AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0578260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0578260 MtrunA17_Chr4g0033611 95.686 255 10 1 37 290 3 257 1.15e-173 480
Msa0578260 MtrunA17_Chr2g0329421 70.388 206 56 4 83 286 1 203 2.41e-100 292
Msa0578260 MtrunA17_Chr3g0078041 43.684 190 97 3 103 288 44 227 1.65e-43 148
Msa0578260 MtrunA17_Chr2g0329361 36.923 195 105 6 101 281 53 243 1.95e-34 125
Msa0578260 MtrunA17_Chr7g0233911 36.559 186 105 4 101 281 26 203 4.40e-33 120
Msa0578260 MtrunA17_Chr5g0448761 50.862 116 52 3 101 211 52 167 3.35e-22 96.7
Msa0578260 MtrunA17_Chr2g0287061 42.202 109 51 1 108 216 182 278 1.90e-21 94.4
Msa0578260 MtrunA17_Chr2g0287061 37.255 102 52 1 108 209 28 117 4.16e-16 78.6
Msa0578260 MtrunA17_Chr3g0082121 44.860 107 53 3 109 211 43 147 3.94e-20 90.5
Msa0578260 MtrunA17_Chr1g0203721 36.943 157 87 5 80 227 27 180 4.05e-20 90.5
Msa0578260 MtrunA17_Chr5g0401121 42.593 108 56 3 109 212 56 161 1.42e-19 83.6
Msa0578260 MtrunA17_Chr6g0458391 38.679 106 64 1 109 213 225 330 3.31e-19 87.8
Msa0578260 MtrunA17_Chr6g0458391 36.283 113 67 1 100 207 77 189 1.50e-15 76.6
Msa0578260 MtrunA17_Chr1g0202341 33.582 134 77 3 83 209 25 153 1.96e-18 85.5
Msa0578260 MtrunA17_Chr5g0440451 39.216 102 50 2 108 209 42 131 1.46e-15 77.0
Msa0578260 MtrunA17_Chr2g0291581 34.328 134 83 2 109 241 71 200 5.34e-15 75.1
Msa0578260 MtrunA17_Chr2g0291591 34.615 104 65 1 108 211 63 163 1.13e-14 73.9
Msa0578260 MtrunA17_Chr3g0080351 29.577 142 85 3 88 214 54 195 2.09e-13 70.5
Msa0578260 MtrunA17_Chr7g0258861 36.364 110 66 3 108 214 9 117 1.13e-12 67.0
Msa0578260 MtrunA17_Chr1g0207551 28.788 132 92 2 82 212 6 136 1.13e-11 65.1
Msa0578260 MtrunA17_Chr2g0329031 32.143 112 70 3 105 214 10 117 3.07e-11 63.9
Msa0578260 MtrunA17_Chr2g0276841 28.111 217 136 10 80 285 42 249 3.65e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0578260 AT3G59470.2 54.545 264 107 5 37 290 1 261 1.24e-94 280
Msa0578260 AT3G59470.3 63.084 214 69 4 78 290 45 249 5.14e-94 278
Msa0578260 AT3G59470.1 63.084 214 69 4 78 290 45 249 5.14e-94 278
Msa0578260 AT3G07500.1 42.938 177 98 3 108 281 32 208 1.66e-43 148
Msa0578260 AT2G43280.1 38.571 210 105 5 77 281 2 192 4.99e-37 131
Msa0578260 AT4G12850.1 36.264 182 108 3 103 283 5 179 2.47e-32 118
Msa0578260 AT2G43280.2 39.877 163 86 4 124 281 2 157 1.01e-30 113
Msa0578260 AT4G12850.2 41.463 123 69 2 103 224 5 125 7.84e-25 97.4
Msa0578260 AT4G38180.1 54.630 108 45 2 108 211 72 179 3.11e-24 102
Msa0578260 AT4G12850.4 42.056 107 60 1 103 209 5 109 1.19e-23 94.4
Msa0578260 AT5G18960.2 36.879 141 63 2 69 209 17 131 2.13e-22 97.4
Msa0578260 AT5G18960.2 37.963 108 55 1 105 212 207 302 1.31e-19 89.4
Msa0578260 AT5G18960.1 36.879 141 63 2 69 209 17 131 2.13e-22 97.4
Msa0578260 AT5G18960.1 37.963 108 55 1 105 212 207 302 1.31e-19 89.4
Msa0578260 AT3G06250.2 38.793 116 59 1 106 221 187 290 6.43e-21 93.2
Msa0578260 AT3G06250.2 40.566 106 51 1 105 210 24 117 3.03e-20 91.3
Msa0578260 AT3G06250.1 38.793 116 59 1 106 221 187 290 6.43e-21 93.2
Msa0578260 AT3G06250.1 40.566 106 51 1 105 210 24 117 3.03e-20 91.3
Msa0578260 AT3G06250.3 38.793 116 59 1 106 221 187 290 6.43e-21 93.2
Msa0578260 AT3G06250.3 40.566 106 51 1 105 210 24 117 3.03e-20 91.3
Msa0578260 AT1G52520.1 39.604 101 58 1 109 209 85 182 2.83e-17 82.4
Msa0578260 AT1G80010.1 37.736 106 63 1 106 211 92 194 3.61e-16 79.0
Msa0578260 AT2G32250.5 38.182 110 62 2 104 211 33 138 5.49e-15 75.5
Msa0578260 AT2G32250.6 38.182 110 62 2 104 211 33 138 5.54e-15 75.5
Msa0578260 AT2G32250.1 38.182 110 62 2 104 211 33 138 5.54e-15 75.5
Msa0578260 AT2G32250.3 38.182 110 62 2 104 211 33 138 5.54e-15 75.5
Msa0578260 AT2G32250.2 38.182 110 62 2 104 211 33 138 5.75e-15 75.5
Msa0578260 AT2G32250.4 38.182 110 62 2 104 211 33 138 5.75e-15 75.5
Msa0578260 AT4G19990.3 28.966 145 75 2 112 242 24 154 2.89e-14 73.6
Msa0578260 AT4G19990.4 28.966 145 75 2 112 242 24 154 2.89e-14 73.6
Msa0578260 AT4G19990.6 28.966 145 75 2 112 242 24 154 2.95e-14 73.6
Msa0578260 AT4G19990.2 28.966 145 75 2 112 242 24 154 2.95e-14 73.6
Msa0578260 AT4G19990.7 28.966 145 75 2 112 242 38 168 3.05e-14 73.2
Msa0578260 AT4G19990.5 28.966 145 75 2 112 242 24 154 3.23e-14 73.2
Msa0578260 AT2G32250.7 39.000 100 55 2 114 211 2 97 5.36e-14 72.4
Msa0578260 AT2G32250.8 39.000 100 55 2 114 211 2 97 5.82e-14 72.4
Msa0578260 AT3G22170.2 29.752 121 71 2 108 214 69 189 3.03e-11 64.3
Msa0578260 AT3G22170.1 29.752 121 71 2 108 214 69 189 3.03e-11 64.3

Find 78 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAAATTAATTCTGGTAAA+TGG 0.122359 4_1:-82823022 Msa0578260:CDS
TTATGTTCTTCTTCTTCTTT+AGG 0.197944 4_1:+82822121 None:intergenic
GAGATTGATGACTCTGTTAA+TGG 0.238281 4_1:-82823470 Msa0578260:CDS
TCTGTATCGTTCTATGTATC+TGG 0.244963 4_1:-82823594 Msa0578260:CDS
CAGAAACAAGGGTTGGTTGC+AGG 0.300475 4_1:-82823061 Msa0578260:CDS
TCTATGTATCTGGGCTTCTT+AGG 0.304144 4_1:-82823584 Msa0578260:intron
CAATTGTGATAACTCATAAA+AGG 0.333301 4_1:-82821832 Msa0578260:three_prime_UTR
GAAAATTAATTCTGGTAAAT+GGG 0.333770 4_1:-82823021 Msa0578260:CDS
ATGATGAGGAAAATTAATTC+TGG 0.337234 4_1:-82823029 Msa0578260:CDS
ATATCCTTTCTACTACTTCC+AGG 0.355137 4_1:+82822957 None:intergenic
ATATGTTTGTTAACGTATCT+TGG 0.363970 4_1:-82823656 Msa0578260:CDS
AGGAAAGACGGAACTGCTAT+TGG 0.388968 4_1:-82823155 Msa0578260:CDS
AACGTGACTCAAGATTCTTC+TGG 0.391633 4_1:-82823344 Msa0578260:CDS
CTTGAGTCACGTTATCATCT+TGG 0.395456 4_1:+82823354 None:intergenic
TCTTGGTCTTGTTTGTAAGA+TGG 0.422563 4_1:+82823371 None:intergenic
TCTACTACTTCCAGGATTCA+AGG 0.432111 4_1:+82822965 None:intergenic
TATTATTGCACACCTCATCT+TGG 0.432815 4_1:+82822020 None:intergenic
AGAAGAACATAATCATTGAT+AGG 0.432866 4_1:-82822110 Msa0578260:exon
GGATATGTTTGAAATTTATC+CGG 0.433360 4_1:-82822940 Msa0578260:intron
TGAGGAGCCCTATGTGGGTC+AGG 0.445713 4_1:-82823270 Msa0578260:CDS
TTGTTAACGTATCTTGGTCA+TGG 0.453450 4_1:-82823650 Msa0578260:CDS
TCTTCTTCTATGTATTCCTC+AGG 0.455153 4_1:+82823404 None:intergenic
GGAATAATTAAGCGCTATCA+AGG 0.466188 4_1:+82821799 None:intergenic
ACATAGAACGATACAGATAT+AGG 0.468123 4_1:+82823600 None:intergenic
TAAGCAAGCTCTCACGATCA+AGG 0.471737 4_1:-82823175 Msa0578260:CDS
CACACACATCCCTTGAATCC+TGG 0.472295 4_1:-82822975 Msa0578260:CDS
TGAGTCTATTCATATGAAGT+TGG 0.472796 4_1:-82821861 Msa0578260:three_prime_UTR
GGCTCCTCAGCAGAAGCGTC+TGG 0.483303 4_1:+82823284 None:intergenic
TTCTGCTGAGGAGCCCTATG+TGG 0.483552 4_1:-82823276 Msa0578260:CDS
TGTTAACGTATCTTGGTCAT+GGG 0.484552 4_1:-82823649 Msa0578260:CDS
CCAACTGCTGAGATAGTTCG+CGG 0.495662 4_1:+82822272 None:intergenic
CTGTATCGTTCTATGTATCT+GGG 0.499393 4_1:-82823593 Msa0578260:CDS
CTACTACTTCCAGGATTCAA+GGG 0.508347 4_1:+82822966 None:intergenic
AGAAACAAGGGTTGGTTGCA+GGG 0.508414 4_1:-82823060 Msa0578260:CDS
AAATGTTGCAAAATCAGCTA+TGG 0.513100 4_1:+82821778 None:intergenic
GGTAGTGTGGGAACTGCAGC+GGG 0.515950 4_1:+82823305 None:intergenic
GGTTATTACGAAGTTTGTAA+AGG 0.521568 4_1:-82823000 Msa0578260:CDS
AGCAGAAGCGTCTGGTAGTG+TGG 0.524624 4_1:+82823292 None:intergenic
AATGCATACGCAGCCGAGGT+TGG 0.541461 4_1:-82823212 Msa0578260:CDS
CGAAAAGATTGTGAGGCAAA+GGG 0.544339 4_1:-82823084 Msa0578260:CDS
GCGAAAAGATTGTGAGGCAA+AGG 0.552809 4_1:-82823085 Msa0578260:CDS
AGCATAATGTGGGTCTCTCA+AGG 0.559262 4_1:-82822183 Msa0578260:CDS
GGCTCTTGTTTGCAACAAAG+AGG 0.560233 4_1:-82823129 Msa0578260:CDS
TATCTAATAACTGCTGACAT+AGG 0.563093 4_1:-82821999 Msa0578260:three_prime_UTR
TGGTAGTGTGGGAACTGCAG+CGG 0.563388 4_1:+82823304 None:intergenic
TCGAACTCCTGACCCACATA+GGG 0.565037 4_1:+82823263 None:intergenic
CTCGAACTCCTGACCCACAT+AGG 0.567393 4_1:+82823262 None:intergenic
AGTAGACAGTGTGAAGGAGA+TGG 0.569154 4_1:-82822146 Msa0578260:CDS
GAATCCTGGAAGTAGTAGAA+AGG 0.569556 4_1:-82822961 Msa0578260:CDS
GATGGTGCCATGGTTGTAGC+TGG 0.569629 4_1:-82823434 Msa0578260:CDS
CCGCGAACTATCTCAGCAGT+TGG 0.570583 4_1:-82822272 Msa0578260:CDS
GCTCTTGTTTGCAACAAAGA+GGG 0.580043 4_1:-82823128 Msa0578260:CDS
TGAGGCAAAGGGCAGAAACA+AGG 0.583136 4_1:-82823073 Msa0578260:CDS
GCAGAAGCGTCTGGTAGTGT+GGG 0.584434 4_1:+82823293 None:intergenic
AGACGGAACTGCTATTGGAC+GGG 0.589838 4_1:-82823150 Msa0578260:CDS
CTCTCACGATCAAGGAAAGA+CGG 0.590017 4_1:-82823167 Msa0578260:CDS
TCTGCTGAGGAGCCCTATGT+GGG 0.595284 4_1:-82823275 Msa0578260:CDS
TTAGTATTAATATGATACAA+TGG 0.596314 4_1:-82821967 Msa0578260:three_prime_UTR
TAACGTATCTTGGTCATGGG+CGG 0.609675 4_1:-82823646 Msa0578260:CDS
AAGACGGAACTGCTATTGGA+CGG 0.612304 4_1:-82823151 Msa0578260:CDS
CTTCATATGAATAGACTCAA+AGG 0.619140 4_1:+82821865 None:intergenic
AGTGATATTTGATTATATCG+AGG 0.622955 4_1:-82822206 Msa0578260:CDS
CAAAGGGCAGAAACAAGGGT+TGG 0.628129 4_1:-82823068 Msa0578260:CDS
TTACAATGCATACGCAGCCG+AGG 0.629238 4_1:-82823216 Msa0578260:CDS
TATGCTACCAGCTACAACCA+TGG 0.631572 4_1:+82823427 None:intergenic
GCGTATAGTAGACAGTGTGA+AGG 0.633138 4_1:-82822152 Msa0578260:CDS
TACAGTAAGAGATGGTGCCA+TGG 0.635293 4_1:-82823444 Msa0578260:CDS
TTATATCGAGGAGCATAATG+TGG 0.635828 4_1:-82822194 Msa0578260:CDS
TATATCGAGGAGCATAATGT+GGG 0.635920 4_1:-82822193 Msa0578260:CDS
AATGGCGATACAGTAAGAGA+TGG 0.645018 4_1:-82823452 Msa0578260:CDS
ACTACCAGACGCTTCTGCTG+AGG 0.646967 4_1:-82823288 Msa0578260:CDS
GAGGCAAAGGGCAGAAACAA+GGG 0.664007 4_1:-82823072 Msa0578260:CDS
GCAGGGCAATGATTATGATG+AGG 0.664420 4_1:-82823043 Msa0578260:CDS
ACAAGCGCGAAAAGATTGTG+AGG 0.665448 4_1:-82823091 Msa0578260:CDS
GTGACTCAAGATTCTTCTGG+AGG 0.668755 4_1:-82823341 Msa0578260:CDS
TGTGGGTCAGGAGTTCGAGT+CGG 0.681569 4_1:-82823258 Msa0578260:CDS
TGTAGCTGGTAGCATACCTG+AGG 0.687245 4_1:-82823420 Msa0578260:CDS
GCGTATGATGAATCCAACCT+CGG 0.694329 4_1:+82823199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATTTATACAATTCTT+CGG + chr4_1:82822548-82822567 None:intergenic 15.0%
!! AAACGAAAATTAAAAATGAA+TGG + chr4_1:82822790-82822809 None:intergenic 15.0%
!! TAAAAATCTAGATAATTGAA+AGG + chr4_1:82823359-82823378 None:intergenic 15.0%
!! TTAGTATTAATATGATACAA+TGG - chr4_1:82823472-82823491 Msa0578260:CDS 15.0%
!! GTATAATTTATACAATTCTT+CGG + chr4_1:82822548-82822567 None:intergenic 15.0%
!! AAACGAAAATTAAAAATGAA+TGG + chr4_1:82822790-82822809 None:intergenic 15.0%
!! TAAAAATCTAGATAATTGAA+AGG + chr4_1:82823359-82823378 None:intergenic 15.0%
!! TTAGTATTAATATGATACAA+TGG - chr4_1:82823472-82823491 Msa0578260:CDS 15.0%
!! GAAAATTAATTCTGGTAAAT+GGG - chr4_1:82822418-82822437 Msa0578260:intron 20.0%
!! AAAGTAACAATGAAAATCAA+CGG + chr4_1:82822609-82822628 None:intergenic 20.0%
!!! TGAAGTTTTTAGGATAATAT+TGG - chr4_1:82822695-82822714 Msa0578260:intron 20.0%
!!! AATGGATGAAGTATTATATA+AGG + chr4_1:82822772-82822791 None:intergenic 20.0%
!!! TAAGTAGTTAAAGGTATTAT+AGG - chr4_1:82822931-82822950 Msa0578260:intron 20.0%
!!! TTCTTGTTATGTAGTTATAT+TGG - chr4_1:82822965-82822984 Msa0578260:CDS 20.0%
!!! CAACTTAGAAATTACTTTTA+CGG - chr4_1:82823017-82823036 Msa0578260:CDS 20.0%
!! GAAAATTAATTCTGGTAAAT+GGG - chr4_1:82822418-82822437 Msa0578260:intron 20.0%
!! AAAGTAACAATGAAAATCAA+CGG + chr4_1:82822609-82822628 None:intergenic 20.0%
!!! TGAAGTTTTTAGGATAATAT+TGG - chr4_1:82822695-82822714 Msa0578260:intron 20.0%
!!! AATGGATGAAGTATTATATA+AGG + chr4_1:82822772-82822791 None:intergenic 20.0%
!!! TAAGTAGTTAAAGGTATTAT+AGG - chr4_1:82822931-82822950 Msa0578260:intron 20.0%
!!! TTCTTGTTATGTAGTTATAT+TGG - chr4_1:82822965-82822984 Msa0578260:CDS 20.0%
!!! CAACTTAGAAATTACTTTTA+CGG - chr4_1:82823017-82823036 Msa0578260:CDS 20.0%
! ATGATGAGGAAAATTAATTC+TGG - chr4_1:82822410-82822429 Msa0578260:intron 25.0%
! GGAAAATTAATTCTGGTAAA+TGG - chr4_1:82822417-82822436 Msa0578260:intron 25.0%
!! GGATATGTTTGAAATTTATC+CGG - chr4_1:82822499-82822518 Msa0578260:intron 25.0%
! GATTCTCGTATATATTGTAA+TGG - chr4_1:82822577-82822596 Msa0578260:intron 25.0%
!! GAATGAATGGTATTAGTTTA+AGG - chr4_1:82822645-82822664 Msa0578260:intron 25.0%
!! CTAAAAACTTCATTCTGAAA+TGG + chr4_1:82822687-82822706 None:intergenic 25.0%
!! CATTTCAGAATGAAGTTTTT+AGG - chr4_1:82822685-82822704 Msa0578260:intron 25.0%
! ATGAGATGATAAGTAGTTAA+AGG - chr4_1:82822922-82822941 Msa0578260:intron 25.0%
! AGTGATATTTGATTATATCG+AGG - chr4_1:82823233-82823252 Msa0578260:CDS 25.0%
! TTATGTTCTTCTTCTTCTTT+AGG + chr4_1:82823321-82823340 None:intergenic 25.0%
! AGAAGAACATAATCATTGAT+AGG - chr4_1:82823329-82823348 Msa0578260:CDS 25.0%
!! CTATTTTCAATCGAATGTAT+AGG - chr4_1:82823550-82823569 Msa0578260:intron 25.0%
! CAATTGTGATAACTCATAAA+AGG - chr4_1:82823607-82823626 Msa0578260:CDS 25.0%
! ATGATGAGGAAAATTAATTC+TGG - chr4_1:82822410-82822429 Msa0578260:intron 25.0%
! GGAAAATTAATTCTGGTAAA+TGG - chr4_1:82822417-82822436 Msa0578260:intron 25.0%
!! GGATATGTTTGAAATTTATC+CGG - chr4_1:82822499-82822518 Msa0578260:intron 25.0%
! GATTCTCGTATATATTGTAA+TGG - chr4_1:82822577-82822596 Msa0578260:intron 25.0%
!! GAATGAATGGTATTAGTTTA+AGG - chr4_1:82822645-82822664 Msa0578260:intron 25.0%
!! CTAAAAACTTCATTCTGAAA+TGG + chr4_1:82822687-82822706 None:intergenic 25.0%
!! CATTTCAGAATGAAGTTTTT+AGG - chr4_1:82822685-82822704 Msa0578260:intron 25.0%
! ATGAGATGATAAGTAGTTAA+AGG - chr4_1:82822922-82822941 Msa0578260:intron 25.0%
! AGTGATATTTGATTATATCG+AGG - chr4_1:82823233-82823252 Msa0578260:CDS 25.0%
! TTATGTTCTTCTTCTTCTTT+AGG + chr4_1:82823321-82823340 None:intergenic 25.0%
! AGAAGAACATAATCATTGAT+AGG - chr4_1:82823329-82823348 Msa0578260:CDS 25.0%
!! CTATTTTCAATCGAATGTAT+AGG - chr4_1:82823550-82823569 Msa0578260:intron 25.0%
! CAATTGTGATAACTCATAAA+AGG - chr4_1:82823607-82823626 Msa0578260:CDS 25.0%
ACATAGAACGATACAGATAT+AGG + chr4_1:82821842-82821861 None:intergenic 30.0%
! CTATAAGATTTTCAGTGTTC+AGG + chr4_1:82821921-82821940 None:intergenic 30.0%
GGTTATTACGAAGTTTGTAA+AGG - chr4_1:82822439-82822458 Msa0578260:intron 30.0%
CTTGATAGATTGAGAATGAA+TGG - chr4_1:82822632-82822651 Msa0578260:intron 30.0%
AGTTAATTCTGCATTGAACT+TGG + chr4_1:82822847-82822866 None:intergenic 30.0%
TTTATCGCAGAGAGATAAAT+AGG - chr4_1:82822895-82822914 Msa0578260:intron 30.0%
TTAAAAAATGCTCCAAGATG+AGG - chr4_1:82823407-82823426 Msa0578260:CDS 30.0%
TATCTAATAACTGCTGACAT+AGG - chr4_1:82823440-82823459 Msa0578260:CDS 30.0%
CTTCATATGAATAGACTCAA+AGG + chr4_1:82823577-82823596 None:intergenic 30.0%
TGAGTCTATTCATATGAAGT+TGG - chr4_1:82823578-82823597 Msa0578260:intron 30.0%
ACATAGAACGATACAGATAT+AGG + chr4_1:82821842-82821861 None:intergenic 30.0%
! CTATAAGATTTTCAGTGTTC+AGG + chr4_1:82821921-82821940 None:intergenic 30.0%
GGTTATTACGAAGTTTGTAA+AGG - chr4_1:82822439-82822458 Msa0578260:intron 30.0%
CTTGATAGATTGAGAATGAA+TGG - chr4_1:82822632-82822651 Msa0578260:intron 30.0%
AGTTAATTCTGCATTGAACT+TGG + chr4_1:82822847-82822866 None:intergenic 30.0%
TTTATCGCAGAGAGATAAAT+AGG - chr4_1:82822895-82822914 Msa0578260:intron 30.0%
TTAAAAAATGCTCCAAGATG+AGG - chr4_1:82823407-82823426 Msa0578260:CDS 30.0%
TATCTAATAACTGCTGACAT+AGG - chr4_1:82823440-82823459 Msa0578260:CDS 30.0%
CTTCATATGAATAGACTCAA+AGG + chr4_1:82823577-82823596 None:intergenic 30.0%
TGAGTCTATTCATATGAAGT+TGG - chr4_1:82823578-82823597 Msa0578260:intron 30.0%
TTGTTAACGTATCTTGGTCA+TGG - chr4_1:82821789-82821808 Msa0578260:three_prime_UTR 35.0%
TGTTAACGTATCTTGGTCAT+GGG - chr4_1:82821790-82821809 Msa0578260:three_prime_UTR 35.0%
TCTGTATCGTTCTATGTATC+TGG - chr4_1:82821845-82821864 Msa0578260:three_prime_UTR 35.0%
CTGTATCGTTCTATGTATCT+GGG - chr4_1:82821846-82821865 Msa0578260:three_prime_UTR 35.0%
! GAGATTGATGACTCTGTTAA+TGG - chr4_1:82821969-82821988 Msa0578260:three_prime_UTR 35.0%
TCTTCTTCTATGTATTCCTC+AGG + chr4_1:82822038-82822057 None:intergenic 35.0%
!! TCTTGGTCTTGTTTGTAAGA+TGG + chr4_1:82822071-82822090 None:intergenic 35.0%
ATATCCTTTCTACTACTTCC+AGG + chr4_1:82822485-82822504 None:intergenic 35.0%
AGAAACAAACGAGGTAAGTA+GGG + chr4_1:82823117-82823136 None:intergenic 35.0%
TAGAAACAAACGAGGTAAGT+AGG + chr4_1:82823118-82823137 None:intergenic 35.0%
! TTATATCGAGGAGCATAATG+TGG - chr4_1:82823245-82823264 Msa0578260:CDS 35.0%
! TATATCGAGGAGCATAATGT+GGG - chr4_1:82823246-82823265 Msa0578260:CDS 35.0%
TATTATTGCACACCTCATCT+TGG + chr4_1:82823422-82823441 None:intergenic 35.0%
GGAATAATTAAGCGCTATCA+AGG + chr4_1:82823643-82823662 None:intergenic 35.0%
TTGTTAACGTATCTTGGTCA+TGG - chr4_1:82821789-82821808 Msa0578260:three_prime_UTR 35.0%
TGTTAACGTATCTTGGTCAT+GGG - chr4_1:82821790-82821809 Msa0578260:three_prime_UTR 35.0%
TCTGTATCGTTCTATGTATC+TGG - chr4_1:82821845-82821864 Msa0578260:three_prime_UTR 35.0%
CTGTATCGTTCTATGTATCT+GGG - chr4_1:82821846-82821865 Msa0578260:three_prime_UTR 35.0%
! GAGATTGATGACTCTGTTAA+TGG - chr4_1:82821969-82821988 Msa0578260:three_prime_UTR 35.0%
TCTTCTTCTATGTATTCCTC+AGG + chr4_1:82822038-82822057 None:intergenic 35.0%
!! TCTTGGTCTTGTTTGTAAGA+TGG + chr4_1:82822071-82822090 None:intergenic 35.0%
ATATCCTTTCTACTACTTCC+AGG + chr4_1:82822485-82822504 None:intergenic 35.0%
AGAAACAAACGAGGTAAGTA+GGG + chr4_1:82823117-82823136 None:intergenic 35.0%
TAGAAACAAACGAGGTAAGT+AGG + chr4_1:82823118-82823137 None:intergenic 35.0%
! TTATATCGAGGAGCATAATG+TGG - chr4_1:82823245-82823264 Msa0578260:CDS 35.0%
! TATATCGAGGAGCATAATGT+GGG - chr4_1:82823246-82823265 Msa0578260:CDS 35.0%
TATTATTGCACACCTCATCT+TGG + chr4_1:82823422-82823441 None:intergenic 35.0%
GGAATAATTAAGCGCTATCA+AGG + chr4_1:82823643-82823662 None:intergenic 35.0%
TCTATGTATCTGGGCTTCTT+AGG - chr4_1:82821855-82821874 Msa0578260:three_prime_UTR 40.0%
!!! CAGATCTTTTGTTGTTGCAG+TGG - chr4_1:82821941-82821960 Msa0578260:three_prime_UTR 40.0%
AATGGCGATACAGTAAGAGA+TGG - chr4_1:82821987-82822006 Msa0578260:three_prime_UTR 40.0%
CTTGAGTCACGTTATCATCT+TGG + chr4_1:82822088-82822107 None:intergenic 40.0%
AACGTGACTCAAGATTCTTC+TGG - chr4_1:82822095-82822114 Msa0578260:exon 40.0%
! GCTCTTGTTTGCAACAAAGA+GGG - chr4_1:82822311-82822330 Msa0578260:intron 40.0%
CGAAAAGATTGTGAGGCAAA+GGG - chr4_1:82822355-82822374 Msa0578260:intron 40.0%
CTACTACTTCCAGGATTCAA+GGG + chr4_1:82822476-82822495 None:intergenic 40.0%
TCTACTACTTCCAGGATTCA+AGG + chr4_1:82822477-82822496 None:intergenic 40.0%
GAATCCTGGAAGTAGTAGAA+AGG - chr4_1:82822478-82822497 Msa0578260:intron 40.0%
! GAAAAAGCATAGGAACTGAC+CGG + chr4_1:82822521-82822540 None:intergenic 40.0%
! CTTCGGTAGTGAAAAAGCAT+AGG + chr4_1:82822531-82822550 None:intergenic 40.0%
TTTCTCTCGTGGAAAGGATA+AGG + chr4_1:82822994-82823013 None:intergenic 40.0%
TTGCAATTTCTCTCGTGGAA+AGG + chr4_1:82823000-82823019 None:intergenic 40.0%
CTAAGTTGCAATTTCTCTCG+TGG + chr4_1:82823005-82823024 None:intergenic 40.0%
AAACAAACGAGGTAAGTAGG+GGG + chr4_1:82823115-82823134 None:intergenic 40.0%
GAAACAAACGAGGTAAGTAG+GGG + chr4_1:82823116-82823135 None:intergenic 40.0%
GACTGACATAGAAACAAACG+AGG + chr4_1:82823126-82823145 None:intergenic 40.0%
TCTATGTATCTGGGCTTCTT+AGG - chr4_1:82821855-82821874 Msa0578260:three_prime_UTR 40.0%
!!! CAGATCTTTTGTTGTTGCAG+TGG - chr4_1:82821941-82821960 Msa0578260:three_prime_UTR 40.0%
AATGGCGATACAGTAAGAGA+TGG - chr4_1:82821987-82822006 Msa0578260:three_prime_UTR 40.0%
CTTGAGTCACGTTATCATCT+TGG + chr4_1:82822088-82822107 None:intergenic 40.0%
AACGTGACTCAAGATTCTTC+TGG - chr4_1:82822095-82822114 Msa0578260:exon 40.0%
! GCTCTTGTTTGCAACAAAGA+GGG - chr4_1:82822311-82822330 Msa0578260:intron 40.0%
CGAAAAGATTGTGAGGCAAA+GGG - chr4_1:82822355-82822374 Msa0578260:intron 40.0%
CTACTACTTCCAGGATTCAA+GGG + chr4_1:82822476-82822495 None:intergenic 40.0%
TCTACTACTTCCAGGATTCA+AGG + chr4_1:82822477-82822496 None:intergenic 40.0%
GAATCCTGGAAGTAGTAGAA+AGG - chr4_1:82822478-82822497 Msa0578260:intron 40.0%
! GAAAAAGCATAGGAACTGAC+CGG + chr4_1:82822521-82822540 None:intergenic 40.0%
! CTTCGGTAGTGAAAAAGCAT+AGG + chr4_1:82822531-82822550 None:intergenic 40.0%
TTTCTCTCGTGGAAAGGATA+AGG + chr4_1:82822994-82823013 None:intergenic 40.0%
TTGCAATTTCTCTCGTGGAA+AGG + chr4_1:82823000-82823019 None:intergenic 40.0%
CTAAGTTGCAATTTCTCTCG+TGG + chr4_1:82823005-82823024 None:intergenic 40.0%
AAACAAACGAGGTAAGTAGG+GGG + chr4_1:82823115-82823134 None:intergenic 40.0%
GAAACAAACGAGGTAAGTAG+GGG + chr4_1:82823116-82823135 None:intergenic 40.0%
GACTGACATAGAAACAAACG+AGG + chr4_1:82823126-82823145 None:intergenic 40.0%
TAACGTATCTTGGTCATGGG+CGG - chr4_1:82821793-82821812 Msa0578260:three_prime_UTR 45.0%
! TACAGTAAGAGATGGTGCCA+TGG - chr4_1:82821995-82822014 Msa0578260:three_prime_UTR 45.0%
TATGCTACCAGCTACAACCA+TGG + chr4_1:82822015-82822034 None:intergenic 45.0%
GTGACTCAAGATTCTTCTGG+AGG - chr4_1:82822098-82822117 Msa0578260:exon 45.0%
GCGTATGATGAATCCAACCT+CGG + chr4_1:82822243-82822262 None:intergenic 45.0%
TAAGCAAGCTCTCACGATCA+AGG - chr4_1:82822264-82822283 Msa0578260:CDS 45.0%
CTCTCACGATCAAGGAAAGA+CGG - chr4_1:82822272-82822291 Msa0578260:CDS 45.0%
AGGAAAGACGGAACTGCTAT+TGG - chr4_1:82822284-82822303 Msa0578260:CDS 45.0%
AAGACGGAACTGCTATTGGA+CGG - chr4_1:82822288-82822307 Msa0578260:CDS 45.0%
! GGCTCTTGTTTGCAACAAAG+AGG - chr4_1:82822310-82822329 Msa0578260:intron 45.0%
! ACAATCTTTTCGCGCTTGTC+AGG + chr4_1:82822347-82822366 None:intergenic 45.0%
ACAAGCGCGAAAAGATTGTG+AGG - chr4_1:82822348-82822367 Msa0578260:intron 45.0%
GCGAAAAGATTGTGAGGCAA+AGG - chr4_1:82822354-82822373 Msa0578260:intron 45.0%
!! AGAAACAAGGGTTGGTTGCA+GGG - chr4_1:82822379-82822398 Msa0578260:intron 45.0%
GCAGGGCAATGATTATGATG+AGG - chr4_1:82822396-82822415 Msa0578260:intron 45.0%
AGCATAATGTGGGTCTCTCA+AGG - chr4_1:82823256-82823275 Msa0578260:CDS 45.0%
GCGTATAGTAGACAGTGTGA+AGG - chr4_1:82823287-82823306 Msa0578260:CDS 45.0%
AGTAGACAGTGTGAAGGAGA+TGG - chr4_1:82823293-82823312 Msa0578260:CDS 45.0%
TAACGTATCTTGGTCATGGG+CGG - chr4_1:82821793-82821812 Msa0578260:three_prime_UTR 45.0%
! TACAGTAAGAGATGGTGCCA+TGG - chr4_1:82821995-82822014 Msa0578260:three_prime_UTR 45.0%
TATGCTACCAGCTACAACCA+TGG + chr4_1:82822015-82822034 None:intergenic 45.0%
GTGACTCAAGATTCTTCTGG+AGG - chr4_1:82822098-82822117 Msa0578260:exon 45.0%
GCGTATGATGAATCCAACCT+CGG + chr4_1:82822243-82822262 None:intergenic 45.0%
TAAGCAAGCTCTCACGATCA+AGG - chr4_1:82822264-82822283 Msa0578260:CDS 45.0%
CTCTCACGATCAAGGAAAGA+CGG - chr4_1:82822272-82822291 Msa0578260:CDS 45.0%
AGGAAAGACGGAACTGCTAT+TGG - chr4_1:82822284-82822303 Msa0578260:CDS 45.0%
AAGACGGAACTGCTATTGGA+CGG - chr4_1:82822288-82822307 Msa0578260:CDS 45.0%
! GGCTCTTGTTTGCAACAAAG+AGG - chr4_1:82822310-82822329 Msa0578260:intron 45.0%
! ACAATCTTTTCGCGCTTGTC+AGG + chr4_1:82822347-82822366 None:intergenic 45.0%
ACAAGCGCGAAAAGATTGTG+AGG - chr4_1:82822348-82822367 Msa0578260:intron 45.0%
GCGAAAAGATTGTGAGGCAA+AGG - chr4_1:82822354-82822373 Msa0578260:intron 45.0%
!! AGAAACAAGGGTTGGTTGCA+GGG - chr4_1:82822379-82822398 Msa0578260:intron 45.0%
GCAGGGCAATGATTATGATG+AGG - chr4_1:82822396-82822415 Msa0578260:intron 45.0%
AGCATAATGTGGGTCTCTCA+AGG - chr4_1:82823256-82823275 Msa0578260:CDS 45.0%
GCGTATAGTAGACAGTGTGA+AGG - chr4_1:82823287-82823306 Msa0578260:CDS 45.0%
AGTAGACAGTGTGAAGGAGA+TGG - chr4_1:82823293-82823312 Msa0578260:CDS 45.0%
TGTAGCTGGTAGCATACCTG+AGG - chr4_1:82822019-82822038 Msa0578260:three_prime_UTR 50.0%
TCGAACTCCTGACCCACATA+GGG + chr4_1:82822179-82822198 None:intergenic 50.0%
TTACAATGCATACGCAGCCG+AGG - chr4_1:82822223-82822242 Msa0578260:CDS 50.0%
AGACGGAACTGCTATTGGAC+GGG - chr4_1:82822289-82822308 Msa0578260:CDS 50.0%
TGAGGCAAAGGGCAGAAACA+AGG - chr4_1:82822366-82822385 Msa0578260:intron 50.0%
GAGGCAAAGGGCAGAAACAA+GGG - chr4_1:82822367-82822386 Msa0578260:intron 50.0%
CAAAGGGCAGAAACAAGGGT+TGG - chr4_1:82822371-82822390 Msa0578260:intron 50.0%
!! CAGAAACAAGGGTTGGTTGC+AGG - chr4_1:82822378-82822397 Msa0578260:intron 50.0%
CACACACATCCCTTGAATCC+TGG - chr4_1:82822464-82822483 Msa0578260:intron 50.0%
CTCTCGTGGAAAGGATAAGG+TGG + chr4_1:82822991-82823010 None:intergenic 50.0%
CCAACTGCTGAGATAGTTCG+CGG + chr4_1:82823170-82823189 None:intergenic 50.0%
TGTAGCTGGTAGCATACCTG+AGG - chr4_1:82822019-82822038 Msa0578260:three_prime_UTR 50.0%
TCGAACTCCTGACCCACATA+GGG + chr4_1:82822179-82822198 None:intergenic 50.0%
TTACAATGCATACGCAGCCG+AGG - chr4_1:82822223-82822242 Msa0578260:CDS 50.0%
AGACGGAACTGCTATTGGAC+GGG - chr4_1:82822289-82822308 Msa0578260:CDS 50.0%
TGAGGCAAAGGGCAGAAACA+AGG - chr4_1:82822366-82822385 Msa0578260:intron 50.0%
GAGGCAAAGGGCAGAAACAA+GGG - chr4_1:82822367-82822386 Msa0578260:intron 50.0%
CAAAGGGCAGAAACAAGGGT+TGG - chr4_1:82822371-82822390 Msa0578260:intron 50.0%
!! CAGAAACAAGGGTTGGTTGC+AGG - chr4_1:82822378-82822397 Msa0578260:intron 50.0%
CACACACATCCCTTGAATCC+TGG - chr4_1:82822464-82822483 Msa0578260:intron 50.0%
CTCTCGTGGAAAGGATAAGG+TGG + chr4_1:82822991-82823010 None:intergenic 50.0%
CCAACTGCTGAGATAGTTCG+CGG + chr4_1:82823170-82823189 None:intergenic 50.0%
!! GATGGTGCCATGGTTGTAGC+TGG - chr4_1:82822005-82822024 Msa0578260:three_prime_UTR 55.0%
! TGGTAGTGTGGGAACTGCAG+CGG + chr4_1:82822138-82822157 None:intergenic 55.0%
!! GCAGAAGCGTCTGGTAGTGT+GGG + chr4_1:82822149-82822168 None:intergenic 55.0%
!! AGCAGAAGCGTCTGGTAGTG+TGG + chr4_1:82822150-82822169 None:intergenic 55.0%
ACTACCAGACGCTTCTGCTG+AGG - chr4_1:82822151-82822170 Msa0578260:CDS 55.0%
TTCTGCTGAGGAGCCCTATG+TGG - chr4_1:82822163-82822182 Msa0578260:CDS 55.0%
TCTGCTGAGGAGCCCTATGT+GGG - chr4_1:82822164-82822183 Msa0578260:CDS 55.0%
CTCGAACTCCTGACCCACAT+AGG + chr4_1:82822180-82822199 None:intergenic 55.0%
TGTGGGTCAGGAGTTCGAGT+CGG - chr4_1:82822181-82822200 Msa0578260:CDS 55.0%
AATGCATACGCAGCCGAGGT+TGG - chr4_1:82822227-82822246 Msa0578260:CDS 55.0%
CCGCGAACTATCTCAGCAGT+TGG - chr4_1:82823167-82823186 Msa0578260:CDS 55.0%
!! GATGGTGCCATGGTTGTAGC+TGG - chr4_1:82822005-82822024 Msa0578260:three_prime_UTR 55.0%
! TGGTAGTGTGGGAACTGCAG+CGG + chr4_1:82822138-82822157 None:intergenic 55.0%
!! GCAGAAGCGTCTGGTAGTGT+GGG + chr4_1:82822149-82822168 None:intergenic 55.0%
!! AGCAGAAGCGTCTGGTAGTG+TGG + chr4_1:82822150-82822169 None:intergenic 55.0%
ACTACCAGACGCTTCTGCTG+AGG - chr4_1:82822151-82822170 Msa0578260:CDS 55.0%
TTCTGCTGAGGAGCCCTATG+TGG - chr4_1:82822163-82822182 Msa0578260:CDS 55.0%
TCTGCTGAGGAGCCCTATGT+GGG - chr4_1:82822164-82822183 Msa0578260:CDS 55.0%
CTCGAACTCCTGACCCACAT+AGG + chr4_1:82822180-82822199 None:intergenic 55.0%
TGTGGGTCAGGAGTTCGAGT+CGG - chr4_1:82822181-82822200 Msa0578260:CDS 55.0%
AATGCATACGCAGCCGAGGT+TGG - chr4_1:82822227-82822246 Msa0578260:CDS 55.0%
CCGCGAACTATCTCAGCAGT+TGG - chr4_1:82823167-82823186 Msa0578260:CDS 55.0%
GGTAGTGTGGGAACTGCAGC+GGG + chr4_1:82822137-82822156 None:intergenic 60.0%
TGAGGAGCCCTATGTGGGTC+AGG - chr4_1:82822169-82822188 Msa0578260:CDS 60.0%
GGTAGTGTGGGAACTGCAGC+GGG + chr4_1:82822137-82822156 None:intergenic 60.0%
TGAGGAGCCCTATGTGGGTC+AGG - chr4_1:82822169-82822188 Msa0578260:CDS 60.0%
!! GGCTCCTCAGCAGAAGCGTC+TGG + chr4_1:82822158-82822177 None:intergenic 65.0%
!! GGCTCCTCAGCAGAAGCGTC+TGG + chr4_1:82822158-82822177 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 82821785 82823676 82821785 ID=Msa0578260;Name=Msa0578260
chr4_1 mRNA 82821785 82823676 82821785 ID=Msa0578260-mRNA-1;Parent=Msa0578260;Name=Msa0578260-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|0.5|0.33|1|0.5|0.66|3|329|290
chr4_1 exon 82821785 82822317 82821785 ID=Msa0578260-mRNA-1:exon:30110;Parent=Msa0578260-mRNA-1
chr4_1 exon 82822941 82823517 82822941 ID=Msa0578260-mRNA-1:exon:30109;Parent=Msa0578260-mRNA-1
chr4_1 exon 82823585 82823676 82823585 ID=Msa0578260-mRNA-1:exon:30108;Parent=Msa0578260-mRNA-1
chr4_1 CDS 82823585 82823676 82823585 ID=Msa0578260-mRNA-1:cds;Parent=Msa0578260-mRNA-1
chr4_1 CDS 82822941 82823517 82822941 ID=Msa0578260-mRNA-1:cds;Parent=Msa0578260-mRNA-1
chr4_1 CDS 82822114 82822317 82822114 ID=Msa0578260-mRNA-1:cds;Parent=Msa0578260-mRNA-1
chr4_1 three_prime_UTR 82821785 82822113 82821785 ID=Msa0578260-mRNA-1:three_prime_utr;Parent=Msa0578260-mRNA-1
Gene Sequence

>Msa0578260

ATGTTTGTTAACGTATCTTGGTCATGGGCGGCACATCTTGATTTTTCGAATATTCCTATATCTGTATCGTTCTATGTATCTGGGCTTCTTAGATCTTTTGTTGTTGCAGTGGATTTTGAGATTGATGACTCTGTTAATGGCGATACAGTAAGAGATGGTGCCATGGTTGTAGCTGGTAGCATACCTGAGGAATACATAGAAGAAGAAAGTTCTGAACCATCTTACAAACAAGACCAAGATGATAACGTGACTCAAGATTCTTCTGGAGGAGTGTTAACAATACCCGCTGCAGTTCCCACACTACCAGACGCTTCTGCTGAGGAGCCCTATGTGGGTCAGGAGTTCGAGTCGGAAGCAGCAGCGCATGCATTTTACAATGCATACGCAGCCGAGGTTGGATTCATCATACGCGTAAGCAAGCTCTCACGATCAAGGAAAGACGGAACTGCTATTGGACGGGCTCTTGTTTGCAACAAAGAGGGTTACAGAATGCCTGACAAGCGCGAAAAGATTGTGAGGCAAAGGGCAGAAACAAGGGTTGGTTGCAGGGCAATGATTATGATGAGGAAAATTAATTCTGGTAAATGGGTTATTACGAAGTTTGTAAAGGAGCACACACATCCCTTGAATCCTGGAAGTAGTAGAAAGGATATGTTTGAAATTTATCCGTCTCAGAACGAACATGATAAAATCCGCGAACTATCTCAGCAGTTGGCAATAGAGAAAAAGCGATCTGTGACATATAAAAGACAACTTGAAGTGATATTTGATTATATCGAGGAGCATAATGTGGGTCTCTCAAGGAAAATGCAGCGTATAGTAGACAGTGTGAAGGAGATGGAACCTAAAGAAGAAGAAGAACATAATCATTGA
Protein sequence

>Msa0578260

MFVNVSWSWAAHLDFSNIPISVSFYVSGLLRSFVVAVDFEIDDSVNGDTVRDGAMVVAGSIPEEYIEEESSEPSYKQDQDDNVTQDSSGGVLTIPAAVPTLPDASAEEPYVGQEFESEAAAHAFYNAYAAEVGFIIRVSKLSRSRKDGTAIGRALVCNKEGYRMPDKREKIVRQRAETRVGCRAMIMMRKINSGKWVITKFVKEHTHPLNPGSSRKDMFEIYPSQNEHDKIRELSQQLAIEKKRSVTYKRQLEVIFDYIEEHNVGLSRKMQRIVDSVKEMEPKEEEEHNH*