AlfalfaGEDB | Msa0604610

M. sativa cultivar zhongmu-4 / Msa0604610

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604610	KEH30332.1	96.078	255	9	1	18	271	43	297	7.36e-179	504

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604610	sp\|Q9SZL8\|FRS5_ARATH	54.630	108	45	2	89	192	72	179	1.97e-23	102

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0604610	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0578260	Msa0604610	0.969604	2.284061e-130	-8.615850e-47
Msa0578280	Msa0604610	0.945639	2.093168e-104	-8.615850e-47
Msa0604610	Msa1452170	0.948728	5.304897e-107	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604610	MtrunA17_Chr4g0033611	95.331	257	11	1	16	271	1	257	8.52e-175	482
Msa0604610	MtrunA17_Chr2g0329421	70.388	206	56	4	64	267	1	203	1.57e-100	292
Msa0604610	MtrunA17_Chr3g0078041	43.684	190	97	3	84	269	44	227	1.15e-43	148
Msa0604610	MtrunA17_Chr2g0329361	36.923	195	105	6	82	262	53	243	8.84e-35	125
Msa0604610	MtrunA17_Chr7g0233911	36.559	186	105	4	82	262	26	203	2.99e-33	120
Msa0604610	MtrunA17_Chr5g0448761	50.862	116	52	3	82	192	52	167	1.77e-22	96.7
Msa0604610	MtrunA17_Chr2g0287061	42.202	109	51	1	89	197	182	278	1.99e-21	93.6
Msa0604610	MtrunA17_Chr2g0287061	37.255	102	52	1	89	190	28	117	2.62e-16	78.6
Msa0604610	MtrunA17_Chr1g0203721	36.943	157	87	5	61	208	27	180	4.32e-20	89.7
Msa0604610	MtrunA17_Chr3g0082121	44.860	107	53	3	90	192	43	147	5.85e-20	89.4

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0604610	AT3G59470.2	54.545	264	107	5	18	271	1	261	2.37e-95	281
Msa0604610	AT3G59470.3	63.084	214	69	4	59	271	45	249	3.38e-94	278
Msa0604610	AT3G59470.1	63.084	214	69	4	59	271	45	249	3.38e-94	278
Msa0604610	AT3G07500.1	42.938	177	98	3	89	262	32	208	4.50e-44	149
Msa0604610	AT2G43280.1	38.571	210	105	5	58	262	2	192	2.72e-37	131
Msa0604610	AT4G12850.1	36.264	182	108	3	84	264	5	179	1.66e-32	118
Msa0604610	AT2G43280.2	39.877	163	86	4	105	262	2	157	6.59e-31	113
Msa0604610	AT4G12850.2	41.463	123	69	2	84	205	5	125	7.26e-25	97.1
Msa0604610	AT4G38180.1	54.630	108	45	2	89	192	72	179	2.01e-24	102
Msa0604610	AT4G12850.4	42.056	107	60	1	84	190	5	109	1.02e-23	94.0

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Find 73 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTTTGATACTCCAAAT+TGG	0.289951	4_2:-50253662	Msa0604610:CDS
AAATGTTGCAAAATCAGCTA+TGG	0.513100	4_2:+50251445	None:intergenic
AACGTGACTCAAGATTCTTC+TGG	0.391633	4_2:-50253011	Msa0604610:CDS
AAGACGGAACTGCTATTGGA+CGG	0.612304	4_2:-50252818	Msa0604610:CDS
AATGCATACGCAGCCGAGGT+TGG	0.541461	4_2:-50252879	Msa0604610:CDS
AATGGCGATACAGTAAGAGA+TGG	0.645018	4_2:-50253119	Msa0604610:CDS
ACAAGCGCGAAAAGATTGTG+AGG	0.665448	4_2:-50252758	Msa0604610:CDS
ACTACCAGACGCTTCTGCTG+AGG	0.646967	4_2:-50252955	Msa0604610:CDS
AGAAACAAGGGTTGGTTGCA+GGG	0.508414	4_2:-50252727	Msa0604610:CDS
AGAAGAACATAATCATTGAT+AGG	0.432866	4_2:-50251777	Msa0604610:exon

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACTCCAAATTGGAAAGTA+TGG	-	chr4_2:50251470-50251489	Msa0604610:three_prime_UTR	30.0%
GATATCCATACTTTCCAATT+TGG	+	chr4_2:50251478-50251497	None:intergenic	30.0%
ATTGAAGATGCATATTGCAT+CGG	+	chr4_2:50251572-50251591	None:intergenic	30.0%
AATGATATGTCGTATCTGAA+TGG	+	chr4_2:50251724-50251743	None:intergenic	30.0%
ACATAGAACGATACAGATAT+AGG	+	chr4_2:50251859-50251878	None:intergenic	30.0%
GGTTATTACGAAGTTTGTAA+AGG	-	chr4_2:50252456-50252475	Msa0604610:intron	30.0%
CTTGATAGATTGAGAATGAA+TGG	-	chr4_2:50252649-50252668	Msa0604610:CDS	30.0%
AGTTAATTCTGCATTGAACT+TGG	+	chr4_2:50252864-50252883	None:intergenic	30.0%
TTTATCGCAGAGAGATAAAT+AGG	-	chr4_2:50252912-50252931	Msa0604610:CDS	30.0%
TTAAAAAATGCTCCAAGATG+AGG	-	chr4_2:50253424-50253443	Msa0604610:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4_2	gene	50251452	50253693	50251452	ID=Msa0604610;Name=Msa0604610
chr4_2	mRNA	50251452	50253693	50251452	ID=Msa0604610-mRNA-1;Parent=Msa0604610;Name=Msa0604610-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|0.5\|0.66\|0.66\|1\|1\|3\|329\|271
chr4_2	exon	50251452	50251984	50251452	ID=Msa0604610-mRNA-1:exon:11843;Parent=Msa0604610-mRNA-1
chr4_2	exon	50252608	50253167	50252608	ID=Msa0604610-mRNA-1:exon:11842;Parent=Msa0604610-mRNA-1
chr4_2	exon	50253642	50253693	50253642	ID=Msa0604610-mRNA-1:exon:11841;Parent=Msa0604610-mRNA-1
chr4_2	CDS	50253642	50253693	50253642	ID=Msa0604610-mRNA-1:cds;Parent=Msa0604610-mRNA-1
chr4_2	CDS	50252608	50253167	50252608	ID=Msa0604610-mRNA-1:cds;Parent=Msa0604610-mRNA-1
chr4_2	CDS	50251781	50251984	50251781	ID=Msa0604610-mRNA-1:cds;Parent=Msa0604610-mRNA-1
chr4_2	three_prime_UTR	50251452	50251780	50251452	ID=Msa0604610-mRNA-1:three_prime_utr;Parent=Msa0604610-mRNA-1

Gene Sequence

>Msa0604610

ATGGAATATAAAGCTTTGATACTCCAAATTGGAAAGTATGGATATCTGACACTGGATTTTGAGATTGATGACTCTGTTAATGGCGATACAGTAAGAGATGGTGCCATGGTTGTAGCTGGTAGCATACCTGAGGAATACATAGAAGAAGAAAGTTCTGAACCATCTTACAAACAAGACCAAGATGATAACGTGACTCAAGATTCTTCTGGAGGAGTGTTAACAATACCCGCTGCAGTTCCCACACTACCAGACGCTTCTGCTGAGGAGCCCTATGTGGGTCAGGAGTTCGAGTCGGAAGCAGCAGCGCATGCATTTTACAATGCATACGCAGCCGAGGTTGGATTCATCATACGCGTAAGCAAGCTCTCACGATCAAGGAAAGACGGAACTGCTATTGGACGGGCTCTTGTTTGCAACAAAGAGGGTTACAGAATGCCTGACAAGCGCGAAAAGATTGTGAGGCAAAGGGCAGAAACAAGGGTTGGTTGCAGGGCAATGATTATGATGAGGAAAATTAATTCTGGTAAATGGGTTATTACGAAGTTTGTAAAGGAGCACACACATCCCTTGAATCCTGGAAGTAGTAGAAAGGATATGTTTGAAATTTATCCGTCTCAGAACGAACATGATAAAATCCGCGAACTATCTCAGCAGTTGGCAATAGAGAAAAAGCGATCTGTGACATATAAAAGACAACTTGAAGTGATATTTGATTATATCGAGGAGCATAATGTGGGTCTCTCAAGGAAAATGCAGCGTATAGTAGACAGTGTGAAGGAGATGGAGCCTAAAGAAGAAGAAGAACATAATCATTGA

Protein sequence

>Msa0604610

MEYKALILQIGKYGYLTLDFEIDDSVNGDTVRDGAMVVAGSIPEEYIEEESSEPSYKQDQDDNVTQDSSGGVLTIPAAVPTLPDASAEEPYVGQEFESEAAAHAFYNAYAAEVGFIIRVSKLSRSRKDGTAIGRALVCNKEGYRMPDKREKIVRQRAETRVGCRAMIMMRKINSGKWVITKFVKEHTHPLNPGSSRKDMFEIYPSQNEHDKIRELSQQLAIEKKRSVTYKRQLEVIFDYIEEHNVGLSRKMQRIVDSVKEMEPKEEEEHNH*