5.94e-13 67.0
Msa0207350 AT3G43810.4 31.496 127 66 3 76 202 7 112 6.12e-13 65.1
Msa0207350 AT3G43810.3 31.496 127 66 3 76 202 7 112 6.12e-13 65.1
Msa0207350 AT3G43810.2 32.394 142 75 3 81 204 12 150 9.47e-13 67.4
Msa0207350 AT3G43810.2 45.588 68 37 0 133 200 6 73 6.38e-12 64.7
Msa0207350 AT1G05990.1 47.619 63 31 1 140 200 80 142 1.08e-12 65.5
Msa0207350 AT5G21274.1 33.571 140 72 3 81 202 12 148 1.09e-12 65.5
Msa0207350 AT5G21274.1 45.588 68 37 0 133 200 6 73 3.18e-12 64.3
Msa0207350 AT4G14640.1 32.117 137 76 3 81 201 13 148 1.25e-12 65.5
Msa0207350 AT2G41110.2 31.496 127 66 3 76 202 55 160 1.77e-12 65.1
Msa0207350 AT3G56800.1 32.857 140 73 3 81 202 12 148 2.22e-12 64.7
Msa0207350 AT3G56800.1 42.105 76 44 0 133 208 6 81 2.62e-12 64.3
Msa0207350 AT2G41110.1 32.857 140 73 3 81 202 12 148 2.22e-12 64.7
Msa0207350 AT2G41110.1 42.105 76 44 0 133 208 6 81 2.62e-12 64.3
Msa0207350 AT2G27030.1 32.857 140 73 3 81 202 12 148 2.22e-12 64.7
Msa0207350 AT2G27030.1 42.105 76 44 0 133 208 6 81 2.62e-12 64.3
Msa0207350 AT3G43810.1 32.857 140 73 3 81 202 12 148 2.86e-12 64.3
Msa0207350 AT3G43810.1 42.105 76 44 0 133 208 6 81 3.06e-12 64.3
Msa0207350 AT4G14640.2 28.788 132 73 3 70 201 20 130 9.31e-12 62.4
Msa0207350 AT3G22930.1 29.104 134 74 3 70 203 60 172 1.01e-11 63.5
Msa0207350 AT3G50770.1 30.625 160 88 4 66 202 46 205 1.79e-11 63.5
Msa0207350 AT4G12860.1 33.094 139 72 4 81 200 5 141 2.80e-11 61.6
Msa0207350 AT2G41100.2 30.159 126 82 3 76 200 7 127 6.26e-11 61.6

Find 123 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATAAACTGTTTACCATTT+TGG 0.147897 2_1:+38118891 Msa0207350:CDS
CTATTGTCACTTTGGCCTTA+TGG 0.176207 2_1:+38119115 Msa0207350:CDS
TCATATGATATGGCAATAAT+TGG 0.218393 2_1:+38116456 Msa0207350:five_prime_UTR
CACACATCTATGCCTTAAAA+TGG 0.227910 2_1:+38116228 Msa0207350:five_prime_UTR
GACCTTGCAATCACCTTCTT+TGG 0.242281 2_1:-38119280 None:intergenic
ATGCCTTAAAATGGGGTAAA+TGG 0.252221 2_1:+38116237 Msa0207350:five_prime_UTR
GATGAATGTGAAAGAATTAT+TGG 0.254128 2_1:+38117241 Msa0207350:CDS
TTATTGTATATTTCTTACTA+TGG 0.258217 2_1:+38118971 Msa0207350:CDS
AATTCTTTCACATTCATCTA+AGG 0.287517 2_1:-38117236 None:intergenic
ATCTGATCTAAGAGCGATAA+TGG 0.288423 2_1:+38119308 Msa0207350:CDS
CGCTATACTTAATTTATTAT+CGG 0.293867 2_1:+38116425 Msa0207350:five_prime_UTR
ACGAATTTCTAAAACGAATA+AGG 0.294760 2_1:+38115888 Msa0207350:five_prime_UTR
AGAGGAAATGGAAAACTTAA+AGG 0.303204 2_1:+38117123 Msa0207350:CDS
AATGAAACGTTCAACCCTAA+TGG 0.318380 2_1:-38119019 None:intergenic
TGCTTAGCTCAATAAATTCT+TGG 0.338403 2_1:-38119380 None:intergenic
GTTTACCAAGGCAGCCAAAA+TGG 0.338846 2_1:-38118905 None:intergenic
AATAACCGCGGCGATGAAAT+AGG 0.342707 2_1:-38118953 None:intergenic
CAATGTCAACACATTGGAAT+TGG 0.350732 2_1:+38116925 Msa0207350:CDS
TGTGCCTCTCCATCTCTTGT+AGG 0.355825 2_1:-38115966 None:intergenic
AAAGATGACAACAACGATAA+TGG 0.356762 2_1:-38116867 None:intergenic
CAGCTACGGAGAAACAAAAT+TGG 0.368263 2_1:+38116896 Msa0207350:intron
CTATGAATCTGAAGAGGAAA+TGG 0.373890 2_1:+38117111 Msa0207350:CDS
ACTATTATATTCTTAAAGTT+AGG 0.380920 2_1:+38116491 Msa0207350:five_prime_UTR
CGGATTATCTGAGTTGGAAT+TGG 0.386893 2_1:-38116563 None:intergenic
GGGGTTTCGTTCATATGATA+TGG 0.387148 2_1:+38116446 Msa0207350:five_prime_UTR
ATAGATTGTGTATTGTTGTT+TGG 0.388629 2_1:-38116834 None:intergenic
GCTATACTTAATTTATTATC+GGG 0.388673 2_1:+38116426 Msa0207350:five_prime_UTR
AGGATCTGAAAAGAAATTAC+AGG 0.394754 2_1:-38116384 None:intergenic
ATAGGTCGTATAGTGTTGTT+TGG 0.404071 2_1:-38118935 None:intergenic
ACACATCTATGCCTTAAAAT+GGG 0.407398 2_1:+38116229 Msa0207350:five_prime_UTR
CAATTCCAACTCAGATAATC+CGG 0.409288 2_1:+38116564 Msa0207350:CDS
AAGAGATGGAGAGGCACAAC+TGG 0.409692 2_1:+38115971 Msa0207350:five_prime_UTR
TGGAGAAGGAGAGGGGAAGA+TGG 0.416912 2_1:+38116001 Msa0207350:five_prime_UTR
TGTGCTGGGACAGGAGGAAA+GGG 0.420452 2_1:+38116032 Msa0207350:five_prime_UTR
AAGAGCGATAATGGGAAAGT+TGG 0.422840 2_1:+38119317 Msa0207350:CDS
CGCGTAGAGTATTGTTTAGT+AGG 0.425049 2_1:-38116404 None:intergenic
CAACATTGCTATTGTCACTT+TGG 0.428619 2_1:+38119107 Msa0207350:CDS
ATGAAACGTTCAACCCTAAT+GGG 0.429600 2_1:-38119018 None:intergenic
TGCCGGTGCCGGTGAGGAGA+AGG 0.430263 2_1:+38116063 Msa0207350:five_prime_UTR
CTCCTCACCGGCACCGGCAC+CGG 0.444730 2_1:-38116059 None:intergenic
GTGTGTTACGTGTGTATAAA+CGG 0.444731 2_1:+38116292 Msa0207350:five_prime_UTR
TCCATCTCTTGTAGGTATGA+TGG 0.446655 2_1:-38115958 None:intergenic
ATAACGGGAAAGTTGGATAT+AGG 0.451167 2_1:+38117019 Msa0207350:CDS
GGCGGAAGCGGCCATGATGA+TGG 0.451462 2_1:+38116085 Msa0207350:five_prime_UTR
GATGACGTTGATGGCATTGA+TGG 0.458924 2_1:+38117043 Msa0207350:CDS
AAGATGGAAAAGAGTTGTGC+TGG 0.462265 2_1:+38116017 Msa0207350:five_prime_UTR
TGCTAACAAAACAGCTAAAA+CGG 0.465298 2_1:-38116780 None:intergenic
ACTCAGATAATCCGGCTTCA+AGG 0.465470 2_1:+38116572 Msa0207350:CDS
GAGGAAATGGAAAACTTAAA+GGG 0.465725 2_1:+38117124 Msa0207350:CDS
ATAATGGGAAAGTTGGGTAT+AGG 0.468705 2_1:+38119324 Msa0207350:CDS
ACTCCAGTAATCCAGTTTCA+AGG 0.469240 2_1:+38118708 Msa0207350:intron
AAAAGGATCAAAAGCAATGA+AGG 0.473441 2_1:+38116716 Msa0207350:intron
AAGCGTATTAAGCTCCTTTG+CGG 0.477365 2_1:-38117188 None:intergenic
GCATTGAATTACACAGAAGC+TGG 0.479418 2_1:+38116800 Msa0207350:CDS
CGCCGGTGCCGGTGCCGGTG+AGG 0.481246 2_1:+38116057 Msa0207350:five_prime_UTR
GCCCTTCTCCTCACCGGCAC+CGG 0.486043 2_1:-38116065 None:intergenic
GAGATCGATAACGGGAAAGT+TGG 0.487971 2_1:+38117012 Msa0207350:CDS
GGTGAGGAGAAGGGCGGAAG+CGG 0.489629 2_1:+38116073 Msa0207350:five_prime_UTR
CTCAGATCTGAGATCGATAA+CGG 0.493665 2_1:+38117003 Msa0207350:CDS
TCTGATCTAAGAGCGATAAT+GGG 0.494788 2_1:+38119309 Msa0207350:CDS
AGGGTATGTCACAGAGAGAA+AGG 0.496599 2_1:+38116121 Msa0207350:five_prime_UTR
TCAGATCTGAGATCGATAAC+GGG 0.497355 2_1:+38117004 Msa0207350:CDS
CGAGGTTCTGACCTTGAAGC+CGG 0.497573 2_1:-38116583 None:intergenic
GATATAGGAGATGACGTTGA+TGG 0.498114 2_1:+38117034 Msa0207350:CDS
GAAAAGAGTTGTGCTGGGAC+AGG 0.502407 2_1:+38116023 Msa0207350:five_prime_UTR
AGAGCGATAATGGGAAAGTT+GGG 0.504415 2_1:+38119318 Msa0207350:CDS
GTAAATGGAGCACATCGAAA+TGG 0.505877 2_1:+38116252 Msa0207350:five_prime_UTR
TGATGGAGAGGAAGATGGAA+GGG 0.507602 2_1:+38116102 Msa0207350:five_prime_UTR
AAGCAATGAAGGAAAGTTGA+AGG 0.508377 2_1:+38116727 Msa0207350:CDS
TATAAATACAGTGAGCCATA+AGG 0.509742 2_1:-38119130 None:intergenic
TGAACTAGTGTTTAAAGACT+TGG 0.513250 2_1:+38118858 Msa0207350:CDS
TTGTGCTGGGACAGGAGGAA+AGG 0.515636 2_1:+38116031 Msa0207350:five_prime_UTR
GCCGGTGCCGGTGAGGAGAA+GGG 0.520837 2_1:+38116064 Msa0207350:five_prime_UTR
AATACGCTTATGAGAAGCAT+TGG 0.523714 2_1:+38117202 Msa0207350:CDS
TGAAGCCGGATTATCTGAGT+TGG 0.528924 2_1:-38116569 None:intergenic
CAACACATTGGAATTGGAGA+TGG 0.533055 2_1:+38116931 Msa0207350:CDS
CATGATGATGGAGAGGAAGA+TGG 0.535029 2_1:+38116097 Msa0207350:five_prime_UTR
AAGAAATTGGAGACCAAAGA+AGG 0.535558 2_1:+38119267 Msa0207350:CDS
AAACAGTTTATATAAGATGT+TGG 0.539608 2_1:-38118881 None:intergenic
CATCTTCCTCTCCATCATCA+TGG 0.546407 2_1:-38116096 None:intergenic
ATGATGGAGAGGAAGATGGA+AGG 0.559245 2_1:+38116101 Msa0207350:five_prime_UTR
GAAGAAGAAAGTCATTCTGA+TGG 0.561016 2_1:+38116537 Msa0207350:exon
CTCTTCAGTTTCAGAATGTG+TGG 0.566849 2_1:-38119403 None:intergenic
AGGCATAGATGTGTGACACT+TGG 0.568527 2_1:-38116220 None:intergenic
TTGATAGTGATGGTGATGGT+AGG 0.571325 2_1:+38117269 Msa0207350:CDS
ATCTCTTGTAGGTATGATGG+TGG 0.571897 2_1:-38115955 None:intergenic
ACCATCATACCTACAAGAGA+TGG 0.576746 2_1:+38115957 Msa0207350:five_prime_UTR
CTATACTTAATTTATTATCG+GGG 0.576985 2_1:+38116427 Msa0207350:five_prime_UTR
TGCTGGGACAGGAGGAAAGG+GGG 0.577136 2_1:+38116034 Msa0207350:five_prime_UTR
GACCAAAGAAGGTGATTGCA+AGG 0.582968 2_1:+38119278 Msa0207350:CDS
GTTGCTTGAAATAAGTAGCA+AGG 0.584934 2_1:-38119088 None:intergenic
AACAAACTATGAATCTGAAG+AGG 0.586456 2_1:+38117105 Msa0207350:CDS
GTGTATGACATTGATGGCGA+TGG 0.590310 2_1:+38117157 Msa0207350:CDS
AAGAGTTGTGCTGGGACAGG+AGG 0.590658 2_1:+38116026 Msa0207350:five_prime_UTR
CACACATTCTGAAACTGAAG+AGG 0.599111 2_1:+38119404 Msa0207350:CDS
CAATGTCAATGTCAACACAT+TGG 0.601927 2_1:+38116919 Msa0207350:CDS
GATTACGATGGAGAAGGAGA+GGG 0.604311 2_1:+38115993 Msa0207350:five_prime_UTR
CACATCTATGCCTTAAAATG+GGG 0.607191 2_1:+38116230 Msa0207350:five_prime_UTR
GCTTCCGCCCTTCTCCTCAC+CGG 0.610008 2_1:-38116071 None:intergenic
TTCAAGAAATTGGACACAGA+TGG 0.611437 2_1:+38116962 Msa0207350:CDS
TGAAGGAAAGTTGAAGGACA+TGG 0.612935 2_1:+38116733 Msa0207350:CDS
ATTGGTCGTGTTGATAGTGA+TGG 0.614485 2_1:+38117259 Msa0207350:CDS
AGATGGAAAAGAGTTGTGCT+GGG 0.622598 2_1:+38116018 Msa0207350:five_prime_UTR
GGATTACGATGGAGAAGGAG+AGG 0.626081 2_1:+38115992 Msa0207350:five_prime_UTR
AATTTCTAAAACGAATAAGG+TGG 0.626391 2_1:+38115891 Msa0207350:five_prime_UTR
CGATGGTTTCATCACCGCAA+AGG 0.626557 2_1:+38117174 Msa0207350:CDS
AAGTAGCAAGGCTAAACATG+AGG 0.635088 2_1:-38119076 None:intergenic
AAGCGGCCATGATGATGGAG+AGG 0.635852 2_1:+38116090 Msa0207350:five_prime_UTR
CGTGTTGATAGTGATGGTGA+TGG 0.638546 2_1:+38117265 Msa0207350:CDS
GTGCTGGGACAGGAGGAAAG+GGG 0.645191 2_1:+38116033 Msa0207350:five_prime_UTR
TTTGGAAAGCTTGTTTACCA+AGG 0.645930 2_1:-38118917 None:intergenic
AAATTGGACACAGATGGTGA+TGG 0.649231 2_1:+38116968 Msa0207350:CDS
ATTACGATGGAGAAGGAGAG+GGG 0.658748 2_1:+38115994 Msa0207350:five_prime_UTR
GACGTTGATGGCATTGATGG+AGG 0.660924 2_1:+38117046 Msa0207350:CDS
GATGACAACAACGATAATGG+AGG 0.662741 2_1:-38116864 None:intergenic
CATACCTACAAGAGATGGAG+AGG 0.663238 2_1:+38115962 Msa0207350:five_prime_UTR
CAACTGGATTACGATGGAGA+AGG 0.673416 2_1:+38115987 Msa0207350:five_prime_UTR
GAGGCACAACTGGATTACGA+TGG 0.678852 2_1:+38115981 Msa0207350:five_prime_UTR
ATAGGAGACGATGTTGACAA+CGG 0.711944 2_1:+38119342 Msa0207350:CDS
GGAGACGATGTTGACAACGG+AGG 0.719496 2_1:+38119345 Msa0207350:CDS
TAAGAAATATACAATAACCG+CGG 0.725581 2_1:-38118965 None:intergenic
TACGACCTATTTCATCGCCG+CGG 0.727735 2_1:+38118948 Msa0207350:CDS
GGTGCCGGTGAGGAGAAGGG+CGG 0.730053 2_1:+38116067 Msa0207350:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTACAAAAAAC+CGG - chr2_1:38117809-38117828 None:intergenic 10.0%
!! TATATTGAATTTATTATACA+GGG + chr2_1:38118618-38118637 Msa0207350:intron 10.0%
!! ACTATTATATTCTTAAAGTT+AGG + chr2_1:38116491-38116510 Msa0207350:five_prime_UTR 15.0%
!!! AATCATTTTGTATAAGATTT+TGG - chr2_1:38116759-38116778 None:intergenic 15.0%
!!! AATTGTGTTGTTATTTTTTT+TGG + chr2_1:38117435-38117454 Msa0207350:intron 15.0%
!! CTATATTGAATTTATTATAC+AGG + chr2_1:38118617-38118636 Msa0207350:intron 15.0%
!!! TAACTTGTTTTAATTTTGAA+AGG - chr2_1:38118779-38118798 None:intergenic 15.0%
!! TTATTGTATATTTCTTACTA+TGG + chr2_1:38118971-38118990 Msa0207350:CDS 15.0%
!!! ATATATAATTATCGATTTCA+AGG + chr2_1:38119162-38119181 Msa0207350:intron 15.0%
!! CGCTATACTTAATTTATTAT+CGG + chr2_1:38116425-38116444 Msa0207350:five_prime_UTR 20.0%
!! GCTATACTTAATTTATTATC+GGG + chr2_1:38116426-38116445 Msa0207350:five_prime_UTR 20.0%
!! CTATACTTAATTTATTATCG+GGG + chr2_1:38116427-38116446 Msa0207350:five_prime_UTR 20.0%
!! ATCAAAATTAGATACATACT+AGG + chr2_1:38117871-38117890 Msa0207350:intron 20.0%
!! TCAAAATTAGATACATACTA+GGG + chr2_1:38117872-38117891 Msa0207350:intron 20.0%
!! GATAATCGTTAATTTAGTTA+AGG - chr2_1:38118075-38118094 None:intergenic 20.0%
!! GTTTCTATTAGAAAAAACAA+TGG + chr2_1:38118171-38118190 Msa0207350:intron 20.0%
!! ATGACTTATATTCAAATGTT+TGG + chr2_1:38118428-38118447 Msa0207350:intron 20.0%
!! TGTTGTTAACAAATTGAAAA+AGG + chr2_1:38118463-38118482 Msa0207350:intron 20.0%
!!! TGATGTTTTAGATATTGTTT+AGG - chr2_1:38118497-38118516 None:intergenic 20.0%
!!! TTTTTTTTTTGTTTTCCCTT+TGG + chr2_1:38118818-38118837 Msa0207350:intron 20.0%
!! AAACAGTTTATATAAGATGT+TGG - chr2_1:38118884-38118903 None:intergenic 20.0%
!!! ATATAAACTGTTTACCATTT+TGG + chr2_1:38118891-38118910 Msa0207350:CDS 20.0%
!!! TATTTTAAAACAACCCATTA+GGG + chr2_1:38119005-38119024 Msa0207350:CDS 20.0%
!!! GAAATTCGTTTTTCTCTAAT+CGG - chr2_1:38115877-38115896 None:intergenic 25.0%
!!! ACGAATTTCTAAAACGAATA+AGG + chr2_1:38115888-38115907 Msa0207350:five_prime_UTR 25.0%
!!! AATTTCTAAAACGAATAAGG+TGG + chr2_1:38115891-38115910 Msa0207350:five_prime_UTR 25.0%
! CTTGGCAAAAACAAATTTAA+GGG - chr2_1:38116205-38116224 None:intergenic 25.0%
! ACTTGGCAAAAACAAATTTA+AGG - chr2_1:38116206-38116225 None:intergenic 25.0%
!!! TTTTTTTATGTGAGAGAATC+TGG - chr2_1:38116320-38116339 None:intergenic 25.0%
! TCATATGATATGGCAATAAT+TGG + chr2_1:38116456-38116475 Msa0207350:five_prime_UTR 25.0%
! TTTGAACTTGAAAGGTAAAA+TGG - chr2_1:38116639-38116658 None:intergenic 25.0%
!!! TTTTTTGTTTTCCCTCAAAA+AGG + chr2_1:38116699-38116718 Msa0207350:intron 25.0%
!! ATAGATTGTGTATTGTTGTT+TGG - chr2_1:38116837-38116856 None:intergenic 25.0%
! TCATCTTTATTAATCAGCTA+CGG + chr2_1:38116882-38116901 Msa0207350:intron 25.0%
! AATTCTTTCACATTCATCTA+AGG - chr2_1:38117239-38117258 None:intergenic 25.0%
! GATGAATGTGAAAGAATTAT+TGG + chr2_1:38117241-38117260 Msa0207350:CDS 25.0%
!!! GATTTTAGAATCATGATGAT+GGG + chr2_1:38117304-38117323 Msa0207350:CDS 25.0%
!!! GTGTTGTTATTTTTTTTGGT+TGG + chr2_1:38117439-38117458 Msa0207350:intron 25.0%
! TGTTGATGTTATTCATGATA+TGG + chr2_1:38117478-38117497 Msa0207350:intron 25.0%
! ATGGATCATTAAAAAAAGAG+AGG + chr2_1:38117559-38117578 Msa0207350:intron 25.0%
! GGTGAAGATGTTAAAAAAAA+TGG - chr2_1:38117840-38117859 None:intergenic 25.0%
!!! TAATTTTGATTTGCCAATCA+TGG - chr2_1:38117861-38117880 None:intergenic 25.0%
! TCATGACTAAATAATTGGTT+CGG - chr2_1:38117957-38117976 None:intergenic 25.0%
! CATGTTCATGACTAAATAAT+TGG - chr2_1:38117962-38117981 None:intergenic 25.0%
!!! GACAAGATCAAATTGATTTT+AGG + chr2_1:38118041-38118060 Msa0207350:intron 25.0%
! AATCGTTAATTTAGTTAAGG+TGG - chr2_1:38118072-38118091 None:intergenic 25.0%
! AACAATGGTATAGTAATAGT+TGG + chr2_1:38118186-38118205 Msa0207350:intron 25.0%
! ACAAAAAGAATTGTTTGCAA+TGG - chr2_1:38118392-38118411 None:intergenic 25.0%
!! CAAGTTACTAACTATAGTTT+TGG + chr2_1:38118792-38118811 Msa0207350:intron 25.0%
! TAAGAAATATACAATAACCG+CGG - chr2_1:38118968-38118987 None:intergenic 25.0%
!!! CTATTTTAAAACAACCCATT+AGG + chr2_1:38119004-38119023 Msa0207350:CDS 25.0%
! GGAAAAAATCTTCAAGAAAT+TGG + chr2_1:38119254-38119273 Msa0207350:CDS 25.0%
ACACATCTATGCCTTAAAAT+GGG + chr2_1:38116229-38116248 Msa0207350:five_prime_UTR 30.0%
AGGATCTGAAAAGAAATTAC+AGG - chr2_1:38116387-38116406 None:intergenic 30.0%
!! CTAACTTGTTTGAACTTGAA+AGG - chr2_1:38116647-38116666 None:intergenic 30.0%
!! TTGCTTTTGATCCTTTTTGA+GGG - chr2_1:38116713-38116732 None:intergenic 30.0%
!! ATTGCTTTTGATCCTTTTTG+AGG - chr2_1:38116714-38116733 None:intergenic 30.0%
AAAAGGATCAAAAGCAATGA+AGG + chr2_1:38116716-38116735 Msa0207350:intron 30.0%
TGCTAACAAAACAGCTAAAA+CGG - chr2_1:38116783-38116802 None:intergenic 30.0%
AAAGATGACAACAACGATAA+TGG - chr2_1:38116870-38116889 None:intergenic 30.0%
GGAAAAACTCTTCAAGAAAT+TGG + chr2_1:38116952-38116971 Msa0207350:CDS 30.0%
AACAAACTATGAATCTGAAG+AGG + chr2_1:38117105-38117124 Msa0207350:CDS 30.0%
AGAGGAAATGGAAAACTTAA+AGG + chr2_1:38117123-38117142 Msa0207350:CDS 30.0%
GAGGAAATGGAAAACTTAAA+GGG + chr2_1:38117124-38117143 Msa0207350:CDS 30.0%
! TTTTCTGTGTATGACATTGA+TGG + chr2_1:38117151-38117170 Msa0207350:CDS 30.0%
!!! GGATTTTAGAATCATGATGA+TGG + chr2_1:38117303-38117322 Msa0207350:CDS 30.0%
TTTGATGTTGGACTCTATTA+TGG - chr2_1:38117336-38117355 None:intergenic 30.0%
AGAAATGTCAAGTTTGATGT+TGG - chr2_1:38117348-38117367 None:intergenic 30.0%
TTTAACATCTTCACCATGAT+TGG + chr2_1:38117845-38117864 Msa0207350:intron 30.0%
ACTAGGGATGTATTAGAAAT+AGG + chr2_1:38117888-38117907 Msa0207350:intron 30.0%
GGATGTATTAGAAATAGGTT+AGG + chr2_1:38117893-38117912 Msa0207350:intron 30.0%
TATTAGAAATAGGTTAGGTC+TGG + chr2_1:38117898-38117917 Msa0207350:intron 30.0%
! GTTCGGTCTAATTTAAGATT+GGG - chr2_1:38117940-38117959 None:intergenic 30.0%
GACGTAATACATGTTCAATA+AGG + chr2_1:38118003-38118022 Msa0207350:intron 30.0%
AATAAGGATGAGATGATACA+AGG + chr2_1:38118019-38118038 Msa0207350:intron 30.0%
! TTGCTAATATTTGCCATTTC+TGG + chr2_1:38118102-38118121 Msa0207350:intron 30.0%
! TGCTAATATTTGCCATTTCT+GGG + chr2_1:38118103-38118122 Msa0207350:intron 30.0%
TCATAATTTGAACCCAGAAA+TGG - chr2_1:38118118-38118137 None:intergenic 30.0%
! GTCTTCTTTACTTTATTTCC+TGG + chr2_1:38118553-38118572 Msa0207350:intron 30.0%
GTTATTGAAAAGAAAGTTCC+AGG - chr2_1:38118574-38118593 None:intergenic 30.0%
TCGGAATATATTTATGCAGT+CGG - chr2_1:38118673-38118692 None:intergenic 30.0%
ACTGCATAAATATATTCCGA+TGG + chr2_1:38118673-38118692 Msa0207350:intron 30.0%
!!! TTTTGTTTTCCCTTTGGAAA+AGG + chr2_1:38118824-38118843 Msa0207350:intron 30.0%
TGAACTAGTGTTTAAAGACT+TGG + chr2_1:38118858-38118877 Msa0207350:CDS 30.0%
AGTAAGAGTAAGACTGAAAT+TGG - chr2_1:38119045-38119064 None:intergenic 30.0%
ATTTCAGTCTTACTCTTACT+AGG + chr2_1:38119045-38119064 Msa0207350:intron 30.0%
TATAAATACAGTGAGCCATA+AGG - chr2_1:38119133-38119152 None:intergenic 30.0%
TGCTTAGCTCAATAAATTCT+TGG - chr2_1:38119383-38119402 None:intergenic 30.0%
CACACATCTATGCCTTAAAA+TGG + chr2_1:38116228-38116247 Msa0207350:five_prime_UTR 35.0%
CACATCTATGCCTTAAAATG+GGG + chr2_1:38116230-38116249 Msa0207350:five_prime_UTR 35.0%
ATGCCTTAAAATGGGGTAAA+TGG + chr2_1:38116237-38116256 Msa0207350:five_prime_UTR 35.0%
GTGTGTTACGTGTGTATAAA+CGG + chr2_1:38116292-38116311 Msa0207350:five_prime_UTR 35.0%
GAAGAAGAAAGTCATTCTGA+TGG + chr2_1:38116537-38116556 Msa0207350:exon 35.0%
CAATTCCAACTCAGATAATC+CGG + chr2_1:38116564-38116583 Msa0207350:CDS 35.0%
AAGCAATGAAGGAAAGTTGA+AGG + chr2_1:38116727-38116746 Msa0207350:CDS 35.0%
CAATGTCAATGTCAACACAT+TGG + chr2_1:38116919-38116938 Msa0207350:CDS 35.0%
CAATGTCAACACATTGGAAT+TGG + chr2_1:38116925-38116944 Msa0207350:CDS 35.0%
TTCAAGAAATTGGACACAGA+TGG + chr2_1:38116962-38116981 Msa0207350:CDS 35.0%
! ATAACGGGAAAGTTGGATAT+AGG + chr2_1:38117019-38117038 Msa0207350:CDS 35.0%
CTATGAATCTGAAGAGGAAA+TGG + chr2_1:38117111-38117130 Msa0207350:CDS 35.0%
! AATACGCTTATGAGAAGCAT+TGG + chr2_1:38117202-38117221 Msa0207350:CDS 35.0%
!! TGATGGTAGGATTGATTTTG+AGG + chr2_1:38117282-38117301 Msa0207350:CDS 35.0%
GTAGAATGAATTTGATCCAG+TGG - chr2_1:38117415-38117434 None:intergenic 35.0%
CATGATATGGTATGTAGAAG+TGG + chr2_1:38117491-38117510 Msa0207350:intron 35.0%
ACGAGAGTTAAGTACTAACA+TGG + chr2_1:38117540-38117559 Msa0207350:intron 35.0%
TAGCATGACTGCTTAAAGTT+CGG + chr2_1:38117647-38117666 Msa0207350:intron 35.0%
TCGGTAATTGTCAGAAAACA+CGG + chr2_1:38117666-38117685 Msa0207350:intron 35.0%
ACAGTTATTCGCTTGGTATT+CGG + chr2_1:38117713-38117732 Msa0207350:intron 35.0%
! GGTTCGGTCTAATTTAAGAT+TGG - chr2_1:38117941-38117960 None:intergenic 35.0%
GTCGAGTTCAATTAGATTCA+CGG - chr2_1:38117986-38118005 None:intergenic 35.0%
! TTTTCGAAACCATCGCAAAA+TGG + chr2_1:38118279-38118298 Msa0207350:intron 35.0%
!! GTTGAAAAGTGGATTTGCTA+CGG - chr2_1:38118370-38118389 None:intergenic 35.0%
TGTTTGCAATGGTTGAAAAG+TGG - chr2_1:38118381-38118400 None:intergenic 35.0%
! TATACAGGGCATCATTATTG+TGG + chr2_1:38118632-38118651 Msa0207350:intron 35.0%
TTATTGTGGCCATTTATACC+TGG + chr2_1:38118646-38118665 Msa0207350:intron 35.0%
CGGAATATATTTATGCAGTC+GGG - chr2_1:38118672-38118691 None:intergenic 35.0%
TGGATTACTGGAGTTTGAAT+TGG - chr2_1:38118702-38118721 None:intergenic 35.0%
TAACCTTGAAACTGGATTAC+TGG - chr2_1:38118714-38118733 None:intergenic 35.0%
! TTTGGAAAGCTTGTTTACCA+AGG - chr2_1:38118920-38118939 None:intergenic 35.0%
! ATAGGTCGTATAGTGTTGTT+TGG - chr2_1:38118938-38118957 None:intergenic 35.0%
ATGAAACGTTCAACCCTAAT+GGG - chr2_1:38119021-38119040 None:intergenic 35.0%
AATGAAACGTTCAACCCTAA+TGG - chr2_1:38119022-38119041 None:intergenic 35.0%
GTTGCTTGAAATAAGTAGCA+AGG - chr2_1:38119091-38119110 None:intergenic 35.0%
! CAACATTGCTATTGTCACTT+TGG + chr2_1:38119107-38119126 Msa0207350:CDS 35.0%
TTTCAAGGAGAGAGAAACAA+TGG + chr2_1:38119177-38119196 Msa0207350:intron 35.0%
! TTTTTCCATCTCCAATTCCA+AGG - chr2_1:38116939-38116958 None:intergenic 35.0%
AAGAAATTGGAGACCAAAGA+AGG + chr2_1:38119267-38119286 Msa0207350:CDS 35.0%
ATCTGATCTAAGAGCGATAA+TGG + chr2_1:38119308-38119327 Msa0207350:CDS 35.0%
TCTGATCTAAGAGCGATAAT+GGG + chr2_1:38119309-38119328 Msa0207350:CDS 35.0%
! ATAATGGGAAAGTTGGGTAT+AGG + chr2_1:38119324-38119343 Msa0207350:CDS 35.0%
ATCTCTTGTAGGTATGATGG+TGG - chr2_1:38115958-38115977 None:intergenic 40.0%
ACCATCATACCTACAAGAGA+TGG + chr2_1:38115957-38115976 Msa0207350:five_prime_UTR 40.0%
TCCATCTCTTGTAGGTATGA+TGG - chr2_1:38115961-38115980 None:intergenic 40.0%
AAGATGGAAAAGAGTTGTGC+TGG + chr2_1:38116017-38116036 Msa0207350:five_prime_UTR 40.0%
AGATGGAAAAGAGTTGTGCT+GGG + chr2_1:38116018-38116037 Msa0207350:five_prime_UTR 40.0%
! GCTCCATTTACCCCATTTTA+AGG - chr2_1:38116243-38116262 None:intergenic 40.0%
GTAAATGGAGCACATCGAAA+TGG + chr2_1:38116252-38116271 Msa0207350:five_prime_UTR 40.0%
!! CGCGTAGAGTATTGTTTAGT+AGG - chr2_1:38116407-38116426 None:intergenic 40.0%
GGGGTTTCGTTCATATGATA+TGG + chr2_1:38116446-38116465 Msa0207350:five_prime_UTR 40.0%
! CGGATTATCTGAGTTGGAAT+TGG - chr2_1:38116566-38116585 None:intergenic 40.0%
CAATTCATAGTAGTGAAGCG+AGG - chr2_1:38116604-38116623 None:intergenic 40.0%
TGAAGGAAAGTTGAAGGACA+TGG + chr2_1:38116733-38116752 Msa0207350:CDS 40.0%
GCATTGAATTACACAGAAGC+TGG + chr2_1:38116800-38116819 Msa0207350:CDS 40.0%
! GATGACAACAACGATAATGG+AGG - chr2_1:38116867-38116886 None:intergenic 40.0%
CAGCTACGGAGAAACAAAAT+TGG + chr2_1:38116896-38116915 Msa0207350:intron 40.0%
CAACACATTGGAATTGGAGA+TGG + chr2_1:38116931-38116950 Msa0207350:CDS 40.0%
AAATTGGACACAGATGGTGA+TGG + chr2_1:38116968-38116987 Msa0207350:CDS 40.0%
! CTCAGATCTGAGATCGATAA+CGG + chr2_1:38117003-38117022 Msa0207350:CDS 40.0%
! TCAGATCTGAGATCGATAAC+GGG + chr2_1:38117004-38117023 Msa0207350:CDS 40.0%
GATATAGGAGATGACGTTGA+TGG + chr2_1:38117034-38117053 Msa0207350:CDS 40.0%
AAGCGTATTAAGCTCCTTTG+CGG - chr2_1:38117191-38117210 None:intergenic 40.0%
!! ATTGGTCGTGTTGATAGTGA+TGG + chr2_1:38117259-38117278 Msa0207350:CDS 40.0%
!! TTGATAGTGATGGTGATGGT+AGG + chr2_1:38117269-38117288 Msa0207350:CDS 40.0%
TATCCATAGACAATTGCCAC+TGG + chr2_1:38117396-38117415 Msa0207350:intron 40.0%
CGGTAATTGTCAGAAAACAC+GGG + chr2_1:38117667-38117686 Msa0207350:intron 40.0%
TAATTGTCAGAAAACACGGG+AGG + chr2_1:38117670-38117689 Msa0207350:intron 40.0%
AATTGTCAGAAAACACGGGA+GGG + chr2_1:38117671-38117690 Msa0207350:intron 40.0%
ACCAATCACAGTTATTCGCT+TGG + chr2_1:38117706-38117725 Msa0207350:intron 40.0%
ACCAAGCGAATAACTGTGAT+TGG - chr2_1:38117710-38117729 None:intergenic 40.0%
CAGTTATTCGCTTGGTATTC+GGG + chr2_1:38117714-38117733 Msa0207350:intron 40.0%
CAAGACGAAAACGTTATAGC+CGG + chr2_1:38117787-38117806 Msa0207350:intron 40.0%
GAAATAGGTTAGGTCTGGTT+TGG + chr2_1:38117903-38117922 Msa0207350:intron 40.0%
AACTATCGCAATACTCCATG+TGG + chr2_1:38118241-38118260 Msa0207350:intron 40.0%
GAGTTTGAATTGGACTCCAT+CGG - chr2_1:38118692-38118711 None:intergenic 40.0%
ACTCCAGTAATCCAGTTTCA+AGG + chr2_1:38118708-38118727 Msa0207350:intron 40.0%
CAGTTTCAAGGTTAGTACCT+CGG + chr2_1:38118720-38118739 Msa0207350:intron 40.0%
ATGCATAGTATAGCAAACCG+AGG - chr2_1:38118740-38118759 None:intergenic 40.0%
! GCTTTTGCTCCTTTTCCAAA+GGG - chr2_1:38118836-38118855 None:intergenic 40.0%
AAGTAGCAAGGCTAAACATG+AGG - chr2_1:38119079-38119098 None:intergenic 40.0%
!! CTATTGTCACTTTGGCCTTA+TGG + chr2_1:38119115-38119134 Msa0207350:CDS 40.0%
AGAAACAATGGCATCGCTAA+TGG + chr2_1:38119189-38119208 Msa0207350:intron 40.0%
TGATGACAATAGTAGCACCT+TGG + chr2_1:38119221-38119240 Msa0207350:intron 40.0%
CAATAGTAGCACCTTGGAAT+TGG + chr2_1:38119227-38119246 Msa0207350:intron 40.0%
!! AAGAGCGATAATGGGAAAGT+TGG + chr2_1:38119317-38119336 Msa0207350:CDS 40.0%
!! AGAGCGATAATGGGAAAGTT+GGG + chr2_1:38119318-38119337 Msa0207350:CDS 40.0%
ATAGGAGACGATGTTGACAA+CGG + chr2_1:38119342-38119361 Msa0207350:CDS 40.0%
CTCTTCAGTTTCAGAATGTG+TGG - chr2_1:38119406-38119425 None:intergenic 40.0%
!! CACACATTCTGAAACTGAAG+AGG + chr2_1:38119404-38119423 Msa0207350:CDS 40.0%
CATACCTACAAGAGATGGAG+AGG + chr2_1:38115962-38115981 Msa0207350:five_prime_UTR 45.0%
CAACTGGATTACGATGGAGA+AGG + chr2_1:38115987-38116006 Msa0207350:five_prime_UTR 45.0%
GATTACGATGGAGAAGGAGA+GGG + chr2_1:38115993-38116012 Msa0207350:five_prime_UTR 45.0%
ATTACGATGGAGAAGGAGAG+GGG + chr2_1:38115994-38116013 Msa0207350:five_prime_UTR 45.0%
CATCTTCCTCTCCATCATCA+TGG - chr2_1:38116099-38116118 None:intergenic 45.0%
!! CATGATGATGGAGAGGAAGA+TGG + chr2_1:38116097-38116116 Msa0207350:five_prime_UTR 45.0%
! ATGATGGAGAGGAAGATGGA+AGG + chr2_1:38116101-38116120 Msa0207350:five_prime_UTR 45.0%
TGATGGAGAGGAAGATGGAA+GGG + chr2_1:38116102-38116121 Msa0207350:five_prime_UTR 45.0%
AGGGTATGTCACAGAGAGAA+AGG + chr2_1:38116121-38116140 Msa0207350:five_prime_UTR 45.0%
AGGCATAGATGTGTGACACT+TGG - chr2_1:38116223-38116242 None:intergenic 45.0%
TGAAGCCGGATTATCTGAGT+TGG - chr2_1:38116572-38116591 None:intergenic 45.0%
ACTCAGATAATCCGGCTTCA+AGG + chr2_1:38116572-38116591 Msa0207350:CDS 45.0%
!! GAGATCGATAACGGGAAAGT+TGG + chr2_1:38117012-38117031 Msa0207350:CDS 45.0%
!! GATGACGTTGATGGCATTGA+TGG + chr2_1:38117043-38117062 Msa0207350:CDS 45.0%
!! GTGTATGACATTGATGGCGA+TGG + chr2_1:38117157-38117176 Msa0207350:CDS 45.0%
!! CGTGTTGATAGTGATGGTGA+TGG + chr2_1:38117265-38117284 Msa0207350:CDS 45.0%
GATCCAGTGGCAATTGTCTA+TGG - chr2_1:38117402-38117421 None:intergenic 45.0%
CCATGTGGTAGTTGTAACGA+CGG + chr2_1:38118256-38118275 Msa0207350:intron 45.0%
TATATTTATGCAGTCGGGCC+AGG - chr2_1:38118667-38118686 None:intergenic 45.0%
! CGAGGTACTAACCTTGAAAC+TGG - chr2_1:38118722-38118741 None:intergenic 45.0%
! CGCTTTTGCTCCTTTTCCAA+AGG - chr2_1:38118837-38118856 None:intergenic 45.0%
!!! GTTTACCATTTTGGCTGCCT+TGG + chr2_1:38118900-38118919 Msa0207350:CDS 45.0%
GTTTACCAAGGCAGCCAAAA+TGG - chr2_1:38118908-38118927 None:intergenic 45.0%
AATAACCGCGGCGATGAAAT+AGG - chr2_1:38118956-38118975 None:intergenic 45.0%
TAGCACCTTGGAATTGGAGA+TGG + chr2_1:38119233-38119252 Msa0207350:intron 45.0%
GACCAAAGAAGGTGATTGCA+AGG + chr2_1:38119278-38119297 Msa0207350:CDS 45.0%
GACCTTGCAATCACCTTCTT+TGG - chr2_1:38119283-38119302 None:intergenic 45.0%
TGTGCCTCTCCATCTCTTGT+AGG - chr2_1:38115969-38115988 None:intergenic 50.0%
! AAGAGATGGAGAGGCACAAC+TGG + chr2_1:38115971-38115990 Msa0207350:five_prime_UTR 50.0%
GAGGCACAACTGGATTACGA+TGG + chr2_1:38115981-38116000 Msa0207350:five_prime_UTR 50.0%
GGATTACGATGGAGAAGGAG+AGG + chr2_1:38115992-38116011 Msa0207350:five_prime_UTR 50.0%
GAAAAGAGTTGTGCTGGGAC+AGG + chr2_1:38116023-38116042 Msa0207350:five_prime_UTR 50.0%
!! GACGTTGATGGCATTGATGG+AGG + chr2_1:38117046-38117065 Msa0207350:CDS 50.0%
CGATGGTTTCATCACCGCAA+AGG + chr2_1:38117174-38117193 Msa0207350:CDS 50.0%
CCGTCGTTACAACTACCACA+TGG - chr2_1:38118259-38118278 None:intergenic 50.0%
! GTCGCTAGACCATTTTGCGA+TGG - chr2_1:38118291-38118310 None:intergenic 50.0%
ACACCGATATCCTCGATCGT+TGG + chr2_1:38118309-38118328 Msa0207350:intron 50.0%
CACCGATATCCTCGATCGTT+GGG + chr2_1:38118310-38118329 Msa0207350:intron 50.0%
ACCGATATCCTCGATCGTTG+GGG + chr2_1:38118311-38118330 Msa0207350:intron 50.0%
TCCCCAACGATCGAGGATAT+CGG - chr2_1:38118315-38118334 None:intergenic 50.0%
!! TCGCAATGTGCCATTTGCGA+CGG - chr2_1:38118338-38118357 None:intergenic 50.0%
TACGACCTATTTCATCGCCG+CGG + chr2_1:38118948-38118967 Msa0207350:CDS 50.0%
TGGAGAAGGAGAGGGGAAGA+TGG + chr2_1:38116001-38116020 Msa0207350:five_prime_UTR 55.0%
AAGAGTTGTGCTGGGACAGG+AGG + chr2_1:38116026-38116045 Msa0207350:five_prime_UTR 55.0%
TTGTGCTGGGACAGGAGGAA+AGG + chr2_1:38116031-38116050 Msa0207350:five_prime_UTR 55.0%
TGTGCTGGGACAGGAGGAAA+GGG + chr2_1:38116032-38116051 Msa0207350:five_prime_UTR 55.0%
!! AAGCGGCCATGATGATGGAG+AGG + chr2_1:38116090-38116109 Msa0207350:five_prime_UTR 55.0%
! CGAGGTTCTGACCTTGAAGC+CGG - chr2_1:38116586-38116605 None:intergenic 55.0%
TGTCAGAAAACACGGGAGGG+TGG + chr2_1:38117674-38117693 Msa0207350:intron 55.0%
GCAGTCGGGCCAGGTATAAA+TGG - chr2_1:38118658-38118677 None:intergenic 55.0%
GGAGACGATGTTGACAACGG+AGG + chr2_1:38119345-38119364 Msa0207350:CDS 55.0%
GTGCTGGGACAGGAGGAAAG+GGG + chr2_1:38116033-38116052 Msa0207350:five_prime_UTR 60.0%
TGCTGGGACAGGAGGAAAGG+GGG + chr2_1:38116034-38116053 Msa0207350:five_prime_UTR 60.0%
TCGTTGGGGACCGTCGCAAA+TGG + chr2_1:38118325-38118344 Msa0207350:intron 60.0%
GGCATCGCTAATGGCGACGT+TGG + chr2_1:38119198-38119217 Msa0207350:intron 60.0%
GCTTCCGCCCTTCTCCTCAC+CGG - chr2_1:38116074-38116093 None:intergenic 65.0%
GGTGAGGAGAAGGGCGGAAG+CGG + chr2_1:38116073-38116092 Msa0207350:five_prime_UTR 65.0%
GGCGGAAGCGGCCATGATGA+TGG + chr2_1:38116085-38116104 Msa0207350:five_prime_UTR 65.0%
! GACAGGAGGAAAGGGGGCGC+CGG + chr2_1:38116040-38116059 Msa0207350:five_prime_UTR 70.0%
!! TGCCGGTGCCGGTGAGGAGA+AGG + chr2_1:38116063-38116082 Msa0207350:five_prime_UTR 70.0%
!! GCCGGTGCCGGTGAGGAGAA+GGG + chr2_1:38116064-38116083 Msa0207350:five_prime_UTR 70.0%
GCCCTTCTCCTCACCGGCAC+CGG - chr2_1:38116068-38116087 None:intergenic 70.0%
!! GGTGCCGGTGAGGAGAAGGG+CGG + chr2_1:38116067-38116086 Msa0207350:five_prime_UTR 70.0%
GCGACGGTCCCCAACGATCG+AGG - chr2_1:38118322-38118341 None:intergenic 70.0%
!! AGGAAAGGGGGCGCCGGTGC+CGG + chr2_1:38116046-38116065 Msa0207350:five_prime_UTR 75.0%
!! CTCCTCACCGGCACCGGCAC+CGG - chr2_1:38116062-38116081 None:intergenic 75.0%
!!! CGCCGGTGCCGGTGCCGGTG+AGG + chr2_1:38116057-38116076 Msa0207350:five_prime_UTR 85.0%
!!! GGGGGCGCCGGTGCCGGTGC+CGG + chr2_1:38116052-38116071 Msa0207350:five_prime_UTR 90.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 38115842 38119455 38115842 ID=Msa0207350;Name=Msa0207350
chr2_1 mRNA 38115842 38119455 38115842 ID=Msa0207350-mRNA-1;Parent=Msa0207350;Name=Msa0207350-mRNA-1;_AED=0.09;_eAED=0.10;_QI=714|0.14|0.12|1|0.28|0.12|8|0|359
chr2_1 exon 38115842 38116593 38115842 ID=Msa0207350-mRNA-1:exon:12273;Parent=Msa0207350-mRNA-1
chr2_1 exon 38116721 38116874 38116721 ID=Msa0207350-mRNA-1:exon:12274;Parent=Msa0207350-mRNA-1
chr2_1 exon 38116899 38117086 38116899 ID=Msa0207350-mRNA-1:exon:12275;Parent=Msa0207350-mRNA-1
chr2_1 exon 38117102 38117325 38117102 ID=Msa0207350-mRNA-1:exon:12276;Parent=Msa0207350-mRNA-1
chr2_1 exon 38118723 38118729 38118723 ID=Msa0207350-mRNA-1:exon:12277;Parent=Msa0207350-mRNA-1
chr2_1 exon 38118846 38119033 38118846 ID=Msa0207350-mRNA-1:exon:12278;Parent=Msa0207350-mRNA-1
chr2_1 exon 38119067 38119143 38119067 ID=Msa0207350-mRNA-1:exon:12279;Parent=Msa0207350-mRNA-1
chr2_1 exon 38119252 38119455 38119252 ID=Msa0207350-mRNA-1:exon:12280;Parent=Msa0207350-mRNA-1
chr2_1 five_prime_UTR 38115842 38116555 38115842 ID=Msa0207350-mRNA-1:five_prime_utr;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38116556 38116593 38116556 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38116721 38116874 38116721 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38116899 38117086 38116899 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38117102 38117325 38117102 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38118723 38118729 38118723 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38118846 38119033 38118846 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38119067 38119143 38119067 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
chr2_1 CDS 38119252 38119455 38119252 ID=Msa0207350-mRNA-1:cds;Parent=Msa0207350-mRNA-1
Gene Sequence

>Msa0207350

ATGGTTTCCAATTCCAACTCAGATAATCCGGCTTCAAGGATCAAAAGCAATGAAGGAAAGTTGAAGGACATGGCCAAAATCTTATACAAAATGATTGCCGTTTTAGCTGTTTTGTTAGCATTGAATTACACAGAAGCTGGAAACATGTTTTCCAAACAACAATACACAATCTATTTCATCTCCTCCATTATCCTACGGAGAAACAAAATTGGCAATGTCAATGTCAACACATTGGAATTGGAGATGGAAAAACTCTTCAAGAAATTGGACACAGATGGTGATGGAAAGATATCGATCTCAGATCTGAGATCGATAACGGGAAAGTTGGATATAGGAGATGACGTTGATGGCATTGATGGAGGATCTATCACGCTTCAAGACACAACAAACTATGAATCTGAAGAGGAAATGGAAAACTTAAAGGGCACGTTTTCTGTGTATGACATTGATGGCGATGGTTTCATCACCGCAAAGGAGCTTAATACGCTTATGAGAAGCATTGGTCAAGAGTGTTCCTTAGATGAATGTGAAAGAATTATTGGTCGTGTTGATAGTGATGGTGATGGTAGGATTGATTTTGAGGATTTTAGAATCATGATGATGGTTTCAAGGAGCAAAAGCGATGAACTAGTGTTTAAAGACTTGGCCAACATCTTATATAAACTGTTTACCATTTTGGCTGCCTTGGTAAACAAGCTTTCCAAACAACACTATACGACCTATTTCATCGCCGCGGTTATTGTATATTTCTTACTATGGATTCTTCAAACTATTTTAAAACAACCCATTAGGGTTGAACGATCTGTTGCCTCATGTTTAGCCTTGCTACTTATTTCAAGCAACATTGCTATTGTCACTTTGGCCTTATGGCTCACTATGGAAAAAATCTTCAAGAAATTGGAGACCAAAGAAGGTGATTGCAAGGTCTTGATATCTGATCTAAGAGCGATAATGGGAAAGTTGGGTATAGGAGACGATGTTGACAACGGAGGATCTATCACACTCCAAGAATTTATTGAGCTAAGCACCACACATTCTGAAACTGAAGAGGACATAGAAAACATCAAAGATGTGTTTTGA
Protein sequence

>Msa0207350

MVSNSNSDNPASRIKSNEGKLKDMAKILYKMIAVLAVLLALNYTEAGNMFSKQQYTIYFISSIILRRNKIGNVNVNTLELEMEKLFKKLDTDGDGKISISDLRSITGKLDIGDDVDGIDGGSITLQDTTNYESEEEMENLKGTFSVYDIDGDGFITAKELNTLMRSIGQECSLDECERIIGRVDSDGDGRIDFEDFRIMMMVSRSKSDELVFKDLANILYKLFTILAALVNKLSKQHYTTYFIAAVIVYFLLWILQTILKQPIRVERSVASCLALLLISSNIAIVTLALWLTMEKIFKKLETKEGDCKVLISDLRAIMGKLGIGDDVDNGGSITLQEFIELSTTHSETEEDIENIKDVF*