Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452170 | sp|Q9SZL8|FRS5_ARATH | 54.630 | 108 | 45 | 2 | 137 | 240 | 72 | 179 | 6.42e-23 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452170 | A0A072UKW2 | 94.961 | 258 | 12 | 1 | 63 | 319 | 40 | 297 | 1.30e-169 | 482 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0042980 | Msa1452170 | -0.802575 | 5.256685e-49 | -8.615850e-47 |
| Msa0137710 | Msa1452170 | -0.817660 | 2.939367e-52 | -8.615850e-47 |
| Msa0268090 | Msa1452170 | 0.818883 | 1.553870e-52 | -8.615850e-47 |
| Msa0279210 | Msa1452170 | -0.809976 | 1.450046e-50 | -8.615850e-47 |
| Msa0318970 | Msa1452170 | -0.810234 | 1.275701e-50 | -8.615850e-47 |
| Msa0385490 | Msa1452170 | 0.805846 | 1.096036e-49 | -8.615850e-47 |
| Msa0412840 | Msa1452170 | 0.809925 | 1.486712e-50 | -8.615850e-47 |
| Msa0457510 | Msa1452170 | 0.808794 | 2.599285e-50 | -8.615850e-47 |
| Msa0468130 | Msa1452170 | 0.801227 | 9.946646e-49 | -8.615850e-47 |
| Msa0546570 | Msa1452170 | 0.800550 | 1.367502e-48 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452170 | MtrunA17_Chr4g0033611 | 95.686 | 255 | 10 | 1 | 66 | 319 | 3 | 257 | 2.99e-173 | 480 |
| Msa1452170 | MtrunA17_Chr2g0329421 | 70.388 | 206 | 56 | 4 | 112 | 315 | 1 | 203 | 1.68e-99 | 291 |
| Msa1452170 | MtrunA17_Chr3g0078041 | 43.684 | 190 | 97 | 3 | 132 | 317 | 44 | 227 | 5.16e-43 | 147 |
| Msa1452170 | MtrunA17_Chr2g0329361 | 36.923 | 195 | 105 | 6 | 130 | 310 | 53 | 243 | 4.95e-34 | 125 |
| Msa1452170 | MtrunA17_Chr7g0233911 | 36.559 | 186 | 105 | 4 | 130 | 310 | 26 | 203 | 1.64e-32 | 120 |
| Msa1452170 | MtrunA17_Chr5g0448761 | 50.862 | 116 | 52 | 3 | 130 | 240 | 52 | 167 | 5.26e-22 | 96.7 |
| Msa1452170 | MtrunA17_Chr2g0287061 | 42.202 | 109 | 51 | 1 | 137 | 245 | 182 | 278 | 3.19e-21 | 94.4 |
| Msa1452170 | MtrunA17_Chr2g0287061 | 37.255 | 102 | 52 | 1 | 137 | 238 | 28 | 117 | 5.25e-16 | 79.0 |
| Msa1452170 | MtrunA17_Chr1g0203721 | 36.943 | 157 | 87 | 5 | 109 | 256 | 27 | 180 | 8.70e-20 | 90.1 |
| Msa1452170 | MtrunA17_Chr3g0082121 | 44.860 | 107 | 53 | 3 | 138 | 240 | 43 | 147 | 1.20e-19 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452170 | AT3G59470.2 | 54.545 | 264 | 107 | 5 | 66 | 319 | 1 | 261 | 4.16e-94 | 280 |
| Msa1452170 | AT3G59470.3 | 63.084 | 214 | 69 | 4 | 107 | 319 | 45 | 249 | 1.98e-93 | 278 |
| Msa1452170 | AT3G59470.1 | 63.084 | 214 | 69 | 4 | 107 | 319 | 45 | 249 | 1.98e-93 | 278 |
| Msa1452170 | AT3G07500.1 | 42.938 | 177 | 98 | 3 | 137 | 310 | 32 | 208 | 4.66e-43 | 148 |
| Msa1452170 | AT2G43280.1 | 38.571 | 210 | 105 | 5 | 106 | 310 | 2 | 192 | 1.26e-36 | 130 |
| Msa1452170 | AT4G12850.1 | 36.667 | 180 | 106 | 3 | 132 | 310 | 5 | 177 | 8.48e-32 | 117 |
| Msa1452170 | AT2G43280.2 | 39.759 | 166 | 88 | 4 | 153 | 313 | 2 | 160 | 2.21e-30 | 113 |
| Msa1452170 | AT4G12850.2 | 41.463 | 123 | 69 | 2 | 132 | 253 | 5 | 125 | 1.92e-24 | 97.1 |
| Msa1452170 | AT4G38180.1 | 54.630 | 108 | 45 | 2 | 137 | 240 | 72 | 179 | 6.53e-24 | 102 |
| Msa1452170 | AT4G12850.4 | 42.056 | 107 | 60 | 1 | 132 | 238 | 5 | 109 | 3.02e-23 | 93.6 |
Find 86 sgRNAs with CRISPR-Local
Find 261 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATGTTGCAAAATCAGCTA+TGG | 0.513100 | tig0033702:+1684 | None:intergenic |
| AACGTGACTCAAGATTCTTC+TGG | 0.391633 | tig0033702:-3250 | Msa1452170:CDS |
| AAGACGGAACTGCTATTGGA+CGG | 0.612304 | tig0033702:-3057 | Msa1452170:CDS |
| AATGCATACGCAGCCGAGGT+TGG | 0.541461 | tig0033702:-3118 | Msa1452170:CDS |
| AATGGCGATACAGTAAGAGA+TGG | 0.645018 | tig0033702:-3358 | Msa1452170:CDS |
| ACAAGCGCGAAAAGATTGTG+AGG | 0.665448 | tig0033702:-2997 | Msa1452170:CDS |
| ACTACCAGACGCTTCTGCTG+AGG | 0.646967 | tig0033702:-3194 | Msa1452170:CDS |
| AGAAACAAGGGTTGGTTGCA+GGG | 0.508414 | tig0033702:-2966 | Msa1452170:CDS |
| AGAAGAACATAATCATTGAT+AGG | 0.432866 | tig0033702:-2016 | Msa1452170:exon |
| AGACGGAACTGCTATTGGAC+GGG | 0.589838 | tig0033702:-3056 | Msa1452170:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGATTTGAATTTGAATGCAG+TGG | - | tig0033702:1741-1760 | Msa1452170:three_prime_UTR | 30.0% | |
| CAAAGAAAGATATTTAGGTG+CGG | + | tig0033702:2042-2061 | None:intergenic | 30.0% | |
| AAACTCACTGAAAAAATCGA+TGG | + | tig0033702:2139-2158 | None:intergenic | 30.0% | |
| TCAAAATTGAAGCTTGATGT+TGG | - | tig0033702:2327-2346 | Msa1452170:intron | 30.0% | |
| TAATCAACTCAACTATGTGT+CGG | + | tig0033702:2666-2685 | None:intergenic | 30.0% | |
| ACAAATTATGGTCATGTTTG+AGG | - | tig0033702:2818-2837 | Msa1452170:intron | 30.0% | |
| TACTAAAACTCCACATTCTT+TGG | + | tig0033702:2944-2963 | None:intergenic | 30.0% | |
| GGATAGTATTTGACAATCAT+TGG | - | tig0033702:3368-3387 | Msa1452170:CDS | 30.0% | |
| GATATCGATACTTTCCAATT+TGG | + | tig0033702:4072-4091 | None:intergenic | 30.0% | |
| ATTGAAGATGCATATTGCAT+CGG | + | tig0033702:4165-4184 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0033702 | gene | 1691 | 6286 | 1691 | ID=Msa1452170;Name=Msa1452170 |
| tig0033702 | mRNA | 1691 | 6286 | 1691 | ID=Msa1452170-mRNA-1;Parent=Msa1452170;Name=Msa1452170-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|0.5|0.66|0.66|1|1|3|329|319 |
| tig0033702 | exon | 1691 | 2223 | 1691 | ID=Msa1452170-mRNA-1:exon:18464;Parent=Msa1452170-mRNA-1 |
| tig0033702 | exon | 2847 | 3406 | 2847 | ID=Msa1452170-mRNA-1:exon:18463;Parent=Msa1452170-mRNA-1 |
| tig0033702 | exon | 6091 | 6286 | 6091 | ID=Msa1452170-mRNA-1:exon:18462;Parent=Msa1452170-mRNA-1 |
| tig0033702 | CDS | 6091 | 6286 | 6091 | ID=Msa1452170-mRNA-1:cds;Parent=Msa1452170-mRNA-1 |
| tig0033702 | CDS | 2847 | 3406 | 2847 | ID=Msa1452170-mRNA-1:cds;Parent=Msa1452170-mRNA-1 |
| tig0033702 | CDS | 2020 | 2223 | 2020 | ID=Msa1452170-mRNA-1:cds;Parent=Msa1452170-mRNA-1 |
| tig0033702 | three_prime_UTR | 1691 | 2019 | 1691 | ID=Msa1452170-mRNA-1:three_prime_utr;Parent=Msa1452170-mRNA-1 |
| Gene Sequence |
>Msa1452170 ATGAGAAAGAACCTGGGAGGGACCGAAAACGAGGTAGCAGCATACGAAGATGATTTGAATTTGAATGCAGTGGAAGCTCTACGAATAGTGCGGAACAACGTAGTTGCCATGGTAAGAGATGAAGAAAAACTTGAACAGTGTTGTGACTATGTTTGTGTTGTTGTGTTGTTCTGGTGCACCACCCGTATTGGATCGGTGGATTTTGAGATTGATGACTCTGTTAATGGCGATACAGTAAGAGATGGTGCCATGGTTGTAGCTGGTAGCATACCTGAGGAATACATAGAAGAAGAAAGTTCTGAACCATCTTACAAACAAGACCAAGATGATAACGTGACTCAAGATTCTTCTGGAGGAGTGTTAACAATACCCGCTGCAGTTCCCACACTACCAGACGCTTCTGCTGAGGAGCCCTATGTGGGTCAGGAGTTCGAGTCGGAAGCAGCAGCGCATGCATTTTACAATGCATACGCAGCCGAGGTTGGATTCATCATACGCGTAAGCAAGCTCTCACGATCAAGGAAAGACGGAACTGCTATTGGACGGGCTCTTGTTTGCAACAAAGAGGGTTACAGAATGCCTGACAAGCGCGAAAAGATTGTGAGGCAAAGGGCAGAAACAAGGGTTGGTTGCAGGGCAATGATTATGATGAGGAAAATTAATTCTGGTAAATGGGTTATTACGAAGTTTGTAAAGGAGCACACACATCCCTTGAATCCTGGAAGTAGTAGAAAGGATATGTTTGAAATTTATCCGTCTCAGAACGAACATGATAAAATCCGCGAACTATCTCAGCAGTTGGCAATAGAGAAAAAGCGATCTGTGACATATAAAAGACAACTTGAAGTGATATTTGATTATATCGAGGAGCATAATGTGGGTCTCTCAAGGAAAATGCAGCGTATAGTAGACAGTGTGAAGGAGATGGAACCTAAAGAAGAAGAAGAACATAATCATTGA |
| Protein sequence |
>Msa1452170 MRKNLGGTENEVAAYEDDLNLNAVEALRIVRNNVVAMVRDEEKLEQCCDYVCVVVLFWCTTRIGSVDFEIDDSVNGDTVRDGAMVVAGSIPEEYIEEESSEPSYKQDQDDNVTQDSSGGVLTIPAAVPTLPDASAEEPYVGQEFESEAAAHAFYNAYAAEVGFIIRVSKLSRSRKDGTAIGRALVCNKEGYRMPDKREKIVRQRAETRVGCRAMIMMRKINSGKWVITKFVKEHTHPLNPGSSRKDMFEIYPSQNEHDKIRELSQQLAIEKKRSVTYKRQLEVIFDYIEEHNVGLSRKMQRIVDSVKEMEPKEEEEHNH* |
