AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0610480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610480 MtrunA17_Chr4g0043431 94.750 400 19 1 1 398 1 400 0.0 759
Msa0610480 MtrunA17_Chr2g0291841 67.656 337 104 3 65 398 28 362 5.64e-166 469
Msa0610480 MtrunA17_Chr7g0230891 53.728 389 174 3 14 398 14 400 2.20e-152 436
Msa0610480 MtrunA17_Chr2g0303941 52.551 392 181 5 8 398 4 391 5.56e-138 399
Msa0610480 MtrunA17_Chr1g0195221 41.207 381 210 9 28 398 30 406 8.22e-91 279
Msa0610480 MtrunA17_Chr8g0337351 74.850 167 42 0 165 331 1 167 3.70e-88 264
Msa0610480 MtrunA17_Chr1g0173451 43.085 376 203 8 25 397 68 435 1.56e-87 272
Msa0610480 MtrunA17_Chr8g0348981 38.596 399 230 9 10 398 5 398 9.88e-85 263
Msa0610480 MtrunA17_Chr2g0332801 41.129 372 210 8 34 398 60 429 7.01e-84 263
Msa0610480 MtrunA17_Chr2g0307831 38.046 389 218 7 8 383 1 379 5.18e-83 259
Msa0610480 MtrunA17_Chr2g0307821 38.046 389 218 7 8 383 1 379 5.18e-83 259
Msa0610480 MtrunA17_Chr8g0365901 40.318 377 205 11 34 398 66 434 1.13e-82 260
Msa0610480 MtrunA17_Chr4g0067631 37.634 372 221 7 34 397 53 421 2.52e-82 258
Msa0610480 MtrunA17_Chr6g0449961 40.318 377 212 10 27 398 39 407 3.37e-80 253
Msa0610480 MtrunA17_Chr2g0307811 38.243 387 220 8 8 383 1 379 6.55e-80 251
Msa0610480 MtrunA17_Chr5g0418411 36.476 403 241 9 4 398 1 396 9.58e-77 243
Msa0610480 MtrunA17_Chr6g0461181 33.014 418 227 9 27 398 24 434 2.10e-74 238
Msa0610480 MtrunA17_Chr4g0020771 40.407 344 192 9 35 370 76 414 7.69e-74 237
Msa0610480 MtrunA17_Chr3g0121891 37.366 372 217 7 8 371 4 367 1.61e-73 234
Msa0610480 MtrunA17_Chr3g0089021 41.304 368 195 10 23 374 51 413 3.22e-73 235
Msa0610480 MtrunA17_Chr4g0043661 35.236 403 229 13 11 398 9 394 7.15e-71 228
Msa0610480 MtrunA17_Chr3g0121881 34.937 395 242 10 1 387 1 388 4.20e-70 226
Msa0610480 MtrunA17_Chr8g0365891 34.815 405 226 9 29 398 103 504 4.40e-69 226
Msa0610480 MtrunA17_Chr5g0413411 36.927 371 201 7 30 370 118 485 6.37e-69 226
Msa0610480 MtrunA17_Chr1g0194231 36.504 389 226 12 28 398 76 461 1.43e-68 224
Msa0610480 MtrunA17_Chr2g0295451 39.262 298 174 4 25 316 45 341 2.02e-68 221
Msa0610480 MtrunA17_Chr2g0332971 36.816 402 234 10 10 398 8 402 9.36e-68 220
Msa0610480 MtrunA17_Chr4g0052621 37.629 388 221 14 28 398 60 443 1.29e-67 221
Msa0610480 MtrunA17_Chr7g0270361 36.951 387 224 12 29 398 63 446 1.76e-67 221
Msa0610480 MtrunA17_Chr1g0150291 34.321 405 247 12 2 398 17 410 1.42e-66 217
Msa0610480 MtrunA17_Chr1g0150301 34.837 399 242 11 4 395 1 388 3.01e-66 216
Msa0610480 MtrunA17_Chr5g0413141 34.211 380 229 12 32 396 58 431 2.84e-64 212
Msa0610480 MtrunA17_Chr4g0018151 36.181 398 234 10 10 398 54 440 2.07e-63 209
Msa0610480 MtrunA17_Chr8g0337581 34.406 404 241 12 4 398 1 389 1.12e-62 206
Msa0610480 MtrunA17_Chr5g0447901 37.539 317 185 9 28 337 20 330 1.46e-62 204
Msa0610480 MtrunA17_Chr7g0228411 33.000 400 224 12 7 370 9 400 2.59e-62 206
Msa0610480 MtrunA17_Chr8g0337571 34.819 359 214 8 4 356 1 345 6.88e-62 204
Msa0610480 MtrunA17_Chr3g0093011 39.844 256 148 4 148 398 7 261 1.58e-57 189
Msa0610480 MtrunA17_Chr7g0257721 41.852 270 151 5 21 285 63 331 4.85e-57 190
Msa0610480 MtrunA17_Chr7g0257701 40.557 323 147 9 33 317 65 380 1.57e-56 191
Msa0610480 MtrunA17_Chr5g0425811 39.209 278 158 6 50 323 2 272 1.45e-54 181
Msa0610480 MtrunA17_Chr3g0100091 36.774 310 179 6 95 398 48 346 1.10e-53 182
Msa0610480 MtrunA17_Chr2g0293681 36.418 335 198 7 20 342 54 385 2.20e-52 180
Msa0610480 MtrunA17_Chr8g0337521 37.722 281 155 6 8 284 9 273 3.08e-50 171
Msa0610480 MtrunA17_Chr8g0337341 51.316 152 72 2 3 153 5 155 6.64e-48 160
Msa0610480 MtrunA17_Chr8g0336481 37.079 267 154 6 93 356 14 269 5.90e-47 161
Msa0610480 MtrunA17_Chr1g0149241 30.946 391 221 12 11 394 9 357 1.18e-44 158
Msa0610480 MtrunA17_Chr8g0337601 39.024 246 141 5 155 398 2 240 1.25e-43 152
Msa0610480 MtrunA17_Chr8g0337511 36.777 242 147 3 154 395 4 239 2.85e-40 143
Msa0610480 MtrunA17_Chr7g0273191 43.796 137 75 1 134 268 2 138 2.38e-31 116
Msa0610480 MtrunA17_Chr8g0337551 44.531 128 71 0 157 284 7 134 3.38e-29 110
Msa0610480 MtrunA17_Chr4g0070281 27.764 407 238 18 32 397 74 465 3.26e-23 101
Msa0610480 MtrunA17_Chr8g0337541 34.694 147 90 4 4 145 1 146 5.58e-23 94.4
Msa0610480 MtrunA17_Chr3g0093021 36.076 158 90 6 11 163 5 156 5.60e-21 89.4
Msa0610480 MtrunA17_Chr7g0257711 44.860 107 57 2 291 395 6 112 7.69e-21 87.4
Msa0610480 MtrunA17_Chr3g0095221 27.415 383 223 15 27 370 41 407 1.71e-19 90.1
Msa0610480 MtrunA17_Chr7g0270821 26.034 411 232 15 27 382 37 430 1.98e-19 89.7
Msa0610480 MtrunA17_Chr7g0255311 28.278 389 232 17 18 370 48 425 2.71e-19 89.7
Msa0610480 MtrunA17_Chr2g0301091 25.296 423 264 16 14 397 37 446 5.71e-19 88.6
Msa0610480 MtrunA17_Chr8g0387391 27.654 405 236 18 34 397 45 433 6.64e-19 88.2
Msa0610480 MtrunA17_Chr8g0348491 25.537 419 265 14 14 397 38 444 1.81e-18 87.0
Msa0610480 MtrunA17_Chr5g0442221 25.799 407 257 14 27 397 38 435 1.88e-18 86.7
Msa0610480 MtrunA17_Chr1g0202651 26.683 401 239 17 37 397 59 444 1.99e-18 87.0
Msa0610480 MtrunA17_Chr1g0202641 26.683 401 239 18 37 397 59 444 3.57e-18 87.0
Msa0610480 MtrunA17_Chr1g0202641 25.935 401 242 18 37 397 559 944 1.42e-16 82.0
Msa0610480 MtrunA17_Chr7g0248451 25.776 419 264 15 14 397 38 444 1.41e-17 84.3
Msa0610480 MtrunA17_Chr1g0193961 29.316 307 173 12 35 307 45 341 2.53e-17 83.6
Msa0610480 MtrunA17_Chr2g0330221 29.747 158 108 3 47 202 37 193 5.95e-17 79.0
Msa0610480 MtrunA17_Chr7g0273181 38.384 99 58 2 303 398 1 99 9.17e-17 76.3
Msa0610480 MtrunA17_Chr1g0202621 26.147 436 261 21 7 397 12 431 1.50e-15 78.2
Msa0610480 MtrunA17_Chr3g0130251 25.466 322 175 12 34 310 77 378 5.86e-15 76.3
Msa0610480 MtrunA17_Chr4g0067811 23.958 384 242 16 25 371 32 402 6.19e-15 76.3
Msa0610480 MtrunA17_Chr8g0336471 35.294 85 53 2 4 86 1 85 6.03e-12 61.6
Msa0610480 MtrunA17_Chr2g0295241 24.586 362 214 12 35 368 39 369 3.02e-11 64.7
Msa0610480 MtrunA17_Chr8g0337561 34.545 110 64 3 291 398 11 114 7.21e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0610480 AT3G59850.2 65.946 370 124 2 30 398 64 432 0.0 516
Msa0610480 AT3G59850.1 65.946 370 124 2 30 398 20 388 0.0 514
Msa0610480 AT2G43870.1 62.663 383 139 4 17 398 5 384 1.79e-179 504
Msa0610480 AT1G05660.1 52.455 387 179 4 13 398 10 392 3.57e-148 426
Msa0610480 AT1G05650.1 52.196 387 180 4 13 398 10 392 8.25e-147 422
Msa0610480 AT2G43890.1 50.000 394 192 3 5 398 4 392 5.72e-143 412
Msa0610480 AT2G43860.1 55.145 379 168 2 21 398 21 398 8.48e-141 407
Msa0610480 AT1G65570.1 49.114 395 197 4 7 398 4 397 1.78e-140 406
Msa0610480 AT2G43880.1 50.541 370 181 2 29 398 27 394 4.59e-133 387
Msa0610480 AT2G43880.2 52.013 298 141 2 29 326 27 322 3.83e-106 316
Msa0610480 AT1G17150.1 41.960 398 214 8 8 398 14 401 6.50e-93 285
Msa0610480 AT3G07970.1 41.114 377 214 6 24 394 62 436 7.21e-92 283
Msa0610480 AT2G40310.1 39.851 404 226 11 8 398 4 403 4.75e-90 278
Msa0610480 AT4G13760.1 41.489 376 207 9 33 398 31 403 2.37e-89 276
Msa0610480 AT1G43080.1 40.769 390 213 10 24 398 17 403 3.50e-89 275
Msa0610480 AT2G15450.1 40.704 398 212 12 19 398 12 403 2.56e-87 271
Msa0610480 AT1G02790.1 38.250 400 224 8 1 386 8 398 2.62e-87 271
Msa0610480 AT3G57510.1 41.935 372 203 7 30 395 65 429 6.76e-87 270
Msa0610480 AT2G15470.1 40.201 398 214 12 19 398 12 403 9.34e-87 269
Msa0610480 AT1G70500.1 39.637 386 222 9 21 398 54 436 3.55e-86 270
Msa0610480 AT2G15460.1 40.506 395 211 12 19 395 12 400 7.45e-86 267
Msa0610480 AT1G43100.1 40.838 382 208 10 24 390 17 395 9.39e-86 268
Msa0610480 AT1G43090.1 39.695 393 217 10 8 390 13 395 1.20e-85 268
Msa0610480 AT2G33160.1 39.500 400 224 10 14 397 5 402 1.54e-85 273
Msa0610480 AT1G78400.1 40.054 372 216 6 32 398 34 403 3.46e-85 265
Msa0610480 AT4G18180.1 37.532 389 225 6 27 397 33 421 4.70e-84 263
Msa0610480 AT2G26620.1 40.909 374 210 8 33 398 31 401 1.30e-83 261
Msa0610480 AT1G80170.1 39.005 382 223 8 25 398 45 424 8.11e-83 260
Msa0610480 AT3G07830.1 37.405 393 231 10 10 397 5 387 1.18e-81 256
Msa0610480 AT2G41850.1 41.176 374 201 9 31 395 66 429 2.18e-81 256
Msa0610480 AT1G23460.2 40.933 386 213 12 23 398 32 412 8.14e-81 255
Msa0610480 AT3G07840.1 38.133 375 218 9 30 397 20 387 1.06e-80 253
Msa0610480 AT1G23460.1 40.933 386 213 12 23 398 56 436 1.62e-80 255
Msa0610480 AT3G07820.1 38.936 357 210 7 46 397 33 386 5.89e-80 251
Msa0610480 AT3G14040.1 39.314 379 218 6 26 397 64 437 2.20e-79 251
Msa0610480 AT5G48140.1 37.270 381 224 9 24 397 14 386 9.73e-79 248
Msa0610480 AT3G15720.1 38.760 387 218 7 20 398 14 389 7.84e-77 245
Msa0610480 AT3G15720.3 38.760 387 218 7 20 398 13 388 7.88e-77 245
Msa0610480 AT3G07850.1 38.503 374 218 6 31 397 68 436 4.30e-76 243
Msa0610480 AT3G15720.2 38.760 387 215 8 20 398 13 385 2.14e-75 241
Msa0610480 AT5G14650.1 37.795 381 214 9 8 370 11 386 2.47e-75 241
Msa0610480 AT4G35670.1 37.903 372 218 9 33 398 24 388 3.44e-74 236
Msa0610480 AT1G80140.1 41.691 343 182 9 66 398 2 336 4.36e-74 234
Msa0610480 AT1G56710.1 38.425 419 227 13 7 398 13 427 1.35e-72 233
Msa0610480 AT1G60590.1 37.010 408 213 11 30 398 120 522 4.94e-72 235
Msa0610480 AT5G27530.1 33.911 404 245 8 2 398 21 409 2.76e-71 231
Msa0610480 AT1G10640.1 35.980 403 221 10 30 398 116 515 3.80e-71 233
Msa0610480 AT5G17200.1 37.337 383 205 8 35 391 41 414 6.06e-71 229
Msa0610480 AT5G44840.1 34.656 378 223 10 33 398 24 389 2.59e-69 224
Msa0610480 AT1G02460.1 38.501 387 214 14 33 398 89 472 8.07e-69 225
Msa0610480 AT3G15720.4 39.275 331 186 5 75 398 3 325 3.04e-66 216
Msa0610480 AT4G01890.1 37.535 357 200 12 33 370 65 417 1.19e-64 214
Msa0610480 AT3G26610.1 35.171 381 225 10 33 396 67 442 1.54e-64 214
Msa0610480 AT1G48100.1 35.602 382 227 11 33 398 80 458 1.28e-63 211
Msa0610480 AT4G32380.1 37.349 332 193 7 47 370 41 365 2.22e-61 205
Msa0610480 AT5G44830.1 34.451 328 195 6 75 398 19 330 1.94e-58 194
Msa0610480 AT5G39910.1 32.530 415 203 11 6 398 9 368 4.37e-58 194
Msa0610480 AT4G32375.1 41.538 260 144 5 114 371 13 266 1.71e-57 194
Msa0610480 AT4G35670.2 37.979 287 169 6 114 398 2 281 3.88e-56 186
Msa0610480 AT4G32370.1 39.799 299 158 10 47 332 49 338 2.22e-55 186
Msa0610480 AT5G14650.2 37.500 296 165 6 8 285 11 304 6.18e-55 184
Msa0610480 AT1G80170.2 40.075 267 153 6 25 285 45 310 6.56e-55 184
Msa0610480 AT4G32380.2 32.827 329 171 5 47 370 41 324 4.15e-47 166
Msa0610480 AT3G48950.1 27.419 434 256 17 10 397 15 435 1.07e-23 102
Msa0610480 AT1G19170.1 28.013 307 193 11 110 397 181 478 9.79e-23 100
Msa0610480 AT4G23820.1 27.970 404 234 17 34 397 42 428 4.37e-21 95.1
Msa0610480 AT3G61490.2 25.450 444 271 16 4 397 1 434 7.38e-21 94.7
Msa0610480 AT3G61490.3 25.450 444 271 16 4 397 1 434 7.38e-21 94.7
Msa0610480 AT3G61490.1 25.450 444 271 16 4 397 1 434 7.38e-21 94.7
Msa0610480 AT3G62110.1 27.147 361 220 13 16 342 26 377 8.52e-21 94.4
Msa0610480 AT3G62110.2 27.147 361 220 13 16 342 26 377 8.52e-21 94.4
Msa0610480 AT3G61490.5 25.450 444 271 16 4 397 16 449 9.26e-21 94.4
Msa0610480 AT3G61490.4 25.450 444 271 16 4 397 114 547 2.30e-20 93.6
Msa0610480 AT3G42950.1 26.139 417 242 15 27 397 60 456 5.58e-20 92.0
Msa0610480 AT2G23900.1 26.434 401 241 15 37 397 60 446 1.94e-19 90.5
Msa0610480 AT5G49215.1 26.055 403 246 15 35 398 45 434 5.92e-19 88.6
Msa0610480 AT5G49215.2 26.055 403 246 15 35 398 43 432 6.43e-19 88.6
Msa0610480 AT5G41870.1 26.303 403 256 12 27 397 39 432 1.02e-18 88.2
Msa0610480 AT3G16850.1 25.116 430 270 13 7 397 10 426 2.03e-18 87.0
Msa0610480 AT4G33440.1 27.986 293 154 13 128 381 185 459 3.33e-16 80.5
Msa0610480 AT4G23500.1 24.638 414 259 19 22 397 59 457 6.63e-16 79.7
Msa0610480 AT3G06770.2 25.432 405 244 18 34 397 44 431 1.30e-12 69.3
Msa0610480 AT3G06770.5 25.432 405 244 18 34 397 45 432 1.40e-12 69.3

Find 109 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCATCACCGGTTCGAATTT+TGG 0.125064 4_2:-58230102 None:intergenic
CCGGGGCAGCCTTCGTTGTT+TGG 0.192728 4_2:-58230535 None:intergenic
TTGTTACTTAAATTCCTTTA+CGG 0.225568 4_2:+58228875 Msa0610480:CDS
CGTTGTTTGGGCAATAATTC+TGG 0.240499 4_2:-58230522 None:intergenic
GTCGGTGTTTGGGCTGTTTC+CGG 0.278257 4_2:-58230036 None:intergenic
CTTGGAACTGCAACATTTAG+TGG 0.283715 4_2:+58229050 Msa0610480:CDS
GGTCAATTACAATTGGGTTT+TGG 0.298931 4_2:-58230501 None:intergenic
CGGGGCAGCCTTCGTTGTTT+GGG 0.304377 4_2:-58230534 None:intergenic
ACGTGGATGCCGTCGGTGTT+TGG 0.336638 4_2:-58230047 None:intergenic
GCTATTTCCATAACCATTGA+TGG 0.348650 4_2:+58229089 Msa0610480:CDS
TAATTCTGGTCAATTACAAT+TGG 0.351868 4_2:-58230508 None:intergenic
GTGGACCGGGACATGGAATA+AGG 0.352685 4_2:+58230177 Msa0610480:CDS
ACATTCACAGGAACTCAAAA+TGG 0.357786 4_2:+58230397 Msa0610480:CDS
ATCCGCGGCGGTGTGCTTGA+TGG 0.379802 4_2:+58229194 Msa0610480:CDS
CAAAGTGCCATCAATGGTTA+TGG 0.385028 4_2:-58229096 None:intergenic
ATTTCTAAATGTATGGTCTA+AGG 0.389157 4_2:+58228974 Msa0610480:CDS
CCACAAGGGAAATTCTTACT+TGG 0.390390 4_2:+58229032 Msa0610480:CDS
CAGATGTTCCATGAATGTTT+TGG 0.392328 4_2:-58230784 None:intergenic
TGGTGACTTGGGAGGACATT+TGG 0.394523 4_2:-58230070 None:intergenic
CGTGGATGCCGTCGGTGTTT+GGG 0.397704 4_2:-58230046 None:intergenic
TACATTTAGAAATGCCTTTG+TGG 0.399821 4_2:-58228964 None:intergenic
CCATGCTGGTGGAGCTGCAT+TGG 0.401798 4_2:+58230919 Msa0610480:CDS
TGGTGTTAGAATAAAGTCTT+GGG 0.413293 4_2:+58230417 Msa0610480:CDS
GACAAAAGTAATTGCCCAAC+TGG 0.419215 4_2:+58229251 Msa0610480:CDS
ACATACAATGTAATCAACTA+TGG 0.426913 4_2:+58228915 Msa0610480:CDS
ACGTAAATTGCCGCCGGATA+AGG 0.427718 4_2:-58229008 None:intergenic
AATTCTGGTCAATTACAATT+GGG 0.433868 4_2:-58230507 None:intergenic
TCATCTAATTACCGTGTAAC+CGG 0.434207 4_2:+58229125 Msa0610480:CDS
GGAGTTGAGGATGGTGACTT+GGG 0.435300 4_2:-58230081 None:intergenic
GTTAAAACGGTTACATTCAC+AGG 0.436737 4_2:+58230385 Msa0610480:CDS
CAACGAAGGCTGCCCCGGTC+AGG 0.438018 4_2:+58230540 Msa0610480:CDS
GGTGATGATTGTATCTCAAT+TGG 0.439090 4_2:+58230116 Msa0610480:CDS
CAAAGGCATTTCTAAATGTA+TGG 0.440684 4_2:+58228967 Msa0610480:CDS
GTAACATACCAAAACATTCA+TGG 0.441891 4_2:+58230776 Msa0610480:CDS
CATTAACCATGATTGCATCT+TGG 0.446209 4_2:-58230477 None:intergenic
TACATGATGCTTCAGCCACT+TGG 0.449945 4_2:-58230895 None:intergenic
TGTTGCAGTATTGGAAGCTT+GGG 0.450951 4_2:+58230325 Msa0610480:intron
TTGTTGCAGTATTGGAAGCT+TGG 0.451276 4_2:+58230324 Msa0610480:intron
TGGAGTTGAGGATGGTGACT+TGG 0.452456 4_2:-58230082 None:intergenic
ATGGTGTTAGAATAAAGTCT+TGG 0.456513 4_2:+58230416 Msa0610480:CDS
AAATTATTAAATCAGGCCTC+TGG 0.458353 4_2:+58230737 Msa0610480:intron
CATAACCATTGATGGCACTT+TGG 0.461412 4_2:+58229097 Msa0610480:CDS
TGAAGTTTCAGCATGTTGAC+GGG 0.462893 4_2:+58229165 Msa0610480:CDS
CATGCTGGTGGAGCTGCATT+GGG 0.465376 4_2:+58230920 Msa0610480:CDS
GCCCTGGTACAAGTAATTTG+TGG 0.471238 4_2:+58230138 Msa0610480:CDS
CCAAACAACGAAGGCTGCCC+CGG 0.472065 4_2:+58230535 Msa0610480:CDS
TTGAAGTTTCAGCATGTTGA+CGG 0.472330 4_2:+58229164 Msa0610480:CDS
GTGATTATGATTAAGAGAAA+AGG 0.478653 4_2:-58228846 None:intergenic
GGCATGTTCCTCGCCTTATC+CGG 0.479418 4_2:+58228995 Msa0610480:CDS
TGAAAACATTGCATGTGGAC+CGG 0.479846 4_2:+58230163 Msa0610480:CDS
ACTATTCATCGATGTCAAAC+CGG 0.483988 4_2:-58229946 None:intergenic
GGTGTCTATAAGCAACTCTC+TGG 0.484363 4_2:-58230953 None:intergenic
GACATTACCGTGGCTCCAGT+TGG 0.484371 4_2:-58229266 None:intergenic
AACCATTGATGGCACTTTGG+TGG 0.486786 4_2:+58229100 Msa0610480:CDS
ACAAAATGTGACAGTTAAAA+CGG 0.489424 4_2:+58230372 Msa0610480:CDS
TTCATCTTTATTCCACTACA+TGG 0.491908 4_2:-58230848 None:intergenic
CTCAACTCCAAAATTCGAAC+CGG 0.494726 4_2:+58230095 Msa0610480:CDS
GGCATCCATTGAACGCTATG+TGG 0.499118 4_2:-58229977 None:intergenic
ATGTTCCACATAGCGTTCAA+TGG 0.499526 4_2:+58229972 Msa0610480:CDS
TGGACAAGGTACTGCCTTGT+GGG 0.500715 4_2:+58229214 Msa0610480:CDS
GAAAACATTGCATGTGGACC+GGG 0.509461 4_2:+58230164 Msa0610480:CDS
CGACGCCGCTTTCGCCGTAA+AGG 0.516630 4_2:-58228889 None:intergenic
ATTGCATGTGGACCGGGACA+TGG 0.516777 4_2:+58230170 Msa0610480:CDS
ATCCACAAATTACTTGTACC+AGG 0.519856 4_2:-58230140 None:intergenic
ATGAAACCAATTACCTGACC+GGG 0.524105 4_2:-58230553 None:intergenic
GACCTAGCAATGGATTTGTA+AGG 0.526984 4_2:+58230443 Msa0610480:CDS
ACATTACCGTGGCTCCAGTT+GGG 0.535346 4_2:-58229265 None:intergenic
TATGAAACCAATTACCTGAC+CGG 0.547446 4_2:-58230554 None:intergenic
TTCCTTACAAATCCATTGCT+AGG 0.550177 4_2:-58230445 None:intergenic
AAATTCCTTTACGGCGAAAG+CGG 0.552531 4_2:+58228884 Msa0610480:CDS
AGCCACCAAAGTGCCATCAA+TGG 0.553338 4_2:-58229102 None:intergenic
AAGAACCTTATTCCATGTCC+CGG 0.555681 4_2:-58230182 None:intergenic
ACCACTCACTTTAATTCCAG+AGG 0.562833 4_2:-58230753 None:intergenic
GGGGTAAATATCATAGCAGC+CGG 0.565921 4_2:+58230017 Msa0610480:CDS
GGCGGTGTGCTTGATGGACA+AGG 0.570853 4_2:+58229200 Msa0610480:CDS
GTTGACGGGGTTTCGATCCG+CGG 0.574746 4_2:+58229179 Msa0610480:CDS
AACTATGGAGCCAAGCCAGA+TGG 0.575309 4_2:+58228930 Msa0610480:CDS
ATGTAGTGGAATAAAGATGA+AGG 0.576564 4_2:+58230850 Msa0610480:CDS
TAATTGCCCAACTGGAGCCA+CGG 0.594866 4_2:+58229259 Msa0610480:CDS
GATTGTATCTCAATTGGCCC+TGG 0.595028 4_2:+58230122 Msa0610480:CDS
ATGGACAAGGTACTGCCTTG+TGG 0.600344 4_2:+58229213 Msa0610480:CDS
AGTTCGAAGTATCCATGTAG+TGG 0.607051 4_2:+58230836 Msa0610480:CDS
TCCACAAATTACTTGTACCA+GGG 0.607306 4_2:-58230139 None:intergenic
GCCTCTGGAATTAAAGTGAG+TGG 0.609415 4_2:+58230752 Msa0610480:CDS
GGATGGAAGTTAAATGAACC+AGG 0.616130 4_2:+58230346 Msa0610480:CDS
TGGATTGAAAACATTGCATG+TGG 0.620040 4_2:+58230158 Msa0610480:CDS
GGAAACAGCCCAAACACCGA+CGG 0.621233 4_2:+58230038 Msa0610480:CDS
CTTGGGAGGACATTTGGACG+TGG 0.621514 4_2:-58230064 None:intergenic
CCAAGTAAGAATTTCCCTTG+TGG 0.626504 4_2:-58229032 None:intergenic
AATTGAGATACAATCATCAC+CGG 0.627312 4_2:-58230114 None:intergenic
GCAGTATTGGAAGCTTGGGA+TGG 0.632087 4_2:+58230329 Msa0610480:intron
ATGTTCCTCGCCTTATCCGG+CGG 0.639177 4_2:+58228998 Msa0610480:CDS
CCAATGCAGCTCCACCAGCA+TGG 0.645581 4_2:-58230919 None:intergenic
TCTTGGGGTAGACCTAGCAA+TGG 0.646022 4_2:+58230433 Msa0610480:CDS
TGTGGTACGTAAATTGCCGC+CGG 0.646318 4_2:-58229014 None:intergenic
GCATCATGTAACCATGCTGG+TGG 0.657419 4_2:+58230908 Msa0610480:CDS
CATTTGGACGTGGATGCCGT+CGG 0.662987 4_2:-58230054 None:intergenic
GACGGGGTTTCGATCCGCGG+CGG 0.663495 4_2:+58229182 Msa0610480:CDS
GAAGTTTCAGCATGTTGACG+GGG 0.664703 4_2:+58229166 Msa0610480:CDS
AATTGCCGCCGGATAAGGCG+AGG 0.666819 4_2:-58229003 None:intergenic
GTCCATCAAGCACACCGCCG+CGG 0.670643 4_2:-58229196 None:intergenic
AATTATTGCCCAAACAACGA+AGG 0.671027 4_2:+58230526 Msa0610480:CDS
GTTGAGGATGGTGACTTGGG+AGG 0.672905 4_2:-58230078 None:intergenic
GAAGCATCATGTAACCATGC+TGG 0.675230 4_2:+58230905 Msa0610480:CDS
GCGGCAATTTACGTACCACA+AGG 0.682523 4_2:+58229017 Msa0610480:CDS
TGGTAAAACTGATACCACAA+AGG 0.706949 4_2:+58228950 Msa0610480:CDS
GGTGTTAGAATAAAGTCTTG+GGG 0.707177 4_2:+58230418 Msa0610480:CDS
CGGCAATTTACGTACCACAA+GGG 0.730607 4_2:+58229018 Msa0610480:CDS
TGAAACCAATTACCTGACCG+GGG 0.733003 4_2:-58230552 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATATGTCAATAAT+TGG + chr4_2:58229534-58229553 Msa0610480:intron 10.0%
!!! TTTTATAATGAGAATAATAA+TGG + chr4_2:58230289-58230308 Msa0610480:intron 10.0%
!! AATTAAAGATTAATAAAACC+AGG - chr4_2:58229349-58229368 None:intergenic 15.0%
!!! TTTTGAGTTTAATTATTTAG+TGG + chr4_2:58229406-58229425 Msa0610480:intron 15.0%
!!! TTTGAGTTTAATTATTTAGT+GGG + chr4_2:58229407-58229426 Msa0610480:intron 15.0%
!! AAATGTTATATACAAATAAG+TGG + chr4_2:58229657-58229676 Msa0610480:intron 15.0%
!!! TTTATTTTATTGAAGAAAAG+AGG + chr4_2:58229844-58229863 Msa0610480:intron 15.0%
!!! TGGTTAATTTTTTTTTTCTA+TGG + chr4_2:58229876-58229895 Msa0610480:intron 15.0%
!!! TTAATTTTTTTTTTCTATGG+TGG + chr4_2:58229879-58229898 Msa0610480:intron 15.0%
!! TCAAAAAATATTATGATTAC+CGG + chr4_2:58229927-58229946 Msa0610480:CDS 15.0%
!! TAAGATAATACTTTCTTAAA+AGG - chr4_2:58230240-58230259 None:intergenic 15.0%
!!! TTGTTACTTAAATTCCTTTA+CGG + chr4_2:58228875-58228894 Msa0610480:CDS 20.0%
!!! AATCTTTAATTTGAACAAGT+TGG + chr4_2:58229358-58229377 Msa0610480:intron 20.0%
!! ATTGGTTATATTATTCTTTC+TGG + chr4_2:58229552-58229571 Msa0610480:intron 20.0%
!! AAATTATAATCACGTTCTAT+TGG + chr4_2:58229733-58229752 Msa0610480:intron 20.0%
!!! TATCTGATTCAAATTTTAAC+CGG + chr4_2:58230617-58230636 Msa0610480:intron 20.0%
! GTGATTATGATTAAGAGAAA+AGG - chr4_2:58228849-58228868 None:intergenic 25.0%
! ACATACAATGTAATCAACTA+TGG + chr4_2:58228915-58228934 Msa0610480:CDS 25.0%
! ATTTCTAAATGTATGGTCTA+AGG + chr4_2:58228974-58228993 Msa0610480:CDS 25.0%
! TAAAACCAGGCAAATAAATA+AGG - chr4_2:58229336-58229355 None:intergenic 25.0%
! TTTAATTATTTAGTGGGAGT+AGG + chr4_2:58229413-58229432 Msa0610480:intron 25.0%
! ATAGAATGATATACTAGTGT+AGG + chr4_2:58229681-58229700 Msa0610480:intron 25.0%
!! TTGAATATTTGTTGCAGTAT+TGG + chr4_2:58230316-58230335 Msa0610480:intron 25.0%
! ACAAAATGTGACAGTTAAAA+CGG + chr4_2:58230372-58230391 Msa0610480:CDS 25.0%
! AATTCTGGTCAATTACAATT+GGG - chr4_2:58230510-58230529 None:intergenic 25.0%
! TAATTCTGGTCAATTACAAT+TGG - chr4_2:58230511-58230530 None:intergenic 25.0%
! TTGGTTTCATAACATCTTAT+TGG + chr4_2:58230566-58230585 Msa0610480:intron 25.0%
! TCAAACAAAATTGATGTAAC+CGG - chr4_2:58230639-58230658 None:intergenic 25.0%
! GTGGTTGAAATTATTAAATC+AGG + chr4_2:58230730-58230749 Msa0610480:intron 25.0%
TACATTTAGAAATGCCTTTG+TGG - chr4_2:58228967-58228986 None:intergenic 30.0%
!! CAAAGGCATTTCTAAATGTA+TGG + chr4_2:58228967-58228986 Msa0610480:CDS 30.0%
TAACCGGTAAAAATGGTAAT+TGG + chr4_2:58229141-58229160 Msa0610480:CDS 30.0%
!! CAACCAATTACCATTTTTAC+CGG - chr4_2:58229147-58229166 None:intergenic 30.0%
CTTGTGGGATTGTAAAAATT+CGG + chr4_2:58229229-58229248 Msa0610480:CDS 30.0%
! TAGGCAATGATATAATGGAA+CGG + chr4_2:58229432-58229451 Msa0610480:intron 30.0%
ATACTTAATATGCAACTGCT+TGG + chr4_2:58229460-58229479 Msa0610480:intron 30.0%
GTATATAGAAGTTGAAGCAT+GGG + chr4_2:58229493-58229512 Msa0610480:intron 30.0%
!! ATTGGTTTGCGTAATATTGA+AGG - chr4_2:58229600-58229619 None:intergenic 30.0%
TATGTCTGATCAATTCGAAT+TGG - chr4_2:58229618-58229637 None:intergenic 30.0%
TCACGTTCTATTGGAATTAT+TGG + chr4_2:58229742-58229761 Msa0610480:intron 30.0%
TGCCAAAATGTAAAAACACA+AGG + chr4_2:58229996-58230015 Msa0610480:CDS 30.0%
GCCAAAATGTAAAAACACAA+GGG + chr4_2:58229997-58230016 Msa0610480:CDS 30.0%
CCAAAATGTAAAAACACAAG+GGG + chr4_2:58229998-58230017 Msa0610480:CDS 30.0%
AATTGAGATACAATCATCAC+CGG - chr4_2:58230117-58230136 None:intergenic 30.0%
! ATGGTGTTAGAATAAAGTCT+TGG + chr4_2:58230416-58230435 Msa0610480:CDS 30.0%
! TGGTGTTAGAATAAAGTCTT+GGG + chr4_2:58230417-58230436 Msa0610480:CDS 30.0%
! ACATCAACTTTGTTTGACTA+GGG - chr4_2:58230598-58230617 None:intergenic 30.0%
! TACATCAACTTTGTTTGACT+AGG - chr4_2:58230599-58230618 None:intergenic 30.0%
!! GAGCTGATTATATGTTTTTG+TGG + chr4_2:58230711-58230730 Msa0610480:intron 30.0%
AAATTATTAAATCAGGCCTC+TGG + chr4_2:58230737-58230756 Msa0610480:intron 30.0%
GTAACATACCAAAACATTCA+TGG + chr4_2:58230776-58230795 Msa0610480:CDS 30.0%
TTCATCTTTATTCCACTACA+TGG - chr4_2:58230851-58230870 None:intergenic 30.0%
! ATGTAGTGGAATAAAGATGA+AGG + chr4_2:58230850-58230869 Msa0610480:CDS 30.0%
TGGTAAAACTGATACCACAA+AGG + chr4_2:58228950-58228969 Msa0610480:CDS 35.0%
! GCTATTTCCATAACCATTGA+TGG + chr4_2:58229089-58229108 Msa0610480:CDS 35.0%
TCATCTAATTACCGTGTAAC+CGG + chr4_2:58229125-58229144 Msa0610480:CDS 35.0%
!! TACCATTTTTACCGGTTACA+CGG - chr4_2:58229139-58229158 None:intergenic 35.0%
TTGAAGTTTCAGCATGTTGA+CGG + chr4_2:58229164-58229183 Msa0610480:CDS 35.0%
! CGAATTTTTACAATCCCACA+AGG - chr4_2:58229231-58229250 None:intergenic 35.0%
GAGATAATTAGACATTACCG+TGG - chr4_2:58229279-58229298 None:intergenic 35.0%
! TTGCACCTTATTTATTTGCC+TGG + chr4_2:58229328-58229347 Msa0610480:intron 35.0%
GGTATATAGAAGTTGAAGCA+TGG + chr4_2:58229492-58229511 Msa0610480:intron 35.0%
TCAGACATAAGAATCAGAAG+AGG + chr4_2:58229629-58229648 Msa0610480:intron 35.0%
GTACAAAGCAAAAAGCAACT+TGG - chr4_2:58229795-58229814 None:intergenic 35.0%
ACTATTCATCGATGTCAAAC+CGG - chr4_2:58229949-58229968 None:intergenic 35.0%
!! CCCCTTGTGTTTTTACATTT+TGG - chr4_2:58230001-58230020 None:intergenic 35.0%
GGTGATGATTGTATCTCAAT+TGG + chr4_2:58230116-58230135 Msa0610480:CDS 35.0%
TCCACAAATTACTTGTACCA+GGG - chr4_2:58230142-58230161 None:intergenic 35.0%
ATCCACAAATTACTTGTACC+AGG - chr4_2:58230143-58230162 None:intergenic 35.0%
TGGATTGAAAACATTGCATG+TGG + chr4_2:58230158-58230177 Msa0610480:CDS 35.0%
GTTAAAACGGTTACATTCAC+AGG + chr4_2:58230385-58230404 Msa0610480:CDS 35.0%
ACATTCACAGGAACTCAAAA+TGG + chr4_2:58230397-58230416 Msa0610480:CDS 35.0%
GGTGTTAGAATAAAGTCTTG+GGG + chr4_2:58230418-58230437 Msa0610480:CDS 35.0%
TTCCTTACAAATCCATTGCT+AGG - chr4_2:58230448-58230467 None:intergenic 35.0%
! CTTTTTCCAAGATGCAATCA+TGG + chr4_2:58230471-58230490 Msa0610480:CDS 35.0%
CATTAACCATGATTGCATCT+TGG - chr4_2:58230480-58230499 None:intergenic 35.0%
! GGTCAATTACAATTGGGTTT+TGG - chr4_2:58230504-58230523 None:intergenic 35.0%
AATTATTGCCCAAACAACGA+AGG + chr4_2:58230526-58230545 Msa0610480:CDS 35.0%
TATGAAACCAATTACCTGAC+CGG - chr4_2:58230557-58230576 None:intergenic 35.0%
! CAGATGTTCCATGAATGTTT+TGG - chr4_2:58230787-58230806 None:intergenic 35.0%
AAATTCCTTTACGGCGAAAG+CGG + chr4_2:58228884-58228903 Msa0610480:CDS 40.0%
! ATCAGTTTTACCATCTGGCT+TGG - chr4_2:58228943-58228962 None:intergenic 40.0%
! GTGGTATCAGTTTTACCATC+TGG - chr4_2:58228948-58228967 None:intergenic 40.0%
CCAAGTAAGAATTTCCCTTG+TGG - chr4_2:58229035-58229054 None:intergenic 40.0%
CCACAAGGGAAATTCTTACT+TGG + chr4_2:58229032-58229051 Msa0610480:CDS 40.0%
CTTGGAACTGCAACATTTAG+TGG + chr4_2:58229050-58229069 Msa0610480:CDS 40.0%
! CAAAGTGCCATCAATGGTTA+TGG - chr4_2:58229099-58229118 None:intergenic 40.0%
! CATAACCATTGATGGCACTT+TGG + chr4_2:58229097-58229116 Msa0610480:CDS 40.0%
TACCGTGTAACCGGTAAAAA+TGG + chr4_2:58229134-58229153 Msa0610480:CDS 40.0%
TGAAGTTTCAGCATGTTGAC+GGG + chr4_2:58229165-58229184 Msa0610480:CDS 40.0%
GACAAAAGTAATTGCCCAAC+TGG + chr4_2:58229251-58229270 Msa0610480:CDS 40.0%
! GAACAAGTTGGAGTTAACTC+AGG + chr4_2:58229370-58229389 Msa0610480:intron 40.0%
GGGAGTAGGCAATGATATAA+TGG + chr4_2:58229427-58229446 Msa0610480:intron 40.0%
!! AAGAAAAGAGGTGTTGACAG+TGG + chr4_2:58229856-58229875 Msa0610480:intron 40.0%
ATGTTCCACATAGCGTTCAA+TGG + chr4_2:58229972-58229991 Msa0610480:CDS 40.0%
!!! TCGAATTTTGGAGTTGAGGA+TGG - chr4_2:58230093-58230112 None:intergenic 40.0%
CTCAACTCCAAAATTCGAAC+CGG + chr4_2:58230095-58230114 Msa0610480:CDS 40.0%
!!! ATCATCACCGGTTCGAATTT+TGG - chr4_2:58230105-58230124 None:intergenic 40.0%
TGAAAACATTGCATGTGGAC+CGG + chr4_2:58230163-58230182 Msa0610480:CDS 40.0%
AAGAACCTTATTCCATGTCC+CGG - chr4_2:58230185-58230204 None:intergenic 40.0%
! TTGTTGCAGTATTGGAAGCT+TGG + chr4_2:58230324-58230343 Msa0610480:intron 40.0%
! TGTTGCAGTATTGGAAGCTT+GGG + chr4_2:58230325-58230344 Msa0610480:intron 40.0%
GGATGGAAGTTAAATGAACC+AGG + chr4_2:58230346-58230365 Msa0610480:CDS 40.0%
! ACTGTCACATTTTGTACTCC+TGG - chr4_2:58230367-58230386 None:intergenic 40.0%
! GACCTAGCAATGGATTTGTA+AGG + chr4_2:58230443-58230462 Msa0610480:CDS 40.0%
CGTTGTTTGGGCAATAATTC+TGG - chr4_2:58230525-58230544 None:intergenic 40.0%
ATGAAACCAATTACCTGACC+GGG - chr4_2:58230556-58230575 None:intergenic 40.0%
! AACTTTGTTTGACTAGGGTG+AGG - chr4_2:58230593-58230612 None:intergenic 40.0%
ACCACTCACTTTAATTCCAG+AGG - chr4_2:58230756-58230775 None:intergenic 40.0%
! AGTTCGAAGTATCCATGTAG+TGG + chr4_2:58230836-58230855 Msa0610480:CDS 40.0%
GCTCACTTACAAAAACCAAG+TGG + chr4_2:58230880-58230899 Msa0610480:CDS 40.0%
CGGCAATTTACGTACCACAA+GGG + chr4_2:58229018-58229037 Msa0610480:CDS 45.0%
! AACCATTGATGGCACTTTGG+TGG + chr4_2:58229100-58229119 Msa0610480:CDS 45.0%
GAAGTTTCAGCATGTTGACG+GGG + chr4_2:58229166-58229185 Msa0610480:CDS 45.0%
GGGGTAAATATCATAGCAGC+CGG + chr4_2:58230017-58230036 Msa0610480:CDS 45.0%
!!! CGGTTCGAATTTTGGAGTTG+AGG - chr4_2:58230097-58230116 None:intergenic 45.0%
GATTGTATCTCAATTGGCCC+TGG + chr4_2:58230122-58230141 Msa0610480:CDS 45.0%
GCCCTGGTACAAGTAATTTG+TGG + chr4_2:58230138-58230157 Msa0610480:CDS 45.0%
GAAAACATTGCATGTGGACC+GGG + chr4_2:58230164-58230183 Msa0610480:CDS 45.0%
TGAAACCAATTACCTGACCG+GGG - chr4_2:58230555-58230574 None:intergenic 45.0%
!! GCCTCTGGAATTAAAGTGAG+TGG + chr4_2:58230752-58230771 Msa0610480:CDS 45.0%
TACATGATGCTTCAGCCACT+TGG - chr4_2:58230898-58230917 None:intergenic 45.0%
! GAAGCATCATGTAACCATGC+TGG + chr4_2:58230905-58230924 Msa0610480:CDS 45.0%
AACTATGGAGCCAAGCCAGA+TGG + chr4_2:58228930-58228949 Msa0610480:CDS 50.0%
ACGTAAATTGCCGCCGGATA+AGG - chr4_2:58229011-58229030 None:intergenic 50.0%
TGTGGTACGTAAATTGCCGC+CGG - chr4_2:58229017-58229036 None:intergenic 50.0%
GCGGCAATTTACGTACCACA+AGG + chr4_2:58229017-58229036 Msa0610480:CDS 50.0%
! AGCCACCAAAGTGCCATCAA+TGG - chr4_2:58229105-58229124 None:intergenic 50.0%
!! ATGGACAAGGTACTGCCTTG+TGG + chr4_2:58229213-58229232 Msa0610480:CDS 50.0%
!! TGGACAAGGTACTGCCTTGT+GGG + chr4_2:58229214-58229233 Msa0610480:CDS 50.0%
TAATTGCCCAACTGGAGCCA+CGG + chr4_2:58229259-58229278 Msa0610480:CDS 50.0%
ACATTACCGTGGCTCCAGTT+GGG - chr4_2:58229268-58229287 None:intergenic 50.0%
GCAACTGCTTGGTCTAGTTG+CGG + chr4_2:58229471-58229490 Msa0610480:intron 50.0%
! CTATGGTGGTTTTCAGACGC+TGG + chr4_2:58229893-58229912 Msa0610480:intron 50.0%
GGCATCCATTGAACGCTATG+TGG - chr4_2:58229980-58229999 None:intergenic 50.0%
! TGGTGACTTGGGAGGACATT+TGG - chr4_2:58230073-58230092 None:intergenic 50.0%
GGAGTTGAGGATGGTGACTT+GGG - chr4_2:58230084-58230103 None:intergenic 50.0%
TGGAGTTGAGGATGGTGACT+TGG - chr4_2:58230085-58230104 None:intergenic 50.0%
! GCAGTATTGGAAGCTTGGGA+TGG + chr4_2:58230329-58230348 Msa0610480:intron 50.0%
TCTTGGGGTAGACCTAGCAA+TGG + chr4_2:58230433-58230452 Msa0610480:CDS 50.0%
GCATCATGTAACCATGCTGG+TGG + chr4_2:58230908-58230927 Msa0610480:CDS 50.0%
GGCATGTTCCTCGCCTTATC+CGG + chr4_2:58228995-58229014 Msa0610480:CDS 55.0%
ATGTTCCTCGCCTTATCCGG+CGG + chr4_2:58228998-58229017 Msa0610480:CDS 55.0%
GACATTACCGTGGCTCCAGT+TGG - chr4_2:58229269-58229288 None:intergenic 55.0%
! GTCGGTGTTTGGGCTGTTTC+CGG - chr4_2:58230039-58230058 None:intergenic 55.0%
GGAAACAGCCCAAACACCGA+CGG + chr4_2:58230038-58230057 Msa0610480:CDS 55.0%
CATTTGGACGTGGATGCCGT+CGG - chr4_2:58230057-58230076 None:intergenic 55.0%
CTTGGGAGGACATTTGGACG+TGG - chr4_2:58230067-58230086 None:intergenic 55.0%
! GTTGAGGATGGTGACTTGGG+AGG - chr4_2:58230081-58230100 None:intergenic 55.0%
ATTGCATGTGGACCGGGACA+TGG + chr4_2:58230170-58230189 Msa0610480:CDS 55.0%
GTGGACCGGGACATGGAATA+AGG + chr4_2:58230177-58230196 Msa0610480:CDS 55.0%
CATGCTGGTGGAGCTGCATT+GGG + chr4_2:58230920-58230939 Msa0610480:CDS 55.0%
AATTGCCGCCGGATAAGGCG+AGG - chr4_2:58229006-58229025 None:intergenic 60.0%
GTTGACGGGGTTTCGATCCG+CGG + chr4_2:58229179-58229198 Msa0610480:CDS 60.0%
! GGCGGTGTGCTTGATGGACA+AGG + chr4_2:58229200-58229219 Msa0610480:CDS 60.0%
! CGTGGATGCCGTCGGTGTTT+GGG - chr4_2:58230049-58230068 None:intergenic 60.0%
! ACGTGGATGCCGTCGGTGTT+TGG - chr4_2:58230050-58230069 None:intergenic 60.0%
CGGGGCAGCCTTCGTTGTTT+GGG - chr4_2:58230537-58230556 None:intergenic 60.0%
CCAAACAACGAAGGCTGCCC+CGG + chr4_2:58230535-58230554 Msa0610480:CDS 60.0%
CCAATGCAGCTCCACCAGCA+TGG - chr4_2:58230922-58230941 None:intergenic 60.0%
CCATGCTGGTGGAGCTGCAT+TGG + chr4_2:58230919-58230938 Msa0610480:CDS 60.0%
! CGACGCCGCTTTCGCCGTAA+AGG - chr4_2:58228892-58228911 None:intergenic 65.0%
ATCCGCGGCGGTGTGCTTGA+TGG + chr4_2:58229194-58229213 Msa0610480:CDS 65.0%
GTCCATCAAGCACACCGCCG+CGG - chr4_2:58229199-58229218 None:intergenic 65.0%
CCGGGGCAGCCTTCGTTGTT+TGG - chr4_2:58230538-58230557 None:intergenic 65.0%
GGCTGCCCCGGTCAGGTAAT+TGG + chr4_2:58230547-58230566 Msa0610480:intron 65.0%
GACGGGGTTTCGATCCGCGG+CGG + chr4_2:58229182-58229201 Msa0610480:CDS 70.0%
CAACGAAGGCTGCCCCGGTC+AGG + chr4_2:58230540-58230559 Msa0610480:CDS 70.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 58228822 58230970 58228822 ID=Msa0610480;Name=Msa0610480
chr4_2 mRNA 58228822 58230970 58228822 ID=Msa0610480-mRNA-1;Parent=Msa0610480;Name=Msa0610480-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0.33|0|1|1|1|4|0|398
chr4_2 exon 58228822 58229280 58228822 ID=Msa0610480-mRNA-1:exon:15271;Parent=Msa0610480-mRNA-1
chr4_2 exon 58229909 58230198 58229909 ID=Msa0610480-mRNA-1:exon:15272;Parent=Msa0610480-mRNA-1
chr4_2 exon 58230333 58230561 58230333 ID=Msa0610480-mRNA-1:exon:15273;Parent=Msa0610480-mRNA-1
chr4_2 exon 58230752 58230970 58230752 ID=Msa0610480-mRNA-1:exon:15274;Parent=Msa0610480-mRNA-1
chr4_2 CDS 58228822 58229280 58228822 ID=Msa0610480-mRNA-1:cds;Parent=Msa0610480-mRNA-1
chr4_2 CDS 58229909 58230198 58229909 ID=Msa0610480-mRNA-1:cds;Parent=Msa0610480-mRNA-1
chr4_2 CDS 58230333 58230561 58230333 ID=Msa0610480-mRNA-1:cds;Parent=Msa0610480-mRNA-1
chr4_2 CDS 58230752 58230970 58230752 ID=Msa0610480-mRNA-1:cds;Parent=Msa0610480-mRNA-1
Gene Sequence

>Msa0610480

ATGTCAAAACTTAACAATCTTCTTCCTTTTCTCTTAATCATAATCACATTATCTTGTTACTTAAATTCCTTTACGGCGAAAGCGGCGTCGTCGACATACAATGTAATCAACTATGGAGCCAAGCCAGATGGTAAAACTGATACCACAAAGGCATTTCTAAATGTATGGTCTAAGGCATGTTCCTCGCCTTATCCGGCGGCAATTTACGTACCACAAGGGAAATTCTTACTTGGAACTGCAACATTTAGTGGAAAATGTATCAACAAAGCTATTTCCATAACCATTGATGGCACTTTGGTGGCTTCATCTAATTACCGTGTAACCGGTAAAAATGGTAATTGGTTGAAGTTTCAGCATGTTGACGGGGTTTCGATCCGCGGCGGTGTGCTTGATGGACAAGGTACTGCCTTGTGGGATTGTAAAAATTCGGACAAAAGTAATTGCCCAACTGGAGCCACGACGCTGGAATTCAGTAGCTCAAAAAATATTATGATTACCGGTTTGACATCGATGAATAGTCAAATGTTCCACATAGCGTTCAATGGATGCCAAAATGTAAAAACACAAGGGGTAAATATCATAGCAGCCGGAAACAGCCCAAACACCGACGGCATCCACGTCCAAATGTCCTCCCAAGTCACCATCCTCAACTCCAAAATTCGAACCGGTGATGATTGTATCTCAATTGGCCCTGGTACAAGTAATTTGTGGATTGAAAACATTGCATGTGGACCGGGACATGGAATAAGTATTGGAAGCTTGGGATGGAAGTTAAATGAACCAGGAGTACAAAATGTGACAGTTAAAACGGTTACATTCACAGGAACTCAAAATGGTGTTAGAATAAAGTCTTGGGGTAGACCTAGCAATGGATTTGTAAGGAACATCTTTTTCCAAGATGCAATCATGGTTAATGTCCAAAACCCAATTGTAATTGACCAGAATTATTGCCCAAACAACGAAGGCTGCCCCGGTCAGGCCTCTGGAATTAAAGTGAGTGGTGTAACATACCAAAACATTCATGGAACATCTGCAACACAAGTAGCTGTGAAATTTGATTGTAGTTCGAAGTATCCATGTAGTGGAATAAAGATGAAGGATGTAAAGCTCACTTACAAAAACCAAGTGGCTGAAGCATCATGTAACCATGCTGGTGGAGCTGCATTGGGTTCAGTTCAGCCAGAGAGTTGCTTATAG
Protein sequence

>Msa0610480

MSKLNNLLPFLLIIITLSCYLNSFTAKAASSTYNVINYGAKPDGKTDTTKAFLNVWSKACSSPYPAAIYVPQGKFLLGTATFSGKCINKAISITIDGTLVASSNYRVTGKNGNWLKFQHVDGVSIRGGVLDGQGTALWDCKNSDKSNCPTGATTLEFSSSKNIMITGLTSMNSQMFHIAFNGCQNVKTQGVNIIAAGNSPNTDGIHVQMSSQVTILNSKIRTGDDCISIGPGTSNLWIENIACGPGHGISIGSLGWKLNEPGVQNVTVKTVTFTGTQNGVRIKSWGRPSNGFVRNIFFQDAIMVNVQNPIVIDQNYCPNNEGCPGQASGIKVSGVTYQNIHGTSATQVAVKFDCSSKYPCSGIKMKDVKLTYKNQVAEASCNHAGGAALGSVQPESCL*